CN116064296A - Mesotrione degrading bacterium Klebsiella pasteurii CM-1 and application thereof - Google Patents
Mesotrione degrading bacterium Klebsiella pasteurii CM-1 and application thereof Download PDFInfo
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- 230000000593 degrading effect Effects 0.000 title claims abstract description 59
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- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
The invention relates to mesotrione degrading bacteria Klebsiella pasteurii CM-1 and application thereof. The CM-1 strain is preserved in China general microbiological culture Collection center (CGMCC) at 25 th month of 2022, with a preservation number of CGMCC No.25396 and a preservation address of Beijing Kogyang North Chen Xiyu No. 1 and 3. The CM-1 strain disclosed by the invention has the advantages of strong biodegradability of mesotrione, high degradation speed and high degradation efficiency, can stably degrade the mesotrione, is used for bioremediation of environments such as water body, soil and the like polluted by the mesotrione, and can be used for preparing a mesotrione degrading preparation or a mesotrione pollution restoring preparation in the environment. The strain provided by the invention enriches a strain resource library of mesotrione degrading bacteria, and provides a new choice for mesotrione degradation and bioremediation of mesotrione pollution.
Description
Technical Field
The invention belongs to the technical field of biodegradation of pesticides and biological treatment of environmental pollutants, and particularly relates to mesotrione degrading bacteria Klebsiella pasteurii CM-1 and application thereof.
Background
Mesotrione (mesotrione) is a selective pre-emergence and post-emergence trione herbicide commonly used for controlling annual broadleaf weeds and part of grassy weeds in corn, rice, sugarcane and other crop fields. By 2018, sales of mesotrione in China are the second to be worldwide, and the use amount of mesotrione in China is large. The mesotrione has higher initial activity and residual activity and stronger water solubility and leaching property, so that the mesotrione is very easy to flow into surface water or infiltrate into underground water in a runoff or leaching mode, thereby causing serious pollution to an aquatic system and further causing poisoning or death of non-target organisms. The mesotrione has stable chemical property, the photolysis half-life in aqueous solution is as long as 84d, the degradation half-life in soil is 3-32 d, and various aftercrop can be greatly reduced in yield and even be in failure due to residual mesotrione.
In recent years, the use amount and the application frequency of mesotrione are continuously increased, excessive mesotrione is accumulated in the environment, the corn sowing area in China is continuously increased along with the rapid development of the planting industry, the use amount of mesotrione is always increased again as one of main herbicides in corn fields, and the residual pollution in the environment is also increasingly serious. The existence of excessive mesotrione residue in the environment presents serious potential threat to human health and ecological balance, and the development of a method capable of efficiently and safely degrading mesotrione residue is particularly important.
Compared with the high cost of repairing by a physical method and a chemical method and the disadvantage of easily causing secondary pollution, the method for degrading the pesticide residue by utilizing microorganisms has the advantages of high efficiency, low cost, difficulty in causing secondary pollution and the like, and has been paid more attention and more attention.
At present, researches on the degradation of mesotrione by microorganisms have also been reported, wherein degrading bacteria which have the capability of degrading mesotrione are disclosed at home and abroad, wherein the degrading bacteria comprise Bacillus sp.3B6 (doi: 10.1111/j.1472-765X.2006.01923. X), pantoea ananatis (doi: 10.1016/j.chemosphere.2011.12.041) and Bradyrhizobium sp.SR1 (doi: 10.1007/s 11356-015-4544-1), and Chinese patent No. CN 103667240A also discloses a degrading bacterium Bcaillus pumilus HZ-2 for degrading pesticides, wherein the pesticide degradable by the strain comprises mesotrione. Although the prior art discloses partial mesotrione degrading bacteria, the mesotrione degrading treatment in the environment is still limited, and the change of the environmental conditions often affects the degrading capability of microorganisms on pesticides, so that the existing mesotrione degrading bacteria resources are far from sufficient to meet the requirements of microorganisms on mesotrione degradation. The strain is used as a pure culture body, the individual specificity is strong, and the strain is greatly influenced by the environment. Therefore, it is necessary to continuously dig and search new microbial resources for degrading residual pesticides with better control effect. Therefore, the continuous screening of novel microbial resources with the capability of efficiently degrading mesotrione is important.
Disclosure of Invention
The invention aims to overcome the defects of the existing mesotrione microbial degradation means and provide mesotrione degradation bacteria Klebsiella pasteurii CM-1 and application thereof. The CM-1 strain is separated from farmland soil for long-term application of mesotrione in Fu-Suly county of Guangxi Zhuang nationality, has high-efficiency and rapid degradation capability on mesotrione, can be used for degradation of mesotrione in the environment and repair of mesotrione pollution in the environment, can be used for preparing mesotrione degradation microbial inoculum, enriches mesotrione degradation microbial libraries, and provides a new choice for degradation of residual mesotrione in the environment.
The invention aims to provide mesotrione degrading bacteria Klebsiella pasteurii CM-1.
The invention also aims to provide the application of the mesotrione degrading bacterium Klebsiella pasteurii CM-1 in mesotrione degradation and/or in the restoration of mesotrione pollution in the environment.
The invention also aims to provide the application of the mesotrione degrading bacterium Klebsiella pasteurii CM-1 in preparation of mesotrione degrading preparations and/or preparation of mesotrione pollution repairing preparations.
The invention also aims to provide a mesotrione degradation preparation.
The invention also aims to provide a preparation for repairing mesotrione pollution in the environment.
The invention also aims to provide the application of the preparation in mesotrione degradation and/or mesotrione pollution restoration in the environment.
The invention also aims to provide a method for degrading mesotrione and/or repairing mesotrione pollution in the environment.
The above object of the present invention is achieved by the following technical means:
the invention provides mesotrione degrading bacteria Klebsiella pasteurii CM-1, which are preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 25396 and the preservation address of North Chen Xiyu No. 1 and 3 in the Chaoyang area of Beijing city at 2022, and 7 and 25 days.
The Klebsiella pasteurii CM-1 strain is derived from farmland soil of Fuseidone applied to Fuseium county of Chongleft, china in Guangxi Zhuang nationality, and is obtained through artificial enrichment culture, separation and purification. The CM-1 strain has high-efficiency and rapid degradation capability on mesotrione, the degradation rate of mesotrione of 100mg/L after 16h culture is more than 99%, and the CM-1 strain can be used as an excellent biodegradation bacterium for mesotrione degradation or the bioremediation of mesotrione pollution in the environment.
Therefore, the following applications are within the scope of the present invention.
The mesotrione degrading bacterium Klebsiella pasteurii CM-1 is applied to mesotrione degradation and/or mesotrione pollution restoration in the environment.
The mesotrione degrading bacterium Klebsiella pasteurii CM-1 is applied to preparation of mesotrione degrading preparations and/or preparation of mesotrione pollution repairing preparations.
A formulation for degrading mesotrione and/or repairing mesotrione pollution in an environment, said formulation comprising said mesotrione degrading bacterium Klebsiella pasteurii CM-1.
Preferably, the environment is a body of water and/or soil.
Preferably, the preparation contains thalli and/or bacterial liquid of the mesotrione degrading bacteria Klebsiella pasteurii CM-1.
Preferably, the preparation contains thalli and bacterial liquid of the mesotrione degrading bacteria Klebsiella pasteurii CM-1.
As a specific example, a preparation containing mesotrione degrading bacteria Klebsiella pasteurii CM-1 is obtained by inoculating mesotrione degrading bacteria Klebsiella pasteurii CM-1 strain into a liquid culture medium, culturing at 30deg.C for 24 hr/min, and collecting the culture solution.
The preparation is applied to degradation of mesotrione and/or repair of mesotrione pollution in the environment.
A method for degrading mesotrione and/or repairing mesotrione pollution in the environment, using said mesotrione degrading bacterium Klebsiella pasteurii CM-1 or said formulation. The preparation contains the mesotrione degrading bacterium Klebsiella pasteurii CM-1, thalli and/or bacterial liquid containing the mesotrione degrading bacterium Klebsiella pasteurii CM-1, and thalli and bacterial liquid containing the mesotrione degrading bacterium Klebsiella pasteurii CM-1.
Preferably, the formulation is utilized; the preparation contains the mesotrione degrading bacterium Klebsiella pasteurii CM-1, thalli and/or bacterial liquid containing the mesotrione degrading bacterium Klebsiella pasteurii CM-1, and thalli and bacterial liquid containing the mesotrione degrading bacterium Klebsiella pasteurii CM-1.
Preferably, the formulation is utilized; the preparation is a preparation containing thalli and/or bacterial liquid of the mesotrione degrading bacteria Klebsiella pasteurii CM-1.
Preferably, the formulation is utilized; the preparation is a preparation containing thalli and bacterial liquid of the mesotrione degrading bacteria Klebsiella pasteurii CM-1.
Compared with the prior art, the invention has the following beneficial effects:
the Klebsiella pasteurii CM-1 p-mesotrione provided by the invention has the advantages of strong biodegradability, high degradation speed and high degradation efficiency, can stably degrade mesotrione, can be used for bioremediation of environments such as water body, soil and the like polluted by mesotrione, and can be used for preparing a mesotrione degradation preparation or a mesotrione pollution restoration preparation in the environment. The invention provides theoretical support for the application of the mesotrione high-efficiency degradation bacteria, enriches the strain resource library of the mesotrione degradation bacteria, and provides an effective method and a new choice for the degradation of mesotrione and the bioremediation of mesotrione pollution.
Drawings
FIG. 1 shows colony morphology of the CM-1 strain in example 1 of the present invention.
FIG. 2 is a phylogenetic tree of the CM-1 strain in example 1 of the present invention.
FIG. 3 is a high performance liquid chromatogram of mesotrione in example 2 of the present invention, wherein a represents mesotrione concentration of 0.05mg/L and b represents mesotrione concentration of 10.00mg/L.
FIG. 4 is a standard curve of mesotrione content (x) versus peak area (y) in example 2 of the present invention.
Detailed Description
The present invention is further illustrated below with reference to specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Reagents and materials used in the following examples are commercially available unless otherwise specified.
Inorganic salt liquid medium: KH (KH) 2 PO 4 3.00g，Na 2 HPO 4 6.00g，MgSO 4 0.01g of Glucose (Glucose) 8.00g,NaCl 5.00g,NH 4 Cl 2.00g, deionized water to 1000mL, pH 7.0, and autoclaving at 121℃for 30min.
Inorganic salt solid medium: agar 20.00g, KH 2 PO 4 3.00g，Na 2 HPO 4 6.00g，MgSO 4 0.01g of Glucose (Glucose) 8.00g,NaCl 5.00g,NH 4 Cl 2.00g, deionized water to 1000mL, pH 7.0, 121℃autoclave for 30min.
LB solid medium: agar 20.00g, tryptone 10.00g, yeast extract 5.00g, naCl10.00g, deionized water to 1000mL, pH=7.0, and autoclaved at 121℃for 30min.
LB liquid medium: tryptone 10.00g, yeast extract 5.00g,NaCl10.00g, deionized water to 1000ml, ph=7.0, autoclaved at 121 ℃ for 30min.
The inoculum sizes described in the examples below were all inoculated in the corresponding volume ratios (V: V).
Example 1 isolation and identification of mesotrione degrading bacteria
1. Separation of mesotrione degrading bacteria
5.00g of farmland soil for long-term application of mesotrione in Fu-Sui county, guangxi Zhuang nationality, left, is taken and put into 100mL of inorganic salt liquid culture medium containing 100mg/L of mesotrione, enrichment culture is carried out at 30 ℃ and 150r/min, and each 7d of farmland soil is transferred into fresh inorganic salt liquid culture medium containing 100mg/L of mesotrione according to the inoculation amount of 10% (10 mL) for 4 times continuously.
Taking 0.2mL of bacterial liquid obtained in the last transfer, adding into 1.8mL of sterile water to prepare 10 -1 0.2mL of the prepared 10 is taken again -1 Is added into 1.8mL of sterile water to prepare 10 -2 And so on, the bacterial liquid is gradually diluted to 10 -5 Multiple times. Suction 10 -5 0.1mL of the dilution liquid of (2) is coated on a solid culture medium plate containing 100mg/L of mesotrione inorganic salt, after the inversion culture is carried out for 2 days at the constant temperature of 30 ℃, single colonies with degradation rings around are picked up and streaked on an LB solid culture medium plate, and the purification is repeated for at least 3 times until only one single colony exists on the plate.
Picking single colony to enrich and culture in LB liquid culture mediumAfter 12h, centrifuging at 8000r/min for 10min, discarding supernatant, washing with PBS buffer solution for 2 times, adding PBS buffer solution, and adjusting OD 600 The bacterial suspension is obtained with the value of 1.8, the bacterial suspension is inoculated into 50mL of inorganic salt liquid culture medium containing 100mg/L mesotrione with the inoculum size of 5 percent (2.5 mL) to be used as a treatment group, PBS buffer solution is inoculated into 50mL of inorganic salt liquid culture medium containing 100mg/L mesotrione with the inoculum size of 5 percent (2.5 mL) to be used as a control group, mesotrione in the culture medium is extracted after 3d culture at 30 ℃ for verification of degradation effect, and a bacterial strain can be efficiently degraded by the bacterial strain, and the bacterial strain is found to be CM-1 through measurement.
Morphological characterization of CM-1 Strain
The CM-1 strain was inoculated onto LB solid medium plates, cultured upside down at 30℃for 2d, and the colony morphology was observed.
The colony morphology of the CM-1 strain is shown in FIG. 1, and FIG. 1 shows that the colony of the CM-1 strain is white, round, convex at the edge and sticky in texture, and the produced soluble pigment dyes the LB culture medium plate into dark yellow, and gram staining is negative.
Molecular biological identification of CM-1 Strain
The genomic DNA of CM-1 strain was extracted, and its 16s rRNA was PCR amplified using universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3') using the extracted genomic DNA as a template, and the PCR product was sent to Hua Dacron science and technology service Co., ltd (Shenzhen) for sequencing.
The nucleotide sequence of 16s rRNA is:
TCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAGAGAGCTTGCTCTCGGGTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGGTTAAGGTTAATAACCTCATCCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTGGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGTCGACTTGGAGGTTGTTCCCTTGAGGAGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTACTCTTGACATCCAGAGAACTTAGCAGAGATGCTTTGGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACACGTGCTACAATGGCATATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTATGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGTAGCTAACCTTCGGGAGGCGCTTACCACTTTGTGATCATGACTGGGGTGA(SEQ ID NO：1)。
sequencing results BLAST comparison was performed in NCBI, and phylogenetic tree was constructed by using MEGA 7.0 software according to the comparison results, and as shown in FIG. 2, the CM-1 strain was found to have the highest homology with Klebsiella pasteurii by combining the BLAST comparison results with the phylogenetic tree, and the closest evolutionary distance was identified as Klebsiella pasteurii, and was named Klebsiella pasteurii CM-1.
Klebsiella pasteurii CM-1 is preserved in China general microbiological culture Collection center (CGMCC) No.25396 at 7.25 of 2022, and has a preservation address of North Chen Xili No. 1 and 3 of the Korean region of Beijing city.
Example 2 Klebsiella pasteurii CM-1 determination of degradation Properties of Parafocalycyr
1. Method of
(1) Preparation of bacterial suspension
Inoculating Klebsiella pasteurii CM-1 strain obtained in example 1 after purification into LB liquid medium, culturing for 12 hr, centrifuging at 8000r/min for 10min, discarding supernatant, washing with PBS buffer solution for 2 times, adding PBS buffer solution, and adjusting OD 600 The value is 1.8, and the bacterial suspension is obtained.
(2) Degradation Performance measurement
The bacterial suspension prepared in the example (1) was inoculated into 50mL of an inorganic salt liquid medium containing 100mg/L mesotrione as a treatment group, PBS buffer was inoculated into 50mL of an inorganic salt liquid medium containing 100mg/L mesotrione as a control group, 3 replicates were set for each of the treatment group and the control group, and the culture was carried out at 35℃under 150r/min conditions, and samples were taken at 0h, 6h, 12h, 16h and 20h, respectively, and the mesotrione content in the culture solution was measured by High Performance Liquid Chromatography (HPLC).
(3) HPLC chromatographic conditions
The instrument was a Waters-E2695 HPLC, the chromatographic conditions were Agilent ZORBAX Eclipse XDB-C18 chromatographic column (50 mm. Times.4.6 mm,5 μm), the column temperature was 30℃and the mobile phase ratio was A (acetonitrile): b (0.05% phosphoric acid-water solution) =40: 60 (V: V), flow rate 1mL/min, sample volume 20. Mu.L, detection wavelength 230nm.
The degradation of the mesotrione Klebsiella pasteurii CM-1 is expressed in terms of degradation rate.
Degradation rate (R) calculation formula: r/% = [1- (C) t /C 0 )]X 100; wherein C is t To treat group mesotrione concentration, C 0 The unit is mg/L for the control mesotrione concentration.
(4) Standard Curve establishment
10000mg/L of mesotrione standard working solution is prepared by acetonitrile, diluted to 0.05, 0.10, 1.00, 5.00 and 10.00mg/L respectively, and measured by HPLC after passing through an organic film of 0.22 mu m, and a standard curve is established according to the content and peak area of mesotrione.
(5) Sample processing method
Taking culture solutions with different culture times, centrifuging at 8000r/min for 10min, taking 1mL of supernatant, adding into a centrifuge tube containing 9mL of deionized water, diluting for 10 times, mechanically oscillating for 5min to uniformly mix, taking 2mL of diluent, passing through a 0.22 μm water film, and measuring by using HPLC.
(6) Measurement of recovery by addition
10000mg/L mesotrione standard working solution is prepared by acetonitrile, 10mL of inorganic salt liquid is taken for culture and is based on a 50mL centrifuge tube, a certain amount of mesotrione standard working solution is respectively added into the centrifuge tube, the concentration of the mesotrione standard working solution is respectively 1.00, 5.00 and 10.00mg/L, 3 times of repetition are respectively arranged, the mechanical oscillation is carried out for 10 minutes, and after standing for 10 minutes, 1mL of supernatant is taken, and after passing through a 0.22 mu m water film, the HPLC is used for measurement, and the recovery rate is calculated.
The recovery rate calculation formula: recovery = C 1 /C 0 X 100%; wherein C is 1 Adding a measurement of mesotrione to the blank sample; c (C) 0 The measurement value of mesotrione with the same quantity is added to the blank sample extracting solution, and the unit is mg/L.
2. Results
(1) Chromatograms and standard curves of mesotrione
The high performance liquid chromatogram of mesotrione is shown in FIG. 3, wherein a represents mesotrione concentration of 0.05mg/L and b represents mesotrione concentration of 10.00mg/L. As can be seen from FIG. 3, the mesotrione can be detected at both 0.05mg/L and 10.00mg/L by the detection method of this example, the peak time is 8.3min, the peak shape is sharp, the separation is complete and no interference peak appears.
As can be seen from FIG. 4, the standard curve of mesotrione content (x) and peak area (y) is shown in FIG. 4, and the two are in good linear relationship, the standard curve equation is y= 50412x-3397.2, R 2 ＝0.9991。
(2) Recovery rate of addition
The results of the addition recovery test of mesotrione in an inorganic salt liquid medium (n=3) are shown in table 1.
TABLE 1
From Table 1, the recovery rate of mesotrione in the inorganic salt liquid culture medium is 83.35% -95.11%, which shows that the mesotrione extraction and recovery method has good accuracy and meets the detection requirement.
(3) Degradation Performance measurement results
The results of measuring the degradation properties of Klebsiella pasteurii CM-1 p-mesotrione are shown in Table 2.
TABLE 2
As is clear from Table 2, after culturing in an inorganic salt liquid medium containing 100mg/L mesotrione for 6 hours, 12 hours, 16 hours and 20 hours, the degradation rates of Klebsiella pasteurii CM-1 on 100mg/L mesotrione respectively reached 16.71%, 88.52%, 99.49% and 100%, and the mesotrione in the control group did not naturally degrade within 20 hours. After 16h of culture, the degradation rate of Klebsiella pasteurii CM-1 to 100mg/L mesotrione is over 99 percent, which shows that Klebsiella pasteurii CM-1 has the capability of rapidly and efficiently degrading mesotrione.
Example 3 preparation of Klebsiella pasteurii CM-1 microbial inoculum
Inoculating the purified Klebsiella pasteurii CM-1 strain obtained in the example 1 into an LB liquid culture medium, culturing at 30 ℃ for 24 hours at 150r/min, and collecting a culture solution to obtain the Klebsiella pasteurii CM-1 microbial inoculum. The mesotrione in the sample can be degraded by mixing the microbial inoculum with the sample containing mesotrione.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (10)
1. The mesotrione degrading bacterium Klebsiella pasteurii CM-1 is characterized by being preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 25396 and the preservation address of North Chen Xiyu No. 1 and No. 3 in the Korean region of Beijing city at the day of 7 and 25 of 2022.
2. The use of mesotrione degrading bacteria Klebsiella pasteurii CM-1 as claimed in claim 1 for degrading mesotrione and/or for repairing mesotrione pollution in an environment.
3. The use of mesotrione degrading bacterium Klebsiella pasteurii CM-1 according to claim 1 in the preparation of mesotrione degrading formulations and/or in the preparation of formulations for the remediation of mesotrione pollution in an environment.
4. A formulation for degrading mesotrione and/or repairing mesotrione pollution in an environment, said formulation comprising mesotrione degrading bacteria Klebsiella pasteurii CM-1 according to claim 1.
5. The preparation according to claim 4, which comprises the mesotrione degrading bacterium Klebsiella pasteurii CM-1 according to claim 1.
6. The preparation according to claim 5, which comprises the mesotrione degrading bacterium Klebsiella pasteurii CM-1 according to claim 1, and a bacterial liquid.
7. Use of a formulation according to any one of claims 4 to 6 for degrading mesotrione and/or for repairing mesotrione pollution in the environment.
8. A method for degrading mesotrione and/or repairing mesotrione pollution in an environment, characterized in that mesotrione degrading bacteria Klebsiella pasteurii CM-1 according to claim 1 or a formulation according to any one of claims 4-6 is used.
9. The method according to claim 8, characterized in that the formulation according to any one of claims 4 to 6 is used.
10. The method according to claim 9, characterized in that the formulation according to claim 5 or 6 is used.
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| CN103667240A (en) * | 2013-11-29 | 2014-03-26 | 华南农业大学 | Pesticide degrading bacterium preparation as well as preparation method and application thereof |
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| CN103667240A (en) * | 2013-11-29 | 2014-03-26 | 华南农业大学 | Pesticide degrading bacterium preparation as well as preparation method and application thereof |
| WO2020254711A1 (en) * | 2019-06-21 | 2020-12-24 | Servicio Andaluz De Salud | Kit and method for rapid detection of bacteria resistant to combinations of β-lactam antibiotic with a β-lactamase inhibitor, and for rapid detection of extended-spectrum resistance to β-lactams in clinical isolates |
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