CN116096877B - Ii类ii型crispr系统 - Google Patents

Ii类ii型crispr系统

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Publication number
CN116096877B
CN116096877B CN202180039435.5A CN202180039435A CN116096877B CN 116096877 B CN116096877 B CN 116096877B CN 202180039435 A CN202180039435 A CN 202180039435A CN 116096877 B CN116096877 B CN 116096877B
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CN
China
Prior art keywords
sequence
endonuclease
engineered
seq
domain
Prior art date
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CN202180039435.5A
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English (en)
Chinese (zh)
Other versions
CN116096877A (zh
Inventor
布莱恩·托马斯
克利斯多佛·布朗
奥德拉·德沃托
克里斯蒂娜·布特弗尔德
利萨·亚历山大
丹妮拉·S·A·戈尔茨曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Macrogenomics
Macrogenomics Therapy Co
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Macrogenomics
Macrogenomics Therapy Co
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Publication of CN116096877A publication Critical patent/CN116096877A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Confectionery (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)
CN202180039435.5A 2020-03-31 2021-03-30 Ii类ii型crispr系统 Active CN116096877B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063003159P 2020-03-31 2020-03-31
US63/003,159 2020-03-31
US202063116149P 2020-11-19 2020-11-19
US63/116,149 2020-11-19
PCT/US2021/024945 WO2021202568A1 (en) 2020-03-31 2021-03-30 Class ii, type ii crispr systems

Publications (2)

Publication Number Publication Date
CN116096877A CN116096877A (zh) 2023-05-09
CN116096877B true CN116096877B (zh) 2026-04-28

Family

ID=77929138

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180039435.5A Active CN116096877B (zh) 2020-03-31 2021-03-30 Ii类ii型crispr系统

Country Status (11)

Country Link
US (2) US11946039B2 (https=)
EP (2) EP4632066A3 (https=)
JP (2) JP7546689B2 (https=)
KR (2) KR102794727B1 (https=)
CN (1) CN116096877B (https=)
AU (1) AU2021248800A1 (https=)
CA (1) CA3177051A1 (https=)
ES (1) ES3055163T3 (https=)
GB (1) GB2608292B (https=)
MX (1) MX2022012110A (https=)
WO (1) WO2021202568A1 (https=)

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WO2021146641A1 (en) * 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
WO2021202568A1 (en) 2020-03-31 2021-10-07 Metagenomi Ip Technologies, Llc Class ii, type ii crispr systems
JP2023540797A (ja) * 2020-09-11 2023-09-26 メタゲノミ，インコーポレイテッド 塩基編集酵素
AU2022335499A1 (en) 2021-08-27 2024-02-22 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
WO2023081855A1 (en) 2021-11-05 2023-05-11 Metagenomi, Inc. Base editing enzymes
EP4437095A1 (en) * 2021-11-24 2024-10-02 Metagenomi, Inc. Endonuclease systems
AU2022396533A1 (en) 2021-11-24 2024-05-02 Metagenomi Therapeutics, Inc. Endonuclease systems
ES2970263B2 (es) * 2022-10-21 2024-10-11 Univ Alicante PROTEINA ENDONUCLEASA Cas9 Y SISTEMA CRISPR-Cas ASOCIADO
AU2024236558A1 (en) 2023-03-15 2025-10-09 Renagade Therapeutics Management Inc. Delivery of gene editing systems and methods of use thereof
WO2024206759A1 (en) * 2023-03-31 2024-10-03 Arbor Biotechnologies, Inc. Crispr nuclease polypeptides and gene editing systems comprising such
CN121241133A (zh) * 2023-03-31 2025-12-30 阿伯生物技术公司 Crispr核酸酶多肽和包含此类crispr核酸酶多肽的基因编辑系统
WO2024206825A2 (en) * 2023-03-31 2024-10-03 Aera Therapeutics, Inc. Modified iscb nucleases, modified guide rnas, and uses thereof
WO2024233366A2 (en) * 2023-05-05 2024-11-14 Aera Therapeutics, Inc. Modified cas9-iid nucleases and uses thereof
EP4716752A2 (en) * 2023-05-23 2026-04-01 Metagenomi, Inc. Endonuclease systems
WO2025007044A1 (en) * 2023-06-28 2025-01-02 The University Of Chicago Methods and compositions comprising iscb variants
AU2024335327A1 (en) 2023-09-01 2026-03-26 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025054425A1 (en) * 2023-09-08 2025-03-13 Arbor Biotechnologies, Inc. Reverse transcription-mediated gene editing systems and uses thereof
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

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Also Published As

Publication number Publication date
MX2022012110A (es) 2022-10-18
KR102794727B1 (ko) 2025-04-11
JP7546689B2 (ja) 2024-09-06
KR20220161383A (ko) 2022-12-06
CN116096877A (zh) 2023-05-09
US11946039B2 (en) 2024-04-02
KR20250051161A (ko) 2025-04-16
EP4127156B1 (en) 2025-09-10
ES3055163T3 (en) 2026-02-10
JP7785139B2 (ja) 2025-12-12
WO2021202568A1 (en) 2021-10-07
US20230051396A1 (en) 2023-02-16
JP2024178181A (ja) 2024-12-24
EP4632066A2 (en) 2025-10-15
AU2021248800A1 (en) 2022-10-13
GB202211839D0 (en) 2022-09-28
EP4127156C0 (en) 2025-09-10
GB2608292B (en) 2024-02-14
EP4127156A4 (en) 2024-03-27
GB2608292A (en) 2022-12-28
EP4127156A1 (en) 2023-02-08
CA3177051A1 (en) 2021-10-07
JP2023519953A (ja) 2023-05-15
EP4632066A3 (en) 2025-12-24
US20240309356A1 (en) 2024-09-19

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