CN116008577A - TIP sample addition abnormality detection method and detection device based on pressure detection - Google Patents
TIP sample addition abnormality detection method and detection device based on pressure detection Download PDFInfo
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Abstract
The invention discloses a TIP sample addition abnormality detection method and device based on pressure detection, and relates to the technical field of in-vitro diagnosis medical detection instruments. The TIP sample addition abnormality detection method based on pressure detection comprises the steps of collecting a pressure value in a sampling needle as comparison data when the sampling needle does not work; collecting a real-time pressure value in the needle when the sampling needle works; comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state; and outputting a signal of the working state. The TIP sample adding abnormality detection method based on pressure detection detects the whole working process of the sampling needle, is convenient for grasping the working state of the whole process of liquid suction and liquid injection of the sampling needle, is convenient for discriminating the working reason of the sampling needle in an abnormal state, takes accurate rescue measures, saves time and labor, reduces cost, and is suitable for a TIP sample adding system by collecting the pressure value in the needle as comparison data when the sampling needle works each time.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis medical detection instruments, in particular to a TIP sample addition abnormality detection method based on pressure detection. In addition, the invention also relates to a detection device capable of executing the TIP sample addition abnormality detection method based on pressure detection. In addition, the invention also relates to a detection device capable of executing the TIP sample addition abnormality detection method based on pressure detection.
Background
When a sample is manufactured, a sampling needle and a system thereof are usually detected, so that whether the manufactured sample is qualified or not is obtained, and one widely used detection method is to compare the pressure value of the liquid suction process measured by using a pressure sensor with a threshold value obtained by automatic calibration, so as to judge whether the liquid suction process is abnormal or not, and further whether the sample is qualified or not can be known, but the pressure sensor is greatly influenced by environmental factors such as external temperature and the like, and the test result is inaccurate.
In summary, how to improve the accuracy of the sample loading detection result is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the present invention aims to provide a pressure detection-based TIP sampling abnormality detection method, which detects the whole working process of a sampling needle, is convenient for grasping the working state of the whole process of liquid suction and liquid injection of the sampling needle, is convenient for discriminating the cause of working in an abnormal state of the sampling needle, makes accurate rescue measures, saves time and labor, and reduces cost.
Another object of the present invention is to provide a detection apparatus capable of performing the above TIP loading anomaly detection method based on pressure detection.
It is still another object of the present invention to provide another detecting device capable of performing the above-mentioned TIP loading abnormality detecting method based on pressure detection.
In order to achieve the above object, the present invention provides the following technical solutions:
a TIP sample addition abnormality detection method based on pressure detection comprises the following steps:
collecting a pressure value in the needle as comparison data when the sampling needle does not work;
collecting a real-time pressure value in the sampling needle when the sampling needle works;
comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state;
and outputting the signal of the working state.
Preferably, when the sampling needle aspirates, the sampling needle comprises:
collecting the pressure value in the sampling needle as first contrast data before each imbibition;
in the imbibition process, collecting the real-time pressure value and drawing into an imbibition pressure curve;
selecting a first data interval from the imbibition pressure curve;
taking absolute values of the real-time pressure values of the first data interval and the first comparison data respectively, and taking corresponding first absolute difference values;
summing the first absolute difference values to obtain a first parameter;
comparing the first parameter with a preset first threshold value and a preset second threshold value,
when the first parameter is smaller than the first threshold, judging that the sampling needle is in a suction working state, and outputting a signal for alarming suction;
when the first parameter is greater than or equal to the first threshold value and the first parameter is less than or equal to the second threshold value, judging that the sampling needle is in a working state of normal liquid suction, and outputting a signal of normal liquid suction;
when the first parameter is larger than the second threshold, judging that the sampling needle is in a working state of needle blocking, and outputting a signal for alarming the needle blocking.
Preferably, the imbibition pressure curve is divided into an imbibition starting section, a stable imbibition section and an imbibition stopping section, and the first data interval is positioned in the stable imbibition section.
Preferably, when the judgment result is that the needle is blocked, the method further comprises:
sucking, beating and uniformly mixing the sample;
and controlling the sampling needle to suck liquid again, and collecting the real-time pressure value and drawing the real-time pressure value into a liquid sucking pressure curve in the liquid sucking process.
Preferably, when the sampling needle is injected, the method comprises:
in the liquid injection process, collecting the real-time pressure value and drawing to form an injection pressure force curve;
after each injection is completed, collecting the pressure value of the sampling needle after the internal pressure is stabilized as second comparison data;
selecting a section after the pressure peak value from the injection pressure force curve as a second data section;
taking absolute values of the real-time pressure values of the second data interval and the second comparison data respectively, and obtaining corresponding second absolute difference values;
summing the second absolute differences to obtain a second parameter;
comparing the second parameter with a preset third threshold value and a preset fourth threshold value,
when the second parameter is greater than or equal to the third threshold value and the second parameter is less than or equal to the fourth threshold value, judging that the sampling needle is in a working state of normal liquid injection, and outputting a signal of normal liquid injection;
and when the second parameter is smaller than the third threshold value or the second parameter is larger than the fourth threshold value, judging that the sampling needle is in a non-adding working state, and outputting a signal for alarming the non-adding.
Preferably, the pressure peak value in the injection pressure force curve is acquired;
the pressure peak value and the second comparison data are subjected to difference to obtain a third parameter;
when judging that the liquid is normally injected, comparing the third parameter with a preset fifth threshold value,
and if the third parameter is smaller than the fifth threshold, judging that the sampling needle is in an abnormal liquid injection working state, and outputting an abnormal liquid injection signal.
Preferably, when the second parameter is greater than the fourth threshold, the sampling needle is judged to be in a working state of sample viscosity, and a sample viscosity signal is output;
comparing the second comparison data with a preset range when the second parameter is smaller than the third threshold value,
if the second comparison data is larger than the preset range, judging that the sampling needle is in a needle blocking working state, and outputting a needle blocking signal;
and if the second comparison data is smaller than or equal to the preset range, judging that the sampling needle is in the working state of empty injection, and outputting an empty injection signal.
Preferably, when the judging result is the working state of the abnormal filling, the method further comprises:
and (3) carrying out liquid suction again to carry out filling, and collecting the real-time pressure value and drawing the real-time pressure value into a liquid injection pressure curve in the liquid injection returning process.
A detection apparatus, comprising:
a sampling needle;
the pressure sensor is used for collecting the pressure value inside the sampling needle as comparison data when the sampling needle does not work and is used for collecting the real-time pressure value inside the sampling needle when the sampling needle works;
and the controller is used for comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state, and outputting a signal of the working state.
The detection device comprises a controller and an actuator, wherein the controller stores the TIP sample addition abnormality detection method based on pressure detection provided by any one of the above, and is connected with the actuator and used for controlling the actuator to execute the step of the TIP sample addition abnormality detection method based on pressure detection.
According to the TIP sample addition abnormality detection method based on pressure detection, when a sampling needle does not work, the pressure value in the needle is collected as comparison data; in the step, when the sampling needle does not work, the method refers to the steps that before the instrument debugging is finished and the liquid suction operation is not performed, and after the liquid injection of the sampling needle is finished and the original position is recovered; continuously collecting pressure values in the needle in the time period to obtain stable pressure values in the needle in a period of time; the comparison data can be an actual collected pressure value, preferably, the collected pressure value is averaged to be used as the comparison data, so that the influence of factors such as unstable instruments or environment can be reduced, and the comparison data can be obtained to prepare for later data processing
Collecting a real-time pressure value in the needle when the sampling needle works; the working time of the sampling needle in the step means that the stage of adjusting the sampling needle before liquid suction starts, the real-time pressure value inside the sampling needle in the period is collected after liquid injection is completed, further preparation is made for later data processing, and preferably, the pressure value is collected every N milliseconds, so that uniform and more real-time pressure values are obtained.
Comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state; the data obtained in the previous two steps are processed, the real-time pressure value is compared with the comparison data, the real-time pressure value can be directly compared with the comparison data, or the real-time pressure value is further processed and then compared with the comparison data, so that the working state of the sampling needle in the liquid suction and injection stages is judged.
Outputting a signal of the working state; the step realizes information interaction, outputs the working state of the sampling needle corresponding to the data processing result, and presents the working state to an operator, so that the operator or an actuator can perform further processing.
According to the TIP sample addition abnormality detection method based on pressure detection, the whole working process of the sampling needle can be detected, the working state of the sampling needle in the liquid suction or liquid injection process can be mastered conveniently, when the sampling needle works in an abnormal state can be known clearly, the reason of the working of the sampling needle in the abnormal state can be distinguished conveniently, accurate rescue measures are made, time and labor are saved, and cost is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flowchart of a judging method according to a first embodiment of the present invention;
fig. 2 is a flowchart of a liquid suction judging method according to a second embodiment of the present invention;
fig. 3 is a flowchart of a liquid suction judging method according to a third embodiment of the present invention;
FIG. 4 is a flow chart of a method for determining injection according to a fourth embodiment of the present invention;
FIG. 5 is a flow chart of a fifth embodiment of a method for determining injection according to the present invention;
FIG. 6 is a flow chart of a method for determining injection according to a sixth embodiment of the present invention;
FIG. 7 is a flow chart of a method for determining injection according to a seventh embodiment of the present invention;
FIG. 8 is a graph of suction pressure for an embodiment of the present invention;
FIG. 9 is a graph of the pressure of the injection liquid according to an embodiment of the present invention;
fig. 10 is a schematic structural diagram of a detection device according to an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention has the core of providing a TIP sample addition abnormality detection method based on pressure detection, which detects the whole working process of a sampling needle, is convenient for grasping the working state of the whole process of liquid suction and liquid injection of the sampling needle, is convenient for discriminating the working reason of the sampling needle in an abnormal state, makes accurate rescue measures, saves time and labor and reduces cost. Another core of the present invention is to provide a detection device capable of executing the above TIP loading anomaly detection method based on pressure detection. Another core of the present invention is to provide another detection device capable of executing the above-mentioned TIP loading anomaly detection method based on pressure detection.
Referring to fig. 1 to 10, the present invention provides a method for detecting TIP loading anomalies based on pressure detection, comprising the steps of:
step S1, collecting a pressure value in a sampling needle as comparison data when the sampling needle does not work;
s2, collecting a real-time pressure value in the needle when the sampling needle works;
s3, comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state;
and S4, outputting a signal of the working state.
Specifically, as shown in fig. 1, in step S1, when the sampling needle does not work, it means before the instrument debugging is completed and the liquid sucking operation is not performed yet, and after the liquid injection of the sampling needle is completed and the original position is recovered; continuously collecting pressure values in the needle in the time period to obtain stable pressure values in the needle in a period of time; the comparison data can be an actually collected pressure value, and preferably, the collected pressure value is averaged to be used as the comparison data, so that the influence of factors such as instability of an instrument or environment can be reduced, and the comparison data is obtained to prepare for later data processing.
In step S2, the working time of the sampling needle means that the stage of adjusting the sampling needle before imbibition starts, and after the injection is completed, the real-time pressure value inside the sampling needle in the above period of time is collected, so that further preparation is made for the later data processing, preferably, the pressure value is collected every N milliseconds, so that even and more real-time pressure values are obtained. It should be noted that, the method of collecting the pressure values in step S1 and step S2 is not limited, and the pressure values may be manually read and processed, or may be read and processed by a machine, or even the two methods may be combined, so long as the operator is informed of the required data.
In step S3, the data obtained in step S1 and step S2 are processed, the real-time pressure value is compared with the comparison data, and the real-time pressure value can be directly compared with the comparison data, or the real-time pressure value is further processed and then compared with the comparison data, so that the working state of the sampling needle in the liquid suction and injection stage is judged.
In step S4, information interaction is realized, the working state of the sampling needle corresponding to the data processing result is output and presented to an operator, so that the operator or an actuator can perform further processing conveniently.
The TIP sample addition abnormality detection method based on pressure detection can detect the whole working process of the sampling needle, is convenient for grasping the working state of the sampling needle in the process of liquid suction or liquid injection, can clearly know when the sampling needle works in an abnormal state, is further convenient for discriminating the reason of the sampling needle working in the abnormal state, takes accurate rescue measures, saves time and labor, reduces cost, and is particularly suitable for a TIP sample addition system by collecting the pressure value in the needle as comparison data when the sampling needle works each time.
Further, when the working state is a normal state, a qualified signal of the prepared sample can be output; and outputting a disqualification signal of the prepared sample when the working state is abnormal. Whether the sample manufactured by the sampling needle is qualified or not is directly output, so that an operator or a controller is prevented from further judging, sample manufacturing time is saved, and the error rate of qualified sample screening is reduced.
On the basis of the above embodiment, for the liquid sucking operation, the steps S1 to S4 specifically include the following steps:
step S11, collecting pressure values in a sampling needle before each imbibition as first comparison data;
step S12, in the imbibition process, collecting a real-time pressure value and drawing into an imbibition pressure curve;
s13, selecting a first data interval from the imbibition pressure curve;
step S14, taking absolute values of the real-time pressure values in the first data interval and the first comparison data to obtain corresponding first absolute difference values;
step S15, summing all the first absolute difference values to obtain a first parameter;
step S16, comparing the first parameter with a preset first threshold value and a preset second threshold value,
when the first parameter is smaller than a first threshold value, judging that the sampling needle is in a suction working state, and outputting a signal for alarming suction;
when the first parameter is larger than or equal to a first threshold value and the first parameter is smaller than or equal to a second threshold value, judging that the sampling needle is in a working state of normal liquid suction, and outputting a signal of normal liquid suction;
when the first parameter is larger than the second threshold, judging that the sampling needle is in a working state of needle blocking, and outputting a signal for alarming the needle blocking.
Specifically, as shown in fig. 2, in step S11, before the instrument is debugged and the operation of pipetting is not performed, in this time period, pressure values in a plurality of sampling needles are collected, and the collected pressure values are subjected to mean processing, or one of the pressure values is taken as first comparison data.
In the step S12, in the whole process of primary imbibition, a real-time pressure value is acquired according to time and is drawn into an imbibition pressure curve, the step can be subdivided into two steps to be executed, the acquisition of the real-time pressure value is finished firstly, and then operations such as recording, dotting, line drawing and the like are carried out according to the acquired data to prepare the imbibition pressure curve; the computer can also be used for collecting the real-time pressure value in the needle and drawing the imbibition pressure curve while sampling the imbibition of the needle.
In step S13, the first data interval is data collected in a suitable time period, and the selection of the first data interval is related to the final judgment result, and may be intercepted at any position in the liquid injection process of a liquid suction pressure curve, preferably, multiple experiments are performed to summarize rules, so as to obtain which part of the first data interval is selected in the liquid suction process.
In step S14, absolute values of the positive and negative deviations of the real-time pressure values selected in step S13 and the first comparison data selected in step S11 are taken to obtain a first absolute difference value.
In step S15, the first absolute difference values obtained in step S14 are summed up, and a first parameter is calculated; and the liquid suction pressure curve, the first data interval and the constant function line where the first comparison data are located can be enclosed into a closed graph, wherein the area of the closed graph is the curve integral of the liquid suction pressure curve, and the area of the closed graph is the same as the first parameter representation.
In step S16, a first threshold and a second threshold are preset according to the difference of the liquid characteristics and the liquid absorption amount of the sample, the first parameter calculated in step S15 is compared with the first threshold and the second threshold, and the sampling needle is judged to be in one of the working states of suction, normal suction or needle blocking, and a corresponding signal of alarm suction, normal suction or needle blocking is output.
On the one hand, positive and negative deviations between the first data interval and the first contrast data are summed after taking absolute values, so that the working state of normal liquid suction of the sampling needle is obtained by directly summing the positive and negative deviations, and compared with the situation that the real-time pressure value is directly compared with the first contrast data for judgment, the measuring result of the invention is more accurate; on the other hand, the first comparison data is collected before each liquid absorption, so that the influence of environmental factors such as temperature on a measurement result is effectively reduced.
Based on the above embodiment, the imbibition pressure curve is divided into an imbibition start section, a stable imbibition section and an imbibition stop section, and the first data interval is positioned in the stable imbibition section.
Specifically, as shown in fig. 8, the sections X1-X2 are the liquid suction starting section, the sections X2-X3 are the stable liquid suction section, the sections X3-X4 are the liquid suction stopping section, the sections X2-X3 are the empty suction state, the needle blocking state and the normal liquid suction state, the sections X2-X3 are selected as the first data section, the first parameter obtained by data processing has larger phase difference, possible pressure fluctuation of the liquid suction stopping section is avoided, the measured result is more accurate, the situation that the sampling needle cannot discriminate between the liquid suction starting section and the normal liquid suction state due to factors such as poor ventilation, position deviation after debugging can be avoided, and the accuracy of the detection result is improved.
On the basis of the above embodiment, when the determination result in step S16 is that the needle is blocked, the method further includes the following steps:
s17, sucking, beating and uniformly mixing the sample; the abnormal handling operation of suction and beating and mixing is specifically to discharge the liquid in the needle, suck the liquid again and discharge the liquid, and repeat the above operations to mix the liquid.
Step S18, controlling the sampling needle again to absorb liquid, and returning to the step S12; and the detection is carried out again, so that the probability of blocking the needle can be effectively reduced.
On the basis of the above embodiment, for the injection operation, the steps S1 to S4 specifically include the following steps:
step 21, in the liquid injection process, collecting a real-time pressure value and drawing the real-time pressure value into a liquid injection pressure curve;
step 22, after each injection is completed, collecting the pressure value of the sampling needle after the internal pressure is stabilized as second comparison data;
step 23, selecting a section after the pressure peak value as a second data section in the injection pressure force curve;
step 24, taking the absolute value of each real-time pressure value in the second data interval to be the corresponding second absolute difference value after respectively making difference with the second comparison data;
step 25, summing the second absolute differences to obtain a second parameter;
step 26, comparing the second parameter with a preset third threshold value and a preset fourth threshold value,
when the second parameter is larger than or equal to a third threshold value and the second parameter is smaller than or equal to a fourth threshold value, judging that the sampling needle is in a working state of normal liquid injection, and outputting a signal of normal liquid injection;
when the second parameter is smaller than the third threshold value or the second parameter is larger than the fourth threshold value, judging that the sampling needle is in a non-adding working state, and outputting a warning non-adding signal.
Specifically, as shown in fig. 4, in step 21, in the whole process from the beginning to the end of the liquid injection, the collected real-time pressure value is plotted with time as an independent variable, and a liquid injection pressure curve is obtained by plotting, as shown in fig. 9.
In step 22, after the sampling needle is restored to its original position after each injection, the pressure value in the needle is collected, that is, one or several pressure values in the section Y4-Y5 are collected, preferably, several pressure values in the sampling needle are collected, and the collected pressure values are subjected to mean value processing.
In step 23, in the injection pressure force curve, a section after the pressure peak is selected from the sections Y0-Y1 as the second data section, and preferably, a section with the most obvious difference from the peak to the bottom of the normal injection working state is selected.
In step 24, the second absolute difference is obtained by taking the absolute value of the positive and negative deviations of the real-time pressure values in the second data interval and the second comparison data selected in step 22.
In step 25, the second absolute differences obtained in step 24 are summed to calculate a second parameter. Similarly, the constant function line where the injection pressure force curve, the second data interval and the second comparison data are located is enclosed to form a closed graph, the area of the closed graph is the curve integral of the injection pressure force curve, the area of the closed graph is compared with the area of the closed graph of the normal injection of the sampling needle, and whether the sampling needle works normally in the injection process can be intuitively known in the injection pressure force curve as shown in fig. 9.
In step 26, a third threshold and a fourth threshold are preset according to the liquid characteristic difference of the sample, the liquid absorption amount and the like; and (3) comparing the second parameter obtained in the step (25) with a preset third threshold value and a preset fourth threshold value to obtain a working state of normal liquid injection or liquid leakage and addition, and correspondingly, outputting signals of normal liquid injection and liquid leakage and addition alarm.
On the one hand, positive and negative deviations between the second data interval and the second comparison data are summed after taking absolute values, so that the working state of normal liquid injection is avoided from being directly summed by the positive and negative deviations, and compared with the condition that the real-time pressure value is directly compared with the second comparison data for judgment, the result measured by the method is more accurate; on the other hand, the second comparison data are acquired after each liquid injection, so that the influence of environmental factors such as temperature on a measurement result is effectively reduced, and in the case of liquid suction for multiple liquid injection at one time, false alarm caused by different pressure values due to different residual liquid amounts in the needle can be avoided, and the accuracy of detection during liquid injection is improved.
On the basis of the above embodiment, when the judgment result in step 26 is that the addition is missing, the method further includes the following steps:
step 27, obtaining a pressure peak value in the injection pressure force curve; according to the trend of the injection pressure curve, operations such as line drawing and the like are performed to obtain a pressure peak value, and preferably, the pressure peak value is automatically judged and output through a computer, so that the obtained pressure peak value is accurate.
Step 28, the pressure peak value and the second comparison data are subjected to difference to obtain a third parameter; the third parameter is obtained by subtracting the pressure peak value obtained in step 27 from the second comparison data selected in step 22.
When the judgment result of the step 26 is that the liquid is normally injected, the method further comprises the following steps:
and 29, comparing the third parameter with a preset fifth threshold, judging that the sampling needle is in an abnormal liquid injection working state if the third parameter is smaller than the fifth threshold, and outputting an abnormal liquid injection signal. The working state is judged, and a proper fifth threshold value is preset according to the liquid characteristic difference of the sample, the liquid absorption amount and the like; and (3) comparing the third parameter calculated in the step (28) with a fifth threshold value to obtain the working state that the sampling needle is in abnormal liquid injection, namely, the situation that the needle is not completely blocked when the liquid is injected through the sample injection needle can be eliminated, and the accuracy of a detection result is improved.
On the basis of the above embodiment, the step S26 further includes the steps of:
step 261, when the second parameter is greater than the fourth threshold, judging that the sampling needle is in a working state of sample viscosity, and outputting a sample viscosity signal;
step S262, when the second parameter is smaller than the third threshold value, comparing the second comparison data with the preset range,
if the second comparison data is larger than the preset range, judging that the sampling needle is in a needle blocking working state, and outputting a needle blocking signal;
and if the second contrast data is smaller than or equal to the preset range, judging that the sampling needle is in the working state of empty injection, and outputting an empty injection signal.
Specifically, as shown in fig. 6, in step S261, the case where the second parameter is greater than the fourth threshold in step S26 is determined as a working state where the sample is viscous.
In step S262, on the premise that the second parameter in step S26 is smaller than the third threshold, a proper range is preset according to the difference of the liquid characteristics of the samples, the size of the liquid absorption amount, and the like, the second comparison data selected in step S22 is compared with the preset range, the working state of missed adding of the sampling needle is divided into the working states of sticky samples, needle blocking or empty injection, and corresponding sticky sample signals, needle blocking signals and empty injection signals are output outwards, so that operators or instruments can conveniently and specifically distinguish the cause of missed adding, proper countermeasures can be made, and the time and cost possibly consumed due to unclear distinguishing can be reduced.
On the basis of the above embodiment, when the result of step 26 and step 29 is determined to be the working state of the missed feeding or abnormal liquid injection, the method further includes the following steps:
step S30, the liquid suction is performed again to perform the refill, and the process returns to step S21. The operations of liquid suction and liquid injection are carried out again, detection is carried out during liquid injection, and the waste of the sample can be effectively avoided by supplementing the sample.
In addition to the above-mentioned method for detecting TIP loading anomalies based on pressure detection, the present invention also provides a detection device capable of executing the method for detecting TIP loading anomalies based on pressure detection disclosed in the above-mentioned embodiment, which comprises a sampling needle 1, a pressure sensor 2 and a controller. The pressure sensor 2 is used for collecting a pressure value inside the sampling needle 1 as comparison data when the sampling needle 1 does not work, and is used for sampling a real-time pressure value inside the sampling needle 1 when the sampling needle 1 works; the controller is used for comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle 1, wherein the working state comprises a normal state and an abnormal state, and outputs a signal of the working state.
The sampling needle 1, the pressure sensor 2 and the controller in the detection device are used for correspondingly executing each step in the TIP sample addition abnormality detection method based on pressure detection, and the controller is used for controlling the operation execution of the sampling needle 1 and the pressure sensor 2 and is a central control device. The content of the above method may be referred to the embodiment related to the detection device, and will not be described herein.
Specifically, as shown in fig. 10, the sampling needle 1 is used for making a sample, preferably, a TIP and various adaptive instruments are used, the disposable TIP can avoid carrying a pollution source, and the cost is reduced, particularly, the pressure value in the needle is collected as comparison data when the sampling needle 1 works each time by the pressure detection-based TIP sampling abnormality detection method, so that the sampling needle 1 is suitable for a TIP sampling system, and the simple TIP sampling system can be realized, and comprises operations of pushing a TIP to absorb liquid, injecting liquid and the like by a plunger pump.
The pressure sensor 2 is communicated with the inner cavity of the sampling needle 1 and is used for collecting the pressure value in the sampling needle 1; the controller and the pressure sensor 2 can perform signal transmission, and after receiving the pressure value acquired by the pressure sensor 2, the controller processes the data and outputs the judgment result, and it should be noted that the type of the controller, such as a computer, a circuit board, etc., is not limited, so long as the above functions can be realized.
The controller may control other sensors or execution means to execute the contents of the above-described method, so that the controller serves as an executor of the above-described method to achieve the above-described objects and effects.
In addition to the above-mentioned method for detecting the abnormal TIP loading based on pressure detection, the present invention also provides another detection device capable of executing the method for detecting the abnormal TIP loading based on pressure detection disclosed in the above-mentioned embodiment, where the detection device includes a controller and an actuator, the controller stores the above-mentioned method for detecting the abnormal TIP loading based on pressure detection, and the controller is connected to the actuator and is used for controlling the actuator to execute the steps of the method for detecting the abnormal TIP loading based on pressure detection.
Specifically, the actuator comprises a base, a sampling needle, a mechanism for clamping the sampling needle, a transmission mechanism, a sensor and the like, the controller is in signal connection with the actuator, and the controller stores the TIP sample addition abnormality detection method based on pressure detection.
When the device is used, under the action of the controller, the mechanism, the transmission mechanism and the like for clamping the sampling needle are controlled to drive the sampling needle to perform operations such as needle falling, liquid suction, liquid injection, needle lifting and the like, the sensor is controlled to acquire the pressure value in the sampling needle, and the controller operates each step of the TIP sample addition abnormality detection method based on the pressure detection according to the acquired data to obtain the working state of the sampling needle or further processes according to the working state obtained by judgment.
The specific type of the controller is not limited, and the TIP loading abnormality detection method based on pressure detection described above may be stored and executed.
The terms "upper surface, lower surface, top, bottom" and the terms "upper, lower, left and right" are defined based on the drawings of the specification.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The TIP sample addition abnormality detection method and the detection device based on the pressure detection provided by the invention are described in detail above. The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present invention and its core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the invention can be made without departing from the principles of the invention and these modifications and adaptations are intended to be within the scope of the invention as defined in the following claims.
Claims (10)
1. The TIP sample addition abnormality detection method based on pressure detection is characterized by comprising the following steps of:
collecting a pressure value in the needle as comparison data when the sampling needle does not work;
collecting a real-time pressure value in the sampling needle when the sampling needle works;
comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle, wherein the working state comprises a normal state and an abnormal state;
and outputting the signal of the working state.
2. The method for detecting a TIP loading abnormality based on pressure detection according to claim 1, comprising, when the sampling needle aspirates liquid:
collecting the pressure value in the sampling needle as first contrast data before each imbibition;
in the imbibition process, collecting the real-time pressure value and drawing into an imbibition pressure curve;
selecting a first data interval from the imbibition pressure curve;
taking absolute values of the real-time pressure values of the first data interval and the first comparison data respectively, and taking corresponding first absolute difference values;
summing the first absolute difference values to obtain a first parameter;
comparing the first parameter with a preset first threshold value and a preset second threshold value,
when the first parameter is smaller than the first threshold, judging that the sampling needle is in a suction working state, and outputting a signal for alarming suction;
when the first parameter is greater than or equal to the first threshold value and the first parameter is less than or equal to the second threshold value, judging that the sampling needle is in a working state of normal liquid suction, and outputting a signal of normal liquid suction;
when the first parameter is larger than the second threshold, judging that the sampling needle is in a working state of needle blocking, and outputting a signal for alarming the needle blocking.
3. The method for detecting abnormal TIP loading based on pressure detection according to claim 2, wherein the pipetting pressure curve is divided into a pipetting start section, a stationary pipetting section and a pipetting stop section, and the first data section is located in the stationary pipetting section.
4. The method for detecting abnormal TIP loading based on pressure detection according to claim 2, wherein when the judgment result is that the needle is blocked, further comprising:
sucking, beating and uniformly mixing the sample;
and controlling the sampling needle to suck liquid again, and collecting the real-time pressure value and drawing the real-time pressure value into a liquid sucking pressure curve in the liquid sucking process.
5. The method for detecting a TIP loading abnormality based on pressure detection according to any one of claims 1 to 4, comprising, when the sampling needle is filled:
in the liquid injection process, collecting the real-time pressure value and drawing to form an injection pressure force curve;
after each injection is completed, collecting the pressure value of the sampling needle after the internal pressure is stabilized as second comparison data;
selecting a section after the pressure peak value from the injection pressure force curve as a second data section;
taking absolute values of the real-time pressure values of the second data interval and the second comparison data respectively, and obtaining corresponding second absolute difference values;
summing the second absolute differences to obtain a second parameter;
comparing the second parameter with a preset third threshold value and a preset fourth threshold value,
when the second parameter is greater than or equal to the third threshold value and the second parameter is less than or equal to the fourth threshold value, judging that the sampling needle is in a working state of normal liquid injection, and outputting a signal of normal liquid injection;
and when the second parameter is smaller than the third threshold value or the second parameter is larger than the fourth threshold value, judging that the sampling needle is in a non-adding working state, and outputting a signal for alarming the non-adding.
6. The pressure detection-based TIP loading anomaly detection method of claim 5, wherein the pressure peak in the injection pressure curve is obtained;
the pressure peak value and the second comparison data are subjected to difference to obtain a third parameter;
when judging that the liquid is normally injected, comparing the third parameter with a preset fifth threshold value,
and if the third parameter is smaller than the fifth threshold, judging that the sampling needle is in an abnormal liquid injection working state, and outputting an abnormal liquid injection signal.
7. The method for detecting abnormal TIP loading based on pressure detection according to claim 5, wherein when the second parameter is greater than the fourth threshold, the sampling needle is judged to be in a working state in which the sample is viscous, and a sample viscous signal is output;
comparing the second comparison data with a preset range when the second parameter is smaller than the third threshold value,
if the second comparison data is larger than the preset range, judging that the sampling needle is in a needle blocking working state, and outputting a needle blocking signal;
and if the second comparison data is smaller than or equal to the preset range, judging that the sampling needle is in the working state of empty injection, and outputting an empty injection signal.
8. The method for detecting abnormal TIP loading based on pressure detection according to claim 6, wherein when the judgment result is a working state of missed loading or abnormal liquid injection, further comprising:
and (3) carrying out liquid suction again to carry out filling, and collecting the real-time pressure value and drawing the real-time pressure value into a liquid injection pressure curve in the liquid injection returning process.
9. A detection apparatus, characterized by comprising:
a sampling needle (1);
a pressure sensor (2) for acquiring a pressure value inside the sampling needle (1) as comparison data when the sampling needle (1) is not in operation, and for a real-time pressure value inside the sampling needle (1) when the sampling needle (1) is in operation;
and the controller is used for comparing the real-time pressure value with the comparison data to obtain the working state of the sampling needle (1), wherein the working state comprises a normal state and an abnormal state, and outputting a signal of the working state.
10. A detection device, characterized by comprising a controller and an actuator, wherein the controller stores the TIP loading abnormality detection method based on pressure detection according to any one of claims 1 to 8, and the controller is connected to the actuator for controlling the actuator to execute the step of the TIP loading abnormality detection method based on pressure detection.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116793574A (en) * | 2023-08-25 | 2023-09-22 | 新誉集团有限公司 | Fault detection method and system for train pressure wave sensor |
| CN117129136A (en) * | 2023-08-30 | 2023-11-28 | 安图实验仪器(郑州)有限公司 | Multi-channel pressure detection method, device and medium based on Tip sample addition |
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- 2023-01-17 CN CN202310084342.5A patent/CN116008577A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116793574A (en) * | 2023-08-25 | 2023-09-22 | 新誉集团有限公司 | Fault detection method and system for train pressure wave sensor |
| CN116793574B (en) * | 2023-08-25 | 2023-11-07 | 新誉集团有限公司 | Fault detection method and system for train pressure wave sensor |
| CN117129136A (en) * | 2023-08-30 | 2023-11-28 | 安图实验仪器(郑州)有限公司 | Multi-channel pressure detection method, device and medium based on Tip sample addition |
| CN117129136B (en) * | 2023-08-30 | 2024-09-06 | 安图实验仪器(郑州)有限公司 | Multi-channel pressure detection method, device and medium based on Tip sample addition |
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