CN116004375A - Device and method for detecting microorganism content in ocean food - Google Patents
Device and method for detecting microorganism content in ocean food Download PDFInfo
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- CN116004375A CN116004375A CN202310140118.3A CN202310140118A CN116004375A CN 116004375 A CN116004375 A CN 116004375A CN 202310140118 A CN202310140118 A CN 202310140118A CN 116004375 A CN116004375 A CN 116004375A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a device and a method for detecting the microorganism content in ocean foods, and belongs to the field of food detection. The detection device for the microbial content in the marine food comprises a detection box body, wherein a partition plate is fixedly connected inside the detection box body, and the detection box body is divided into a reaction cavity and a culture cavity through the partition plate; the invention is used for containing nutrient agar prepared in advance through the arrangement of the storage container, controlling the nutrient agar to accurately drop into the corresponding test tube through the arrangement of the drip nozzle and the valve, enabling the nutrient agar to react with the sample, controlling the storage container to move upwards after the sample is solidified through the mutual matching between the nutrient agar and the electric push rod, and enabling the test tube to move into the culture cavity for culture while avoiding the drip nozzle from influencing the movement of the test tube. The method has the technical effect of being capable of integrally detecting the microbial content in the marine food.
Description
Technical Field
The invention relates to the technical field of food detection, in particular to a device and a method for detecting the microorganism content in ocean foods.
Background
Because marine organisms have unique nutritional value and contain various bioactive substances, the marine organisms become an important source of human health-care foods and marine medicaments more and more, and development of the marine foods has become a development direction in the future, however, in the processing process of the marine foods, germs often enter the foods along with production of raw materials, packaging and product storage and transportation, so that food pollution is caused, and microorganisms in the foods need to be detected before selling.
The existing microorganism content detection method generally comprises the steps of manually taking a certain amount of food samples, mixing the samples with a proper amount of physiological saline, grinding the samples, mixing the samples, dripping nutrient agar prepared in advance, finally placing the nutrient agar into an incubator at thirty-seven ℃ for culturing forty-eight hours, and observing the total number of colonies on the nutrient agar to judge whether the microorganism content meets the standard.
The detection method needs to be provided with a plurality of instruments in a laboratory, lacks a set of integrated detection equipment, has more manual operation places, and can cause the phenomenon that different detection personnel are confused in instrument placement when in use.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, a plurality of instruments are needed to be arranged in a laboratory, a set of integrated detection equipment is lacked, and the number of manual operation places is large, so that the phenomenon that different detection personnel can cause the disordered placement of the instruments when in use is solved, and the detection device and the detection method for the microbial content in the marine food are provided.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a detection apparatus for microorganism content in ocean food, includes the detection box, the inside fixedly connected with baffle of detection box, separate into reaction chamber and cultivate the chamber through the baffle in the detection box, cultivate the inner wall fixed mounting in chamber and have the heater, the inlet port has been seted up at the middle part of baffle, the inside of reaction chamber is provided with the mounting panel, a side fixedly connected with cardboard that the mounting panel is close to the baffle, the cardboard can be embedded in the inlet port, a side fixedly connected with mounting box of cardboard, the spacing groove that is used for holding the test tube has been seted up to the middle part equidistance of mounting box, the reaction intracavity is provided with storage mechanism, there is the transport mechanism who is used for driving the test tube to get into to cultivate the intracavity through coupling assembling linkage on the storage mechanism, a side of cardboard is provided with the fixed establishment who is used for fixed test tube, the front of reaction chamber articulates through the hinge has the sliding door.
Preferably, the storage mechanism comprises an electric push rod fixedly connected to the top of the detection box body, a storage container is fixedly connected to the bottom end of the electric push rod extension rod, a drip nozzle is communicated with the bottom of the storage container at equal intervals, a valve is arranged on the outer surface of the drip nozzle, a liquid inlet pipe is communicated to the top of the storage container, the liquid inlet pipe is in sliding connection with the top of the detection box body, and a connecting rack is fixedly connected to one side surface of the storage container.
Preferably, the connecting assembly comprises a connecting gear meshed with the connecting rack, a rotating rod is fixedly connected to the axle center of the connecting gear, an L-shaped block is fixedly connected to the inner wall of the reaction cavity, the rotating rod is rotationally connected with the L-shaped block through a bearing, a main belt gear is fixedly connected to the outer surface of the rotating rod, a secondary belt gear is further arranged in the reaction cavity, and the main belt gear is connected with the secondary belt gear through a connecting belt in a transmission manner.
Preferably, the transportation mechanism comprises a screw rod fixedly connected to the axis of the secondary belt gear, two supporting blocks are fixedly connected to the inside of the reaction cavity, the screw rod is rotationally connected with the two supporting blocks through bearings, a moving block is connected to the outer surface of the screw rod in a threaded mode, a connecting piece is fixedly connected to the top of the moving block, the mounting plate is fixedly connected to one end of the connecting piece, two sliding rails are symmetrically and fixedly connected between the two supporting blocks, two limiting pieces are symmetrically and fixedly connected to the bottom of the moving block, and the two limiting pieces are respectively and slidably connected to the outer surfaces of the corresponding sliding rails.
Preferably, the fixed establishment is including fixing the groove piece in cardboard one side, the inside of groove piece is connected with the connecting rod through the bearing rotation, the surface fixedly connected with first circular gear of connecting rod, the surface meshing of first circular gear is connected with the second circular gear, the surface meshing of second circular gear is connected with the third circular gear, the second circular gear is all rotated with the groove piece through the pivot and is connected with the third circular gear, the equal fixedly connected with rubber stick of surface of second circular gear and third circular gear.
Preferably, the top end of the connecting rod is fixedly connected with a knob, and the outer surface of the knob is provided with an anti-skid groove.
Preferably, the outer surface of the clamping plate is fixedly connected with a sealing gasket.
Preferably, the position of the drip nozzle corresponds to the position of the limit groove.
A detection method of a detection device for the microorganism content in ocean foods comprises the following steps:
step A: sterilizing the test tube and the required vessel in an environment of one hundred twenty one degrees centigrade, preparing 3-4 parts of nutrient agar, placing the nutrient agar into a storage container through a liquid inlet pipe, taking 6-10 parts of physiological saline, placing the physiological saline into the test tube together, mixing the physiological saline with a sample of marine food, placing the test tube into different limiting grooves after fully and uniformly mixing, and fixing the test tube by using a fixing mechanism;
and (B) step (B): the valve is opened, so that nutrient agar in the storage container can be dripped into the test tube from the drip nozzle to react with a sample in the test tube, and the electric push rod is started and can drive the storage container to move upwards, so that the connecting racks move upwards together;
step C: when the connecting rack moves upwards, the connecting rack is meshed with the connecting gear, the connecting gear and the rotating rod can be driven to rotate, the rotating rod drives the main belt gear to rotate, when the main belt gear rotates, the secondary belt gear and the screw rod can be driven to rotate through the transmission effect of the connecting belt, as the screw rod is in threaded connection with the moving block and the limiting piece is in sliding connection with the sliding rail to limit, the moving block can be driven to move towards the direction of the culture cavity, the test tube moves together until the test tube moves into the culture cavity, the heater is started, the temperature in the culture cavity is thirty-seven ℃, the sample is cultured, after forty-eight hours of culture, the electric push rod is started again, the mounting box and the test tube are driven to reset, and the staff can judge whether the microbial content of the test tube accords with the standard or not through observing the quantity of the bacterial colony.
Compared with the prior art, the invention provides a device and a method for detecting the microorganism content in ocean foods, which have the following beneficial effects:
1. this detection device of microorganism content in ocean food is through storage container's setting for hold the nutrition agar that prepares in advance, and through the setting of nozzle and valve, be used for controlling nutrition agar accurate instil into corresponding test tube, make it produce the reaction with sample itself, through mutually supporting between the electric putter again, after the sample solidifies, be used for controlling storage container and upwards remove, when avoiding the nozzle to influence test tube removal, can also make the test tube remove to cultivate the intracavity, cultivate, through setting up the mounting panel, the cardboard, the mounting box, storage mechanism and cultivate the chamber in detecting the box, make the detection integration of sample, the instrument fixed point is placed, also can avoid appearing different inspectors when using, the chaotic phenomenon of instrument placement appears.
2. This detection device of microorganism content in ocean food, through connecting gear, the bull stick, L type piece, main belt gear, secondary belt gear, the setting of connecting belt, when electric putter drive storage container upwards moves, can also drive transport mechanism operation, this kind of design, the actuating force that makes electric putter produce can play different effects, make its rational utilization, the structure is ingenious, the linkage is strong, again through the lead screw, the supporting shoe, the movable block, the connecting piece, the slide rail, mutually support between the spacing piece, can be used to drive the mounting panel, the cardboard and the mounting box is moved towards the direction of cultivateing the chamber, make the test tube of placing the sample follow and remove together, until the cardboard is plugged up the inlet port, make the test tube remove to cultivate the intracavity, this kind of design, the manual operation degree of difficulty is less, and need not run back and forth to use before the different instruments, comparatively convenient and fast.
3. This detection device of microorganism content in ocean food, through the setting of groove block, connecting rod, first circular gear, second circular gear, third circular gear, pivot, rubber stick for fix the test tube, and can adjust according to the size of test tube, conveniently fix not unidimensional test tube, and spacing groove and fixed establishment set up to a plurality ofly, can once only detect the multiunit sample, through the contrast, make the data that detects more accurate.
The device has the advantages that the device is integrated with the detection of the sample by arranging the mounting plate, the clamping plate, the mounting box, the storage mechanism and the culture cavity in the detection box, the instrument is placed at fixed points, the phenomenon that different detection personnel are confused in instrument placement during use can be avoided, in addition, the manual operation difficulty is small, and the device does not need to run back and forth to be used in front of different instruments, so that the device is convenient and quick.
Drawings
Fig. 1 is a schematic diagram of a structure of a device for detecting microorganism content in marine food according to the present invention;
FIG. 2 is a schematic view of the internal structure of a reaction chamber of a device for detecting the microorganism content in a marine food according to the present invention;
FIG. 3 is a schematic sectional view of a device for detecting the microorganism content in a marine food according to the present invention;
FIG. 4 is a schematic diagram of a storage mechanism of a device for detecting the microorganism content in a marine food according to the present invention;
FIG. 5 is a schematic diagram of the structure of a connection assembly of a device for detecting the microorganism content in a marine food according to the present invention;
FIG. 6 is a schematic structural view of a mounting plate of a device for detecting the microorganism content in a marine food according to the present invention;
fig. 7 is a schematic structural diagram of a fixing mechanism of a device for detecting the microorganism content in the marine food.
In the figure: 1. detecting a box body; 2. a partition plate; 3. a reaction chamber; 4. a culture chamber; 5. a heater; 6. an access port; 7. a mounting plate; 8. a clamping plate; 9. a mounting box; 10. a limit groove; 1101. an electric push rod; 1102. a storage container; 1103. a drip nozzle; 1104. a valve; 1105. a liquid inlet pipe; 1106. the connecting rack; 12. a connection assembly; 1201. a connecting gear; 1202. a rotating rod; 1203. an L-shaped block; 1204. a main belt gear; 1205. a secondary belt gear; 1206. a connecting belt; 13. a transport mechanism; 1301. a screw rod; 1302. a support block; 1303. a moving block; 1304. a connecting piece; 1305. a slide rail; 1306. a limiting piece; 14. a fixing mechanism; 1401. a trough block; 1402. a connecting rod; 1403. a first circular gear; 1404. a second circular gear; 1405. a third circular gear; 1406. a rotating shaft; 1407. a rubber rod; 15. a sliding door; 16. a knob; 17. and a sealing gasket.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Example 1:
referring to fig. 1-7, a detection device for microorganism content in marine food comprises a detection box body 1, wherein a baffle plate 2 is fixedly connected to the inside of the detection box body 1, a reaction cavity 3 and a culture cavity 4 are formed in the detection box body 1 through the baffle plate 2, a heater 5 is fixedly arranged on the inner wall of the culture cavity 4, an inlet port 6 is formed in the middle of the baffle plate 2, a mounting plate 7 is arranged in the reaction cavity 3, a clamping plate 8 is fixedly connected to one side surface of the mounting plate 7 close to the baffle plate 2, the clamping plate 8 can be embedded in the inlet port 6, a mounting box 9 is fixedly connected to one side surface of the clamping plate 8, limit grooves 10 for containing test tubes are formed in the middle of the mounting box 9 at equal intervals, a storage mechanism is arranged in the reaction cavity 3, a conveying mechanism 13 for driving the test tubes to enter the culture cavity 4 is linked on the storage mechanism through a connecting component 12, a fixing mechanism 14 for fixing the test tubes is arranged on one side surface of the clamping plate 8, a sliding door 15 is hinged to the front surface of the reaction cavity 3 through a hinge, and a sealing gasket 17 is fixedly connected to the outer surface of the clamping plate 8.
In the invention, when detecting the microorganism content of ocean foods, a test tube and a required vessel are sterilized under the environment of one hundred twenty one degrees centigrade, then 3-4 parts of nutrient agar is prepared, and is put into a storage mechanism, a certain amount of different samples are respectively put into corresponding test tubes, the design can compare a plurality of groups of measured data, the accuracy of the detected data is enhanced, then 6-10 parts of physiological saline is put into the test tubes together, so that the physiological saline is mixed with the samples of the ocean foods, after the physiological saline is fully and uniformly mixed, the test tubes are put into different limiting grooves 10, the test tubes are fixed by utilizing a fixing mechanism 14, so that the test tubes are prevented from moving when nutrient agar is dripped into the corresponding test tubes, and the nutrient agar can be dripped into the corresponding test tubes through the storage mechanism, so that the nutrient agar reacts with the samples, after the sample is solidified, the storage mechanism is started again, the transportation mechanism 13 is driven to synchronously operate through the linkage effect of the connecting component 12, the transportation mechanism 13 is utilized to drive the mounting plate 7, the clamping plate 8 and the mounting box 9 to move towards the direction of the culture cavity 4, so that the test tube for placing the sample moves along until the clamping plate 8 blocks the inlet 6, the inlet 6 and the clamping plate 8 are sealed through the arrangement of the sealing gasket 17, the heat is prevented from flowing out, the detection precision is influenced, the test tube moves into the culture cavity 4, the heater 5 is started, the temperature in the culture cavity 4 is thirty-seven ℃, the sample is cultured, the storage mechanism is started again after forty-eight hours of culture, the transportation mechanism 13 is utilized to drive the mounting box 9 and the test tube to reset, the sliding door 15 is started, and the staff can judge whether the microbial content accords with the standard or not through observing the number of the bacterial colonies, the design is that the mounting plate 7, the clamping plate 8, the mounting box 9, the storage mechanism and the culture cavity 4 are all arranged in the detection box body 1, and the arrangement of the storage mechanism, the connecting component 12 and the conveying mechanism 13 is matched, so that the detection of the sample is integrated, the instrument is placed at fixed points, and the phenomenon that different detection personnel can place the instrument confusedly when in use can be avoided.
Example 2:
referring to fig. 1-7, a detection device for microorganism content in marine food comprises a detection box body 1, a baffle plate 2 is fixedly connected to the inside of the detection box body 1, a reaction cavity 3 and a culture cavity 4 are formed in the detection box body 1 through the baffle plate 2, a heater 5 is fixedly arranged on the inner wall of the culture cavity 4, an inlet 6 is formed in the middle of the baffle plate 2, a mounting plate 7 is arranged in the reaction cavity 3, a clamping plate 8 is fixedly connected to one side surface of the mounting plate 7 close to the baffle plate 2, the clamping plate 8 can be embedded in the inlet 6, a mounting box 9 is fixedly connected to one side surface of the clamping plate 8, a limit groove 10 for containing test tubes is formed in the middle of the mounting box 9 at equal intervals, a storage mechanism is arranged in the reaction cavity 3, a conveying mechanism 13 for driving the test tubes to enter the culture cavity 4 is arranged on the storage mechanism, a fixing mechanism 14 for fixing the test tubes is arranged on one side surface of the clamping plate 8, a front 1103 of the reaction cavity 3 is hinged with a sliding door 15 through a hinge, the storage mechanism comprises an electric push rod 1101 fixedly connected to the top of the detection box body 1, a storage container 1102 is fixedly connected to the bottom of the electric push rod, a storage container 1102 is fixedly connected to the bottom of the storage container 1102, a position of the storage container is connected to the corresponding top of the storage container 1102 is connected to the corresponding container 1105, and the top of the storage container is connected to the corresponding container 1105, and the storage container is connected to the top container is connected to the corresponding container 1105, and the storage container is connected to the storage container, and the storage container is connected to the storage container 1.
In the invention, when nutrient agar is put in, the nutrient agar flows into the storage container 1102 from the liquid inlet pipe 1105, the storage container 1102 can be made of heat-insulating materials for enhancing the detection precision, a detector can open the valve 1104 through the corresponding initial position of the drip nozzle 1103 and the limit groove 10, so that the nutrient agar in the storage container 1102 can drip into a test tube from the drip nozzle 1103 to react with a sample in the test tube, the valve 1104 can be closed after dripping, the electric push rod 1101 is started, the electric push rod 1101 can drive the storage container 1102 to move upwards, the connecting rack 1106 moves upwards along with the movement of the test tube, and the drip nozzle 1103 is prevented from blocking the movement of the test tube.
Example 3:
referring to fig. 1-7, a detection device for microorganism content in marine food comprises a detection box body 1, wherein a baffle plate 2 is fixedly connected in the detection box body 1, the detection box body 1 is internally divided into a reaction cavity 3 and a culture cavity 4 by the baffle plate 2, a heater 5 is fixedly arranged on the inner wall of the culture cavity 4, an inlet 6 is arranged in the middle of the baffle plate 2, a mounting plate 7 is arranged in the reaction cavity 3, a clamping plate 8 is fixedly connected with one side surface of the mounting plate 7 close to the baffle plate 2, the clamping plate 8 can be embedded in the inlet 6, a mounting box 9 is fixedly connected with one side surface of the clamping plate 8, a limit groove 10 for containing test tubes is formed in the middle of the mounting box 9 at equal intervals, a storage mechanism is arranged in the reaction cavity 3, a conveying mechanism 13 for driving the test tubes to enter the culture cavity 4 is linked on the storage mechanism by a connecting component 12, a fixing mechanism 14 for fixing the test tubes is arranged on one side surface of the clamping plate 8, the front of the reaction chamber 3 is hinged with a sliding door 15 through a hinge, the connecting component 12 comprises a connecting gear 1201 which is meshed with a connecting rack 1106, a rotating rod 1202 is fixedly connected at the axle center of the connecting gear 1201, the inner wall of the reaction chamber 3 is fixedly connected with an L-shaped block 1203, the rotating rod 1202 is rotationally connected with the L-shaped block 1203 through a bearing, the outer surface of the rotating rod 1202 is fixedly connected with a main belt gear 1204, a secondary belt gear 1205 is further arranged in the reaction chamber 3, the main belt gear 1204 is in transmission connection with the secondary belt gear 1205 through a connecting belt 1206, the conveying mechanism 13 comprises a screw rod 1301 which is fixedly connected at the axle center of the secondary belt gear 1205, two supporting blocks 1302 are fixedly connected inside the reaction chamber 3, the screw rod 1301 is rotationally connected with the two supporting blocks 1302 through a bearing, the outer surface of the screw rod 1301 is in threaded connection with a moving block 1303, the top of the moving block 1303 is fixedly connected with a connecting piece 1304, the mounting plate 7 is fixedly connected to one end of the connecting piece 1304, two sliding rails 1305 are symmetrically and fixedly connected between the two supporting blocks 1302, two limit pieces 1306 are symmetrically and fixedly connected to the bottom of the moving block 1303, and the two limit pieces 1306 are respectively and slidably connected to the outer surfaces of the corresponding sliding rails 1305.
In the invention, when the electric push rod 1101 drives the connecting rack 1106 to move upwards, the connecting rack 1106 is meshed with the connecting gear 1201, so that the connecting gear 1201 and the rotating rod 1202 can be driven to rotate, the rotating rod 1202 drives the main belt gear 1204 to rotate, the stability of the rotating rod 1202 can be enhanced through the arrangement of the L-shaped block 1203, when the main belt gear 1204 rotates, the secondary belt gear 1205 and the screw rod 1301 can be driven to rotate through the transmission effect of the connecting belt 1206, when the electric push rod 1101 drives the storage container 1102 to move upwards, the conveying mechanism 13 can be driven to synchronously operate while the drip nozzle 1103 is prevented from affecting the movement of a test tube, the lifting of the storage container 1102 and the operation of the conveying mechanism 13 are driven by one power supply of the electric push rod 1101, the number of power supplies is reduced, the independent installation of the power supply is not required to drive the conveying mechanism 13 to operate, the structure is ingenious, and when the screw rod 1301 rotates, the screw rod 1301 is in threaded connection with the moving block 1303 and the limit piece 1306 is in sliding connection with the slide rail to limit, so that the moving block 1303 can be driven to move along the direction of the culture cavity 4 until the test tube moves along with the culture cavity 4, the test tube, and the test tube moves to the test tube, and the test tube moves into the culture cavity 4, and the design is convenient to run to and around.
Example 4:
referring to figures 1-7, a detection device for microorganism content in marine food comprises a detection box body 1, wherein a baffle plate 2 is fixedly connected in the detection box body 1, a reaction cavity 3 and a culture cavity 4 are divided into the detection box body 1 through the baffle plate 2, a heater 5 is fixedly arranged on the inner wall of the culture cavity 4, an inlet 6 is arranged in the middle of the baffle plate 2, a mounting plate 7 is arranged in the reaction cavity 3, a clamping plate 8 is fixedly connected with one side surface of the mounting plate 7 close to the baffle plate 2, the clamping plate 8 can be embedded in the inlet 6, a mounting box 9 is fixedly connected with one side surface of the clamping plate 8, a limit groove 10 for accommodating test tubes is formed in the middle of the mounting box 9 at equal intervals, a storage mechanism is arranged in the reaction cavity 3, a conveying mechanism 13 for driving the test tubes to enter the culture cavity 4 is linked on the storage mechanism through a connecting component 12, A fixing mechanism 14 for fixing test tubes is arranged on one side face of the clamping plate 8, a sliding door 15 is hinged to the front face of the reaction cavity 3 through a hinge, the fixing mechanism 14 comprises a groove block 1401 fixed on one side face of the clamping plate 8, a connecting rod 1402 is connected to the inside of the groove block 1401 through bearing rotation, a first circular gear 1403 is fixedly connected to the outer surface of the connecting rod 1402, a second circular gear 1404 is connected to the outer surface of the first circular gear 1403 in a meshed mode, a third circular gear 1405 is connected to the outer surface of the second circular gear 1404 in a meshed mode, the second circular gear 1404 and the third circular gear 1405 are connected to the groove block 1401 in a rotating mode through a rotating shaft 1406, rubber rods 1407 are fixedly connected to the outer surfaces of the second circular gear 1404 and the third circular gear 1405, a knob 16 is fixedly connected to the top end of the connecting rod 1402, and an anti-skid groove is formed in the outer surface of the knob 16.
In the invention, when a test tube is required to be fixed, the test tube is placed in the limit groove 10, a detection person turns the knob 16, the knob 16 drives the connecting rod 1402 to rotate, the arrangement of the knob 16 is used for enabling the rotation of the connecting rod 1402 to be more convenient, the connecting rod 1402 drives the first circular gear 1403 to rotate, the first circular gear 1403 is meshed with the second circular gear 1404 to drive the second circular gear 1404 to rotate, the second circular gear 1404 drives the third circular gear 1405 to rotate, and as the rotation directions of the second circular gear 1404 and the third circular gear 1405 are opposite, the two rubber rods 1407 can be driven to rotate towards the middle, the test tube is fixed by the two rubber rods 1407, the test tube is prevented from being broken due to overlarge pressure, the positions of the two rubber rods 1407 can be changed, and the test tube can be adjusted according to the size of the test tube, so that the test tube with different sizes can be conveniently fixed.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (9)
1. The utility model provides a detection device of microorganism content in ocean food, includes detection box (1), its characterized in that, the inside fixedly connected with baffle (2) of detection box (1), reaction chamber (3) and cultivate chamber (4) are separated into through baffle (2) in detection box (1), the inner wall fixed mounting of cultivateing chamber (4) has heater (5), inlet port (6) have been seted up at the middle part of baffle (2), the inside of reaction chamber (3) is provided with mounting panel (7), a side fixedly connected with cardboard (8) that mounting panel (7) are close to baffle (2), cardboard (8) can imbed in inlet port (6), a side fixedly connected with mounting box (9) of cardboard (8), spacing groove (10) that are used for holding the test tube have been seted up to the middle part equidistance of mounting box (9), be provided with storage mechanism in reaction chamber (3), there are transport mechanism (13) that are used for driving the test tube to get into in cultivateing chamber (4) through coupling assembling (12), a side (8) are provided with hinge (14) fixed through hinge (15) in front fixed connection door (15).
2. The detection device for the microbial content in the marine food according to claim 1, wherein the storage mechanism comprises an electric push rod (1101) fixedly connected to the top of the detection box body (1), a storage container (1102) is fixedly connected to the bottom end of an extension rod of the electric push rod (1101), a drip nozzle (1103) is communicated with the bottom of the storage container (1102) at equal intervals, a valve (1104) is arranged on the outer surface of the drip nozzle (1103), a liquid inlet pipe (1105) is communicated to the top of the storage container (1102), the liquid inlet pipe (1105) is in sliding connection with the top of the detection box body (1), and a connecting rack (1106) is fixedly connected to one side surface of the storage container (1102).
3. The device for detecting the microbial content in the marine food according to claim 2, wherein the connecting assembly (12) comprises a connecting gear (1201) which is meshed with the connecting rack (1106), a rotating rod (1202) is fixedly connected to the axle center of the connecting gear (1201), an L-shaped block (1203) is fixedly connected to the inner wall of the reaction cavity (3), the rotating rod (1202) is rotatably connected with the L-shaped block (1203) through a bearing, a main belt gear (1204) is fixedly connected to the outer surface of the rotating rod (1202), a secondary belt gear (1205) is further arranged in the reaction cavity (3), and the main belt gear (1204) is in transmission connection with the secondary belt gear (1205) through a connecting belt (1206).
4. The detection device for the microbial content in the marine food according to claim 3, wherein the transportation mechanism (13) comprises a screw rod (1301) fixedly connected to the axis of the secondary belt gear (1205), two supporting blocks (1302) are fixedly connected to the inside of the reaction chamber (3), the screw rod (1301) is rotatably connected with the two supporting blocks (1302) through a bearing, a moving block (1303) is connected to the outer surface of the screw rod (1301) in a threaded manner, a connecting piece (1304) is fixedly connected to the top of the moving block (1303), two sliding rails (1305) are symmetrically and fixedly connected to one end of the connecting piece (1304) between the two supporting blocks (1302), two limiting pieces (1306) are symmetrically and fixedly connected to the bottom of the moving block (1303), and the two limiting pieces (1306) are respectively and slidably connected to the outer surfaces of the corresponding sliding rails (1305).
5. The device for detecting the microbial content in the marine food according to claim 1, wherein the fixing mechanism (14) comprises a groove block (1401) fixed on one side surface of the clamping plate (8), a connecting rod (1402) is rotatably connected to the inside of the groove block (1401) through a bearing, a first circular gear (1403) is fixedly connected to the outer surface of the connecting rod (1402), a second circular gear (1404) is connected to the outer surface of the first circular gear (1403) in a meshed manner, a third circular gear (1405) is connected to the outer surface of the second circular gear (1404) in a meshed manner, the second circular gear (1404) and the third circular gear (1405) are rotatably connected with the groove block (1401) through rotating shafts (1406), and rubber rods (1407) are fixedly connected to the outer surfaces of the second circular gear (1404) and the third circular gear (1405).
6. The device for detecting the microbial content in the marine food according to claim 5, wherein the top end of the connecting rod (1402) is fixedly connected with a knob (16), and an anti-skid groove is formed on the outer surface of the knob (16).
7. The device for detecting the microbial content in the marine food according to claim 1, wherein the outer surface of the clamping plate (8) is fixedly connected with a sealing gasket (17).
8. The device for detecting the microbial content in the marine food according to claim 2, wherein the drip nozzle (1103) corresponds to the position of the limit groove (10).
9. A detection method using the detection device for the microbial content in a marine food according to claim 4, comprising the steps of:
step A: sterilizing the test tube and the required vessel in an environment of one hundred twenty one degrees centigrade, preparing 3-4 parts of nutrient agar, placing the nutrient agar into a storage container (1102) through a liquid inlet pipe (1105), taking 6-10 parts of physiological saline, placing the physiological saline into the test tube together, mixing the physiological saline with a sample of ocean foods, placing the test tube into different limiting grooves (10) after fully and uniformly mixing, and fixing the test tube by using a fixing mechanism (14);
and (B) step (B): opening a valve (1104) to enable nutrient agar in a storage container (1102) to drop into a test tube from a drop nozzle (1103) to react with a sample in the test tube, and starting an electric push rod (1101), wherein the electric push rod (1101) can drive the storage container (1102) to move upwards so as to enable a connecting rack (1106) to move upwards along with the storage container;
step C: when the connecting rack (1106) moves upwards, the connecting rack (1106) is meshed with the connecting gear (1201), so that the connecting gear (1201) and the rotating rod (1202) can be driven to rotate, the rotating rod (1202) drives the main belt gear (1204) to rotate, when the main belt gear (1204) rotates, the secondary belt gear (1205) and the screw rod (1301) can be driven to rotate through the transmission effect of the connecting belt (1206), the screw rod (1301) is in threaded connection with the moving block (1303) and the limiting piece (1306) is in sliding connection with the sliding rail (1305) to limit the sliding way, the moving block (1303) can be driven to move towards the direction of the culture cavity (4), the test tube moves along until the test tube moves into the culture cavity (4), the heater (5) is started, the temperature in the culture cavity (4) is enabled to be at thirty-seven ℃, the sample is cultured, after forty-eight hours of culture, the electric push rod (1101) is started again, the mounting box (9) and reset can be driven, and a worker can observe the quantity of the colony to judge whether the microorganism content accords with the standard.
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