CN116036440A

CN116036440A - Visual stimulus experimental device

Info

Publication number: CN116036440A
Application number: CN202310078923.8A
Authority: CN
Inventors: 徐琳璐
Original assignee: Beijing Zhiyuan Artificial Intelligence Research Institute
Current assignee: Beijing Zhiyuan Artificial Intelligence Research Institute
Priority date: 2023-01-17
Filing date: 2023-01-17
Publication date: 2023-05-02
Anticipated expiration: 2043-01-17
Also published as: CN116036440B

Abstract

The application discloses visual stimulus experimental apparatus, including cylinder body and functional module subassembly be formed with the inner chamber that is used for placing the experiment body in the cylinder body, functional module subassembly includes a plurality of functional modules, each functional module be used for with the detachable connection of cylinder body. The visual stimulus experimental device provided by the application, because each functional module is used for being connected with the cylinder body in a detachable way, the functional module can be replaced when damaged, and the whole visual stimulus experimental device does not need to be manufactured again, so that the experimental cost is reduced, and the experimental efficiency is improved.

Description

Visual stimulus experimental device

Technical Field

The application relates to the technical field of visual stimulation, in particular to a visual stimulation experimental device.

Background

Visual stimulation is a common stimulation modality for conducting neuroscience-related experiments. At present, small animals such as fish are used as an experimental body, the device for carrying out the visual stimulation experiment is mainly customized for specific cases, and once functional components of the device for the visual stimulation experiment are damaged, the device for the whole visual stimulation experiment is usually required to be manufactured again.

Disclosure of Invention

In order to solve the problems in the prior art, the application provides the following technical scheme.

The application provides a visual stimulus experimental apparatus, including cylinder body and functional module subassembly be formed with the inner chamber that is used for placing the experiment body in the cylinder body, functional module subassembly includes a plurality of functional modules, each functional module be used for with the detachable connection of cylinder body.

Optionally, the functional module is used for being inserted with the cylinder body.

Optionally, the cylinder body includes enclosing into diapire and the lateral wall of inner chamber be formed with the grafting groove on the diapire, the notch of grafting groove is towards the inner chamber, the functional module with the grafting groove is pegged graft.

Optionally, at least two plugging grooves are formed on the bottom wall.

Optionally, the inner cavity is cube-shaped, the cylinder body has a jar mouth, the orientation of jar mouth is parallel to the thickness direction of inner cavity, the length direction of grafting groove is parallel to the length direction of inner cavity, each grafting groove is in the width direction of inner cavity is arranged.

Optionally, two ends of the plugging groove in the length direction of the inner cavity extend to two side walls of the cylinder body in a one-to-one correspondence manner.

Optionally, at least one the function module is a first experimental body fixing module, the cylinder body includes a bottom wall and a side wall both used for enclosing into the inner cavity, and the first experimental body fixing module is used for being detachably installed in the bottom wall.

Optionally, an experiment body accommodating groove is formed at one end of the first experiment body fixing module, one end, away from the experiment body accommodating groove, of the first experiment body fixing module is a matching end, and the matching end is used for being detachably connected with the bottom wall.

Optionally, the cylinder comprises a first plug connector, wherein a plug groove is formed in the bottom wall of the cylinder body, the first plug connector is used for being plugged with the plug groove, a clamping groove is formed in the first plug connector, and the matching end is used for being clamped with the clamping groove.

Optionally, the clamping groove is a trapezoid groove, and the sectional area of the clamping groove gradually decreases from the notch of the clamping groove to the bottom of the clamping groove.

Optionally, the first plug connector includes first grafting portion and first holding portion, the length direction of first grafting portion with the length direction of first holding portion is perpendicular, the both ends in the length direction of first grafting portion all are connected with first holding portion, so that first plug connector is the concave shape structure.

Optionally, the cylinder body has a jar mouth, first grafting portion be used for with the grafting groove is pegged graft, so that first holding portion is kept away from first grafting portion's one end stretches out the jar mouth.

Optionally, the first experimental body fixing module and the first plug connector are transparent structures.

Optionally, at least one of the functional modules is a second experimental body fixing module, and the second experimental body fixing module is detachably installed on the side wall.

Optionally, a mounting hole is formed on the side wall, the second experimental body fixing module includes a fixing tube, the experimental body is fixed in the fixing tube, and the fixing tube is spliced with the mounting hole.

Optionally, the mounting hole is the through-hole, the fixed module of second experiment body still includes the sealing plug, the sealing plug suit in fixed pipe, just the sealing plug is located outside the inner chamber in order to seal fixed pipe with clearance between the mounting hole.

Optionally, at least one of the functional modules is a visual stimulus screen module, and the visual stimulus screen module is detachably mounted on the bottom wall.

Optionally, the visual stimulation screen module comprises a screen perpendicular to the bottom wall, a plug groove is formed in the bottom wall of the cylinder body, and the second plug piece is used for being plugged with the plug groove so as to clamp the bottom end of the screen between the second plug piece and the side wall of the plug groove.

Optionally, the second plug connector includes second grafting portion and second holding portion, the length direction of second grafting portion with the length direction of second holding portion is perpendicular, both ends in the length direction of second grafting portion all are connected with the second holding portion, so that the second plug connector is concave shape structure.

Optionally, the cylinder body has a jar mouth, the second grafting portion is used for with the grafting groove grafting, so that the second holding portion is kept away from the one end of second grafting portion stretches out the jar mouth.

Optionally, a visual stimulus presentation window is provided on the side wall, the visual stimulus presentation window comprising a first transparent sheet, the screen being arranged parallel to the first transparent sheet.

Optionally, visual stimulus presentation windows are arranged on two side walls of the cylinder body which are oppositely arranged.

Optionally, the side wall includes first basis portion and first connecting portion, first basis portion with diapire integrated into one piece, first connecting portion detachable install in first basis portion, the border centre gripping of first transparent piece in first basis portion with between the first connecting portion.

Optionally, a behavior imaging window is disposed on the bottom wall, the behavior imaging window includes a second transparent sheet, an imaging window is formed on the bottom wall, the second transparent sheet covers the imaging window, the bottom wall includes a second base portion and a second connecting portion, the second base portion is integrally formed with the side wall, the second connecting portion is detachably connected with the second base portion, and the second transparent sheet is clamped between the second base portion and the second connecting portion.

Optionally, an inserting groove is formed on the bottom wall, the functional module is used for being inserted into the inserting groove, and the imaging window is formed at the bottom of the inserting groove.

The beneficial effects of this application are: the visual stimulus experimental device provided by the application, because each functional module is used for being connected with the cylinder body in a detachable way, the functional module can be replaced when damaged, and the whole visual stimulus experimental device does not need to be manufactured again, so that the experimental cost is reduced, and the experimental efficiency is improved.

Drawings

FIG. 1 is a schematic view of a partial perspective view of an embodiment of a visual stimulus experimental device provided in an example of the application;

FIG. 2 is a schematic partial perspective view of one embodiment of a cylinder provided in the examples of the present application;

FIG. 3 is a schematic view of a partially exploded construction of one embodiment of a cylinder provided in the examples of the present application;

FIG. 4 is a schematic partial perspective view of another embodiment of a cylinder provided in the examples of the present application;

FIG. 5 is a schematic view of a partially exploded construction of another embodiment of a cylinder provided in an example of the present application;

FIG. 6 is a schematic diagram of an exploded construction of one embodiment of a cylinder provided in an example of the present application;

FIG. 7 is a schematic view of a portion of another embodiment of a visual stimulus experimental device provided in an example of the application;

fig. 8 is a schematic structural diagram of a positional relationship between a first experimental body fixing module and a first connector according to an embodiment of the present application;

fig. 9 is a schematic structural diagram of a connection relationship between a visual stimulus screen module and a second connector according to an embodiment of the present disclosure;

fig. 10 is a schematic structural diagram of a positional relationship between a visual stimulus screen module and a second connector according to an embodiment of the present disclosure.

Wherein, 100, cylinder body; 110. a first base portion; 120. presenting a window; 130. a plug-in groove; 140. a mounting hole; 150. a second connecting portion; 140. a second base portion; 160. an imaging window; 170. a first connection portion; 180. an inner cavity; 200. a screen; 300. a second plug member; 310. a second holding part; 320. a second plug-in connection; 400. a first plug member; 410. a first grip portion; 420. a first plug-in connection; 430. a clamping groove; 500. the first experiment body fixing module; 510. an experiment body accommodating groove; 520. a mating end; 600. the second experiment body fixing module; 610. a sealing plug; 620. a fixed tube; 700. a first transparent sheet member; 800. a second transparent sheet.

Detailed Description

In order to better understand the above technical solutions, the following detailed description will be given with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1 to 10, an embodiment of the present application provides a visual stimulus experiment device, including a cylinder 100 and a functional module assembly, in which an inner cavity 180 for placing an experiment body is formed in the cylinder 100, and the functional module assembly includes a plurality of functional modules, and each of the functional modules is used for detachably connecting with the cylinder 100.

The experimental body in the embodiment of the application is fish, and the fish is preferably juvenile zebra fish; the functional module may be various kinds of functional components necessary for realizing the visual stimulus experiment, for example, may be a component for displaying a visual stimulus image, a component for fixing an experimental body, or the like. It will be appreciated that cylinder 100 is a container having a cylinder port at one end that communicates with interior cavity 180.

The visual stimulus experimental device provided by the application, because each functional module is used for being connected with the cylinder body 100 in a detachable way, the functional module can be replaced when damaged, and the whole visual stimulus experimental device does not need to be manufactured again, so that the experimental cost is reduced, and the experimental efficiency is improved.

Optionally, the functional module is configured to be plugged with the cylinder 100. The user can realize the dismouting of function module through the plug operation between function module and the cylinder body 100, easy dismounting. Of course, the functional module may be connected to the cylinder block 100 by a bolt connection.

Optionally, the cylinder 100 includes a bottom wall and a side wall enclosing the inner cavity 180, a plugging slot 130 is formed on the bottom wall, a notch of the plugging slot 130 faces the inner cavity 180, and the functional module is plugged with the plugging slot 130. Facilitating the installation of the functional module in the interior cavity 180. Of course, the plugging slot 130 may be disposed on the functional module, and a protrusion may be disposed on the bottom wall, so that the protrusion cooperates with the plugging slot 130 to realize detachable connection.

Optionally, at least two plugging grooves 130 are formed on the bottom wall. In this embodiment, the number of the plugging slots 130 is 2-5, and preferably, the number of the plugging slots 130 is 3. In this way, the user can select the appropriate socket 130 to connect with the corresponding functional module according to the experimental needs. In this embodiment of the present application, the relationship between the number of the jack slots 130 and the number of the functional modules may be that the number of the jack slots 130 is less than the number of the functional modules, the number of the jack slots 130 is equal to the number of the functional modules, or the number of the redundant functional modules of the jack slots 130, where each of the functional modules may have the same function, or at least two of the functional modules may have different functions. Therefore, the installation number and the types of the functional blocks can be flexibly selected according to experimental requirements, and the capability of the visual stimulus experimental device for adapting to different experimental modes is improved.

Optionally, the inner cavity 180 is cubic, the cylinder 100 has a cylinder opening, the cylinder opening is parallel to the thickness direction of the inner cavity 180, the length direction of the plugging slot 130 is parallel to the length direction of the inner cavity 180, and each plugging slot 130 is aligned in the width direction of the inner cavity 180.

Optionally, two ends of the plugging slot 130 in the length direction of the inner cavity 180 extend to two side walls of the cylinder 100 in a one-to-one correspondence.

Optionally, at least one of the functional modules is a first experimental body fixing module 500, the cylinder 100 includes a bottom wall and a side wall, which are both used for enclosing the inner cavity 180, and the first experimental body fixing module 500 is used for being detachably installed on the bottom wall.

Optionally, an experiment body accommodating groove 510 is formed at one end of the first experiment body fixing module 500, one end, far away from the experiment body accommodating groove 510, of the first experiment body fixing module 500 is a mating end 520, and the mating end 520 is used for being detachably connected with the bottom wall. In the embodiment of the present application, the test body may be immobilized in the test body accommodating groove 510 by a low melting point agarose gel. The head and tail of the juvenile fish can be fixed along the direction A-P in figure 8, so that the visual stimulus of left and right visual fields is realized. The L-R direction can be fixed, so that visual stimulus of front and back vision fields can be realized.

Optionally, the visual stimulus experimental apparatus provided by the embodiment of the application includes a first connector 400, a plugging slot 130 is formed on a bottom wall of the cylinder body 100, the first connector 400 is used for plugging with the plugging slot 130, a clamping slot 430 is formed on the first connector 400, and the mating end 520 is used for being clamped with the clamping slot 430. That is, the first experimental body fixing module 500 is indirectly fixed to the socket 130 through the first socket 400. The position of the clamping groove 430 on the first connector 400 can be designed according to the experiment requirement, so that the first experiment body fixing module 500 can select a proper fixing position on the length square of the inner cavity 180.

Alternatively, the clamping groove 430 is a trapezoid groove, and the cross-sectional area of the clamping groove 430 gradually decreases from the notch of the clamping groove 430 to the bottom of the clamping groove 430. When the matching end 520 moves gradually towards the clamping groove 430, the pressure between the matching end 520 and the inner wall of the clamping groove 430 can be gradually increased, so that the friction between the inner wall of the clamping groove 430 and the matching end 520 is increased, the matching end 520 can be firmly clamped in the clamping groove 430, meanwhile, the same first experimental body fixing module 500 can be tightly matched at different positions of the clamping groove 430, fine adjustment of the position of the first experimental body fixing module 500 is realized, and the stable and unchanged visual stimulation parameters among different experimental bodies when receiving stimulation are ensured, so that the stimulation parameters can be standardized. When the functional module assembly includes two or more differently sized first trial fixation modules 500, the trapezoidal shaped slot facilitates mating with differently sized mating ends 520. When the functional module assembly includes the first experimental body fixing module 500 of various specifications, it can be satisfied that the young zebra fish of a plurality of different ages of days is used as an experimental body to be fixed.

Optionally, the first connector 400 includes a first inserting portion 420 and a first holding portion 410, the length direction of the first inserting portion 420 is perpendicular to the length direction of the first holding portion 410, and two ends of the first inserting portion 420 in the length direction are connected with the first holding portion 410, so that the first connector 400 has a concave structure. Thus, when the first connector 400 is inserted into or removed from the socket 130, the user can hold the first holding portion 410 to operate, which is more convenient.

Optionally, the cylinder 100 has a cylinder opening, and the first plugging portion 420 is configured to plug into the plugging slot 130, so that an end of the first holding portion 410, which is far away from the first plugging portion 420, protrudes out of the cylinder opening. Thus, when the first plug 400 is inserted or pulled out, the user's hand can hold one end of the protruding cylinder port, so that the cylinder 100 is less likely to be a hindrance to the operation. In this embodiment, preferably, the top surface of the first plugging portion 420 is located in the plugging slot 130, or the top surface of the first plugging portion 420 is flush with the bottom wall.

Optionally, the first experimental body fixing module 500 and the first connector 400 are both transparent structures. Thus, the user's implementation is not easily blocked when observing the test body in the first test body fixing module 500.

Optionally, at least one of the functional modules is a second experimental body fixing module 600, and the second experimental body fixing module 600 is configured to be detachably mounted on the side wall. The visual stimulus experimental device comprises the first experimental body fixing module 500 and the second experimental body fixing module 600, and the first experimental body fixing module 500 or the second experimental body fixing module 600 can be selected according to experimental requirements.

Optionally, a mounting hole 140 is formed on the side wall, the second experimental body fixing module 600 includes a fixing tube 620, the experimental body is fixed in the fixing tube 620, and the fixing tube 620 is inserted into the mounting hole 140.

Optionally, the mounting hole 140 is a through hole, the second experimental body fixing module 600 further includes a sealing plug 610, the sealing plug 610 is sleeved on the fixing tube 620, and the sealing plug 610 is located outside the inner cavity 180 to seal a gap between the fixing tube 620 and the mounting hole 140. The mounting hole 140 is a through hole, and the fixing tube 620 can be inserted into the inner cavity 180 from outside the inner cavity 180, so that the fixing tube 620 is convenient to mount, and the gap between the fixing tube 620 and the mounting hole 140 is sealed by the sealing plug 610, so that the liquid in the cylinder 100 is not easy to leak out through the mounting hole 140. Of course, the mounting hole 140 may be a blind hole. In this embodiment, preferably, when the second experimental body fixing module 600 is not used and the first experimental body fixing module 500 is used, the sealing plug 610 is also fixed at the mounting hole 140, and when the fixing tube 620 is needed, only the sealing plug 610 and the mounting hole 140 need to be sequentially penetrated. The fixing tube 620 is preferably a glass tube.

Optionally, at least one of the functional modules is a visual stimulus screen module, and the visual stimulus screen module is detachably mounted on the bottom wall. The visual stimulus experimental device provided by the embodiment of the application can realize the switching of different use modes of the visual stimulus experimental device when the visual stimulus experimental device comprises a modularized visual stimulus screen module, a first experimental body fixing module 500 and a second experimental body fixing module 600, and each module can be independently replaced and modified, so that the maintenance cost is reduced.

Optionally, the visual stimulus experimental apparatus provided by the embodiment of the application includes a second connector 300, the visual stimulus screen module includes a screen 200 perpendicular to the bottom wall, a socket 130 is formed on the bottom wall of the cylinder 100, and the second connector 300 is used for being connected with the socket 130 in a socket manner, so that the bottom end of the screen 200 is clamped between the second connector 300 and the side wall of the socket 130.

Optionally, the second connector 300 includes a second connector portion 320 and a second holding portion 310, the length direction of the second connector portion 320 is perpendicular to the length direction of the second holding portion 310, and two ends of the second connector portion 320 in the length direction are connected with the second holding portion 310, so that the second connector 300 has a concave structure. In this way, when the second connector 300 is inserted into or removed from the insertion groove 130, the user can hold the second holding portion 310 to operate, which is more convenient.

Optionally, the cylinder 100 has a cylinder opening, and the second plugging portion 320 is configured to plug into the plugging slot 130, so that an end of the second holding portion 310, which is far away from the second plugging portion 320, protrudes out of the cylinder opening. Thus, when the second plug 300 is inserted or pulled out, the user's hand can hold one end of the protruding cylinder port, so that the cylinder 100 is less likely to be a hindrance to the operation. In this embodiment, preferably, the top surface of the second plugging portion 320 is located in the plugging slot 130, or the top surface of the second plugging portion 320 is flush with the bottom wall.

Optionally, a visual stimulus presentation window is provided on the side wall, the visual stimulus presentation window comprising a first transparent sheet 700, the screen 200 being arranged parallel to the first transparent sheet 700. It will be appreciated that the user may view the conditions in the interior cavity 180 from outside the cylinder 100 through the visual stimulus presentation window, through which the subject may view the conditions outside the cylinder 100; a presentation window 120 is first opened in the sidewall, and then a first transparent sheet 700 is installed in the presentation window 120. Thus, when the experiment needs to obtain visual stimulus through the visual stimulus presentation window, the visual stimulus screen module can be detached from the cylinder 100, and when the experiment needs to present visual stimulus through the screen 200, the visual stimulus screen module can be mounted on the cylinder 100.

Optionally, visual stimulus presentation windows are provided on both side walls of the cylinder 100, which are disposed opposite to each other.

Optionally, the side wall includes a first base portion 110 and a first connecting portion 170, where the first base portion 110 and the bottom wall are integrally formed, the first connecting portion 170 is detachably mounted on the first base portion 110, and an edge of the first transparent sheet 700 is clamped between the first base portion 110 and the first connecting portion 170. In this way, the first transparent sheet 700 is easy to disassemble and assemble, and the first transparent sheet 700 is easy to replace and clean. Preferably, the first base part 110 and the first connection part 170 may be connected by a screw; the first connection 170 is located outside the interior cavity 180; the first transparent sheet 700 may be adhered between the first base part 110 and the first connection part 170 with waterproof glue; the first transparent sheet 700 is preferably a glass sheet.

Optionally, a behavioral imaging window is disposed on the bottom wall, the behavioral imaging window includes a second transparent sheet 800, an imaging window 160 is formed on the bottom wall, the second transparent sheet 800 covers the imaging window 160, the bottom wall includes a second base portion 140 and a second connecting portion 150, the second base portion 140 is integrally formed with the side wall, the second connecting portion 150 is detachably connected with the second base portion 140, and the second transparent sheet 800 is clamped between the second base portion 140 and the second connecting portion 150. Thus, the user can acquire the behavior imaging of the experimental body in the inner cavity 180 through the behavior imaging window, so that the second transparent piece can be conveniently disassembled and assembled, and the second transparent piece can be conveniently cleaned and more slide. Preferably, the second base part 140 and the second connection part 150 may be connected by a screw; the second connection 150 is located outside the lumen 180; the second transparent sheet 800 may be adhered between the second base part 140 and the second connection part 150 with waterproof glue; the second transparent sheet 800 is preferably a glass sheet.

Optionally, a plugging slot 130 is formed on the bottom wall, the functional module is used for plugging with the plugging slot 130, and the imaging window 160 is formed at the bottom of the plugging slot 130.

In order to better illustrate the visual stimulus experimental device provided by the application, the application also provides an application example of the visual stimulus experimental device.

Visual stimulation is a common stimulation modality for neuroscience-related experiments using zebra fish. The prior devices for carrying out the experiments are mainly customized for specific cases, and have the defects of weak universality, difficult stimulation pattern conversion, partial damage, need to be manufactured again and the like. And the stimulation parameters are difficult to normalize due to different manufacturing quality.

The technical scheme provided by the application example has the core conception that: (1) The application satisfies the visual stimulus presentation mode in most zebra fish experiments at present and the fixed mode of zebra fish juvenile fish through designing specific chamber structure to this promotes the application under multiple experimental demand's suitability, reaches the demand that an experimental apparatus can carry out multiple experiment. (2) In addition, the switch of different using modes is realized by modularization of the experimental device, and each module can be independently replaced and modified, so that the maintenance cost is reduced. (3) The design scheme fully considers the stability and repeatability of the visual stimulus parameter presentation. Through designing specific plug-in components and promoting the mode that sample position can be finely adjusted, guarantee that the parameter of visual stimulus is stable unchanged among the samples when the sample is receiving the stimulus, make the stimulus parameter can realize standardization.

Specific embodiments of the present application example: (1) The multi-view stimulation presentation design meets the presentation requirements of various experimental stimulation, namely the closed water environment with a chamber structure, and meets the requirements of the juvenile fish immersed in the water. At present, two stimulus presentation windows are arranged on the chamber, so that the requirements of presenting visual stimulus at the left side and the right side simultaneously or presenting stimulus at the front and the rear visual fields of young fish simultaneously can be met. (2) The modularized plug-in unit meets the requirements of various experimental designs, namely a module plug-in slot is constructed in the chamber, so that the requirement of device modularization is further met. Different plug-ins can be installed to meet different experimental design requirements. Such as a "visual stimulus screen insert" is used to present visual stimulus. The fixing plug-in is used for stabilizing the fine adjustment and repeatability of the position of the juvenile fish fixing module. Meanwhile, the design of the plug-in slot also meets the stability of related stimulation parameters. (3) The two juvenile fish fixing schemes meet the fixing requirements of different samples-the application designs the two juvenile fish fixing schemes. According to the scheme I, the square transparent grooves are used for fixing the juvenile fish, so that the stability of the juvenile fish in gel is improved, and the related imaging effect is stable. And can meet the semi-fixed requirement of the juvenile fish, and output some behaviors such as tail movement or eye movement in experiments. In the experiment, this module can take out alone, has promoted the convenience of use. And the parameters presented to the sample by the stimulus can be fine-tuned by adjusting the module parameters. The second scheme is to fix the juvenile fish with a transparent glass tube. The scheme enables the juvenile fish to have a better field of view (because there is no occlusion in front of the eyes) and the observed angle of the sample can be finely adjusted. (4) The design of the chamber bottom observation window meets the requirement of behavioral monitoring, namely in some zebra fish nerve experiments, researchers need to observe the behavior of young fish when receiving stimulus, and then make corresponding analysis. The observation window designed at the bottom of the Chamber meets the requirement of real-time behavior monitoring of the juvenile fish in the experiment, and the behavior of the juvenile fish can be collected while the related neuroimaging/neural signal recording is carried out.

The device mainly comprises the following parts:

(1) The chamber, the five sealed faces are open and form a jar-shaped structure capable of containing water, and the jar-shaped structure is used for providing water environment meeting the living demands of zebra fish. Two visual stimulus presentation windows, a group of behavior imaging acquisition windows and four interfaces for inserting experimental modules are arranged on the device.

The main body of the Chamber is a framework made of metal materials, so that the abrasion resistance of the Chamber is effectively improved. The bottom of the inner part is provided with three grooves with the same width (a groove I, a groove II and a groove III) for inserting other modules required by experiments, such as a mini projection screen and a juvenile fish fixing module 1. The modules can be stably inserted into any one of the grooves through the brackets matched with the grooves in size, so that stable spatial relationship is formed among the modules. Taking the first drawing as an example, the first juvenile fish fixing module is inserted in the first groove, the mini projection screen is inserted in the third groove, and visual stimulus which is originally from a right side view field or a front-back view field can be provided for the zebra fish. In order to meet different experimental requirements, the modules inserted into the grooves can be assembled by themselves according to experimental design. The groove can also be used as a support, a new module required by experiments is designed by self, and the module is inserted into the groove to complete assembly. The design improves the flexibility and convenience of the vision experimental device. As shown in fig. 2, the lower parts of the three grooves can be made into transparent bottoms (shown in fig. 4) and non-transparent bottoms (shown in fig. 2) according to experimental requirements so as to meet different behavioural imaging data acquisition requirements.

The bottom of the main body of the Chamber is provided with a behavior imaging window, a glass sheet is embedded in the window, waterproof glue is used for realizing sealing, and the glass sheet is fixed at the bottom of the Chamber main body through a bottom window cover (four corners of the bottom window cover are hollow and are fixed through screws). The bottom window cover increases the tightness of the behavioural imaging window and protects the glass sheet of the behavioural imaging window. The behavioural imaging window may be selected to be of an open window size (as shown in figures 3 and 5) depending on the experimental requirements. Real-time recording of zebra fish behavior can be achieved by assuming a behavior camera under a behavior imaging window.

The left side and the right side of the Chamber are respectively a visual stimulus presentation window I and a visual stimulus presentation window II, and visual stimulus is transmitted into the eyes of the zebra fish through the two windows. The first visual stimulus presentation window and the second visual stimulus presentation window each comprise a sheet of glass and a visual stimulus window cover. The glass sheet is glued to the window by means of a waterproof glue, and then a visual stimulus window cover is placed over the glass sheet, which is secured to the chamber body by means of screws. The visual window cover can increase the tightness of the chamber and improve the protection of the glass sheet.

(2) Module I-Mini projection Screen, presents the visual stimulus projected by the projector. Visual experimental stimuli to be presented to the juvenile fish in the experiment can be projected onto a mini projection screen through a projector and then presented to the juvenile fish.

The mini projection screen comprises a projection screen bracket and a mini projection screen. A mini-projection screen is required when visual stimuli need to be presented to the zebra fish by a projector. The projection screen bracket is perfectly matched with the first groove, the second groove or the third groove in the chamber, and can be arranged in any groove. The mini projection screen is glued to the projection screen holder using glue.

(3) The first juvenile fish fixing module is a first fixing mode, and the mode of fixing juvenile fish by using square grooves is met.

The first juvenile fish fixing module comprises a juvenile fish fixing bracket and a juvenile fish fixing block, and the juvenile fish fixing bracket and the juvenile fish fixing block are made of transparent materials so as to facilitate behavior imaging. The juvenile fish fixing support is provided with a groove matched with the juvenile fish fixing block, and the juvenile fish fixing block is arranged on the fixing frame through being clamped in the groove. When the zebra fish fixing device is used, the zebra fish fixing block is only required to be taken down to fix the zebra fish, and the zebra fish is convenient to take. The juvenile fish fixing bracket does not need to be taken out when the juvenile fish is fixed. The groove opening of the juvenile fish fixing support is of a trapezoid design, so that stability of the juvenile fish fixing block after being installed in can be reinforced. The position of the groove of the juvenile fish fixing support on the support can be adjusted according to experimental requirements, and the support meeting experimental parameters is designed and manufactured, so that the relative spatial position of the juvenile fish and visual stimulus in the horizontal direction is adjusted. The juvenile fish fixing block is a transparent cube, a square groove is dug at the top, and juvenile fish can be fixed in the groove through low-melting-point agarose gel. The head and tail of the juvenile fish can be fixed along the direction A-P in figure 8, so that the visual stimulus of left and right visual fields is realized. The L-R direction can be fixed, so that visual stimulus of front and back vision fields can be realized. The adjustment of the relative spatial positions of the juvenile fish and the visual stimulus in the vertical direction can be realized by manufacturing juvenile fish fixing blocks with different heights.

(4) And the third module is a juvenile fish fixing module II, and the juvenile fish fixing module II is in a second fixing mode, so that the mode of fixing juvenile fish by using a glass round tube is satisfied. As shown in fig. 7.

The juvenile fish fixing module II comprises a fixing hole, a rubber plug and a fixing tube. The fixing hole penetrates through the main body of the chamber, and the fixing tube can penetrate through the fixing hole and extend into the chamber (as shown in fig. 8). The center of the rubber plug is provided with a hole which is equal to the fixed pipe in size, the rubber plug is concentrically arranged with the fixed hole, and the rubber plug is adhered to the chamber main body through glue. The plug can prevent that the water in the chamber from oozing in the fixed orifices to can increase the stability that the fixed pipe was placed, and through increasing the frictional resistance of fixed pipe when removing, reach the demand of fine adjustment zebra fish position. Zebra fish is fixed in a fixed tube through agarose gel with low melting point, and is sent to the inside of a chamber through a hole in the center of a rubber plug and a fixed hole. The relative position of the juvenile fish in the chamber can be adjusted through the push-pull fixing pipe, and the juvenile fish can be adjusted to be placed through the rotary fixing pipe.

The application example has the beneficial effects that:

(1) Is suitable for various zebra fish visual stimulus experiments, designs windows with different angles for presenting visual stimulus, and designs two young fish fixing modules

(2) The use mode is diversified, the maintenance is simple, the modularized design is convenient for the arbitrary assembly and the independent replacement of two parts

(3) The juvenile fish fixation position is fine-tuned—the insert design improves the stability of sample placement, fine-tuning, and repeatability across samples.

Specifically:

1. and (3) modular design: the module needed by the visual experiment is not fixed in the chamber, but is formed by combining and inserting module inserts which are matched with grooves in the chamber as required. Therefore, according to different experiments, the placement of each module can be flexibly implemented, and new plug-ins can be designed to realize different requirements. And for the same experiment, one plug-in is damaged without the need of remanufacturing the whole device.

2. The method is suitable for various zebra fish visual stimulus experiments: through designing three windows, the method can meet the requirement of acquiring behavior data while the visual stimulus is input through multiple angles of the windows, so that the presentation modes of different visual stimuli are realized. Different module plug-ins are combined to customize and meet different experimental requirements.

3. Fine adjustment of the field of view space of young fish: the fine adjustment of the fixing position of the juvenile fish is realized by purposefully manufacturing module plug-ins with different parameters (such as the opening positions of grooves in the juvenile fish fixing support of the juvenile fish fixing module I, and pushing and pulling the juvenile fish fixing pipe.

While preferred embodiments of the present application have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the application. It will be apparent to those skilled in the art that various modifications and variations can be made in the present application without departing from the spirit or scope of the application. Thus, if such modifications and variations of the present application fall within the scope of the claims and the equivalents thereof, the present application is intended to cover such modifications and variations.

Claims

1. The visual stimulus experiment device is characterized by comprising a cylinder body and a functional module assembly, wherein an inner cavity for placing an experiment body is formed in the cylinder body, the functional module assembly comprises a plurality of functional modules, and each functional module is detachably connected with the cylinder body.

2. The visual stimulus testing device of claim 1, wherein the functional module is configured to interface with the cylinder.

3. The visual stimulus testing device of claim 2, wherein said cylinder body comprises a bottom wall and a side wall enclosing said cavity, a mating groove is formed in said bottom wall, a notch of said mating groove faces said cavity, and said functional module mates with said mating groove.

4. A visual stimulus testing device according to claim 3, characterized in that at least two of said insertion grooves are formed in said bottom wall.

5. The visual stimulus testing device of claim 4, wherein said interior cavity is cubical in shape, said cylinder having a cylinder opening oriented parallel to a thickness direction of said interior cavity, a length direction of said mating grooves being parallel to a length direction of said interior cavity, each of said mating grooves being aligned in a width direction of said interior cavity.

6. The visual stimulus testing device of claim 5, wherein two ends of the insertion groove in the length direction of the inner cavity extend to two side walls of the cylinder body in one-to-one correspondence.

7. The visual stimulus testing device of claim 1, wherein at least one of said functional modules is a first test body securing module, said cylinder including a bottom wall and a side wall each adapted to enclose said interior cavity, said first test body securing module adapted to be removably mounted to said bottom wall.

8. The visual stimulus testing device of claim 7, wherein a test body receiving slot is formed at one end of the first test body fixing module, and wherein the end of the first test body fixing module remote from the test body receiving slot is a mating end for detachable connection with the bottom wall.

9. The visual stimulus testing device of claim 8, comprising a first plug-in connector having a plug-in slot formed in a bottom wall of the cylinder, the first plug-in connector adapted to plug into the plug-in slot, a slot formed in the first plug-in connector, and the mating end adapted to be snapped into the slot.

10. The visual stimulus testing device of claim 9, wherein said clamping groove is a trapezoidal groove, and wherein a cross-sectional area of said clamping groove gradually decreases from a notch of said clamping groove to a groove bottom of said clamping groove.

11. The visual stimulus experiment apparatus according to claim 9, wherein the first plug-in unit includes a first plug-in portion and a first holding portion, a longitudinal direction of the first plug-in portion is perpendicular to a longitudinal direction of the first holding portion, and both ends in the longitudinal direction of the first plug-in portion are connected with the first holding portion, so that the first plug-in unit is of a concave structure.

12. The visual stimulus testing device of claim 11, wherein the cylinder has a cylinder opening, and the first mating portion is configured to mate with the mating groove such that an end of the first holding portion remote from the first mating portion extends out of the cylinder opening.

13. The visual stimulus testing device of claim 9, wherein the first test body fixation module and the first plug-in connector are both transparent structures.

14. The visual stimulus testing device of claim 7, wherein at least one of said functional modules is a second test body securing module for detachable mounting to said side wall.

15. The visual stimulus testing device of claim 14, wherein a mounting hole is formed in the side wall, the second test body securing module comprising a securing tube, the test body being adapted to be secured within the securing tube, the securing tube being mated with the mounting hole.

16. The visual stimulus testing device of claim 15, wherein the mounting hole is a through hole, the second test body securing module further comprises a sealing plug that fits around the securing tube, and the sealing plug is positioned outside the interior cavity to seal a gap between the securing tube and the mounting hole.

17. The visual stimulus testing device of any one of claims 7-16, wherein at least one of said functional modules is a visual stimulus screen module for detachable mounting to said bottom wall.

18. The visual stimulus experiment apparatus of claim 17, comprising a second connector, wherein the visual stimulus screen module comprises a screen for being disposed perpendicularly to the bottom wall, a connector slot being formed on the bottom wall of the cylinder, the second connector being for being connected to the connector slot to clamp the bottom end of the screen between the second connector and the side wall of the connector slot.

19. The visual stimulus testing device of claim 18, wherein the second plug comprises a second plug portion and a second holding portion, the length direction of the second plug portion is perpendicular to the length direction of the second holding portion, and the second holding portion is connected to both ends of the second plug portion in the length direction, so that the second plug is in a concave structure.

20. The visual stimulus testing device of claim 19, wherein said cylinder has a cylinder opening, and said second mating portion is adapted to mate with said mating groove such that an end of said second holding portion remote from said second mating portion extends out of said cylinder opening.

21. Visual stimulus testing device according to claim 17, characterized in that a visual stimulus presentation window is provided on the side wall, the visual stimulus presentation window comprising a first transparent sheet, the screen being arranged parallel to the first transparent sheet.

22. The visual stimulus testing device of claim 21, wherein visual stimulus presentation windows are provided on each of the oppositely disposed side walls of the cylinder.

23. The visual stimulus testing device of claim 21, wherein the side wall comprises a first base portion and a first connecting portion, the first base portion being integrally formed with the bottom wall, the first connecting portion being removably mounted to the first base portion, the edge of the first transparent sheet being clamped between the first base portion and the first connecting portion.

24. Visual stimulus testing device according to any of the claims 7-16, characterized in that a behavioural imaging window is provided on the bottom wall, the behavioural imaging window comprising a second transparent sheet, an imaging window being formed on the bottom wall, the second transparent sheet covering the imaging window, the bottom wall comprising a second base part and a second connecting part, the second base part being integrally formed with the side wall, the second connecting part being detachably connected with the second base part, the second transparent sheet being clamped between the second base part and the second connecting part.

25. The visual stimulus testing device of claim 24, wherein a mating groove is formed in said bottom wall, said functional module being adapted to mate with said mating groove, said imaging window being formed in a bottom of said mating groove.