CN116099358A - All-electric-driven micro-fluidic dielectrophoresis separation device and method - Google Patents
All-electric-driven micro-fluidic dielectrophoresis separation device and method Download PDFInfo
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- 238000000926 separation method Methods 0.000 title claims abstract description 67
- 238000004720 dielectrophoresis Methods 0.000 title claims abstract description 34
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- 239000000523 sample Substances 0.000 claims abstract description 45
- 239000002245 particle Substances 0.000 claims abstract description 27
- 230000005684 electric field Effects 0.000 claims abstract description 16
- 239000012488 sample solution Substances 0.000 claims abstract description 14
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 97
- 238000003860 storage Methods 0.000 claims description 90
- 239000000243 solution Substances 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 8
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- 239000007853 buffer solution Substances 0.000 claims description 6
- 238000001514 detection method Methods 0.000 abstract description 8
- 229910001338 liquidmetal Inorganic materials 0.000 abstract description 8
- 238000005370 electroosmosis Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
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- 239000004793 Polystyrene Substances 0.000 description 5
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- 239000008367 deionised water Substances 0.000 description 1
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Abstract
The invention relates to the technical field of microfluidic separation, and discloses a full-electric-driven microfluidic dielectrophoresis separation device and a method, wherein the dielectrophoresis separation device comprises a main channel for realizing the separation function of a mixed sample; three branch channel groups for realizing channel fluid driving, sheath flow compression and particle separation functions; realizing a liquid metal electrode channel for forming an uneven electric field; DC power supply for realizing sample injection control and AC power supply with separation function and channel contact electrode. The device realizes continuous driving of sample solution in the main channel by forming hydraulic pressure difference at two ends of the main channel through electroosmosis flow of the three branch channel groups, and realizes separation of mixed particles through uneven electrodes formed by the electrodes. The dielectrophoresis separation device and the dielectrophoresis separation method provided by the invention are very beneficial to realizing the separation of portable mixed samples, thereby promoting the development of POC detection.
Description
Technical Field
The invention relates to the technical field of microfluidic separation, in particular to a full-electric-driven microfluidic dielectrophoresis separation device and method.
Background
Microfluidic chip systems have been increasingly used in various detection fields, such as disease diagnosis, drug analysis, food safety, environmental monitoring, etc., since the twentieth century. The device has the advantages of simple operation, low cost, small volume and small reagent consumption, and has obvious advantages compared with the traditional detection and analysis instrument.
Microfluidic technology has become an important tool for separating nanoparticles with uniform properties due to its precision, versatility and scalability. The present application method of micro-fluidic technology in micro-nano particle separation and enrichment mainly comprises an acoustic flow control technology, a photoelectric flow control technology, a dielectrophoresis technology, a deterministic lateral displacement technology and an inertial micro-fluidic technology, wherein Dielectrophoresis (DEP) phenomenon refers to offset motion of medium particles in a non-uniform electric field due to polarization effect in a micro-fluidic device, and the maneuverability and convenience of the medium particles on the aspect of separating and mixing micro-nano particles have been widely studied.
The existing separation device needs to be externally connected with large-scale driving equipment, the integral size of the equipment is large, the integration of the system is difficult to realize, and the cost is high.
Disclosure of Invention
The invention aims to provide a full-electric-driven micro-fluidic dielectrophoresis separation device and a method, which solve the problems that the existing separation device needs to be externally connected with large-scale driving equipment, is difficult to realize system integration and has high cost.
The invention is realized by the following technical scheme:
an all-electric-driven microfluidic dielectrophoresis separation device comprises a sample liquid storage tank, a first liquid storage tank, a second liquid storage tank, a third liquid storage tank, a main channel, a separation structure, a first direct current power supply, a second direct current power supply and an alternating current power supply;
one end of the main channel is communicated with the sample liquid storage tank, the other end of the main channel is respectively communicated with the second liquid storage tank and the third liquid storage tank through the second branch channel group and the third branch channel group, and one end of the main channel, which is close to the sample liquid storage tank, is communicated with the first liquid storage tank through the first branch channel group;
the first direct current power supply is used for enabling the interface of the main channel and the first branch channel group to form positive pressure; the second direct current power supply is used for enabling the interfaces of the main channel, the second branch channel group and the third branch channel group to form negative pressure;
the separation structure and the alternating current power supply are used for generating an uneven electric field, so that particles in the main channel are subjected to dielectrophoresis forces of different magnitudes to generate different lateral displacements, and then enter the second branch channel group and the third branch channel group respectively.
The dielectrophoresis separation device of the invention forms hydraulic pressure difference at two ends of a main channel through electroosmosis flow of three branch channel groups to realize continuous driving of sample solution in the main channel; the separation of the mixed particles is achieved by means of non-uniform electrodes formed by the electrodes. The system integration can be realized without external large-scale driving equipment, the portable mixed sample separation can be realized, and the problem that the existing separation device is high in cost is solved.
Further, the first reservoir includes a first outlet reservoir and a first inlet reservoir;
a first contact electrode and a second contact electrode are respectively arranged in the first outlet liquid storage pool and the first inlet liquid storage pool, and the first contact electrode and the second contact electrode are respectively connected with the positive electrode and the negative electrode of the first direct current power supply;
the first outlet liquid storage pool and the first inlet liquid storage pool are communicated with the first branch channel group.
Further, the first branch channel group includes a first branch channel and a first annular channel;
one end of the first branch channel is communicated with the main channel, the other end of the first branch channel is communicated with the first annular channel, and the first annular channel is communicated with the first liquid storage tank.
Further, the second reservoir includes a second inlet reservoir and a second outlet reservoir;
a third contact electrode and a fourth contact electrode are respectively arranged in the second inlet liquid storage pool and the second outlet liquid storage pool, and the third contact electrode and the fourth contact electrode are respectively connected with the negative electrode and the positive electrode of the second direct current power supply;
the second inlet liquid storage pool and the second outlet liquid storage pool are communicated with the second branch channel group.
Further, the second branch channel group includes a second branch channel and a second annular channel;
one end of the second branch channel is communicated with the main channel, and the other end of the second branch channel is communicated with the second annular channel; the second annular channel is in communication with a second reservoir.
Further, the third reservoir comprises a third inlet reservoir and a third outlet reservoir;
a fifth contact electrode and a sixth contact electrode are respectively arranged in the third inlet liquid storage tank and the third outlet liquid storage tank; the fifth contact electrode and the sixth contact electrode are respectively connected with the negative electrode and the positive electrode of the two direct current power supplies;
and the third inlet liquid storage pool and the third outlet liquid storage pool are communicated with the third branch channel group.
Further, the third branch channel group includes a third branch channel and a third annular channel;
one end of the third branch channel is communicated with the main channel, the other end of the third branch channel is communicated with the third annular channel, and the third annular channel is communicated with the third liquid storage tank.
Further, the separation structure comprises a long electrode channel and a short electrode channel which are respectively arranged at two sides of the main channel;
a seventh contact electrode and an eighth contact electrode are respectively arranged in the long electrode channel and the short electrode channel; the seventh contact electrode and the eighth contact electrode are respectively connected with two ends of an alternating current power supply.
Further, a blocking microcolumn group is arranged in the long electrode channel and is positioned at the joint of the long electrode channel and the sample injection main channel.
The separation method based on the dielectrophoresis separation device comprises the following steps:
s1, filling the same amount of electrolytic buffer solution in a sample liquid storage tank, a first liquid storage tank, a second liquid storage tank and a third liquid storage tank, so that the sample liquid storage tank, the first liquid storage tank, the second liquid storage tank and the third liquid storage tank have the same liquid level;
s2, switching on a first direct current power supply and a second direct current power supply, enabling the first branch channel solution to move towards the main channel under the action of electroosmosis flow, forming positive pressure at the interface of the main channel and the first branch channel, enabling the second branch channel solution and the third branch channel solution to move back to the main channel due to the fact that polarities are opposite, forming negative pressure at the interfaces of the main channel, the second branch channel and the third branch channel, forming hydraulic pressure difference at two ends of the main channel, enabling the solution in the main channel to flow, switching on the third alternating current power supply, and forming an uneven electric field area;
s3, adding a sample solution into the sample liquid storage tank, and enabling the sample solution to continuously flow into the main channel under the action of a liquid pressure difference;
s4, repeating the step S3 to form continuous sample injection of a sample solution, and when sample particles pass through an uneven electric field area, different sample particles are subjected to dielectrophoresis forces of different magnitudes to generate different lateral displacements, so that the sample particles respectively enter a second branch channel group and a third branch channel group, and microfluidic separation is realized.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. according to the invention, the electric field condition is provided by a simple direct current and alternating current power supply, and on one hand, the continuous sample injection of the sample solution can be driven by electroosmosis induced pressure flow; on the other hand, the non-uniform electric field formed by the contact electrode of the liquid metal channel is taken as the main force to realize the separation of different substances. The system integration can be realized without external large-scale driving equipment, the portable mixed sample separation can be realized, and the problem of high cost of the conventional separation device is solved; and can promote the development of POC detection.
2. The invention can also be combined with a conventional microfluidic detection means to realize qualitative or quantitative detection of substances. The structure is very beneficial to the manufacture of the hand-held detection equipment for mixed samples, thereby promoting the popularization and timeliness development of biochemical detection.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention. In the drawings:
fig. 1 is a block diagram of an all-electrically driven microfluidic dielectrophoresis separation device according to the present invention.
Fig. 2 is a flow chart of an all-electrically driven microfluidic dielectrophoresis separation method provided by the invention.
Fig. 3 is a schematic diagram of numerical simulation modeling for a separation device in an embodiment provided by the invention.
Fig. 4 is a graph of a numerical simulation analysis separation effect in a separation main channel according to an embodiment provided by the present invention.
In the drawings, the reference numerals and corresponding part names:
1. a sample reservoir; 21. a first outlet reservoir; 22. a first inlet reservoir; 23. a second inlet reservoir; 24. a second outlet reservoir; 25. a third inlet reservoir; 26. a third outlet reservoir; 31. a first branch channel; 32. a second branch channel; 33. a third branch channel; 41. a first annular channel; 42. a second annular channel; 43. a third annular channel; 51. a long electrode channel; 52. a short electrode channel; 6. a main channel; 7. blocking the microcolumn group; 81. a first contact electrode; 82. a second contact electrode; 83. a third contact electrode; 84. a fourth contact electrode; 85. a fifth contact electrode; 86. a sixth contact electrode; 87. a seventh contact electrode; 88. an eighth contact electrode; 91. a first DC power supply; 92. a second DC power supply; 93. an alternating current power supply.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
Example 1:
as shown in fig. 1, an all-electrically-driven microfluidic dielectrophoresis separation device comprises a sample liquid storage tank 1, a first liquid storage tank, a second liquid storage tank, a third liquid storage tank, a main channel 6, a separation structure, a first direct current power supply 91, a second direct current power supply 92 and an alternating current power supply 93;
one end of the main channel 6 is communicated with the outlet of the sample liquid storage pool 1, the other end of the main channel is respectively communicated with the second liquid storage pool and the third liquid storage pool through the second branch channel group and the third branch channel group, and one end, close to the sample liquid storage pool 1, of the main channel 6 is communicated with the first liquid storage pool through the first branch channel group;
the first direct current power supply 91 is used for forming positive pressure at the interface of the main channel 6 and the first branch channel group; the second dc power supply 92 is configured to form a negative pressure at the interface between the main channel 6 and the second and third branch channel groups;
the separation structure and ac power supply 93 are used to generate a non-uniform electric field, so that particles in the main channel 6 are subjected to dielectrophoresis forces of different magnitudes, and different lateral displacements are generated, so as to enter the second branch channel group and the third branch channel group respectively.
Wherein the first reservoir comprises a first outlet reservoir 21 and a first inlet reservoir 22;
a first contact electrode 81 and a second contact electrode 82 are respectively arranged in the first outlet liquid storage tank 21 and the first inlet liquid storage tank 22, and the first contact electrode 81 and the second contact electrode 82 are respectively connected with the positive electrode and the negative electrode of the first direct current power supply 91;
the first outlet reservoir 21 and the first inlet reservoir 22 are both in communication with the first set of branch channels.
The first branch passage group includes the first branch passage 31 and the first annular passage 41;
the first branch channel 31 communicates at one end with the main channel 6 and at the other end with a first annular channel 41, said first annular channel 41 communicating with the first outlet reservoir 21 and the first inlet reservoir 22.
Wherein the second reservoir comprises a second inlet reservoir 23 and a second outlet reservoir 24;
a third contact electrode 83 and a fourth contact electrode 84 are respectively arranged in the second inlet liquid storage tank 23 and the second outlet liquid storage tank 24, and the third contact electrode 83 and the fourth contact electrode 84 are respectively connected with the negative electrode and the positive electrode of the second direct current power supply 92;
the second inlet reservoir 23 and the second outlet reservoir 24 are both in communication with the second set of branch channels.
The second branch channel group includes the second branch channel 32 and the second annular channel 42;
one end of the second branch channel 32 is communicated with the main channel 6, and the other end is communicated with the second annular channel 42; the second annular channel 42 communicates with the second inlet reservoir 23 and the second outlet reservoir 24.
Wherein the third reservoir comprises a third inlet reservoir 25 and a third outlet reservoir 26;
a fifth contact electrode 85 and a sixth contact electrode 86 are provided in the third inlet reservoir 25 and the third outlet reservoir 26, respectively; the fifth contact electrode 85 and the sixth contact electrode 86 are respectively connected with the negative electrode and the positive electrode of the two direct current power sources 92;
the third inlet reservoir 25 and the third outlet reservoir 26 are both in communication with the third set of branch channels.
The third branch passage group includes a third branch passage 33 and a third annular passage 43;
the third branch channel 33 has one end communicating with the main channel 6 and the other end communicating with a third annular channel 43, the third annular channel 43 communicating with the third inlet reservoir 25 and the third outlet reservoir 26.
In this embodiment, the main channel 6 and the branch channel group are used to realize the sample driving function, the channel walls of the first annular channel 41, the second annular channel 42 and the third annular channel 43 have electroosmotic sliding characteristics, the buffer solution can be driven to flow in the channels by the loading of the electric field, the flow direction of the buffer solution in the first annular channel 41 is from the first outlet liquid storage tank 21 to the first inlet liquid storage tank 22, and a positive pressure area is formed at the intersection part of the first branch channel 31 and the first annular channel 41; because the polarities of the electrodes are opposite, the intersection part of the second branch channel 32 and the second annular channel 42 and the intersection part of the third branch channel 33 and the third annular channel 43 form a negative pressure area, so that a negative pressure area is formed at the outlet of the sample liquid storage tank 1, if the sample solution to be separated is injected into the sample liquid storage tank, the sample solution can continuously flow into the main channel under the action of negative pressure and is pressed to one side of the main channel by the sheath flow of the first branch channel, and the continuous sample injection function is realized.
Wherein the separation structure comprises a long electrode channel 51 and a short electrode channel 52 which are respectively arranged at two sides of the main channel 6, and a blocking micro-column group 7;
a seventh contact electrode 87 and an eighth contact electrode 88 are provided in the long electrode path 51 and the short electrode path 52, respectively; the seventh contact electrode 87 and the eighth contact electrode 88 are connected to both ends of the ac power supply 93, respectively.
A blocking micro-column group 7 is arranged in the long electrode channel 51, and the blocking micro-column group 7 is positioned at the joint of the long electrode channel 51 and the sample injection main channel 6.
Wherein the long electrode channel 51 and the short electrode channel 52 are used for injecting liquid metal as a seventh contact electrode 87 and an eighth contact electrode 88, and when the liquid metal is injected into the electrode channels, the blocking microcolumn group 7 can effectively prevent the liquid metal from diffusing into the main channel; after the seventh contact electrode 87 and the eighth contact electrode 88 are turned on the third alternating current power source, an uneven electric field is formed in the main channel.
In the present embodiment, the polarities of the first contact electrode 81, the second contact electrode 82, the third contact electrode 83, the fourth contact electrode 84, the fifth contact electrode 85, and the sixth contact electrode 86 each include a positive electrode and a negative electrode; the seventh contact electrode 87 and the eighth contact electrode 88 have no polarity difference. The polarities of the first contact electrode 81, the fourth contact electrode 84 and the sixth contact electrode 86 are all positive, and the polarities of the second contact electrode 82, the third contact electrode 83 and the fifth contact electrode 85 are all negative.
In the embodiment, two direct current power supplies are used for realizing the driving sample injection function, one alternating current power supply is used for realizing the separation of samples, the driving and the separation are realized by the power supplies, and the two direct current power supplies are easy to integrate with a chip, so that the manufacture of a portable instrument is realized; the separation electrode is manufactured into a three-dimensional side electrode by injecting liquid metal, so that the manufacture of the whole chip is simplified, and the three-dimensional side electrode can better realize the separation of sample particles.
Example 2:
as shown in fig. 2, the separation method based on the dielectrophoresis separation device described in embodiment 1 includes the following steps:
s1, filling the same amount of electrolytic buffer solution into a sample liquid storage pool 1, a first liquid storage pool, a second liquid storage pool and a third liquid storage pool, so that the sample liquid storage pool 1, the first liquid storage pool, the second liquid storage pool and the third liquid storage pool have the same liquid level;
s2, switching on a first direct current power supply 91 and a second direct current power supply 92, under the action of electroosmotic flow, enabling the solution in the first branch channel 31 to move towards the main channel 6, forming positive pressure at the interface of the main channel 6 and the first branch channel 31, enabling the solution in the third branch channel 33 of the second branch channel 32 to move back towards the main channel 6, forming negative pressure at the interfaces of the main channel 6, the second branch channel 32 and the third branch channel 33, forming hydraulic pressure difference at two ends of the main channel 6, enabling the solution in the main channel 6 to flow, switching on an alternating current power supply 93, and forming an uneven electric field area;
different particles can generate different lateral displacements in an uneven electric field to be separated;
s3, adding a sample solution into the sample liquid storage tank 1, and enabling the sample solution to continuously flow into the main channel 6 under the action of a liquid pressure difference;
s4, repeating the step S3 to form continuous sample injection of a sample solution, and when sample particles pass through an uneven electric field area, different sample particles are subjected to dielectrophoresis forces of different magnitudes to generate different lateral displacements, so that the sample particles respectively enter the second branch channel 32 and the third branch channel 33, and microfluidic separation is realized.
Example 3:
in order to study the effect of achieving separation based on the principle of the separation device shown in embodiment 1, simulation analysis was performed on the structure in this embodiment; first, assuming that two substances to be separated in the structure are polystyrene particles, setting particle properties in a simulation model according to properties of the polystyrene particles.
In the simulation model, since the liquid reservoir has a much larger size than the channel, the effect on the distribution of the fluid flow field is negligible, and therefore, the liquid reservoir is ignored in the simulation model, the long electrode channel and the short electrode channel have the effect of injecting liquid metal as electrodes, and the distribution of the fluid flow field is not affected, but since the side wall formed by the injected liquid metal is lower than the channel wall, the junction of the main channel 6 and the long electrode channel 51 and the short electrode channel 52 forms long grooves and short grooves. The corresponding simulation model of fig. 1 is shown in fig. 3. Assume that the two polystyrene particles to be separated have a density of 1050kg/m 3 The diameters are respectively 10 μm and 5 μm, the relative dielectric constant of the particles is 2.55, the conductivity of the polystyrene particles of 10 μm is 0.0008S/m, the conductivity of the polystyrene particles of 5 μm is 0.0004S/m, the buffer solution is deionized water, the voltage of the two direct current power supplies is 100V, the voltage of the alternating current power supply is 5V, and the frequency is 100Khz.
For the separation model in fig. 3, the dimensions of the main channel 6 were defined as 1400 (μm) x 70 (μm), the length of the long electrode at the interface with the main channel 6 was 500 (μm), the length of the short electrode at the interface with the main channel 6 was 50 (μm), the dimensions of the first, second and third branch channels 31, 32 and 33 were 3500 (μm) x 70 (μm), the dimensions of the first, second and third annular channels 41, 42 and 43 were 10000 (μm) x 50 (μm), wherein the horizontal channel length 2000 (μm) and the vertical channel length 3000 (μm), and the channel dimensions connecting the two vertical channels and the two inlets of the reservoir were 1130 (μm) x 70 (μm) (here, the horizontal and vertical are set as vertical with respect to the branch channels). The particle separation effect in the main channel is simulated over a period of time as shown in figure 4.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
It should be noted that the structures, proportions, sizes, etc. shown in the drawings attached to the present specification are for understanding and reading only by those skilled in the art, and are not intended to limit the scope of the invention, so that any structural modifications, proportional changes, or size adjustments should fall within the scope of the invention without affecting the efficacy and achievement of the present invention. Also, the terms such as "upper", "lower", "left", "right", "middle", and the like are used herein for descriptive purposes only and are not intended to limit the scope of the invention for which the invention may be practiced or for which the relative relationships may be altered or modified without materially altering the technical context.
Claims (10)
1. The all-electric-driven microfluidic dielectrophoresis separation device is characterized by comprising a sample liquid storage tank (1), a first liquid storage tank, a second liquid storage tank, a third liquid storage tank, a main channel (6), a separation structure, a first direct current power supply (91), a second direct current power supply (92) and an alternating current power supply (93);
one end of the main channel (6) is communicated with the sample liquid storage pool (1), the other end of the main channel is respectively communicated with the second liquid storage pool and the third liquid storage pool through the second branch channel group and the third branch channel group, and one end, close to the sample liquid storage pool (1), of the main channel (6) is communicated with the first liquid storage pool through the first branch channel group;
the first direct current power supply (91) is used for enabling the interface of the main channel (6) and the first branch channel group to form positive pressure; the second direct current power supply (92) is used for enabling the interfaces of the main channel (6) and the second branch channel group and the third branch channel group to form negative pressure;
the separation structure and the alternating current power supply (93) are used for generating an uneven electric field, so that particles in the main channel (6) are subjected to dielectrophoresis forces of different magnitudes to generate different lateral displacements, and then enter the second branch channel group and the third branch channel group respectively.
2. An all-electrically driven microfluidic dielectrophoresis separation device according to claim 1, wherein the first reservoir comprises a first outlet reservoir (21) and a first inlet reservoir (22);
a first contact electrode (81) and a second contact electrode (82) are respectively arranged in the first outlet liquid storage tank (21) and the first inlet liquid storage tank (22), and the first contact electrode (81) and the second contact electrode (82) are respectively connected with the positive electrode and the negative electrode of the first direct current power supply (91);
the first outlet liquid storage tank (21) and the first inlet liquid storage tank (22) are communicated with the first branch channel group.
3. An all-electrically driven microfluidic dielectrophoresis separation device according to claim 1, wherein the first set of branch channels comprises a first branch channel (31) and a first annular channel (41);
one end of the first branch channel (31) is communicated with the main channel (6), the other end of the first branch channel is communicated with the first annular channel (41), and the first annular channel (41) is communicated with the first liquid storage tank.
4. An all-electrically driven microfluidic dielectrophoresis separation device according to claim 1, wherein the second reservoir comprises a second inlet reservoir (23) and a second outlet reservoir (24);
a third contact electrode (83) and a fourth contact electrode (84) are respectively arranged in the second inlet liquid storage tank (23) and the second outlet liquid storage tank (24), and the third contact electrode (83) and the fourth contact electrode (84) are respectively connected with the negative electrode and the positive electrode of a second direct current power supply (92);
the second inlet liquid storage tank (23) and the second outlet liquid storage tank (24) are communicated with the second branch channel group.
5. An all-electrically-driven microfluidic dielectrophoresis separation device according to claim 1, wherein the second set of branch channels comprises a second branch channel (32) and a second annular channel (42);
one end of the second branch channel (32) is communicated with the main channel (6), and the other end of the second branch channel is communicated with the second annular channel (42); the second annular channel (42) communicates with a second reservoir.
6. An all-electrically driven microfluidic dielectrophoresis separation device according to claim 1, wherein the third reservoir comprises a third inlet reservoir (25) and a third outlet reservoir (26);
a fifth contact electrode (85) and a sixth contact electrode (86) are respectively arranged in the third inlet liquid storage tank (25) and the third outlet liquid storage tank (26); the fifth contact electrode (85) and the sixth contact electrode (86) are respectively connected with the negative electrode and the positive electrode of the two direct current power supplies (92);
the third inlet liquid storage tank (25) and the third outlet liquid storage tank (26) are communicated with the third branch channel group.
7. An all-electrically-driven microfluidic dielectrophoresis separation device according to claim 1, wherein the third set of branch channels comprises a third branch channel (33) and a third annular channel (43);
one end of the third branch channel (33) is communicated with the main channel (6), the other end of the third branch channel is communicated with the third annular channel (43), and the third annular channel (43) is communicated with the third liquid storage tank.
8. An all-electrically driven microfluidic dielectrophoresis separation device according to claim 1, wherein the separation structure comprises long electrode channels (51) and short electrode channels (52) arranged on either side of the main channel (6);
a seventh contact electrode (87) and an eighth contact electrode (88) are respectively arranged in the long electrode channel (51) and the short electrode channel (52); the seventh contact electrode (87) and the eighth contact electrode (88) are respectively connected with two ends of an alternating current power supply (93).
9. The all-electrically-driven microfluidic dielectrophoresis separation device according to claim 8, wherein a blocking microcolumn group (7) is arranged in the long electrode channel (51), and the blocking microcolumn group (7) is positioned at the joint of the long electrode channel (51) and the sample introduction main channel (6).
10. A separation method based on a microfluidic dielectrophoresis separation device according to any one of claims 1 to 9, comprising the steps of:
s1, filling the same amount of electrolytic buffer solution into a sample liquid storage pool (1), a first liquid storage pool, a second liquid storage pool and a third liquid storage pool, so that the sample liquid storage pool (1), the first liquid storage pool, the second liquid storage pool and the third liquid storage pool have the same liquid level;
s2, switching on a first direct current power supply (91) and a second direct current power supply (92), and forming positive pressure at the interface of the main channel (6) and the first branch channel group; negative pressure is formed at the interfaces of the main channel (6) and the second branch channel group and the third branch channel group; the two ends of the main channel (6) form a hydraulic pressure difference, the solution in the main channel (6) flows, and an alternating current power supply (93) is connected to form an uneven electric field area;
s3, adding a sample solution into the sample liquid storage tank (1) to enable the sample solution to continuously flow into the main channel (6) under the action of a hydraulic pressure difference;
s4, repeating the step S3 to form continuous sample injection of a sample solution, and when sample particles pass through an uneven electric field area, different sample particles are subjected to dielectrophoresis forces of different magnitudes to generate different lateral displacements, so that the sample particles respectively enter a second branch channel group and a third branch channel group, and microfluidic separation is realized.
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