CN114231422A - Fusarium solani for degrading fomesafen and application thereof - Google Patents
Fusarium solani for degrading fomesafen and application thereof Download PDFInfo
- Publication number
- CN114231422A CN114231422A CN202111490799.3A CN202111490799A CN114231422A CN 114231422 A CN114231422 A CN 114231422A CN 202111490799 A CN202111490799 A CN 202111490799A CN 114231422 A CN114231422 A CN 114231422A
- Authority
- CN
- China
- Prior art keywords
- fomesafen
- fusarium solani
- degrading
- strain
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- BGZZWXTVIYUUEY-UHFFFAOYSA-N fomesafen Chemical compound C1=C([N+]([O-])=O)C(C(=O)NS(=O)(=O)C)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 BGZZWXTVIYUUEY-UHFFFAOYSA-N 0.000 title claims abstract description 68
- 241000427940 Fusarium solani Species 0.000 title claims abstract description 60
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
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- C02F2101/38—Organic compounds containing nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09K2101/00—Agricultural use
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Abstract
The invention discloses fusarium solani for degrading fomesafen, and the name of the strain is as follows: F-F1, classified and named as: fusarium solani with the preservation number of CGMCC No.23288 and the preservation date of the Fusarium solani is as follows: 10/19/2021, depository: china general microbiological culture Collection center, West Lu No.1 Hospital No. 3, Beijing, Chaoyang, the area of the republic of China. The rDNA-ITS sequence of the strain F-F1 consists of 573 basic groups, the screened Fusarium solani is used as a single strain to co-metabolize herbicide Fusarium solani with a proper amount of nutrient substances, the change of the residual concentration of fomesafen in the continuous 7-day culture process is measured, and the result shows that the Fusarium solani F-F1 and the proper amount of nutrient substances can well degrade the fomesafen.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to fusarium solani for degrading fomesafen and application thereof.
Background
At present, the farmland soil quality condition in China is great, and the unreasonable use of chemical fertilizers and pesticides is one of the main reasons. Diphenylether herbicides (DEHs) are widely used worldwide due to their advantages of high efficiency, low toxicity, high selectivity, etc. Most of the currently used fluorine-containing varieties with high activity mainly comprise fomesafen, oxyfluorfen, acifluorfen, lactofen and the like. The fomesafen is applied to 80% -90% of soybean fields in China, and in order to promote agricultural structure optimization, the national planting industry structure is adjusted in 2020, and the soybean area is increased to 1.4 hundred million acres. With the succession of soybean farmland weed groups, the number of weeds insensitive to pesticides is increased, and the usage amount and application area of fomesafen are increased in recent years. The residual effect of the fomesafen accumulated in the soil is long, so that the residual quantity in the field is high due to continuous application for many years, the yield of sensitive crops such as Chinese cabbage, beet, corn, wheat and the like is reduced, even the crops are completely harvested, the adjustment of agricultural planting structures in Chinese food main production areas and the safety of agricultural production are seriously threatened, and the fomesafen also becomes an important factor of agricultural non-point source pollution in China. Therefore, how to clean and efficiently remove the residual toxicity of fomesafen in soil becomes a problem to be solved urgently at present. DEHs may also be digested by photodegradation, leaching, etc., but microbial degradation is the primary mode of degradation because many microorganisms have enzymes in their bodies that readily convert nitro groups. The method has important significance for exploring a new environment-friendly pollution remediation technology and realizing green sustainable development of agriculture by utilizing the main biological group of the soil to synergistically remediate the DEHs pollution in the farmland soil.
Currently, the study considers that fusarium solani is a soil inhabitant and can cause systemic or local infection of plants to cause plant wilting and rot. But simultaneously, researchers also find that the fusarium graminearum has an inhibiting effect on various pathogenic bacteria. The functions of Fusarium solani for producing medicine monomer components of rhein and emodin and degrading agricultural antibiotics of oxytetracycline, norfloxacin and sulfadimidine are also reported in research.
Through searching, no patent publication related to the present patent application has been found.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides fusarium solani for degrading fomesafen and application thereof.
The technical scheme adopted by the invention for solving the technical problem is as follows:
fusarium solani for degrading fomesafen, wherein the name of the strain is as follows: F-F1, classified and named as: fusarium solani with the preservation number of CGMCC No.23288 and the preservation date of the Fusarium solani is as follows: 10/19/2021, depository: china general microbiological culture Collection center, West Lu No.1 Hospital No. 3, Beijing, Chaoyang, the area of the republic of China.
Further, the rDNA-ITS sequence of the strain F-F1 is SEQ ID NO. 1.
Furthermore, the fusarium solani F-F1 can degrade the herbicide fomesafen under the co-metabolism of the additional nutrient substances, and the degradation rate of the fomesafen can reach 96.2%.
Further, the nutrient substances are basal salt culture medium + 2% (mass fraction) glucose;
wherein the basic salt culture medium is: 1.0g/L of ammonium sulfate, 1.5g/L of dipotassium hydrogen phosphate, 0.5g/L of potassium dihydrogen phosphate, 1.0g/L of sodium chloride, 0.4g/L of magnesium sulfate heptahydrate, distilled water for constant volume and hydrochloric acid for adjusting the pH value to be neutral; autoclaved at 121 ℃ for 20 minutes before use.
Comprising the fomesafen degrading agent of fusarium solani degrading fomesafen as described above.
Further, the reagent further comprises an external carbon source and/or nitrogen source.
Further, the reagent also comprises an external carbon source of glucose.
The fusarium solani is applied to the aspect of degrading fomesafen.
The fusarium solani is applied to the aspect of serving as a soil remediation agent.
The fusarium solani is applied to the aspect of serving as a water body repairing agent.
The beneficial effects obtained by the invention are as follows:
1. the rDNA-ITS sequence of the strain F-F1 consists of 573 basic groups, the screened Fusarium solani is used as a single strain to co-metabolize herbicide Fusarium solani with a proper amount of nutrient substances, the change of the residual concentration of fomesafen in the continuous 7-day culture process is measured, and the result shows that the Fusarium solani F-F1 and the proper amount of nutrient substances can well degrade the fomesafen.
2. The invention carries out the degradation and repair research of common herbicide fomesafen in the farmland with the combined metabolism of fusarium solani and nutrient glucose in the inorganic salt culture medium, and determines the change of the fomesafen in the culture medium in the 168h (7d) culture process. The fusarium can be used as a degrading strain to degrade fomesafen and can be used as a candidate strain for pollution remediation of the fomesafen serving as a farmland soil herbicide.
3. The invention is proved by tests that: the culture medium of the strain F-F1 is as follows: 2% glucoseIn the presence of 50mg L of the total metabolism-1When the fomesafen is cultured for 7 days, the degradation rate of the fomesafen in F-F1+ 2% Glucose (GFM) treatment can respectively reach 96.2%, and the degradation rate of the fomesafen in blank (CK) treatment is less than 4.4%. Whereas the degradation rate of fomesafen in the treatment (FM) with only the addition of fungi was only 19.5%.
4. The invention analyzes and tests the degradation effect of pure bacteria of the strain F-F1 on the herbicide fomesafen under the co-metabolism action of the additional nutrient substances, and the strain has the prospect of being used as a candidate strain for degrading and repairing the fomesafen serving as an agricultural herbicide.
Drawings
FIG. 1 is a diagram showing the colony morphology and microscopic features of Fusarium solani on potato dextrose agar medium of the present invention;
FIG. 2 is a diagram showing the growth of Fusarium solani in pure culture with the addition of carbon/nitrogen sources;
FIG. 3 is a graph showing the degradation profile of fomesafen in a blank basal salt medium and nutrient addition control according to the present invention;
FIG. 4 is a graph showing the degradation rate of fomesafen in the blank basal salt medium and nutrient co-metabolism of Fusarium solani of the present invention.
Detailed Description
The present invention will be further described in detail with reference to examples for better understanding, but the scope of the present invention is not limited to the examples.
The raw materials used in the invention are all conventional commercial products if not specified, the method used in the invention is all conventional in the field if not specified, and the mass of each substance used in the invention is all conventional use mass.
Fusarium solani for degrading fomesafen, wherein the name of the strain is as follows: F-F1, classified and named as: fusarium solani with the preservation number of CGMCC No.23288 and the preservation date of the Fusarium solani is as follows: 10/19/2021, depository: china general microbiological culture Collection center, West Lu No.1 Hospital No. 3, Beijing, Chaoyang, the area of the republic of China.
Preferably, the rDNA-ITS sequence of the strain F-F1 is SEQ ID NO. 1.
Preferably, the fusarium solani F-F1 can degrade the herbicide fomesafen under the co-metabolism of the additional nutrient substances, and the degradation rate of the fomesafen can reach 96.2%.
Preferably, the nutrient is a basic salt culture medium plus 2% (mass fraction) of glucose;
wherein the basic salt culture medium is: 1.0g/L of ammonium sulfate, 1.5g/L of dipotassium hydrogen phosphate, 0.5g/L of potassium dihydrogen phosphate, 1.0g/L of sodium chloride, 0.4g/L of magnesium sulfate heptahydrate, distilled water for constant volume and hydrochloric acid for adjusting the pH value to be neutral; autoclaved at 121 ℃ for 20 minutes before use.
Comprising the fomesafen degrading agent of fusarium solani degrading fomesafen as described above.
Preferably, the reagent further comprises an external carbon source and/or nitrogen source.
Preferably, the reagent further comprises an external carbon source of glucose.
The fusarium solani is applied to the aspect of degrading fomesafen.
The fusarium solani is applied to the aspect of serving as a soil remediation agent.
The fusarium solani is applied to the aspect of serving as a water body repairing agent.
Specifically, the preparation and detection are as follows:
the invention separates a fusarium solani from soil of a certain farm soybean field in Harbin city, and the fusarium solani is respectively identified by morphological and molecular biological methods. Morphological analysis comprises a culture method, a tabletting dyeing method and a small culture identification, a molecular biology method selects ITS (the internal transcribed spacer region) and a beta-tubulin gene (TUB), and the result shows that the ITS gene is highly homologous with Fusarium solani rDNA gene of Fusarium solani through amplification sequencing. The classification is named as: fusarium solani, depository: the China general microbiological culture Collection center (CGMCC) has the preservation number of CGMCC No.23288 and the preservation date of: 10/19/2021, preservation address is: xilu No.1 Hospital No. 3, Beijing, Chaoyang, North.
The invention researches the degradation condition of fomesafen under the co-metabolism of Fusarium solani F-F1 and nutrient substance glucose by using an inorganic salt culture medium in a laboratory, and provides a solid scientific basis for the future application of the fungus in farmland polluted soil and water.
The screening and physiological characteristic test processes of the strain are as follows:
1. materials and methods
1.1 samples, instruments, reagents and media
Sample source: the soil sample of the separated and screened strain is collected from the soil of a certain farm soybean field in Harbin.
The instrument comprises the following steps: constant temperature shaking table (gold altar wald, chang.), Centrifuge (Centrifuge 5810R, Eppendorf germany), microfiltration membrane (0.22 μm, Pall, usa), gene analyzer (3730xl DNAanalyzer, ABI, usa), capillary (50cm, 96 lane, 4331246, ABI, usa), PCR instrument (2720, ABI, usa), electrophoresis instrument (DYY-8C, sixpika, beijing), ultraviolet analyzer (UV-IV, new technology application institute, beijing), vortexer (VORTEX-5, linbel instruments ltd, jiangsu haimen), ultra high performance liquid chromatograph (H-Class, Waters, usa).
The reagent consumables are shown in table 1.
TABLE 1 summary of reagent consumables used in the experiment
Enrichment culture medium: 5g/L of peptone, 3g/L of beef extract and 5g/L of sodium chloride, adding distilled water to a constant volume, adjusting the pH value to 7.0, and autoclaving at 121 ℃ for 20 minutes before use.
Basic salt culture medium: 1.0g/L of ammonium sulfate, 1.5g/L of dipotassium hydrogen phosphate, 0.5g/L of potassium dihydrogen phosphate, 1.0g/L of sodium chloride, 0.4g/L of magnesium sulfate heptahydrate, distilled water for constant volume and hydrochloric acid for adjusting the pH value to be neutral. Autoclaved at 121 ℃ for 20 minutes before use.
Purification medium (potato dextrose agar medium): 5.0g/L of potato extract powder, 20.0g/L of glucose, 14.0g/L of agar and 5.6 +/-0.2 of pH (25 ℃). Diluting with distilled water to desired volume, subpackaging, sterilizing at 121 deg.C under high pressure for 20min, sterilizing, and shaking.
Degradation experiment culture medium: the same as basic salt culture medium.
2 method
2.1 Strain selection
Taking 10g of a soil sample from soil of a certain farm soybean field in Harbin city, adding the soil sample into a 100mL triangular flask of enrichment medium with the concentration of 50mg/L of fomesafen under the aseptic operation condition, and culturing for 5 days at the oscillation frequency of 150r/min at the temperature of 30 ℃; then transferring the mixture into a 100mL triangular flask of enrichment medium with the concentration of the fluorine-containing sulfadiazine ether being 100mg/L according to the inoculation amount of 10 percent, and continuing culturing for 5 days; then transferring the mixture into a 100mL triangular flask containing 200mg/L concentration of the fluorine-containing sulfadiazine ether enrichment medium according to the inoculation amount of 10%, and continuing culturing for 5 days; then adding 1mL of the enrichment medium bacterial liquid into a 10mL centrifuge tube, diluting the solution to a constant volume with distilled water by 10 degrees-1、10-2、10-3、10-4、10-5Respectively transferring 0.1mL of diluent to a purification culture medium plate with the concentration of the fluorine-containing sulfadiazine ether being 100mg/L, coating the plate, placing the plate in a constant-temperature incubator at 30 ℃ for 3 days, selecting single colonies with different morphological characteristics to inoculate on the purification culture medium with the concentration of the fluorine-containing sulfadiazine ether being 100mg/L, and respectively carrying out 3 times of separation and purification by adopting a plate-scribing method. After purification, the single colony strain with good growth on the plate is selected and preserved in a slant test tube with a potato glucose agar culture medium.
2.2 screening of Fomesafen functional degrading bacteria
Culturing the purified strain in an enrichment medium to logarithmic cycle (the logarithmic cycle is determined by measuring OD600 value, the logarithmic cycle is determined when the OD600 value reaches 0.9), and inoculating the strain into a basic inorganic salt culture solution with the fluorine-containing sulfadiazine ether concentration of 50mg/L by using 5% of inoculum concentration (in the experiment, the '5% inoculum concentration' refers to the volume ratio of the inoculum solution to the inoculated culture medium), wherein the inorganic salt culture medium is treated as follows: before sterilization, 2% glucose (mass fraction) was added, while the inoculum free culture medium was used as a control, and 3 replicates were treated for each sample. After the culture is finished, sucking 2mL of supernatant, adding 10mL of acetonitrile and 2g of sodium chloride, vortexing for 1min, centrifuging for 5min at 8000r/min, sucking 1mL of supernatant, and measuring the residual quantity of fomesafen by an ultra-high performance liquid chromatograph through a 0.22-micrometer filter membrane. Through screening, a fungus strain with excellent parafomesafen co-metabolism function is obtained and named as F-F1. The specific settings and results are shown in tables 2 and 3.
TABLE 2 interpretation of the significance of the different numbering processes
TABLE 3 residual amount and degradation rate of fomesafen under co-metabolism of the strains of the invention and nutrients
As shown in fig. 2, the results show that: after F-F1 inoculation, the degradation effect of fomesafen in the treatment (FM) without adding nutrient substances is higher than that of CK (the degradation rate is 4.4% after 168h culture), but the degradation rate is only 19.5% after 168h culture. After 2% Glucose (GFM) nutrient substances are added, the degradation of fomesafen in the basic salt culture medium is greatly accelerated, the residual quantity of the fomesafen is degraded to 1.7mg/L after 168 hours of culture, and the degradation rate reaches 96.2%.
2.3 method for detecting residual quantity of fomesafen serving as pesticide
2.3.1 adopting an external standard method: diluting the standard fomesafen solution into concentrations of 0.02, 0.1, 0.2, 0.5, 1, 2 and 5mg/L in sequence by using equal volume of acetonitrile and deionized water (1:1), then measuring by using an ultra-high performance liquid chromatograph, feeding 1 mu L of sample each time, processing each sample for 3 times, and taking the average value of peak areas. And taking the concentration as an abscissa and the peak area as an ordinate to make a standard curve.
2.3.2 liquid chromatography conditions of fomesafen: WatersACQUITY
BEH C18 chromatography column, 1.7 μm by 2.1mm by 100 mm; temperature of the column oven: 40 ℃; mobile phase: acetonitrile, ultrapure water, formic acid (80:20:0.1, v/v/v); wavelength: 275 nm; sample introduction amount: 5 μ L.
The residual amount of fomesafen in the culture solution is obtained through a standard curve, and the degradation rate of fomesafen is calculated through a formula 1, and the result is shown in fig. 3 and 4.
Equation 1: the degradation rate (%) - (concentration of fomesafen in the initial culture solution-residual concentration of fomesafen in the treated culture solution)/concentration of fomesafen in the initial culture solution × 100%
2.4 identification of Fomesafen function-degrading Strain F-F1
Extracting strain genome DNA: the isolated strain was cultured on potato dextrose agar for 5 days at 30 ℃ in an incubator, and then subjected to the following procedures using a RAidlab DN14 fungal extraction kit according to the instructions: scraping 0.2-0.5mg of mycelium, and placing into a 2.0ml centrifuge tube; adding 2 glass beads with diameter of 3-4MM and 100 μ l CTAB lysate, and grinding twice on a Retsch MM400 homogenizer for 2min each time; adding 400 μ l CTAB lysate preheated in 65 deg.C water bath, reversing, mixing, and adding into 65 deg.C water bath for 0.5-1 hr; adding equal volume of chloroform and isoamyl alcohol (24:1), and mixing by gentle inversion for 50 times; centrifuging at 12000rpm for 5min at room temperature, and taking supernatant to a new centrifuge tube; adding 1.5 times volume of binding solution into the supernatant, mixing well, transferring into an adsorption column, centrifuging at 12000rpm at room temperature for 30sec, and discarding waste liquid; adding 500 μ l inhibitor removing solution, centrifuging at 12000rpm at room temperature for 30sec, and discarding waste liquid; adding 600 μ l of rinsing solution, centrifuging at 12000rpm at room temperature for 30sec, and discarding the waste liquid; putting the adsorption column back into an empty collection tube, and centrifuging for 2min at the room temperature of 12000 rpm; transferring the adsorption column into a sterilized 1.5ml centrifuge tube, and drying for 5min at room temperature in an open manner; adding 50-100 μ l of elution buffer, standing at room temperature for 3-5min, centrifuging at 12000rpm for 30sec, and collecting the genomic DNA solution for immediate use or storing at-20 deg.C for later use.
Ribosomal gene (rDNA) sequence amplification sequencing: the PCR MIX buffer and primer (upstream primer ITS 1: 5'-TCCGTAGGTGAACCTGCGG-3' and downstream primer ITS 4: 5 '-TCCTCCGCTT-ATTGATATGC-3') solutions were removed and thawed at 4 ℃ or on ice for 30 minutes.
PCR amplification System (50. mu.l): dd H2O 19 μ l; 30 mul of PCR MIX enzyme buffer solution; 1 mul of upstream primer; 1 mul of downstream primer; the template 1.5. mu.l (the first 4 components are mixed into a mixture, and the mixture is subpackaged into a PCR reaction tube and then added with a DNA template).
PCR amplification reaction procedure: denaturation at 94 deg.C for 5min, melting at 94 deg.C for 0.5min, renaturation at 50 deg.C for 30s, extension at 72 deg.C for 1min, amplification for 35 cycles, extension at 72 deg.C for 7min, and storage at 4 deg.C.
And (3) PCR product purification: preparing 2% agarose gel: 2g of agarose was added to 100mL of 1 XTBE and dissolved by heating; electrophoresis voltage is 100V, and electrophoresis time is 30 min; after tapping, PCR product purification was performed according to the kit instructions.
Performing ethanol precipitation and purification on the PCR product, then applying ABI 3730XL sequencer electrophoresis on a BigDyeTrv 3.1 Cycle Seq Kit (4336921) Kit to transfer the PCR product meeting the size of a band to Tianjin Jingyang biotechnology limited for analysis and sequencing to obtain the rDNA-ITS sequence of the strain:
the rDNA-ITS sequence consists of 573 bases (bp).
2.5 colony morphology feature Observation
The F-F1 bacterial strain grows fast on a potato glucose culture medium, the diameter of a bacterial colony is 50-90 mm after the bacterial colony is cultured for 5 days at the constant temperature of 28 ℃, the hypha of a single bacterial colony is aerial hypha and is in a low flat cotton shape, grows on the substrate and flourishes. The strain is white at the edge, purple cinnamon at the middle part and light yellow at the back. No diaphragm is observed in hypha under a microscope, and the hypha is long tubular single cells. The microconidium is planted in false head form, and has ellipse, oblong, short sausage or comma shape, no partition, transparency and smoothness. The large conidium is sickle-shaped, spindle-shaped, lanceolate-shaped or cylindrical and the like, is slightly bent, has short apical cells, is slightly narrow and thin or blunt, has thick wall and has no septum; has no podocyte. The colony morphology and the microscopic morphology of the strain F-F1 are shown in FIG. 1.
2.5 identification of Strain F-F1 as a New functional Strain
Blast alignment of the sequencing results with GenBank in NCBI gave 99% homology of F-F1 to the ITS sequence of Fusarium solani. According to the morphological characteristics of the strain F-F1 and the result of rDNA-ITS sequence analysis, the strain is identified as Fusarium solani (Fusarium solani) which is named as Fusarium solani F-F1(Fusarium solani F-F1). At present, no researchers at home and abroad report that Fusarium solani (Fusarium solani) has the function of degrading fomesafen. Therefore, the strain F-F1 belongs to a new functional strain, which has been preserved in China general microbiological culture Collection center (CGMCC) at 10 and 19 months 2021 with the preservation number of CGMCC No. 23288.
Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the embodiments disclosed.
Sequence listing
<110> scientific research monitoring station for environmental protection in agricultural rural areas
<120> Fusarium solani for degrading fomesafen and application thereof
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 573
<212> DNA
<213> rDNA-ITS sequence (Unknown) of Strain F-F1
<400> 1
cctccgtagg gggtgactgc ggagggatca ttaccgagtt atacaactca tcaaccctgt 60
gaacatacct aaaacgttgc ttcggcggga acagacggcc ctgtaacaac gggccgcccc 120
cgccagagga cccctaactc tgtttttata atgtttttct gagtaaacaa gcaaataaat 180
taaaactttc aacaacggat ctcttggctc tggcatcgat gaagaacgca gcgaaatgcg 240
ataagtaatg tgaattgcag aattcagtga atcatcgaat ctttgaacgc acattgcgcc 300
cgccagtatt ctggcgggca tgcctgttcg agcgtcatta caaccctcag gcccccgggc 360
ctggcgttgg ggatcggcgg aagccccctg tgggcacacg ccgtccctca aatacagtgg 420
cggtcccgcc gcagcttcca ttgcgtagta gctaacacct cgcaactgga gagcggcgcg 480
gccatgccgt aaaacaccca acttctgaat gttgacctcg aatcaggtag gaatacccgc 540
tgaacttaag catatcaaaa ggcggaggaa ctt 573
<210> 2
<211> 19
<212> DNA
<213> upstream primer ITS1(Unknown)
<400> 2
tccgtaggtg aacctgcgg 19
<210> 3
<211> 20
<212> DNA
<213> downstream primer ITS4(Unknown)
<400> 3
tcctccgctt attgatatgc 20
Claims (10)
1. Fusarium solani for degrading fomesafen, which is characterized in that: the name of the strain is as follows: F-F1, classified and named as: fusarium solani with the preservation number of CGMCC No.23288 and the preservation date of the Fusarium solani is as follows: 10/19/2021, depository: china general microbiological culture Collection center, West Lu No.1 Hospital No. 3, Beijing, Chaoyang, the area of the republic of China.
2. The Fusarium solani degrading fomesafen of claim 1, characterized in that: the rDNA-ITS sequence of the strain F-F1 is SEQ ID NO. 1.
3. Fusarium solani degrading fomesafen according to claim 1 or 2, characterized in that: the fusarium solani F-F1 can degrade the herbicide fomesafen under the co-metabolism of the additional nutrient substances, and the degradation rate of the fomesafen can reach 96.2%.
4. The Fusarium solani degrading fomesafen of claim 3, wherein: the nutrient substances are basal salt culture medium and 2% (mass fraction) glucose;
wherein the basic salt culture medium is: 1.0g/L of ammonium sulfate, 1.5g/L of dipotassium hydrogen phosphate, 0.5g/L of potassium dihydrogen phosphate, 1.0g/L of sodium chloride, 0.4g/L of magnesium sulfate heptahydrate, distilled water for constant volume and hydrochloric acid for adjusting the pH value to be neutral; autoclaved at 121 ℃ for 20 minutes before use.
5. The fomesafen degrading agent comprising Fusarium solani degrading agent of any one of claims 1 to 3.
6. The fomesafen degrading agent according to claim 5, characterized in that: the reagent further comprises an external carbon source and/or nitrogen source.
7. The fomesafen degrading agent according to claim 5, characterized in that: the reagent also comprises an external carbon source of glucose.
8. The use of Fusarium solani of any one of claims 1 to 4 as an agent that degrades fomesafen.
9. The use of Fusarium solani of any one of claims 1 to 4 as a soil remediation agent.
10. Fusarium solani of any one of claims 1 to 4 for use as a water remediation agent.
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| CN109136097A (en) * | 2018-07-12 | 2019-01-04 | 农业部环境保护科研监测所 | The penicillium oxalicum of degradation isopropyl methoxalamine and its application |
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| CN109136097A (en) * | 2018-07-12 | 2019-01-04 | 农业部环境保护科研监测所 | The penicillium oxalicum of degradation isopropyl methoxalamine and its application |
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|---|---|---|---|---|
| CN116064296A (en) * | 2022-09-14 | 2023-05-05 | 广西大学 | Mesotrione degrading bacterium Klebsiella pasteurii CM-1 and application thereof |
| CN116064296B (en) * | 2022-09-14 | 2024-05-28 | 广西大学 | Mesotrione degrading bacterium Klebsiella pasteurii CM-1 and application thereof |
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