CN115893679A - Nutrient matrix sustained-release medicament for stimulating bioremediation of VCHs polluted underground water - Google Patents
Nutrient matrix sustained-release medicament for stimulating bioremediation of VCHs polluted underground water Download PDFInfo
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- CN115893679A CN115893679A CN202211431052.5A CN202211431052A CN115893679A CN 115893679 A CN115893679 A CN 115893679A CN 202211431052 A CN202211431052 A CN 202211431052A CN 115893679 A CN115893679 A CN 115893679A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 44
- 239000003814 drug Substances 0.000 title claims abstract description 24
- 238000013268 sustained release Methods 0.000 title claims abstract description 23
- 239000012730 sustained-release form Substances 0.000 title claims abstract description 23
- 230000004936 stimulating effect Effects 0.000 title claims abstract description 14
- 239000011159 matrix material Substances 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 19
- 239000000758 substrate Substances 0.000 claims abstract description 17
- 239000011248 coating agent Substances 0.000 claims abstract description 8
- 238000000576 coating method Methods 0.000 claims abstract description 8
- 125000000896 monocarboxylic acid group Chemical group 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000003673 groundwater Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000007790 solid phase Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 238000009210 therapy by ultrasound Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 abstract description 20
- 238000006731 degradation reaction Methods 0.000 abstract description 20
- 244000005700 microbiome Species 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 8
- 239000003344 environmental pollutant Substances 0.000 abstract description 4
- 231100000719 pollutant Toxicity 0.000 abstract description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 28
- MVPPADPHJFYWMZ-UHFFFAOYSA-N chlorobenzene Chemical compound ClC1=CC=CC=C1 MVPPADPHJFYWMZ-UHFFFAOYSA-N 0.000 description 18
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical group ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 12
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 9
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 8
- 229960001701 chloroform Drugs 0.000 description 8
- 239000004576 sand Substances 0.000 description 8
- 241000192142 Proteobacteria Species 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 238000005070 sampling Methods 0.000 description 5
- 241000606125 Bacteroides Species 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 239000002957 persistent organic pollutant Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- 241000605059 Bacteroidetes Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- XSKZXGDFSCCXQX-UHFFFAOYSA-N thiencarbazone-methyl Chemical compound COC(=O)C1=CSC(C)=C1S(=O)(=O)NC(=O)N1C(=O)N(C)C(OC)=N1 XSKZXGDFSCCXQX-UHFFFAOYSA-N 0.000 description 2
- 241000606750 Actinobacillus Species 0.000 description 1
- 241001156739 Actinobacteria <phylum> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001265526 Gemmatimonadetes <phylum> Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- -1 and particularly Polymers 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted underground water, and relates to the technical field of water treatment. The invention uses wall material to coat the nutrient substrate to obtain the nutrient substrate sustained-release medicament with high coating rate and good sustained-release effect, wherein the nutrient substrate is NH 4 Cl0.5-1.5 parts, KNO 3 40 to 60 portions of Na 2 HPO 4 80 to 120 portions of CaCl 2 5-15 parts of MgSO (MgSO) 4 400-550 parts of MnSO 4 0.05 to 0.2 portion of ZnCl 2 0.5-2 parts of, CH 3 0.5-2 parts of COOH. The nutrient matrix sustained-release medicament can stimulate indigenous microorganisms in the underground water polluted by VCHs, and improve the abundance of the microorganisms, so that the degradation efficiency and the removal rate of the VCHs pollutants in the underground water are improved.
Description
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted underground water.
Background
The in-situ microbial remediation technology is to utilize artificial enrichment culture of indigenous microbial inoculum or exogenous microbial inoculum to degrade organic pollutants in underground water in the original underground water environment. Mainly according to the groundwater environment of a polluted site, the degradation effect of microorganisms is improved by stimulating the bacterial load of indigenous functional microorganisms, reaction substrates (nutrient substances and electron acceptors/donors) and the like by combining the physicochemical characteristics of organic pollutants, and the degradation removal of the organic pollutants is promoted.
VCHs (Volatile chlorinated hydrocarbons) are also main pollutants polluted by underground water, the problems of low abundance, low activity, long repair period and the like of indigenous functional microorganisms exist in the existing repair of the underground water polluted by the VCHs, and meanwhile, the added exogenous microbial inoculum is difficult to adapt to the low-temperature and anoxic underground water environment, so that how to efficiently repair the underground water polluted by the VCHs is a hot problem in the current industrial research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted underground water. The nutrient matrix sustained-release medicament can stimulate indigenous microorganisms in the underground water polluted by VCHs, improve the abundance of the microorganisms, and improve the proportions of Proteobacteria, firmicutes, bacteriodes, actinobacillus, chlamydiat and the like in the flora, thereby improving the degradation efficiency and removal rate of the VCHs pollutants in the underground water.
The invention is realized by adopting the following technical scheme:
a nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted groundwater comprises the following steps:
s1, preparing a nutrient medium
The nutrient medium comprises the following components in parts by weight:
NH 4 cl 1.0 part, KNO 3 50.0 parts of Na 2 HPO 4 100.0 parts of CaCl 2 10.0 parts of L, MgSO 4 500 parts of MnSO 4 0.1 part of ZnCl 2 1.0 part, CH 3 1.0 part of COOH;
s2, coating treatment
(1) Mixing the wall material and the nutrient medium, putting the mixture into water, and uniformly stirring the mixture at the temperature of 45-55 ℃ to obtain a mixed solution; the mass ratio of the wall material to the nutrient substrate to the water is 3-5;
(2) Putting ethanol into a container and placing the container in an ultrasonic instrument for ultrasonic treatment; cooling the mixed solution to room temperature, then dropwise adding the mixed solution into the container filled with the ethanol, and continuing ultrasonic treatment to obtain a suspension;
(3) Centrifuging the suspension to obtain a solid-phase material;
(4) And finally, washing and drying the solid phase material to obtain the nutrient matrix sustained-release medicament.
Preferably, the wall material of the present invention is starch, and particularly, potato starch is preferred.
Preferably, the dropping speed of the step (2) is 5-15mL/min.
Preferably, the centrifugal speed in the step (3) is 5000-8000rpm, and the centrifugal time is 10-15 min.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted underground water, which is smooth and hemispherical and is easy to migrate to a polluted area to stimulate the degradation of indigenous microorganisms; the sustained-release medicament has higher coating rate and excellent sustained-release performance.
The nutrient matrix sustained-release medicament can efficiently degrade VCHs (containing dichloroethylene, dichloromethane, dichloroethane, trichloromethane and chlorobenzene) pollutants in underground water, and the degradation rate is up to more than 98 percent after 7 days of action.
Detailed Description
The present invention will be described in detail with reference to the following embodiments in order to make the objects, features and advantages thereof more comprehensible. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
A nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted groundwater is prepared by the following steps:
s1, preparing a nutrient medium
The nutrient medium comprises the following components in parts by weight:
NH 4 cl 1.0 part, KNO 3 50.0 parts of Na 2 HPO 4 100.0 parts of CaCl 2 10.0 parts of L, mgSO 4 500 parts of MnSO 4 0.1 part of ZnCl 2 1.0 part, CH 3 1.0 part of COOH;
s2, coating treatment
(1) Mixing the wall material and the nutrient medium, putting the mixture into water, and stirring the mixture for 30min under the magnetic stirring of 300rpm at the temperature of 50 ℃ to obtain a mixed solution; the mass ratio of the wall material to the nutrient substrate to the water is 5;
(2) Putting ethanol into a container and placing the container in an ultrasonic instrument for ultrasonic treatment; cooling the mixed solution to room temperature, then dropwise adding (at a dropwise adding speed of 10 mL/min) the mixed solution into the container filled with the ethanol, and continuing ultrasonic treatment to obtain a suspension;
(3) Centrifuging the suspension (the centrifugation speed is 8000rpm, and the centrifugation time is 10 min.) to obtain a solid-phase material;
(4) And finally, washing and drying the solid phase material to obtain the nutrient matrix sustained-release medicament, wherein the coating rate of the nutrient matrix is 77.22%.
1g of the sustained-release agent prepared in example 1 was injected into 1L of underground water (water temperature: 10 ℃) contaminated with VCHs, wherein the underground water contained dichloroethylene, dichloromethane, chloroform, dichloroethane, chlorobenzene, and the concentrations of the above components were 200. Mu.g/L. After running for 10 days, sampling is carried out periodically to detect the degradation rate of each component of VCHs in the underground water and the gene detection (DNA content and flora composition) of indigenous microbial flora.
Test results show that the degradation effect of VCHs is obviously increased along with the change of time after the sustained-release medicament is added, and the degradation rates of dichloroethylene, dichloromethane, trichloromethane, dichloroethane and chlorobenzene in underground water after the operation for 3 days are 87.37%, 83.75%, 90.00%, 85.57% and 90.01% respectively. After the running is continued for 7 days, the degradation rate of various VCHs components in the underground water reaches over 98 percent. Compared with a comparative example (without any injection of medicament), the amount of indigenous microorganisms in the groundwater increased from 11.20ng/μ L to 18.50ng/μ L, and the abundance of Proteobacteria, actinobacteria, bacteroides and Gemmatimonadetes increased by 22.66% to 25.88%.
Example 2
A nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted underground water comprises the following preparation steps:
s1, preparing a nutrient medium
The nutrient medium comprises the following components in parts by weight:
NH 4 cl0.5 part, KNO 3 60 portions of Na 2 HPO 4 85 portions of CaCl 2 15 parts of L, mgSO 4 550 parts of MnSO 4 0.15 part of ZnCl 2 1.5 parts of, CH 3 0.8 part of COOH;
s2, coating treatment
(1) Mixing the wall material and the nutrient medium, putting the mixture into water, and stirring the mixture for 30min under the magnetic stirring of 300rpm at the temperature of 55 ℃ to obtain a mixed solution; the mass ratio of the wall material to the nutrient medium to the water is 5:1:100, respectively;
(2) Putting ethanol into a container and placing the container in an ultrasonic instrument for ultrasonic treatment; cooling the mixed solution to room temperature, then dropwise adding (at a dropping speed of 10 mL/min) the mixed solution into the container filled with the ethanol, and continuing ultrasonic treatment to obtain a suspension;
(3) Centrifuging the suspension (the centrifugal speed is 7000rpm, and the centrifugal time is 15 min.) to obtain a solid-phase material;
(4) And finally, washing and drying the solid phase material to obtain the nutrient matrix sustained-release medicament, wherein the coating rate of the nutrient matrix is 78.15%.
The slow release agent is tested according to the method of the embodiment 1, and the degradation rates of the dichloroethylene, the dichloromethane, the trichloromethane, the dichloroethane and the chlorobenzene in the underground water after the operation for 3d are 86.12%, 84.32%, 89.34%, 84.09% and 92.12% respectively. After the running is continued for 7 days, the degradation rate of various VCHs components in the underground water reaches over 98 percent. The amount of indigenous microbes in the groundwater increased from 12.10 ng/. Mu.L to 19.70 ng/. Mu.L, and the abundance of Proteobacteria, actinobacilla, bacteroides, and Gemmatimadides increased by 14.07% -28.26%.
The sustained-release agent prepared in example 2 was injected into underground water (water temperature 10 ℃) contaminated with VCHs, wherein the content of VCHs in the underground water was 1000. Mu.g/L, and the amount of the sustained-release agent added was 1.0g in 1L of the underground water. And a comparative example is added, namely no medicament is injected. The test was carried out according to the method of example 1, and the degradation rates of dichloroethylene, dichloromethane, trichloromethane, dichloroethane and chlorobenzene in the groundwater after 3d of operation were 23.37%, 18.26%, 16.63%, 12.77% and 26.56%, respectively. After the operation is continued for 7d, the degradation rates of the dichloroethylene, the dichloromethane, the trichloromethane, the dichloroethane and the chlorobenzene in the underground water are respectively 55.56 percent, 60.35 percent, 87.02 percent, 82.76 percent and 85.71 percent. The amount of indigenous microbes in the groundwater increased from 10.10 ng/. Mu.L to 13.70 ng/. Mu.L, and the abundance of Proteobacteria, actinobacilla, bacteroides, and Gemmatimadides increased by 6.70% -8.28%.
Example 3
Combining the hydrogeological conditions of a VCHs polluted site, based on an in-situ injection restoration engineering mode, manufacturing an in-situ injection simulation system by adopting an organic glass tank, wherein main components of the system comprise a tank for containing a VCHs (containing dichloroethylene, dichloromethane, dichloroethane, trichloromethane and chlorobenzene) polluted water solution, a peristaltic pump, organic glass tanks (the length, the width and the height of each tank are 50cm, 30cm and 30 cm) and a waste liquid barrel, water distribution plates (the aperture is 2.5mm and the hole distance is 0.5 cm) with the length of 5cm are arranged at two ends in each organic glass tank, water is uniformly distributed at water inlets, the water level of the water inlets is controlled by the peristaltic pumps, water at water outlets at the tail ends is mixed water, and the hydraulic gradient is about 5 per thousand, the method comprises the following steps that internal lithologic media comprise 5cm thick gravel, 5cm thick coarse sand, 6cm thick medium sand, 6cm thick fine sand and 5cm thick silty clay from bottom to top in sequence, wherein a silty clay layer serves as a top plate of an aquifer, lateral sampling holes are arranged in the middle of different lithologic layers of the aquifer, the horizontal distance between the sampling holes is 5cm, ports 3 of parallel layered slow-release agent injection holes are formed in the positions 15cm away from the horizontal direction of a water inlet and used for directionally adding nutrient matrix slow-release agents into the lithologic media at different layers, the top layer covers 4cm of the silty clay layer, after filling of an organic tank is finished, indigenous microbial liquid is added into ultrapure water and then the lithologic media are saturated, so that microorganisms enter the lithologic media, and the VCHs effect of indigenous functional microorganisms in the simulated lithologic media is contrastively degraded.
The concentration of each component of underground water simulation solution VCHs (containing dichloroethylene, dichloromethane, dichloroethane, trichloromethane and chlorobenzene) is 1000 mug/L, the concentration of the VCHs in an aquifer is stable and tends to the concentration of raw water when the VCHs polluted solution continuously enters 60 hours, the nutrient substrate slow-release agent (1 g/L) in the embodiment 1 is injected into each lithologic layer through a floral tube, and the operation lasts for 10 days. Sampling is carried out on lateral sampling holes of each layer corresponding to the injection well and the downstream thereof at time points of 12h, 24h, 2d, 3d, 5d, 7d and 10d respectively, and monitoring indexes comprise VCHs components and slow release indicator ions (Mg) 2+ 、K + ) And indigenous microbial flora gene detection (DNA content and flora composition).
Test results show that the degradation effect of VCHs is obviously increased along with the change of time, the degradation effect of indigenous microorganisms reaches dynamic balance after the 2d starts to continuously act for 10d, the degradation rates (%) of DCE, DCM, TCM, EDC and MCB are 93.52, 88.24, 88.00, 81.02 and 76.78 respectively, and indicator ions (Mg) are slowly released (Mg is used for indicating the ion release, and the degradation rate (%) of the DCE, DCM, TCM, EDC and MCB is 93.52, 88.24, 88.00, 81.02 and 76.78 respectively) 2+ 、K + ) The content is about 10mg/L; after the sustained-release agent is added, the DNA content of the indigenous functional microbial flora is respectively increased to 12.4, 24.8, 20 and 18.5mg/L corresponding to 12.1, 19.7, 16 and 11.2mg/L of fine sand, medium sand, coarse sand and gravel layer before being added, the microbial flora is mainly Proteobacteria, actinomycetes and Bacteroidetes before the sustained-release agent is added, the abundance value is 91.85-99.01%, the abundance of actinomycetes, bacteroidetes and gemmationeads in fine sand layer after the sustained-release agent is added is increased by 37.60%, and the abundance of firmutes population is reduced by 3.93%; for other sand layers, the abundance of the Firmicutes population is obviously increased after the slow release medicament is added, and the abundance is increased by 22.66% -25.88%, the abundance of Candidatus Saccharomyces cerevisiae population is reduced, wherein Proteobacteria and Firmicutes are VCHs degrading bacteria, and Bacteroides can positively promote the degradation of VCHs through a metabolic process. According to the principle that the slow release agent of the invention slowly releases and stimulates the degradation of indigenous microorganisms in the VCHs polluted underground water, the slow release stimulation effect of the slow release agent in the VCHs polluted underground water can be maintained for 30 days by calculating the equation of Highchi and Korsmeyer-Peppas.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (4)
1. A nutrient substrate slow-release medicament for stimulating bioremediation of VCHs polluted groundwater is characterized by comprising the following steps:
s1, preparing a nutrient medium
The nutrient medium comprises the following components in parts by weight:
NH 4 cl0.5-1.5 parts, KNO 3 40 to 60 portions of Na 2 HPO 4 80 to 120 portions of CaCl 2 5-15 parts of MgSO (MgSO) 4 400-550 parts of MnSO 4 0.05 to 0.2 portion of ZnCl 2 0.5-2 parts of, CH 3 0.5-2 parts of COOH;
s2, coating treatment
(1) Mixing the wall material and the nutrient medium, putting the mixture into water, and uniformly stirring the mixture at the temperature of 45-55 ℃ to obtain a mixed solution; the mass ratio of the wall material to the nutrient medium to the water is 3-5:1:100;
(2) Putting ethanol into a container and placing the container in an ultrasonic instrument for ultrasonic treatment; cooling the mixed solution to room temperature, then dropwise adding the mixed solution into the container filled with ethanol, and continuing ultrasonic treatment to obtain a suspension;
(3) Centrifuging the suspension to obtain a solid-phase material;
(4) And finally, washing and drying the solid phase material to obtain the nutrient matrix sustained-release medicament.
2. The sustained-release nutrient matrix preparation for stimulating bioremediation of VCHs contaminated groundwater as claimed in claim 1, wherein the wall material is starch.
3. The sustained-release nutrient substrate reagent for stimulating bioremediation of underground water polluted by VCHs according to claim 1, wherein the dropping speed in the step (2) is 5-15mL/min.
4. The sustained-release medicament for stimulating the bioremediation of the nutrient substrate of the groundwater polluted by VCHs as claimed in claim 1, wherein the centrifugation speed in the step (3) is 5000-8000rpm, and the centrifugation time is 10-15 min.
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| CN202211431052.5A CN115893679A (en) | 2022-11-15 | 2022-11-15 | Nutrient matrix sustained-release medicament for stimulating bioremediation of VCHs polluted underground water |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030113903A1 (en) * | 2001-09-10 | 2003-06-19 | Fuji Photo Film Co., Ltd. | Method of remedying contaminated soil by microorganism |
| CN105668689A (en) * | 2016-04-11 | 2016-06-15 | 华东理工大学 | Multifunctional slow-release restoration agent applied to underground water in-situ restoration |
| US20170333962A1 (en) * | 2016-05-19 | 2017-11-23 | Remediation Products, Inc. | Bioremediation composition with a time release material for removing hydrocarbons from contaminated environments |
-
2022
- 2022-11-15 CN CN202211431052.5A patent/CN115893679A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030113903A1 (en) * | 2001-09-10 | 2003-06-19 | Fuji Photo Film Co., Ltd. | Method of remedying contaminated soil by microorganism |
| CN105668689A (en) * | 2016-04-11 | 2016-06-15 | 华东理工大学 | Multifunctional slow-release restoration agent applied to underground water in-situ restoration |
| US20170333962A1 (en) * | 2016-05-19 | 2017-11-23 | Remediation Products, Inc. | Bioremediation composition with a time release material for removing hydrocarbons from contaminated environments |
Non-Patent Citations (1)
| Title |
|---|
| 尹斯琦: "基于生物刺激修复VCHs污染地下水的营养基质缓释药剂研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》, 15 January 2022 (2022-01-15), pages 027 - 628 * |
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