CN116114589A - Breeding method for identifying color of colored rape petals based on spectral characteristics - Google Patents
Breeding method for identifying color of colored rape petals based on spectral characteristics Download PDFInfo
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- CN116114589A CN116114589A CN202211468548.XA CN202211468548A CN116114589A CN 116114589 A CN116114589 A CN 116114589A CN 202211468548 A CN202211468548 A CN 202211468548A CN 116114589 A CN116114589 A CN 116114589A
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- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000003595 spectral effect Effects 0.000 title claims abstract description 14
- 239000000049 pigment Substances 0.000 claims abstract description 17
- 238000002791 soaking Methods 0.000 claims abstract description 13
- 238000009331 sowing Methods 0.000 claims abstract description 12
- 238000001228 spectrum Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 208000035240 Disease Resistance Diseases 0.000 claims description 4
- 230000009418 agronomic effect Effects 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 claims description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 3
- 230000008685 targeting Effects 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 238000012850 discrimination method Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 19
- 239000003086 colorant Substances 0.000 description 10
- 239000001666 citrus aurantium l. flower Substances 0.000 description 4
- 240000007164 Salvia officinalis Species 0.000 description 2
- 235000002912 Salvia officinalis Nutrition 0.000 description 2
- 229930002877 anthocyanin Natural products 0.000 description 2
- 235000010208 anthocyanin Nutrition 0.000 description 2
- 239000004410 anthocyanin Substances 0.000 description 2
- 150000004636 anthocyanins Chemical class 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 235000002020 sage Nutrition 0.000 description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 244000172419 Hemerocallis flava Species 0.000 description 1
- 235000017209 Hemerocallis flava Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- ONTQJDKFANPPKK-UHFFFAOYSA-L chembl3185981 Chemical compound [Na+].[Na+].CC1=CC(C)=C(S([O-])(=O)=O)C=C1N=NC1=CC(S([O-])(=O)=O)=C(C=CC=C2)C2=C1O ONTQJDKFANPPKK-UHFFFAOYSA-L 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The invention discloses a breeding method for identifying color of color rape petals based on spectral characteristics, which comprises the following steps: selecting petals of a color oil menu plant, placing the petals into a centrifuge tube filled with an extracting agent, sealing the centrifuge tube, and soaking the centrifuge tube in a dark place; mixing the soaking solution uniformly, taking 1mL, performing full-band scanning, and recording the peak value of each band; naming the color rape according to the spectrum characteristics of the mottle pigment, and recording; the named color rape is hung as female parent, yellow rape flower is selected as male parent, and hybridized to obtain F 0 Seed; will F 0 Sowing seeds, and selecting excellent single plant main inflorescence seed reserving to obtain F l Seed, F 1 Seed mixed sowing; performing multiple generations of self-serviceCross-segregating until F 6 And starting the individual cells to appear the lines with the same target color of the whole cell, namely carrying out selfing seed reserving on the cells. The invention adopts an accurate color discrimination method, directly records each generation accurately according to the spectrum, and targets and selects target single plants in a targeting way, thereby accelerating the homozygosity of breeding materials.
Description
Technical Field
The invention belongs to the technical field of rape flower breeding, and particularly relates to a breeding method for identifying color of color rape petals based on spectral characteristics.
Background
The main reason why the color rape petals are different in color is that pigment components in the petals are not identical. The existing research shows that: the color rape petal pigment has complex fine components, but mainly contains anthocyanin, carotenoid and the like in flavonoids. In nature, anthocyanin and carotenoid are very many, and especially flavonoid approaches 5000 kinds. The invention patent with the application number of CN201510329353.0 discloses a breeding method and a planting method for colored rape for creative agriculture, and rape flowers with various flower varieties can be obtained, but in the breeding process, information errors can be formed when offspring are planted by selecting single plants due to the difficulty of distinguishing and accurately naming colors of rape flowers with the same color system by naked eyes in the labeling process.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a breeding method for identifying the color of color rape petals based on spectral characteristics.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
the breeding method for identifying the color of the colored rape petals based on the spectral characteristics comprises the following steps:
s1, selecting a color oil menu plant with excellent plant growth vigor and 1-3 flowers, taking 4-8 petals of flowers blooming on the same day, placing the petals into a brown centrifuge tube filled with distilled water and/or methanol reagent, sealing, and soaking in a dark place for 2 days;
s2, uniformly mixing the soaking solution, taking 1mL, performing full-band scanning, and recording the peak value of each band;
s3, naming the color rape according to the spectrum characteristics of the mottle pigment, and recording;
s4, hanging the named color rape as a female parent, selecting yellow rape flowers as male parents, and hybridizing to obtain F 0 Seed;
s5, F 0 Sowing seeds, and selecting excellent single plant main inflorescence seed reserving to obtain F l Seed, F 1 Seed mixed sowing from F 2 Initially, adopting a petal pigment detection method in the initial flowering period, selecting a color rape single plant with the same spectrum as the initial color single plant, recording, hanging the single plant, and reserving seeds by selfing bags;
s6, carrying out multi-generation selfing separation until F 6 And starting to appear the lines with the same target color of the whole cell in the individual cell, namely carrying out selfing seed reserving on the cell, and selecting the lines with excellent agronomic characters and strong lodging resistance from the lines with the target colors for seed reserving.
Further, in the step S1, the petals are uniformly mixed with the soaking solution for 1 time during the light-shielding soaking period.
Further, in step S2, full-band scanning is performed on an ultraviolet-visible spectrophotometer.
Further, in the step S4, yellow rape flowers with excellent growth vigor, lodging resistance and strong disease resistance are selected as male parents for hybridization.
Further, in step S6, seed reserving is performed by bagging or billing.
The beneficial effects of the invention are as follows:
1. in selecting the color of petals of an initially colored canolaAnd at F 2 When the color oil menu strain is selected in a generation way, the colors of petals of the single strain are very easy to be inconsistent due to generation separation (1 year), and errors caused by judging the colors and memorizing can be avoided by naked eyes through the method.
2. At F 2 Because a series of homochromatic single plants with different colors can be generated after the color rape and the yellow rape are hybridized, at the moment, the color error is large in naked eye judgment, the recording is very difficult, the colors are easy to confuse, the offspring are not easy to homozygously, and the breeding period is prolonged. The invention adopts an accurate color discrimination method, directly records each generation accurately according to the spectrum, and targets and selects target single plants in a targeting way, thereby accelerating the homozygosity of breeding materials.
3. From F 2 At the beginning of generation, accurate detection method and record are adopted, the color selection of each generation can be kept consistent, and errors caused by naked eye judgment are eliminated.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
In this embodiment, the petal color can be accurately identified based on the spectral characteristics of the stain pigment.
Specifically, distilled water or methanol can be used as pigment extractant of the colored rape petals to distinguish colored rape with different colors.
(1) Distilled water as color rape petal extractant
Distilled water is used as an extracting agent of petal pigment, and pigment in purple petals cannot be extracted. The red rape flower has a specific wave band at 326.5 nm; the deep orange rape has a specific wave band at 320.5; both the golden rape flowers and the golden rape flowers have specific wave bands at 334.5nm, but the golden rape flowers have specific wave bands at 531.0nm, and the golden rape flowers do not; white rape flowers and orange yellow daylily have specific wave bands at 535.0nm and 531.0nm respectively, but white rape flowers do not have specific wave bands at 334.5nm, and orange yellow rape flowers have; yellow, pink and light orange flowers have specific bands at 344.0nm, 346.5nm and 345.5nm, respectively, but pink and light orange flowers have specific bands at 554.5nm and 555.5nm, respectively, whereas yellow flowers do not have specific bands at 255.5nm in the case of pink and light orange flowers, and thus yellow, pink and light orange flowers can be distinguished according to the number of specific bands (table 1).
TABLE 1 band distribution of distilled water as a pigment extractant for petals of a color rape flower
(2) Methanol as pigment extractant for colour rape petals
Methanol is used as pigment of colored rape petals for extraction, and the purple rape has a specific wave band at 320 nm; yellow rape has specific wave bands at 388nm, 357nm and 310.5 nm; orange rape has specific wave bands at 442nm and 327.5 nm; the comparison was made with purple rape flower, yellow rape flower and orange rape flower as the reference. For example, the red rape flowers have more 350.5nm band than the yellow rape flowers; white rape flower contains a specific wave band of 535.0nm besides a wave band of 350.5 nm; both the common sage herb flower and the yellow common sage herb flower have 400nm wave bands, but other colors are absent; both the white orange rape flower and the light orange rape flower have no 266.0nm wave band, but the light orange rape flower has 414.0nm wave band less than the white orange rape flower; the specific wave bands of the light orange rape flowers are consistent with those of the deep orange rape flowers, but the specific wave bands of the deep orange rape flowers are more than 265.5 nm. Therefore, the other colors can be clearly distinguished by using only yellow rape flowers as a control (Table 2).
TABLE 2 band distribution of methanol as a color rape flower petal pigment extractant
Examples
A breeding method for identifying color of colored rape petals based on spectral characteristics (taking cultivation of orange rape as an example), which comprises the following steps:
s1, selecting red rape flowers (Zhehu red No. 1) with excellent plant growth vigor and flowering of 1-3 flowers, taking 4-8 petals of the flowers on the same day, putting the petals into a brown centrifuge tube filled with distilled water and/or methanol reagent, sealing, soaking for 2 days in a dark place, and uniformly mixing the soaking liquid for 1 time.
S2, uniformly mixing the soaking solution, taking 1mL, performing full-band scanning on an ultraviolet-visible spectrophotometer, and recording the peak value of each band (the safflower band value in table 2);
s3, naming the color rape (deep orange, white orange and light orange) according to the spectrum characteristics of the petal pigment, and recording.
S4, using the color rape hanging plate named in S3 as a female parent, selecting yellow rape flower (Zhejiang oil 51) with excellent growth vigor, lodging resistance and strong disease resistance as a male parent, and hybridizing to obtain F 0 Seed;
s5, F 0 Sowing seeds, and selecting excellent single plant main inflorescence seed reserving to obtain F l Seed, F 1 Seed mixed sowing from F 2 Initially, adopting a petal pigment detection method in the initial flowering period, selecting a color rape single plant with the same spectrum as the initial color single plant, recording, hanging the single plant, and reserving seeds by selfing bags;
s6, carrying out multi-generation selfing separation until F 6 And (3) starting to appear the strain (the homozygosity rate reaches 100%) of the same target color of the whole cell in the individual cell, namely, carrying out selfing seed reserving on the cell, and then selecting the strain with excellent agronomic characters and strong lodging resistance from the strain with the target color for bagging or seed reserving.
Comparative example
A breeding method for identifying color of color rape petals on the basis of non-spectral features comprises the following steps:
c1, selecting a colored oil menu plant with excellent plant growth vigor and 1-3 flowers of flowers, and naming a card as a female parent; selecting excellent growth vigor and lodging resistanceAnd yellow rape flower with strong disease resistance as male parent to hybridize to obtain F 0 Seed;
c2, F 0 Sowing seeds, and selecting excellent single plant main inflorescence seed reserving to obtain F l Seed, F 1 Seed mixed sowing;
c3, from F 2 Initially, the petal color of the canola population begins to separate. Because the color rape petals are controlled by quantitative traits, breeders mark single plants according to naked eye judgment results, record with orange 1 and orange 2 … …, and meanwhile, need to take a photograph for preservation, and the color rape petals are F 3 Color comparison is carried out before seed sowing.
C4, selecting F to be sown 3 And (5) replacing single plant seeds and sowing. Repeat F 2 And selecting a single plant. Because of larger error in the color photographed according to the naked eye comparison, F 3 Orange of the single plant petals is continuously separated, the orange is slightly deep or light, and the field resolution is not easy to realize by naked eyes; in addition, different mobile phones are photographed and have the problem of chromatic aberration, so that the accuracy of the color rape petal targeting is lower. At least 2 generations of selection work are added under the condition of stable and stable target color of the petals of the population, and the target color is possibly not completely consistent.
C5, carrying out multi-generation selfing separation until F 8 And starting to appear the lines with the same target color of the whole cell in the individual cell, namely carrying out selfing seed reserving on the cell, and selecting the lines with excellent agronomic characters and strong lodging resistance from the lines with the target colors for bagging or covering and seed reserving.
The error caused by the judgment of the color and the memory by naked eyes can be avoided by the method. And the target single plant is targeted and selected directly according to the spectrum, each generation of accurate record, and the homozygosity of the breeding material is quickened. From F 2 At the beginning of generation, accurate detection method and record are adopted, the color selection of each generation can be kept consistent, and errors caused by naked eye judgment are eliminated.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (5)
1. A breeding method for identifying color of color rape petals based on spectral characteristics is characterized by comprising the following steps:
s1, selecting a color oil menu plant with excellent plant growth vigor and 1-3 flowers, taking 4-8 petals of flowers blooming on the same day, placing the petals into a brown centrifuge tube filled with distilled water and/or methanol reagent, sealing, and soaking in a dark place for 2 days;
s2, uniformly mixing the soaking solution, taking 1mL, performing full-band scanning, and recording the peak value of each band;
s3, naming the color rape according to the spectrum characteristics of the mottle pigment, and recording;
s4, hanging the named color rape as a female parent, selecting yellow rape flowers as male parents, and hybridizing to obtain F 0 Seed;
s5, F 0 Sowing seeds, and selecting excellent single plant main inflorescence seed reserving to obtain F l Seed, F 1 Seed mixed sowing from F 2 Initially, adopting a petal pigment detection method in the initial flowering period, selecting a color rape single plant with the same spectrum as the initial color single plant, recording, hanging the single plant, and reserving seeds by selfing bags;
s6, carrying out multi-generation selfing separation until F 6 Starting individual district to appear the strain with the same target color of the whole district, namely, carrying out selfing seed reserving on the districtAnd selecting a strain with excellent agronomic characters and strong lodging resistance from the target flower color strain for seed reservation.
2. The breeding method for identifying color of color rape petals based on spectral features according to claim 1, wherein in the step S1, the soaking solution is mixed 1 time during the soaking of petals in dark place.
3. The method for breeding for identifying color rape petals based on spectral features according to claim 1, wherein in step S2, full-band scanning is performed on an ultraviolet-visible spectrophotometer.
4. The method for breeding for identifying color of colored rape petals based on spectral characteristics according to claim 1, wherein in step S4, yellow rape flowers with excellent growth vigor, lodging resistance and disease resistance are selected as male parents for hybridization.
5. The method for breeding for identifying color of colored rape petals based on spectral characteristics according to claim 1, wherein in step S6, seed reserving is performed by bagging or bagging.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145330A (en) * | 2015-06-15 | 2015-12-16 | 浙江省农业科学院 | Breeding method and planting method for colored-flower rape used for creative agriculture |
| CN105766617A (en) * | 2016-03-12 | 2016-07-20 | 临澧县祥云旅游开发有限公司 | Selection method of ornamental red cabbage oilseed rapes |
| US20160278314A1 (en) * | 2015-03-24 | 2016-09-29 | Agrigenetics, Inc. | Canola hybrid clutivar cl2362968h |
| CN107173220A (en) * | 2017-07-18 | 2017-09-19 | 汉中市农业科学研究所（陕西省水稻研究所） | It is a kind of for the breeding method for the purple leaf rape gone sightseeing |
| CN112997878A (en) * | 2021-03-09 | 2021-06-22 | 湖州市农业科技发展中心(湖州市农业科学研究院) | Breeding method of purple rape flowers |
-
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- 2022-11-22 CN CN202211468548.XA patent/CN116114589A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160278314A1 (en) * | 2015-03-24 | 2016-09-29 | Agrigenetics, Inc. | Canola hybrid clutivar cl2362968h |
| CN105145330A (en) * | 2015-06-15 | 2015-12-16 | 浙江省农业科学院 | Breeding method and planting method for colored-flower rape used for creative agriculture |
| CN105766617A (en) * | 2016-03-12 | 2016-07-20 | 临澧县祥云旅游开发有限公司 | Selection method of ornamental red cabbage oilseed rapes |
| CN107173220A (en) * | 2017-07-18 | 2017-09-19 | 汉中市农业科学研究所（陕西省水稻研究所） | It is a kind of for the breeding method for the purple leaf rape gone sightseeing |
| CN112997878A (en) * | 2021-03-09 | 2021-06-22 | 湖州市农业科技发展中心(湖州市农业科学研究院) | Breeding method of purple rape flowers |
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| Title |
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| 肖文芳等: ""不同颜色石斛兰色素成分比较"", 《第四届全国花卉资源、育种、栽培及应用技术交流会论文汇编》, pages 10 - 15 * |
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