CN113940306A - Novel vessel for zebra fish behaviouristics experiments - Google Patents
Novel vessel for zebra fish behaviouristics experiments Download PDFInfo
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- CN113940306A CN113940306A CN202111326490.0A CN202111326490A CN113940306A CN 113940306 A CN113940306 A CN 113940306A CN 202111326490 A CN202111326490 A CN 202111326490A CN 113940306 A CN113940306 A CN 113940306A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 74
- 241000252212 Danio rerio Species 0.000 title claims abstract description 47
- 125000006850 spacer group Chemical group 0.000 claims abstract description 45
- 230000003542 behavioural effect Effects 0.000 claims abstract description 37
- 238000005192 partition Methods 0.000 claims description 15
- 238000009434 installation Methods 0.000 claims description 11
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- 238000000638 solvent extraction Methods 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 5
- 108700024526 zebrafish sox32 Proteins 0.000 abstract description 2
- 241000251468 Actinopterygii Species 0.000 description 13
- 241001465754 Metazoa Species 0.000 description 8
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a novel zebra fish vessel for behavioural experiments, which comprises a cavity body, wherein the cavity body comprises an experiment target observation area and an experiment environment configuration area; the experimental target observation area is arranged in the center of the cavity body, and the experimental environment configuration area surrounds the experimental target observation area; the experimental environment configuration area comprises a baffle plate and a spacer; the baffle longitudinally separates the experiment environment configuration area, and the spacer is detachably connected with the baffle; the spacer carries information for stimulation, and has the advantages that the experimental environment is flexible and changeable, the experimental requirements for the specialization of zebra fish can be met, the supplementary test means is facilitated, and the test range is enlarged.
Description
Technical Field
The invention relates to the technical field of experimental equipment, in particular to a novel zebra fish behavioral experiment vessel.
Background
With the development of model animal zebra fish experiments and the attention of people to brain science, the attention degree of learning memory and complex behaviors of organisms is gradually increased in the world, the existing zebra fish behavioral related experiments are mainly based on a T maze and a cross maze, and the vessels can be customized according to different experimental purposes so as to meet the experimental requirements including color tendency, reward and punishment memory and the like, but are concentrated on adult zebra fish, the attention to juvenile fish is insufficient, and the early life is of great importance as an important window period for intelligence development and environmental exposure.
The currently used behavioural maze has a single exposure factor, needs to be repeatedly customized according to the experimental purpose, and simultaneously defines a certain 'non-exposure' area, and actually the animal in the area is also influenced by the exposure factor, and has brain activity and choice, however, how to define the range is difficult, and simultaneously, the complex environmental exposure is lacked, and the research on the complex exposure and the complex movement of the animal environment is limited.
Chinese patent document CN110558247A discloses a combined multi-arm labyrinth device, which includes a separation arm and combined steps, the separation arm is a one-way passage structure composed of two side plates and a sealing plate, one end of the two side plates is sealed by the sealing plate, the other end is open, the separation arm is provided with a plurality of separation arms, the open ends of the plurality of separation arms are sequentially connected in the circumferential direction, and a central dead zone is enclosed in the middle, and a combined step is provided in the one-way passage of each separation arm; the method can be used for experimental study of animal motion behavior control and experimental study of animal learning and memory ability; the technical scheme provided by the patent document provides different movement direction selection obstacles through the assembly of a plurality of separating arms along different directions and the establishment of combined steps therein, and sets space obstacles on the road to carry out experimental research on land and aquatic animals, obviously aiming at behavioural experiments, wherein a baffle can be temporarily placed between a central dead zone and each separating arm to realize the temporary closing and opening of a channel, and the aim is to train the memory capacity of a rat; the zebra fish behavioural experimental device to be solved by the application can not be used universally and flexibly, and can not be solved, for example, the shape of the central dead zone is determined by the number of the separating arms, and in the experiment of visual-kinetic reaction, the experiment of the movement effect of the grating can not be realized.
Chinese patent document CN213848209U discloses a composite animal ethology maze device, which comprises a circular bottom plate, wherein the top of the circular bottom plate is provided with an annular side wall, the annular side wall is arranged at the edge of the circular bottom plate by a circle, the circular bottom plate is provided with a track capable of forming a T-maze, a Y-maze, an eight-arm maze and an elevated cross maze, the border of the track and the annular side wall is provided with a hole, and a side arm plate can be inserted into the hole. The device can realize that four animal behavioristics mazes are contained in one mazy main body, the detachable side arm plate realizes the conversion between different mazes, and the required animal behavioristics mazes are assembled by inserting the side arm plate into different holes according to the track. The device can meet the requirements of four experiments of a T maze, a Y maze, an eight-arm maze and an elevated cross maze, has the characteristic of multiple functions, greatly simplifies the complexity of replacing the device among different experiments, and saves the space and time. In the same way, the technical scheme integrates the four labyrinths into one device, does not form breakthrough to the experimental steps, and only can be assembled by using fewer experimental devices compared with the traditional experiment.
In conclusion, a novel zebra fish behavior experiment vessel which is flexible and changeable in experiment environment, can meet experiment requirements for zebra fish specialization, is beneficial to supplement of experiment means and expansion of experiment range is needed.
Disclosure of Invention
The invention aims to provide a novel experimental vessel for zebra fish behavioural experiments, which has flexible and changeable experimental environment, can meet the experimental requirements aiming at the specialization of zebra fish, is beneficial to supplementing the experimental means and expanding the experimental range.
In order to achieve the purpose, the invention adopts the technical scheme that:
a novel zebra fish vessel for behavioural experiments comprises a cavity body, wherein the cavity body comprises an experiment target observation area and an experiment environment configuration area; the experimental target observation area and the experimental environment configuration area are separated by a transparent partition plate, the experimental target observation area is arranged in the center of the cavity body, and the experimental environment configuration area surrounds the experimental target observation area; the experimental environment configuration area comprises a baffle plate and a spacer; the baffle is used for partitioning the experiment environment configuration area, and the spacer is detachably connected with the baffle and used for changing the area environment.
As a preferable technical solution, the baffle is perpendicular to the side walls of the experiment target observation area and the experiment environment configuration area.
As a preferable technical solution, the baffle plate divides the experiment environment configuration area into four equal parts.
As a preferred technical scheme, a plurality of first connecting parts are arranged on the baffle plate, and the first connecting parts are detachably connected with the spacer.
As a preferred technical scheme, the first connecting portion is of a slot structure, and the spacer is detachably connected with the adjacent baffle.
As a preferable technical solution, the first connecting portion is a slot penetrating through the baffle, and the spacer is movable along the slot.
As a preferred technical scheme, the bottom of a central area of the cavity body in the experimental target observation area is also provided with an installation part, and the installation part is provided with a stop post detachably connected with the installation part.
As a preferable technical solution, the mounting portion and the stopper are provided in plurality and have different sizes.
As a preferred technical scheme, the partition plate is detachably connected with the cavity body.
As a preferred technical solution, it is characterized in that the spacer carries information thereon, which may be a grating, a color stripe or an integrated LED display, and the spacer may also be a display screen.
The invention has the advantages that:
1. the novel zebra fish behavioral experiment vessel comprises a cavity body, wherein the cavity body comprises an experiment target observation area and an experiment environment configuration area; the experimental target observation area can be used for placing target fish species, so that the behavior of the target fish can be observed and recorded conveniently, the experimental environment configuration area can flexibly configure stimulation environment according to experimental needs, and the research of univariate and/or multivariate can be flexibly realized.
2. The experimental target observation area and the experimental environment configuration area are separated by a transparent partition plate, so that the receiving of the stimulation by the experimental object is not influenced.
3. The experimental environment configuration area comprises a baffle plate and a spacer; the baffle is used for carrying out the subregion to experimental environment configuration district, and what correspond, its subregion has fixed angle, therefore its subregion can extend to experimental target observation district does benefit to the judgement to target fish activity, the spacer with the baffle can be dismantled and link to each other, can change the regional environment that is separated by the baffle in a flexible way to provide different stimulations.
4. The baffle is perpendicular to the side walls of the experiment target observation area and the experiment environment configuration area, and partition division, namely confirmation of an affected area, is facilitated.
5. The experimental environment configuration area is divided into four equal parts by the baffle, the method belongs to a conventional behavior area division method, and is simple and clear and high in reliability.
6. The baffle is equipped with a plurality of first connecting portion on it, first connecting portion can be dismantled with the spacer and be connected, first connecting portion can be slot structure, has both made things convenient for the dismantlement of spacer, can provide amazing far and near test contrast through the spacer of loading and unloading different positions again.
7. The first connecting part is a clamping groove penetrating through the baffle plate, so that the spacer can move between different baffle plates along the clamping groove, and experimental conditions for moving targets are provided.
8. The cavity body is located the central zone bottom in experiment target observation district and still is equipped with the installation department, be equipped with on it rather than dismantling the shelves post that links to each other, the installation department with the fender post is equipped with a plurality of, and has different size of a dimension, the shelves post can block experiment target observation district to the completion is to "exposing the district" and the verification test of "non-exposure district".
Drawings
FIG. 1 is a three-dimensional schematic view of a novel zebra fish behavioral experiment vessel.
FIG. 2 is a schematic diagram of the spacer and the first connecting part of the novel zebra fish behavioral experiment vessel.
Fig. 3 is a top view of another novel zebra fish behavioral experiment vessel of the invention.
FIG. 4 is a schematic perspective view of another novel zebra fish behavioral experiment vessel of the invention.
FIG. 5 is a schematic diagram of another novel zebra fish behavioral experiment vessel of the invention.
FIG. 6 is a schematic diagram of the matching of a vessel and a bumping post for a novel zebra fish behavioural experiment of the invention.
FIG. 7 is a schematic diagram of a cross-like maze form of the novel zebra fish vessel for behavioural experiments.
Detailed Description
The invention will be further illustrated with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the disclosure of the present invention, and equivalents fall within the scope of the appended claims.
The reference numerals and components referred to in the drawings are as follows:
| 1. |
11. Experimental |
12. Experimental |
| 13. |
14. Mounting |
15. Bumping |
| 121. Baffle |
122. |
1211. First connecting part |
Referring to the attached drawings 1 and 2, fig. 1 is a three-dimensional schematic view of a novel zebra fish behavioral experimental vessel; FIG. 2 is a schematic diagram of the spacer and the first connecting part of the novel zebra fish behavioral experiment vessel. A novel zebra fish vessel for behavioural experiments comprises a cavity body 1, wherein the cavity body 1 comprises an experiment target observation area 11 and an experiment environment configuration area 12; the experimental target observation area 11 is arranged in the center of the cavity body 1, and the experimental environment configuration area 12 surrounds the experimental target observation area 11; the experimental environment configuration area 12 comprises a baffle 121 and a spacer 122; the baffle 121 longitudinally separates the experimental environment configuration area 12, and the spacer 122 is detachably connected with the baffle 121;
it should be noted that: the cavity body 1, namely a water storage tank, is internally divided into two areas by outward diffusion from the center, an experimental target observation area 11 is positioned at the center, and an experimental environment configuration area 12 is positioned outside the experimental target observation area 11; the experimental target observation area 11 and the experimental environment configuration area 12 are isolated from each other, that is, the target fish thrown into the experimental target observation area 11 is limited to the activity in the area; the experimental environment configuration area 12 includes a baffle 121 and a spacer 122, the baffle 121 provides support for the spacer 122, the baffle 121 and the spacer 122 divide the experimental environment configuration area 12 into several areas, for example, the baffle 121 may divide the experimental environment configuration area 12 longitudinally in a cross shape, which is equivalent to divide it into four quadrants, the quadrants are divided and extend to the center of the experimental target observation area 11, experimental environments of different quadrants may be changed during the experiment, and whether the gathering condition of target fish in the experimental target observation area 11 tends to concentrate in a certain quadrant is observed to record the experimental result under the change of the relevant experimental environment, therefore, it is obvious that the spacer 122 is detachably connected to the baffle 121, and the spacer 122 is one of the factors of the change of the experimental environment, such as color, brightness, etc. of the spacer 122, and the distance relationship of the setting position of the spacer 122 with specific characteristics, etc., it is the carrier of experimental elements.
The novel zebra fish behavioral experiment vessel can change experiment environments at any time, flexibly carry out experiments, break the limitation of the existing experiment steps, and conveniently arrange the combined stimulation action of various conditions to carry out experiments.
Example 1
Please refer to fig. 1 and fig. 2 again; a novel zebra fish behavioral experiment vessel comprises a cavity body 1, wherein the cavity body 1 is a cylindrical cavity, the cavity is internally provided with a partition plate 13 with concentric circles, the partition plate 13 is made of transparent material and divides the cavity into two areas, the central area is an experimental target observation area 11, the outer side is an experimental environment configuration area 12, a baffle 121 is arranged between the experiment target observation area 11 and the experiment environment configuration area 12, the baffle 121 is perpendicular to the side walls of the two zones, and divides the experimental environment configuration zone 12 into four equal parts, namely, the baffle 121 is divided into a first quadrant region, a second quadrant region, a third quadrant region and a fourth quadrant region, wherein a plurality of first connecting portions 1211 are disposed on the baffle 121, in this embodiment, the first connecting portions 1211 are detachably connected to the spacer 122, and are in a slot structure, correspondingly, in this embodiment, the spacers 122 are concentric arc pieces and can be transversely inserted into the slots of the adjacent baffles 121; the spacer 122 is an information carrier, for example, a light source device can be integrated therein to provide light stimulation, and can be a grating, or a specific color carried thereon; the size of the septum 122 is adapted to the extent of its insertion site.
The use mode of the invention is as follows: in the social test experiment of the fish, one quadrant area (the other three quadrant areas are open spaces) is selected to place the zebra fish, the target fish is in the experiment target observation area 11, and the behavior of the target fish in a certain time is recorded. In the data analysis, the time of the fish in the vicinity of their fellow (social circle) was calculated as a response to the same kind of visual stimuli. Similarly, the influence of information on the target fish is tested by inserting a spacer 122 carrying information in a quadrant region and recording the behavior of the target fish.
It should be noted that: the baffle 121 can also divide the experimental environment configuration area 12 into halves and eights, and preferably, the baffle 121 can also be detachably connected, that is, clamped with the cavity body 1 and the partition plate 13; the cavity body 1 may also be square, and the corresponding experimental target observation area 11 may be circular or square; the spacer 122 may also be a waterproof display screen to facilitate study of relevant display stimuli; preferably, the height of the partition plate 13, the baffle plate 121 and the spacer 122 is not lower than the depth of the chamber body, or not lower than the depth of the water level.
Example 2
Referring to fig. 3 and 4, fig. 3 is a top view of another novel zebra fish behavioral experiment vessel according to the present invention; FIG. 4 is a schematic perspective view of another novel zebra fish behavioral experiment vessel of the invention. This embodiment is substantially the same as embodiment 1, except that the first connecting portion 1211 is a slot penetrating through the baffle 121, the spacer 122 includes not only a circular arc piece, but also a complete ring detachably connected to the slot, which has the advantages that the ring can rotate around the axis of the cavity body 1, for example, in an escape response experiment, a strip or a pattern of a certain color is coated on the spacer 122 and rotates, when the strip stimulus appears in the visual field of the zebra fish, the zebra fish can quickly determine whether to swim away from the area to avoid the stimulus, if the zebra fish is accustomed to the certain color, the spacer 122 with another color stimulus can be replaced, and the optical intensity of the stimulus can be adjusted, etc., so as to detect the excitation threshold of the zebra fish escape response, it should be noted that the first connecting portion 1211 described in this embodiment can also be applied to embodiment 1, used together with the circular arc sheet.
Example 3
Referring to fig. 5 and fig. 6, fig. 5 is a schematic view of another novel zebra fish behavioral experiment vessel according to the present invention; FIG. 6 is a schematic diagram of the matching of a vessel and a bumping post for a novel zebra fish behavioural experiment of the invention. The present embodiment is substantially the same as embodiment 1, and the difference is that the bottom of the central region of the cavity body 1 located in the experimental target observation area 11 is further provided with an installation portion 14, the novel vessel for zebra fish behavioural experiments further comprises a barrier column 15, and the height of the barrier column 15 is higher than or equal to the depth of the cavity body, or the height of the barrier column is not lower than the depth of the water level; the stop column 15 is detachably connected with the mounting part 14 to isolate the central area of the stop column, and the mounting part 14 is a mounting groove; preferably, the mounting portion 14 is provided with a plurality of retaining posts 15, i.e. retaining posts with different diameters, and correspondingly, the retaining posts 15 have different size ranges, such as cylinders with different diameters; the handling of the pillars 15 can be used for the study of exposed and non-exposed regions.
Example 4
Referring to fig. 7, fig. 7 is a schematic view of a cross-like maze shape of a novel vessel for zebra fish behavioral experiments. For further improving the flexibility of the device, the partition 13 is detachably connected to the chamber body 1, and a groove-shaped structure may also be disposed at the bottom of the chamber body 1 for clamping, etc., which has the advantages of forming a circular maze similar to a cross-shaped maze and being capable of expanding the range of the experimental target observation area 11, for example, the partition 122 is used as the boundary of the experimental target observation area 11, and correspondingly, in some induction experiments or social experiments, the partition 122 may be made of a transparent material; it should be noted that, the novel vessel for zebra fish behavioural experiments of the present invention can flexibly set the spacer 122, the partition 13, and the baffle 121 according to the experimental needs, for example, the sidewall of the cavity body 1 is provided with a plurality of groove-shaped structures adapted to the baffle 121 for the baffle 121 to be detachably connected to, the bottom of the cavity body 1 can be provided with groove-shaped structures adapted to the size and the installation direction of the baffle 121, the clamping between the assemblies belongs to a conventional clamping structure, including but not limited to the clamping groove structure shown in the drawings, and it should be understood that, because of the experiment of the fish body in water, the staggered groove-shaped structures in the cavity body 1 do not affect the experiment, and are beneficial to the installation of various structures.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and additions can be made without departing from the principle of the present invention, and these should also be considered as the protection scope of the present invention.
Claims (10)
1. A novel zebra fish vessel for behavioural experiments is characterized by comprising a cavity body, wherein the cavity body comprises an experiment target observation area and an experiment environment configuration area; the experimental target observation area and the experimental environment configuration area are separated by a transparent partition plate, the experimental target observation area is arranged in the center of the cavity body, and the experimental environment configuration area surrounds the experimental target observation area; the experimental environment configuration area comprises a baffle plate and a spacer; the baffle is used for partitioning the experiment environment configuration area, and the spacer is detachably connected with the baffle and used for changing the area environment.
2. The novel zebrafish behavioral experimental vessel according to claim 1, wherein the baffle is perpendicular to the side walls of the experimental target observation area and the experimental environment configuration area, and the baffle is detachably connected to the cavity body.
3. The novel zebrafish behavioral laboratory vessel according to claim 2, wherein the baffle plate bisects the experimental environment configuration zone.
4. The novel zebrafish behavioral experimental vessel according to claim 1, characterized in that the baffle is provided with a plurality of first connecting portions thereon, and the first connecting portions are detachably connected with the spacer.
5. The novel zebrafish behavioral experimental vessel according to claim 4, characterized in that, the first connecting portion is a slot structure, and the spacer is detachably connected with the adjacent baffle.
6. The novel zebrafish behavioral laboratory vessel according to claim 4, wherein the first connecting portion is a clamping groove penetrating through the baffle, and the spacer is movable along the clamping groove.
7. The novel zebrafish behavioral experimental vessel according to claim 1, characterized in that the bottom of the central region of the cavity body in the experimental target observation area is further provided with an installation part, and the installation part is provided with a stop column detachably connected with the installation part.
8. The novel zebrafish behavioral experimental vessel according to claim 7, wherein the mounting portion and the stopper are provided in a plurality of numbers and have different sizes.
9. The novel zebrafish behavioral experimental vessel according to claim 1, characterized in that the partition plate is detachably connected with the cavity body.
10. The novel zebrafish behavior experiment vessel according to any one of claims 1 to 9, wherein the spacer is used for carrying information, and can be a grating, a color stripe or an integrated LED display lamp, and the spacer can also be a display screen.
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| CN202111326490.0A CN113940306B (en) | 2021-11-10 | 2021-11-10 | Novel vessel for zebra fish behaviouristics experiments |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115399279A (en) * | 2022-09-23 | 2022-11-29 | 苏州大学 | Novel laboratory zebra fish autism ethology model device |
| CN116036440A (en) * | 2023-01-17 | 2023-05-02 | 北京智源人工智能研究院 | Visual stimulus experimental device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115399279A (en) * | 2022-09-23 | 2022-11-29 | 苏州大学 | Novel laboratory zebra fish autism ethology model device |
| CN116036440A (en) * | 2023-01-17 | 2023-05-02 | 北京智源人工智能研究院 | Visual stimulus experimental device |
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