CN115873707B - Cell harvesting system - Google Patents
Cell harvesting system Download PDFInfo
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- CN115873707B CN115873707B CN202211708015.4A CN202211708015A CN115873707B CN 115873707 B CN115873707 B CN 115873707B CN 202211708015 A CN202211708015 A CN 202211708015A CN 115873707 B CN115873707 B CN 115873707B
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- 238000003306 harvesting Methods 0.000 title claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 131
- 238000002347 injection Methods 0.000 claims abstract description 73
- 239000007924 injection Substances 0.000 claims abstract description 73
- 230000007246 mechanism Effects 0.000 claims abstract description 35
- 238000001816 cooling Methods 0.000 claims abstract description 11
- 239000003507 refrigerant Substances 0.000 claims abstract description 8
- 239000012774 insulation material Substances 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 230000033001 locomotion Effects 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 7
- 239000004020 conductor Substances 0.000 claims description 7
- 239000000523 sample Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000011810 insulating material Substances 0.000 claims description 5
- 239000000815 hypotonic solution Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 abstract description 16
- 210000004027 cell Anatomy 0.000 description 35
- 239000010410 layer Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 230000037452 priming Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000003028 elevating effect Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
Abstract
The invention relates to a cell harvesting system, which comprises a bedplate (1), wherein a constant-temperature centrifugal bin (3) and an oscillating mechanism (5) are arranged below the bedplate (1), a liquid suction and injection mechanism (4) and a liquid path module (6) connected with the liquid suction and injection mechanism are arranged above the bedplate (1), a centrifugal mechanism (2) is arranged in the constant-temperature centrifugal bin (3), the constant-temperature centrifugal bin 3 comprises a double-layer shell (31), a heat insulation material (32), a heat conduction material (33), a condenser coil (34), a direct cooling type constant-temperature device (35) and a plurality of stop valves (36), the section of the condenser coil (34) is of a hollow structure and is internally communicated with a refrigerant (341), the condenser coil (34) is integrally of a spiral structure and surrounds the inside of the double-layer shell (31), a surrounding gap of the condenser coil (34) is filled with the heat conduction material (33), and the periphery is provided with the heat insulation material (32). The device can improve heating efficiency and energy utilization rate, and can make the liquid suction and injection of the harvesting system more efficient.
Description
Technical Field
The invention belongs to the technical field of medical auxiliary equipment, and relates to a cell harvesting system.
Background
In a cell harvesting system, a constant temperature environment plays a critical role in the quality of the harvested chromosome. In order to keep a constant temperature environment in the cell harvesting process, the conventional cell harvesting system is generally provided with a heating device in a centrifugal bin, so that the centrifugal bin is large in size, and the control implementation difficulty is high due to dynamic heating. In addition, heating device is generally heater strip coil, go up heating ring or a plurality of hot plates, leads to the calorific capacity in the twinkling of an eye big, and the condition that the temperature is undulant by a wide margin appears after the heating extremely easily, after the constant temperature that heating temperature exceeded setting up, can only wait centrifugal storehouse self heat dissipation, spends the time for a long time, and the temperature control effect is not ideal, and the temperature homogeneity is poor.
In addition, the heating mode of the traditional cell harvesting system is that the heating is carried out through the resistor, the theoretical thermal efficiency value is 100%, and the centrifugal bin lacks heat preservation measures, so that heat is dissipated from the centrifugal bin while the resistor is heated. In the whole harvesting process, the power consumption of the light centrifugal bin heating function can be about 1/3 of the total power consumption, and great energy waste is caused.
In addition, hypotonic solution and fixed solution are needed to be added in the cell harvesting process, and supernatant after centrifugation is needed to be sucked out, so that a liquid injection mechanism and a liquid suction mechanism are generally adopted to be independently designed in a harvesting system on the market at present, the structure is complex, the cost is increased, and meanwhile, the miniaturization of equipment is not facilitated.
In view of the above-mentioned technical drawbacks of the prior art, there is an urgent need to develop a novel cell harvesting system.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a high-efficiency cell harvesting system which can improve heating efficiency and energy utilization rate and enable liquid suction and injection of the harvesting system to be more efficient.
In order to achieve the above object, the present invention provides the following technical solutions:
the utility model provides a cell harvesting system, its includes the platen, be equipped with constant temperature centrifugation storehouse and oscillating mechanism below the platen, be equipped with above the platen imbibition annotate liquid mechanism and with liquid way module that liquid mechanism links to each other is annotated to the imbibition, be equipped with centrifugal mechanism in the constant temperature centrifugation storehouse, its characterized in that, constant temperature centrifugation storehouse includes bilayer shell, heat-insulating material, heat-conducting material, condenser coil pipe, direct cooling type constant temperature equipment and a plurality of stop valve, the cross-section of condenser coil pipe is hollow structure and its inside has led to the refrigerant, the condenser coil pipe is whole to be spiral structure and encircle bilayer shell's inside, the clearance intussuseption is filled with around the condenser coil pipe heat-conducting material, periphery then set up heat-insulating material.
Preferably, the direct cooling type constant temperature device comprises a thermal expansion valve, an evaporator and a compressor, wherein one end of the thermal expansion valve is connected with one end of the condenser coil through one of the stop valves, the other end of the thermal expansion valve is connected with one end of the evaporator, the other end of the evaporator is connected with one end of the compressor, and the other end of the compressor is connected with the other end of the condenser coil through the other stop valve.
Preferably, a plurality of temperature probes are further arranged in the constant temperature centrifugal bin, and the direct cooling type constant temperature device further comprises an electric temperature control valve, one end of the electric temperature control valve is connected between the thermal expansion valve and the evaporator, and the other end of the electric temperature control valve is connected between the compressor and the other stop valve.
Preferably, the liquid suction and injection mechanism comprises a rotary lifting module, a rotary rod, a liquid injection station and a liquid suction station, wherein the upper end of the rotary lifting module is fixedly connected with the rotary rod, the liquid injection station and the liquid suction station are respectively fixed at two ends of the rotary rod, and the rotary lifting module can drive the rotary rod to realize up-and-down movement and rotary movement.
Preferably, the liquid injection station comprises a liquid injection rotating motor, a liquid injection rotating disc, a low-permeability liquid injection needle and a fixed liquid injection needle, wherein the liquid injection rotating motor is used for driving the liquid injection rotating disc to rotate, and the low-permeability liquid injection needle and the fixed liquid injection needle are installed on the circumference of the liquid injection rotating disc at intervals.
Preferably, the pipetting station comprises a pipetting rotor and pipetting needles mounted on the circumference of the pipetting rotor.
Preferably, the centrifugal mechanism comprises a rotary table, an adapter with a swinging rod, a centrifugal motor and a transmission shaft, wherein a plurality of adapters with swinging rods are arranged on the rotary table, and the lower side of the rotary table is connected with the centrifugal motor through the transmission shaft.
Preferably, a plurality of centrifuge tube holes are formed in each adapter with the swing rod, the hypotonic liquid injection needle and the fixed liquid injection needle are in one-to-one correspondence with the centrifuge tube holes in the adapter with the swing rod, and the liquid suction needles are also in one-to-one correspondence with the centrifuge tube holes in the adapter with the swing rod.
Preferably, the liquid path module comprises a hypotonic liquid reagent bottle, a metering pump, a fixed liquid reagent bottle, a waste liquid collecting bottle, a distilled water bottle, a diaphragm pump and a cleaning bin, wherein the hypotonic liquid reagent bottle is connected with the hypotonic liquid injection needle through one of the metering pumps, the fixed liquid reagent bottle is connected with the fixed liquid injection needle through the other one of the metering pumps, the waste liquid collecting bottle is connected with the liquid suction needle through one of the diaphragm pumps, and the distilled water bottle is connected with the cleaning bin through the other one of the diaphragm pumps.
Preferably, the oscillating mechanism comprises a fixed plate, a screw nut, a screw rod, a coupler, an oscillating lifting motor, an oscillating motor and an eccentric tray, wherein the lower end of the screw rod is connected with an output shaft of the oscillating lifting motor through the coupler, the screw nut and the oscillating motor are arranged on the fixed plate, the screw rod is sleeved with the screw nut, and the eccentric tray is connected with the output shaft of the oscillating motor.
Compared with the prior art, the cell harvesting system of the invention has one or more of the following beneficial technical effects:
1. the constant temperature centrifugal bin adopts a heat pump technology, and a series of new technologies such as a spiral condenser coil pipe and the like are installed in an interlayer of the centrifugal bin, so that the heat energy of a low-level heat source is transferred to a high-level heat source by applying the reverse Carnot cycle principle, the heat energy carrying capacity is strong, and the equivalent heating effect can be achieved only by about 1/4 of the energy consumption of the traditional heating mode.
2. The constant-temperature centrifugal bin is also provided with a double-layer shell in design, and the side in contact with the outer layer is filled with a heat insulation material, so that the conduction of heat to the outside of the centrifugal bin is weakened; the side contacted with the inner layer is filled with heat conducting materials in gaps around the condenser coil, so that heat conduction with the inner layer shell is enhanced, and further heat energy is better conducted inwards; meanwhile, the design of the spiral condenser coil greatly increases the contact area between the spiral condenser coil and the inside of the centrifugal bin, so that the temperature uniformity in the bin is better controlled; therefore, the problem of nonuniform temperature and unstable temperature control in the centrifugal bin is effectively solved, the cell harvesting process can be kept in a constant temperature state all the time, and the cell harvesting effect is better.
3. The liquid suction and injection process is highly integrated, and the problems of huge harvesting system, complex control, high energy consumption and high production, use and maintenance cost in the past are solved.
4. The method makes the constant temperature control level in the cell harvesting process a brand-new step, and can further improve the cell harvesting effect, chromosome quantity and quality; the integrated design of liquid suction and injection simplifies the structure, reduces the cost and enables the harvesting system to be more miniaturized.
Drawings
FIG. 1 is a schematic diagram of the cell harvesting system of the present invention.
FIG. 2 is a front view of the cell harvesting system of the present invention.
FIG. 3 is a partial schematic view of the cell harvesting system of the present invention.
FIG. 4 is a schematic partial cross-sectional view of a thermostatic centrifugal cartridge of the cell harvesting system of the present invention.
FIG. 5 is a schematic diagram of the structure of a condenser coil of the cell harvesting system of the present invention.
FIG. 6 is a schematic diagram of the operation of the thermostatic centrifugal cartridge of the cell harvesting system of the present invention.
FIG. 7 is a schematic diagram of the structure of the oscillating mechanism of the cell harvesting system of the present invention.
In the figure, 1 is a platen, 11 is a centrifugal bin sealing plate, 12 is a centrifugal bin door, 2 is a centrifugal mechanism, 21 is a turntable, 22 is an adapter with a swinging rod, 23 is a centrifugal motor, 24 is a transmission shaft, 3 is a constant-temperature centrifugal bin, 31 is a double-layer shell, 32 is a heat insulation material, 33 is a heat conduction material, 34 is a condenser coil, 341 is a refrigerant, 35 is a direct-cooling type constant-temperature device, 351 is a thermal expansion valve, 352 is an evaporator, 353 is a compressor, 354 is an electric temperature control valve, 36 is a stop valve, 37 is a temperature probe, 4 is a liquid suction and injection mechanism, 41 is a rotary lifting module, 42 is a rotary rod, 43 is a liquid injection station, 431 is a liquid injection rotary motor, 432 is a liquid injection turntable, 433 is a low-permeation liquid injection needle, 434 is a fixed liquid injection needle, 44 is a liquid suction station, 441 is a liquid suction turntable, 442 is a needle, 5 is an oscillating mechanism, 51 is a fixed plate, 52 is a lead screw nut, 53 is a lead screw, 54 is a coupling, 55 is an oscillating lifting motor, 56 is an oscillating motor, 57 is an eccentric tray, 6 is a liquid channel 61 is a liquid pump, 62 is a two-way valve, 64 is a liquid suction bottle, 69 is a liquid suction bottle is a liquid collection bottle, and 65 is a liquid bottle is a liquid collection bottle.
Detailed Description
The invention is further illustrated by the following examples in conjunction with the accompanying drawings, which are not to be construed as limiting the scope of the invention.
The invention relates to a high-efficiency cell harvesting system which can be used for the middle-stage chromosome harvesting process of peripheral blood, bone marrow, amniotic fluid, umbilical cord blood, chorionic cells and the like, and realizes the accurate constant-temperature environmental control of the whole harvesting process, and has the advantages of quick temperature heating, stable control, quick response speed and simple maintenance.
As shown in fig. 1-3, the cell harvesting system of the present invention comprises a platen 1. The platen 1 serves to support and carry other components.
A constant temperature centrifugal bin 3 and an oscillating mechanism 5 are arranged below the bedplate 1. The upper surface of the bedplate 1 is provided with a liquid suction and injection mechanism 4 and a liquid path module 6 connected with the liquid suction and injection mechanism. And a centrifugal mechanism 2 is arranged in the constant-temperature centrifugal bin 3.
In the present invention, as shown in fig. 4 and 6, the thermostatic centrifugal cartridge 3 includes a double-layered casing 31, a heat insulating material 32, a heat conducting material 33, a condenser coil 34, a direct cooling type thermostat 35, and a plurality of shut-off valves 36.
Wherein, the section of the condenser coil 34 is hollow and the inside is communicated with the refrigerant 341. As shown in fig. 5, the condenser coil 34 is integrally formed in a spiral structure and surrounds the inside of the double-layered casing 31. The heat-conducting material 33 is filled in the gaps around the condenser coil 34, and the heat-insulating material 32 is arranged at the periphery.
In the invention, as the constant temperature centrifugal bin 3 is also provided with a double-layer shell in design, the side in contact with the outer layer is filled with heat insulation materials, and the conduction of heat to the outside of the centrifugal bin is weakened; the side contacted with the inner layer is filled with heat conducting materials in gaps around the condenser coil, so that heat conduction with the inner layer shell is enhanced, and further heat energy is better conducted inwards. Meanwhile, the design of the spiral condenser coil greatly increases the contact area between the spiral condenser coil and the inside of the centrifugal bin, and the temperature uniformity in the bin is better controlled. Therefore, the problem of nonuniform temperature and unstable temperature control in the centrifugal bin is effectively solved, the cell harvesting process can be kept in a constant temperature state all the time, and the cell harvesting effect is better.
Preferably, as shown in fig. 6, the direct cooling type thermostat 35 includes a thermostatic expansion valve 351, an evaporator 352, and a compressor 353. Wherein one end of the thermal expansion valve 351 is connected to one end of the condenser coil 34 and the other end is connected to one end of the evaporator 352 through one or both of the shut-off valves 36. The other end of the evaporator 352 is connected to one end of the compressor 353. The other end of the compressor 353 is connected to the other end of the condenser coil 34 via another one or two of the shut-off valves 36.
More preferably, a plurality of temperature probes 37 are also arranged in the constant temperature centrifugal bin 3. And the direct cooling type constant temperature device 35 further comprises an electric temperature control valve 354. The electric thermo valve 354 has one end connected between the thermal expansion valve 351 and the evaporator 352 and the other end connected between the compressor 353 and the other stop valve 36.
When the thermostatic centrifugal chamber 3 needs to be heated, the compressor 353 pressurizes the refrigerant 341 to be high-temperature and high-pressure gas, and then radiates heat on the condenser coil 34 after passing through the external shut-off valve 36, so that the thermostatic centrifugal chamber 3 is heated. The refrigerant 341 continues to pass through the external shut-off valve 36, the thermal expansion valve 351, and the evaporator 352 to become a low-temperature low-pressure liquid, and then circulates through the compressor 353. The constant temperature centrifugal bin 3 is also internally provided with a plurality of temperature probes 37, and the temperature of the refrigerant 341 can be regulated by controlling the electric temperature control valve 354 through a PID (proportion integration differentiation) regulating algorithm, so that the constant temperature effect in the constant temperature centrifugal bin 3 is achieved.
Therefore, the constant temperature centrifugal bin 3 adopts a heat pump technology, and a series of new technologies such as a spiral condenser coil pipe and the like are arranged in the interlayer of the centrifugal bin, so that the heat energy of a low-level heat source is transferred to a high-level heat source by applying the reverse Carnot circulation principle, the heat energy carrying capacity is strong, and the equivalent heating effect can be achieved only by about 1/4 of the energy consumption of the traditional heating mode.
The liquid sucking and injecting mechanism 4 comprises a rotary lifting module 41, a rotary rod 42, a liquid injecting station 43 and a liquid sucking station 44. The upper end of the rotary lifting module 41 is fixedly connected with the rotary rod 42. The two ends of the rotating rod 42 are respectively fixed with the liquid injection station 43 and the liquid suction station 44. The rotary lifting module 41 can drive the rotary rod 42 to realize up-and-down motion and rotary motion.
In the present invention, the specific structure of the rotation elevating module 41 is not limited, as long as it can drive the rotation rod 42 to realize up-and-down movement and rotation movement. For example, the rotary elevating module 41 includes a rotary motor and an upgrade cylinder to implement the rotation and elevating functions.
The liquid injection station 43 is used for realizing liquid injection of hypotonic liquid and fixed liquid. The priming station 43 includes a priming rotating motor 431, a priming rotating disk 432, a hypotonic priming needle 433 and a stationary priming needle 434. The liquid injection rotating motor 432 is used for driving the liquid injection rotating disc 432 to rotate. The hypotonic solution injection needle 433 and the fixed solution injection needle 434 are installed at intervals on the circumference of the injection turntable 432.
The pipetting station 44 is used to aspirate supernatant. The pipetting station 44 includes a pipetting carousel 441 and pipetting needles 442. The fluid sucking needles 442 are mounted on the circumference of the washing liquid rotary table 441.
In the invention, the liquid suction and injection mechanism 4 realizes high integration of the liquid suction and injection process, and solves the problems of huge harvesting system, complex control, high energy consumption and high production, use and maintenance cost in the past.
The centrifugal mechanism 2 comprises a rotary table 21, an adapter 22 with a swinging rod, a centrifugal motor 23 and a transmission shaft 24. The turntable 21 is provided with a plurality of adapters 22 with swing rods, and the lower side of the turntable 21 is connected with the centrifugal motor 23 through the transmission shaft 24. The turntable 21 can rotate under the drive of the centrifugal motor 23.
Wherein, a plurality of centrifuge tube cavities are arranged in each adapter 22 with the swing rod, for example, four centrifuge tube cavities are arranged, and each centrifuge tube cavity can be internally provided with a centrifuge tube. The hypotonic liquid injection needle 433 and the fixed liquid injection needle 434 are in one-to-one correspondence with the centrifuge tube cavities in the adapter 22 with swing rods. For example, two hypotonic injection needles 433 and two fixed injection needles 434 are provided on each of the injection turntables 432 to be spaced apart from each other. The aspiration needles 442 also have a one-to-one correspondence with centrifuge tube cavities in the swing link adapter 22. For example, four of the liquid sucking needles 442 are mounted on the periphery of the washing liquid rotary table 441.
The liquid path module 6 comprises a hypotonic liquid reagent bottle 61, a metering pump 63, a fixed liquid reagent bottle 64, a waste liquid collecting bottle 65, a distilled water bottle 67, a diaphragm pump 68 and a cleaning bin 69. Wherein the hypotonic reagent bottle 61 is connected to the hypotonic injection needle 433 through one of the metering pumps 63. The fixed liquid reagent bottle 64 is connected to the fixed liquid injection needle 434 via the other metering pump 63. The waste collection vial 65 is connected to the pipette needle 442 by one of the diaphragm pumps 68. The distilled water bottle 67 is connected to the washing chamber 69 via another diaphragm pump 68.
Preferably, a plurality of cleaning pipes are arranged in the cleaning bin 69 and are in one-to-one correspondence with the liquid sucking needles 442.
In addition, the fluid path module 6 may further include two-way valves 62 considering that the two hypotonic fluid injection needles 433 and the two fixed fluid injection needles 434 are mounted on the fluid injection carousel 432. The outlet of the hypotonic reagent bottle 61 is divided into two paths by one of the two-way valves 62, and is respectively connected with one of the metering pumps 63 and then is respectively connected with one of the hypotonic injection needles 433. The outlet of the fixed liquid reagent bottle 64 is divided into two paths by the other two-way valve 62, and is respectively connected with one metering pump 63 and then is respectively connected with one fixed liquid injection needle 433.
Meanwhile, considering that the four suction needles 442 are mounted on the suction turntable 441, the fluid path module 6 may further include a four-way valve 66. The inlet of the waste collection bottle 65 is connected to four of the aspiration needles 442 through the four-way valve 66.
The oscillating mechanism 5 has the functions of uniform mixing of lifting and eccentric oscillation. As shown in fig. 7, the oscillating mechanism 5 includes a fixed plate 51, a screw nut 52, a screw 53, a coupling 54, an oscillating lift motor 55, an oscillating motor 56, and an eccentric pallet 57. The lower end of the screw rod 53 is connected with an output shaft of the oscillating lifting motor 55 through the coupler 54. The lead screw nut 52 and the oscillating motor 56 are mounted on the fixed plate 51. The screw nut 52 is sleeved on the screw 53. The eccentric tray 57 is connected to an output shaft of the oscillating motor 56.
Thereby, the oscillating motor 55 can be lifted and lowered by the oscillating motor 56 and the eccentric tray 57 by the rotation of the screw 53. The oscillating motor 56 may rotate the eccentric tray 57. In this way, the oscillating mechanism 5 can rise in the liquid injection process, so that the eccentric tray 57 holds the bottom of the adapter 22 with the oscillating bar, and further the oscillating motor 56 can drive the adapter 22 with the oscillating bar to perform eccentric oscillation and shaking through the eccentric tray 57, thereby achieving the purpose of fully mixing the reagents in the centrifuge tube.
In the present invention, preferably, a centrifugal bin sealing plate 11 is further fixed to the platen 1. The centrifugal bin sealing plate 11 is used for sealing an upper end opening of the constant-temperature centrifugal bin 3. The centrifugal bin sealing plate 11 is provided with a centrifugal bin door 12. Through the centrifugal bin gate 12, it is convenient to view the internal structure of the thermostatic centrifugal bin.
The cell harvesting system of the present invention is used in a manner similar to the methods used in existing cell harvesting systems. The cell harvesting system is first turned on. After the cell harvesting system is started, the constant temperature centrifugal bin 3 is automatically heated to a preset temperature, and then a centrifuge tube sample is manually placed into the adapter 22 with the swing rod, and the centrifugal bin door 12 is closed. And then filling hypotonic liquid, filling fixing liquid, shaking and mixing uniformly, centrifuging, sucking supernatant and the like in sequence according to the harvesting process flow. When filling hypotonic liquid, the liquid filling station 43 of the liquid sucking and filling mechanism 4 rotates to the position above the corresponding adapter 22 with the swing rod, after part of samples are filled, the liquid filling station rotates to another position through the liquid filling rotating motor 431, filling of residual samples of the adapter is completed, and then the centrifugal motor 23 rotates to fill other adapters, so that the filling and fixing liquid flows are the same as above. When the supernatant is required to be sucked, the rotating rod 42 is driven to ascend and rotate by rotating the lifting module 41, so that the liquid suction station 44 rotates to the position above the corresponding adapter 22 with the swinging rod, and then the lifting module 41 is rotated to descend, so that the supernatant sucking process is completed. After sucking the supernatant, the pipetting station 44 moves into the washing chamber 69, the diaphragm pump 68 supplies water into the washing chamber 69, and the pipetting needle 442 performs pipetting a plurality of times to complete the washing process. After the above procedure is repeated for a plurality of times, cell harvesting is completed, the centrifugal bin gate 12 is opened, and a centrifuge tube sample is taken out.
The cell harvesting system enables the constant temperature control level in the cell harvesting process to take a brand-new step, and can further improve the cell harvesting effect, chromosome quantity and quality; meanwhile, the integrated design of liquid suction and injection simplifies the structure, reduces the cost and enables the harvesting system to be more miniaturized.
The above examples of the present invention are merely illustrative of the present invention and are not intended to limit the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. Not all embodiments are exhaustive. All obvious changes or modifications which come within the spirit of the invention are desired to be protected.
Claims (1)
1. A cell harvesting system; the centrifugal machine comprises a bedplate (1), wherein a constant-temperature centrifugal bin (3) and an oscillating mechanism (5) are arranged below the bedplate (1); a liquid suction and injection mechanism (4) and a liquid path module (6) connected with the liquid suction and injection mechanism are arranged on the bedplate (1); a centrifugal mechanism (2) is arranged in the constant-temperature centrifugal bin (3), and is characterized in that; the constant temperature centrifugal bin (3) comprises a double-layer shell (31), a heat insulation material (32), a heat conduction material (33), a condenser coil (34), a direct cooling type constant temperature device (35) and a plurality of stop valves (36); the section of the condenser coil (34) is of a hollow structure, and the inside of the condenser coil is communicated with refrigerant (341); the condenser coil (34) is integrally of a spiral structure and surrounds the inside of the double-layer shell (31); the gaps around the condenser coil (34) are filled with the heat conducting material (33), and the periphery of the condenser coil is provided with the heat insulating material (32); the direct cooling type constant temperature device (35) comprises a thermal expansion valve (351), an evaporator (352) and a compressor (353); one end of the thermal expansion valve (351) is connected with one end of the condenser coil (34) through one of the stop valves (36), and the other end of the thermal expansion valve is connected with one end of the evaporator (352); the other end of the evaporator (352) is connected with one end of the compressor (353); the other end of the compressor (353) is connected with the other end of the condenser coil (34) through another stop valve (36); a plurality of temperature probes (37) are also arranged in the constant temperature centrifugal bin (3); and the direct cooling type constant temperature device (35) further comprises an electric temperature control valve (354); one end of the electric temperature control valve (354) is connected between the thermal expansion valve (351) and the evaporator (352), and the other end is connected between the compressor (353) and the other stop valve (36); the liquid sucking and injecting mechanism (4) comprises a rotary lifting module (41), a rotary rod (42), a liquid injecting station (43) and a liquid sucking station (44); the upper end of the rotary lifting module (41) is fixedly connected with the rotary rod (42); the two ends of the rotating rod (42) are respectively fixed with the liquid injection station (43) and the liquid suction station (44); the rotary lifting module (41) can drive the rotary rod (42) to realize up-and-down motion and rotary motion; the liquid injection station (43) comprises a liquid injection rotating motor (431), a liquid injection rotating disc (432), a hypotonic liquid injection needle (433) and a fixed liquid injection needle (434); the liquid injection rotating motor (431) is used for driving the liquid injection rotating disc (432) to rotate; the hypotonic liquid injection needle (433) and the fixed liquid injection needle (434) are arranged on the circumference of the liquid injection turntable (432) at intervals; the liquid sucking station (44) comprises a liquid sucking rotary disc (441) and liquid sucking needles (442), and the liquid sucking needles (442) are arranged on the circumference of the liquid sucking rotary disc (441); the centrifugal mechanism (2) comprises a turntable (21), an adapter (22) with a swing rod, a centrifugal motor (23) and a transmission shaft (24); a plurality of adapters (22) with swing rods are arranged on the turntable (21), and the lower side of the turntable (21) is connected with the centrifugal motor (23) through the transmission shaft (24); a plurality of centrifuge tube cavities are arranged in each adapter (22) with the swing rod; the hypotonic liquid injection needle (433) and the fixed liquid injection needle (434) are in one-to-one correspondence with the centrifuge tube holes in the adapter (22) with the swing rod; the liquid sucking needles (442) are also in one-to-one correspondence with centrifuge tube cavities in the adapter (22) with the swing rod; the liquid path module (6) comprises a hypotonic liquid reagent bottle (61), a metering pump (63), a fixed liquid reagent bottle (64), a waste liquid collecting bottle (65), a distilled water bottle (67), a diaphragm pump (68) and a cleaning bin (69); the hypotonic solution reagent bottle (61) is connected with the hypotonic solution injection needle (433) through one of the metering pumps (63); the fixed liquid reagent bottle (64) is connected with the fixed liquid injection needle (434) through the other metering pump (63); the waste liquid collecting bottle (65) is connected with the liquid sucking needle (442) through one of the diaphragm pumps (68); the distilled water bottle (67) is connected with the cleaning bin (69) through another diaphragm pump (68); the oscillating mechanism (5) comprises a fixed plate (51), a screw rod nut (52), a screw rod (53), a coupler (54), an oscillating lifting motor (55), an oscillating motor (56) and an eccentric tray (57); the lower end of the screw rod (53) is connected with an output shaft of the oscillating lifting motor (55) through the coupler (54); the screw nut (52) and the oscillating motor (56) are arranged on the fixed plate (51); the screw rod nut (52) is sleeved on the screw rod (53); the eccentric tray (57) is connected with an output shaft of the oscillating motor (56).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN209117705U (en) * | 2018-11-28 | 2019-07-16 | 佛山市艾达思精密仪器有限公司 | A kind of harvest processing system for full-automatic chromosome harvest equipment |
| CN112066597A (en) * | 2020-09-18 | 2020-12-11 | 广州言几方科技有限公司 | Efficient energy-saving heat pump utilizing low-grade heat energy |
| CN112410209A (en) * | 2020-11-26 | 2021-02-26 | 广州达晖生物技术股份有限公司 | Temperature control system for chromosome harvesting equipment and automatic temperature control method |
| CN112816720A (en) * | 2020-12-31 | 2021-05-18 | 上海北昂医药科技股份有限公司 | Full-automatic cell harvesting instrument of integrated liquid path system |
| CN214160072U (en) * | 2020-11-26 | 2021-09-10 | 广州达晖生物技术股份有限公司 | Chromosome harvesting equipment |
| WO2022143578A1 (en) * | 2020-12-28 | 2022-07-07 | 南京金斯瑞生物科技有限公司 | Biological sample processing system and processing method |
| CN115044960A (en) * | 2022-04-14 | 2022-09-13 | 哈尔滨工业大学 | Rotary oscillation high-temperature furnace |
-
2022
- 2022-12-29 CN CN202211708015.4A patent/CN115873707B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN209117705U (en) * | 2018-11-28 | 2019-07-16 | 佛山市艾达思精密仪器有限公司 | A kind of harvest processing system for full-automatic chromosome harvest equipment |
| CN112066597A (en) * | 2020-09-18 | 2020-12-11 | 广州言几方科技有限公司 | Efficient energy-saving heat pump utilizing low-grade heat energy |
| CN112410209A (en) * | 2020-11-26 | 2021-02-26 | 广州达晖生物技术股份有限公司 | Temperature control system for chromosome harvesting equipment and automatic temperature control method |
| CN214160072U (en) * | 2020-11-26 | 2021-09-10 | 广州达晖生物技术股份有限公司 | Chromosome harvesting equipment |
| WO2022143578A1 (en) * | 2020-12-28 | 2022-07-07 | 南京金斯瑞生物科技有限公司 | Biological sample processing system and processing method |
| CN112816720A (en) * | 2020-12-31 | 2021-05-18 | 上海北昂医药科技股份有限公司 | Full-automatic cell harvesting instrument of integrated liquid path system |
| CN115044960A (en) * | 2022-04-14 | 2022-09-13 | 哈尔滨工业大学 | Rotary oscillation high-temperature furnace |
Non-Patent Citations (1)
| Title |
|---|
| 袁伯俊 北京：军事医学科学出版社.新药临床前安全性评价与实践.1997,第98-99页. * |
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