CN115888652A - Preparation method of hypha-based biochar - Google Patents
Preparation method of hypha-based biochar Download PDFInfo
- Publication number
- CN115888652A CN115888652A CN202211505582.XA CN202211505582A CN115888652A CN 115888652 A CN115888652 A CN 115888652A CN 202211505582 A CN202211505582 A CN 202211505582A CN 115888652 A CN115888652 A CN 115888652A
- Authority
- CN
- China
- Prior art keywords
- mycelium
- biochar
- based biochar
- following
- steps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 11
- 150000002500 ions Chemical class 0.000 claims abstract description 10
- 238000001179 sorption measurement Methods 0.000 claims abstract description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 244000252132 Pleurotus eryngii Species 0.000 claims description 10
- 235000001681 Pleurotus eryngii Nutrition 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 238000003763 carbonization Methods 0.000 claims description 7
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 238000004438 BET method Methods 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000010000 carbonizing Methods 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 3
- 239000008158 vegetable oil Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims 1
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of hypha-based biochar. The hypha-based biochar has high adsorption performance, and can effectively adsorb and remove heavy metal ions in the environment.
Description
Technical Field
The invention belongs to the technical field, and particularly relates to a preparation method of hypha-based biochar.
Background
The biochar is a solid byproduct generated by high-temperature cracking of biomass materials in an anoxic or low-oxygen environment, and has the characteristics of high aromaticity, high carbon content, strong capability of resisting biological and non-biological degradation and the like.
The biochar has a wide range of applications, and can be applied to soil to increase soil fertility and reduce greenhouse gas emission. In addition, the biochar has larger specific surface area and higher porosity, can adsorb and degrade toxic and harmful substances in the environment, including various heavy metal ions, organic pollutants and the like, and plays an important role in the aspects of environmental pollution treatment, ecological restoration and the like.
The fungal hyphae are rich in chitin, lignin compounds, aromatic compounds and the like, and have high growth speed and rich sources. The mycelium-based biochar prepared by taking the fungal mycelium as a material has the characteristics of wide source, low cost, high adsorption activity and the like, and can be widely applied to removal of various pollutants in the environment.
Pleurotus eryngii (Pleurotus eryngii) is a common edible fungus, and its mycelium is easy to culture and can be produced in large scale. The method takes pleurotus eryngii mycelium as a raw material, activates the mycelium by using sodium hydroxide, and then prepares the mycelium-based biochar under the high-temperature condition. The hypha-based biochar has high adsorption performance, and can effectively adsorb and remove heavy metal ions in the environment.
Disclosure of Invention
The invention aims to provide a preparation method of hypha-based biochar, which has high adsorption performance and can effectively adsorb and remove heavy metal ions in the environment.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of hypha-based biochar is characterized by comprising the following steps: the method comprises the following steps:
preparing a PDA slant culture medium, inoculating a strain block with the diameter of 0.5 cm into the PDA slant culture medium, and culturing at 25 ℃ for 8 days until hyphae basically grow over the whole slant;
inoculating a slant strain into 100 mL of seed solution, and performing shake culture at 25 ℃ for 7 days to obtain the seed solution;
inoculating the seed liquid into a pneumatic fermentation tank by using a fermentation culture medium, wherein the inoculation amount is 2%, ventilating and stirring for culture, and culturing for 7 days at 20 ℃;
after the fermentation culture in the step (4) is finished, taking out the culture solution from the fermentation tank, and centrifuging for 10 minutes at 3000 rotating speed; then, washing with distilled water for three times, and centrifuging at 3000 rpm for 10 minutes each time to obtain pure Pleurotus eryngii mycelium;
step (5) performing freeze drying treatment on the mycelium by using a vacuum freeze dryer, and storing at 4 ℃ for later use;
weighing the mycelium in the step (5), grinding and crushing the mycelium, sieving the ground mycelium with a 100-mesh sieve, then soaking the mycelium in 200 mL of 2% sodium hydroxide solution for 2 hours, washing the mycelium with ultrapure water to be neutral, and drying the mycelium at 80 ℃ for 12 hours;
step (7), placing the modified mycelium into a crucible and placing the crucible into a muffle furnace; respectively carbonizing for 2 h at 400-600 ℃;
step (8) grinding and crushing the biochar obtained in the step (7), sieving the biochar with a 100-mesh sieve, and filling the biochar into a dryer for storage for later use;
and (9) analyzing the specific surface area of the biochar by a BET method by using a full-automatic specific surface area analyzer, and determining the optimal carbonization temperature and high-quality biochar.
Further, the PDA culture medium raw materials in the step (1) comprise: 200 g of potato, 20 g of glucose, 20 g of agar and 1000 mL of distilled water.
Further, the seed liquid raw materials in the step (2) comprise: 30 g of glucose, 2 g of peptone, 5 g of yeast powder, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 0.01 g of vitamin B and 1000 mL of distilled water.
Further, the fermentation medium raw materials in the step (3) comprise: 15 g of glucose, 3 g of soybean meal, 3 g of corn flour, 1 g of bran, 0.5 g of peptone, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 0.2 mL of vegetable oil, 1000 mL of distilled water and pH of 6.0-6.5.
Furthermore, 600 ℃ in the step (7) is a more suitable carbonization temperature, and the specific surface area is 102.37 m2/g.
The application of the hypha-based biochar is that the hypha-based biochar has good adsorption performance and can be used for removing heavy metal ions in the environment.
The technical scheme can obtain the following beneficial effects:
the method takes pleurotus eryngii mycelium as a raw material, activates the mycelium by using sodium hydroxide, and then prepares the mycelium-based biochar under the high-temperature condition. The hypha-based biochar has high adsorption performance, and can effectively adsorb and remove heavy metal ions in the environment.
Detailed Description
The invention is further illustrated by the following examples:
1. and (3) culturing mycelium:
1.1 Culture medium
(1) PDA culture medium: 200 g of potatoes, 20 g of glucose, 20 g of agar and 1000 mL of distilled water.
(2) Seed culture medium: 30 g of glucose, 2 g of peptone, 5 g of yeast powder, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate and vitamin B 1 0.01 g, 1000 mL of distilled water.
(3) Fermentation medium: 15 g of glucose, 3 g of soybean meal, 3 g of corn flour, 1 g of bran, 0.5 g of peptone, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 0.2 mL of vegetable oil, 1000 mL of distilled water and pH of 6.0-6.5.
1.2 Cultivation process
(1) Preparing a PDA slant culture medium, inoculating a strain block with a diameter of 0.5 cm into the PDA slant culture medium, and culturing at 25 deg.C for 8 days until the mycelia substantially grow over the whole slant.
(2) A slant strain was inoculated into 100 mL of the seed solution, and the mixture was shake-cultured at 25 ℃ for 7 days, which was the seed solution.
(3) Inoculating the seed liquid into a pneumatic fermentation tank by using a fermentation culture medium, wherein the inoculation amount is 2%, and the seed liquid is subjected to aeration stirring culture at 20 ℃ for 7 days.
(4) After the completion of the fermentation culture, the culture broth was taken out from the fermentor and centrifuged at 3000 rpm for 10 minutes. Then, the pleurotus eryngii is washed by distilled water for three times, and each time, the pleurotus eryngii is centrifuged at 3000 rpm for 10 minutes to obtain pure pleurotus eryngii mycelium.
(5) The mycelium is freeze-dried by a vacuum freeze-drying instrument and stored at 4 ℃ for later use.
2. Preparation of hypha-based biochar
(1) Weighing 15 g of mycelium accurately, grinding and crushing the mycelium, sieving the mycelium by a 100-mesh sieve, then soaking the mycelium in 200 mL of 2% sodium hydroxide solution for 2 h, washing the mycelium to be neutral by ultrapure water, and drying the mycelium at 80 ℃ for 12 h.
(2) Placing the modified mycelium into a crucible and placing the crucible into a muffle furnace; carbonizing for 2 h at 400 deg.C, 500 deg.C and 600 deg.C respectively.
(3) Grinding and crushing the obtained biochar, sieving the biochar with a 100-mesh sieve, and putting the biochar into a dryer for storage for later use.
(4) The specific surface area of the biochar was analyzed by the BET method using a full-automatic specific surface area analyzer, and the results are shown in table 1. In the preparation process of the biochar, the influence of the carbonization temperature on the internal structure of the biochar is large, 600 ℃ is found to be a relatively proper carbonization temperature, and the specific surface area is 102.37 m 2 /g。
TABLE 1 specific surface area of mycelial-based biochar at different temperatures
| Preparation temperature (. Degree.C.) | Specific surface area (m) 2 /g） |
| 400 | 25.48 |
| 500 | 56.24 |
| 600 | 102.37 |
3. Application of hypha-based biochar
(1) Respectively preparing 20 mg/L Cu 2+ 、Pb 2+ 、Cd 2+ The pH of the solution was adjusted to 6.0, and 50 mL of the solution was placed in a 250 mL Erlenmeyer flask.
(2) Adding 0.05 g of hypha-based biochar, then placing the triangular flask in a shaking table, and shaking for 24 hours at 180 r/min.
(3) After adsorption, taking the supernatant, filtering the supernatant through a 0.22 micron filter membrane, and measuring the residual Cu in the filtrate by using an atomic absorption spectrophotometer 2+ 、Pb 2+ 、Cd 2+ And (4) concentration.
(4) The mycelium-based biochar has strong heavy metal ion adsorption capacity, and when the input amount is 1 g/L, the mycelium-based biochar can adsorb Cu 2+ 、Pb 2+ 、Cd 2+ The removal rates of (a) were 91.38%, 92.67% and 90.28%, respectively (table 2).
TABLE 2 removal rate of heavy metal ions by hypha-based biochar
| Class of heavy metals | Cu 2+ | Pb 2+ | Cd 2+ |
| Removal Rate (%) | 91.38% | 92.67% | 90.28% |
The method takes pleurotus eryngii mycelium as a raw material, activates the mycelium by using sodium hydroxide, and then prepares mycelium-based biochar at a high temperature, wherein the temperature of 600 ℃ is a relatively proper carbonization temperature.
The hypha-based biochar has good adsorption performance, and can effectively remove heavy metal ions in the environment.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A preparation method of hypha-based biochar is characterized by comprising the following steps: the method comprises the following steps:
preparing a PDA slant culture medium, inoculating a strain block with the diameter of 0.5 cm into the PDA slant culture medium, and culturing at 25 ℃ for 8 days until hyphae basically grow over the whole slant;
inoculating a slant strain into 100 mL of seed solution, and performing shake culture at 25 ℃ for 7 days to obtain the seed solution;
inoculating the seed liquid into a pneumatic fermentation tank by using a fermentation culture medium, wherein the inoculation amount is 2%, ventilating and stirring for culture, and culturing for 7 days at 20 ℃;
after the fermentation culture in the step (4) is finished, taking out the culture solution from the fermentation tank, and centrifuging for 10 minutes at 3000 rotating speed; then, washing with distilled water for three times, and centrifuging at 3000 rpm for 10 minutes each time to obtain pure Pleurotus eryngii mycelium;
step (5) performing freeze drying treatment on the mycelium by using a vacuum freeze dryer, and storing at 4 ℃ for later use;
weighing the mycelium in the step (5), grinding and crushing the mycelium, sieving the ground mycelium with a 100-mesh sieve, then soaking the mycelium in 200 mL of 2% sodium hydroxide solution for 2 hours, washing the mycelium with ultrapure water to be neutral, and drying the mycelium at 80 ℃ for 12 hours;
step (7), placing the modified mycelium into a crucible and placing the crucible into a muffle furnace; respectively carbonizing for 2 hours at the preparation temperature of 400-600 ℃;
step (8) grinding and crushing the biochar obtained in the step (7), sieving the biochar with a 100-mesh sieve, and putting the biochar into a dryer for storage for later use;
and (9) analyzing the specific surface area of the biochar by a BET method by using a full-automatic specific surface area analyzer, and determining the optimal carbonization temperature and high-quality biochar.
2. The method for preparing a mycelial-based biochar according to claim 1, wherein the method comprises the following steps: the PDA culture medium in the step (1) comprises the following raw materials: 200 g of potato, 20 g of glucose, 20 g of agar and 1000 mL of distilled water.
3. The method for preparing a mycelial-based biochar according to claim 1, wherein the method comprises the following steps: the seed liquid in the step (2) comprises the following raw materials: 30 g of glucose, 2 g of peptone, 5 g of yeast powder, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate and vitamin B 1 0.01 g, 1000 mL of distilled water.
4. The method for preparing a mycelial-based biochar according to claim 1, wherein the method comprises the following steps: the fermentation medium in the step (3) comprises the following raw materials: 15 g of glucose, 3 g of bean pulp, 3 g of corn flour, 1 g of bran, 0.5 g of peptone, 0.5 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 0.2 mL of vegetable oil, 1000 mL of distilled water and pH of 6.0-6.5.
5. The method for preparing a mycelial-based biochar according to claim 1, wherein the method comprises the following steps: the temperature of 600 ℃ in the step (7) is a more proper carbonization temperature, and the specific surface area is 102.37 m 2 /g。
6. The application of the mycelium-based biochar is characterized in that: the hypha-based biochar has good adsorption performance, and can be used for removing heavy metal ions in the environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211505582.XA CN115888652A (en) | 2022-11-29 | 2022-11-29 | Preparation method of hypha-based biochar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211505582.XA CN115888652A (en) | 2022-11-29 | 2022-11-29 | Preparation method of hypha-based biochar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115888652A true CN115888652A (en) | 2023-04-04 |
Family
ID=85731792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211505582.XA Pending CN115888652A (en) | 2022-11-29 | 2022-11-29 | Preparation method of hypha-based biochar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115888652A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110508243A (en) * | 2019-08-14 | 2019-11-29 | 贵州大学 | A kind of preparation method and application of biomass-based porous charcoal load iron wadding body adsorbent material |
| CN113198418A (en) * | 2021-04-19 | 2021-08-03 | 贵州民族大学 | Method for preparing efficient phosphorus removal activated carbon by using edible fungus residues |
| CN113828277A (en) * | 2021-10-28 | 2021-12-24 | 重庆科技学院 | Modified biochar and preparation method and application thereof |
| CN114870800A (en) * | 2022-05-05 | 2022-08-09 | 东南大学 | Modified biochar/attapulgite composite material, and preparation method and application thereof |
| WO2022199308A1 (en) * | 2021-03-26 | 2022-09-29 | 中国环境科学研究院 | Porous biochar for improving severe pollution and preparation method therefor |
| CN115121235A (en) * | 2021-03-29 | 2022-09-30 | 湖南大学 | Method for recycling edible fungus vegetable biochar capable of adsorbing heavy metals |
-
2022
- 2022-11-29 CN CN202211505582.XA patent/CN115888652A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110508243A (en) * | 2019-08-14 | 2019-11-29 | 贵州大学 | A kind of preparation method and application of biomass-based porous charcoal load iron wadding body adsorbent material |
| WO2022199308A1 (en) * | 2021-03-26 | 2022-09-29 | 中国环境科学研究院 | Porous biochar for improving severe pollution and preparation method therefor |
| CN115121235A (en) * | 2021-03-29 | 2022-09-30 | 湖南大学 | Method for recycling edible fungus vegetable biochar capable of adsorbing heavy metals |
| CN113198418A (en) * | 2021-04-19 | 2021-08-03 | 贵州民族大学 | Method for preparing efficient phosphorus removal activated carbon by using edible fungus residues |
| CN113828277A (en) * | 2021-10-28 | 2021-12-24 | 重庆科技学院 | Modified biochar and preparation method and application thereof |
| CN114870800A (en) * | 2022-05-05 | 2022-08-09 | 东南大学 | Modified biochar/attapulgite composite material, and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| SILIN YANG等: "Biosorption of Cu(II) from Aqueous Solutions by a Macrofungus (Ganoderma lobatum) Biomass and its Biochar", 《NAT. ENV. & POLL. TECH.》, vol. 20, no. 2, 31 December 2021 (2021-12-31), pages 579 * |
| 吴祖峰等: "杏鲍菇液体发酵的初步研究", 《中国优秀硕士学位论文全文数据库农业科技辑》, no. 6, 15 December 2007 (2007-12-15), pages 2 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107088399B (en) | Preparation method and application of mushroom bran biochar adsorbent | |
| Fan et al. | Use of various coffee industry residues for the cultivation of Pleurotus ostreatus in solid state fermentation | |
| CN105837005B (en) | Municipal biological dewatered organic conditioner of excess sludge and preparation method thereof | |
| CN112758924B (en) | Graphene material composite biomass activated carbon and preparation method thereof | |
| CN110157624B (en) | Paecilomyces lilacinus large-scale production method based on automatic starter propagation machine | |
| CN113952926A (en) | Method for synchronously removing arsenic and organic pollutants by using loaded ferrihydrite nanoparticle biochar prepared by combining biological/chemical means | |
| CN114471463B (en) | Biochar for adsorbing heavy metal cadmium and preparation method and application thereof | |
| CN114456942B (en) | Chain belt algae for treating high-concentration molasses alcohol wastewater and application thereof | |
| CN115888652A (en) | Preparation method of hypha-based biochar | |
| CN113597974B (en) | Edible fungus renewable culture medium, preparation method and application | |
| CN115466685B (en) | Fusarium vine bin and gibberellin A produced by fermentation of Fusarium vine bin 4+7 The method and the application of (2) | |
| CN111548967B (en) | Pseudomonas putida X14 and application method thereof | |
| CN110042063B (en) | Gliocladium roseum (Clinostacchys rosea) strain YZC3 and application thereof | |
| CN109046271B (en) | Preparation method of water treatment adsorbent based on composite biomass-based material | |
| CN113181876A (en) | Method for enhancing adsorption performance of wheat straw carbon by utilizing filamentous fungus fermentation technology and application | |
| CN109824392B (en) | Organic fertilizer and preparation method thereof | |
| CN113996270A (en) | Preparation and application of modified medlar branch adsorbent | |
| CN1948453A (en) | Method of screening laccase endogenic bateria from Chinese sumac and preparing laccase through solid state fermentation | |
| CN113663640A (en) | Hierarchical porous carbon material and preparation method and application thereof | |
| CN112845566A (en) | Method for repairing phthalate polluted soil by using lipase | |
| CN117801965B (en) | Method for preparing biological sponge for adsorbing sewage by using cordyceps militaris and application of biological sponge | |
| CN116218929B (en) | Fermentation crude extract, preparation method and application | |
| CN112746020A (en) | Penicillium for producing biosurfactant and application thereof | |
| CN112980698B (en) | Microbial fermentation carbon source and preparation method thereof | |
| CN1285720C (en) | Cordyceps vegetative stage solid fermentation technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |