CN116035072A - Application of Aronia melanocarpa extract in preparing anti-glycation camel milk powder food - Google Patents
Application of Aronia melanocarpa extract in preparing anti-glycation camel milk powder food Download PDFInfo
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- CN116035072A CN116035072A CN202211651595.8A CN202211651595A CN116035072A CN 116035072 A CN116035072 A CN 116035072A CN 202211651595 A CN202211651595 A CN 202211651595A CN 116035072 A CN116035072 A CN 116035072A
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Images
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-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2240/00—Use or particular additives or ingredients
- A23C2240/15—Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to application of a Aronia melanocarpa extract in preparing anti-saccharification camel milk powder food, in particular to anti-saccharification camel milk powder containing the Aronia melanocarpa extract, and belongs to the field of food. An anti-glycation camel milk powder containing Aronia melanocarpa extract, wherein the camel milk powder comprises Aronia melanocarpa extract. The Aronia melanocarpa extract is prepared by the following steps: pulping Aronia melanocarpa fruit, filtering, collecting filtrate, adding ethanol solution, extracting under ultrasonic assistance, filtering, collecting filtrate, evaporating, and lyophilizing to obtain Aronia melanocarpa extract powder. According to the invention, the Aronia melanocarpa extract is used as an anti-saccharification agent to be added into the camel milk powder, so that the content of AGEs endogenously formed in the processing process and after ingestion of the camel milk powder can be remarkably reduced, the quality of the camel milk powder is not influenced, and a way with wide prospects is provided for comprehensive application and development of Aronia melanocarpa.
Description
Technical Field
The invention relates to application of a Aronia melanocarpa extract in preparing anti-saccharification camel milk powder food, in particular to anti-saccharification camel milk powder containing the Aronia melanocarpa extract, and belongs to the field of food.
Background
Aronia melanocarpa (Aronia melanocarpa) is of Rosaceae, belonging to the family of Bush berry tree, also known as fructus Rosae Davuricae and Aronia melanocarpa. The fruits are rich in various components beneficial to human health, such as flavone, polyphenol, anthocyanin and the like, and are widely applied to the fields of medicine, functional food industry and the like in European and American countries. The introduction of Aronia melanocarpa in China since the 90 th century has been currently classified as one of the ten-sized berries emerging in China.
Camel milk is one of the living body utilization modes of camel, and a great deal of records on diseases of camel milk are recorded in history. Commercial camel milk originates in china and in recent years, the yield of national camel milk is gradually increasing. The fresh camel milk is processed into camel milk powder which is easier to store and convenient to eat, and the camel milk powder generates advanced glycosylation end products due to classical Maillard reaction in the processing process. Advanced glycation end products (Advanced glycation end products, AGEs) are stable, irreversible compounds formed by a series of complex reactions such as rearrangement, dehydration, oxidation, condensation, etc. between carbonyl groups of reducing sugars (e.g., glucose) and amino groups of nucleic acids, fatty acids, proteins, or amino acids. The formation route of AGEs mainly comprises (1) Maillard reaction and lipid peroxidation reaction in food processing process to generate exogenous AGEs. (2) Glucose and amino acid residues in human blood combine to produce endogenous AGEs. Research shows that long-term and massive accumulation of exogenous or endogenous AGEs in the body can induce related chronic diseases, such as diabetes, alzheimer's disease, cardiovascular diseases, kidney diseases, nervous system diseases and the like. Generally, any step of blocking the formation of AGEs can achieve the effect of inhibiting the formation of AGEs. Therefore, the potential inhibition mechanism of AGEs inhibitors is mainly to reduce oxidation and oxidative stress of saccharides by blocking adhesion of saccharides to proteins, capturing or scavenging alpha-dicarbonyl compounds, free radicals, nitrogen-containing substances and other intermediates generated in the process of partial saccharification, thereby blocking formed AGEs cross-links. Aminoguanidine has a good inhibitory effect on AGEs, but is unsuitable for adding to milk powder in view of the problems of safety, side effects and the like of synthetic drugs. It is particularly important to find natural products from nature that have the effect of inhibiting advanced glycation end products.
Disclosure of Invention
The invention aims at the application of the Aronia melanocarpa extract in preparing the anti-glycation camel milk powder food. By establishing different in-vitro non-enzymatic glycosylation reaction simulation systems, the chokeberry extract is used as a natural inhibitor, and aminoguanidine is used as a positive control to discuss the inhibition effect of the chokeberry extract on AGEs.
The invention provides an application of Aronia melanocarpa extract as an anti-glycation inhibitor. Further provides an application of the Aronia melanocarpa extract as an anti-glycation inhibitor in preparing anti-glycation camel milk powder food.
The application of Aronia melanocarpa extract in preparing anti-glycation camel milk powder food is provided.
The Aronia melanocarpa camel milk powder can reduce the exogenous AGEs generation content and achieve the anti-saccharification effect.
An anti-glycation camel milk powder comprising an extract of Aronia melanocarpa.
Preferably, the Aronia melanocarpa extract is prepared by the following method: pulping Aronia melanocarpa fruit, filtering, collecting filtrate, adding 70% ethanol solution at a feed-liquid ratio of 1:40g/mL, extracting with ultrasonic wave at 45deg.C and ultrasonic frequency of 600W for 90min, and repeating above steps for several times; cooling to room temperature, filtering, collecting filtrate, evaporating, and lyophilizing to obtain Aronia melanocarpa extract powder.
Further, the Aronia melanocarpa extract is prepared by the following method: the Aronia melanocarpa extract is prepared by the following steps: picking up mature Aronia melanocarpa fruits, cleaning, pulping, filtering, collecting filtrate, adding 70% ethanol solution with a feed-liquid ratio of 1:40g/mL, extracting with ultrasonic wave at 45deg.C and ultrasonic frequency of 600W for 90min, and repeating above steps for three times; cooling to room temperature, filtering, collecting filtrate, rotary evaporating, lyophilizing to obtain Aronia melanocarpa extract powder, and collecting and storing at-80deg.C.
The anti-saccharification camel milk powder consists of the following components in parts by mass: the camel milk powder consists of the following components in parts by mass: 30-40 parts of defatted camel milk powder, 10-20 parts of whole camel milk powder, 10-20 parts of concentrated whey protein powder, 5-10 parts of resistant dextrin, 1-5 parts of erythritol, 1-5 parts of fructo-oligosaccharide, 1-5 parts of Aronia melanocarpa extract and 2-8 parts of nutrient substances.
Preferably, the camel milk powder consists of the following components in parts by mass: 35 parts of defatted camel milk powder, 15 parts of full-fat camel milk powder, 15 parts of concentrated whey protein powder, 8 parts of resistant dextrin, 3 parts of erythritol, 3 parts of fructo-oligosaccharide, 5 parts of Aronia melanocarpa extract and 6 parts of nutrient substances.
Further, the nutrient substances in the milk powder consist of the following nutrient components in parts by mass: 0.1 part of vitamin A, 0.02 part of vitamin D, 0.15 part of vitamin E and vitamin B 6 0.1 part of vitamin C1 part, folic acid 0.03 part, calcium carbonate 3 parts, magnesium oxide 1 part, ferrous sulfate 0.3 part and zinc oxide 0.3 part.
The invention also aims to provide a preparation method of the anti-glycation camel milk powder, which comprises the following steps: the raw materials are respectively added with water for uniform dissolution, and the water dissolution ratio is 1:15, sequentially feeding the materials into a high-speed shearing tank for mixing; preheating to 60-65 ℃ before homogenizing, homogenizing under 20-22 Mpa, sterilizing for 4s at 95-100 ℃ after homogenizing, and vacuum concentrating to 23-27% of the volume of mixed milk, wherein the mass fraction of solid matters in the milk is 30-40%; high-pressure spray drying is carried out in a drying tower, the air inlet temperature is set to be 180-200 ℃, and the air exhaust temperature is set to be 70-80 ℃; and quickly cooling the milk powder to room temperature to obtain the finished milk powder.
The beneficial effects of the invention are as follows: according to the invention, the Aronia melanocarpa extract is used as an anti-saccharification agent to be added into camel milk powder, so that the content of AGEs endogenously formed in the processing process and after intake of camel milk powder can be remarkably reduced, and meanwhile, the quality of camel milk powder is not influenced. The Aronia melanocarpa extract has good anti-saccharification activity, can be used as a natural food additive raw material to be applied to camel milk powder food, and provides a way with wide prospects for comprehensive application and development of Aronia melanocarpa.
Drawings
FIG. 1 shows the inhibition ratio of Aronia melanocarpa extract to total fluorescence AGEs formation in different in vitro simulated non-enzymatic glycosylation reaction systems. (A) bovine serum albumin-fructose reaction system. (B) bovine serum albumin-methylglyoxal reaction system. (C) bovine serum albumin-glyoxal reaction system.
FIG. 2 shows the inhibitory effect of Aronia melanocarpa extract at different concentrations on fluorescent AGEs (A.37 ℃,7 days, B.100 ℃,60 min) in camel milk.
Note that: the plots indicate significant differences between the comparison groups within the 95% confidence interval, indicating P <0.0001; the letters in the figure represent significant differences between the comparison groups within the 95% confidence interval, P <0.05; statistical calculations were performed using analysis of variance ANOVA.
Detailed Description
The following non-limiting examples will enable those of ordinary skill in the art to more fully understand the invention and are not intended to limit the invention in any way.
The test methods described in the following examples, unless otherwise specified, are all conventional; the reagents and materials, unless otherwise specified, are commercially available.
Example 1
Mature Aronia melanocarpa fruits are picked, cleaned, 250g of fruits are weighed, 500mL of purified water is added into the fruits, and 300g of filtrate is obtained through pulping, filtering and collecting the fruit residues. Adding 70% ethanol solution according to the feed liquid ratio of the filtrate to the ethanol solution of 1:40g/mL, and carrying out ultrasonic-assisted extraction for 90min at 45 ℃ under the ultrasonic frequency of 600W, wherein the above operation is repeated three times; cooling to room temperature, filtering, collecting filtrate, rotary evaporating, lyophilizing to obtain Aronia melanocarpa extract powder, and collecting and storing at-80deg.C.
The anti-saccharification camel milk powder is prepared by the following steps:
(1) Weighing the raw materials: weighing the raw materials for standby.
Feeding and mixing: 35 parts of defatted camel milk powder, 15 parts of whole camel milk powder, 15 parts of concentrated whey protein powder, 8 parts of resistant dextrin, 3 parts of erythritol, 3 parts of fructo-oligosaccharide, 5 parts of black chokeberry extract and 6 parts of nutrient substances are added with water to be dissolved uniformly (water dissolution ratio is 1:15), and the mixture is sequentially fed into a high-speed shearing tank for mixing, wherein the nutrient substances comprise the following nutrient components in parts by mass: 0.1 part of vitamin A, 0.02 part of vitamin D, 0.15 part of vitamin E and vitamin B 6 0.1 part of vitamin C1 part, folic acid 0.03 part, calcium carbonate 3 parts, magnesium oxide 1 part, ferrous sulfate 0.3 part and zinc oxide 0.3 part.
Homogenizing and concentrating: preheating to 65deg.C, homogenizing under 20Mpa, sterilizing at 100deg.C for 4s, vacuum concentrating to 25% of the volume of mixed milk, and mixing with 35% of solid matter.
(2) Spray drying: high-pressure spray drying is carried out in a drying tower, the air inlet temperature is set to be 200 ℃, and the air exhaust temperature is set to be 75 ℃.
(3) Dry blending: and quickly cooling the milk powder to room temperature to obtain the finished milk powder.
Establishing an in vitro simulated non-enzymatic glycosylation reaction system:
(1) Establishment of bovine serum albumin-fructose reaction system
1mL BSA solution (20 mg/mL), 1mL fructose solution (0.5 mol/L) was mixed with 1mL Aronia melanocarpa extract aqueous solution (0.1, 0.5,1.0,5.0 mg/mL) of example 1, and all the reactants were dissolved in 0.2mol/L phosphate buffer (pH=7.4). Aminoguanidine (AG, 0.1 mg/mL) solution was used as a positive control, and the glycated protein solution was used as a blank, and cultured in a constant temperature incubator at 37℃for 7d.
(2) Establishment of bovine serum albumin-methylglyoxal reaction system
1mL BSA solution (20 mg/mL), 1mL methylglyoxal solution (10 mol/L) and 1mL Aronia melanocarpa extract solution (0.1, 0.5,1.0,5.0 mg/mL) were mixed and all the reactants were dissolved in phosphate buffer (0.2 mol/L pH=7.4). Aminoguanidine (AG, 0.1 mg/mL) solution was used as a positive control, and the glycated protein solution was used as a blank, and cultured in a constant temperature incubator at 37℃for 7d.
(3) Establishment of bovine serum albumin-glyoxal reaction system
1mL BSA solution (20 mg/mL), 1mL glyoxal solution (10 mol/L) and 1mL Aronia melanocarpa extract solution (0.1, 0.5,1.0,5.0 mg/mL) were mixed and all the reactants were dissolved in phosphate buffer (0.2 mol/L pH=7.4). Aminoguanidine (AG, 0.1 mg/mL) solution was used as a positive control, and the glycated protein solution was used as a blank, and cultured in a constant temperature incubator at 37℃for 7d.
(4) Determination of Total AGEs
After 7d, 1.5mL of the reaction solution was aspirated, and the fluorescence intensity was measured at an excitation wavelength of 370nm and an emission wavelength of 440 nm. The inhibition rate of Aronia melanocarpa to AGEs was calculated as follows:
inhibition (%) = (F) 0 -F)/F 0 ×100%
Note that: f (F) 0 The fluorescence intensity of the blank group is F, and the fluorescence intensity of the experimental group is F.
The inhibition ability of the Aronia melanocarpa extract on endogenous fluorescent AGEs in camel milk powder was determined. 1g of camel milk powder is weighed, 20mL of ultrapure water is added into the milk powder, and a glass rod is stirred until no particles exist. And (3) dispersing the small beaker containing the liquid to be tested at a high speed (10000 r/min,1 min). After the crushing is finished, the liquid to be detected is magnetically stirred (150 r,30 min). Adding Aronia melanocarpa extract with different concentrations into camel emulsion in equal volume, mixing, heating in 100deg.C water bath for 10min, incubating in 37 deg.C constant temperature incubator for 7 days in dark place, taking out appropriate amount of sample solution, and measuring fluorescence intensity of sample under excitation wavelength of 370nm and emission wavelength of 440 nm. The inhibition rate of the Aronia melanocarpa extract with different concentrations on endogenous fluorescent AGEs in camel milk powder is calculated according to the following formula:
inhibition (%) = (F) 0 -F)/F 0 ×100%
The inhibition ability of the Aronia melanocarpa extract on exogenous fluorescent AGEs in camel milk powder is measured. 1g of camel milk powder is weighed, 20mL of ultrapure water is added into the milk powder, and a glass rod is stirred until no particles exist. And (3) dispersing the small beaker containing the liquid to be tested at a high speed (10000 r/min,1 min). After the crushing is finished, the liquid to be detected is magnetically stirred (150 r,30 min). Adding the Aronia melanocarpa extract with different concentrations into camel emulsion in equal volume, mixing, heating in water bath at 100deg.C for 60min, cooling, taking out appropriate amount of sample solution, and measuring fluorescence intensity under excitation wavelength of 370nm and emission wavelength of 440 nm. The inhibition rate of the Aronia melanocarpa extract with different concentrations on the external fluorescence AGEs in camel milk powder is calculated according to the following formula:
inhibition (%) = (F) 0 -F)/F 0 ×100%
TABLE 1 inhibition ratio (%)
TABLE 2 inhibition of endogenous and exogenous fluorescent AGEs by Aronia melanocarpa extract (%)
Claims (8)
1. The application of Aronia melanocarpa extract in preparing anti-glycation camel milk powder food is provided.
2. An anti-glycation camel milk powder containing a black fruit Aronia melanocarpa extract is characterized in that: the camel milk powder comprises the Aronia melanocarpa extract.
3. The anti-glycation camel milk powder according to claim 2, characterized in that: the Aronia melanocarpa extract is prepared by the following steps: pulping Aronia melanocarpa fruit, filtering, collecting filtrate, adding 70% ethanol solution at a feed-liquid ratio of 1:40g/mL, extracting with ultrasonic wave at 45deg.C and ultrasonic frequency of 600W for 90min, and repeating above steps for several times; cooling to room temperature, filtering, collecting filtrate, evaporating, and lyophilizing to obtain Aronia melanocarpa extract powder.
4. An anti-glycation camel milk powder according to claim 3, characterized in that: the Aronia melanocarpa extract is prepared by the following steps: picking up mature Aronia melanocarpa fruits, cleaning, pulping, filtering, collecting filtrate, adding 70% ethanol solution with a feed-liquid ratio of 1:40g/mL, extracting with ultrasonic wave at 45deg.C and ultrasonic frequency of 600W for 90min, and repeating above steps for three times; cooling to room temperature, filtering, collecting filtrate, rotary evaporating, lyophilizing to obtain Aronia melanocarpa extract powder, and collecting and storing at-80deg.C.
5. The anti-glycation camel milk powder according to claim 2, characterized in that: the camel milk powder consists of the following components in parts by mass: 30-40 parts of defatted camel milk powder, 10-20 parts of whole camel milk powder, 10-20 parts of concentrated whey protein powder, 5-10 parts of resistant dextrin, 1-5 parts of erythritol, 1-5 parts of fructo-oligosaccharide, 1-5 parts of Aronia melanocarpa extract and 2-8 parts of nutrient substances.
6. The anti-glycation camel milk powder of claim 5, characterized in that: the camel milk powder consists of the following components in parts by mass: 35 parts of defatted camel milk powder, 15 parts of full-fat camel milk powder, 15 parts of concentrated whey protein powder, 8 parts of resistant dextrin, 3 parts of erythritol, 3 parts of fructo-oligosaccharide, 5 parts of Aronia melanocarpa extract and 6 parts of nutrient substances.
7. Anti-glycation camel milk powder according to claim 5 or 6, characterized in that: the nutrient substances in the milk powder consist of the following nutrient components in parts by mass: 0.1 part of vitamin A, 0.02 part of vitamin D, 0.15 part of vitamin E and vitamin B 6 0.1 part of vitamin C1 part, folic acid 0.03 part, calcium carbonate 3 parts, magnesium oxide 1 part, ferrous sulfate 0.3 part and zinc oxide 0.3 part.
8. The anti-glycation camel milk powder of claim 5, characterized in that: the anti-saccharification camel milk powder is prepared by the following method,
the raw materials are respectively added with water for uniform dissolution, and the water dissolution ratio is 1:15, sequentially feeding the materials into a high-speed shearing tank for mixing; preheating to 60-65 ℃ before homogenizing, homogenizing under 20-22 Mpa, sterilizing for 4s at 95-100 ℃ after homogenizing, and vacuum concentrating to 23-27% of the volume of mixed milk, wherein the mass fraction of solid matters in the milk is 30-40%; high-pressure spray drying is carried out in a drying tower, the air inlet temperature is set to be 180-200 ℃, and the air exhaust temperature is set to be 70-80 ℃; and quickly cooling the milk powder to room temperature to obtain the finished milk powder.
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