CN115960696B

CN115960696B - Biological reactor

Info

Publication number: CN115960696B
Application number: CN202211605319.8A
Authority: CN
Inventors: 杨梅; 柯文婷; 陈咏梅; 郭胜娟
Original assignee: Wuhan City Vocational College
Current assignee: Wuhan City Vocational College
Priority date: 2022-12-14
Filing date: 2022-12-14
Publication date: 2023-08-04
Anticipated expiration: 2042-12-14
Also published as: CN115960696A

Abstract

The invention provides a bioreactor, which comprises a bioreactor body, wherein the bioreactor body comprises a base, a barrel body is fixed on the base, a sealing disc is slidably arranged between the barrel body and the base, a fixed seat is welded at the top of the barrel body, a movable seat is movably arranged at the top of the fixed seat, the movable seat is connected with the fixed seat through a fixing mechanism, a lifting mechanism is fixed on the movable seat, an air inlet pipe is arranged at the side of the lifting mechanism, an exhaust pipe is arranged at the side of the air inlet pipe, and the exhaust pipe and the air inlet pipe are both fixed on the movable seat. This bioreactor, during the use, the inside toper seat of stirring subassembly cooperatees through inlet opening and wash port and stirs living beings, and the position of toper seat can be adjusted from top to bottom during the stirring, accomplishes stirring to the living beings in different positions and uses when adjusting from top to bottom, gets into living beings through the inlet opening and discharges from the wash port again, and living beings bump in inside and are favorable to stirring and use.

Description

Biological reactor

Technical Field

The invention relates to the field of bioreactor equipment, in particular to a bioreactor.

Background

When the organisms are cultured, the microorganisms are required to be cultured through the bioreactor, however, when the temperature control is realized, the common bioreactor in the market at present usually heats a heating medium such as water through a heat source, and then heats a culture solution in the reactor through the heated heating medium, but when the culture solution reaches a set temperature, a large amount of heat still exists in the heating medium, the temperature of the culture solution is continuously increased, the temperature change is continuously caused, the temperature control precision of the culture solution is lower, and the temperature control of the culture solution is unfavorable. In addition, the stirring mechanism inside the existing bioreactor can only realize the stirring function of organisms, and can not enable the temperature inside to be rapidly cooled when the organisms need to be cooled.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a bioreactor for solving the problems in the background art, the invention has novel structure, when the bioreactor is used, a conical seat in a stirring assembly is matched with a water inlet hole and a water outlet hole to stir organisms, the position of the conical seat can be adjusted up and down during stirring, and the organisms at different positions can be stirred and used during up and down adjustment, so that stirring efficiency is improved.

In order to achieve the above object, the present invention is realized by the following technical scheme: the utility model provides a bioreactor, includes the bioreactor body, the bioreactor body includes the base, be fixed with the staving on the base, slidable mounting has the sealing disk between staving and the base, the top welding of staving has the fixing base, the top movable mounting of fixing base has movable seat, be connected through fixed establishment between movable seat and the fixing base, be fixed with elevating system on the movable seat, the intake pipe is installed to elevating system's avris, the blast pipe is installed to the avris of intake pipe, blast pipe and intake pipe are all fixed on the movable seat, the bottom of base is fixed with motor case and bracing piece.

The motor box is internally provided with a power mechanism, the power mechanism is fixed at the bottom of the base, an output shaft is arranged on the power mechanism, and a stirring assembly is arranged on the output shaft.

The inside of staving is fixed with the inner bag, stirring subassembly is installed the inside of inner bag, the inside of inner bag contains heating mechanism, connecting assembly is installed at stirring subassembly's top.

The movable column is fixed on the connecting component, the heating plate is installed at the bottom of the movable column, one end of the movable column is installed on the lifting mechanism, and the drainage component is installed between the liner and the barrel body.

The drainage assembly comprises a support tube, one end of the support tube is arranged on the liner, a movable tube is slidably arranged in the support tube, and a connecting flange is welded at one end of the movable tube.

The stirring assembly comprises a conical seat, a cavity is formed in the conical seat, a water inlet hole is formed between the cavity and the conical seat, and a water drain hole is formed in the bottom of the conical seat.

The center position department of toper seat opens there is the trompil, the output shaft is installed in the inside of trompil, it has the spacing groove to open on the inner wall of trompil, be fixed with the stopper on the output shaft, stopper slidable mounting is in the inside of spacing groove.

The connecting assembly comprises a connecting disc, a connecting seat is welded at the bottom of the connecting disc, an annular groove is formed in the inner wall of the opening, and the connecting seat is arranged in the annular groove.

Control valves are arranged on the air inlet pipe, the air outlet pipe and the drainage assembly, and sealing rings are arranged between the movable pipe and the inner wall of the supporting pipe.

The side of toper seat is fixed with solid fixed ring, gu fixed ring contacts with the inner wall of inner bag, gu fixed ring is hard rubber material.

The invention has the beneficial effects that: the bioreactor of the invention comprises a base; a tub body; a sealing plate; a support rod; a fixing seat; a movable seat; a fixing mechanism; a lifting mechanism; an air inlet pipe; an exhaust pipe; a drainage assembly; a motor case; a power mechanism; an output shaft; a stirring assembly; a connection assembly; a movable column; a heating rod; an inner container; a heating mechanism; a support tube; a movable tube; a seal ring; a connecting flange; a conical seat; a cavity; a water inlet hole; a fixing ring; a drain hole; a limit groove; a limiting block; a connecting disc; a connecting seat; a ring groove; and (5) opening holes.

1. This bioreactor, during the use, the inside toper seat of stirring subassembly cooperatees through inlet opening and wash port and stirs living beings, and the position of toper seat can be adjusted from top to bottom during the stirring, accomplishes stirring to the living beings in different positions and uses when adjusting from top to bottom, gets into living beings through the inlet opening and discharges from the wash port again, and living beings bump in inside and are favorable to stirring and use.

2. This bioreactor, when needs are to the quick cooling of organism, get into the organism in the toper seat cavity is inside, upwards remove the toper seat, remove highly be greater than the inside height of organism inner bag, the organism is poured down when forming the waterfall through the wash port, gets into through the air-supply line and bloies the organism this moment, accomplishes quick cooling, and the toper seat is rotatory when cooling, makes the organism pour and accomplishes stirring use.

3. This bioreactor installs heating mechanism in movable column and inner bag department, and heating mechanism heats simultaneously from the outside and the inside of organism, and under the stirring of rethread stirring subassembly, makes the biological quick heating of completion of different positions use, and the back conical seat of later stage biological discharge upwards moves, and the solid fixed ring on the conical seat is scraped the inside of inner bag and is rubbed, is scraped and is rubbed the inner wall clearance use of inner bag.

Drawings

FIG. 1 is a schematic view of a bioreactor according to the present invention;

FIG. 2 is a schematic view showing a cross-section of a barrel of a bioreactor according to the present invention;

FIG. 3 is an enlarged schematic view of a drainage assembly of a bioreactor according to the present invention;

FIG. 4 is a schematic structural view of a stirring assembly of a bioreactor according to the present invention;

FIG. 5 is a schematic perspective view of a conical seat of a bioreactor according to the present invention;

in the figure: 1. a base; 2. a tub body; 3. a sealing plate; 4. a support rod; 5. a fixing seat; 6. a movable seat; 7. a fixing mechanism; 8. a lifting mechanism; 9. an air inlet pipe; 10. an exhaust pipe; 11. a drainage assembly; 12. a motor case; 13. a power mechanism; 14. an output shaft; 15. a stirring assembly; 16. a connection assembly; 17. a movable column; 18. a heating plate; 19. an inner container; 20. a heating mechanism; 21. a support tube; 22. a movable tube; 23. a seal ring; 24. a connecting flange; 25. a conical seat; 26. a cavity; 27. a water inlet hole; 28. a fixing ring; 29. a drain hole; 30. a limit groove; 31. a limiting block; 32. a connecting disc; 33. a connecting seat; 34. a ring groove; 35. and (5) opening holes.

Detailed Description

The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.

Referring to fig. 1 to 5, the present invention provides a technical solution: the utility model provides a bioreactor, includes the bioreactor body, the bioreactor body includes base 1, be fixed with staving 2 on the base 1, slidable mounting has sealed dish 3 between staving 2 and the base 1, the top welding of staving 2 has fixing base 5, the top movable mounting of fixing base 5 has movable seat 6, be connected through fixed establishment 7 between movable seat 6 and the fixing base 5, be fixed with elevating system 8 on the movable seat 6, intake pipe 9 is installed to elevating system 8's avris, blast pipe 10 is installed to intake pipe 9's avris, blast pipe 10 and intake pipe 9 are all fixed on movable seat 6, the bottom of base 1 is fixed with motor case 12 and bracing piece 4, the internally mounted of motor case 12 has power unit 13, power unit 13 is fixed in the bottom of base 1, install output shaft 14 on the power unit 13, install stirring subassembly 15 on the output shaft 14, elevating system 8 is electric putter control stirring subassembly 15 is at the inside height of inner bag 19 to the switching of different positions biology.

In this embodiment, the inside of staving 2 is fixed with inner bag 19, stirring subassembly 15 installs the inside of inner bag 19, the inside of inner bag 19 contains heating mechanism 20, connecting assembly 16 is installed at stirring subassembly 15's top, be fixed with movable post 17 on the connecting assembly 16, heating plate 18 is installed to movable post 17's bottom, the one end of movable post 17 is installed on elevating system 8, install drainage subassembly 11 between inner bag 19 and the staving 2, drainage subassembly 11 includes stay tube 21, the one end of stay tube 21 is installed on inner bag 19, the inside slidable mounting of stay tube 21 has movable tube 22, the one end welding of movable tube 22 has flange 24, and movable tube 22 later contracts in the inside of stay tube 21, and rethread sealing disc 3 seals, avoids dust and bacterium to enter into the influence that drainage subassembly 11 caused.

In this embodiment, the stirring assembly 15 includes the conical seat 25, the inside of conical seat 25 is opened there is cavity 26, it has inlet opening 27 to open between cavity 26 and the conical seat 25, the bottom of conical seat 25 is opened there is wash port 29, the central point department of conical seat 25 is opened there is trompil 35, the inside at trompil 35 is installed to output shaft 14, it has spacing groove 30 to open on the inner wall of trompil 35, be fixed with stopper 31 on the output shaft 14, stopper 31 slidable mounting is in the inside of spacing groove 30, and stopper 31 slides from top to bottom in the inside of spacing groove 30, and conical seat 25 can change the altitude mixture rotation during power transmission.

In this embodiment, the coupling assembling 16 includes the connection pad 32, the bottom welding of connection pad 32 has connecting seat 33, open on the inner wall of trompil 35 has annular 34, connecting seat 33 installs the inside at annular 34, all install the control valve on intake pipe 9, blast pipe 10 and the drainage subassembly 11, install sealing ring 23 between the inner wall of movable pipe 22 and stay tube 21, the avris of toper seat 25 is fixed with solid fixed ring 28, solid fixed ring 28 contacts with the inner wall of inner bag 19, solid fixed ring 28 is hard rubber material, and biological removal back toper seat 25 upwards moves in the later stage, and gu fixed ring 28 on the toper seat 25 cuts the inner wall of inner bag 19 and rubs the inner wall clearance use of inner bag 19, and heating mechanism 20 and hot plate 18 are the electric heater strip and constitute, and the circular telegram is accomplished and is heated the use.

When the device is used, the sealing disk 3 is moved upwards on the barrel body 2, the water discharging assembly 11 is exposed after the movement, the movable pipe 22 is pulled out from the inside of the supporting pipe 21 through the connecting flange 24, biological raw materials enter the inside of the movable pipe 22 after being pulled out, the control valve on the supporting pipe 21 is closed after the biological raw materials enter, the movable pipe 22 is contracted in the supporting pipe 21, the sealing disk 3 descends to seal the water discharging assembly 11, the influence caused by dust and bacteria is avoided, then the heating mechanism 20 and the heating plate 18 are started, the heating rod 18 heats the living beings from inside to outside, the heating mechanism 20 heats the living beings from outside to inside, the two-side heating is convenient for the living beings in the inside to complete rapid temperature rise, the power mechanism 12 is started during the temperature rise heating, the power mechanism 12 is a direct current motor, drives the output shaft 14 to rotate, the output shaft 14 rotates in the limit groove 30 through the limit block 31, provides power for the rotating seat for the conical seat 25, when the conical seat 25 rotates, the water inlet 26 enters biological liquid through the rotating force, the biological liquid is discharged from the water inlet 26 under the centrifugal force of the conical seat 25 again, and circulates in sequence, so that stirring of the internal biological liquid is facilitated, when stirring, the lifting mechanism 8 is contracted, the conical seat 25 slides up and down on the output shaft 14 when the lifting mechanism is contracted, the conical seat 25 rotates on the connecting seat 33 when the lifting mechanism slides up and down, the height is changed while rotating, stirring and heating of the biological liquid at different height positions are completed, when cooling is required after heating is completed, the stirring assembly 15 stays at the bottom of the inner container 19 for a period of time, and rapidly moves up to the level of the biological liquid through the lifting mechanism 8, biological fluid is discharged outwards from the inside of inlet 27 and wash port 29, forms the waterfall shape when discharging, gets into air conditioning through intake pipe 9, cools off the biological fluid of waterfall shape, and the steam after the cooling passes through blast pipe 10 and discharges, and conical seat 25 is rotatory when cooling down, accomplishes the outflow and the inner bag 19 collision of messenger's organism and accomplishes the stirring again and use in the time of pouring.

While the fundamental and principal features of the invention and advantages of the invention have been shown and described, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. A bioreactor comprising a bioreactor body, characterized in that: the bioreactor body comprises a base (1), a barrel body (2) is fixed on the base (1), a sealing disc (3) is slidably arranged between the barrel body (2) and the base (1), a fixing seat (5) is welded at the top of the barrel body (2), a movable seat (6) is movably arranged at the top of the fixing seat (5), the movable seat (6) is connected with the fixing seat (5) through a fixing mechanism (7), a lifting mechanism (8) is fixed on the movable seat (6), an air inlet pipe (9) is arranged on the side of the lifting mechanism (8), an air outlet pipe (10) is arranged on the side of the air inlet pipe (9), the air outlet pipe (10) and the air inlet pipe (9) are both fixed on the movable seat (6), a motor case (12) and a supporting rod (4) are fixed at the bottom of the base (1), a power mechanism (13) is fixed at the bottom of the base (1), an output shaft (14) is arranged on the power mechanism (13), an inner container (19) is arranged on the inner container (15) and an inner container (19) is arranged on the inner container (19), the inside of inner bag (19) contains heating mechanism (20), coupling assembling (16) are installed at the top of stirring subassembly (15), be fixed with movable column (17) on coupling assembling (16), hot plate (18) are installed to the bottom of movable column (17), install on elevating system (8) one end of movable column (17), install drainage subassembly (11) between inner bag (19) and staving (2), drainage subassembly (11) include stay tube (21), the one end of stay tube (21) is installed on inner bag (19), the internally sliding mounting of stay tube (21) has movable tube (22), the one end welding of movable tube (22) has flange (24), stirring subassembly (15) are including conical seat (25), the inside of conical seat (25) is opened there is cavity (26), open between cavity (26) and conical seat (25) has inlet opening (27), the bottom of conical seat (25) is opened there is drainage hole (29), the one end of stay tube (21) is installed on inner wall (35) of trompil (35) in the position of cone seat (25), the novel high-speed motor is characterized in that a limiting block (31) is fixed on the output shaft (14), the limiting block (31) is slidably mounted in the limiting groove (30), the connecting assembly (16) comprises a connecting disc (32), a connecting seat (33) is welded at the bottom of the connecting disc (32), an annular groove (34) is formed in the inner wall of the opening (35), and the connecting seat (33) is mounted in the annular groove (34).

2. A bioreactor according to claim 1, wherein: control valves are arranged on the air inlet pipe (9), the air outlet pipe (10) and the drainage assembly (11), and a sealing ring (23) is arranged between the movable pipe (22) and the inner wall of the supporting pipe (21).

3. A bioreactor according to claim 1, wherein: the side of the conical seat (25) is fixedly provided with a fixing ring (28), the fixing ring (28) is in contact with the inner wall of the liner (19), and the fixing ring (28) is made of hard rubber.