CN116042378B - A kind of Streptococcus suis type 2 virulence gene detection kit and its detection method - Google Patents

Abstract

The invention relates to the technical field of reagent boxes, in particular to a streptococcus suis type 2 virulence gene detection reagent box and a detection method thereof, the reagent box comprises a reagent box body, a revolving door is rotatably connected to the top of the reagent box body, an object storage groove for containing reagent materials is formed in the inner cavity of the top of the reagent box body, a T-shaped groove is formed in the inner cavity of the bottom of the reagent box body, a supporting seat is slidably connected in the T-shaped groove, when a gun head stretches into a test tube, the conical shape of the gun head contacts a V-shaped part, then the gun head slides on a second extrusion pad through a first extrusion pad, and meanwhile, an elastic potential energy is released through a compression spring, so that a command plate is driven to stretch out of a first limiting cylinder, a worker can conveniently check the command plate, whether the corresponding test tube is input with liquid or not, and the problem of excessive or missed liquid on a micropore shelf is prevented in the later stage in the process of inputting liquid can be judged through the command plate.

Description

A kind of Streptococcus suis type 2 virulence gene detection kit and its detection method

technical field

The invention relates to the technical field of kits, in particular to a detection kit for Streptococcus suis type 2 virulence gene and a detection method thereof.

Background technique

Streptococcus suis is an important zoonotic disease. The clinical symptoms of pigs after the onset are mainly meningitis, sepsis and arthritis. The existing Streptococcus suis type 2 virulence gene detection kit uses a double-antibody sandwich For enzyme-linked immunosorbent assay, add negative control, positive control, sample, and HRP-labeled detection antibody in sequence to the coated microwells pre-coated with Streptococcus suis type 2 capture antibody, and then use the substrate TMB to develop color, TMB It is converted into blue under the catalysis of peroxidase, and converted into the final yellow under the action of acid, and then use a microplate reader to measure the absorbance to determine whether the sample contains Streptococcus suis.

During the large-scale extraction experiment of Streptococcus suis, there was a problem in the use of the Streptococcus suis type 2 virulence gene detection kit: the staff put the sample or standard product into the microwells corresponding to the plate number, according to a certain sequence Input into the microwell, the sample and the standard are parallel to each other, and then use a pipette to inject the test solution into the microwell, but the existing microwell plate is transparent, and the staff needs to input various liquids into the microwell one by one Different numbers, due to the large number of microwells and the frequent input of liquid by the staff, it is necessary to close the lid of the input liquid and replace the tip on the pipette, which reduces the attention of the staff, so it is easy to cause the next time The input of the liquid in the microwell is wrong, which leads to the situation that the microwell liquid on the microwell rack is added or missed; secondly, the staff manually adds the liquid inside the pipette into the microwell, and the long-term operation cannot Keeping the pipette tip vertically at the bottom of the microwell will easily cause the liquid in the pipette tip to adhere to the side wall of the microwell, or even cause the liquid to splash out, causing liquid pollution and waste, and reducing the accuracy of the experimental quality.

Contents of the invention

In view of this, the object of the present invention is to provide a Streptococcus suis type 2 virulence gene detection kit and its detection method, which can effectively solve the problem of liquid input errors in the micropores, and the liquid in the micropores is overfilled or missed. And the liquid of the gun head is output on the side wall of the micropore, causing the problems of liquid pollution and waste.

In order to achieve the above object, the present invention provides the following technical solutions: a Streptococcus suis type 2 virulence gene detection kit, including SS-2 PCR reaction tube, SS-2 primer probe tube, SS-2 negative control tube, SS- 2 positive control tubes, SS-2 quantitative standard tubes, and a kit body, the top of the kit body is rotatably connected with a revolving door, and the top inner cavity of the kit body is provided with a reservoir for placing reagent materials slot, the bottom inner cavity of the kit body is provided with a T-shaped slot, and a support seat is slidably connected to the T-shaped slot. The inner cavity of the support seat is slidably provided with a microporous shelf. The rectangular array is equipped with a plurality of first limiting cylinders, the left side wall of the first limiting cylinder is provided with an instruction assembly, and the top of the first limiting cylinder is provided with a button for limiting the gun head. As for the position limiting component, the left and right sides of the support base are provided with take-out components for taking out the microporous shelf, and the bottom of the front end of the support base is provided with a support component.

Preferably, the command assembly includes a V-shaped piece, the left side wall of the first limiting cylinder is provided with a notch, the V-shaped piece is rotatably connected inside the notch, and the front side of the V-shaped piece is installed There is an arc frame, the bottom of the rear side of the V-shaped piece is equipped with a fixed seat, the bottom of the fixed seat is provided with a first extrusion pad, and the bottom wall of the notch is provided on one side corresponding to the first extrusion pad There is a second extrusion pad that conflicts with the first extrusion pad, a mounting plate is installed on the front end of the fixing seat, a command plate is installed on the side of the mounting plate away from the fixing seat, and the top wall of the notch is installed There is an auxiliary plate, and a compression spring is connected between the auxiliary plate and the front end of the V-shaped piece.

Preferably, a square groove is opened in the inner cavity of the first limiting cylinder and located below the second squeeze pad, and a screw is threaded on the bottom of the front end of the first limiting cylinder, and the screw extends into the square groove The inside of the square groove is equipped with a first bevel gear, the top wall of the square groove is rotatably connected with a second bevel gear meshed with the first bevel gear, and the first limit cylinder slides under the slot A nut that is fixedly connected with the second extrusion pad is connected, and a screw rod that is threadedly connected with the nut is installed on the top of the second bevel gear, and the second extrusion pad is located on the front and rear sides of the screw rod. The second limit rod extending into the inside of the square groove, and a plurality of spring telescopic rods fixedly connected with the first pressing pad are installed inside the fixed seat.

Preferably, an inclined plate is installed on the side of the auxiliary plate away from the first limiting cylinder, and a spring rod is slidably connected to the end of the inclined plate away from the first limiting cylinder, and the spring rod A pressing plate for driving the auxiliary plate to return is installed at the bottom, and a vertical pressing plate is installed on the top of the plurality of spring bars arranged vertically.

Preferably, fixed rods in a rectangular array are installed on the top of the support seat, expansion springs are sheathed on the outside of the fixed rods, the tops of the two horizontally arranged fixed rods are slidably connected with a transverse pressing plate, and the The horizontal pressing plate is squeezed on top of the vertical pressing plate.

Preferably, the limiting assembly includes a lifting cylinder screwed to the top of the first limiting cylinder, and a plurality of F-shaped supports in an annular array are installed on the top of the lifting cylinder, and a plurality of the F-shaped The two horizontal ends of the support are equipped with a second limit cylinder for limiting the gun head, and a digital limit marker is installed on the top of the lifting cylinder between adjacent F-shaped supports.

Preferably, two movable grooves are opened at the bottom of the front end of the support seat, and the bottoms of the two movable grooves are rotatably connected with a rotating block, and two connecting springs fixedly connected with the rotating block are installed in the two movable grooves The front end of the rotating block is equipped with a pressing plate, the bottom of the front end of the rotating block is equipped with a first limit rod, and the bottom of the front end of the kit body corresponds to the side of the first limit rod. Slots to fit the rods.

Preferably, the take-out assembly includes two sliding seats slidably connected to the inner side of the T-shaped slot, and the two sliding seats are respectively arranged symmetrically on the left and right sides of the support seat, and the inside of the sliding seat is rotatably connected with a take-out frame, The front end of the take-out frame protrudes from the outside of the support seat, the rear end of the take-out frame extends into the inside of the T-shaped slot and is equipped with three take-out blocks, and the side of the three take-out blocks away from the take-out frame is close to the micro On the hole frame, an inclined reset plate is installed on the rear end of the sliding seat, and a first return spring is connected between the reset plate and the take-out frame.

Preferably, the supporting assembly includes supporting feet, and two T-shaped storage slots are opened on the bottom of the front end of the supporting base, and the insides of the two T-shaped storage slots are rotationally connected with supporting feet. A connecting shaft is installed between them. A linkage slot is provided at the bottom of the support base and between two T-shaped storage slots. One end of the connecting shaft passes through the interior of the linkage slot and a driving gear is installed. The inner side of the linkage slot A rack plate meshed with the drive gear is connected through a slide rail connection, a second return spring is connected between the front end of the rack plate and the front side wall of the linkage groove, and the rear end of the rack plate is installed with a The first finger drives the board.

In addition, the present invention also provides a detection method of Streptococcus suis type 2 virulence gene detection kit, the specific steps are as follows:

S1: First, slide the support seat out of the outside, the support legs will automatically open and stand on the workbench;

S2: Place the microporous shelf on the support base, and then slide the support base out of the T-shaped slot again;

S3: When the tip of the gun extends into the bottom of the test tube, the command plate protrudes outside the first limit cylinder, so that the staff can check it;

S4: When the friction force between the first squeeze pad and the second squeeze pad gradually decreases, adjust the screw rod to drive the second squeeze pad up and down until the friction force is enough for the V-shaped piece to return;

S5: Adjust the height between the lifting cylinder and the first limit cylinder until the outlet end of the gun head extends into the bottom of the test tube, so that the gun head remains under the limit action of the two second limit cylinders Just be vertical to the test tube;

S6: When it is necessary to quickly take out the microporous shelf, pull the taking out shelf on both sides relatively, the middle part of the taking out shelf rotates in the sliding seat, and then the three taking out blocks on both sides press the microporous shelf, and then the staff can manually Take out the rack outside, and then take out the microporous rack.

Compared with prior art, the beneficial effect of the present invention is:

(1) In the present invention, when the tip of the gun is inserted into the test tube, the conical shape of the tip of the gun touches the V-shaped part, and then slides on the second squeeze pad through the first squeeze pad, and at the same time compresses the spring to release the elastic potential energy, thereby driving the instruction The plate protrudes from the first limiting cylinder, which is convenient for the staff to check the command plate. Through the command plate, it can be judged whether the corresponding test tube has input liquid, which is beneficial to avoid mistakes in the process of input liquid in the later stage, and reduces the microporous shelf. The liquid appears to add more and leak to appear.

(2) The present invention adjusts the height between the lifting cylinder and the first limiting cylinder so that the outlet end of the gun head extends into the bottom of the test tube, which is beneficial to keep the gun head between the two second limiting cylinders. It is vertical to the test tube under the limiting effect of the pipette, preventing the liquid in the pipette tip from adhering to the side wall in the micropore, and even causing the liquid to splash outside. The invention improves the accuracy of the experimental quality.

(3) The present invention facilitates the adjustment of the friction between the second squeeze pad and the first squeeze pad through the cooperation of the screw, the first bevel gear, the second bevel gear, the screw rod and the nut. In the present invention, a spring telescopic rod is also provided to reduce the impact force of the second pressing pad on the first pressing pad, thereby increasing the service life of the second pressing pad and the second pressing pad.

(4) The present invention also facilitates the staff to quickly take out the microporous shelf by setting the take-out component. At the same time, the present invention is beneficial to improve the clamping of the take-out block to the microporous shelf by setting the reset plate and the first return spring on the one hand. On the other hand, it is conducive to the clamping stability of the microwell rack, so as to facilitate the stability of the test tube placement.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the present invention;

Fig. 2 is an exploded view of the overall structure of the present invention;

Fig. 3 is a longitudinal sectional view of the first limiting cylinder of the present invention;

Fig. 4 is a partially enlarged view of A in Fig. 2;

Fig. 5 is a partially enlarged view of B in Fig. 3;

Fig. 6 is an exploded view of the first limiting cylinder and the lifting cylinder of the present invention;

Fig. 7 is a schematic diagram of another angle of the support seat of the present invention;

Fig. 8 is a partially enlarged view of C in Fig. 7;

Fig. 9 is a schematic diagram of the removal assembly of the present invention;

Fig. 10 is a schematic diagram of the support assembly of the present invention.

In the picture:

1. The body of the kit;

2. Revolving door;

3. Storage tank;

4. T-slot;

5. Support seat; 50. Movable groove; 51. Rotating block; 52. Connecting spring; 53. Pressing plate; 54. The first limit rod;

6. Microporous shelf;

7. The first limit cylinder; 70. Command assembly; 701. Notch; 702. V-shaped piece; 703. Arc frame; 704. Fixing seat; pad; 707, mounting plate; 708, auxiliary plate; 709, compression spring; 7010, square groove; 7011, screw; 7012, first bevel gear; 7013, second bevel gear; 7014, screw; 7015, screw Cap; 7016, second limit rod; 7017, spring telescopic rod; 7021, command plate; 7022, inclined plate; 7023, spring rod; 7024, pressing plate; 7025, vertical pressing plate; 7031, fixed rod; Expansion spring; 7033, horizontal pressing plate; 71, limit component; 711, lifting cylinder; 712, F-shaped support piece; 713, second limit cylinder; 714, digital limit mark;

8. Take out assembly; 81. Slide seat; 82. Take out frame; 83. Take out block; 84. Reset plate; 85. First return spring;

9. Support assembly; 91. T-shaped storage slot; 92. Support foot; 93. Connecting shaft; 94. Linkage slot; 95. Driving gear; 96. Rack plate; 97. Second return spring; 98. First finger Driver board.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Embodiment one

As shown in Figure 1-Figure 3, a Streptococcus suis type 2 virulence gene detection kit, including SS-2 PCR reaction tube, SS-2 primer probe tube, SS-2 negative control tube, SS-2 positive control Tubes, SS-2 quantitative standard tubes, also include a kit body 1, the top of the kit body 1 is rotatably connected with a revolving door 2, and the top inner cavity of the kit body 1 is provided with a storage tank 3 for placing reagent materials, The bottom inner cavity of the kit body 1 is provided with a T-shaped groove 4, and the top of the T-shaped groove 4 is provided with a linear array of test tube grooves. The T-shaped groove 4 is slidably connected with a support seat 5, and the inner cavity of the support seat 5 slides A microporous shelf 6 is provided, and a plurality of first limiting cylinders 7 are mounted on the top of the supporting seat 5 in a rectangular array. The left side wall of the first limiting cylinder 7 is provided with a command component 70, and the command component 70 includes a V-shaped Part 702, the left side wall of the first limiting cylinder 7 is provided with a notch 701, the V-shaped part 702 is rotated and connected inside the notch 701, and the front side of the V-shaped part 702 is equipped with an arc frame 703, and the V-shaped part 702 A fixed seat 704 is installed at the bottom of the rear side of the rear side, and the bottom of the fixed seat 704 is provided with a first squeeze pad 705, and the side of the bottom wall of the notch 701 corresponding to the first squeeze pad 705 is provided with a The second extrusion pad 706 that interferes, the front end of the fixed seat 704 is equipped with a mounting plate 707, the side of the mounting plate 707 away from the fixed seat 704 is equipped with a command plate 7021, the top wall of the notch 701 is equipped with an auxiliary plate 708, the auxiliary plate A compression spring 709 is connected between 708 and the front end of the V-shaped piece 702 .

When working, open the revolving door 2 to take out the reagent material inside the storage tank 3 from the storage tank 3, and then take out the support seat 5 at the bottom of the kit body 1, and then the staff arranges the test tubes one by one on the microporous shelf 6 , then put the microporous shelf 6 into the support seat 5, and at the same time, the test tube extends into the test tube groove to ensure the vertical limit of the test tube, and then when the staff inputs the liquid on the test tube, insert it at the first limit position through the tip of the gun In the cylinder 7, since the longitudinal section of the gun head is set in a conical shape, the bottom of the gun head will not contact the arc frame 703 when inserted into the inside of the first limiting cylinder 7, but as the gun head continues to extend into the inside of the test tube At this time, the top of the gun head squeezes the arc frame 703, so that the arc frame 703 drives the fixing seat 704 and the first extrusion pad 705 to slide from the second extrusion pad 706, and at the same time the compression spring 709 releases the elastic potential energy, and then drives the installation The plate 707 and the command plate 7021 rotate. When the staff is inputting the liquid, they can know whether the corresponding command plate 7021 has input the liquid according to the instructions of the command plate 7021, so that when the liquid is input again next time, they can pass the command. The plate 7021 judges whether the corresponding test tube has input liquid, which is beneficial to avoid errors in the liquid input process in the later stage, and reduces the occurrence of over-addition and omission of liquid on the microwell shelf 6 .

When it is worth noting, the instruction board 7021 is set to other attractive colors such as red, which is convenient for workers to pay more attention to the indication, and the arc frame 703 provided by the present invention is made of rubber material, so as to increase the arc The frictional force of the frame 703 and the gun head.

Embodiment two

The technical solution is basically the same as that of Embodiment 1, the difference is that, as shown in Figure 3-Figure 5, a square groove 7010 is opened in the inner cavity of the first limiting cylinder 7 and located below the second pressing pad 706, and the first limiting The bottom of the front end of the cylinder 7 is threadedly connected with a screw rod 7011. The screw rod 7011 extends into the inside of the square groove 7010 and is equipped with a first bevel gear 7012. The second bevel gear 7013, the first limit cylinder 7 is located at the bottom of the notch 701 and is slidably connected with the nut 7015 fixedly connected with the second squeeze pad 706, and the top of the second bevel gear 7013 is installed with a nut 7015 7015 threaded screw mandrel 7014, the second squeeze pad 706 is positioned at the front and back sides of screw mandrel 7014 and is equipped with the second limit bar 7016 that stretches into the square groove 7010 inside, and the inside of fixed seat 704 is installed with multiple and first The spring telescopic rod 7017 fixedly connected to the squeeze pad 705, the side of the auxiliary plate 708 away from the first limiting cylinder 7 is equipped with an inclined inclined plate 7022, and the inclined plate 7022 slides away from the end of the first limiting cylinder 7 Connected with a spring bar 7023, the bottom of the spring bar 7023 is equipped with a pressing plate 7024 for driving the auxiliary plate 708 to return, and a vertical pressing plate 7025 is installed on the top of a plurality of vertically arranged spring bars 7023, and the top of the support seat 5 is installed There are fixed rods 7031 in a rectangular array. Expansion springs 7032 are set on the outside of the fixed rods 7031. The tops of the two fixed rods 7031 arranged horizontally are slidably connected with a horizontal pressing plate 7033. The horizontal pressing plate 7033 is pressed against the vertical pressing plate 7025 on top.

When working, when the first squeeze pad 705 is constantly rubbing against the second squeeze pad 706 for a long time, the friction force between the first squeeze pad 705 and the second squeeze pad 706 is reduced, and the staff can twist the screw rod 7011, and then drive the first bevel gear 7012 and the second bevel gear 7013 to rotate mutually through the screw rod 7011, under the continuous movement of the second bevel gear 7013, the nut 7015 is raised along the top of the screw rod 7014, Furthermore, the second squeeze pad 706 is close to the first squeeze pad 705 under the limit of the second stop rod 7016, which increases the friction between the first squeeze pad 705 and the second squeeze pad 706, which facilitates Make the V-shaped piece 702 work normally. When the V-shaped piece 702 is returned, the staff can directly press the command plate 7021, and then the command plate 7021 drives the mounting plate 707 and the fixing seat 704 into the inside of the notch 701, and then the first When the compression pad 705 slides on the side wall of the second compression pad 706, the spring inside the spring telescopic rod 7017 enters a compressed state, so as to reduce the impact force caused by the second compression pad 706 to the first compression pad 705 and improve the first compression pad 705. The service life of the second squeeze pad 706 and the second squeeze pad 706.

When working, in order to reduce the fatigue of the staff pressing the command plate 7021, the present invention can drive multiple vertical spring rods 7023 to move down synchronously through the vertical pressing plate 7025, and the present invention can use two of the worker's Press the horizontal pressing plates 7033 on both sides with hands, and the expansion spring 7032 enters the compressed state, the horizontal pressing plate 7033 and the entire vertical pressing plate 7025 move down, and then the expansion spring 7032 releases the elastic potential energy, so as to press the entire vertical pressing plate 7025 The purpose of this invention is to synchronously press one command board 7021 or a plurality of vertical command boards 7021 and the entire command board 7021, thereby reducing the labor intensity of the staff for pressing the command board 7021.

Embodiment Three

The technical solution is basically the same as that of Embodiment 1, the difference is that, as shown in Figures 3-5, the top of the first limiting cylinder 7 is provided with a limiting component 71 for limiting the gun head, and the limiting component 71 includes The lifting cylinder 711 threaded on the top of the first limiting cylinder 7, the top of the lifting cylinder 711 is equipped with a plurality of F-shaped supports 712 in an annular array, and the two horizontal ends of the multiple F-shaped supports 712 are installed There is a second limiting cylinder 713 for limiting the gun head, and a digital limit marker 714 is installed on the top of the lifting cylinder 711 and between adjacent F-shaped supports 712 .

When working, the staff can twist the lifting cylinder 711 to move telescopically along the top of the first limiting cylinder 7, and adjust the length between the lifting cylinder 711 and the first limiting cylinder 7 according to the length of the gun head. , until the outlet end of the gun head extends into the bottom of the test tube, it is beneficial to keep the gun head vertical to the test tube under the limit action of the two second limiting cylinders 713, and prevent the liquid of the gun head from adhering to the micro On the side wall of the hole, even cause the liquid to splash outside, the invention improves the accuracy of the experiment quality.

It is worth noting that the present invention can twist the height of the lifting cylinder 711 according to the digital limit mark 714, so that the staff can adjust the entire lifting cylinder 711 according to the digital marking, and improve the accuracy of the height of the entire lifting cylinder 711.

Embodiment Four

The technical solution is basically the same as that of Embodiment 1, the difference is that as shown in Figure 7-8, two movable slots 50 are opened on the bottom of the front end of the support seat 5, and the bottoms of the two movable slots 50 are rotatably connected with a rotating block 51, and the two movable Two connecting springs 52 fixedly connected to the rotating block 51 are installed in the groove 50, a pressing plate 53 is installed on the front end of the rotating block 51, a first limit rod 54 is installed on the bottom of the front end of the rotating block 51, and the front end of the kit body 1 The side of the bottom corresponding to the first limiting rod 54 is provided with a hole matching the first limiting rod 54 .

When the experiment is over, the staff drives the support seat 5 into the T-shaped slot 4, and then the rotating block 51 gradually extends into the movable slot 50 inside the T-shaped slot 4, and the connecting spring 52 enters the compression spring, and then the staff presses the pressing plate 53 drives the first stop rod 54 to continue to extend into the movable groove 50, so that the first stop rod 54 can fully extend into the hole groove inside the kit body 1, so as to prevent the support seat 5 from breaking away from the kit body 1, It is convenient for the support seat 5 to be accommodated inside the reagent box body 1 .

Embodiment five

It is basically the same as the technical solution of Embodiment 1, the difference is that, as shown in Figure 2 and Figure 9, the left and right sides of the support seat 5 are provided with a take-out assembly 8 for taking out the microporous shelf 6, and the take-out assembly 8 includes a sliding connection The two sliding seats 81 inside the T-shaped groove 4 are arranged symmetrically on the left and right sides of the support seat 5 respectively. The inner rotation of the sliding seats 81 is connected with a take-out frame 82, and the front end of the take-out frame 82 is stretched out on the support. The outside of seat 5, the rear end of taking out frame 82 stretches into T-shaped groove 4 inboards and is equipped with three taking out blocks 83, and three taking out blocks 83 are close to the microporous shelf 6 away from the side of taking out frame 82, slide seat 81 The rear end of the rear end is equipped with an inclined reset plate 84, and a first return spring 85 is connected between the reset plate 84 and the take-out frame 82.

During work, when it is necessary to quickly take out the microporous shelf 6, the taking-out shelf 82 on both sides is relatively pulled, and the middle part of the taking-out shelf 82 rotates in the slide seat 81, and then the three taking-out blocks 83 on both sides carry out the microporous shelf 6. After pressing, the staff can manually take out the frame 82 out of the outside, and then take out the microporous frame 6.

It is worth noting that the present invention helps to improve the clamping strength of the removal block 83 to the micropore rack 6 by setting the return plate 84 and the first return spring 85 on the one hand, so as to quickly take out the micropore rack 6; It is conducive to the clamping stability of the microwell shelf 6, so as to facilitate the stability of test tube placement.

Embodiment six

The technical solution is basically the same as that of Embodiment 1, the difference is that, as shown in Figure 7 and Figure 10, a support assembly 9 is provided at the bottom of the front end of the support seat 5, and the support assembly 9 includes support feet 92, and two Two T-shaped storage slots 91, the inner rotation of the two T-shaped storage slots 91 are connected with supporting feet 92, and a connecting shaft 93 is installed between the two supporting feet 92. The bottom of the support seat 5 is located at the two T-shaped storage slots 91 There is a linkage groove 94 between them, and one end of the connecting shaft 93 passes through the linkage groove 94 and is equipped with a drive gear 95. The inside of the linkage groove 94 is connected with a rack plate 96 meshing with the drive gear 95 through a slide rail connection. A second return spring 97 is connected between the front end of the rack plate 96 and the front side wall of the linkage groove 94 , and a first finger driving plate 98 is installed at the rear end of the rack plate 96 .

During work, when the support seat 5 slides outside, the second return spring 97 releases the elastic potential energy and then drives the connecting shaft 93 to rotate, and then the supporting foot 92 protrudes from the inside of the T-shaped storage groove 91 under the pressure of the connecting shaft 93, so that Two support feet 92 support the support base 5 to ensure the balance and stability of the placement of the microporous shelf 6. When the drive support base 5 extends into the T-shaped slot 4, only the support feet 92 on both sides need to be inserted into the T-shaped groove 4. Type storage slot 91, and then the supporting legs 92 can be directly inserted into the inside of the kit body 1. The present invention automatically opens and stores the supporting legs 92, and the supporting legs 92 protrude from the T-shaped slot inside the kit body 1. 4 to further increase the frictional force of the support seat 5 in the T-shaped groove 4, so that the support seat 5 plays a role of stable storage in the T-shaped groove 4.

It should be noted that for the specific usage of SS-2 PCR reaction tube, SS-2 primer probe tube, SS-2 negative control tube, SS-2 positive control tube, and SS-2 quantitative standard tube, please refer to the application number How to use 202110285406.9.

In addition, the present invention also provides a detection method of Streptococcus suis type 2 virulence gene detection kit, the specific steps are as follows:

S1: Firstly, when working, when the support seat 5 slides outside, the second return spring 97 releases the elastic potential energy and drives the connecting shaft 93 to rotate, and then the supporting leg 92 extends from the inside of the T-shaped storage groove 91 under the pressure of the connecting shaft 93 out, so that the two supporting feet 92 support the supporting base 5, and ensure the placement balance and stability of the microporous shelf 6. When the driving supporting base 5 extends into the T-shaped slot 4, only the supporting feet on both sides need to be 92 stretches into the T-shaped storage groove 91, and then the supporting legs 92 directly stretch into the inside of the kit body 1 to get final product;

S2: Next, open the revolving door 2 to take out the reagent material inside the storage tank 3 from the storage tank 3, and then take out the support seat 5 at the bottom of the kit body 1, and then the staff arranges the test tubes one by one on the microwell shelf 6 Then put the microporous shelf 6 into the support seat 5, and then extend the support seat 5 into the T-shaped slot 4;

S3: When the staff inputs the liquid on the test tube, the gun head is inserted into the first limiting cylinder 7. Since the longitudinal section of the gun head is conical, the bottom of the gun head is inserted into the first limiting cylinder 7. The inside will not be in contact with the arc frame 703, but as the gun head continues to extend into the test tube, the top of the gun head squeezes the arc frame 703, so that the arc frame 703 drives the fixing seat 704 and the first extrusion pad 705 from The second pressing pad 706 slides, and at the same time, the compression spring 709 releases the elastic potential energy, and then drives the installation plate 707 and the command plate 7021 to rotate. When the staff is inputting the liquid, they can know the corresponding liquid according to the instructions of the command plate 7021. Whether the command board 7021 of the test tube has input liquid, so that when the liquid is input again next time, it can be judged through the command board 7021 whether the corresponding test tube has input liquid;

S4: When the first squeeze pad 705 is constantly rubbing against the second squeeze pad 706 for a long time, the friction between the first squeeze pad 705 and the second squeeze pad 706 is reduced, and the staff can twist the screw rod 7011 , and then the screw 7011 drives the first bevel gear 7012 and the second bevel gear 7013 to rotate mutually, under the continuous movement of the second bevel gear 7013, the nut 7015 rises along the top of the screw 7014, and then Make the second squeeze pad 706 close to the first squeeze pad 705 under the limit of the second limit rod 7016, which increases the friction between the first squeeze pad 705 and the second squeeze pad 706, so that the The V-shaped piece 702 works normally. When returning the V-shaped piece 702, the staff can directly press the command plate 7021, and then the command plate 7021 drives the mounting plate 707 and the fixing seat 704 into the notch 701, and then the first squeeze When the pressing pad 705 slides on the side wall of the second pressing pad 706, the spring inside the spring telescopic rod 7017 enters a compressed state, so as to reduce the impact force caused by the second pressing pad 706 to the first pressing pad 705;

S5: The staff can twist the lifting cylinder 711 to move telescopically along the top of the first limiting cylinder 7, and adjust the length between the lifting cylinder 711 and the first limiting cylinder 7 according to the length of the gun head. Until the outlet end of the gun head extends into the bottom of the test tube, it is enough to keep the gun head vertical to the test tube under the limiting action of the two second limiting cylinders 713;

S6: When the microporous shelf 6 needs to be taken out quickly, the taking out shelf 82 on both sides is relatively pulled, the middle part of the taking out shelf 82 rotates in the sliding seat 81, and then the three taking out blocks 83 on both sides press the microporous shelf 6 , and then the staff can manually take out the rack 82 out of the outside, and then take out the microporous rack 6.

Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other.

The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (7)

1. A detection kit for Streptococcus suis type 2 virulence gene, comprising SS-2 PCR reaction tube, SS-2 primer probe tube, SS-2 negative control tube, SS-2 positive control tube, SS-2 quantitative standard The quality control is characterized in that it also includes a kit body (1), the top of the kit body (1) is rotatably connected with a revolving door (2), and the top inner cavity of the kit body (1) is provided with a A storage tank (3) for placing reagent materials, a T-shaped slot (4) is opened in the bottom inner cavity of the kit body (1), and a support seat (5) is slidably connected to the T-shaped slot (4), The inner cavity of the support seat (5) is slidably provided with a microporous shelf (6), and the top of the support seat (5) is installed in a rectangular array with a plurality of first limit cylinders (7), the first The left side wall of the limit cylinder (7) is provided with a command component (70), and the top of the first limit cylinder (7) is provided with a limit component (71) for limiting the gun head, so The left and right sides of the support seat (5) are provided with removal components (8) for taking out the microporous shelf (6), and the support component (9) is provided at the bottom of the front end of the support seat (5); The instruction assembly (70) includes a V-shaped piece (702), the left side wall of the first limiting cylinder (7) is provided with a notch (701), and the V-shaped piece (702) is in the notch ( 701) internal rotation connection, an arc frame (703) is installed on the front side of the V-shaped piece (702), and a fixed seat (704) is installed on the rear bottom of the V-shaped piece (702), and the fixed seat The bottom of (704) is provided with a first pressing pad (705), and the side of the bottom wall of the slot (701) corresponding to the first pressing pad (705) is provided with a The second pressing pad (706) that interferes, the front end of the fixing seat (704) is equipped with a mounting plate (707), and the side of the mounting plate (707) away from the fixing seat (704) is equipped with a command plate (7021 ), the top wall of the notch (701) is equipped with an auxiliary plate (708), and a compression spring (709) is connected between the auxiliary plate (708) and the front end of the V-shaped piece (702); The limit assembly (71) includes a lifting cylinder (711) threaded on the top of the first limit cylinder (7), and a plurality of F-shaped cylinders in an annular array are installed on the top of the lifting cylinder (711). The support (712), the two horizontal ends of the multiple F-shaped supports (712) are equipped with a second limit cylinder (713) for limiting the gun head, and the lift cylinder (711) A digital limit marker (714) is installed on the top and between adjacent F-shaped supports (712). 2. A Streptococcus suis type 2 virulence gene detection kit according to claim 1, characterized in that: the inner cavity of the first limiting cylinder (7) is located in the second squeeze pad (706). A square groove (7010) is opened below, and a screw (7011) is threadedly connected to the front bottom of the first limiting cylinder (7), and the screw (7011) extends into the inside of the square groove (7010) and is installed with a second A bevel gear (7012), the top wall of the square groove (7010) is rotatably connected with a second bevel gear (7013) meshing with the first bevel gear (7012), and the first limit cylinder (7) A nut (7015) fixedly connected to the second extrusion pad (706) is slidably connected to the bottom of the notch (701), and the top of the second bevel gear (7013) is installed with a nut ( 7015) threaded screw rod (7014), the second extrusion pad (706) is located on the front and rear sides of the screw rod (7014) and installed with a second limit rod (7016) extending into the square groove (7010) , a plurality of spring telescopic rods (7017) fixedly connected with the first pressing pad (705) are installed inside the fixed seat (704). 3. A Streptococcus suis type 2 virulence gene detection kit according to claim 2, characterized in that: the side of the auxiliary plate (708) away from the first limiting cylinder (7) is installed with an inclined A type of inclined plate (7022), the end of the inclined plate (7022) away from the first limit cylinder (7) is slidably connected with a spring rod (7023), and the bottom of the spring rod (7023) is installed for driving assistance The plate (708) returns to the pressing plate (7024), and a vertical pressing plate (7025) is installed on top of the plurality of vertically arranged spring bars (7023). 4. A Streptococcus suis type 2 virulence gene detection kit according to claim 3, characterized in that: fixed rods (7031) in a rectangular array are installed on the top of the support base (5), and the fixed rods (7031) An expansion spring (7032) is sheathed on the outside of the rod (7031), and a horizontal pressing plate (7033) is slidingly connected to the top of the two fixed rods (7031) arranged horizontally, and the horizontal pressing plate (7033) is pressed on the Press vertically on top of the plate (7025). 5. A detection kit for Streptococcus suis type 2 virulence gene according to claim 4, characterized in that: two movable slots (50) are opened at the bottom of the front end of the support seat (5), and the two The bottom of the movable groove (50) is rotatably connected with a rotating block (51), and two connecting springs (52) fixedly connected with the rotating block (51) are installed in the two movable grooves (50), and the rotating block ( The front end of 51) is equipped with a pressing plate (53), the bottom of the front end of the rotating block (51) is equipped with a first limit rod (54), and the bottom of the front end of the kit body (1) corresponds to the first limit rod One side of the (54) is provided with a hole compatible with the first limit rod (54). 6. A Streptococcus suis type 2 virulence gene detection kit according to claim 1, characterized in that: the removal component (8) includes two sliding seats ( 81), the two sliding seats (81) are respectively arranged symmetrically on the left and right sides of the support seat (5), and the inner rotation of the sliding seat (81) is connected with a take-out frame (82), and the take-out frame (82 ) protrudes from the outside of the support seat (5), the rear end of the take-out frame (82) extends into the inside of the T-shaped slot (4) and is equipped with three take-out blocks (83), and the three take-out blocks ( 83) The side away from the take-out frame (82) is close to the microporous frame (6), and the rear end of the sliding seat (81) is equipped with an inclined reset plate (84), and the reset plate (84 ) and the take-out frame (82) are connected with a first return spring (85). 7. A detection kit for Streptococcus suis type 2 virulence gene according to claim 6, characterized in that: the support assembly (9) includes a support foot (92), and the bottom of the front end of the support seat (5) There are two T-shaped storage slots (91), the inner rotation of the two T-shaped storage slots (91) is connected with support feet (92), and a connecting shaft (92) is installed between the two support feet (92). 93), the bottom of the support base (5) and between the two T-shaped storage slots (91) is provided with a linkage slot (94), and one end of the connecting shaft (93) passes through the inside of the linkage slot (94) And a driving gear (95) is installed, and the inner side of the linkage groove (94) is connected with a rack plate (96) meshing with the driving gear (95) through a slide rail connection, and the rack plate (96) A second return spring (97) is connected between the front end and the front side wall of the linkage slot (94), and a first finger drive plate (98) is installed at the rear end of the rack plate (96).

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