CN115990526A - Microfluidic experimental platform with high integration level - Google Patents
Microfluidic experimental platform with high integration level Download PDFInfo
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- CN115990526A CN115990526A CN202310288138.5A CN202310288138A CN115990526A CN 115990526 A CN115990526 A CN 115990526A CN 202310288138 A CN202310288138 A CN 202310288138A CN 115990526 A CN115990526 A CN 115990526A
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Abstract
The invention discloses a high-integration microfluidic experimental platform, which relates to the technical field of microfluidics, and comprises a main board box and a cover body movably connected to one side edge of the main board box, wherein an upper computer module is integrally fixed on the inner surface of the cover body; the fluid control module comprises a plurality of pump bodies, valve bodies, detection components and control components which are integrally arranged on the top surface of the main board box; the temperature control module and the photoelectric detection module are integrally arranged on the top surface of the main board box. The upper computer module of the invention communicates and controls the sub-components of the fluid driving module, the temperature control module and the photoelectric detection module; the fluid control module is used for fluid driving, fluid switching and flow detection functions; the temperature control module is used for adjusting the temperature rise and the temperature reduction in the chip; the photoelectric detection module is used for chip imaging and fluorescence detection; the invention has the advantages of high integration level, comprehensive microfluidic experiment function, simple and efficient operation, low cost, capability of meeting the requirements of various microfluidic experiments, and the like.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a high-integration microfluidic experimental platform.
Background
The microfluidic technology attracts attention of various universities, scientific research institutions and research and development institutions with the advantages of high integration, miniaturization, high flux, low reagent/sample consumption and no cross contamination. However, in practical use, the fluid control system, the detection system and the temperature control system involved in the microfluidic chip technology are all independent and not shared.
The invention patent application of application number 201310462255.5 discloses an experimental method and a microfluidic experimental device for multi-field filter membrane loss, wherein a reaction chamber, a liquid injection micro-channel and a filtrate micro-channel are arranged in a microfluidic chip, an ultrafiltration membrane component is fixedly arranged at the joint of the reaction chamber and the liquid injection micro-channel, and a PC (personal computer) is respectively connected with an electric field signal generator, a positive pressure pump, a photoelectric detection device, an ultrasonic signal generator and two back pressure pumps. In the prior art, the experimental system temporarily built according to the experimental requirements integrates independent system structures to form an integral new structure, but the mode cannot meet the requirements of various microfluidic experiments.
It can be seen that most of the microfluidic application devices are designed for a single application and cannot meet the use situations of various microfluidic chips in the same laboratory. All kinds of equipment need to be adapted before different experiments, the complex and low efficiency are caused, and the arrangement of all parts is messy, so that the space is occupied.
Therefore, how to provide a microfluidic experimental platform with high integration level, which can meet the use situations of various microfluidic chips in the same laboratory, is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the present invention provides a microfluidic experimental platform with high integration, which aims to solve the above technical problems.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a microfluidic experimental platform with high integration level comprises a main board box and a cover body movably connected to one side edge of the main board box; further comprises:
the upper computer module is integrally fixed on the inner surface of the cover body;
the fluid control module comprises a plurality of pump bodies, valve bodies, detection components and control components which are integrally arranged on the top surface of the main board box;
the temperature control module is integrally arranged on the top surface of the main board box and used for controlling the temperature of the chip;
the photoelectric detection module is integrally arranged on the top surface of the main board box and positioned below the temperature control module, and is used for chip imaging and fluorescence detection.
Through the technical scheme, the microfluidic experimental platform with high integration level comprises a main board box, an upper computer module, a fluid control module, a temperature control module and a photoelectric detection module, wherein the upper computer module is used for communicating and controlling all sub-components of the fluid driving module, the temperature control module and the photoelectric detection module; the fluid control module is used for fluid driving, fluid switching and flow detection functions; the temperature control module is used for adjusting the temperature rise and the temperature reduction in the chip; the photoelectric detection module is used for chip imaging and fluorescence detection; the invention has the advantages of high integration level, comprehensive microfluidic experiment function, simple and efficient operation, low cost, capability of meeting the requirements of various microfluidic experiments, and the like.
Preferably, in the above-mentioned high-integration microfluidic experimental platform, the top surface of the main board box has the mounting port of the fluid control module, the temperature control module and the photoelectric detection module, and a power supply and a circuit system electrically connected to each module are integrated inside the main board box. The power supply and the circuit system are integrated inside the main board box, and the mounting port is formed in the top surface of the main board box, so that the fluid control module, the temperature control module and the photoelectric detection module can be quickly connected through circuits.
Preferably, in the microfluidic experimental platform with high integration, the fluid control module includes an injection pump, a pneumatic controller, a peristaltic pump, a plunger pump, an electromagnetic valve, a switching valve, a flow detector and a liquid storage rack. Through the setting of above structure, can satisfy different experimental demands.
Preferably, in the microfluidic experimental platform with high integration, the fluid interfaces of the injection pump, the air pressure controller, the peristaltic pump and the plunger pump are all located on the surface of the main board box portion exposed out of the fluid interfaces, and the power supply and communication control interfaces are all located on the bottom surface of the main board box portion and are connected with the circuit system at the bottom or inside the main board box. Through the arrangement, the fluid connector of the pump body can be quickly connected with the micro-fluidic chip, and the components and the internal circuit of the main board box can be quickly and conveniently connected.
Preferably, in the above-mentioned high-integration microfluidic experimental platform, the temperature control module includes a mounting frame, a temperature control plate, a radiator, and a temperature sensor; the mounting frame is mounted on the top surface of the main board box, the temperature control plate is fixed on the mounting frame, the temperature control plate controls the temperature through a temperature control circuit system, and a temperature adjusting knob is mounted on the mounting frame; the radiator is installed on the mounting frame, the temperature sensor is installed on the mounting frame and used for detecting the temperature of the temperature control plate. The temperature control module can realize the lifting control of temperature, and realize fast adjustment through the knob that adjusts the temperature, utilizes the radiator heat dissipation simultaneously, prevents the influence of high temperature to other components and parts.
In order to meet the temperature regulation requirement, the temperature control module is further provided with a display screen connected with the temperature sensor so as to display the temperature conveniently.
Preferably, in the above-mentioned high-integration microfluidic experimental platform, the temperature control plate is ITO heat conducting glass, a metal plate, a metal mesh or peltier. The temperature control plates are all made of heat transfer medium materials, and can meet the requirement of rapid temperature transfer of the microfluidic chip.
Preferably, in the microfluidic experimental platform with high integration, the photoelectric detection module is located at the bottom of the mounting frame, and the photoelectric detection module includes a three-axis driving system, and a detection light source, a light path system and a photoelectric detector mounted on the three-axis driving system. The detection light source, the light path system and the photoelectric detector can meet the illumination and imaging acquisition requirements of experiments.
Preferably, in the above-mentioned high-integration microfluidic experimental platform, the detection light source is a halogen light source, an LED light source or a fluorescent light source; the light path system is an inverted imaging system, an inverted fluorescence detection system, a normal imaging system or a normal fluorescence detection system; the photodetector is CCD, CMOS, PMT or a high-speed CCD. Through the adaptive selection and collocation of the equipment, different experimental operations can be satisfied.
Preferably, in the above-mentioned high-integration microfluidic experimental platform, the main board box and the cover body are connected by a hinge structure, and are connected between the main board box and the cover body by a soft material, and the soft material is used for packaging the circuit. The main board box and the cover body can be movably connected and buckled, and the connection of the circuit can not be influenced.
Preferably, in the microfluidic experimental platform with high integration, soft cushions or rubber rings are embedded around or at four corners of the inner surface of the cover body. On the one hand, the sealing effect can be improved, and meanwhile, the collision damage can be prevented.
Compared with the prior art, the microfluidic experimental platform with high integration level has the following beneficial effects:
1. the high-integration microfluidic experimental platform equipment integrates various fluid control, fluid detection, temperature control and photoelectric equipment commonly used in microfluidic experiments, has complete functions and high integration and automation degree, and can realize most types of microfluidic experiments and researches.
2. According to the microfluidic experimental platform equipment with high integration, through reasonable space layout, the fluid pipeline is ensured to sequentially take over and operate a liquid storage bottle, a fluid control equipment, a fluid detection equipment and a photoelectric detection equipment in space in the use process, so that the space utilization rate is improved as much as possible while the winding of the pipeline is avoided.
3. According to the microfluidic experimental platform equipment with high integration, through the scheme of the upper computer opening and closing design, the lateral space is not influenced in the use process, and meanwhile, the microfluidic experimental platform equipment is directly closed and placed when the use is stopped, and the next use is not influenced by disassembling pipelines.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural diagram of a high-integration microfluidic experimental platform provided by the invention;
FIG. 2 is a schematic diagram showing a top view of the microfluidic experimental platform with high integration provided by the invention;
fig. 3 is a schematic structural diagram of a photodetection module according to the present invention.
Wherein:
1-a main board box; 2-a cover; 3-an upper computer module; 4-a fluid control module; 41-syringe pump; 42-air pressure controller; 43-peristaltic pump; 44-plunger pump; 45-electromagnetic valve; 46-a switching valve; 47-flow meter; 48-a liquid storage rack; 5-a temperature control module; 51-mounting rack; 52-a temperature control plate; 53-a temperature adjustment knob; 6-a photoelectric detection module; 61-triaxial drive system.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 3, the embodiment of the invention discloses a microfluidic experimental platform with high integration level, which comprises a main board box 1 and a cover body 2 movably connected with one side edge of the main board box 1; further comprises:
the upper computer module 3 is integrally fixed on the inner surface of the cover body 2;
the fluid control module 4, the fluid control module 4 comprises a plurality of pump bodies, valve bodies, detection components and regulation components which are integrally arranged on the top surface of the main board box 1;
the temperature control module 5 is integrally arranged on the top surface of the main board box 1 and is used for controlling the temperature of the chip;
the photoelectric detection module 6 is integrally arranged on the top surface of the main board box 1 and is positioned below the temperature control module 5, and the photoelectric detection module 6 is used for chip imaging and fluorescence detection.
In order to further optimize the technical scheme, the top surface of the main board box 1 is provided with the mounting ports of the fluid control module 4, the temperature control module 5 and the photoelectric detection module 6, and a power supply and a circuit system which are electrically connected with the modules are integrated in the main board box 1.
To further optimize the above technical solution, the fluid control module 4 comprises a syringe pump 41, a pneumatic controller 42, a peristaltic pump 43, a plunger pump 44, a solenoid valve 45, a switching valve 46, a flow meter 47 and a reservoir 48; the number of each sub-module can be 1-6.
The arrangement of the components on the surface of the main board box 1 in this embodiment needs to ensure a principle: the large module is arranged at four corners of the device, and the small module is arranged at the gap of the device. Wherein the integrated equipment of the large-sized temperature control module 5 and the photoelectric detection module 6 is arranged at the left and right outer sides below the surface of the main board box 1. The syringe pump 41, the air pressure controller 42, and the plunger pump 44 are disposed outside the other three corners of the surface of the main plate tank 1, i.e., the upper left, the upper right, and the left right. Smaller subsystems such as peristaltic pump 43, solenoid valve 45, switching valve 46, flow meter 47, etc. are placed in the device intermediate space. The liquid storage frame 48 is closely arranged on the left side or the right side of the peristaltic pump 43, the air pressure controller 42 and the photoelectric detection module 6, so that the use is convenient.
In order to further optimize the above technical solution, the injection pump 41, the air pressure controller 42, the peristaltic pump 43 and the plunger pump 44 may be disposed horizontally or vertically, the fluid interfaces are all located on the surface of the portion of the main board box 1 exposed, and the power supply and communication control interfaces are all located on the bottom surface of the main board box 1 and connected with the circuit system at the bottom or inside the main board box 1 by means of the fluid interfaces such as threads, luer, reverse taper and the like and the pipeline connection.
In order to further optimize the above technical solution, the temperature control module 5 comprises a mounting frame 51, a temperature control plate 52, a radiator and a temperature sensor; the mounting frame 51 is mounted on the top surface of the main board box 1, the temperature control plate 52 is fixed on the mounting frame 51, the temperature control plate 52 controls the temperature through a temperature control circuit system, and the temperature adjusting knob 53 is mounted on the mounting frame 51; the radiator is mounted on the mounting frame 51, and the temperature sensor is mounted on the mounting frame 51 and is used for detecting the temperature of the temperature control plate 52.
To further optimize the above solution, the temperature control plate 52 is ITO heat conducting glass, a metal plate, a metal mesh or peltier.
In order to further optimize the technical solution, the photo-detection module 6 is located at the bottom of the mounting frame 51, and the photo-detection module 6 includes a three-axis driving system 61, and a detection light source, a light path system and a photo-detector mounted on the three-axis driving system 61. In this embodiment, the three-axis driving system 61 is a conventional three-axis driving structure driven by a motor screw, which is a prior art and will not be described herein.
In order to further optimize the technical scheme, the detection light source is a halogen light source, an LED light source or a fluorescent light source; the light path system is an inverted imaging system, an inverted fluorescence detection system, a normal imaging system or a normal fluorescence detection system; the photodetector is CCD, CMOS, PMT or a high-speed CCD.
In order to further optimize the technical scheme, the main board box 1 and the cover body 2 are connected through a hinge structure, and particularly can be connected through mechanical pulleys, gears, elastic materials and the like; and is connected between the main board box 1 and the cover body 2 by a soft material for encapsulating the wiring.
In order to further optimize the technical scheme, soft cushions or rubber rings are embedded around or at four corners of the inner surface of the cover body 2.
In order to further optimize the above technical solution, the controllers required by the fluid control module 4, the temperature control module 5, the photoelectric detection module 6 and the sub-modules thereof may be packaged inside each module, and directly communicate with the upper computer module 3 for control. Or integrated on the same PCB control board, packaged in the cover body 2, and then communicated with the upper computer module 3 for control.
Example 1:
in this embodiment: the main board box 1 is designed to be opened and closed integrally, the cover body 2 is connected with the main board box 1 through a mechanical pulley, and flexible materials are adopted for packaging the travelling wires. The periphery of the inner surface of the cover body 2 is protected by a soft cushion.
The sub-modules of the fluid control module 4 include 4 syringe pumps 41, 4 air pressure controllers 42, 2 peristaltic pumps 43, 2 plunger pumps 44, 8 solenoid valves 45, 1 8-way switching valves 56, 4 flow detectors 47, and 4 reservoirs 48.
The temperature control module 5 comprises a temperature control plate 51, a radiator and a temperature sensor, wherein the temperature control plate 51 is ITO heat-conducting glass.
The photodetection module 6 comprises a detection light source, a light path system, a photodetector and a triaxial displacement system 61. The detection light source is an LED light source, the light path system is an inverted imaging system, and the photoelectric detector is a high-speed CCD.
The temperature control module 5 and the photoelectric detection module 6 are integrated, wherein the microfluidic chip is arranged above the temperature control plate 51 of the temperature control module 5.
The arrangement of the components on the surface of the main board box 1 is shown in fig. 1, that is, the large-scale integrated equipment of the temperature control module 5 and the photoelectric detection module 6 is arranged on the left outer side below the surface of the main board box 1. The syringe pump 41 is placed in the upper right corner, the air pressure controller 42 is placed in the lower right corner, and the plunger pump 44 is placed in the upper left corner of the surface of the main plate case 1. Smaller subsystems such as peristaltic pump 43, solenoid valve 45, switching valve 46, flow meter 47, etc. are placed in the device intermediate space.
The injection pump 41, the air pressure controller 42, the peristaltic pump 43 and the plunger pump 44 in the fluid control module 4 are transversely arranged, wherein a fluid interface is connected with a pipeline through an M6 threaded interface, and a power supply and communication control interface is arranged inside the main board box 1.
The controllers required by the fluid control module 4, the temperature control module 5, the photoelectric detection module 6 and the sub-modules thereof are packaged inside the modules and directly communicated with the upper computer module 3 for control.
The device can realize droplet generation and image acquisition, namely, the injection pump 41 is used for controlling the fluid movement and migration of organic phase and aqueous phase reagents, the switching valve 46 and the flow detector 47 are used for controlling the fluid flow rate and the channel direction, the inverted imaging system and the high-speed CCD are used for capturing droplet generation experiments, and the peristaltic pump 43 and the plunger pump 44 are used for cleaning the inside of the chip.
Example 2:
in this embodiment: the main board box 1 is designed to be opened and closed integrally, the cover body 2 is connected with the main board box 1 through elastic materials, and flexible materials are adopted for packaging the travelling wires. Four corners of the inner surface of the cover body 2 are protected by rubber rings.
The sub-modules of the fluid control module 4 include 4 syringe pumps 41, 2 air pressure controllers 42, 4 peristaltic pumps 43, 2 plunger pumps 44, 4 solenoid valves 45, 1 8- way switching valves 46, 4 flow detectors 47, and 4 reservoirs 48.
The temperature control module 5 comprises a temperature control plate 51, a radiator and a temperature sensor, wherein the temperature control plate 51 is a copper heat conducting plate.
The photodetection module 6 comprises a detection light source, a light path system, a photodetector and a triaxial displacement system 61. The detection light source is a fluorescent light source, the light path system is a forward fluorescent detection system, and the photoelectric detector is a CCD.
The temperature control module 5 and the photoelectric detection module 6 are integrated, wherein the microfluidic chip is arranged above the temperature control plate 51 of the temperature control module 5.
The arrangement mode of all the components on the surface of the main board box 1 is as follows: the integrated equipment of the temperature control module 5 and the photoelectric detection module 6 is arranged on the outer side of the right lower side of the surface of the main board box 1. The syringe pump 41 is placed in the upper right corner, the air pressure controller 42 is placed in the upper left corner, and the plunger pump 44 is placed in the lower left corner of the surface of the main tank 1. Smaller subsystems such as peristaltic pump 43, solenoid valve 45, switching valve 46, flow meter 47, etc. are placed in the device intermediate space.
The injection pump 41, the air pressure controller 42, the peristaltic pump 43 and the plunger pump 44 in the fluid control module 4 are vertically arranged, wherein a fluid interface is connected with a pipeline through a luer interface, and a power supply and communication control interface is arranged at the bottom of the module.
The controllers required by the fluid control module 4, the temperature control module 5, the photoelectric detection module 6 and the sub-modules thereof are integrated on the same PCB board, and are packaged inside the main board box 1 to be in communication control with the upper computer module 3.
Cell culture and observation on a multichannel organ chip can be realized through the equipment configuration, the carbon dioxide content in the chip is controlled through a pneumatic controller 42, the injection of an original gel cell embryo sample is controlled through a syringe pump 41, the reciprocating circulation of a culture solution is controlled through a plunger pump 44 and a peristaltic pump 43, and the cell growth condition is observed through a fluorescence imaging system and a CCD.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant points refer to the description of the method section.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The microfluidic experimental platform with high integration level comprises a main board box (1) and a cover body (2) movably connected to one side edge of the main board box (1); characterized by further comprising:
the upper computer module (3) is integrally fixed on the inner surface of the cover body (2);
the fluid control module (4), the fluid control module (4) comprises a plurality of pump bodies, valve bodies, detection components and regulation components which are integrally arranged on the top surface of the main board box (1);
the temperature control module (5) is integrally arranged on the top surface of the main board box (1) and used for controlling the temperature of the chip;
the photoelectric detection module (6), the photoelectric detection module (6) is integrated to be installed mainboard case (1) top surface, and is located temperature control module (5) below, photoelectric detection module (6) are used for chip formation of image and fluorescence detection.
2. The high-integration microfluidic experimental platform according to claim 1, wherein the top surface of the main board box (1) is provided with the fluid control module (4), the temperature control module (5) and the installation port of the photoelectric detection module (6), and a power supply and a circuit system electrically connected with each module are integrated inside the main board box (1).
3. The high-integration microfluidic experimental platform according to claim 2, wherein the fluid control module (4) comprises an injection pump (41), a pneumatic controller (42), a peristaltic pump (43), a plunger pump (44), a solenoid valve (45), a switching valve (46), a flow meter (47) and a liquid storage rack (48).
4. A microfluidic experimental platform with high integration according to claim 3, wherein the fluid interfaces of the injection pump (41), the air pressure controller (42), the peristaltic pump (43) and the plunger pump (44) are all located on the surface of the main board box (1) exposed, and the power supply and communication control interfaces are all located on the bottom surface and connected with the circuit system at the bottom or inside of the main board box (1).
5. The high-integration microfluidic experimental platform according to claim 1, wherein the temperature control module (5) comprises a mounting frame (51), a temperature control plate (52), a radiator and a temperature sensor; the mounting frame (51) is mounted on the top surface of the main board box (1), the temperature control plate (52) is fixed on the mounting frame (51), the temperature control plate (52) controls the temperature through a temperature control circuit system, and a temperature adjusting knob (53) is mounted on the mounting frame (51); the radiator is mounted on the mounting frame (51), and the temperature sensor is mounted on the mounting frame (51) and used for detecting the temperature of the temperature control plate (52).
6. The high-integration microfluidic experimental platform according to claim 5, wherein the temperature control plate (52) is ITO heat conducting glass, a metal plate, a metal mesh or peltier.
7. The high-integration microfluidic experimental platform according to claim 5 or 6, wherein the photoelectric detection module (6) is located at the bottom of the mounting frame (51), and the photoelectric detection module (6) comprises a three-axis driving system (61), and a detection light source, a light path system and a photoelectric detector which are installed on the three-axis driving system (61).
8. The high-integration microfluidic experimental platform according to claim 7, wherein the detection light source is a halogen light source, an LED light source or a fluorescent light source; the light path system is an inverted imaging system, an inverted fluorescence detection system, a normal imaging system or a normal fluorescence detection system; the photodetector is CCD, CMOS, PMT or a high-speed CCD.
9. The high-integration microfluidic experimental platform according to claim 1, wherein the main board box (1) and the cover body (2) are connected through a hinge structure, and are connected between the main board box (1) and the cover body (2) through a soft material, and the soft material is used for packaging a circuit.
10. The high-integration microfluidic experimental platform according to claim 1, wherein soft cushions or rubber rings are embedded around or at four corners of the inner surface of the cover body (2).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310288138.5A CN115990526A (en) | 2023-03-23 | 2023-03-23 | Microfluidic experimental platform with high integration level |
| CN202322142347.7U CN220573512U (en) | 2023-03-23 | 2023-08-10 | Microfluidic experimental platform with high integration level |
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| CN202310288138.5A CN115990526A (en) | 2023-03-23 | 2023-03-23 | Microfluidic experimental platform with high integration level |
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| CN202322142347.7U Active CN220573512U (en) | 2023-03-23 | 2023-08-10 | Microfluidic experimental platform with high integration level |
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Citations (10)
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