CN115903203A - Reading machine and oil dripping control method thereof - Google Patents

Abstract

The embodiment of the application provides a film reader, which comprises an objective table, an imaging device, an oil dripping device and a control device. The control device is connected with the oil dripping device and the imaging device in a communication mode and is configured to: determining an interested shooting area of a sample film on a sample slide, determining the oil dropping amount and/or the oil dropping position of mirror oil to be dropped according to the interested shooting area, and controlling an oil dropping device to drop the mirror oil to the sample slide according to the oil dropping amount and/or the oil dropping position. The slide reading machine can determine a proper oil dripping amount and/or oil dripping position according to the slide reading mode, and dripping the mirror oil to the sample slide according to the determined oil dripping amount and/or oil dripping position so as to control the mirror oil within the range of the sample slide. According to the scheme, not only can waste of consumables caused by redundant mirror oil be avoided, but also manual cleaning operation after mirror oil accumulation and influence on the performance of a film reading machine can be avoided. In addition, the embodiment of the application also provides an oil dripping control method of the film reader and a control method of the oil dripping device of the film reader.

Description

Reading machine and oil dripping control method thereof

Technical Field

The application relates to the technical field of medical equipment, in particular to a film reader and an oil dripping control method thereof.

Background

The slide reader, also called a cell morphology analyzer, is an instrument for analyzing cells or other components on a sample slide of blood (such as peripheral blood), bone marrow, body fluid, and the like. The main work flow is that the sample slide is shot based on the microscopic optical module and the digital shooting module, cells in the sample slide are examined and analyzed in morphology by using the sample image, and the examination and analysis result is provided for a user in a report form.

In order to enable a shot sample image to clearly reflect characteristics of cells, such as clear inner texture characteristics of cell nuclei, fineness degree of cytoplasm particles and the like in a film reading process, a high-numerical aperture microscopic optical objective lens can be used, but the objective lens approaches the optical resolution limit of a microscope, so that the optical aperture angle of the objective lens needs to be increased by means of immersion oil, and the high-definition imaging effect can be achieved.

At present, the manner of dripping the mirror oil into the slide reader is to drip the mirror oil into the sample slide quantitatively, but the redundant mirror oil is accumulated on an objective table or other parts, so that the labor is consumed for cleaning, and the normal operation of the slide reader is possibly influenced.

Disclosure of Invention

In view of this, the present application provides a film reading machine and an oil dripping control method thereof, so as to avoid the manual cleaning cost caused by the excessive mirror oil and the influence on the normal operation of the film reading machine.

In a first aspect, an embodiment of the present application provides a film reader, including:

a stage for carrying a sample slide to which a sample film is applied;

an imaging device including a plurality of objective lenses and a camera for taking an image of the sample film under the objective lenses;

the oil dripping device comprises an oil storage part, an oil dripping part and an oil pipe for connecting the oil storage part and the oil dripping part, wherein the oil dripping part is used for dripping mirror oil onto the sample glass;

a control device communicatively coupled to the oil dripping device and the imaging device and configured to: determining an interested shooting area of a sample film on the sample slide, determining the oil dripping amount and/or the oil dripping position of mirror oil to be dripped according to the interested shooting area, and controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position.

In a second aspect, an embodiment of the present application provides an oil dripping control method, which is applied to a reader, and the method includes:

loading a sample slide carrying a sample film to a stage of the reader;

determining an interested shooting area of a sample film on the sample slide, and determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped according to the interested shooting area;

and controlling an oil dripping device of the reader to drip mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position.

In a third aspect, an embodiment of the present application provides a control method for an oil dripping device of a slide reader, where the oil dripping device includes an oil dripping portion, a fixed displacement pump, and an oil storage portion, and the method includes:

determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped on the sample slide of the objective table;

determining the oil dripping times according to the oil dripping amount and the capacity of the quantitative pump;

controlling the oil dripping part and the objective table to perform relative motion so as to position the oil dripping part to the oil dripping position;

and controlling the dosing pump to suck the mirror oil from the oil storage part at least once according to the oil dripping times and driving the mirror oil to be output from the oil dripping part so that the mirror oil is dripped to the oil dripping position of the sample slide.

Therefore, according to the technical scheme, the proper oil dropping amount and/or oil dropping position can be determined according to the cell shooting mode, the mirror oil is dropped into the sample slide according to the determined oil dropping amount and/or oil dropping position, and the mirror oil is controlled within the range of the sample slide, so that the waste of consumables caused by redundant mirror oil can be avoided, and the manual cleaning operation after the mirror oil is accumulated and the influence on the performance of a slide reading machine can be avoided.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of a reader;

FIG. 2 is another schematic structural diagram of the reader;

FIG. 3 is a schematic structural diagram of an oil dripping device of the reader;

FIG. 4 is a schematic diagram of a control device of the CD reader;

FIG. 5 is a flow chart of a method for controlling oil dripping by a control device of the reader;

FIGS. 6A-6D are several schematic views of a sample film at a capture area of interest;

FIG. 7 is a schematic diagram of a method of determining the amount of oil dripping based on a shot region of interest;

fig. 8 is a schematic diagram of oil dripping at different oil dripping positions of a sample slide.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.

The slide reader is a device for analyzing cell morphology, and in order to obtain a clear image of cells during the slide reading process, a liquid substance with a refractive index larger than that of air, such as immersion oil or water, can be dropped between a sample slide and an objective lens of an imaging device, and the liquid substance can be called mirror oil.

Present reading machine is the mirror oil of drop ration at every turn, if the mirror oil of drop too little can lead to reading the piece effect worsen, nevertheless because mirror oil both is the consumptive material and is the pollutant, drop mirror oil if excessive not only increases the detection cost, and along with reading the long-term work of piece machine, can accumulate gradually at the objective table, influence reading the inside device of piece machine if set up optical device, light source etc. below the objective table, if the mirror oil becomes sticky or even dry and hard under the serious condition, can lead to reading the unable normal operating of piece machine. The conventional treatment measure for avoiding the accumulation of the mirror oil at present is to clean redundant mirror oil manually at regular intervals so as to clean residual mirror oil inside an instrument, but the manual cleaning mode can influence the use experience of a user on a reading machine.

Therefore, the embodiment of the application provides the film reading machine which can automatically and adaptively drop the mirror oil according to the actual film reading situation. Referring to fig. 1, fig. 2, and fig. 3, an image reader provided in an embodiment of the present application includes: the object stage 100, the imaging device 200, the oil dripping device 300 and the control device 400.

Specifically, the stage 100 is used to carry a sample slide 500 to which a sample film is applied.

Fig. 2 shows a schematic view of an imaging device 200 and a specimen slide 500. For example, the imaging device 200 includes a camera 201 and a lens group 202 for taking an image of a sample thin film applied by a sample slide 500. In one embodiment, the lens assembly 202 may include a first objective lens 2021 and a second objective lens 2022, and may further include a third objective lens 2023 and/or an adapter 2024, wherein the first objective lens may be a 10-fold objective lens, the second objective lens may be a 40-fold objective lens or a 100-fold objective lens, and of course, the lens assembly may include only one 40-fold objective lens or only one 100-fold objective lens. Or, the objective lens may include any one or combination of 4-time objective lens, 10-time objective lens, 40-time objective lens and 100-time objective lens, and of course, may also include 20-time objective lens and/or 60-time objective lens, and the specific arrangement of the objective lens may be selected according to actual situations. Illustratively, the low power objective lens includes a 4 power objective lens and/or a 10 power objective lens and/or a 40 power objective lens, and the high power objective lens includes a 100 power objective lens (i.e., a high power oil lens).

The oil dripping device 300 includes an oil storage portion, an oil dripping portion and an oil pipe for connecting the oil storage portion and the oil dripping portion, and the oil dripping portion is used for dripping mirror oil onto the sample slide 500. Referring to fig. 3, the oil dripping device 300 may include an oil storage portion 301, an oil pipe 302, an oil dripping portion 303, and a driving member 304. In one specific implementation, the oil dropping portion 303 may be specifically an oil dropping needle, which is mounted on the image forming apparatus 200. In a specific implementation manner, the driving member 304 is a driving pump such as a fixed displacement pump, the driving member 304 is connected with the oil storage portion 301 and the oil dripping portion 303 through an oil pipe 302, the driving member 304 is used for sucking the mirror oil in the oil storage portion 301, and the driving member 304 is further used for dripping the sucked mirror oil onto the sample slide through the oil dripping portion 303.

As shown in fig. 4, in a specific implementation, the control device 400 at least includes: a processing component 401, a RAM402, a ROM403, a communication interface 404, a memory 405, and an I/O interface 406, wherein the processing component 401, the RAM402, the ROM403, the communication interface 404, the memory 405, and the I/O interface 406 communicate over a bus 407. The control device 400 in this embodiment establishes communication with the imaging device 200 and the oil dripping device 300 through the communication interface 404, and can be in communication with a driving unit for driving the movement of the stage 100 or the sample slide 500.

The processing component may be a CPU, GPU or other chip with computing capabilities.

The memory 405 stores various computer programs such as an operating system and an application program to be executed by the processor module 401, and data necessary for executing the computer programs. In addition, data stored locally during the sample testing process, if desired, may be stored in memory 405.

The I/O interface 406 is constituted by a serial interface such as USB, IEEE1394, or RS-232C, a parallel interface such as SCSI, IDE, or IEEE1284, and an analog signal interface composed of a D/a converter and an a/D converter. The I/O interface 406 is connected to an input device including a keyboard, a mouse, a touch panel, or other control buttons, and the user can directly input data, such as the number of cells to be photographed in the photographing mode, to the control apparatus 400 using the input device. In addition, a display having a display function, such as: liquid crystal screen, touch screen, LED display screen, etc., and the control device 40 may output the processed data as image display data to a display for displaying, for example: analytical data, instrument operating parameters, etc.

The communication interface 404 is an interface that may be any communication protocol known today. The communication interface 404 communicates with the outside through a network. The control device 400 may communicate data with any device connected via the network via the communication interface 404 using a communication protocol.

Based on the partial structure examples of the film reader provided in fig. 1 to 4, the following describes the oil dropping control process of the control device in the film reader according to the embodiment of the present application. For convenience of understanding, the following examples are described with blood smears as sample slides and blood films as sample films.

The control device 400 in the reader establishes communication connection with the imaging device 200 and the oil dripping device 300 through the communication interface 404, and is configured to execute a control flow as shown in fig. 5, and the control flow specifically may include steps 501-503.

501: the interesting shooting area of the sample film on the sample slide is determined.

Specifically, the sample film may also be referred to as a sample coating, and the sample film is applied to the slide by smearing, spreading, or the like, thereby preparing the sample slide. In different film reading scenes, analysis requirements for samples in the sample film are different, and therefore, regions interested to be shot when the images of the sample film are shot may be different. Therefore, the interested shooting area of the sample film needs to be determined according to the reading requirement. One or more regions of interest may be used.

In one specific implementation, a shooting mode of the sample slide can be obtained to determine a shooting region of interest of the sample film according to the shooting mode. Specifically, before the film reader reads the film, the user may set a shooting mode (also referred to as a film reading mode) of the film reader, for example, the shooting mode includes but is not limited to: a designated area mode, a red blood cell capture mode (RBC mode), a white blood cell capture mode (WBC mode), a platelet capture mode (PLT mode), a custom mode, and any combination thereof, wherein the platelet capture mode can be further specifically subdivided into a platelet normal mode and a platelet enhancement mode. Of course, the shooting mode can also be automatically determined by the film reader according to the related information of the sample film.

The requirements of different shooting modes on the mirror oil may have larger difference due to the fact that the interested shooting areas of the different shooting modes are different, so that the interested shooting area of each shooting mode can be determined respectively, and then the oil dripping information can be determined according to the interested shooting areas.

For example, the region of interest can be determined by scanning the imaging device under a low-power objective. One way of implementing this is, for example, to look for a cell region satisfying the condition by scanning the sample smear under a 10-fold or 40-fold low-power objective lens, and the found cell region is determined as the photographing region of interest. Alternatively, the photographing region of interest is a target-designated region previously designated by a user or a device.

502: and determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped according to the interested shooting area.

The oil dropping information determined by the interested shooting area may specifically include an oil dropping amount and/or an oil dropping position. The oil dropping amount can be the total oil dropping amount or the single oil dropping amount, and it can be understood that the oil dropping frequency is required to be determined if the oil dropping amount is the single oil dropping amount. The oil dropping position can be one or more. The mirror oil dripped at the at least one oil dripping position according to the oil dripping amount needs to cover the interested shooting area.

How to determine the interested shooting area and the oil dripping information of the interested shooting area is described in detail below in conjunction with various shooting modes.

1. The region of interest in the designated region mode is a predetermined imaging region, and may be the entire region of the sample film or a partial region of the sample film. The photographing region of interest shown in fig. 6A is a square region including the entire sample film, the photographing region of interest shown in fig. 6B is a square region including the tail portion and the partial body portion of the sample film, and the photographing region of interest shown in fig. 6C is a partial square region within the body portion of the sample film, and illustratively, the region has a length of 10.6mm and a width of 8.4mm.

In a specific implementation, the photographing region of interest in this photographing mode may be a region in which an area and a position are previously designated, and for convenience of description, the region may be referred to as a target designation region. Since the area and position of the target specified region have been specified in advance, in this shooting mode, the imaging device performs image shooting after being positioned directly to the target specified region. In this case, the drip information (including the amount of drip and/or the drip position) corresponding to the target designated area may be stored in advance, and a specific example thereof may be stored in a memory inside the control device or a memory outside the control device. Further, the amount and/or position of oil dropping corresponding to the target designated area is obtained from the oil dropping information stored in advance.

In another specific implementation, a target region (a shooting region of interest) can be specified on the sample slide in advance, but the area and the size of the target specified region need to be automatically identified by the slide reader, that is, the imaging device needs to be controlled to scan the sample slide to obtain the area and the position of the target specified region. Further, the amount of oil dripping of the mirror oil is calculated from the area of the target designated area, and the position of oil dripping of the mirror oil is calculated from the position of the target designated area. The dropping position may be an optimal dropping position (the optimal dropping position is a position capable of covering the entire interested photographing region using the minimum dropping amount, such as a region middle position) and the amount of dropping oil at the optimal dropping position, or may be a plurality of dropping positions and the respective amounts of dropping oil at each dropping position.

2. If the sample film is a blood film and the shooting mode is a cell shooting mode such as a red blood cell shooting mode or a white blood cell shooting mode, the imaging device is controlled to scan and locate the target cells in the blood film so as to determine the distribution area (the interested shooting area) of the target cells. In the scanning and positioning process, the distribution area of the target cells is determined according to the overall distribution characteristics of the cells, such as the total number of the cells, the distribution overlapping performance or the uniformity, and the characteristics of the single cells, such as the morphological size, whether the single cells are malformed or not, whether the single cells are fragmented or not, and the like.

A specific scanning and positioning manner may be that a user may set the number of cells (e.g., 200 cells) as a scanning end condition before reading, an imaging device performs cell scanning on a blood smear under a low power microscope during reading, the scanning and positioning step may be stopped when cells meeting the number requirement and the cell shape requirement are scanned, the scanned cells may be referred to as target cells, and a region where the target cells are distributed is an interested shooting region. Further, the oil dropping amount and/or the oil dropping position of the mirror oil is determined according to the distribution area of the target cells.

3. And if the sample film is a blood film and the shooting mode is a platelet shooting mode, controlling the imaging device to position and scan the edge and/or tail area of the blood film, and determining the oil dripping amount and/or oil dripping position of the mirror oil according to the edge and/or tail area of the blood film. As shown in fig. 6D, the blood film includes a head portion, a body portion, and a tail portion in the smearing direction on the sample smear, and the regions of both side edges and the tail portion of the blood film may be determined as the photographing region of interest. Furthermore, the area and the position of the interested shooting area are located and scanned, the oil dripping amount of the mirror oil is determined according to the area of the area, and the oil dripping position is determined according to the position of the area.

A specific way of determining the oil dripping amount in any of the above shooting modes is to determine the number of shooting views corresponding to the shooting region of interest, and determine the oil dripping amount in the shooting region of interest according to a preset corresponding relationship between the shooting views and the oil dripping amount. For example, as shown in fig. 7, the shooting region of interest includes 19 shooting views, and 20ul of mirror oil is preset for each 20 shooting views, so that the oil dropping amount of the shooting region of interest can be determined to be 19/20ul. In the case of using the fixed displacement pump for oil dropping, if the capacity of the fixed displacement pump is 20ul, the fixed displacement pump may pump the mirror oil of one pump.

It should be noted that any two of the above imaging modes may be switched, for example, a red blood cell mode or a white blood cell mode to a platelet mode. If the shooting modes of the sample slide are switched and the oil dripping positions of the two shooting modes before and after switching are related, the oil dripping amount of the next shooting mode is reduced according to the oil dripping amount of the previous shooting mode, so that the waste of the mirror oil can be avoided, and the influence of the redundant mirror oil on the slide reader can be avoided.

As mentioned above, the shooting area of interest may be one or more, and the amount and/or position of oil dripping may be determined for each shooting area of interest by: determining the oil dripping amount corresponding to the interested shooting area based on the area of the interested shooting area, wherein the oil dripping amount comprises single dripping amount and dripping times; and determining at least one oil dripping position corresponding to the interested shooting area according to the position and the dripping times of the interested shooting area. After at least one oil dripping position is determined, the control device controls the oil dripping devices to move to at least one oil dripping position respectively, and the dosing pump is controlled at each oil dripping position to drip mirror oil to the sample slide once. It is understood that if the number of dropping times is more than 1, the dropping position is correspondingly more than 1. The single drop amount may be determined by the capacity of the oil supply unit, and for example, if the oil supply unit is a fixed displacement pump, the single drop amount is the capacity of the fixed displacement pump.

Different oil dripping modes can be set according to the number of dripping times:

in the first mode, the dropping times are more than 1, and the dropping is carried out at different positions for multiple times. Specifically, the oil dripping positions are evenly distributed according to the extending path and the oil dripping times of the interested shooting area. As shown in fig. 6D, assuming that the number of oil drops is 3, the interested photographing region is divided into four segments from the left side to the right side of the sample film according to the extending path of the interested photographing region, and the dividing point of each two segments is an oil drop position, thereby obtaining 3 oil drop positions. After a plurality of oil dripping positions are determined, the control device controls the oil dripping device to move to each oil dripping position respectively, and controls the quantitative pump to drip mirror oil to the sample slide once at each oil dripping position. In this embodiment, each oil drop is added at a different oil drop position. As shown in fig. 8, two oil dropping positions are determined in the interested shooting area, and after mirror oil is dropped once at each oil dropping position, the mirror oil can be mixed into the same oil film due to the flowing action.

In the second mode, if the dropping times are more than 1, the mirror oil is dropped at the same position for multiple times. Specifically, an optimal dropping oil position of the photographing region of interest, such as the center position of the photographing region of interest, may be determined, where the optimal dropping oil position drops the mirror oil a plurality of times according to the number of drops.

In the third mode, when the dripping times are equal to 1, the mirror oil is dripped at the optimal oil dripping position. Specifically, the above embodiment is suitable for a case where the area of the interested photographing region is large, that is, a large amount of the mirror oil needs to be dropped in the interested photographing region, and the mirror oil is dropped in a small amount and many times. If the oil dripping frequency determined by the small area of the interested shooting area is one time, an optimal oil dripping position such as a central position can be determined in the interested shooting area, and then the mirror oil is dripped at the optimal oil dripping position.

The above several ways can explain how the amount and/or position of oil dripping is determined for each shooting area of interest. If a plurality of shooting areas of interest are determined according to the shooting mode, for example, two areas are determined as the aggregation areas of white blood cells in the white blood cell shooting mode, both areas can be determined as the shooting areas of interest. At this time, the oil dropping amount and/or the oil dropping position of the plurality of photographing regions of interest may be determined in the following two ways. In the first method, the corresponding oil dropping amount and/or oil dropping position are determined for each interested shooting area, and the specific determination method may refer to the above description, which is not repeated herein. In the second way, an optimal oil dripping position and an oil dripping total amount dripped to the optimal oil dripping position are determined for a plurality of interested shooting areas (the oil dripping total amount is obtained by adding the oil dripping amount of each interested shooting area). And in the third mode, the oil dripping frequency is determined according to the area of an integral region formed by a plurality of interested shooting regions, the oil dripping position of each oil dripping is determined according to the position of the integral region, and oil dripping is respectively carried out at each oil dripping position. For a specific implementation of this method, reference may be made to the above description, which is not repeated herein.

503: and controlling the oil dripping device to drip mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position.

Specifically, the oil drip device can specifically include oil storage portion, oil drip portion and be used for connecting the oil pipe of oil storage portion and oil drip portion, and oil drip portion is like dripping the oil needle, and controlling means can control oil drip portion and drip the mirror oil on adding the sample glass.

In order to realize the control of the oil dripping position, in a specific implementation mode, the slide reader can further comprise a driving device which is in communication connection with the control device and is in mechanical connection with the oil dripping part and/or the object stage. In this case, the control device controls the driving device to drive the oil dropping portion and the stage to perform relative movement so as to position the oil dropping portion to the oil dropping position. The driving device may drive the oil dropping portion to move while the stage remains stationary, or drive the stage to move while the oil dropping portion remains stationary, or drive both the stage and the oil dropping portion to move, which is not limited in this application. And after the oil dripping part is positioned to the oil dripping position, the control device controls the oil dripping part to drip the mirror oil to the sample slide.

In order to realize the control of the oil dropping amount, in a specific implementation mode, the oil dropping device can further comprise an oil supply part which is in communication connection with the control device and is in mechanical connection with the oil dropping part. In this case, the control device controls the oil supply section to drive the mirror oil of the oil dropping amount to be output from the oil dropping section so that the mirror oil is dropped to the sample slide. The oil supply section can be understood as a drive means for sucking the scope oil from the oil reservoir section and pushing the scope oil out of the oil drip section. One way of realizing the oil supply unit is a fixed displacement pump, and the amount of the mirror oil sucked by the fixed displacement pump each time is fixed. Alternatively, the oil supply unit may be implemented as a variable pump in other ways, and in this case, the oil supply unit sucks the scope oil from the oil reservoir in response to a start signal from the control device and stops sucking the scope oil in response to a stop signal. Alternatively, the oil supply unit may be a valve pump having a valve, and the amount of the mirror oil sucked from the oil reservoir may be the determined amount of the dropped oil by controlling the on/off time of the valve. Alternatively, the oil supply unit may be a syringe, and in the case of a syringe, the mirror oil of the entire amount of oil dropped may be drawn from the oil reservoir at one time, or the mirror oil of a part of the amount of oil dropped may be drawn from the oil reservoir several times.

If the oil supply part is a fixed displacement pump, after the oil dripping amount of the interested region is calculated according to the interested shooting region, the oil dripping frequency is determined according to the oil dripping amount and the capacity of the fixed displacement pump, and the fixed displacement pump is controlled to suck the mirror oil from the oil storage part at least once according to the oil dripping frequency and drive the mirror oil to be output from the oil dripping part. For example, if the amount of oil dropped is 35ul and the capacity of the fixed displacement pump is 20ul, the number of oil drops can be determined to be 35/20, and since the amount of mirror oil pumped by the fixed displacement pump per time is fixed, the number of oil drops can be determined to be 2 after rounding up.

A concrete implementation mode of the oil dripping part is an oil dripping needle, oil is dripped on the sample glass sheet through the oil dripping needle, an objective table or the sample glass sheet can be shaken in a small range for spreading the mirror oil, or oil can be dripped on the sample glass sheet by dragging through the objective lens. Of course, the mirror oil can be spread by spreading in a dripping manner, specifically, the oil dripping device comprises a spreading part which can replace the oil dripping part or a part arranged outside an output port of the oil dripping part, the spreading part is similar to an oil brush, the control device controls the spreading part to spread the mirror oil with the oil dripping amount output from the oil dripping part to the sample glass slide, and the spread mirror oil forms an oil film by utilizing the adsorption effect of the mirror oil.

According to the technical scheme, the slide reader provided by the embodiment of the application can determine the appropriate oil dripping amount and/or oil dripping position according to the slide reading mode, and dripping the mirror oil on the sample slide according to the determined oil dripping amount and/or oil dripping position so as to control the mirror oil in the range of the sample slide. According to the scheme, not only can waste of consumables caused by redundant mirror oil be avoided, but also manual cleaning operation after mirror oil accumulation and influence on the performance of a film reading machine can be avoided. After the slide reading is finished, the mirror oil can be output to the slide reader along with the sample slide or conveyed to a storage device of the slide reader, so that the mirror oil is prevented from remaining in the sample slide.

In practical application, the mirror oil dripped to the sample slide may not meet preset conditions, such as insufficient oil dripping amount, insufficient extension of the mirror oil of the sample slide and the like, and the shot image is not clear enough due to insufficient mirror oil. In order to avoid this problem, the control device may control the oil dropping device to drop the mirror oil again to the sample slide according to the oil dropping amount and/or the oil dropping position when it is detected that the mirror oil dropped to the sample slide does not meet the preset condition. Specifically, the control device can detect the image quality of the shot image, such as parameters of definition, brightness, contrast and the like, and when the image quality is found not to meet the preset conditions, the film reading process is suspended and the oil dripping device is controlled to drip oil again.

Corresponding to a reader, the embodiment of the application also provides an oil dripping control method of the reader, which specifically comprises the following steps: loading the sample slide carrying the sample film to a stage of a slide reader; determining an interested shooting area of a sample film on a sample slide, and determining the oil dripping amount and/or the oil dripping position of mirror oil to be dripped according to the interested shooting area; and (3) controlling an oil dripping device of the reading machine to drip mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position. It should be noted that, the control method may refer to the above description, and is not described herein again. The oil dropping control method may be executed by the control apparatus described above, such as the control apparatus shown in fig. 4.

Under the condition that the film reader is provided with the metering pump, the embodiment of the application also provides a control method of the oil dripping device of the film reader. Specifically, the oil dripping device comprises an oil dripping part, a fixed displacement pump and an oil storage part, and the method comprises the following steps: determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped on the sample slide of the objective table; determining the oil dripping frequency according to the oil dripping quantity and the capacity of the quantitative pump; controlling the oil dripping part and the objective table to move relatively so as to position the oil dripping part to an oil dripping position; and controlling the quantitative pump to suck the mirror oil from the oil storage part at least once according to the oil dripping times and driving the mirror oil to be output from the oil dripping part so that the mirror oil is dripped to the oil dripping position of the sample slide. It should be noted that, the control method may refer to the above description, and is not described herein again. The oil dropping control method may be executed by the control apparatus described above, such as the control apparatus shown in fig. 4.

In the above description of the disclosed embodiments, features described in various embodiments in this specification can be substituted for or combined with each other to enable those skilled in the art to make or use the present application. Reference is made herein to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope hereof. For example, the various operational steps, as well as the components for performing the operational steps, may be implemented in differing ways depending upon the particular application or consideration of any number of cost functions associated with the operation of the system (e.g., one or more steps may be deleted, modified, or combined with other steps).

The terms "first," "second," and the like in the description and claims herein and in the above-described drawings are used for distinguishing between different objects and not necessarily for describing a particular sequential order. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements but may alternatively include other steps or elements not expressly listed or inherent to such process, method, or apparatus.

Additionally, as will be appreciated by one skilled in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium, which is pre-loaded with computer readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-ROMs, DVDs, blu Ray disks, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

The foregoing detailed description has been described with reference to various embodiments. However, one of ordinary skill in the art would recognize that various modifications and changes can be made without departing from the scope of the present disclosure. Accordingly, the disclosure is to be considered in an illustrative and not a restrictive sense, and all such modifications are intended to be included within the scope thereof. Also, advantages, other advantages, and solutions to problems have been described above with regard to various embodiments. However, the benefits, advantages, solutions to problems, and any element(s) that may cause any element(s) to occur or become more pronounced are not to be construed as a critical, required, or essential feature or element of any or all the claims. As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, system, article, or apparatus. Furthermore, the term "coupled," and any other variation thereof, as used herein, refers to a physical connection, an electrical connection, a magnetic connection, an optical connection, a communicative connection, a functional connection, and/or any other connection.

The above examples only show several embodiments, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent should be subject to the appended claims.

Claims (18)

1. A slide reader, comprising:

a stage for carrying a sample slide to which a sample film is applied;

an imaging device including a plurality of objective lenses and a camera for taking an image of the sample film under the objective lenses;

the oil dripping device comprises an oil storage part, an oil dripping part and an oil pipe for connecting the oil storage part and the oil dripping part, wherein the oil dripping part is used for dripping mirror oil onto the sample glass;

a control device communicatively coupled to the oil dripping device and the imaging device and configured to: determining an interested shooting area of a sample film on the sample slide, determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped according to the interested shooting area, and controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position.

2. The reader of claim 1,

the control device is specifically configured to: obtaining a shooting mode of the sample slide, and determining the interested shooting area according to the shooting mode.

3. The slide reader according to claim 2, wherein said control device is specifically configured to: and if the shooting mode is a designated area mode, acquiring the oil dripping amount and/or the oil dripping position corresponding to the target designated area from the pre-stored oil dripping information according to the target designated area of the sample slide.

4. The slide reader according to claim 2, wherein said control device is specifically configured to: and if the shooting mode is a specified region mode, controlling the imaging device to scan to obtain the area and the position of a target specified region corresponding to the specified region mode, calculating the oil dripping amount of the mirror oil according to the area of the target specified region, and calculating the oil dripping position of the mirror oil according to the position of the target specified region.

5. The machine according to claim 2, characterized in that the sample membrane is a blood membrane, and the control device is configured in particular to: and if the shooting mode is a cell shooting mode, controlling the imaging device to scan and position the target cells in the blood membrane so as to determine the distribution area of the target cells, and determining the oil dripping amount and/or the oil dripping position of the mirror oil according to the distribution area of the target cells.

6. The slide reader according to claim 2, wherein the sample film is a blood film, and the blood film sequentially comprises a head part, a body part and a tail part on the sample smear along the smearing direction;

the control device is specifically configured to: and if the shooting mode is a platelet shooting mode, controlling the imaging device to position and scan the edge and/or tail area of the blood membrane, and determining the oil dripping amount and/or oil dripping position of the mirror oil according to the edge and/or tail area of the blood membrane.

7. The slide reader according to claim 2, wherein the control device is further configured to: and if the shooting modes of the sample slide are switched and the oil dripping positions of the two shooting modes before and after switching are related, reducing the oil dripping amount of the next shooting mode according to the oil dripping amount of the previous shooting mode.

8. The slide reader according to claim 1, further comprising a driving device, wherein the driving device is connected with the control device in communication and mechanically connected with the oil dripping part and/or the stage;

when the step of controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping position is executed, the control device is specifically configured to:

and controlling the driving device to drive the oil dripping part and the objective table to move relatively so as to position the oil dripping part to the oil dripping position, and controlling the oil dripping part to drip mirror oil to the sample slide after the oil dripping part is positioned to the oil dripping position.

9. The slide reader according to claim 1, wherein the oil dripping device further comprises an oil supply part, the oil supply part is in communication connection with the control device and is in mechanical connection with the oil dripping part;

when the step of controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping amount is executed, the control device is specifically configured to:

and controlling the oil supply part to drive the mirror oil of the oil dripping amount to be output from the oil dripping part so that the mirror oil is dripped to the sample slide.

10. The slide reader of claim 9, wherein the oil supply is a fixed displacement pump; when the step of controlling the oil supply unit to drive the mirror oil of the oil dropping amount to be output from the oil dropping unit is executed, the control device is specifically configured to:

and determining the oil dripping frequency according to the oil dripping amount and the capacity of the fixed displacement pump, controlling the fixed displacement pump to suck the mirror oil from the oil storage part at least once according to the oil dripping frequency and driving the mirror oil to be output from the oil dripping part.

11. The slide reader according to claim 1, wherein the oil dripping device further comprises an applying part, and the applying part is mechanically connected with the oil dripping part;

when the step of controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping amount is executed, the control device is specifically configured to:

and controlling the coating part to coat the mirror oil of the oil dripping amount output from the oil dripping part on the sample glass slide.

12. The slide reader according to claim 1, wherein when the step of determining the oil dropping amount and/or the oil dropping position to which the mirror oil is to be dropped according to the shooting area of interest is performed, the control device is specifically configured to:

if the number of the interested shooting areas is multiple, determining the corresponding oil dripping amount and/or oil dripping position for each interested shooting area respectively; or the like, or a combination thereof,

if the interested shooting areas are multiple, determining an optimal oil dripping position and the total oil dripping amount dripped to the optimal oil dripping position for the interested shooting areas.

13. The slide reader according to claim 1, wherein when the step of determining the oil dropping amount and/or the oil dropping position to which the mirror oil is to be dropped according to the shooting area of interest is performed, the control device is specifically configured to:

determining the oil dropping amount corresponding to the interested shooting area based on the area of the interested shooting area, wherein the oil dropping amount comprises single dropping amount and dropping times;

and determining at least one oil dripping position corresponding to the interested shooting area according to the position of the interested shooting area and the dripping times.

14. The slide reader according to claim 13, wherein the oil dripping device further comprises a fixed displacement pump, and the single dripping amount is the capacity of the fixed displacement pump;

when the step of controlling the oil dripping device to drip the mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position is executed, the control device is specifically configured to:

and controlling the oil dripping device to move to the at least one oil dripping position respectively, and controlling the quantitative pump to drip the mirror oil once to the sample slide at each oil dripping position.

15. The slide reader according to claim 1, wherein the control device is further configured to control the oil dripping device to drip the mirror oil to the sample slide again according to the oil dripping amount and/or the oil dripping position if it is monitored that the mirror oil dripped to the sample slide does not meet preset conditions.

16. The slide reader according to claim 15, wherein the control device is further configured to control the imaging device to perform image capturing on the sample slide to which the mirror oil is dripped to obtain at least one sample image; and determining whether the mirror oil dripped to the sample slide meets a preset condition or not according to whether the image quality of the at least one sample image meets the preset condition or not.

17. An oil dripping control method is characterized by being applied to a reader, and the method comprises the following steps:

loading a sample slide bearing a sample film to a stage of the reader;

determining an interested shooting area of a sample film on the sample slide, and determining the oil dripping amount and/or the oil dripping position of the mirror oil to be dripped according to the interested shooting area;

and controlling an oil dripping device of the reader to drip mirror oil to the sample slide according to the oil dripping amount and/or the oil dripping position.

18. A control method of an oil dripping device of a slide reader is characterized in that the oil dripping device comprises an oil dripping part, a fixed displacement pump and an oil storage part, and the method comprises the following steps:

determining the oil dripping amount and/or the oil dripping position of mirror oil to be dripped on a sample slide of an objective table;

determining the oil dripping frequency according to the oil dripping quantity and the capacity of the quantitative pump;

controlling the oil dripping part and the objective table to perform relative motion so as to position the oil dripping part to the oil dripping position;

control the constant delivery pump follow the oil drip number of times at least once from the oil storage portion absorbs mirror oil and drive mirror oil is followed the oil drip portion is exported, so that the mirror oil drips to the sample slide the oil drip position.

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