CN116076755A - Preparation method of grease microcapsule with brown fat activating function - Google Patents
Preparation method of grease microcapsule with brown fat activating function Download PDFInfo
- Publication number
- CN116076755A CN116076755A CN202310331009.XA CN202310331009A CN116076755A CN 116076755 A CN116076755 A CN 116076755A CN 202310331009 A CN202310331009 A CN 202310331009A CN 116076755 A CN116076755 A CN 116076755A
- Authority
- CN
- China
- Prior art keywords
- microcapsule
- hairtail
- brown fat
- oil
- offal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003094 microcapsule Substances 0.000 title claims abstract description 74
- 210000003486 adipose tissue brown Anatomy 0.000 title claims abstract description 45
- 239000004519 grease Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 230000003213 activating effect Effects 0.000 title claims abstract description 17
- 235000019198 oils Nutrition 0.000 claims abstract description 51
- 241001125843 Trichiuridae Species 0.000 claims abstract description 42
- 235000021323 fish oil Nutrition 0.000 claims abstract description 42
- URAYPUMNDPQOKB-UHFFFAOYSA-N triacetin Chemical compound CC(=O)OCC(OC(C)=O)COC(C)=O URAYPUMNDPQOKB-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 13
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 13
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 13
- 239000001087 glyceryl triacetate Substances 0.000 claims abstract description 11
- 235000013773 glyceryl triacetate Nutrition 0.000 claims abstract description 11
- 229960002622 triacetin Drugs 0.000 claims abstract description 11
- 229920002472 Starch Polymers 0.000 claims abstract description 8
- 238000000265 homogenisation Methods 0.000 claims abstract description 8
- KCYQMQGPYWZZNJ-BQYQJAHWSA-N hydron;2-[(e)-oct-1-enyl]butanedioate Chemical compound CCCCCC\C=C\C(C(O)=O)CC(O)=O KCYQMQGPYWZZNJ-BQYQJAHWSA-N 0.000 claims abstract description 8
- 239000008107 starch Substances 0.000 claims abstract description 8
- 235000019698 starch Nutrition 0.000 claims abstract description 8
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 239000006185 dispersion Substances 0.000 claims abstract description 6
- 239000011162 core material Substances 0.000 claims abstract 3
- 239000012071 phase Substances 0.000 claims description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 150000002632 lipids Chemical class 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 239000000839 emulsion Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 229940075507 glyceryl monostearate Drugs 0.000 claims description 4
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- -1 sucrose ester Chemical class 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 239000008346 aqueous phase Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 208000024172 Cardiovascular disease Diseases 0.000 claims description 2
- 208000026106 cerebrovascular disease Diseases 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 230000002526 effect on cardiovascular system Effects 0.000 claims description 2
- 235000019441 ethanol Nutrition 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims 2
- 238000009882 destearinating Methods 0.000 claims 1
- 238000007670 refining Methods 0.000 claims 1
- 238000002390 rotary evaporation Methods 0.000 claims 1
- 241000699670 Mus sp. Species 0.000 abstract description 46
- 208000008589 Obesity Diseases 0.000 abstract description 14
- 235000020824 obesity Nutrition 0.000 abstract description 14
- 230000004913 activation Effects 0.000 abstract description 8
- 230000004069 differentiation Effects 0.000 abstract description 8
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract description 8
- 150000004670 unsaturated fatty acids Chemical class 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000020169 heat generation Effects 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 20
- 239000003925 fat Substances 0.000 description 15
- 108090000623 proteins and genes Proteins 0.000 description 15
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 210000001593 brown adipocyte Anatomy 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 6
- 235000019197 fats Nutrition 0.000 description 6
- 229930195729 fatty acid Natural products 0.000 description 6
- 239000000194 fatty acid Substances 0.000 description 6
- 150000004665 fatty acids Chemical class 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 5
- 230000019491 signal transduction Effects 0.000 description 5
- 238000000967 suction filtration Methods 0.000 description 5
- 102000003728 Peroxisome Proliferator-Activated Receptors Human genes 0.000 description 4
- 108090000029 Peroxisome Proliferator-Activated Receptors Proteins 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 101150022052 UCP1 gene Proteins 0.000 description 4
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 4
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 4
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 4
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 4
- 238000010201 enrichment analysis Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 150000003626 triacylglycerols Chemical class 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108010023302 HDL Cholesterol Proteins 0.000 description 3
- 108010028554 LDL Cholesterol Proteins 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 3
- 230000002496 gastric effect Effects 0.000 description 3
- 238000003125 immunofluorescent labeling Methods 0.000 description 3
- 238000011532 immunohistochemical staining Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000003827 upregulation Effects 0.000 description 3
- 210000000636 white adipocyte Anatomy 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 101100154912 Mus musculus Tyrp1 gene Proteins 0.000 description 2
- 101100403108 Schizosaccharomyces pombe (strain 972 / ATCC 24843) mud1 gene Proteins 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229940090949 docosahexaenoic acid Drugs 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000037356 lipid metabolism Effects 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000016670 prohibitin Human genes 0.000 description 2
- 108010028138 prohibitin Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000035924 thermogenesis Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 1
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 1
- 206010000117 Abnormal behaviour Diseases 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 101150054111 Aspg gene Proteins 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000032928 Dyslipidaemia Diseases 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 108091006027 G proteins Proteins 0.000 description 1
- 102000030782 GTP binding Human genes 0.000 description 1
- 108091000058 GTP-Binding Proteins 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 208000017170 Lipid metabolism disease Diseases 0.000 description 1
- 108010050258 Mitochondrial Uncoupling Proteins Proteins 0.000 description 1
- 102000015494 Mitochondrial Uncoupling Proteins Human genes 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000003399 chemotactic effect Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000006020 chronic inflammation Effects 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 230000027288 circadian rhythm Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003777 experimental drug Substances 0.000 description 1
- 230000004129 fatty acid metabolism Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 235000020978 long-chain polyunsaturated fatty acids Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- CASUWPDYGGAUQV-UHFFFAOYSA-M potassium;methanol;hydroxide Chemical compound [OH-].[K+].OC CASUWPDYGGAUQV-UHFFFAOYSA-M 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000013441 quality evaluation Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000476 thermogenic effect Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000011222 transcriptome analysis Methods 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 235000018648 unbalanced nutrition Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/20—Fish extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a preparation method of a grease microcapsule with a brown fat activating function. The invention adopts maltodextrin and octenyl succinic acid esterified starch as wall materials, and takes hairtail leftover refined fish oil and triacetin as core materials, and the oil microcapsule powder product with the brown fat activating function is obtained through high-speed dispersion, homogenization and freeze drying. The grease microcapsule prepared by the method is rich in unsaturated fatty acid, can promote brown fat differentiation and heat generation, activate brown fat activation, and further remarkably reduce the weight of mice, thereby playing a role in preventing and treating obesity and related diseases.
Description
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a preparation method of grease microcapsule powder with a brown fat activating function.
Background
Obesity is one of the major public health problems worldwide, and the pathogenesis is complex, mainly manifested as excessive fat accumulation. Obesity can lead to disorders of body glycolipid metabolism, chronic inflammation, and impaired and disturbed immune systems. In recent years, along with improvement of material life and change of dietary structure, high energy and little exercise lead to rapid increase of prevalence rate of obesity in China, and incidence rate of related diseases such as fatty liver, type 2 diabetes, dyslipidemia, coronary heart disease and the like is also rapidly increasing. The irrational diet structure and unbalanced nutrition metabolism are main causes of obesity, so that reasonable adjustment of diet structure and effective control of energy intake are necessary measures for preventing and treating obesity, and active exploration of new diet guidance and nutrition intervention strategies is an important scientific problem in the field of obesity prevention and control.
Recent researches of scholars show that the activation and differentiation of brown adipocytes can play an important role in improving obesity, fat cells in a human body can be divided into two types, namely brown adipocytes and white adipocytes, and the brown adipocytes are good at storing energy, different from the white adipocytes, and are good at utilizing and consuming energy, have more mitochondria than the white adipocytes, are smaller in lipid droplets, can activate a large amount of expressed uncoupling protein 1 (Ucp 1) to perform energy consumption and heat generation effects after being stimulated, and are one of targets for treating metabolic diseases such as obesity and diabetes. Therefore, how to induce brown fat activation in vivo to consume more energy is a key to treat obesity and related diseases.
Research shows that deep sea fish oil is rich in polyunsaturated fatty acids and its unsaturated fatty acid content is far superior to that of fresh water fish oil. Polyunsaturated fatty acids (PUFAs) are fatty acids having two or more double bonds in the carbon chain, and are important components in maintaining cellular structure and function. Among them, n-3 long chain polyunsaturated fatty acids represented by eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have the ability to activate brown fat, can reduce fat accumulation of brown fat cells and promote thermogenesis by activating expression of Ucp1 to prevent obesity. The triacetin is a product obtained by esterifying acetic acid and glycerol, acetic acid obtained by hydrolyzing the triacetin by lipase can directly enter small intestine epithelial cells to be rapidly absorbed by organisms, and the acetic acid has important effects in regulating energy steady state, regulating blood sugar and improving obesity. The hairtail is a common and widely distributed deep sea fish, is rich in protein, unsaturated fatty acid and lipid, has rich nutritional value and medicinal value, and has great potential for being developed into a dietary supplement or a nutritional health food with the effects of losing weight and reducing blood fat. However, at present, no microcapsule product related to weight and lipid reduction by taking hairtail oil as a raw material exists in the market.
Disclosure of Invention
The invention aims to provide a preparation method of a grease microcapsule with a brown fat activating function. The prepared oil microcapsule has obvious effects of regulating weight and blood sugar, and can activate brown fat.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a process for preparing the oil microcapsule with the function of activating brown fat includes such steps as preparing the refined fish oil from the leftover of hairtail and glyceryl triacetate, preparing the wall material from maltodextrin and octenyl succinic acid esterified starch, high-speed dispersing, homogenizing, and freeze drying.
The specific operation comprises the following steps:
(1) Preparation of aqueous phase
A. Adding maltodextrin into warm water to dissolve until the maltodextrin is completely transparent;
B. adding octenyl succinic acid esterified starch into maltodextrin solution according to the mass ratio of octenyl succinic acid esterified starch to maltodextrin of 2:1, and stirring uniformly to prepare a water phase.
(2) Preparation of the oil phase
A. Removing impurities from fresh hairtail scraps, cleaning, homogenizing, and preserving at-18 ℃ in a dark place;
B. adding proper amount of anhydrous sodium sulfate into the hairtail leftover homogenate, and standing overnight. Then adding absolute ethyl alcohol into hairtail offal in a ratio of 1:6g/mL, uniformly mixing, carrying out microwave for 10min at 600W and 75 ℃ in a microwave extraction instrument, carrying out suction filtration to remove hairtail offal residues, collecting filtrate, and carrying out rotary steaming to obtain hairtail offal crude fish oil;
C. heating hairtail offal crude fish oil to 65deg.C, continuously stirring, adding phosphoric acid solution (85% w/w) accounting for 1% (v/v) of the substrate, slowly stirring for 1min, centrifuging at 8000 r/min for 15min, layering, and collecting upper oil phase. Heating degummed fish oil to 65deg.C, adding NaOH solution (4%, w/w) accounting for 2.5% (v/v) of substrate, stirring for 5min, centrifuging at 8000 r/min for 15min, layering, collecting upper layer oil, washing at 65deg.C for 2 times with 1/3 oil (v/v) NaCl solution (5%, w/w), washing with 75deg.C hot water, layering with a separating funnel, and collecting upper layer oil phase. Adding activated clay accounting for 3% (w/w) of the substrate into the deacidified fish oil, stirring and decoloring for 30 min at 80 ℃, and rapidly filtering under hot pressure (air pressure is 0.04 MPa) to obtain decolored fish oil. Placing decolorized fish oil in a refrigerator at 8deg.C for 2 days, centrifuging at 8deg.C for 30 min at 8000 r/min, collecting upper layer oil, placing at 0deg.C for 1d, centrifuging at 0deg.C for 30 min at 8000 r/min, and collecting the supernatant.
D. Mixing hairtail offal refined fish oil and glyceryl triacetate according to a mass ratio of 2:1, then adding an emulsifying agent (the mass ratio of sucrose ester to glyceryl monostearate is 9:1) (the using amount of the emulsifying agent is 1% of the total mass of the hairtail offal refined fish oil and the glyceryl triacetate), and then pouring a small amount of water for heating and dissolving to obtain an oil phase.
(3) Preparation of fish oil microcapsule
A. Adding the oil phase into the water phase (the mass ratio of the oil phase to the water phase is 1:2), adding water according to the total solid content of 40 percent wt percent, mixing the oil phase and the water phase, and obtaining stable emulsion after high-speed dispersion homogenization treatment (homogenization time is 1min and homogenization rotation speed is 10000 r/min);
B. pre-freezing the emulsion at-20deg.C for 8h, freezing to obtain ice solid, and freeze drying (-50deg.C, 50 Pa) for 24 hr to obtain oil microcapsule powder.
Application: the grease microcapsule is applied to the preparation of weight-losing and lipid-lowering health care products or medicaments for preventing/relieving cardiovascular and cerebrovascular diseases.
The invention has the beneficial effects that:
the hairtail offal is taken as a raw material, and the hairtail offal oil rich in unsaturated fatty acid is obtained by extracting by a microwave-assisted ethanol method, wherein the unsaturated fatty acid content is about 63.03%, the polyunsaturated fatty acid content is about 29.71%, and the polyunsaturated fatty acid content is mainly EPA and DHA, and is about 6.57% and 18.75% respectively; meanwhile, the oil is embedded by utilizing a microcapsule preparation technology, and the oil microcapsule rich in unsaturated fatty acid is prepared by a freeze-drying technology, so that the loss rate of the oil is effectively inhibited, the embedding rate reaches more than 95%, the oxidation of active ingredients is avoided, the processing and storage stability is improved, and the obtained product is convenient to store, package and transport. The grease microcapsule can obviously reduce the weight and the blood sugar level; can obviously promote the differentiation and heat production of brown fat cells, activate the activation of brown fat to prevent obesity, has no obvious toxic or side effect, and has safe and reliable source.
Drawings
FIG. 1 effect of lipid microcapsules on mouse body weight;
FIG. 2 results of glucose tolerance experiments in mice of different treatment groups after cold exposure;
FIG. 3 effect of lipid microcapsules on lipid levels in mice (TG, TCHO, HDL-C, LDL-C);
FIG. 4 effect of lipid microcapsules on carbon dioxide production during respiration of mice;
FIG. 5 effect of lipid microcapsules on breath entropy of mice;
FIG. 6 effect of lipid microcapsules on immunohistochemical staining results of brown adipose tissue sections of mice;
FIG. 7 effect of lipid microcapsules on immunofluorescent staining results of brown adipose tissue sections of mice, wherein (A) immunofluorescent staining of brown adipose tissue of mice of different treatment groups, (B) effect of lipid microcapsules on average fluorescence intensity of Prohibitin in brown adipose tissue sections of mice;
FIG. 8 results of GO functional enrichment analysis of mouse brown fat differential genes from the high lipidic vs microcapsule group;
FIG. 9 results of KEGG enrichment analysis of mouse brown fat differential genes for blank vs microcapsule group;
"#" indicates p <0.05, and "#" indicates p <0.01.
Detailed Description
The technical scheme of the present invention will be described in further detail below with reference to practical embodiments, but the present invention is not limited thereto and is not to be construed as the full scope of the present invention.
Example 1
Preparation of grease microcapsules:
(1) Preparation of aqueous phase
1. Adding warm water into maltodextrin at a ratio of 1:5 g/mL, and dissolving the maltodextrin until the maltodextrin is completely transparent;
2. adding octenyl succinic acid esterified starch into maltodextrin solution according to the mass ratio of octenyl succinic acid esterified starch to maltodextrin of 2:1, and stirring uniformly to prepare a water phase.
(2) Preparation of the oil phase
1. Removing impurities from fresh hairtail scraps, cleaning, homogenizing, and preserving at-18 ℃ in a dark place;
2. adding anhydrous sodium sulfate into hairtail leftover homogenate according to the mass ratio of 2:1, and standing overnight. Then adding absolute ethyl alcohol into hairtail offal in a ratio of 1:6g/mL, uniformly mixing, carrying out microwave for 10min at 600W and 75 ℃ in a microwave extraction instrument, carrying out suction filtration to remove hairtail offal residues, collecting filtrate, and carrying out rotary steaming to obtain hairtail offal crude fish oil;
3. heating hairtail offal crude fish oil to 65deg.C, continuously stirring, adding phosphoric acid solution (85% w/w) accounting for 1% (v/v) of the substrate, slowly stirring for 1min, centrifuging at 8000 r/min for 15min, layering, and collecting upper oil phase. Heating degummed fish oil to 65deg.C, adding NaOH solution (4%, w/w) accounting for 2.5% (v/v) of substrate, stirring for 5min, centrifuging at 8000 r/min for 15min, layering, collecting upper layer oil, washing at 65deg.C for 2 times with 1/3 oil (v/v) NaCl solution (5%, w/w), washing with 75deg.C hot water, layering with a separating funnel, and collecting upper layer oil phase. Adding activated clay accounting for 3% (w/w) of the substrate into the deacidified fish oil, stirring and decoloring for 30 min at 80 ℃, and rapidly filtering under hot pressure (air pressure is 0.04 MPa) to obtain decolored fish oil. Placing decolorized fish oil in a refrigerator at 8deg.C for 2 days, centrifuging at 8deg.C for 30 min at 8000 r/min, collecting upper layer oil, placing at 0deg.C for 1d, centrifuging at 0deg.C for 30 min at 8000 r/min, and collecting the supernatant as hairtail offal refined fish oil;
4. mixing hairtail offal refined fish oil and glyceryl triacetate according to a mass ratio of 2:1, then adding an emulsifying agent (the mass ratio of sucrose ester to glyceryl monostearate is 9:1) (the using amount of the emulsifying agent is 1% of the total mass of the hairtail offal refined fish oil and the glyceryl triacetate), and then pouring a small amount of water for heating and dissolving to obtain an oil phase.
(3) Preparation of fish oil microcapsule
1. Adding the oil phase into the water phase (the mass ratio of the oil phase to the water phase is 1:2), adding water according to the total solid content of 40wt%, mixing the oil phase and the water phase, and carrying out high-speed dispersion homogenization treatment (homogenization time is 1min, and homogenization rotation speed is 10000 r/min) to obtain stable emulsion;
2. pre-freezing the emulsion at-20deg.C for 8 hr, freezing to obtain ice solid, and freeze drying (-50deg.C, 50 Pa) for 24 hr to obtain oil microcapsule powder.
(4) Determination of physical and chemical indexes of hairtail leftover refined fish oil
The water content, acid value, iodine value and peroxide value of the hairtail offal refined fish oil are measured respectively with reference to national standards GB/T5528-2008, GB/T5530-2005, CB/T5532-2008 and GB/T5538-2005.
(5) Determination of fatty acid composition of hairtail offal refined fish oil
1. Taking 3-4 drops of fish oil into a 10mL test tube with a plug, adding 2 mL petroleum ether, slightly vibrating to fully dissolve the grease, adding 2 mL of 0.4 mol/L potassium hydroxide-methanol solution, shaking uniformly, and standing at room temperature for 20min. Adding a proper amount of deionized water, and taking supernatant for gas chromatography-mass spectrometry analysis after the organic layer floats upwards;
2. chromatographic conditions: chromatographic column: FFAP quartz capillary column (30 m 0.25mm 0.25 μm); a detector FID; the sample inlet and the detector are both at 250 ℃; chromatographic column temperature program: keeping at 190 ℃ for 15min, and heating to 230 ℃ at 5 ℃/min; carrier gas: nitrogen gas;
3. the mass spectrum conditional ion source is an EI source, and the electron energy is 70eV; the ion source temperature is 230 ℃; the temperature of the four-stage rod is 150 ℃; multiplier voltage 1388V; emission current 34.6 μa; scanning the range of 30-500 amu; scanning speed: 500 amu/s. The sample injection amount is 1 mu L by adopting a full scanning mode.
(6) Determination of physicochemical index of oil microcapsule
1. Surface oil content: 2g of fish oil microcapsule is taken and placed in a conical flask, 20 mL petroleum ether is added to dissolve a sample, the sample is poured into a suction filtration device, 10mL petroleum ether is added to wash the sample in the residual conical flask in a suction filtration device in batches, filtrate is poured into an evaporation dish, suction filtration is repeated for three times, and the constant weight is dried after the reagent is volatilized;
2. water content: determination was performed with reference to GB/T5009.3-2016, moisture content= (mass before sample drying-mass after sample constant weight)/mass before sample drying x 100%;
3. embedding rate: total oil content was determined with reference to GB/T5009.6-2016, embedding rate = (total oil-surface oil)/total oil x 100%;
4. oil carrying amount: the oil carrying amount was calculated from the above index, and the oil carrying amount=total oil/(microcapsule× (1-moisture content)) ×100%.
Experimental results:
1. the hairtail offal refined fish oil prepared by the method has the moisture content and volatile matter content of 0.17%, the acid value of 0.42mg/g, the iodine value of 142.67g/100g and the peroxide value of 0.47meq/kg, and the physicochemical indexes all meet the standard of the refined fish oil; the fatty acid component detection results of the hairtail offal refined fish oil are shown in table 1, and 16 kinds of fatty acids are identified in the hairtail offal refined fish oil, wherein the unsaturated fatty acid content is about 63.03%, and the polyunsaturated fatty acid content accounts for 29.71%, mainly EPA and DHA, and the unsaturated fatty acid content is 6.57% and 18.75% respectively.
2. The oil microcapsule obtained by the preparation method has the advantages of no caking in appearance color, no impurities, coarse white powder, water content of 4.44%, oil loading of 30.65%, surface oil content of 0.96% and embedding rate of 96.63%.
The microcapsule prepared by the method has high oil content, no caking and impurity in appearance, coarser white powder, low oil content on the surface of the microcapsule and embedding rate of more than 95%; accelerated experiments prove that the fish oil of the non-embedded hairtail offal is oxidized very rapidly, and the microencapsulation can greatly reduce the oxidation speed of the fish oil of the hairtail offal and prolong the storage time of grease. The product after embedding can be stored for 240 days at 20 ℃ through calculation, and the product has good stability.
TABLE 1 composition and content of fatty acids in fish oil refined from hairtail offal
Example 2
Study of the effect of lipid microcapsules on brown fat in mice.
The experimental method comprises the following steps:
the lipid microcapsules prepared in example 1 were used to feed mice.
After taking 30C 57B16 male mice with a body weight of 15-20 g at 5 weeks of age and feeding adaptively for 1 week, the mice were randomly divided into 3 groups according to body weight, namely a blank group (Control), a high fat group (HFD) and a microcapsule group (HFD+MC), 10 in each group. Wherein, the blank group is fed by normal feed, and the rest two groups are fed by high-fat feed. All mice are subjected to gastric lavage treatment, the gastric lavage content of a blank group and a high-fat group is physiological saline, the gastric lavage content of a microcapsule group is grease microcapsule solution, the administration volume is 10mL/kg.d, and the administration amount of the grease microcapsule after conversion is 0.6g/kg.d. The mice are fed in a constant temperature and constant humidity environment (25+/-2) DEG C, the circadian rhythm of 12h/12h (8:00-20:00 is turned on), the mice can eat and drink water freely, and the weight, the food intake and the water intake of the mice are monitored during the feeding period of the mice. The specific feeding protocol is shown in Table 2.
All mice were subjected to a cold exposure experiment at a fixed temperature of 8 ℃ for a fixed time of 8 hours per day for a period of 5 days on day 28. Each group of mice was placed in a metabolism cage after cold exposure for a metabolism cage experiment of 48 hours in length, and the carbon dioxide production and oxygen consumption of the mice during this period were measured, with a sampling period of 60s. Mice were subjected to glucose tolerance experiments after cold exposure. Serum fatty acid was measured before dissection of mice, 3mL of blood was taken through the tail vein, left standing for 10min, centrifuged at 3000 r/min for 10min at 4℃and serum was collected and measured using a Rili 7600-110 full-automatic biochemical analyzer, the measurement indexes included total Triglycerides (TG), total Cholesterol (TCHO), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C).
After the mice were continuously perfused for 32 days, all mice were fasted for 12 hours and subjected to CO 2 Dislocation is killed after anesthesia, brown adipose tissue and subcutaneous adipose tissue of the shoulder blade are separated, the tissues are placed in Phosphate Buffer (PBS) for cleaning for 3 times, and the tissues are transferred into a sterile 1.5mL centrifuge tube for freezing and preserving at the temperature of minus 80 ℃ for standby. Dissecting partial brown adipose tissue of a mouse, fixing the dissected brown adipose tissue in 4% paraformaldehyde, dehydrating and embedding the adipose tissue in paraffin, slicing, performing immunohistochemical staining detection on the brown adipose tissue, and observing Ucp1 expression of the brown adipose tissue under a light microscope; the target protein is marked by using a Prohibitin antibody (Abcam 75771) after dewaxing the brown adipose tissue sections, the result is observed by using a fluorescence microscope after the experiment is finished, the images are collected, and the average fluorescence intensity of the images is measured by using Image J software and normalized. And the brown adipose tissue of three groups of mice was analyzed by transcriptomic sequencing technique.
Table 2 experimental mouse feeding protocol
Experimental results:
1. has no obvious toxic side effect
In the experimental drug administration intervention process, the conditions of abnormal behavior, abnormal hair and the like of the mice are not seen, and the symptoms of poisoning and anaphylactic reaction are not seen. Mice survived normally, and no dead mice appeared during the experiment.
2. Weight loss
As shown in fig. 1, the high-fat mice had significantly higher body weight than the empty mice after 32 days of intervention. The results of the two-factor repeated analysis of variance show that the final weight of the mice subjected to the intervention of the oil microcapsule is significantly lower than that of the high-fat group (p < 0.05), which indicates that the oil microcapsule has good effect on reducing the weight.
3. Regulating blood sugar and blood lipid level
The effects of the lipid microcapsules on the blood sugar and blood lipid levels of mice are shown in fig. 2 and 3, respectively. The two-factor repeated analysis of variance results show that the blood sugar level of the mice in the high-fat group is extremely higher than that of the mice in the blank group and the microcapsule group (p < 0.0005) after four weeks of intervention, and no significant difference exists between the blank group and the microcapsule group; compared with a blank group, the TG content of the microcapsule group is obviously reduced (p is less than 0.05), which indicates that the grease microcapsule has good effect of regulating blood sugar and blood fat.
4. Promoting energy consumption
As shown in fig. 4, the peak carbon dioxide production and total carbon dioxide production of the microcapsule group mice after cold exposure were greater than those of the high-fat group mice. As shown in FIG. 5, the RQ value of the microcapsule group is significantly greater than that of the high-fat group (p < 0.05) in the 6 th to 18h period in the metabolic cage experiment. The above results demonstrate that the lipid microcapsules promote energy expenditure in mice.
5. Promoting differentiation of brown fat
As shown in FIG. 6, the results of immunohistochemical staining of brown fat in mice of different treatment groups revealed that the number of brown adipocytes in mice increased after the lipid microcapsule was dried. Immunofluorescence staining showed that the microcapsule group significantly increased positive staining of the mitochondrial marker proshibiin protein (p < 0.05) compared to the blank group and the high-fat group (fig. 7), indicating that brown fat has obvious signs of differentiation, and that the lipid microcapsule can promote differentiation of brown fat cells from a physiological aspect.
6. Activation of brown fat activation
Transcriptomic sequencing of brown adipose tissue was performed on three groups of mice, and the total number of original reads was found to be between 42.0 and 62.4 million for the three experimental groups. After screening to obtain clean data, quality evaluation of the clean data showed that the distribution of Q20 and Q30 in each sample was greater than 90% and that 85.73% -91.72% of the data from each experimental group was uniquely mapped to the genome. These results indicate that the sequence has good quality and reliable transcriptome analysis results. Under the precondition that P.adjust <0.05 and absolute value of log2FC > 1, carrying out differential gene analysis on the high-fat group and the microcapsule group to find 486 differential genes altogether; the difference genes were analyzed for the blank group and the microcapsule group to find 219 difference genes altogether. Analysis of volcanic patterns of the different genes in each group revealed that the log2FC value of the important gene Ucp1 associated with mitochondrial thermogenesis in grease microcapsule-mediated mice was 2.5, indicating that it was significantly up-regulated. In the results of GO enrichment analysis (fig. 8) for each group of mice we found a total of 23 pathways associated with lipid metabolism and brown fat activity, including cAMP metabolic process, fatty acid metabolism, G protein coupled chemotactic receptor activity, the top ten of which were in the ranking of gene upregulation fold associated with lipid metabolism: mup11, pla2g2e, ucp1, aspg, ccr10, nr1d1, acot12, gldc, gm49320, syt14. These results may indicate that the lipid microcapsules are able to promote the thermogenic effect of brown fats.
By performing a KEGG enrichment analysis of the differential genes for each group of mice (fig. 9), we found a total of 13 pathways relevant to this study, including PPAR signaling pathways. Significant upregulation of the genes Gk, scd3, ucp1, fabp5 was found on the PPAR signaling pathway in the microcapsule group, confirming the upregulation of PPAR signaling pathway by the lipid microcapsules.
The transcriptomic analysis results of the three groups of mice based on the differential genes show that the lipid microcapsule realizes the regulation and control effect on the activation and differentiation of brown adipose tissues mainly by up-regulating cAMP signal pathway and PPAR signal pathway on the transcriptional level.
The experimental results show that the grease microcapsule can promote brown fat activation by promoting differentiation of brown fat and expression of genes related to heat generation and enhancing heat generation efficiency of mitochondria, thereby reducing weight of mice and playing a role in treating obesity.
The foregoing description of the preferred embodiments of the invention has been presented only in terms of a specific embodiment, and it will be appreciated that variations and modifications may be made in the preferred embodiments of the invention without departing from the principles and scope of the invention.
Claims (9)
1. A method for preparing grease microcapsules with the function of activating brown fat, which is characterized by comprising the following steps:
(1) Preparation of an aqueous phase: adding maltodextrin into warm water for dissolution, adding octenyl succinic acid esterified starch according to a certain mass ratio, and stirring uniformly to prepare a water phase;
(2) Preparation of an oil phase: taking hairtail offal as a raw material, extracting by a microwave-assisted ethanol method to obtain hairtail offal crude fish oil, and refining by degumming, deacidifying, decoloring and winterizing methods to obtain hairtail offal refined fish oil; refined fish oil and glyceryl triacetate from hairtail scraps are taken as microcapsule core materials, and sucrose ester and glyceryl monostearate serving as emulsifying agents are added into the microcapsule core materials and heated for dissolution, so that an oil phase is obtained;
(3) Adding the oil phase into the water phase, adding water according to the total solid content of 40wt%, performing high-speed dispersion and homogenization treatment to obtain stable emulsion, and performing freeze drying to obtain the oil microcapsule powder.
2. The method for preparing the grease microcapsule with the function of activating brown fat according to claim 1, wherein the preparation process of the hairtail offal crude fish oil comprises the following steps: and (3) drying the hairtail offal overnight with a proper amount of anhydrous sodium sulfate, mixing the hairtail offal with absolute ethyl alcohol at a ratio of 1:6g/mL, carrying out microwave for 10min at a temperature of 75 ℃ in a microwave extraction instrument, filtering to remove hairtail offal residues, collecting filtrate, and carrying out rotary evaporation to obtain the hairtail offal crude fish oil.
3. The method for preparing the grease microcapsule with the brown fat activating function according to claim 2, wherein the mass ratio of hairtail offal to anhydrous sodium sulfate is 2:1.
4. The method for preparing the grease microcapsule with the brown fat activating function according to claim 1, wherein the mass ratio of octenyl succinic acid esterified starch to maltodextrin in the step (1) is 2:1.
5. The method for preparing the grease microcapsule with the brown fat activating function according to claim 1, wherein the mass ratio of the fish scrap refined fish oil to the glyceryl triacetate in the step (2) is 2:1, the mass ratio of sucrose ester to glyceryl monostearate is 9:1, and the amount of the emulsifier is 1% of the total mass of the fish scrap refined fish oil and the glyceryl triacetate.
6. The method for preparing the grease microcapsule with the brown fat activating function according to claim 1, wherein the mass ratio of the oil phase to the water phase in the step (3) is 1:2, the rotating speed of the high-speed dispersion homogenizer is 10000r/min, and the working time of the high-speed dispersion homogenizer is 1 min.
7. The method for preparing a lipid microcapsule with a brown fat activating function according to claim 1, wherein the specific condition of the freeze-drying in the step (3) is-50 ℃ and freezing for 24 hours under 50 Pa.
8. Grease microcapsule with brown fat activating function prepared by the preparation method according to any one of claims 1-7.
9. The application of the grease microcapsule with the brown fat activating function according to claim 8 is characterized in that the grease microcapsule is applied to the preparation of a weight-losing and lipid-lowering health product or a medicament for preventing/relieving cardiovascular and cerebrovascular diseases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310331009.XA CN116076755A (en) | 2023-03-31 | 2023-03-31 | Preparation method of grease microcapsule with brown fat activating function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310331009.XA CN116076755A (en) | 2023-03-31 | 2023-03-31 | Preparation method of grease microcapsule with brown fat activating function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116076755A true CN116076755A (en) | 2023-05-09 |
Family
ID=86201070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310331009.XA Pending CN116076755A (en) | 2023-03-31 | 2023-03-31 | Preparation method of grease microcapsule with brown fat activating function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116076755A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839047A (en) * | 2012-09-12 | 2012-12-26 | 连云港职业技术学院 | Method for extracting fish oil and producing taurine seafood condiments by using hairtail and leftovers of hairtail |
| CN107041554A (en) * | 2017-05-03 | 2017-08-15 | 赣州华汉生物科技有限公司 | Bata-carotene microcapsules rich in pilchard oil and preparation method and application |
| CN107660797A (en) * | 2017-10-11 | 2018-02-06 | 威海海日水产食品有限公司 | A kind of algin is the preparation method of the anti-aging soft capsule preparation of capsule material |
| CN107822142A (en) * | 2017-11-21 | 2018-03-23 | 荣成海锐芯生物科技有限公司 | A kind of method that cod liver oil capsule is prepared from cod internal organ |
| CN112704126A (en) * | 2020-12-18 | 2021-04-27 | 浙江远大生物科技有限公司 | Preparation method of oil microcapsule |
| KR102516220B1 (en) * | 2022-02-03 | 2023-03-30 | 주식회사 디와이 | Method for producing tongue sole fish paste and tongue sole paste produced by the same |
-
2023
- 2023-03-31 CN CN202310331009.XA patent/CN116076755A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839047A (en) * | 2012-09-12 | 2012-12-26 | 连云港职业技术学院 | Method for extracting fish oil and producing taurine seafood condiments by using hairtail and leftovers of hairtail |
| CN107041554A (en) * | 2017-05-03 | 2017-08-15 | 赣州华汉生物科技有限公司 | Bata-carotene microcapsules rich in pilchard oil and preparation method and application |
| CN107660797A (en) * | 2017-10-11 | 2018-02-06 | 威海海日水产食品有限公司 | A kind of algin is the preparation method of the anti-aging soft capsule preparation of capsule material |
| CN107822142A (en) * | 2017-11-21 | 2018-03-23 | 荣成海锐芯生物科技有限公司 | A kind of method that cod liver oil capsule is prepared from cod internal organ |
| CN112704126A (en) * | 2020-12-18 | 2021-04-27 | 浙江远大生物科技有限公司 | Preparation method of oil microcapsule |
| KR102516220B1 (en) * | 2022-02-03 | 2023-03-30 | 주식회사 디와이 | Method for producing tongue sole fish paste and tongue sole paste produced by the same |
Non-Patent Citations (1)
| Title |
|---|
| 黄万有;李德涛;屈小娟;刘书成;郝记明;张静;: "人工神经网络优化军曹鱼内脏鱼油酶法提取工艺参数", 食品工业科技, no. 07, 1 April 2013 (2013-04-01), pages 173 - 177 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yin et al. | Dietary supplementation of bile acid attenuate adverse effects of high-fat diet on growth performance, antioxidant ability, lipid accumulation and intestinal health in juvenile largemouth bass (Micropterus salmoides) | |
| CN109907126B (en) | Grease composition of medium-long carbon chain triglyceride and preparation method thereof | |
| Yuan et al. | Hypoglycemic and anti-inflammatory effects of seabuckthorn seed protein in diabetic ICR mice | |
| Li et al. | Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet | |
| Fotschki et al. | Dietary supplementation with raspberry seed oil modulates liver functions, inflammatory state, and lipid metabolism in rats | |
| Omar et al. | GC-MS analysis of chemical constituents in ethanolic bee pollen extracts from three species of Malaysian stingless bee | |
| Wang et al. | Microencapsulated duck oil diacylglycerol: Preparation and application as anti-obesity agent | |
| Peng et al. | Protective effects of Sapindus mukorossi Gaertn against fatty liver disease induced by high fat diet in rats | |
| Lee et al. | Anti-inflammatory effects of Agar free-Gelidium amansii (GA) extracts in high-fat diet-induced obese mice | |
| WO2015021631A1 (en) | Astragalus seed raw oil, refined oil, preparation method therefore and application thereof | |
| Qiu et al. | Biosafety evaluation of Nannochloropsis oculata and Schizochytrium sp. oils as novel human milk fat substitutes | |
| CN116076755A (en) | Preparation method of grease microcapsule with brown fat activating function | |
| CN111789078B (en) | Method for establishing rat non-obese non-alcoholic fatty liver disease model | |
| CN105341184A (en) | Functional fat composition having effects of preventing cardiovascular and cerebrovascular diseases and diabetes risk factors | |
| JP7520175B2 (en) | Method for increasing blood decanoic acid concentration, agent for increasing blood decanoic acid concentration, pharmaceutical composition, and food composition | |
| Yang et al. | Chlorella unsaturated fatty acids suppress high‐fat diet‐induced obesity in C57/BL6J mice | |
| Guo et al. | Elaeagnus mollis oil attenuates non-alcoholic fatty disease in high-fat diet induced obese mice via modifying the expression of lipid metabolismrelated genes | |
| Hu et al. | Physicochemical properties and nutritional ingredients of kernel oil of Carya cathayensis Sarg | |
| Shetty et al. | Virgin coconut oil (VCO) ameliorates high fat diet (HFD)-induced obesity, dyslipidemia and bestows cardiovascular protection in rats | |
| Smith et al. | Impact of diet containing grape pomace on growth performance and blood lipid profile of young rats | |
| CN1230183C (en) | Use of dictyoptera grease in healthcare food and medicine , its preparation method | |
| CN101658576B (en) | Purpose of pummelo peel general flavone in preparing medicament for treating alcoholic liver injury | |
| ZHANG et al. | Diacylglycerol-enriched oil from hydrolysis of soybean oil with rhizopus oryzae lipase against high-fat diet-induced obesity in mice | |
| RU2197977C2 (en) | Pumpkin seed oil eliciting anti-inflammatory, antiulcerous and hepatoprotective effect and method of its preparing | |
| SILALAHI et al. | The effect of Acute Consumption of Hydrolyzed Oils on Swimming Capacity Endurance of Mice (Mus Musculus) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |