CN115926955B - Multi-component culture medium sterilization auxiliary mixing device - Google Patents
Multi-component culture medium sterilization auxiliary mixing device Download PDFInfo
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- CN115926955B CN115926955B CN202211731797.3A CN202211731797A CN115926955B CN 115926955 B CN115926955 B CN 115926955B CN 202211731797 A CN202211731797 A CN 202211731797A CN 115926955 B CN115926955 B CN 115926955B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a multi-component culture medium sterilization auxiliary mixing device which comprises at least two culture bottles, wherein each culture bottle comprises a three-way bottle cap connector, each bottle cap connector comprises a vertical first connector part and a second connector part which is communicated with the first connector part at an angle, the first connector part is communicated with the bottle body of the culture bottle, a lifting piston in interference fit with the inner wall of the first connector part is arranged in the first connector part, the piston is positioned below or above the joint of the second connector part and the first connector part in an interference fit manner, a rotary joint is connected onto the second connector part, and the two rotary joints on the two culture bottles are detachably connected through a connecting pipe. The invention realizes the separate sterilization of different inorganic culture medium components and the pollution-free direct mixing after sterilization.
Description
Technical Field
The invention relates to the technical field of microbial fermentation equipment, in particular to a multi-component culture medium sterilization auxiliary mixing device.
Background
The inorganic culture medium is an important culture medium for microbial fermentation culture, and has different chemical components for different microbial fermentation. In the sterilization process, if the inorganic culture medium is mixed and sterilized simultaneously, the components are easy to form precipitates due to interaction, so that the effect of the components in the culture medium is invalid, for example, magnesium ions are important metal ions in the inorganic culture medium, insoluble precipitates such as magnesium hydroxide are easy to generate by high-temperature reaction with other substances, and thus the magnesium ions cannot play a role of the magnesium ions as enzyme cofactors in the culture medium, and therefore, the magnesium-containing compounds generally need to be sterilized separately; phosphate is generally used as buffer solution, and separate sterilization is also necessary for convenient use; in addition, the components of the inorganic medium are liable to form toxic substances together at high temperatures, which are disadvantageous for production. In summary, for some specific inorganic medium components, separate sterilization is required during sterilization, and mixing is performed after cooling after sterilization is completed. However, in order to avoid pollution, the mixing needs to be performed in an ultra-clean bench, which is inconvenient to operate, and the mixing operation in the ultra-clean bench also has a certain pollution risk. Therefore, a device which can sterilize different inorganic culture medium components separately and directly mix the components after cooling after sterilization is finished is developed, and the device does not need to be operated in an ultra-clean bench, is convenient and fast, and avoids secondary pollution.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a multi-component culture medium sterilization auxiliary mixing device, which realizes separate sterilization of different inorganic culture medium components and pollution-free direct mixing after sterilization.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a supplementary mixing arrangement of multicomponent culture medium sterilization, includes two at least blake bottles, the blake bottle includes three way type bottle lid interface, and the bottle lid interface includes vertical first interface portion and the second interface portion of angled intercommunication with first interface portion, first interface portion and blake bottle's bottle intercommunication, be equipped with in the first interface portion with first interface portion inner wall interference fit's liftable piston, piston interference location is in the below or the top of second interface portion and first interface portion junction, be connected with rotary joint on the second interface portion, two on two blake bottles rotary joint can dismantle through the connecting pipe and be connected.
The fixed sleeve capable of being fixed at different heights on the outer wall of the upper part of the first interface part is arranged outside the first interface part, and the top of the fixed sleeve is fixedly connected with the piston through a connecting rod.
The fixed cover of first interface portion upper portion outer wall is equipped with upper and lower spaced screw joint cover and lower screw joint cover, fixed lantern ring shape inner wall is equipped with the internal thread and lower internal thread that the screw thread opposite direction goes up, go up internal thread and upper screw joint cover cooperation spiro union, lower internal thread and lower screw joint cover cooperation spiro union, go up internal thread and upper screw joint cover, lower internal thread and lower screw joint cover different time spiro union.
The annular inner wall of the fixed sleeve is protruded to form a convex ring between the upper internal thread and the lower internal thread; the lower internal thread is in threaded connection with the lower threaded sleeve, and the convex ring is abutted to the end face of the lower threaded sleeve; when the upper internal thread is in threaded connection with the upper threaded sleeve, the convex ring is abutted to the end face of the upper threaded sleeve.
The fixed sleeve comprises an upper sleeve part with an inverted U-shaped section and an annular lower sleeve part which is detachably connected with the lower end of the upper sleeve part, and the upper internal thread and the lower internal thread are arranged on the inner wall of the lower sleeve part.
The lower end surface of the upper sleeve part is vertically protruded to form clamping columns uniformly distributed on the circumference, and the clamping columns are inserted into the lower sleeve part in a clamping manner.
The connecting pipe comprises interface parts positioned at two ends, and the interface parts are in threaded connection with the rotary joint.
The auxiliary mixing device also comprises a clamping frame for clamping the culture bottle.
The beneficial effects of the invention are as follows: different culture medium components are added into an independent culture bottle for culture, sealing or communication of the culture bottle is realized by adjusting the position of a piston, the independent sealing culture state and the subsequent mixing requirement are ensured, and independent sterilization is facilitated; the culture bottles with different components are connected through the detachable connecting pipe, and the lifting piston can realize direct mixing, so that secondary pollution can be effectively avoided.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is a schematic diagram of the structure of the present invention during mixing;
fig. 4 is an enlarged view at B in fig. 3.
In the figure: the culture flask 1, the bottle cap 2, the bottle cap interface 3, the first interface 31, the second interface 32, the upper screw sleeve 33, the lower screw sleeve 34, the rotary joint 4, the piston 5, the fixed sleeve 6, the upper sleeve 61, the lower sleeve 62, the upper internal thread 63, the lower internal thread 64, the convex ring 65, the clamping post 66, the connecting rod 67, the connecting pipe 8, the interface 81 and the clamping frame 9.
Detailed Description
The invention is further described with reference to the drawings and detailed description which follow:
as shown in fig. 1-4, a multi-component culture medium sterilization auxiliary mixing device comprises at least two culture bottles 1, wherein the culture bottles 1 comprise a three-way bottle cap interface 3, the bottle cap interface 3 comprises a vertical first interface part 31 and a second interface part 32 which is communicated with the first interface part 31 at an angle, and the first interface part 31 is communicated with a bottle body of the culture bottle 1; the culture bottle 1 comprises a bottle body and a bottle cap 2, the bottle cap 2 comprises a bottle cap connector 3, and a first connector part 31 fixedly penetrates through a main cap body of the bottle cap 2 and stretches into the bottle body. The first interface part 31 and the second interface part 32 are arranged at an upward acute angle, namely the second interface part 32 is arranged upwards and outwards in an inclined manner, so that the culture solution can be conveniently added when the culture bottle 1 is placed in the normal position, and the subsequent culture solution can be conveniently poured out.
The first interface part 31 is internally provided with a liftable piston 5 in interference fit with the inner wall of the first interface part 31, and the piston 5 is positioned below or above the joint of the second interface part 32 and the first interface part 31 in an interference manner; when the piston 5 is positioned below the joint of the second interface part 32 and the first interface part 31, the piston 5 seals the lower port of the first interface part 31, and the culture solution in the culture bottle 1 is sealed in the bottle body and cannot be poured out; when the piston 5 is positioned above the junction between the second port 32 and the first port 31, the second port 32 and the bottle body form a communication channel, and the culture medium in the culture bottle 1 flows out through the first port 31 and the second port 32. The positioning arrangement of the piston 5 at different positions plays the role of a valve.
Specifically, the fixing sleeve 6 which can be fixed at different heights on the outer wall of the upper part of the first interface part 31 is arranged outside the first interface part 31, and the top of the fixing sleeve 6 is fixedly connected with the piston 5 through the connecting rod 67. Still further, the upper outer wall of the first interface 31 is fixedly sleeved with an upper screw 33 and a lower screw 34 which are spaced up and down, the annular inner wall of the fixed sleeve 6 is provided with an upper internal screw 63 and a lower internal screw 64 which are opposite in screw direction, the upper internal screw 63 is in screw connection with the upper screw 33, the lower internal screw 64 is in screw connection with the lower screw 34, and the upper internal screw 63 is in screw connection with the upper screw 33, and the lower internal screw 64 is not in screw connection with the lower screw 34. That is, when the lower female screw 64 is screw-coupled with the lower screw housing 34, the upper female screw 63 is positioned below the upper screw housing 33; when the upper internal thread 63 is screwed with the upper screw 33, the lower internal thread 64 is spaced apart from the lower screw 34, and the lower internal thread 64 is positioned below the lower screw 34 if the flask 1 is inverted.
The annular inner wall of the fixed sleeve 6 is protruded to form a convex ring 65 positioned between the upper inner thread 63 and the lower inner thread 64; when the lower internal thread 64 is in threaded connection with the lower threaded sleeve 34, the convex ring 65 is abutted to the end surface of the lower threaded sleeve 34; when the upper internal thread 63 is in threaded connection with the upper threaded sleeve 33, the convex ring 65 abuts against the end surface of the upper threaded sleeve 33. The function of the collar 65 is to limit the screwing of the fixing sleeve 6.
The fixing sleeve 6 comprises an upper sleeve part 61 with an inverted U-shaped cross section and an annular lower sleeve part 62 detachably connected with the lower end of the upper sleeve part 61, and an upper internal thread 63 and a lower internal thread 64 are arranged on the inner wall of the lower sleeve part 62. A collar 65 is also provided on the inner wall of the lower sleeve portion 62. The lower end surface of the upper sleeve part 61 is vertically protruded to form clamping columns 66 uniformly distributed on the circumference, and the clamping columns 66 are inserted into the lower sleeve part 62 in a clamping manner. When the fixing sleeve 6 is actually installed, the lower screw sleeve 34 is fixedly installed on the first interface part 31, then the upper sleeve part 61 is installed to the lower screw sleeve 34, then the upper screw sleeve 33 is fixedly installed to the upper end of the first interface part 31, and finally the lower sleeve part 62 is spliced on the upper sleeve part 61. Through the cooperation structure setting of card post 66 and lower cover portion 62, realized the cooperation installation of fixed cover 6 and upper spiro union cover 33, lower spiro union cover 34, can guarantee simultaneously that the screwing of whole fixed cover 6 is smooth and easy.
The second interface part 32 is connected with a rotary joint 4, and two rotary joints 4 on two culture bottles 1 are detachably connected through a connecting pipe 8. The connection pipe 8 includes an interface portion 81 at both ends, and the interface portion 81 is detachably connected to the rotary joint 4. The rotary joint 4 is arranged so that when the connecting pipe 8 is installed, the connecting pipe 8 can not be twisted by adjusting the rotary joint 4 after the connector 81 is screwed, and the smoothness of pouring out the culture solution in the subsequent culture flask 1 is ensured.
In actual operation, different culture flasks 1 are adopted to perform separate culture and sterilization treatment separately, the lower internal thread 64 is in threaded connection with the lower threaded sleeve 34, the piston 5 covers the culture flask 1 under the joint of the second joint part 32 and the first joint part 31, the culture flask 1 can be placed to pour out the lost culture solution, and pollution of external substances such as bacteria can be avoided.
When different culture components are required to be mixed, the connecting pipe 8 is connected with the two rotary joints 4 of any two culture bottles 1 through the connecting pipe 8 in advance in a sterilization process, the fixed sleeve 6 on the two culture bottles 1 is reversely screwed, the lower internal thread 64 is disconnected with the lower threaded sleeve 34, the upper internal thread 63 is in threaded connection with the upper threaded sleeve 33, the piston 5 is driven to move to the position above the joint of the second connecting part 32 and the first connecting part 31, the second connecting part 32 is communicated with the interior of the culture bottle 1, the culture solution in the culture bottle 1 is poured, the culture solution in one culture bottle 1 flows out through the second connecting part 32 along the connecting pipe 8 and flows into the other culture bottle 1, direct mixing is realized, the mixing operation can be carried out in an ultra clean bench, and secondary pollution is avoided. Even if a plurality of culture flasks 1 are mixed, mixing can be achieved by connecting two rotary joints 4 via a connecting pipe 8.
The auxiliary mixing device of the present invention further comprises a holding frame 9 for holding the culture flask 1. When the culture solution in the culture bottle 1 is poured, the inverted culture bottle 1 is fixed by the clamping frame 9, so that the operation is more convenient.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (6)
1. The utility model provides a supplementary mixing arrangement of multicomponent culture medium sterilization, includes two at least blake bottle (1), its characterized in that: the culture bottle (1) comprises a three-way bottle cap interface (3), the bottle cap interface (3) comprises a vertical first interface part (31) and a second interface part (32) which is communicated with the first interface part (31) at an angle, the first interface part (31) is communicated with the bottle body of the culture bottle (1), a lifting piston (5) which is in interference fit with the inner wall of the first interface part (31) is arranged in the first interface part (31), the piston (5) is positioned below or above the joint of the second interface part (32) and the first interface part (31) in an interference manner, a rotary joint (4) is connected to the second interface part (32), and the two rotary joints (4) on the two culture bottles (1) are detachably connected through a connecting pipe (8);
the fixed sleeve (6) which can be fixed at different heights on the outer wall of the upper part of the first interface part (31) is arranged outside the first interface part (31), and the top of the fixed sleeve (6) is fixedly connected with the piston (5) through the connecting rod (67); the fixed cover of upper portion outer wall of first interface portion (31) is equipped with upper and lower spaced spiral shell cover (33) and lower spiral shell cover (34), fixed cover (6) annular inner wall is equipped with internal thread (63) and lower internal thread (64) that the screw thread opposite in direction, upper internal thread (63) and upper spiral shell cover (33) cooperation spiro union, lower internal thread (64) and lower spiral shell cover (34) cooperation spiro union, upper internal thread (63) and upper spiral shell cover (33), lower internal thread (64) and lower spiral shell cover (34) different simultaneous spiro union.
2. A multicomponent culture medium sterilization auxiliary mixing device according to claim 1, wherein: the annular inner wall of the fixed sleeve (6) is protruded to form a convex ring (65) positioned between the upper internal thread (63) and the lower internal thread (64); the lower internal thread (64) is in threaded connection with the lower threaded sleeve (34), and the convex ring (65) is abutted to the end face of the lower threaded sleeve (34); when the upper internal thread (63) is in threaded connection with the upper threaded sleeve (33), the convex ring (65) is abutted to the end face of the upper threaded sleeve (33).
3. A multicomponent culture medium sterilization auxiliary mixing device according to claim 1, wherein: the fixing sleeve (6) comprises an upper sleeve part (61) with an inverted U-shaped cross section and an annular lower sleeve part (62) which is detachably connected with the lower end of the upper sleeve part (61), and the upper internal thread (63) and the lower internal thread (64) are arranged on the inner wall of the lower sleeve part (62).
4. A multicomponent culture medium sterilization aid mixing device according to claim 3, wherein: the lower end surface of the upper sleeve part (61) is vertically protruded to form clamping columns (66) uniformly distributed on the circumference, and the clamping columns (66) are inserted into the lower sleeve part (62) in a clamping mode.
5. A multicomponent culture medium sterilization auxiliary mixing device according to claim 1, wherein: the connecting pipe (8) comprises interface parts (81) positioned at two ends, and the interface parts (81) are in threaded connection with the rotary joint (4).
6. A multicomponent culture medium sterilization auxiliary mixing device according to claim 1, wherein: the auxiliary mixing device also comprises a clamping frame (9) for clamping the culture bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211731797.3A CN115926955B (en) | 2022-12-30 | 2022-12-30 | Multi-component culture medium sterilization auxiliary mixing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211731797.3A CN115926955B (en) | 2022-12-30 | 2022-12-30 | Multi-component culture medium sterilization auxiliary mixing device |
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| CN115926955A CN115926955A (en) | 2023-04-07 |
| CN115926955B true CN115926955B (en) | 2023-07-04 |
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| FR2946059B1 (en) * | 2009-06-02 | 2013-12-27 | Interlab | METHOD FOR SEEDING A CULTURE SUBSTRATE WITH AN AUTOMATE |
| CN202406587U (en) * | 2012-01-07 | 2012-09-05 | 李好勇 | Edible fungus liquid strain culture device |
| CN106416749B (en) * | 2016-08-31 | 2020-02-07 | 四川宝生药业发展有限公司 | Method for reducing operation pollution in liquid strain culture process |
| CN207418761U (en) * | 2017-11-08 | 2018-05-29 | 齐齐哈尔医学院 | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
| CN111763625A (en) * | 2020-06-02 | 2020-10-13 | 王甲银 | Clinical laboratory uses anti-pollution blake bottle |
| CN212925019U (en) * | 2020-08-18 | 2021-04-09 | 四川大学华西医院 | Bacteria culture device |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010268710A (en) * | 2009-05-20 | 2010-12-02 | Lg Support:Kk | Method for producing liquid spawn of mushroom, and cap of seed spawn-culturing bottle |
| CN209537509U (en) * | 2019-01-14 | 2019-10-25 | 武汉普诺赛生命科技有限公司 | A kind of high leakproofness 500mL plastic culture base square vase |
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