CN115999193A - Multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and use method - Google Patents
Multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and use method Download PDFInfo
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Abstract
The invention provides a multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and a use method thereof; the X-axis linear motion unit and the X-axis linear motion unit are arranged on the experimental platform, a liquid transferring pump is fixed on the X-axis linear motion unit, and the two groups of liquid transferring needle assemblies are communicated through the liquid transferring pump; the X-axis linear motion unit is simultaneously provided with a liquid level monitoring vision camera component driven by the Z-axis linear motion unit; the experiment platform is provided with a pipetting needle cleaning unit and two rows of extraction bottle racks, and each row of bottle racks can be used for placing a plurality of groups of extraction bottles; the visual liquid level monitor is used for detecting whether the liquid level of the liquid-liquid extraction separation liquid is generated or not and monitoring the liquid level; the cleaning unit is used for cleaning the pipetting needle body; the experimental platform is also provided with a code reader. According to the invention, the pretreatment and liquid-liquid extraction tasks of a plurality of samples are realized through automatic control of multiple stations, the pipetting needle body is cleaned through the cleaning unit, so that reagent pollution is avoided, the automatic switching of multiple tasks is realized, the efficiency is improved, and errors are reduced.
Description
Technical Field
The invention belongs to the field of laboratory automatic extraction equipment, and particularly relates to a multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and a use method thereof.
Background
Liquid-liquid extraction is based on the separation and concentration of the target substance by the difference in partition coefficients of the solutes in two mutually immiscible liquids. The extraction phase is to be separated, and the solvent after extraction is called as raffinate phase. Liquid-liquid extraction is one of the common pretreatment methods for experimental and analytical samples. The extraction test has the advantages of complicated operation, strong repeatability, long duration and continuity requirement. At present, most of the complex test operations are manually completed, the damage to human bodies is large, the efficiency is low, and the misoperation rate of long-time test is also increased.
Disclosure of Invention
In view of the above, the invention aims to provide a multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and a use method thereof, and the pretreatment of a plurality of samples is realized by automatically controlling the multi-station, so that the efficiency is improved, and the errors are reduced.
In order to achieve the above purpose, the technical scheme of the invention is realized as follows:
the multi-station continuous full-automatic liquid-liquid extraction device based on AI vision liquid level monitoring comprises an X-axis linear motion unit arranged on an experimental platform, a liquid transfer pump, a liquid level monitoring vision camera component, two groups of liquid transfer needle components, an extraction bottle rack, a cleaning unit and a computer central control system;
the two groups of extraction bottle racks are arranged on the experimental platform in parallel, the bottom of each group of extraction bottle racks is provided with a magnetic stirrer, the extraction bottle racks are used for placing extraction bottles, and the magnetic stirrers are used for rotating the liquid in the extraction bottles to realize full mixing of the liquid;
the X-axis linear motion unit is arranged between the two groups of extraction bottle racks, and is also provided with a Z-axis linear motion unit, so that the Z-axis linear motion unit can move in the X-axis direction;
the Z-axis linear motion unit is provided with a liquid transfer pump and two groups of liquid transfer needle assemblies, and each group of liquid transfer needle assemblies corresponds to one Z-axis linear athlete; the two groups of pipetting needle assemblies can move simultaneously or singly in the Z-axis direction; the two groups of pipetting needle assemblies are communicated with each other and are respectively communicated with the pipetting pump; the two groups of pipetting needle assemblies are in one-to-one correspondence with the two groups of extraction bottle racks;
the end part of each group of extraction bottle racks is provided with a cleaning unit which is used for cleaning the inner wall and the outer wall of the pipetting needle body of the pipetting needle assembly;
the X-axis linear motion unit is also provided with a liquid level monitoring visual camera component which is used for monitoring whether the liquid level of the liquid-liquid extraction in the extraction bottle is generated or not;
the experiment platform is also provided with a code reader; the X-axis linear motion unit, the Z-axis linear motion unit, the liquid transfer pump, the liquid level monitoring vision camera component, the magnetic stirrer and the cleaning unit are all connected to the computer central control system.
Further, the position of each magnetic stirrer is used as a working position, and the position information of each working position is stored in a computer central control system.
Further, the two pipetting needle bodies of the two pipetting needle assemblies have moving tracks corresponding to the two groups of extraction bottle racks respectively.
Further, the pipetting needle body is used for sucking liquid in the extraction bottle or inputting the liquid into the extraction bottle.
Further, the cleaning unit comprises a cleaning pipe and a liquid discharge pipeline, the cleaning pipe is arranged in the liquid discharge pipeline, the bottom of the cleaning pipe is connected to the cleaning liquid containing container through a first pipeline, and the bottom of the liquid discharge pipeline is communicated to the waste liquid discharge system through a second pipeline.
Further, the pipe diameter of the liquid discharge pipeline is larger than that of the cleaning pipe, and the pipe diameter of the cleaning pipe is larger than that of the pipetting needle body.
Furthermore, a cleaning pump and an electromagnetic valve are arranged in the first pipeline, and the cleaning pump and the electromagnetic valve are connected to a computer central control system through signals.
The application method of the multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring is characterized by comprising the following steps of:
the extraction liquid and the mixed liquid are in the same extraction bottle;
s11, determining components; placing the extraction bottle containing the liquid to be extracted into a row of extraction bottle racks with magnetic stirrers, and placing a new empty bottle with the same specification at a corresponding position in the other row of extraction bottle racks; before the extraction bottle is put into the extraction bottle rack, the bottle is held until a code reader reads the identity code of the bottle;
s12, magnetically stirring; controlling the magnetic stirrer to work, setting the revolution and stirring time of the magnetic stirrer through a computer central control system, controlling the magnetic stirrer to be closed through the computer central control system after stirring is finished,
s13, layering; standing for layering, and waiting for extraction;
s14, analyzing the liquid level; the extraction bottle after standing sends a notification instruction to a computer central control system, the computer central control system controls a visual camera and a pipetting unit to move to the position through an X-axis linear movement unit, the visual camera scans, photographs and stands the extraction bottle from top to bottom under the driving of the X-axis linear movement unit, and the liquid level dividing position is determined by an image algorithm;
s15, extracting and pipetting; the first pipetting needle of the Z-axis linear movement unit controller automatically moves downwards; according to the actual condition that liquid to be extracted is on the layered upper layer or lower layer, a first-number liquid-moving needle moves downwards to the top of a liquid separating surface or the bottom of a bottle, a second-number liquid-moving needle simultaneously moves to an empty bottle on the other side, a liquid-moving pump is controlled to work, liquid in the extraction bottle is moved to the empty bottle by liquid-moving operation, the position of the second-number liquid-moving needle in the empty bottle is set through a computer central control system, during liquid-moving, the liquid level of the second-number extraction bottle is detected through a visual liquid level monitor, and when the liquid level reaches the needle point position of the second-number liquid-moving needle, liquid-moving is stopped;
s16, cleaning a pipetting needle; the extraction bottle is internally provided with extraction remainder, a first pipetting needle and a second pipetting needle which finish pipetting operation enter a cleaning unit for cleaning under the cooperation of an X-axis linear movement unit and a Z-axis linear movement unit, and the next extraction is waited for after the cleaning is finished;
s17, collecting an extraction bottle; taking the second extraction bottle after the extraction is completed by a mechanical arm; the above-mentioned cyclic operation is continued until all extractions are completed.
The extraction liquid and the mixed liquid are respectively arranged in two extraction bottles;
s21, performing S21; placing in separate bottles; the first extraction bottle is filled with the extract liquid, the second extraction bottle is filled with the mixed liquid,
s22, performing S22; pipetting; the computer central control system controls the second moving frame to move the first pipetting needle into the first extraction bottle, the second pipetting needle into the second extraction bottle, controls the pipetting pump to work, adds the extract in the first extraction bottle into the mixed solution in the second extraction bottle,
s23, layering; after the first-size pipetting needle and the second-size pipetting needle are lifted, a computer central control system controls a magnetic stirrer to work to shake the second-size extraction bottle, layering is accelerated, and shaking time is set;
s24, detecting the liquid level; the first movable frame controls the visual liquid level monitor to move to the position of the second extraction bottle, detects layering condition of the liquid level, if the layering detection is qualified, the extraction is finished, if the layering detection is unqualified, the operation is continued until the extraction is finished;
s25, cleaning a pipetting needle; the second moving frame of the pipetting needle after the extraction is driven to enter a cleaning unit for cleaning, and the next extraction is waited after the cleaning is finished; and taking the second extraction bottle after the extraction is completed by a mechanical arm.
Compared with the prior art, the multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and the use method thereof have the following advantages:
(1) The invention relates to a multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and a use method thereof.
(2) According to the multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and the using method, the automatic control moving frame is adopted, the visual liquid level detector monitors in real time, the extraction of the extraction bottle is finished, the extraction bottle is automatically carried by a manipulator, the labor is saved, and the quality of the extraction is higher.
(3) The multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and the using method thereof, disclosed by the invention, realize a series of complex test flows of multi-task continuous full-automatic liquid-liquid extraction, wherein the automatic liquid-liquid transfer of a liquid transfer pump and the automatic needle washing and pipeline cleaning are realized; compared with the manual extraction, the intelligent full-automatic unmanned experimental process can be comprehensively realized, the automatic unmanned experimental process has high efficiency and low misoperation rate, so that the system can continuously execute multiple extraction tasks without pollution, and liquid-liquid extraction is realized efficiently and automatically.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. In the drawings:
fig. 1 is a schematic diagram of a multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring and a using method thereof according to an embodiment of the invention.
Reference numerals illustrate:
1. an experiment platform; 2. an X-axis linear movement unit; 3. an extraction bottle; 4. a transfer pump; 5. a liquid level monitoring vision camera assembly; 6. a pipetting needle assembly; 7. an extraction bottle rack; 8. a cleaning unit; 81. cleaning the tube; 82. a liquid discharge pipeline; 9. and a computer central control system.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art in a specific case.
The invention will be described in detail below with reference to the drawings in connection with embodiments.
The multi-station continuous full-automatic liquid-liquid extraction device based on AI vision liquid level monitoring is shown in fig. 1, and comprises an X-axis linear motion unit arranged on an experiment platform 1, a liquid transfer pump 4, a liquid level monitoring vision camera assembly 5, two groups of liquid transfer needle assemblies 6, an extraction bottle 3 frame, a cleaning unit 8 and a computer central control system 9;
the two groups of extraction bottle 3 frames are arranged on the experiment platform 1 in parallel, the bottom of each group of extraction bottle 3 frames is provided with a magnetic stirrer, the extraction bottle 3 frames are used for placing the extraction bottles 3, and the magnetic stirrers are used for rotating the liquid in the extraction bottles 3 to realize full mixing of the liquid;
in the embodiment 1, the bottoms of the two groups of extraction bottle racks are respectively provided with a magnetic stirrer, and in the embodiment 2, only one group of extraction bottle racks is provided with a magnetic stirrer;
the X-axis linear motion unit is arranged between the two groups of extraction bottle 3 frames, and the X-axis linear motion unit is also provided with a Z-axis linear motion unit, so that the Z-axis linear motion unit can move in the X-axis direction. The X-axis linear motion unit and the Z-axis linear motion unit can respectively realize a module capable of linearly moving, and are parts which can be purchased in the market;
the Z-axis linear motion unit is provided with a liquid transfer pump 4 and two groups of liquid transfer needle assemblies 6, and each group of liquid transfer needle assemblies 6 corresponds to one Z-axis linear motion unit; realizing simultaneous movement or single movement of the two groups of pipetting needle assemblies 6 in the Z-axis direction; the two groups of pipetting needle assemblies 6 are communicated with each other, exchange liquid between the pipetting needle assemblies and the pipetting needle assemblies through a movable pump, and are respectively communicated to a liquid source through a pipetting pump 4 for sucking the extraction liquid; the two groups of pipetting needle assemblies 6 are in one-to-one correspondence with the two groups of extraction bottle 3 frames;
the end part of each group of extraction bottle 3 frames is provided with a cleaning unit 8, and the cleaning unit 8 is used for cleaning the inner wall and the outer wall of the pipetting needle body of the pipetting needle assembly 6;
the X-axis linear motion unit is also provided with a liquid level monitoring visual camera component 5 for monitoring whether the liquid level of the liquid-liquid extraction in the extraction bottle 3 is generated or not;
the experiment platform 1 is also provided with a code reader; the X-axis linear motion unit, the Z-axis linear motion unit, the liquid transfer pump 4, the liquid level monitoring vision camera assembly 5, the magnetic stirrer and the cleaning unit 8 are all connected to a computer central control system 9.
Preferably, the position of each magnetic stirrer is taken as a station, and the position information of each station is stored in the computer central control system 9.
As example 1, the layout of the magnetic stirrers is disordered, and before use, the position information of the magnetic stirrers is recorded into the computer central control system 9, and the computer central control system 9 controls the pipetting needle body to the target setting position;
as example 2, the magnetic stirrers in the extraction zone are distributed in groups, at least two magnetic stirrers are grouped as extraction groups, and the extraction flask 3 corresponds to the magnetic stirrers one by one, for example: the two magnetic stirrers are taken as a group, and the magnetic stirrers are arranged in a total of 4 groups, namely, the arrangement of the magnetic stirrers is distributed in two rows, and each row is 4; specifically, the magnetic stirrer is commercially available equipment, and the liquid in the extraction flask 3 is shaken by the magnetic stirrer to accelerate the stratification.
Preferably, the two pipetting needle bodies of the two pipetting needle assemblies 6 have movement tracks corresponding to the two groups of extraction bottle 3 frames respectively.
As an embodiment, the number of the pipetting needle bodies is two or more, the two or more pipetting needle bodies are communicated to the pipetting pump 4 through a pipeline, the pipetting pump 4 is communicated to other liquid sources through a pipeline, the two or more pipetting needle bodies can be corresponding to one liquid source, possibly a plurality of pipetting needle bodies can also be corresponding to one liquid source, and the two or more pipetting needle bodies can be communicated through the pipetting pump 4, namely, an a pipetting needle body and a B pipetting needle body, under the operation of the pipetting pump 4, the a pipetting needle body can suck the C liquid, and then the C liquid is output to a target position through the B pipetting needle body;
the liquid transfer pump 4 is a product which can be purchased in the market, and preferably, the liquid can directly circulate in a pipeline without passing through the liquid transfer pump 4, so that the pollution to the liquid after passing through the liquid transfer pump 4 is avoided, and the inaccuracy of experimental data is caused.
Preferably, the pipetting needle body is used for sucking up the liquid in the extraction bottle 3 or inputting the liquid into the extraction bottle 3.
Preferably, the cleaning unit 8 comprises a cleaning pipe 81 and a liquid discharge pipeline 82, the cleaning pipe 81 is arranged in the liquid discharge pipeline 82, the bottom of the cleaning pipe 81 is connected to the cleaning liquid containing container through a first pipeline, and the bottom of the liquid discharge pipeline 82 is communicated to the waste liquid discharge system through a second pipeline; preferably, a cleaning pump and an electromagnetic valve are arranged in the first pipeline, and the cleaning pump and the electromagnetic valve are connected to the computer central control system 9 in a signal mode.
The computer central control system 9 controls the cleaning pump and the electromagnetic valve to be opened, clean liquid passes through the first pipeline runner cleaning pipe 81 from a liquid source, the water for cleaning continuously flows out in a one-way, the water flows out from the cleaning pipe 81 to enter the liquid discharge pipeline 82 and then is discharged, the environment of the platform is not polluted and destroyed,
preferably, the pipe diameter of the liquid discharge pipeline 82 is larger than that of the cleaning pipe 81, and the pipe diameter of the cleaning pipe 81 is larger than that of the pipetting needle body; the positions of the liquid discharge pipeline 82 and the cleaning pipe 81 are fixed, and position information is input into the computer central control system 9, so that the pipetting needle body can be conveniently and rapidly and accurately introduced into the cleaning pipe 81 under the drive of the second mobile stage frame under the control of the computer central control system 9;
preferably, the computer central control system 9 is a PLC or other electronic control units, and the mature computer central control system 9 is used for controlling the whole extraction process, so that the real objects are reduced, and the efficiency is improved.
Before each extraction process starts, the mechanical arm grabs the extraction bottles 3 and moves to a code reader device, the bottle body of each extraction bottle 3 is provided with an identity information two-dimensional code, the code reader reads the identity of the two-dimensional code and then transmits a signal to a computer central control system 9, and the computer central control system 9 recognizes and then places the extraction bottle 3 in a target position; after the program, the corresponding relation between the identification number of the extraction bottle 36 and the liquid data is uploaded to the computer central control system 9, and the recording and tracking of the data identity are completed;
the application method of the multi-station continuous full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring comprises the following steps:
the example 1, the extract and the mixture are in the same extraction flask 3;
s11, determining components; placing the extraction bottle 3 containing the liquid to be extracted into a row of extraction bottle 3 frames with a magnetic stirrer, and placing a new empty bottle with the same specification at a corresponding position in the other row of extraction bottle 3 frames; the full-automatic liquid-liquid extraction system is characterized in that the loading and unloading of various bottles are completed by receiving a central control instruction by a manipulator; an independent liquid-liquid extraction station, wherein the putting in and taking out of various bottles are completed by personnel; whether a full-automatic system or an independent workstation is adopted, before the extraction bottle 3 is put into the extraction bottle 3 frame before being put into various bottles of a liquid-liquid extraction workstation, the bottle identity code is read by a bottle holding-to-code reader; the identity on the bottle body can be a test bar code, a two-dimensional code, an RFID code and the like;
s12, magnetically stirring; the magnetic stirrer is controlled to work, the revolution number and the stirring time of the magnetic stirrer are set through the computer central control system 9, the magnetic stirrer is controlled to be closed by the computer central control system 9 after the stirring is finished,
s13, layering; standing for layering, and waiting for extraction;
s14, analyzing the liquid level; the extraction bottle 3 which is placed still sends a notification instruction to the computer central control system 9, the computer central control system 9 controls the vision camera and the pipetting unit to move to the position through the X-axis linear movement unit 2, the vision camera scans, photographs and places the extraction bottle 3 from top to bottom under the driving of the X-axis linear movement unit 2, and the liquid dividing level position is determined by an image algorithm;
s15, extracting and pipetting; the first pipetting needle of the Z-axis linear movement unit controller automatically moves downwards; according to the actual condition that liquid to be extracted is on the layered upper layer or lower layer, a first-number liquid-moving needle moves downwards to the top of a liquid separating surface or the bottom of a bottle, a second-number liquid-moving needle simultaneously moves to an empty bottle on the other side, a liquid-moving pump 4 is controlled to work, liquid in an extraction bottle 3 is moved to the empty bottle by liquid-moving operation, the position of the second-number liquid-moving needle in the empty bottle is set through a computer central control system 9, during liquid-moving, the liquid level of the second-number extraction bottle 3 is detected through a visual liquid level monitor, and when the liquid level reaches the needle point position of the second-number liquid-moving needle, liquid-moving is stopped;
s16, cleaning a pipetting needle; the extraction bottle 3 is internally provided with extraction remainder, a first pipetting needle and a second pipetting needle which finish pipetting operation enter a cleaning unit 8 for cleaning under the cooperation of an X-axis linear movement unit 2 and a Z-axis linear movement unit, and the next extraction is waited for after the cleaning is finished;
s17, collecting the extract in the extraction bottle 3; taking out the second extraction bottle 3 after extraction is completed by a mechanical arm; the above-mentioned cyclic operation is continued until all extractions are completed.
Example 2, extract and mixed liquor were in two extraction bottles 3, respectively;
s21, performing S21; placing in separate bottles; the first extraction bottle 3 is internally provided with an extraction liquid, the second extraction bottle 3 is internally provided with a mixed liquid,
s22, performing S22; pipetting; the computer central control system 9 controls the second moving frame to move the first pipetting needle into the first extraction bottle 3, the second pipetting needle into the second extraction bottle 3, controls the pipetting pump 4 to work, adds the extraction liquid in the first extraction bottle 3 into the mixed liquid in the second extraction bottle 3,
s23, layering; after the first pipetting needle and the second pipetting needle are lifted, the computer central control system 9 controls the magnetic stirrer to work to shake the second extraction bottle 3, accelerate layering and set shaking time;
s24, detecting the liquid level; the first movable frame controls the visual liquid level monitor to move to the position of the second extraction bottle 3, detects layering condition of the liquid level, if the layering detection is qualified, the extraction is finished, if the layering detection is unqualified, the operation is continued until the extraction is finished;
s25, cleaning a pipetting needle; the second moving frame of the pipetting needle after extraction enters a cleaning unit 8 to be cleaned under the driving of the second moving frame of the pipetting needle after the extraction is finished, and the next extraction is waited to be carried out after the cleaning is finished; and taking out the second extraction bottle 3 after the extraction is completed by a mechanical arm.
In embodiment 3, the cleaning unit 8, the pipetting needle body and the pipetting pump 4 are matched to clean the pipetting needle body and the pipeline, so that the device can continuously perform extraction tasks without pollution.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (8)
1. Multistation continuous full-automatic liquid-liquid extraction device based on AI vision liquid level monitoring, its characterized in that: the device comprises an X-axis linear motion unit, a liquid transfer pump, a liquid level monitoring vision camera assembly, two groups of liquid transfer needle assemblies, an extraction bottle rack, a cleaning unit and a computer central control system, wherein the X-axis linear motion unit is arranged on an experimental platform;
the two groups of extraction bottle racks are arranged on the experimental platform in parallel, the bottom of each group of extraction bottle racks is provided with a magnetic stirrer, the extraction bottle racks are used for placing extraction bottles, and the magnetic stirrers are used for rotating the liquid in the extraction bottles to realize full mixing of the liquid;
the X-axis linear motion unit is arranged between the two groups of extraction bottle racks, and is also provided with a Z-axis linear motion unit, so that the Z-axis linear motion unit can move in the X-axis direction;
the Z-axis linear motion unit is provided with a liquid transfer pump and two groups of liquid transfer needle assemblies, and each group of liquid transfer needle assemblies corresponds to one Z-axis linear athlete; the two groups of pipetting needle assemblies can move simultaneously or singly in the Z-axis direction; the two groups of pipetting needle assemblies are communicated with each other and are respectively communicated with the pipetting pump; the two groups of pipetting needle assemblies are in one-to-one correspondence with the two groups of extraction bottle racks;
the end part of each group of extraction bottle racks is provided with a cleaning unit which is used for cleaning the inner wall and the outer wall of the pipetting needle body of the pipetting needle assembly;
the X-axis linear motion unit is also provided with a liquid level monitoring visual camera component which is used for monitoring whether the liquid level of the liquid-liquid extraction in the extraction bottle is generated or not;
the experiment platform is also provided with a code reader; the X-axis linear motion unit, the Z-axis linear motion unit, the liquid transfer pump, the liquid level monitoring vision camera component, the magnetic stirrer and the cleaning unit are all connected to the computer central control system.
2. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device according to claim 1, wherein: the position of each magnetic stirrer is used as a working position, and the position information of each working position is stored in a computer central control system.
3. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device according to claim 1, wherein: the two pipetting needle bodies of the two pipetting needle assemblies have moving tracks corresponding to the two groups of extraction bottle racks respectively.
4. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device according to claim 2, wherein: the liquid-transferring needle body is used for sucking liquid in the extraction bottle or inputting the liquid into the extraction bottle.
5. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device according to claim 1, wherein: the cleaning unit comprises a cleaning pipe and a liquid discharge pipeline, the cleaning pipe is arranged in the liquid discharge pipeline, the bottom of the cleaning pipe is connected to the cleaning liquid containing container through a first pipeline, and the bottom of the liquid discharge pipeline is communicated to the waste liquid discharge system through a second pipeline.
6. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device as claimed in claim 5, wherein: the pipe diameter of the liquid discharge pipeline is larger than that of the cleaning pipe, and the pipe diameter of the cleaning pipe is larger than that of the pipetting needle body.
7. The AI-vision-liquid-level-monitoring-based multi-station continuous full-automatic liquid-liquid extraction device as claimed in claim 5, wherein: the first pipeline is provided with a cleaning pump and an electromagnetic valve which are connected to a computer central control system through signals.
8. The method for using the multi-station continuous and full-automatic liquid-liquid extraction device based on AI visual liquid level monitoring as claimed in any one of claims 1 to 7, comprising the following steps:
the extraction liquid and the mixed liquid are in the same extraction bottle;
s11, determining components; placing the extraction bottle containing the liquid to be extracted into a row of extraction bottle racks with magnetic stirrers, and placing a new empty bottle with the same specification at a corresponding position in the other row of extraction bottle racks; before the extraction bottle is put into the extraction bottle rack, the bottle is held until a code reader reads the identity code of the bottle;
s12, magnetically stirring; controlling the magnetic stirrer to work, setting the revolution and stirring time of the magnetic stirrer through a computer central control system, controlling the magnetic stirrer to be closed through the computer central control system after stirring is finished,
s13, layering; standing for layering, and waiting for extraction;
s14, analyzing the liquid level; the extraction bottle after standing sends a notification instruction to a computer central control system, the computer central control system controls a visual camera and a pipetting unit to move to the position through an X-axis linear movement unit, the visual camera scans, photographs and stands the extraction bottle from top to bottom under the driving of the X-axis linear movement unit, and the liquid level dividing position is determined by an image algorithm;
s15, extracting and pipetting; the first pipetting needle of the Z-axis linear movement unit controller automatically moves downwards; according to the actual condition that liquid to be extracted is on the layered upper layer or lower layer, a first-number liquid-moving needle moves downwards to the top of a liquid separating surface or the bottom of a bottle, a second-number liquid-moving needle simultaneously moves to an empty bottle on the other side, a liquid-moving pump is controlled to work, liquid in the extraction bottle is moved to the empty bottle by liquid-moving operation, the position of the second-number liquid-moving needle in the empty bottle is set through a computer central control system, during liquid-moving, the liquid level of the second-number extraction bottle is detected through a visual liquid level monitor, and when the liquid level reaches the needle point position of the second-number liquid-moving needle, liquid-moving is stopped;
s16, cleaning a pipetting needle; the extraction bottle is internally provided with extraction remainder, a first pipetting needle and a second pipetting needle which finish pipetting operation enter a cleaning unit for cleaning under the cooperation of an X-axis linear movement unit and a Z-axis linear movement unit, and the next extraction is waited for after the cleaning is finished;
s17, collecting an extraction bottle; taking the second extraction bottle after the extraction is completed by a mechanical arm; continuing the circulation operation until all the extraction is completed;
the extraction liquid and the mixed liquid are respectively arranged in two extraction bottles;
s21, performing S21; placing in separate bottles; the first extraction bottle is filled with the extract liquid, the second extraction bottle is filled with the mixed liquid,
s22, performing S22; pipetting; the computer central control system controls the second moving frame to move the first pipetting needle into the first extraction bottle, the second pipetting needle into the second extraction bottle, controls the pipetting pump to work, adds the extract in the first extraction bottle into the mixed solution in the second extraction bottle,
s23, layering; after the first-size pipetting needle and the second-size pipetting needle are lifted, a computer central control system controls a magnetic stirrer to work to shake the second-size extraction bottle, layering is accelerated, and shaking time is set;
s24, detecting the liquid level; the first movable frame controls the visual liquid level monitor to move to the position of the second extraction bottle, detects layering condition of the liquid level, if the layering detection is qualified, the extraction is finished, if the layering detection is unqualified, the operation is continued until the extraction is finished;
s25, cleaning a pipetting needle; the second moving frame of the pipetting needle after the extraction is driven to enter a cleaning unit for cleaning, and the next extraction is waited after the cleaning is finished; and taking the second extraction bottle after the extraction is completed by a mechanical arm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116359523A (en) * | 2023-05-30 | 2023-06-30 | 深圳市第二人民医院(深圳市转化医学研究院) | Medical automatic sample split charging instrument and system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116359523A (en) * | 2023-05-30 | 2023-06-30 | 深圳市第二人民医院(深圳市转化医学研究院) | Medical automatic sample split charging instrument and system |
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