CN115957372A - Antibacterial healing-promoting hydrogel dressing rich in blumea oil and preparation method and application thereof - Google Patents
Antibacterial healing-promoting hydrogel dressing rich in blumea oil and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to an antibacterial healing-promoting hydrogel dressing rich in blumea oil and a preparation method thereof, belonging to the technical field of biological material preparation. The antibiotic dressing overcomes the problem that the existing antibiotic dressing is easy to cause the risk of drug-resistant strains and the heavy metal biotoxicity of silver ion dressing, and can not meet the clinical application requirement. According to the invention, methacrylic acid anhydrified gelatin is used as a matrix material, acrylamide and acrylic acid N-succinimide are used as polymerization monomers, an blumea oil-in-water Pickering emulsion template method is utilized, the blumea oil-loaded hydrogel dressing is prepared through free radical polymerization, the dressing strength is convenient to regulate, the blumea oil active component is loaded in the gel in a liquid drop form, a good mass transfer channel is provided, the functional component can rapidly reach the wound surface, and the rapid antibacterial and anti-inflammatory effects are achieved.
Description
Technical Field
The invention relates to an antibacterial healing-promoting hydrogel dressing rich in blumea oil, and a preparation method and application thereof, and belongs to the technical field of biological material preparation.
Background
The pain and harm to patients caused by chronic wounds caused by trauma and chronic diseases are one of the important clinical challenges. Bacterial infection is one of the most major factors affecting wound healing. When a wound is infected, bacteria may cause a persistent inflammatory response at the infected site, increasing the production of wound exudate, which is a hotbed for the bacteria to provide more nutrients for bacterial growth, thus creating a vicious circle between the bacterial infection and the wound exudate. A mild bacterial infection delays the wound healing process, while a severe infection not only results in difficult wound healing, but may even lead to complications such as sepsis and the like. In addition, it is noted that chronic wounds are mostly resistant to bacterial infections. Therefore, the ideal novel chronic wound dressing must have excellent antibacterial performance. However, gauze, sponge, silver ion dressing, antibiotic dressing and the like which are commonly used in clinical at present show obvious defects and shortcomings in the clinical treatment of chronic wounds: for example, gauze has poor moisture retention, does not have antibacterial performance, and is easy to cause tissue adhesion; the sponge is easy to drain and block and causes tissue adhesion; the silver ion dressing has the risk of heavy metal biotoxicity; antibiotic dressing is easy to cause drug-resistant strains and the like. The development of novel antibacterial dressings, the solution of the biological toxicity of antibiotic resistant bacteria and heavy metal antibacterial agents is an important direction for the development of wound dressings, and has important clinical value.
The active compound of folium Artemisiae Argyi, oleum Artemisiae Argyi, has effects of easily permeating into skin, activating blood circulation, eliminating swelling, removing blood stasis, relieving pain, and promoting necrotic tissue exfoliation, and has various pharmacological activities, such as antibacterial, antiinflammatory, antiallergic, antiviral and complement activation, and can also reduce tissue edema and promote granulation growth of wound tissue. However, the application of the blumea oil has some limitations, such as poor water solubility and high volatility, which causes low bioavailability and greatly limits the application. Chinese patent CN1l4569784A discloses a folium artemisiae argyi extract-loaded hydrogel and a preparation method thereof, wherein mesoporous silica is adopted to load folium artemisiae argyi extract, then the mesoporous silica loaded with folium artemisiae argyi oil is mixed into methacrylated gelatin and methacrylated hyaluronic acid containing a photoinitiator, and finally the folium artemisiae argyi extract-loaded hydrogel is prepared through photoinitiation. The method has positive correlation between the loading capacity of the functional component blumea oil and the addition amount of the mesoporous silica, and the dressing strength is increased and the dressing becomes hard due to the excessively high addition amount of the silica, so that the use comfort of the dressing is influenced, and therefore, the regulation and control of the functional component have limitation. In addition, because the functional components are loaded in the mesoporous silica and are embedded in the gel, mass transfer channels are blocked, release is blocked, and the dressing is usually replaced once every 2-3 days, which undoubtedly affects the action effect of the functional components.
Disclosure of Invention
In order to overcome the problem that the existing antibiotic antibacterial dressing cannot meet the requirement of clinical application because of easily causing the risk of heavy metal biotoxicity of drug-resistant strains and silver ion dressings, the invention provides the antibacterial healing-promoting hydrogel dressing rich in the blumea oil and the preparation method thereof.
The blumea oil-rich hydrogel dressing for resisting bacteria and promoting healing is prepared by taking methacrylic anhydride gelatin as a base material, taking acrylamide and acrylic acid N-succinimide as polymerization monomers and utilizing a blumea oil-in-water emulsion template method through free radical polymerization, the strength of the dressing and antibacterial active components are convenient to regulate and control, the blumea oil active components are loaded in the gel in a liquid drop form, a good mass transfer channel is formed, the functional components can quickly reach the wound surface, and the hydrogel dressing is quick in resisting bacteria and resisting inflammation. Wherein the mugwort leaf oil-in-water emulsion template method is a mugwort leaf oil-in-water Pickering emulsion template method. The blumea oil-in-water Pickering emulsion template method is the blumea oil-in-water Pickering emulsion template method.
The invention also provides a preparation method of the antibacterial healing-promoting hydrogel dressing rich in the blumea oil, which comprises the following steps:
step 1, dissolving attapulgite and tween 20 in deionized water, and stirring to uniformly disperse; then dripping the blumea oil, stirring and emulsifying to form emulsion;
step 2, adding acrylamide, methacrylic acid anhydrified gelatin, acrylic acid N-hydroxysuccinimide and ammonium persulfate into the emulsion obtained in the step 1, and uniformly stirring;
and 3, initiating the solution in the step 2 to perform free radical polymerization reaction to obtain the antibacterial healing-promoting hydrogel dressing rich in the blumea oil.
Further, in the step 1, the mass ratio of the attapulgite, the tween-20, the deionized water and the blumea oil is (0.2-0.5).
Preferably, the mass ratio of the attapulgite, the tween 20, the deionized water and the blumea oil in the step 1 is 0.2.
Preferably, in the step 1, the attapulgite and the tween 20 are dissolved in deionized water and stirred to be uniformly dispersed; stirring and dispersing at 2000-4000rpm for 0.5-2min, adding oleum folium Artemisiae Argyi dropwise, stirring and emulsifying at 8000-11000rpm for 5-8min to form emulsion.
Preferably, in the step 3, the solution obtained in the step 2 is placed in an oven with the temperature of 55-65 ℃ to initiate the solution to perform free radical polymerization reaction, and the reaction time is 90-150min. If the reaction time is too short, incomplete polymerization will be caused, and if the reaction time is too long, the amount of volatile components in the blumea oil and water will increase.
Further, the sum of the mass of the deionized water and the mass of the blumea oil in the step 1 is A, and the mass of the acrylamide, the mass of the methacrylic anhydrized gelatin, the mass of the acrylic acid N-hydroxysuccinimide and the ratio of the mass of the ammonium persulfate to the mass of the blumea oil in the step 2 are respectively as follows: 0.2-0.5.
Further, the mass of acrylamide, the mass of methacrylic anhydrified gelatin, the mass of acrylic acid N-hydroxysuccinimide and the ratio of the mass of ammonium persulfate to a added in step 2 are respectively: 0.3.
An application of antibacterial healing-promoting hydrogel dressing rich in folium artemisiae argyi oil in the application of medical dressing on skin wound.
The invention also provides the antibacterial healing-promoting hydrogel dressing rich in the blumea oil, which is prepared by the method.
The invention has the beneficial effects that:
1. according to the invention, the natural antibacterial agent blumea oil is used as an oil phase to prepare a blumea oil-in-water emulsion, and then the composite hydrogel carrying blumea oil is constructed through the free radical polymerization of methacrylic acid anhydrified gelatin, acrylamide and acrylic acid N-succinimide in the emulsion continuous phase. The blumea oil is loaded into the hydrogel, so that the stability of the blumea oil can be effectively improved, and the volatility of the pure blumea oil is reduced. In addition, the active components of the blumea oil are loaded in the gel in a liquid drop form, a good mass transfer channel is provided, the functional components can rapidly reach the wound surface, and the blumea oil gel is rapid in antibiosis and anti-inflammatory.
2. The invention adopts the pure natural antibacterial agent of the blumea oil, does not cause the drug resistance of bacteria, avoids the biotoxicity of metal ions, and meets the requirement of clinical application.
3. The method can conveniently regulate and control the loading content of the blumea oil by regulating and controlling the emulsification condition, and the loading capacity can be regulated and controlled between 5 percent and 80 percent.
4. The hydrogel dressing disclosed by the invention has good antibacterial performance and good moisturizing performance, can effectively keep the wound surface moist, enables the wound to be in a stable moist state for a long time, is not easy to adhere to the wound surface, does not cause secondary damage to the wound surface during dressing change, can effectively promote the wound surface to heal, and promotes skin regeneration. Moreover, the hydrogel dressing does not react with biological tissues and has no irritation to the biological tissues.
5. The hydrogel dressing prepared by the method has good seepage absorption capacity and can effectively absorb the seepage of the wound surface.
Drawings
FIG. 1 is a photograph of an emulsion of an oil of mugwort leaf in water stabilized by attapulgite and Tween 20 in the example;
FIG. 2 is a dispersion of an emulsion of blumea oil-in-water in water and in toluene, wherein the dispersion of the emulsion in water is shown on the left; the right side shows the dispersion condition of the emulsion in toluene;
FIG. 3 is a photomicrograph of the example prepared esquirite-oil-enriched antimicrobial healing-promoting hydrogel dressing;
FIG. 4 is a graph of the in vitro antimicrobial effect of the blumea oil-rich antimicrobial healing-promoting hydrogel dressing prepared in the examples;
FIG. 5 is a biocompatibility experiment of the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing prepared in the examples;
FIG. 6 is a diagram of experimental healing-promoting effects of the antibacterial healing-promoting hydrogel dressing animal prepared in the example and rich in the oleum folium Artemisiae Argyi.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying figures of the present invention are described in detail below, and it is apparent that the described embodiments are a part, not all or all of the embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
According to the invention, methacrylic acid anhydrified gelatin is used as a matrix material, acrylamide and acrylic acid N-succinimide are used as polymerization monomers, an blumea oil-in-water Pickering emulsion template method is utilized, the blumea oil-loaded hydrogel dressing is prepared through free radical polymerization, the blumea oil is loaded into the hydrogel, the stability of the blumea oil can be effectively improved, and meanwhile, compared with the blumea extract-loaded hydrogel, the blumea oil-loaded hydrogel has a good mass transfer channel, so that the blumea oil can smoothly reach a wound surface, and a long-acting antibacterial function is exerted. The preparation process of the present invention will be described in detail with reference to specific examples.
Example 1
This example prepares an antibacterial healing-promoting hydrogel dressing 1 rich in blumea oil by:
1) Emulsion synthesis: dissolving 0.25g of attapulgite and 0.2g of Tween 20 in 9.5g of deionized water, and dispersing at 3000rpm for 1min; then 0.5g of blumea oil is dripped, and emulsified for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.3, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.02, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.01 and ammonium persulfate with the mass ratio to the A of 0.05 into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in a drying oven at 60 ℃ for reaction for 2h to obtain the antibacterial healing-promoting hydrogel rich in the blumea oil; the macro-topography of the prepared hydrogel dressing 1 is shown in fig. 3.
Example 2
This example prepares an antibacterial healing-promoting hydrogel dressing 2 rich in blumea oil by:
1) Emulsion synthesis: dissolving 0.25g of attapulgite and 0.2g of Tween 20 in 9g of deionized water, and dispersing at 3000rpm for 1min; then 1g of blumea oil is dripped in the mixture and emulsified for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.3, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.02, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.01 and ammonium persulfate with the mass ratio to the A of 0.05 into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the moxa oil-rich hydrogel dressing 2 capable of resisting bacteria and promoting healing.
Example 3
This example prepares an antibacterial healing-promoting hydrogel dressing 3 rich in blumea oil by:
1) Synthesizing an emulsion: dissolving 0.25g of attapulgite and 0.2g of Tween 20 in 8g of deionized water, and dispersing at 3000rpm for 1min; then dripping 2g of blumea oil, emulsifying for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio of 0.3 to A, methacrylic acid anhydrified gelatin with the mass ratio of 0.02 to A, acrylic acid N-hydroxysuccinimide with the mass ratio of 0.01 to A and ammonium persulfate with the mass ratio of 0.05 to A into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the moxa oil-rich hydrogel dressing 3 capable of resisting bacteria and promoting healing.
Example 4
This example prepares an antibacterial healing-promoting hydrogel dressing 4 rich in adeps artemisiae argyi by:
1) Synthesizing an emulsion: dissolving 0.25g of attapulgite and 0.2g of Tween 20 in 6g of deionized water, and dispersing at 3000rpm for 1min; then 4g of blumea oil is dripped in the mixture and emulsified for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.3, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.02, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.01 and ammonium persulfate with the mass ratio to the A of 0.05 into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the moxa oil-rich hydrogel dressing 4 capable of resisting bacteria and promoting healing.
Example 5
This example prepares an antibacterial healing-promoting hydrogel dressing 5 rich in blumea oil by:
1) Synthesizing an emulsion: dissolving 0.25g of attapulgite 0.2g of Tween 20 in 4g of deionized water, and dispersing at 3000rpm for 1min; then 6g of blumea oil is dripped in the mixture and emulsified for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.3, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.02, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.01 and ammonium persulfate with the mass ratio to the A of 0.05 into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the moxa oil-rich hydrogel dressing 5 capable of resisting bacteria and promoting healing.
Example 6
This example prepares an antibacterial healing-promoting hydrogel dressing 6 rich in adeps artemisiae argyi by:
1) Synthesizing an emulsion: dissolving 0.25g of attapulgite 0.2g of Tween 20 in 2g of deionized water, and dispersing at 3000rpm for 1min; then 8g of blumea oil is dripped in the mixture and emulsified for 5min at 11000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.3, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.02, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.01 and ammonium persulfate with the mass ratio to the A of 0.05 into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the moxa oil-rich hydrogel dressing 6 capable of resisting bacteria and promoting healing.
Example 7
This example prepares an antibacterial healing-promoting hydrogel dressing 7 rich in adeps artemisiae argyi by:
1) Synthesizing an emulsion: 0.5g of attapulgite and 0.2g of Tween 20 are dissolved in 6g of deionized water and dispersed for 0.5min at 4000 rpm; then 4g of blumea oil is added dropwise and emulsified for 6.5min at 10000rpm to form emulsion;
2) Adding acrylamide with the mass ratio to the A of 0.5, methacrylic acid anhydrified gelatin with the mass ratio to the A of 0.03, acrylic acid N-hydroxysuccinimide with the mass ratio to the A of 0.03 and ammonium persulfate with the mass ratio to the A of 0.01 into the emulsion, and uniformly stirring;
3) Putting the solution obtained in the step 2) into an oven at 55 ℃ for reaction for 150min to obtain the antibacterial healing-promoting hydrogel dressing 7 rich in the blumea oil.
Example 8
This example prepares an antibacterial healing-promoting hydrogel dressing 8 rich in blumea oil by:
1) Emulsion synthesis: dissolving 0.2g of attapulgite 0.2g of Tween 20 in 7g of deionized water, and dispersing at 2000rpm for 2min; then 3g of argy wormwood leaf oil is dripped in, and emulsification is carried out for 8min at 8000rpm to form emulsion;
2) Adding acrylamide with the mass ratio of 0.2 to A, methacrylic acid anhydrified gelatin with the mass ratio of 0.04 to A, acrylic acid N-hydroxysuccinimide with the mass ratio of 0.02 to A and ammonium persulfate with the mass ratio of 0.03 to A into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in a drying oven at 65 ℃ for reaction for 90min to obtain the moxa oil-rich hydrogel dressing 8 capable of resisting bacteria and promoting healing.
Comparative example 1:
1) Synthesizing an emulsion: dissolving 0.25g of attapulgite and 0.2g of Tween 20 in 10g of deionized water, and dispersing at 3000rpm for 1min;
2) Adding acrylamide with the mass ratio to water of 30%, methacrylic acid anhydridized gelatin with the mass ratio of 2%, acrylic acid N-hydroxysuccinimide with the mass ratio of 1% and ammonium persulfate with the mass ratio of 5% into the emulsion, and uniformly stirring;
3) Placing the solution obtained in the step 2) in an oven at 60 ℃ for reaction for 2h to obtain the hydrogel dressing 9 without the blumea oil;
in order to verify the successful preparation of the antibacterial healing-promoting hydrogel dressing rich in the blumea oil and the performances of the hydrogel dressing in the aspects of antibiosis and wound healing promotion, the following performance tests are carried out.
1. Experimental procedures
(1) Oil-in-water emulsion demonstration experiment: first, an emulsion was prepared according to the above test method, and then the emulsion was dropped into water and toluene, respectively, and the dispersion of the emulsion in water and toluene, respectively, was observed.
(2) In vitro antibacterial experiments of dressings: all samples to be tested (samples of the hydrogel dressing 1 to 6 were selected) were first sterilized. Inoculating 1-2 colonies of staphylococcus aureus, escherichia coli stock solution and methicillin-resistant staphylococcus aureus to 30mL of liquid culture medium, and performing shake culture on a constant-temperature shaking table at 37 ℃ for 14-16 hours to activate. Then preparing bacterial suspension, adjusting the bacterial concentration to 10 by turbidimeter 7 CFC/mL. The sample is placed in a 48-well plate, 300 microliter of bacterial suspension is added into each well, and the mixture is cultured for 8-12h. And finally, diluting and coating: taking the co-culture supernatant, diluting by one million times, plating, culturing for 12 hours, photographing and recording data.
(3) Cell compatibility experiments of dressings: in order to evaluate the biosafety of the adjuvant, a biocompatibility experiment was performed by co-culturing mouse epithelial-like fibroblast cells L929 with the hydrogel dressing 1 and the hydrogel dressing 2 prepared in the present invention. The experimental process is as follows: firstly, the dressing is soaked in a DMEM culture medium for 24 hours, and the proportion of the soaking solution is 2cm 2 1mL of a culture medium with the blumea oil dressing; then preparing cell suspension, counting cells, inoculating the cells into 96-well plate, wherein the number of cells per well is about more than or equal to 5 × 10 4 (ii) a Then adding 100uL dressing leaching liquor into each cell pore plate, and co-culturing for 24h in a 37 ℃ incubator; then, the cell morphology was observed under a microscope, and then, 10uL of CCK8 was added thereto and cultured for 2 hours, and the cell viability was calculated by measuring the absorbance at a wavelength of 450 nm.
(4) Animal experiments: adult SD rats were selected as test subjects, and SD rats were anesthetized with 3wt% sodium pentobarbital (0.1-0.15 mL/100 g) under aseptic conditions, back hairs were shaved off, a drape was sterilized, a model of a back skin wound surface of about 1cm in diameter was constructed under aseptic conditions, and then various dressings were covered on the surface of the wound surface and fixed with adhesive tape. Rats covered by the hydrogel dressing 1 and the hydrogel dressing 2 are selected as an experimental group, rats covered by the hydrogel dressing 9 are selected as a control group, and rats not covered by any dressing are selected as a blank group. The skin wound on the back of the rat was photographed on days 1, 3, 5, 7, 10, 12, and 14 to observe the healing.
2. Results of the experiment
(1) Oil-in-water emulsion proof of experiment and dressing macro-photograph: FIG. 1 is a photomicrograph of an emulsion. FIG. 2 is a verification experiment of emulsion type, as shown in FIG. 2, the emulsion is easy to disperse after dropping into water, but the emulsion is dropped into toluene and directly deposited on the bottom of the container, and is not easy to disperse, which indicates that the prepared emulsion is an oil-in-water emulsion, and thus the blumea oil can be successfully introduced into the hydrogel based on the oil-in-water emulsion. Fig. 3 is a photomicrograph of a dressing made according to the present invention.
(2) In vitro antibacterial experiments: fig. 4 is the results of in vitro antibacterial performance tests of the hydrogel dressings with different blumea oil concentrations prepared in examples 1 to 6 on staphylococcus aureus, escherichia coli and methicillin-resistant staphylococcus aureus, and it can be seen from the figure that the antibacterial effects of the control group without blumea oil on staphylococcus aureus, escherichia coli and methicillin-resistant staphylococcus aureus are very limited, while the antibacterial effects of the hydrogel containing blumea oil on staphylococcus aureus, escherichia coli and methicillin-resistant staphylococcus aureus are gradually enhanced with the increase of the blumea oil concentration, and the antibacterial rates of the hydrogel dressing 1-hydrogel dressing 6 on staphylococcus aureus and methicillin-resistant staphylococcus aureus reach 100%; the antibacterial rate to the escherichia coli is higher than 90%.
(3) Cell compatibility: according to the result of the antibacterial experiment, the hydrogel dressing 1 and the hydrogel dressing 2 are selected to represent the biocompatibility, as shown in fig. 5, the cell survival rates of the hydrogel dressing 1 and the hydrogel dressing 2 prepared by the invention are both greater than 90%, which indicates that the dressing prepared by the invention has good biocompatibility.
(4) Animal experiments: as can be seen from FIG. 6, the wound covered by the dressing prepared by the invention heals faster than the blank group and the control group, which shows that the dressing prepared by the invention has good antibacterial and healing promoting effects.
(5) The moxa oil-rich antibacterial healing-promoting hydrogel dressing 7 and the moxa oil-rich antibacterial healing-promoting hydrogel dressing 8 have good seepage absorption capacity and can effectively absorb wound seepage.
Claims (9)
1. The antibacterial healing-promoting hydrogel dressing rich in the blumea oil is characterized in that: the preparation method is characterized in that methacrylic acid anhydrified gelatin is used as a base material, acrylamide and acrylic acid N-succinimide are used as polymerization monomers, and the preparation method is characterized in that the preparation method is prepared by free radical polymerization by using an oil emulsion template method in water of folium artemisiae argyi.
2. The preparation method of the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to claim 1, comprising the following steps:
step 1, dissolving attapulgite and tween 20 in deionized water, and stirring to uniformly disperse the attapulgite and tween; then dripping the blumea oil, stirring and emulsifying to form emulsion;
step 2, adding acrylamide, methacrylic acid anhydrified gelatin, acrylic acid N-hydroxysuccinimide and ammonium persulfate into the emulsion obtained in the step 1, and uniformly stirring;
and 3, initiating the solution in the step 2 to perform free radical polymerization reaction to obtain the antibacterial healing-promoting hydrogel dressing rich in the blumea oil.
3. The method of preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing of claim 2, wherein: in the step 1, the mass ratio of the attapulgite, the tween 20, the deionized water and the blumea oil is 0.2-0.5.
4. The method of preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to claim 3, wherein: in step 1, the mass ratio of attapulgite, tween 20, deionized water and blumea oil is 0.2.
5. The method of preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to any one of claims 2 to 4, wherein: dissolving attapulgite and tween 20 in deionized water in the step 1, and stirring to uniformly disperse the attapulgite and tween; stirring and dispersing at 2000-4000rpm for 0.5-2min, adding oleum folium Artemisiae Argyi dropwise, stirring and emulsifying at 8000-11000rpm for 5-8min to form emulsion.
6. The method for preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to any one of claims 2 to 4, wherein the method comprises the following steps: and 3, placing the solution obtained in the step 2 in an oven at 55-65 ℃ to initiate the solution to perform free radical polymerization reaction for 90-150min.
7. The method of preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to any one of claims 2 to 4, wherein: the sum of the mass of the deionized water and the mass of the blumea oil in the step 1 is A, and the mass of the acrylamide, the mass of the methacrylic anhydride gelatin, the mass of the acrylic acid N-hydroxysuccinimide and the ratio of the mass of the ammonium persulfate to the mass of the blumea oil in the step 2 are respectively as follows: 0.2-0.5.
8. The method for preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing according to claim 7, wherein the step of preparing the moxa oil-enriched antimicrobial healing-promoting hydrogel dressing comprises the following steps: the mass of acrylamide, the mass of methacrylic anhydrified gelatin, the mass of acrylic acid N-hydroxysuccinimide and the mass ratio of ammonium persulfate to A added in the step 2 are respectively as follows: 0.3.
9. The application of the blumea oil-rich antibacterial healing-promoting hydrogel dressing is characterized in that: the antibacterial healing-promoting hydrogel dressing rich in the blumea oil is applied as a medical dressing to skin wounds.
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