CN115931809A - Optical detection device for PCR instrument - Google Patents

Abstract

The invention discloses an optical detection device for a PCR instrument, which comprises a light source assembly, a light homogenizing plate, a plurality of first lenses, a plurality of second lenses, a converging lens, a filtering component and a collecting component, wherein the light source assembly is used for emitting exciting light and emitting the exciting light into the light homogenizing plate; after the exciting light got into even worn-out fur, the exciting light can be through first reflectance coating and second reflectance coating mutual reflection, then kicked into the sample tube in, the sample to be measured in the sample tube excites the emission light, and the emission light is kicked into the acquisition component, is detected by the acquisition component to accomplish the optical detection of the sample to be measured in the sample tube, avoided the difference of testing result, improve the reliability of testing result.

Description

Optical detection device for PCR instrument

Technical Field

The invention relates to the technical field of optical detection, in particular to an optical detection device for a PCR instrument.

Background

When a PCR instrument is used to perform qualitative and quantitative analysis on a sample to be detected, a light source emits excitation light, and the excitation light irradiates the processed sample to be detected, so that fluorescence, i.e., emission light, is excited from the sample to be detected, and the excited fluorescence is collected by the detection instrument, and then the sample to be detected is qualitatively and quantitatively analyzed according to the collection result.

In the prior art, when a plurality of same samples to be detected are subjected to qualitative and quantitative detection analysis in the same batch, detection results are different, and the difference is mainly caused in two aspects; on one hand, when a plurality of samples to be detected are subjected to optical detection, the same light source is used for sequentially irradiating the samples to be detected, and the difference of final detection results is caused by the difference of time intervals and the difference of irradiation angles; on the other hand, when all samples to be measured are irradiated simultaneously using the same light source, although the influence of the time interval is reduced and the cost is controlled, the excitation light reaching each sample to be measured is still different, so that the final detection result is different and the reliability of the detection result is lowered.

Disclosure of Invention

The invention aims to provide an optical detection device for a PCR instrument, which solves the problem of low reliability of detection results.

In order to achieve the purpose, the invention provides a technical scheme that:

an optical detection device for a PCR instrument, comprising:

a light source assembly for emitting excitation light;

the light source assembly is arranged on at least one side of the light homogenizing plate, so that the exciting light is emitted into the light homogenizing plate, a sample tube is arranged below the light homogenizing plate, the exciting light is emitted into the sample tube through the light homogenizing plate, and a sample to be detected in the sample tube is excited to emit emitting light; the light homogenizing plate comprises a first light barrier and a second light barrier positioned below the first light barrier, the first light barrier is provided with a plurality of first light transmission holes, the second light barrier is provided with a plurality of second light transmission holes, a first reflecting film is arranged below the first light barrier, a second reflecting film is arranged above the second light barrier, the first reflecting film is provided with a plurality of light receiving holes, and the second reflecting film is provided with a plurality of light inlet holes;

the plurality of first lenses are positioned between the light homogenizing plate and the sample tube and are arranged in one-to-one correspondence with the sample tube;

the second lenses are positioned above the light homogenizing plate and are arranged in one-to-one correspondence with the sample tubes;

a condensing lens located above the plurality of second lenses for condensing the emitted light;

a filter member located above the converging lens for obtaining light of a desired wavelength band in the emitted light;

a collection member through which the emitted light passes to enter the collection member.

Optionally, the first lens, the first light hole, the second light hole, the light receiving hole, the light inlet, and the second lens are coaxially disposed.

Optionally, the aperture of the first light-transmitting hole is smaller than or equal to the aperture of the light-receiving hole, the aperture of the light-receiving hole is smaller than the aperture of the second light-transmitting hole, and the aperture of the second light-transmitting hole is smaller than or equal to the aperture of the light-entering hole.

Optionally, the backlight module further comprises a first mounting plate, the first mounting plate is located below the light homogenizing plate, the first mounting plate is provided with a plurality of first grooves and a plurality of second grooves located below the first grooves, and the size of the first grooves is smaller than that of the second grooves; a constant temperature plate is arranged below the first mounting plate, a plurality of first through holes are formed in the constant temperature plate, and the first through holes and the first lenses are arranged in a one-to-one correspondence manner; the first lens is positioned between the second groove and the first through hole.

Optionally, the light source further comprises a second mounting plate, the second mounting plate is located above the light homogenizing plate, the second mounting plate is provided with a plurality of third grooves and a plurality of fourth grooves located above the third grooves, and the size of the third grooves is smaller than that of the fourth grooves; a limiting plate is arranged above the second mounting plate, the limiting plate is provided with a plurality of second through holes, and the second through holes and the second lenses are arranged in a one-to-one correspondence manner; the second lens is positioned between the fourth groove and the second through hole.

Optionally, when the collection component is set as a shooting piece, the optical detection device further includes a first lens and a first filter wheel located above the first lens, the first filter wheel includes at least one first accommodation space, the filter component is located in the first accommodation space, the filter component is coaxial with the shooting piece, and the emitted light passes through the first lens and the filter component enters the shooting piece.

Optionally, when the collection part sets up to the hyperspectral spectrometer, optical detection device still includes speculum, second camera lens and is located the second filter wheel of second camera lens one side, the second filter wheel includes at least one second accommodation space, filter part is located in the second accommodation space, filter part with the second camera lens with the coaxial setting of hyperspectral meter, the transmission light passes through the speculum reflects, and passes through filter part with the camera lens jets into the hyperspectral spectrometer.

Optionally, the first reflective film includes a diffuser film or a brightness enhancement film, and the second reflective film includes a diffuser film or a brightness enhancement film.

Optionally, the light source assembly includes a substrate, a rotating shaft is arranged on the substrate, a rotating disc is rotatably connected to the rotating shaft, and a plurality of laser light sources are distributed on the rotating disc.

Optionally, two light source assemblies are arranged, and the two light source assemblies are symmetrically arranged on two sides of the light uniformizing plate.

Compared with the prior art, the invention has the beneficial effects that:

1. the optical detection device for the PCR instrument comprises a light source assembly, a light homogenizing plate, a plurality of first lenses, a plurality of second lenses, a converging lens, a filtering component and a collecting component, wherein the light source assembly is used for emitting exciting light, the exciting light is emitted into the light homogenizing plate, the exciting light is emitted into a sample tube through the light homogenizing plate, then a sample to be detected in the sample tube is excited to emit emitting light, the first lenses are positioned between the light homogenizing plate and the sample tube and are arranged in one-to-one correspondence with the sample tube, the second lenses are positioned above the light homogenizing plate and are arranged in one-to-one correspondence with the sample tube, the converging lens is positioned above the second lenses and is used for converging the emitting light, and the emitting light passes through the filtering component and is emitted into the collecting component; the light homogenizing plate comprises a first light barrier and a second light barrier, a plurality of first light holes are formed in the first light barrier, a plurality of second light holes are formed in the second light barrier, a first reflecting film is arranged below the first light barrier, a second reflecting film is arranged above the second light barrier, a plurality of light receiving holes are formed in the first reflecting film, a plurality of light inlet holes are formed in the second reflecting film, exciting light can be reflected mutually through the first reflecting film and the second reflecting film after entering the light homogenizing plate, then sequentially enters the light inlet holes, the second light holes and the first lens and enters the sample tube, a to-be-detected sample in the sample tube excites emitting light, one part of the emitting light enters the light homogenizing plate through the first lens, the second light holes, the light inlet holes, the light receiving holes, the first light holes, the second lens, the collecting lens and the filtering component, and the other part of the emitting light enters the light homogenizing plate through the first lens, the second light holes and the light inlet holes, the collecting lens, the second light collecting lens, the collecting lens and the filtering component, and the collecting component are analyzed. In addition, after the emitted light is converged by the second lens and the converging lens, the emitted light is filtered by the filter component to obtain the light with the required wave band, and the light is emitted into the acquisition component to be acquired and analyzed. Through the optical detection device of this application, make all sample tubes can receive the exciting light simultaneously, and the exciting light of receiving all approaches to evenly, unanimously, not only avoided among the prior art a plurality of samples that await measuring to have the result difference problem that time interval arouses because of receiving the exciting light, on the other hand, still make the exciting light that arrives all sample tubes approach to evenly, unanimously, thereby the difference of testing result has been avoided, the reliability of testing result has been improved, still gather the back through convergent lens and collection part with all emitted light and carry out the collection analysis at same moment in addition, the existence of the factor that probably influences the testing result has further been avoided, for example there is the time interval's factor when gathering the emitted light that different samples that await measuring arouse.

2. The filter component of the optical detection device for the PCR instrument is used for filtering the emitted light to obtain the light with the required wave band in the emitted light, and the emitted light is the light with a certain wave band range, and the light energy of different wave bands is different, so that the wave band with the strongest light energy in the emitted light can be captured by the filter component.

3. The aperture of the first light hole of the optical detection device for the PCR instrument is smaller than or equal to that of the light receiving hole, the aperture of the light receiving hole is smaller than that of the second light hole, and the aperture of the second light hole is smaller than or equal to that of the light inlet hole. Because the aperture of the light inlet hole is larger than that of the light receiving hole, the exciting light emitted upwards by the second reflecting film is less, the probability that the exciting light enters the acquisition component through the light receiving hole and is acquired is reduced, and the interference on the acquisition result of the subsequent emitted light is less; the aperture of the light receiving hole is larger than or equal to that of the first light transmitting hole, at the moment, the first light barrier can completely block light emitted by the first reflecting film, redundant light is prevented from entering the filtering component, the aperture of the light inlet hole is larger than or equal to that of the second light transmitting hole, at the moment, the second light barrier can completely block light emitted by the second reflecting film, redundant exciting light is prevented from irradiating the outer wall of the sample tube to enter the sample tube, and therefore exciting light entering a plurality of sample tubes is easy to control and keeps consistent.

4. The optical detection device for the PCR instrument can prevent the liquefied drops formed when the sample to be detected in the sample tube is evaporated at high temperature from being attached to the first lens to influence the light paths of the excitation light and the emission light through the arrangement of the thermostatic plate.

5. The light source assembly of the optical detection device for the PCR instrument comprises a substrate, wherein a rotating shaft is arranged on the substrate, a rotating disc is rotatably connected onto the rotating shaft, a plurality of laser light sources are distributed on the rotating disc, and different laser light sources can be aligned to a light homogenizing plate one by driving the rotating disc to rotate, so that the switching of different laser light sources is realized, and different detection requirements are met.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

FIG. 1 is a schematic diagram of an optical inspection apparatus according to the present invention;

FIG. 2 is a partial enlarged view of the optical inspection device of the present invention;

FIG. 3 is a simplified diagram of the related structure and optical path of a sample tube in the optical detection apparatus according to the present invention;

FIG. 4 is a schematic structural diagram of a light homogenizing plate corresponding to a sample tube in the optical inspection apparatus of the present invention;

FIG. 5 is a schematic structural diagram of a first reflective film corresponding to a sample tube in the optical detection apparatus of the present invention;

FIG. 6 is a schematic structural diagram of a second reflective film corresponding to a sample tube in the optical detection apparatus of the present invention;

FIG. 7 is a simplified schematic diagram of an optical inspection apparatus according to the present invention;

FIG. 8 is another simplified schematic diagram of an optical inspection apparatus according to the present invention;

FIG. 9 is a schematic structural diagram of a light source assembly of the optical detection apparatus of the present invention.

In the figure:

1. a light source assembly; 1a, a substrate; 1b, a rotating shaft; 1c, a turntable; 1d, a laser light source; 2. a light homogenizing plate; 21. a first light barrier; 211. a first light-transmitting hole; 22. a second light blocking plate; 221. a second light-transmitting hole; 23. a first reflective film; 231. a light-receiving hole; 24. a second reflective film; 241. a light inlet hole; 3. a first lens; 4. a second lens; 5. a converging lens; 6. a filter member; 7. a collection component; 8. a first mounting plate; 81. a first groove; 82. a second groove; 9. a thermostatic plate; 10. a second mounting plate; 101. a third groove; 102. a fourth groove; 11. a limiting plate; 12. a first lens; 13. a first filter wheel; 14. a mirror; 15. a second lens; 16. a second filter wheel; 100. a sample tube; 200. and (4) a sample plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Referring to fig. 1 to 9, an embodiment of the present disclosure provides an optical detection apparatus, including: the device comprises a light source assembly 1, a light homogenizing plate 2, a plurality of first lenses 3, a plurality of second lenses 4, a converging lens 5, a filtering component 6 and a collecting component 7, wherein the light source assembly 1 is used for emitting exciting light, the light source assembly 1 is arranged on at least one side of the light homogenizing plate 2, the exciting light is made to be incident into the light homogenizing plate 2, a sample tube 100 is arranged below the light homogenizing plate 2, the exciting light is made to be incident into the sample tube 100 through the light homogenizing plate 2, and a sample to be detected in the sample tube 100 is excited to emit emitting light; the first lens 3 is located between the light homogenizing plate 2 and the sample tube 100 and is arranged in one-to-one correspondence with the sample tube 100, the second lens 4 is located above the light homogenizing plate 2, the second lens 4 is arranged in one-to-one correspondence with the sample tube 100, the converging lens 5 is located above the second lenses 4 and is used for converging emitted light, and the emitted light penetrates through the filtering component 6 and then enters the collecting component 7. The light homogenizing plate 2 includes a first light barrier 21 and a second light barrier 22, the first light barrier 21 is provided with a plurality of first light holes 211, the second light barrier 22 is provided with a plurality of second light holes 221, a first reflective film 23 is disposed below the first light barrier 21, a second reflective film 24 is disposed above the second light barrier 22, the first reflective film 23 is provided with a plurality of light receiving holes 231, and the second reflective film 24 is provided with a plurality of light inlet holes 241, when the excitation light enters the light homogenizing plate 2, the excitation light can be reflected mutually by the first reflective film 23 and the second reflective film 24, and then sequentially enters the sample tube 100 through the light inlet holes 241, the second light holes 221 and the first lens 3 (the first lens 3 can converge the excitation light into the sample tube 100), the sample to be measured in the sample tube 100 excites the emission light, a part of the emission light passes through the first lens 3, the second light holes 221, the light inlet holes 241, the light receiving holes 231, the first light collecting holes 211, the second lens 4, the lens 5, the filtering component 6, the first lens 7, the second lens 7, the light collecting holes, the second lens 7, the light collecting parts, the light collecting holes 7, the second light collecting parts, the light collecting parts and the light collecting parts 2, and the light collecting parts 2. In the whole process, the excitation light is homogenized through the light homogenizing plate 2, so that the excitation light reaching each sample tube 100 is light energy or the incidence angle approaches to uniformity and consistency, and after the emission light is converged through the second lens 4 and the converging lens 5, the emission light is filtered out to obtain light with a required waveband through the filter part 6, and the light is emitted into the acquisition part 7 to be acquired and analyzed. Through the optical detection device of the application, make all sample tubes 100 can receive the exciting light simultaneously, and the exciting light received all approaches to evenly, unanimously, not only avoided a plurality of samples that await measuring among the prior art because of receiving the exciting light there is the result difference problem that the time interval arouses, on the other hand, still make the exciting light that arrives all sample tubes 100 approach to evenly, unanimously, thereby the difference of testing result has been avoided, the reliability of testing result has been improved, in addition still gather the analysis at same moment after all emission lights converge through convergent lens 5 and collecting element 7 (the process of gathering the analysis also is the testing process), the existence of the factor that probably influences the testing result has been avoided further, there is the time interval factor when the emission light that arouses to the different samples that await measuring gathers for example.

The first lens 3, the first light transmission hole 211, the second light transmission hole 221, the light receiving hole 231, the light inlet hole 241 and the second lens 4 are coaxially arranged, so that excitation light and emission light can be conveniently emitted into and out of the light homogenizing plate 2.

In addition, the aperture of the first light-transmitting hole 211 is smaller than or equal to the aperture of the light-receiving hole 231, the aperture of the light-receiving hole 231 is smaller than the aperture of the second light-transmitting hole 221, and the aperture of the second light-transmitting hole 221 is smaller than or equal to the aperture of the light-entering hole 241. Because the aperture of the light entrance hole 241 is larger than the aperture of the light receiving hole 231, the excitation light emitted upward by the second reflective film 24 is less, the probability that the excitation light enters the collecting component 7 through the light receiving hole 231 and is collected is also reduced, and thus the interference on the subsequent emission light collection result is also less, and meanwhile, because the aperture of the light entrance hole 241 is larger than the aperture of the light receiving hole 231, the excitation light on the first reflective film 23 can more easily reach the sample tube 100 through the light entrance hole 241 to excite the sample to be measured in the sample tube 100, in other words, the excitation light on the first reflective film 23 in the part of the region vertically corresponding to the first reflective film 23 from the edge of the light receiving hole 231 to the edge of the light entrance hole 241 can directly pass through the light entrance hole 241, and does not need to reach the sample tube 100 through the light entrance hole 241 after multiple reflections between the first reflective film 23 and the second reflective film 24. The aperture of the light receiving hole 231 is greater than or equal to the aperture of the first light transmitting hole 211, and at this time, the first light barrier 21 can completely block the light emitted from the first reflective film 23, so as to prevent the redundant light from entering the filter element 6, and prevent the light emitted from the first reflective film 23 from entering the sample tube 100 or entering the sample tube 100 less, thereby avoiding the interference with the detection result. The aperture of the light inlet 241 is greater than or equal to the aperture of the second light hole 221, and at this time, the second light blocking plate 22 can completely block the light emitted by the second reflective film 24, so as to prevent the excessive excitation light from irradiating the outer wall of the sample tube 100 and entering the sample tube 100, thereby easily controlling the excitation light entering the plurality of sample tubes 100 to be consistent.

The above-mentioned filtering means 6 may be an emission light filter for filtering the emission light to obtain light of a desired wavelength band in the emission light.

Since the emitted light is a light having a range of wavelength bands and different light energies of different wavelength bands, the wavelength band of the emitted light having the strongest light energy can be captured by the filter member 6.

The first light barrier 21 and the second light barrier 22 may be metal films, such as aluminum films, but the specific material is not limited here, and only the light barrier function is achieved.

In some embodiments, the first reflective film 23 comprises a diffuser film or a brightness enhancement film and the second reflective film 24 comprises a diffuser film or a brightness enhancement film.

The first reflection film 23 and the second reflection film 24 can be diffusion films, the excitation light and the emission light can be reflected between the two diffusion films, and the excitation light and the emission light can be diffused, so that the diffusion films can uniformly emit light integrally, and the light uniformizing effect is good.

The first reflective film 23 and the second reflective film 24 may be both brightness enhancement films, and the excitation light and the emission light may be reflected between the two brightness enhancement films, that is, the excitation light and the emission light may be reflected instantly when they reach the brightness enhancement films without loss.

The first reflective film 23 and the second reflective film 24 herein may also be diffusion films and brightness enhancement films, and the excitation light and the emission light can be reflected between the first reflective film 23 and the second reflective film 24, that is, the excitation light and the emission light first strike the diffusion films, most of the excitation light and the emission light will diffuse through the diffusion films, and a small part of the excitation light and the emission light may strike the brightness enhancement films and then reflect to another diffusion film, so the light uniformizing effect is also good.

The first reflective film 23 may be a diffusion film, the second reflective film 24 may be a brightness enhancement film, the excitation light and the emission light are reflected between the diffusion film and the brightness enhancement film, that is, the light can be diffused when the excitation light and the emission light are irradiated to the diffusion film, the diffusion film uniformly emits light as a whole, the excitation light and the emission light can be instantaneously reflected when the excitation light and the emission light are irradiated to the brightness enhancement film without loss, and the light uniformizing effect is also good because the excitation light and the emission light can be diffused and reflected for a plurality of times.

It should be noted that, when the excitation light is incident on the diffusion film, the diffusion film can emit light uniformly as a whole, so that the light can be blocked by the arrangement of the first light blocking plate 21, the excessive light is prevented from entering the filtering component 6 and the collecting component 7, and the light emitted by the diffusion film is also prevented from entering the sample tube 100 or entering the sample tube 100 less, so as to avoid interference with the detection result. The second light blocking plate 22 also functions to prevent excessive excitation light from being irradiated onto the outer wall of the sample tube 100 and entering the sample tube 100, so that it is easy to control the excitation light entering the plurality of sample tubes 100 to be uniform.

In some embodiments, the optical detection device further includes a first mounting plate 8, the first mounting plate 8 is located below the light homogenizing plate 2, the first mounting plate 8 is provided with a plurality of first grooves 81 and a plurality of second grooves 82 located below the first grooves 81, and the size of the first grooves 81 is smaller than that of the second grooves 82; a constant temperature plate 9 is arranged below the first mounting plate 8, a plurality of first through holes are formed in the constant temperature plate, and the first through holes and the first lenses 3 are arranged in a one-to-one correspondence manner; the first lens 3 is located between the second groove 82 and the first through hole. The installation of first lens 3 is not only made things convenient for, and the setting through thermostatic plate 9 can prevent moreover that the sample to be measured in sample tube 100 from forming the liquefaction when evaporating at high temperature and dripping and attaching to first lens 3, influences the light path of excitation light and emitted light, and the setting through the through-hole can not hinder excitation light and emitted light and incides into sample tube 100 simultaneously.

Here, the size of the first groove 81 is smaller than that of the second groove 82, and the first lens 3 is positioned between the second groove 82 and the first through hole, so that the upper side of the first lens 3 is limited by the first groove 81, and the lower side of the lens is limited by the first through hole, thereby fixing the first lens 3.

The O-ring for stabilizing the first lens 3 is provided in the second groove 82, and the O-ring surrounds the outer periphery of the first lens 3, thereby further stabilizing the first lens 3.

In some embodiments, the optical detection device further includes a second mounting plate 10, the second mounting plate 10 is located above the light homogenizing plate 2, the second mounting plate 10 is provided with a plurality of third grooves 101 and a plurality of fourth grooves 102 located above the third grooves 101, and the size of the third grooves 101 is smaller than that of the fourth grooves 102; a limiting plate 11 is arranged above the second mounting plate 10, the limiting plate 11 is provided with a plurality of second through holes, and the second through holes are arranged in one-to-one correspondence with the second lenses 4; the second lens 4 is located between the fourth groove 102 and the second through hole, so that the second lens 4 can be conveniently installed, the size of the third groove 101 is smaller than that of the fourth groove 102, the second lens 4 is located between the fourth groove 102 and the second through hole, therefore, the lower portion of the second lens 4 is limited by the third groove 101, and the upper portion of the second lens 4 is limited by the second through hole, so that the second lens 4 is fixed.

The fourth groove 102 is provided with an O-ring for stabilizing the second lens 4, and the O-ring surrounds the outer periphery of the second lens 4, thereby further stabilizing the second lens 4.

In some embodiments, when the collecting component 7 is configured as a shooting component, such as a camera, the optical detection apparatus further includes a first lens 12 and a first filter wheel 13 located above the first lens 12, the first filter wheel 13 includes at least one first accommodating space, the filter component 6 is located in the first accommodating space, the filter component 6 is coaxially disposed with the first lens 12 and the shooting component, the emitted light is incident into the shooting component through the first lens 12 and the filter component 6, and finally an image of a detection result of a sample to be detected at a position is shot through the shooting component, and the detection results of all samples to be detected can be collected at the same time through shooting component shooting, so as to complete optical detection of the sample to be detected in the sample tube 100; due to the adoption of the shooting type detection method of the shooting piece, the emitted light information of all samples to be detected can be simultaneously collected at the same time, so that the detection method is quicker, and the problem of detection result difference caused by the collection time interval is avoided. In addition, in this way, because the used components are all low in cost, the cost of the whole optical detection device is low.

The first filter wheel 13 is rotatable and supported by a bracket (not shown), and when the first filter wheel 13 is rotated, one of the filter members 6 is disposed coaxially with the first lens 12 and the photographic element. It should be noted that when there are a plurality of first accommodating spaces, different types of filter components 6 can be accommodated in different first accommodating spaces, for example, different types of emission light filters can be accommodated, and by rotating the first filter wheel 13, different filter components 6 can be switched to meet different optical detection requirements.

In some embodiments, when the collecting element 7 is configured as a hyperspectral meter, the optical detection device further includes a reflector 14, a second lens 15, and a second filter wheel 16 located on one side of the second lens 15, the second filter wheel 16 includes at least one second accommodating space, the filter element 6 is located in the second accommodating space, the filter element 6 is coaxially disposed with the second lens 15 and the hyperspectral meter, and the emitted light is reflected by the reflector 14 and enters the hyperspectral meter through the filter element 6 and the second lens 15. When the reflector 14 rotates to an angle or adjusts the position of the reflector 14, the emission light emitted from one row of the sample tubes 100 may be reflected to the filter unit 6 of the second filter wheel 16 for filtering, and then enters the second lens 15 after filtering, and the rest of the emission light may not be reflected to the second lens 15, and finally the respective full spectrum diagram of all the emission light from one row of the sample tubes 100 may be obtained through the hyperspectral instrument, and when the reflector 14 rotates to another angle again, the respective full spectrum diagram of all the emission light from another row of the sample tubes 100 may be obtained, and the angle of the reflector 14 may be continuously switched, and finally the respective full spectrum diagram of all the emission light from the used sample tubes 100 may be obtained, thereby the full spectrum detection of the emission light may be realized, and the detection result may be more accurate.

In some embodiments, the light source assembly 1 includes a substrate 1a, a rotating shaft 1b is disposed on the substrate 1a, a rotating disc 1c is rotatably connected to the rotating shaft 1b, a plurality of laser light sources 1d are distributed on the rotating disc 1c, and different laser light sources 1d can be aligned to the light homogenizing plate 2 one by driving the rotating disc 1c to rotate, so that switching of different laser light sources 1d is realized, and different detection requirements are met.

The light source assembly 1 may be provided with a plurality of rotating discs 1c, and the rotating discs 1c are uniformly arranged on the same horizontal plane.

In some embodiments, two light source assemblies 1 are provided, and the two light source assemblies 1 are symmetrically arranged on two sides of the light distribution plate 2, so that the excitation light emitted by the light distribution plate 2 is more uniform.

Of course, the light source assemblies 1 can also be arranged in a plurality, and the light source assemblies 1 are uniformly distributed on the periphery of the light homogenizing plate 2, so that the exciting light emitted by the light homogenizing plate 2 is more uniform.

In some embodiments, an excitation light filter is disposed on a side of the laser light source 1d facing the light uniformizing plate 2 for filtering the excitation light to obtain light of a desired wavelength band in the excitation light.

The working process of the optical detection device comprises the following steps:

excitation light emitted by the laser light source 1d firstly enters the light homogenizing plate 2 and is reflected in the light homogenizing plate 2 through the first reflecting film 23 and the second reflecting film 24, wherein the first reflecting film 23 is taken as a diffusion film, the second reflecting film 24 is taken as a brightness enhancement film as an example, the excitation light emitted by the diffusion film is collected through the first lens 3 and then enters the sample tube 100, a sample to be detected in the sample tube 100 is excited to emit light, and the emitted light is collected through the first lens 3, the second lens 4 and the collecting lens 5 and then enters the collecting component 7 to be detected by the collecting component 7, so that optical detection of the sample to be detected in the sample tube 100 is completed. The reflection process of the excitation light in the light homogenizing plate 2 includes that after the excitation light is incident into the light homogenizing plate 2, the diffusion film can uniformly emit light as a whole, the brightness enhancement film can reflect the excitation light, the light emitted by the diffusion film sequentially passes through the light inlet 241 and the second light inlet 221, is collected by the first lens 3 and then enters the sample tube 100, then a part of the emission light excited by the sample to be measured in the sample tube 100 enters the collecting component 7 through the first lens 3, the second light inlet 221, the light inlet 241, the light receiving hole 231, the first light inlet 211, the second lens 4, the collecting lens 5 and the filtering component 6, and the other part of the emission light enters the light homogenizing plate 2 through the first lens 3, the second light inlet 221 and the light inlet 241, is reflected by the first reflecting film 23 and the second reflecting film 24, and then enters the collecting component 7 through the light receiving hole 231, the first light inlet 211, the second lens 4, the collecting lens 5 and the filtering component 6, and is collected and analyzed by the collecting component 7. In the whole process, the excitation light is homogenized through the light homogenizing plate 2, so that the excitation light reaching each sample tube 100 is light energy or the incidence angle approaches to uniformity and consistency, and after the emission light is converged through the second lens 4 and the converging lens 5, the emission light is filtered out to obtain light with a required waveband through the filter part 6, and the light is emitted into the acquisition part 7 to be acquired and analyzed. Through the optical detection device of the application, make all sample tubes 100 can receive the exciting light simultaneously, and the exciting light received all approaches to evenly, unanimously, not only avoided a plurality of samples that await measuring among the prior art because of receiving the exciting light result difference problem that there is time interval to cause, on the other hand, still make the exciting light that arrives all sample tubes 100 approach to evenly, unanimously, thereby the difference of testing result has been avoided, the reliability of testing result has been improved, in addition still gather the analysis at same moment through collecting lens 5 and collecting element 7 with all emission lights after assembling, the existence of the factor that probably influences the testing result has further been avoided, there is time interval's factor when the emission light that arouses to the different samples that await measuring for example gathers. In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. An optical detection device for a PCR instrument, comprising:

a light source assembly for emitting excitation light;

the light source assembly is arranged on at least one side of the light homogenizing plate, so that the exciting light is emitted into the light homogenizing plate, a sample tube is arranged below the light homogenizing plate, the exciting light is emitted into the sample tube through the light homogenizing plate, and a sample to be detected in the sample tube is excited to emit light; the light homogenizing plate comprises a first light barrier and a second light barrier positioned below the first light barrier, the first light barrier is provided with a plurality of first light transmission holes, the second light barrier is provided with a plurality of second light transmission holes, a first reflecting film is arranged below the first light barrier, a second reflecting film is arranged above the second light barrier, the first reflecting film is provided with a plurality of light receiving holes, and the second reflecting film is provided with a plurality of light inlet holes;

the plurality of first lenses are positioned between the light homogenizing plate and the sample tube and are arranged in one-to-one correspondence with the sample tube;

the second lenses are positioned above the light homogenizing plate and are arranged in one-to-one correspondence with the sample tubes;

a condensing lens located above the plurality of second lenses for condensing the emitted light;

a filter member located above the converging lens for obtaining light of a desired wavelength band in the emitted light;

a collection member through which the emitted light passes to enter the collection member.

2. The optical detection device for a PCR instrument as claimed in claim 1, wherein the first lens, the first light-transmitting hole, the second light-transmitting hole, the light-receiving hole, the light-entering hole and the second lens are coaxially disposed.

3. The optical detection device for a PCR instrument of claim 1, wherein the aperture of the first light-transmitting hole is smaller than or equal to the aperture of the light-receiving hole, the aperture of the light-receiving hole is smaller than the aperture of the second light-transmitting hole, and the aperture of the second light-transmitting hole is smaller than or equal to the aperture of the light-entering hole.

4. The optical detection device for the PCR instrument as claimed in claim 1, further comprising a first mounting plate, the first mounting plate is located below the light homogenizing plate, the first mounting plate is provided with a plurality of first grooves and a plurality of second grooves located below the first grooves, and the first grooves are smaller than the second grooves; a constant temperature plate is arranged below the first mounting plate, a plurality of first through holes are formed in the constant temperature plate, and the first through holes and the first lenses are arranged in a one-to-one correspondence manner; the first lens is located between the second groove and the first through hole.

5. The optical detection device for the PCR instrument as claimed in claim 1, further comprising a second mounting plate, the second mounting plate being located above the light homogenizing plate, the second mounting plate being formed with a plurality of third grooves and a plurality of fourth grooves located above the third grooves, the third grooves being smaller in size than the fourth grooves; a limiting plate is arranged above the second mounting plate, the limiting plate is provided with a plurality of second through holes, and the second through holes and the second lenses are arranged in a one-to-one correspondence manner; the second lens is positioned between the fourth groove and the second through hole.

6. The optical detection device for a PCR instrument as claimed in claim 1, wherein when the capturing component is configured as a camera, the optical detection device further comprises a first lens and a first filter wheel located above the first lens, the first filter wheel comprises at least one first receiving space, the filter component is located in the first receiving space, the filter component is coaxially arranged with the first lens and the camera, and the emitting light is emitted into the camera through the first lens and the filter component.

7. The optical inspection device for a PCR instrument as claimed in claim 1, wherein when the collection member is configured as a hyperspectral instrument, the optical inspection device further comprises a reflector, a second lens and a second filter wheel located at one side of the second lens, the second filter wheel comprises at least one second receiving space, the filter member is located in the second receiving space, the filter member is coaxially arranged with the second lens and the hyperspectral instrument, and the emission light is reflected by the reflector and enters the hyperspectral instrument through the filter member and the lens.

8. The optical detection device for a PCR instrument as claimed in claim 1, wherein the first reflective film comprises a diffuser film or a brightness enhancement film, and the second reflective film comprises a diffuser film or a brightness enhancement film.

9. The optical detection device for the PCR instrument as claimed in claim 1, wherein the light source assembly includes a substrate, a rotating shaft is disposed on the substrate, a rotating disc is rotatably connected to the rotating shaft, and a plurality of laser light sources are distributed on the rotating disc.

10. The optical detection device for the PCR instrument as claimed in claim 1, wherein there are two light source assemblies, and the two light source assemblies are symmetrically disposed on two sides of the light uniformizing plate.

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