CN116042350A

CN116042350A - Protease crude extract collection system and method

Info

Publication number: CN116042350A
Application number: CN202211721402.1A
Authority: CN
Inventors: 程汉生
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-05-02

Abstract

The invention discloses a protease crude extract collection system and a method, wherein the system comprises the following steps: the bottom of the circulation box is communicated with the bottom of the buffer tank, the first inner cavity of the circulation box is communicated with the pump inlet of the first pump body and the pump outlet of the second pump body, the pump outlet of the first pump body and the pump inlet of the second pump body are communicated with the inlet end of the first three-way valve, the first outlet end of the first three-way valve is communicated with the bottom of the fourth inner cavity of the circulation box, the second outlet end of the first three-way valve is communicated with the second outlet end of the second three-way valve, the inlet end of the second three-way valve is communicated with the bottom of the third inner cavity of the circulation box, and the circulation box is provided with a first circulation branch pipe and a second circulation branch pipe which are communicated into the inner cavities of the circulation box; the collecting box is communicated with the bottom of the second inner cavity of the circulating box and the first outlet end of the second three-way valve, and the bottom of the collecting box is communicated with the discharge end. The invention can synchronously complete the automatic collection of the two protease crude extracts, and can customize, increase and decrease the types of the collected proteases and pertinently control the processing capacity.

Description

Protease crude extract collection system and method

Technical Field

The invention relates to the technical field of protease extraction equipment, in particular to a protease crude extract collection system and a protease crude extract collection method.

Background

The male urine contains protease such as urokinase, ulinastatin and the like, the common method for extracting the protease from the urine is an adsorption method, and the urokinase is taken as an example, and the specific process flow is as follows:

s1, collecting male urine through public toilets such as schools and factories, wherein the freshness of the urine can influence the activity of urokinase, so that the urine is timely treated, the urine is collected and treated from the toilets at regular time every day, the pH value of the urine is adjusted to 9, the urine is kept stand for 1 hour, the supernatant is siphoned, and the off-white precipitate is discarded;

s2, adsorption: adjusting pH of the siphoned supernatant to 5.8-6 with 5N hydrochloric acid (HCI), adding 1% (WIV) resin, stirring and adsorbing for 1 hr, stopping stirring, naturally sinking the resin, siphoning, removing residual urine, and collecting resin. Washing the collected resin with tap water for three times, and then washing with deionized water for three times until urine is colorless;

s3, eluting: eluting for the first time, eluting with 2% ammonium hydroxide and 1M sodium chloride eluent by stirring for 1 hour according to 35% volume; a second elution, eluting for 30 minutes with the same volume of the same eluent, and starting to collect urokinase;

s4, after the activity of the finished urokinase is checked by a checking unit, the finished urokinase can be sold to a crude product purchasing manufacturer or a biochemical pharmaceutical company.

In the existing process flow for extracting protease from urine, most of the process flows are manually performed, particularly urine collection and protease adsorption are low in efficiency, and in the existing adsorption process of some extraction devices, adsorption materials are easy to sink in urine, so that the adsorption effect of the adsorption materials on the protease is limited. In addition, the current extraction equipment cannot simultaneously carry out adsorption extraction on two proteases, and the treatment capacity is not adaptively adjusted according to the actual urine collection capacity.

Disclosure of Invention

The invention aims at providing a protease crude extract collection system and a protease crude extract collection method, which can synchronously complete the automatic collection of two protease crude extracts, can customize the types of collected proteases, and can pertinently control the treatment capacity.

In order to achieve the above purpose, the invention adopts the following technical scheme:

a protease crude extract collection system comprises a buffer tank, a circulation tank, a collection tank, a first pump body, a second pump body, a first three-way valve and a second three-way valve;

the buffer tank is used for storing urine, the buffer tank is communicated with the discharge end through a first liquid discharge pipe, a first electric valve is arranged on the first liquid discharge pipe, and a first liquid level sensor is arranged on the buffer tank and used for monitoring the liquid level in the buffer tank;

the circulation box is used for protease absorption, is equipped with first slot, second slot, third slot, fourth slot, fifth slot, sixth slot and seventh slot from left to right in proper order in the inner chamber of circulation box, wherein: the partition board is inserted into the second slot, the fourth slot and the sixth slot, the inner cavity of the circulation box is sequentially divided into a first inner cavity, a second inner cavity, a third inner cavity and a fourth inner cavity, or the partition board is inserted into the first slot, the third slot, the fourth slot, the fifth slot and the seventh slot, the inner cavity of the circulation box is sequentially divided into a first cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity and a second cavity, or the partition board is inserted into the first slot, the third slot, the fourth slot and the fifth slot, and the inner cavity of the circulation box is sequentially divided into a first cavity, a first inner cavity, a second inner cavity, a third inner cavity and a fourth inner cavity;

the circulating box is lower than the buffer tank, the bottom of a first inner cavity of the circulating box is communicated with the bottom of the buffer tank through a first liquid inlet pipe, a second electric valve is arranged on the first liquid inlet pipe, the bottom of the first inner cavity of the circulating box is communicated with the discharge end through a second liquid discharge pipe, a third electric valve is arranged on the second liquid discharge pipe, a first automatic feeding device and a second automatic feeding device are arranged above the circulating box and are respectively used for feeding different adsorption materials into a second inner cavity and a third inner cavity of the circulating box, and a second liquid level sensor is arranged on the circulating box and is used for monitoring the liquid level in the circulating box;

the first inner cavity of the circulation box is communicated with the pump inlet of the first pump body and the pump outlet of the second pump body, the pump outlet of the first pump body and the pump inlet of the second pump body are communicated with the inlet end of the first three-way valve, the first outlet end of the first three-way valve is communicated with the bottom of the fourth inner cavity of the circulation box, the second outlet end of the first three-way valve is communicated with the second outlet end of the second three-way valve, the inlet end of the second three-way valve is communicated with the bottom of the third inner cavity of the circulation box, a first circulation branch pipe communicated to the second inner cavity of the circulation box is arranged on the circulation box, a spray header is arranged at one end of the first circulation branch pipe, which is positioned in the second inner cavity, of the first circulation branch pipe is communicated with the second circulation branch pipe, a spray header is arranged at one end of the second circulation branch pipe, which is positioned in the third inner cavity, of the second circulation branch pipe is communicated with the first outlet end of the first three-way valve, and a fifth electric valve is arranged on the circulation branch pipe;

the collecting box is used for collecting adsorption materials and is lower than the circulating box, the collecting box is communicated with the bottom of a second inner cavity of the circulating box through a first collecting branch pipe, a sixth electric valve is arranged on the first collecting branch pipe, the collecting box is communicated with a first outlet end of a second three-way valve through a second collecting branch pipe, a seventh electric valve is arranged on the second collecting branch pipe, a first filtering device for separating urine discharged from the second inner cavity of the circulating box to the collecting box to the corresponding adsorption materials and a second filtering device for separating urine discharged from a third inner cavity of the circulating box to the collecting box to the corresponding adsorption materials are arranged in the collecting box, and the bottom of the collecting box is communicated with the discharge end;

when two proteases are collected through two adsorption materials, the partition boards inserted in the second slot, the fourth slot and the sixth slot are all screen boards, or the partition boards inserted in the first slot and the seventh slot are all solid boards, the partition boards inserted in the third slot, the fourth slot and the fifth slot are all screen boards, the inlet end of the first three-way valve is communicated with the first outlet end, and the inlet end of the second three-way valve is communicated with the first outlet end;

when the protease is collected through the adsorption material, the partition boards inserted in the second slot and the fourth slot are screen boards, the partition board inserted in the sixth slot is a solid board, or the partition boards inserted in the first slot and the fifth slot are solid boards, the partition boards inserted in the third slot and the fourth slot are screen boards, the inlet end of the first three-way valve is communicated with the second outlet end, and the inlet end of the second three-way valve is communicated with the second outlet end.

Further, still include the pre-buffer tank, the pre-buffer tank passes through second feed liquor pipe and collects the district intercommunication, and is equipped with eighth motorised valve on the second feed liquor pipe, the pre-buffer tank passes through third feed liquor pipe and buffer tank intercommunication, and is equipped with the third pump body on the third feed liquor pipe, the pre-buffer tank passes through third fluid-discharge tube and discharges the end intercommunication, and is equipped with ninth motorised valve on the third fluid-discharge tube, be equipped with third level sensor on the pre-buffer tank for the liquid level in the monitoring pre-buffer tank.

Further, still include the washing of being responsible for with the water source intercommunication, be equipped with tenth motorised valve and fourth pump body on the washing is responsible for, and it all communicates with four inner chambers and the collecting box of pre-buffer tank, circulation case through wasing the branch pipe, all is equipped with eleventh motorised valve on each wasing the branch pipe, and is located the container one end and all is equipped with the shower head.

Further, still including a plurality of washings casees that are used for storing different cleaners, each washs the case and all communicates with wasing the person in charge through annotating the liquid branch pipe, and annotates and be equipped with fifth pump body and first check valve on the liquid branch pipe in proper order by wasing the case to wasing the person in charge, first check valve is used for the separation washs the flow direction of being responsible for to wasing the case.

Further, the first liquid discharge pipe, the second liquid discharge pipe and the third liquid discharge pipe are all communicated to the cleaning main pipe, the communication position is located at one side of the water outlet end of the tenth electric valve, the first discharge branch pipe is communicated with the discharge end, and the twelfth electric valve is arranged on the first discharge branch pipe.

Further, the cleaning main pipe is communicated with the collecting area through an external cleaning branch pipe, a thirteenth electric valve is arranged on the external cleaning branch pipe, the collecting area is communicated with the discharging end through a second discharging branch pipe, and a fourteenth electric valve is arranged on the second discharging branch pipe.

Further, the first filtering device is a first filtering net bag sleeved on one end of the first collecting branch pipe communicated with the collecting box, and the second filtering device is a second filtering net bag sleeved on one end of the second collecting branch pipe communicated with the collecting box.

A method for collecting protease crude extract adopts the system and comprises the following steps:

when two proteases were collected by the two adsorption materials, the following steps were performed: injecting urine in the buffer tank into the circulation tank through the first liquid inlet pipe; then, one kind of adsorption material is put into the second inner cavity of the circulation box through the first automatic feeding device, the other kind of adsorption material is put into the third inner cavity of the circulation box through the second automatic feeding device, and meanwhile, the following two steps are alternately carried out according to the first preset time: injecting urine in the first inner cavity of the circulation box into the third inner cavity and the fourth inner cavity of the circulation box through the first pump body, and injecting urine in the fourth inner cavity of the circulation box into the first inner cavity and the second inner cavity of the circulation box through the second pump body; finally, injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collecting box through the first collecting branch pipe, injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collecting box through the second collecting branch pipe, enabling the urine to directly flow into the discharge end, and enabling the two adsorbing materials to be respectively left in the first filtering device and the second filtering device;

when a protease is collected by an adsorbent material, the following steps are performed: injecting urine in the buffer tank into the circulation tank through the first liquid inlet pipe; then, an adsorption material is put into a second inner cavity of the circulation box through a first automatic feeding device, and the following two steps are alternately carried out according to a second preset time: injecting urine in the first inner cavity of the circulation box into the third inner cavity of the circulation box through the first pump body, and injecting urine in the third inner cavity of the circulation box into the first inner cavity and the second inner cavity of the circulation box through the second pump body; finally, the mixture of the urine and the corresponding adsorbing material in the second inner cavity of the circulation box is injected into the collecting box through the first collecting branch pipe, the urine directly flows into the discharging end, and the adsorbing material is left in the first filtering device.

Further, when two proteases are collected by the two adsorption materials, the method further comprises the following steps: injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collection box through the first collection branch pipe, injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collection box through the second collection branch pipe, and pumping clear water to the corresponding spray heads through the cleaning main pipe and the cleaning branch pipes in the first inner cavity, the second inner cavity, the third inner cavity and the fourth inner cavity of the circulation box through the fourth pump body to spray out, and flushing the residual adsorbing materials on the screen plate;

when a protease is collected by an adsorption material, the method further comprises the following steps: after the mixture of urine and corresponding adsorbing materials in the second inner cavity of the circulation box is injected into the collection box through the first collection branch pipe, clean water is pumped to the corresponding spray head to spray through the fourth pump body and the cleaning main pipe and the cleaning branch pipes in the first inner cavity, the second inner cavity and the third inner cavity of the circulation box, and residual adsorbing materials on the screen plate are washed.

Further, a second one-way valve is arranged between the fourth electric valve and the spray header on the first circulating branch pipe and used for blocking the flow direction from the spray header to the fourth electric valve, a third one-way valve is arranged between the fifth electric valve and the spray header on the second circulating branch pipe and used for blocking the flow direction from the spray header to the fifth electric valve.

The beneficial effects of the invention are as follows:

the invention can synchronously complete the automatic collection of the two protease crude extracts, and carry out system cleaning in the production process and after the production is completed, and can customize, increase and decrease the types of the collected proteases and pertinently control the treatment capacity based on the variable multi-chamber structure of the circulation box and the alternative circulation mode, and simultaneously, in the protease adsorption process, the adsorption material can be ensured to be always suspended in urine, thereby improving the adsorption rate.

Drawings

FIG. 1 is a schematic diagram showing the communication structure of a crude protease extract collecting system of the present invention (partition plates inserted into the second, fourth and sixth slots);

FIG. 2 is a schematic diagram showing the communication structure of the crude protease extract collecting system of the present invention (partition board inserted into first, third, fourth, fifth and seventh slots);

FIG. 3 is a schematic diagram showing the communication structure of the crude protease extract collecting system of the present invention (partition board inserted into first, third, fourth and fifth slots);

FIG. 4 is a schematic view (top view) of the circulation tank of the present invention;

FIG. 5 is a schematic diagram of the communication structure of the pre-buffer tank of the present invention;

FIG. 6 is a schematic diagram of a communication structure of a buffer tank according to the present invention;

FIG. 7 is a schematic illustration of the communication structure of the circulation tank of the present invention;

FIG. 8 is a schematic diagram of the communication structure of the collecting box of the present invention;

fig. 9 is a schematic diagram of a communication structure of the cleaning tank of the present invention.

Labeling and describing: 1. pre-buffer tank, 2, buffer tank, 3, circulation tank, 3-1, first slot, 3-2, second slot, 3-3, third slot, 3-4, fourth slot, 3-5, fifth slot, 3-6, sixth slot, 3-7, seventh slot, 4, collection tank, 5, first automatic feeder, 6, second automatic feeder, 7, cleaning main pipe, 8, tenth electric valve, 9, fourth pump body, 10, external cleaning branch pipe, 11, thirteenth electric valve, 12, second discharge branch pipe, 13, fourteenth electric valve, 14, first discharge branch pipe, 15, twelfth electric valve, 16, second inlet pipe, 17, eighth electric valve, 18, ninth electric valve, 19, third liquid discharge pipe, 20, cleaning branch pipe, 21, eleventh electric valve, 22, overflow pipe, 23, third inlet pipe, 24, third pump body, 25, first drain pipe, 26, first electric valve, 27, first inlet pipe, 28, second electric valve, 29, second drain pipe, 30, third electric valve, 31, second pump body, 32, first pump body, 33, first three-way valve, 34, second three-way valve, 35, second circulation branch pipe, 36, fifth electric valve, 37, third check valve, 38, first circulation branch pipe, 39, fourth electric valve, 40, second check valve, 41, first collection branch pipe, 42, sixth electric valve, 43, second collection branch pipe, 44, seventh electric valve, 45, first filter mesh bag, 46, second filter mesh bag, 47, purge tank, 48, liquid injection branch pipe, 49, fifth pump body, 50, first check valve.

Detailed Description

The invention is further described below with reference to the accompanying drawings.

Example 1:

referring to fig. 1-9, a protease crude extract collecting system comprises a buffer tank 2, a circulation tank 3, a collecting tank 4, a first pump body 32, a second pump body 31, a first three-way valve 33 and a second three-way valve 34.

The buffer tank 2 is used for urine storage, urine coming from the collection area. The buffer tank 2 is communicated with the discharge end through a first liquid discharge pipe 25, a first electric valve 26 is arranged on the first liquid discharge pipe 25 and used for controlling the opening and closing of the first liquid discharge pipe 25, and a first liquid level sensor is arranged on the buffer tank 2 and used for monitoring the liquid level in the buffer tank 2.

The collecting area refers to a urinal or other devices capable of collecting urine, and the discharge end is a sewage disposal facility such as a sewer, a septic tank and the like.

Due to the wide variety of urine collection scenarios, it is sometimes not possible for the collection system to have buffer tank 2 below each collection zone. Therefore, it is preferable that the collecting system further comprises a pre-buffer tank 1, the pre-buffer tank 1 is communicated with the collecting area through a second liquid inlet pipe 16, an eighth electric valve 17 is arranged on the second liquid inlet pipe 16 and used for controlling the opening and closing of the second liquid inlet pipe 16, the pre-buffer tank 1 is communicated with the buffer tank 2 through a third liquid inlet pipe 23, a third pump body 24 is arranged on the third liquid inlet pipe 23, the pre-buffer tank 1 is communicated with the discharging end through a third liquid outlet pipe 19, a ninth electric valve 18 is arranged on the third liquid outlet pipe 19 and used for controlling the opening and closing of the third liquid outlet pipe 19, and a third liquid level sensor is arranged on the pre-buffer tank 1 and used for monitoring the liquid level in the pre-buffer tank 1.

Specifically, urine in the collecting area enters the pre-buffer tank 1 through the second liquid inlet pipe 16, and when the liquid level in the pre-buffer tank 1 reaches a preset value, the urine is pumped to the buffer tank 2 through the third liquid inlet pipe 23 by the third pump body 24. Typically, pre-buffer tank 1 is smaller in volume and buffer tank 2 is larger in volume.

The circulation box 3 is used for protease adsorption, and the inner cavity of the circulation box 3 is sequentially provided with a first slot 3-1, a second slot 3-2, a third slot 3-3, a fourth slot 3-4, a fifth slot 3-5, a sixth slot 3-6 and a seventh slot 3-7 from left to right, wherein:

the second slot 3-2, the fourth slot 3-4 and the sixth slot 3-6 are inserted with a baffle plate, and the inner cavity of the circulation box 3 is divided into a first inner cavity, a second inner cavity, a third inner cavity and a fourth inner cavity in sequence, as shown in figure 1;

or the first slot 3-1, the third slot 3-3, the fourth slot 3-4, the fifth slot 3-5 and the seventh slot 3-7 are inserted with a baffle plate, and the inner cavity of the circulation box 3 is divided into a first cavity, a first inner cavity, a second inner cavity, a third inner cavity, a fourth inner cavity and a second cavity in sequence, as shown in figure 2;

or the first slot 3-1, the third slot 3-3, the fourth slot 3-4 and the fifth slot 3-5 are inserted with a baffle plate, and the inner cavity of the circulation box 3 is divided into a first cavity, a first inner cavity, a second inner cavity, a third inner cavity and a fourth inner cavity in sequence, as shown in figure 3.

In the embodiment, the partition plates are inserted into the second slot 3-2, the fourth slot 3-4 and the sixth slot 3-6, and the area in the circulation box 3 is equally divided into 4 inner cavities; the partition plates are inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4, the fifth slot 3-5 and the seventh slot 3-7, and the area between the first slot 3-1 and the seventh slot 3-7 in the circulation box 3 is equally divided into 4 inner cavities; the partition plates are inserted into the first slot 3-1, the third slot 3-3, the fourth slot 3-4 and the fifth slot 3-5, and the area between the first slot 3-1 and the fifth slot 3-5 in the circulation box 3 is equally divided into 3 inner cavities.

The circulation box 3 is lower than the buffer tank 2, so that urine in the buffer tank 2 can automatically flow into the circulation box 3. The bottom of the first inner cavity of the circulation box 3 is communicated with the bottom of the buffer tank 2 through a first liquid inlet pipe 27, a second electric valve 28 is arranged on the first liquid inlet pipe 27 and used for controlling the opening and closing of the first liquid inlet pipe 27, the bottom of the first inner cavity of the circulation box 3 is communicated with a discharge end through a second liquid outlet pipe 29, and a third electric valve 30 is arranged on the second liquid outlet pipe 29 and used for controlling the opening and closing of the second liquid outlet pipe 29. The first automatic feeding device 5 and the second automatic feeding device 6 are arranged above the circulation box 3 and are respectively used for feeding different adsorption materials into the second inner cavity and the third inner cavity of the circulation box 3, for example, when the target collecting substances are urokinase and ulinastatin, the adsorption materials are respectively silica gel particles and chitin. The circulation tank 3 is provided with a second liquid level sensor for monitoring the liquid level in the circulation tank 3.

The first inner cavity of the circulation box 3 is communicated with the pump inlet of the first pump body 32 and the pump outlet of the second pump body 31, the pump outlet of the first pump body 32 and the pump inlet of the second pump body 31 are communicated with the inlet end of the first three-way valve 33, the first outlet end of the first three-way valve 33 is communicated with the bottom of the fourth inner cavity of the circulation box 3, the second outlet end of the first three-way valve 33 is communicated with the second outlet end of the second three-way valve 34, the inlet end of the second three-way valve 34 is communicated with the bottom of the third inner cavity of the circulation box 3, a first circulation branch pipe 38 communicated to the second inner cavity of the circulation box 3 is arranged on the circulation box 3, a spray head is arranged at one end of the first circulation branch pipe 38 positioned in the second inner cavity, the other end of the first circulation branch pipe 38 is communicated with the pump outlet of the second pump body 31, a fourth electric valve 39 is arranged on the circulation box 3 and used for controlling the opening and closing of the first circulation branch pipe 38, a second circulation branch pipe 35 communicated to the third inner cavity of the second circulation box is arranged on the circulation box 3, and the second circulation branch pipe 35 is communicated with the first outlet of the first three-way valve 33 and the fifth circulation valve is used for controlling the opening and closing of the first circulation branch pipe 35.

It is necessary that both the pump inlet side of the first pump body 32 and the pump inlet side of the second pump body 31 are provided with filters.

The collecting box 4 is used for collecting the adsorption material, and the collecting box 4 is lower than the circulating box 3, so that urine in the circulating box 3 can automatically flow into the collecting box 4. The collecting box 4 is communicated with the bottom of the second inner cavity of the circulating box 3 through a first collecting branch pipe 41, a sixth electric valve 42 is arranged on the first collecting branch pipe 41 and used for controlling the opening and closing of the first collecting branch pipe 41, the collecting box 4 is communicated with the first outlet end of the second three-way valve 34 through a second collecting branch pipe 43, a seventh electric valve 44 is arranged on the second collecting branch pipe 43 and used for controlling the opening and closing of the second collecting branch pipe 43, a first filtering device for separating urine discharged from the second inner cavity of the circulating box 3 into the collecting box 4 from corresponding adsorbing materials and a second filtering device for separating urine discharged from the third inner cavity of the circulating box 3 into the collecting box 4 from corresponding adsorbing materials are arranged in the collecting box 4, and the bottom of the collecting box 4 is communicated with the discharging end.

Specifically, the first filtering device is a first filtering net bag 45 sleeved on one end of the first collecting branch pipe 41 communicated with the collecting box 4, and the second filtering device is a second filtering net bag 46 sleeved on one end of the second collecting branch pipe 43 communicated with the collecting box 4. After the production is completed, the first filter mesh bag 45 and/or the second filter mesh bag 46 may be directly removed.

In the above technical scheme, the pre-buffer tank 1, the buffer tank 2, the circulation tank 3 and the collection tank 4 are all communicated with the discharge end through the overflow pipe 22, so as to achieve the purpose of preventing overflow. In particular, the overflow pipe 22 on the circulation tank 3 should be careful about the connection position, avoiding that the overflow effect cannot be achieved due to the change of the partition plate.

According to different collection requirements, the system is set as follows:

when two proteases are collected through two adsorption materials, the partition boards inserted in the second slot 3-2, the fourth slot 3-4 and the sixth slot 3-6 are screen boards to adapt to larger treatment capacity, or the partition boards inserted in the first slot 3-1 and the seventh slot 3-7 are solid boards, the partition boards inserted in the third slot 3-3, the fourth slot 3-4 and the fifth slot 3-5 are screen boards to adapt to smaller treatment capacity, the inlet end of the first three-way valve 33 is communicated with the first outlet end, and the inlet end of the second three-way valve 34 is communicated with the first outlet end;

when collecting a protease by an adsorption material, the partition boards inserted in the second slot 3-2 and the fourth slot 3-4 are all screen boards, the partition board inserted in the sixth slot 3-6 is a solid board to adapt to larger treatment capacity, or the partition boards inserted in the first slot 3-1 and the fifth slot 3-5 are all solid boards, the partition boards inserted in the third slot 3-3 and the fourth slot 3-4 are all screen boards to adapt to smaller treatment capacity, the inlet end of the first three-way valve 33 is communicated with the second outlet end, and the inlet end of the second three-way valve 34 is communicated with the second outlet end.

Specifically, the method for collecting the protease crude extract adopts the system and comprises the following steps:

when two proteases were collected by the two adsorption materials, the following steps were performed: injecting urine in the buffer tank 2 into the circulation tank 3 through the first liquid inlet pipe 27; then, one adsorption material is put into the second inner cavity of the circulation box 3 through the first automatic feeding device, and the other adsorption material is put into the third inner cavity of the circulation box 3 through the second automatic feeding device, and at the same time, the following two steps are alternately performed according to the first preset time: injecting urine in the first inner cavity of the circulation tank 3 into the third inner cavity and the fourth inner cavity thereof through the first pump body 32, and injecting urine in the fourth inner cavity of the circulation tank 3 into the first inner cavity and the second inner cavity thereof through the second pump body 31; finally, the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation tank 3 is injected into the collecting tank 4 through the first collecting branch pipe 41, the mixture of the urine and the corresponding adsorbing materials in the third inner cavity of the circulation tank 3 is injected into the collecting tank 4 through the second collecting branch pipe 43, the urine directly flows into the discharge end, and the two adsorbing materials are respectively left in the first filtering device and the second filtering device;

when a protease is collected by an adsorbent material, the following steps are performed: injecting urine in the buffer tank 2 into the circulation tank 3 through the first liquid inlet pipe 27; then, an adsorption material is put into the second inner cavity of the circulation box 3 through the first automatic feeding device, and the following two steps are alternately performed according to the second preset time: injecting the urine in the first inner cavity of the circulation tank 3 into the third inner cavity thereof through the first pump body 32, and injecting the urine in the third inner cavity of the circulation tank 3 into the first inner cavity and the second inner cavity thereof through the second pump body 31; finally, the mixture of urine and the corresponding absorbent material in the second inner chamber of the circulation tank 3 is injected into the collection tank 4 through the first collection branch 41, urine will flow directly into the discharge end, and the absorbent material will remain in the first filter device.

In order to impact the bottom adsorption material, the spray heads arranged on the first circulating branch pipe 38 and the second circulating branch pipe 35 are arranged at the bottoms of the respective inner cavities.

In the above method for collecting the crude protease extract, urine circulates between the inner chambers of the circulation tank 3, and since there is a delay in opening and closing the fourth and fifth

electric valves

39 and 36, it is preferable that a second check valve 40 is provided between the fourth electric valve 39 and the shower head on the first circulation branch pipe 38, the second check valve 40 is used for blocking the flow direction from the shower head to the fourth electric valve 39, a third check valve 37 is provided between the fifth electric valve 36 and the shower head on the second circulation branch pipe 35, and the third check valve 37 is used for blocking the flow direction from the shower head to the fifth electric valve 36. According to the above design, the fourth and fifth electrically operated

valves

39 and 36 can be kept normally open during the circulation of urine between the respective chambers of the circulation tank.

In addition, since the first liquid inlet pipe 27, the first outlet end of the first three-way valve 33, the inlet end of the second three-way valve 34 and the pump outlet of the second pump body 31 are all communicated with the bottoms of the corresponding inner cavities of the circulation tank 3, and the spray heads arranged on the first circulation branch pipe 38 and the second circulation branch pipe 35 are all arranged at the bottoms of the respective inner cavities, bubbles generated in the process of circulating urine among the inner cavities of the circulation tank 3 are obviously reduced.

Example 2:

referring to fig. 1-9, on the basis of embodiment 1, the cleaning device further comprises a main cleaning pipe 7 communicated with a water source, a tenth electric valve 8 and a fourth pump body 9 are arranged on the main cleaning pipe 7, the tenth electric valve 8 is used for controlling the opening and closing of the main cleaning pipe 7, and is communicated with the four inner cavities of the pre-buffer tank 1, the buffer tank 2 and the circulation tank 3 and the collecting tank 4 through cleaning branch pipes 20, eleventh electric valves 21 are arranged on each cleaning branch pipe 20 and used for controlling the opening and closing of each cleaning branch pipe 20, and a spray header is arranged at one end in the container.

When the pre-buffer tank 1, the buffer tank 2, the circulation tank 3 and the collecting tank 4 need to be cleaned independently, clean water is pumped to the corresponding spray header for spraying through the cleaning main pipe 7 and the corresponding cleaning branch pipe 20 by the fourth pump body 9.

To achieve complete cleaning of the containers, the shower heads provided on each cleaning manifold 20 should be at the top of the respective interior chamber.

When the system requires cleaning as a whole, i.e. the pre-buffer tank 1, the buffer tank 2, the circulation tank 3 and the collection tank 4 and the pipes between the respective containers (including the parts on the pipes) all need to be cleaned, the following steps are performed:

pumping clear water to the pre-buffer tank 1 through a cleaning main pipe 7 and a cleaning branch pipe 20 in the pre-buffer tank 1 by a fourth pump body 9, and cleaning the pre-buffer tank 1 and corresponding pipelines;

the third pump body 24 pumps the cleaning water in the pre-buffer tank 1 to the buffer tank 2 through the third liquid inlet pipe 23, and cleans the buffer tank 2 and corresponding pipelines;

injecting the cleaning water in the buffer tank 2 into the circulation tank 3 through the first liquid inlet pipe 27;

in the circulation box 3, when two proteases are collected by the two adsorption materials, the following two steps are alternately performed according to a third preset time: the cleaning water in the first inner cavity of the circulation box 3 is injected into the third inner cavity and the fourth inner cavity of the circulation box 3 through the first pump body 32, the cleaning water in the fourth inner cavity of the circulation box 3 is injected into the first inner cavity and the second inner cavity of the circulation box 3 through the second pump body 31, the circulation box 3 and corresponding pipelines are cleaned, after the circulation box 3 is cleaned, the cleaning water in the circulation box 3 is injected into the collection box 4 through the first collection branch pipe 41 and the second collection branch pipe 43, the collection box 4 and the corresponding pipelines are cleaned, and the cleaning water in the collection box 4 directly flows into a discharge end;

when a protease is collected by an adsorption material, the following two steps are alternately performed according to a fourth preset time: the first pump body 32 is used for injecting the cleaning water in the first inner cavity of the circulation tank 3 into the third inner cavity of the circulation tank 3, the second pump body 31 is used for injecting the cleaning water in the third inner cavity of the circulation tank 3 into the first inner cavity and the second inner cavity of the circulation tank 3, the circulation tank 3 and corresponding pipelines are cleaned, after the circulation tank 3 is cleaned, the cleaning water in the circulation tank 3 is injected into the collecting tank 4 through the first collecting branch pipe 41, the collecting tank 4 and the corresponding pipelines are cleaned, and the cleaning water in the collecting tank 4 directly flows into the discharge end.

The collecting area and the corresponding pipelines are also necessary to be cleaned, so that the cleaning main pipe 7 is communicated with the collecting area through the external cleaning branch pipe 10, the thirteenth electric valve 11 is arranged on the external cleaning branch pipe 10 and used for controlling the opening and closing of the external cleaning branch pipe 10, the collecting area is communicated with the discharge end through the second discharge branch pipe 12, and the fourteenth electric valve 13 is arranged on the second discharge branch pipe 12 and used for controlling the opening and closing of the second discharge branch pipe 12.

Specifically, when the collecting area needs to be cleaned individually, clean water is pumped to the corresponding collecting area through the cleaning main pipe 7 and the external cleaning branch pipe 10 by the fourth pump body 9, and the cleaning water of the collecting area flows into the discharge end through the second discharge branch pipe 12.

Among the above-mentioned technical scheme, still including a plurality of washing cases 47 that are used for storing different cleaning agents, each washing case 47 all communicates with washing main pipe 7 through annotating liquid branch pipe 48, and annotates and be equipped with fifth pump body 49 and first check valve 50 on the liquid branch pipe 48 in proper order by washing case 47 to washing main pipe 7, first check valve 50 is used for the separation washing main pipe 7 to the flow direction of washing case 47.

Specifically, when the system needs to be cleaned in whole depth, cleaning agent is added into clear water when the system is cleaned in whole; when the collecting area needs to be cleaned in a single depth, cleaning agent is added into the clean water when the collecting area is cleaned in a single depth.

It is necessary that the system is subjected to a deep cleaning at least once more, and likewise, the collection area is subjected to a deep cleaning at least once more, and then to a single cleaning. Typically, deep cleaning is performed late at night.

Wherein the water source is tap water or a water tank, and the like, and the cleaning agent is selected according to the cleaning requirement.

As one embodiment, the first drain pipe 25, the second drain pipe 29 and the third drain pipe 19 are all communicated to the main cleaning pipe 7, the communicating position is located at the water outlet end side of the tenth electric valve 8, and the communicating position is communicated with the discharge end through the first discharge branch pipe 14, and the first discharge branch pipe 14 is provided with a twelfth electric valve 15 for controlling the opening and closing of the first discharge branch pipe 14.

Specifically, according to the above design, when the pre-buffer tank 1 needs to be cleaned separately, clean water can be injected from the bottom upwards through the third liquid discharge pipe 19; when the buffer tank 2 needs to be cleaned independently, clean water can be injected from the bottom upwards through the first liquid discharge pipe 25; when the circulation tank 3 needs to be cleaned separately, clean water can be injected from the bottom upwards through the second liquid discharge pipe 29.

It should be noted that the above-mentioned various cleaning methods should be selected according to actual production requirements.

As another embodiment, the method for collecting the crude protease extract adopts the system, and further comprises the following steps:

when two proteases are collected by the two adsorption materials, the mixture of urine and the corresponding adsorption material in the second inner cavity of the circulation box 3 is injected into the collection box 4 through the first collection branch pipe 41, and after the mixture of urine and the corresponding adsorption material in the second inner cavity of the circulation box 3 is injected into the collection box 4 through the second collection branch pipe 43, clear water is pumped to the corresponding spray heads through the fourth pump body 9 through the cleaning main pipe 7 and the cleaning branch pipes 20 in the first inner cavity, the second inner cavity, the third inner cavity and the fourth inner cavity of the circulation box 3, residual adsorption material on the screen plate is washed, and the clear water and the residual adsorption material are injected into the collection box 4 through the first collection branch pipe 41 and the second collection branch pipe 43;

when collecting a protease by an adsorption material, after injecting the mixture of urine and the corresponding adsorption material in the second inner cavity of the circulation box 3 into the collection box 4 through the first collection branch pipe 41, clean water is pumped to the corresponding spray heads through the cleaning main pipe 7 and the cleaning branch pipes 20 in the first inner cavity, the second inner cavity and the third inner cavity of the circulation box 3 by the fourth pump body 9, residual adsorption material on the screen plate is washed, and the clean water and the residual adsorption material are injected into the collection box 4 through the first collection branch pipe 41.

In general, the invention can synchronously complete the automatic collection of two protease crude extracts, and carry out system cleaning in the production process and after the production is completed, and the invention can customize, increase and decrease the types of collected proteases and pertinently control the treatment capacity based on the variable multi-chamber structure of the circulation box 3 and the alternative circulation mode, and simultaneously can ensure that the adsorption material is always suspended in urine in the protease adsorption process, thereby improving the adsorption rate.

Of course, the above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, so that all equivalent modifications made in the principles of the present invention are included in the scope of the present invention.

Claims

1. A protease crude extract collection system characterized by: the device comprises a buffer tank, a circulation tank, a collecting box, a first pump body, a second pump body, a first three-way valve and a second three-way valve;

2. A crude protease enzyme extract collection system according to claim 1 wherein: still including the pre-buffer tank, the pre-buffer tank passes through second feed liquor pipe and collection district intercommunication, and is equipped with eighth motorised valve on the second feed liquor pipe, the pre-buffer tank passes through third feed liquor pipe and buffer tank intercommunication, and is equipped with the third pump body on the third feed liquor pipe, the pre-buffer tank passes through third fluid-discharge tube and discharge end intercommunication, and is equipped with ninth motorised valve on the third fluid-discharge tube, is equipped with third level sensor on the pre-buffer tank for the liquid level in the monitoring pre-buffer tank.

3. A crude protease enzyme extract collection system according to claim 1 wherein: still include the washing of being responsible for with the water source intercommunication, be equipped with tenth motorised valve and fourth pump body on the washing is responsible for, and it all communicates with four inner chambers and the collecting box of pre-buffer tank, circulation case through wasing the branch pipe, all is equipped with eleventh motorised valve on each wasing the branch pipe, and is located the container one end and all is equipped with the shower head.

4. A crude protease enzyme extract collection system according to claim 3 characterized in that: still including a plurality of washings casees that are used for storing different cleaners, each washs the case and all communicates with wasing the person in charge through annotating the liquid branch pipe, and annotates and be equipped with fifth pump body and first check valve on the liquid branch pipe in proper order by wasing the case to wasing the person in charge, first check valve is used for the separation washs to be responsible for to wasing the flow direction of case.

5. A crude protease enzyme extract collection system according to claim 3 characterized in that: the first liquid discharge pipe, the second liquid discharge pipe and the third liquid discharge pipe are all communicated to the cleaning main pipe, the communication position is located at one side of the water outlet end of the tenth electric valve, the first discharge branch pipe is communicated with the discharge end, and the twelfth electric valve is arranged on the first discharge branch pipe.

6. A crude protease enzyme extract collection system according to claim 3 characterized in that: the cleaning main pipe is communicated with the collecting area through an external cleaning branch pipe, a thirteenth electric valve is arranged on the external cleaning branch pipe, the collecting area is communicated with the discharging end through a second discharging branch pipe, and a fourteenth electric valve is arranged on the second discharging branch pipe.

7. A crude protease enzyme extract collection system according to claim 1 wherein: the first filter device is a first filter mesh bag sleeved on one end of the first collecting branch pipe communicated with the collecting box, and the second filter device is a second filter mesh bag sleeved on one end of the second collecting branch pipe communicated with the collecting box.

8. A method for collecting a crude protease extract, using the crude protease extract collecting system according to any one of claims 1 to 8, characterized in that: the method comprises the following steps:

9. The method for collecting a crude protease extract according to claim 8, characterized in that: when two proteases are collected by the two adsorption materials, the method further comprises the following steps: injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collection box through the first collection branch pipe, injecting the mixture of the urine and the corresponding adsorbing materials in the second inner cavity of the circulation box into the collection box through the second collection branch pipe, and pumping clear water to the corresponding spray heads through the cleaning main pipe and the cleaning branch pipes in the first inner cavity, the second inner cavity, the third inner cavity and the fourth inner cavity of the circulation box through the fourth pump body to spray out, and flushing the residual adsorbing materials on the screen plate;

10. The method for collecting a crude protease extract according to claim 8, characterized in that: the second check valve is arranged between the fourth electric valve and the spray header on the first circulation branch pipe and used for blocking the flow direction from the spray header to the fourth electric valve, the third check valve is arranged between the fifth electric valve and the spray header on the second circulation branch pipe and used for blocking the flow direction from the spray header to the fifth electric valve.