CN115869471B - Anticoagulation functional material, preparation method and application thereof - Google Patents
Anticoagulation functional material, preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an anticoagulant functional material, a preparation method and application thereof. The preparation method comprises the steps of firstly, preprocessing medical metal to generate suspension bonds on the surface of the medical metal; and then soaking the pretreated medical metal in a base solution and/or a hydrophilic coating solution, and forming a hydrophilic coating on the surface of the medical metal by regulating and controlling the temperature and the soaking time of the soaking solution. The anticoagulation functional material prepared by the method has excellent anticoagulation function, can be used as an implantation material for disease treatment, and can effectively avoid thrombosis. And different from the traditional drug-loaded coating system, the anticoagulant molecule fixed on the surface of the substrate is more stable through chemical actions such as self-assembly, covalent grafting and the like, the long-term hydrophilic service characteristic of the surface of the material can be realized, and the drug release kinetics is not involved, so that the method is simpler and more effective.
Description
Technical Field
The invention belongs to the technical field of anticoagulation materials, and particularly relates to an anticoagulation functional material, a preparation method and application thereof.
Background
The treatment of aneurysms, especially cerebral aneurysms, is currently the mainstream of treatment by implanting a dense-mesh stent by a nerve intervention method, the dense-mesh stent can obviously reduce the flow rate and flow of blood entering the aneurysms by applying the principle of blood flow dynamics, thrombus is gradually formed in the aneurysms, and then the thrombus is eliminated by blood metabolism to reconstruct blood vessels at lesion sites, so that the dense-mesh stent has the advantages of high safety, wide application range, convenience in operation and the like. However, after the dense net stent is used as a foreign body to be implanted into an arterial vessel, immune response of relevant tissues of a human body can be caused, and acute coagulation response is the most unfavorable for treating aneurysms, thrombus and even blood clots can be formed on the surface and inside of the dense net stent, so that the dense net stent is blocked, blood supply of a brain is affected, and risks such as cerebral infarction are caused. The current main practice is that patients take double-resistant drugs after operation, which not only increases postoperative complications, but also is a huge psychological pressure and economic burden for patients.
Therefore, the surface function of dense mesh scaffolds is modified to improve their anticoagulant properties, and reducing the risk of thrombosis is the focus of research. Currently, aiming at anticoagulation modification of a dense net stent, for example, an anticoagulation small molecule function modification layer is constructed on the surface of the dense net stent; the construction of hydrophilic polymer coating improves the anticoagulation capacity of the stent surface. However, there are still problems of less researches, insignificant modification effects, etc.
Disclosure of Invention
Aiming at the prior art, the invention provides an anticoagulation functional material, a preparation method and application thereof, so as to solve the problem of poor anticoagulation effect of the existing dense net stent.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: providing an anticoagulant functional material, which is prepared by the following steps:
s1: pretreating medical metal to generate suspension bonds on the surface of the medical metal;
s2: soaking the pretreated medical metal in a base solution and/or a hydrophilic coating solution, and regulating and controlling the temperature and the soaking time of the soaking solution to form a hydrophilic coating on the surface of the medical metal.
The beneficial effects of the invention adopting the technical scheme are as follows: the medical metal is pretreated, a large number of active groups are exposed on the surface of the medical metal after the treatment, the active groups are connected with a coating matrix in a hydrophilic coating solution in a covalent grafting mode, meanwhile, the coating matrix forms a hydrophilic coating on the surface of the medical metal in a self-assembly mode, and the hydrophilic coating is firmly attached to the surface of the medical metal, so that the stability of the hydrophilic coating can be ensured even under long-time flushing of blood flow. The hydrophilic coating has good hydrophilic performance and biocompatibility, can reduce or inhibit adhesion and activation of different components of blood such as fibrinogen, platelets and the like, further reduces thrombus formation, and has good anticoagulation effect.
On the basis of the technical scheme, the invention can be improved as follows.
Further, the pretreatment of the medical metal comprises the following steps:
SS1: sequentially cleaning medical metal with ethanol and deionized water;
SS2: immersing medical metal treated by SS1 in alkali solution with the concentration of 0.01-0.1 g/mL and the temperature of 50-60 ℃ for 90-150 min, carrying out ultrasonic oscillation once every 10min in the immersing process, and irradiating the standing horse with ultraviolet light for 5-10 min after ultrasonic oscillation; the irradiation intensity of the ultraviolet light is 100-200 mu W/cm 2 。
The invention adopts the further technical proposal and has the beneficial effects that: according to the invention, the medical metal is pretreated by the alkali liquor, the surface of the medical metal is etched by the alkali liquor, the surface of the medical metal is passivated, active groups such as hydroxyl groups are introduced to the surface of the medical metal in the etching process, and suspension bonds are generated on the surface of the medical metal, so that the adhesion of a hydrophilic coating matrix on the surface of the medical metal can be promoted, and the adhesion stability of the hydrophilic coating can be improved.
In the etching process, ultrasonic oscillation is regularly carried out, the ultrasonic can accelerate the etching of alkali liquor, the etching is more uniform, and the arrangement of suspension bonds generated on the surface of medical metal is more regular. The irradiation of the ultrasonic back-up Ma Yongzi ultraviolet rays can influence the movement of molecular atoms on the metal surface by the irradiation of the ultraviolet rays, so that the etched structure is solidified, the formation of a regular structure is facilitated, the number of active groups on the medical metal surface can be increased, and the grafting of hydrophilic substances is facilitated.
Further, the alkali liquor is sodium hydroxide solution; the ultrasonic intensity is 60-80 KHz, and the duration of each ultrasonic is 2-5 min.
Further, the hydrophilic coating is obtained by immersing in a base liquid and a hydrophilic coating solution; the base solution is prepared through the following steps:
SS1: taking absolute ethyl alcohol, and regulating the pH value of the absolute ethyl alcohol to 4.5-5.5 by glacial acetic acid;
SS2: mixing the absolute ethyl alcohol with the pH value adjusted and the silane coupling agent according to the mass ratio of 1:0.15-0.2, and then hydrolyzing for 2 hours at room temperature to obtain the modified aqueous solution;
the hydrophilic coating solution is polyethylene glycol solution.
Further, the pretreated medical metal is soaked in a base solution and a hydrophilic coating solution in sequence; the soaking time in the base solution is 3-5 min; curing for 45-60 min at 75-85 ℃ after soaking in the substrate liquid, and then soaking in the hydrophilic coating solution for 2-3 h.
Further, the pretreatment of the medical metal comprises the following steps:
SS1: polishing the medical metal, and then washing cleanly;
SS2: NH treatment of SS1 treated medical metals in Hydrogen atmosphere 3 Etching at 600-800 deg.c and NH 3 The flow is 1000-1200 sccm, and the etching time is 10-20 min.
Further, the hydrophilic coating is obtained by soaking in a hydrophilic coating solution; the hydrophilic coating solution is prepared through the following steps:
SS1: 2 to 5 parts of silane coupling agent and 5 to 10 parts of dopamine are dissolved in 100 parts of absolute ethyl alcohol together to form solution A;
SS2: 2-5 parts of coating matrix is dissolved in 100 parts of absolute ethyl alcohol to form solution B; the coating matrix is at least one of phosphorylcholine, hyaluronic acid and tannic acid;
SS3: adding a molecular sieve into the solution B, and dropwise adding the solution B into the solution A under the stirring condition to obtain the molecular sieve; the mass ratio of the added molecular sieve to the coating matrix is 1:4-6;
the parts are mass parts.
The beneficial effects of the invention adopting the technical scheme are as follows: molecular sieves are added into the hydrophilic coating solution, so that the dehydration reaction is facilitated, the grafting rate of solutes in the hydrophilic coating solution on medical metals can be improved, the stability of the coating is better, and the hydrophilic performance is also obviously improved.
Further, the soaking time of the pretreated medical metal in the hydrophilic coating solution is 2-3 hours.
The beneficial effects of the invention are as follows:
the anticoagulation functional material prepared by the method has excellent anticoagulation function, can be used as an implantation material for disease treatment, and can effectively avoid thrombosis. And different from the traditional drug-loaded coating system, the invention makes anticoagulant molecules fixed on the surface of the substrate more stable through chemical actions such as self-assembly, covalent grafting and the like, can realize the long-term hydrophilic service characteristic of the surface of the material, and does not relate to drug release kinetics, thus being simpler and more effective.
Drawings
FIG. 1 shows the results of the measurement of the included hydrophilic angle of the anticoagulation functional material in comparative example 1 and example 1;
FIG. 2 shows the results of the measurement of the included hydrophilic angle of the anticoagulation functional material in comparative example 2 and example 4;
FIG. 3 shows the platelet adhesion effect of the anticoagulation functional material in comparative example 1 and example 1;
FIG. 4 shows the platelet adhesion effect of the anticoagulation functional material in comparative example 2 and example 4;
FIG. 5 is a fluorescent staining effect of the anticoagulation functional material in comparative example 1 and example 1;
FIG. 6 shows the effect of fluorescent staining of the anticoagulation functional material in comparative example 2 and example 4.
Detailed Description
The following describes the present invention in detail with reference to examples.
Example 1
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Pretreatment of medical metal substrates: and (3) taking medical nickel-titanium alloy, cleaning the medical nickel-titanium alloy with ethanol and deionized water for 3 times respectively, and then blowing off surface moisture. Then the cleaned nickel-titanium alloy is put into sodium hydroxide solution with the concentration of 0.05g/mL and the temperature of 55 ℃ for soaking for 120min; in the soaking process, the ultrasonic oscillation is carried out every 10min, the ultrasonic intensity is 70KHz, and the duration of each ultrasonic is 4min; and irradiating the standing horse with ultraviolet light for 10min after ultrasonic oscillation, wherein the irradiation intensity of ultraviolet light is 150 μW/cm 2 。
(2) Attaching a hydrophilic coating:
s1: taking absolute ethyl alcohol, and regulating the pH value of the absolute ethyl alcohol to about 5.0 by glacial acetic acid; then mixing the absolute ethyl alcohol with the pH value adjusted and the silane coupling agent APTES according to the mass ratio of 1:0.15, ultrasonically oscillating for 10min with the power of 40KHz, and hydrolyzing for 2h at room temperature to obtain a substrate liquid;
s2: soaking the pretreated nickel-titanium alloy in a base solution for 4min, and then taking out and putting into an oven at 80 ℃ for curing for 1h;
s3: preparing PEG-600 into a 50wt% solution, then placing the nickel-titanium alloy subjected to S2 treatment into a polyethylene glycol solution, soaking for 2 hours at room temperature, taking out, washing with deionized water for 3 times, and drying surface moisture at 50 ℃ to obtain the nickel-titanium alloy.
Example 2
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Medical metal substratePretreatment: and (3) taking medical nickel-titanium alloy, cleaning the medical nickel-titanium alloy with ethanol and deionized water for 3 times respectively, and then blowing off surface moisture. Then the cleaned nickel-titanium alloy is put into sodium hydroxide solution with the concentration of 0.1g/mL and the temperature of 50 ℃ for soaking for 90min; in the soaking process, the ultrasonic oscillation is carried out every 10min, the ultrasonic intensity is 60KHz, and the duration of each ultrasonic is 5min; and irradiating the standing horse with ultraviolet light for 10min after ultrasonic oscillation, wherein the irradiation intensity of ultraviolet light is 100 μW/cm 2 。
(2) Attaching a hydrophilic coating:
s1: taking absolute ethyl alcohol, and regulating the pH value of the absolute ethyl alcohol to about 4.5 by glacial acetic acid; then mixing the absolute ethyl alcohol with the pH value adjusted and the silane coupling agent APTES according to the mass ratio of 1:0.2, ultrasonically oscillating for 10min with the power of 40KHz, and hydrolyzing for 2h at room temperature to obtain a substrate liquid;
s2: soaking the pretreated nickel-titanium alloy in a base solution for 5min, and then taking out and putting into an oven at 80 ℃ for curing for 1h;
s3: preparing PEG-600 into a 50wt% solution, then placing the nickel-titanium alloy subjected to S2 treatment into a polyethylene glycol solution, soaking for 2 hours at room temperature, taking out, washing with deionized water for 3 times, and drying surface moisture at 50 ℃ to obtain the nickel-titanium alloy.
Example 3
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Pretreatment of medical metal substrates: and (3) taking medical nickel-titanium alloy, cleaning the medical nickel-titanium alloy with ethanol and deionized water for 3 times respectively, and then blowing off surface moisture. Then the cleaned nickel-titanium alloy is put into sodium hydroxide solution with the concentration of 0.01g/mL and the temperature of 60 ℃ to be soaked for 120min; in the soaking process, the ultrasonic oscillation is carried out every 10min, the ultrasonic intensity is 80KHz, and the duration of each ultrasonic is 2min; and irradiating the standing horse with ultraviolet light for 5min after ultrasonic oscillation, wherein the irradiation intensity of ultraviolet light is 200 mu W/cm 2 。
(2) Attaching a hydrophilic coating:
s1: taking absolute ethyl alcohol, and regulating the pH value of the absolute ethyl alcohol to about 5.5 by glacial acetic acid; then mixing the absolute ethyl alcohol with the pH value adjusted and the silane coupling agent APTES according to the mass ratio of 1:0.2, ultrasonically oscillating for 10min with the power of 40KHz, and hydrolyzing for 2h at room temperature to obtain a substrate liquid;
s2: soaking the pretreated nickel-titanium alloy in a base solution for 3min, and then taking out and putting into an oven at 80 ℃ for curing for 1h;
s3: preparing PEG-600 into a 50wt% solution, then placing the nickel-titanium alloy subjected to S2 treatment into a polyethylene glycol solution, soaking for 3 hours at room temperature, taking out, washing with deionized water for 3 times, and drying surface moisture at 60 ℃ to obtain the nickel-titanium alloy.
Example 4
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Pretreatment of medical metal substrates: the surface of the medical nickel-titanium alloy is firstly polished, then is respectively washed for 3 times by ethanol and deionized water, and then is dried by blowing. Then NH is carried out on the cleaned nickel-titanium alloy in the hydrogen atmosphere 3 Etching, wherein the etching temperature is 650 ℃, NH 3 The flow rate was 1000sccm and the etching time was 15min.
(2) Attaching a hydrophilic coating:
s1: 4 parts by mass of silane coupling agent APTES and 8 parts by mass of dopamine are dissolved in 100 parts by mass of absolute ethyl alcohol to form solution A; dissolving 4 parts by mass of a coating matrix in 100 parts by mass of absolute ethyl alcohol to form a solution B, wherein the coating matrix is a mixture formed by mixing phosphorylcholine, hyaluronic acid and tannic acid according to a mass ratio of 10:5:2; adding a 3A molecular sieve with the particle size of 10mm into the solution B, and dropwise adding the solution B into the solution A under the stirring condition to obtain a hydrophilic coating solution, wherein the mass ratio of the added molecular sieve to the coating matrix is 1:5;
s2: and (3) putting the pretreated nickel-titanium alloy into a hydrophilic coating solution, soaking for 3 hours at room temperature, taking out, washing for 3 times by deionized water, and drying the surface moisture at 50 ℃ to obtain the nickel-titanium alloy.
Example 5
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Pretreatment of medical metal substrates: the surface of the medical nickel-titanium alloy is firstly polished, then is respectively washed for 3 times by ethanol and deionized water, and then is dried by blowing. Then NH is carried out on the cleaned nickel-titanium alloy in the hydrogen atmosphere 3 Etching, wherein the etching temperature is 600 ℃, NH 3 The flow rate was 1200sccm and the etching time was 20min.
(2) Attaching a hydrophilic coating:
s1: 2 parts by mass of silane coupling agent APTES and 5 parts by mass of dopamine are dissolved in 100 parts by mass of absolute ethyl alcohol to form solution A; 2 parts by mass of a coating matrix is dissolved in 100 parts by mass of absolute ethyl alcohol to form a solution B, wherein the coating matrix is a mixture formed by mixing phosphorylcholine, hyaluronic acid and tannic acid according to a mass ratio of 10:5:2; adding a 3A molecular sieve with the particle size of 10mm into the solution B, and dropwise adding the solution B into the solution A under the stirring condition to obtain a hydrophilic coating solution, wherein the mass ratio of the added molecular sieve to the coating matrix is 1:4;
s2: and (3) putting the pretreated nickel-titanium alloy into a hydrophilic coating solution, soaking for 2 hours at room temperature, taking out, washing for 3 times by deionized water, and drying the surface moisture at 60 ℃ to obtain the nickel-titanium alloy.
Example 6
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. The preparation method comprises the following steps:
(1) Pretreatment of medical metal substrates: the surface of the medical nickel-titanium alloy is firstly polished, then is respectively washed for 3 times by ethanol and deionized water, and then is dried by blowing. Then NH is carried out on the cleaned nickel-titanium alloy in the hydrogen atmosphere 3 Etching at 800 deg.c and NH 3 The flow rate was 1000sccm and the etching time was 10min.
(2) Attaching a hydrophilic coating:
s1: 5 parts by mass of silane coupling agent APTES and 10 parts by mass of dopamine are dissolved in 100 parts by mass of absolute ethyl alcohol to form solution A; dissolving 5 parts by mass of a coating matrix in 100 parts by mass of absolute ethyl alcohol to form a solution B, wherein the coating matrix is a mixture formed by mixing phosphorylcholine, hyaluronic acid and tannic acid according to a mass ratio of 10:5:2; adding a 3A molecular sieve with the particle size of 10mm into the solution B, and dropwise adding the solution B into the solution A under the stirring condition to obtain a hydrophilic coating solution, wherein the mass ratio of the added molecular sieve to a coating matrix is 1:6;
s2: and (3) putting the pretreated nickel-titanium alloy into a hydrophilic coating solution, soaking for 3 hours at room temperature, taking out, washing for 3 times by deionized water, and drying the surface moisture at 60 ℃ to obtain the nickel-titanium alloy.
Comparative example 1
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. In comparison with example 1, when the medical metal substrate was pretreated, only alkali lye immersion was performed, ultrasonic oscillation was not performed during the immersion, and ultraviolet irradiation was not performed, and the rest of the operations were the same as in example 1.
Comparative example 2
An anticoagulant functional material comprises a medical metal substrate and a hydrophilic coating attached to the medical metal substrate. In contrast to example 4, no molecular sieve was added to the hydrophilic coating solution, and the rest of the procedure was the same as in example 4.
Analysis of results
The surface hydrophilicity and hydrophobicity of the anticoagulation functional materials prepared in examples 1 and 4 and comparative examples 1 and 2 were demonstrated by UP water drops, and the results are shown in fig. 1 and 2; wherein, fig. 1 (a) shows the results of detecting the hydrophilic included angle of the anticoagulation functional material in comparative example 1, and fig. 1 (b) shows the results of detecting the hydrophilic included angle of the anticoagulation functional material in example 1; fig. 2 (a) shows the results of the measurement of the hydrophilic angle of the anticoagulation material in comparative example 2, and fig. 2 (b) shows the results of the measurement of the hydrophilic angle of the anticoagulation material in example 4.
As can be seen from FIG. 1, the pretreatment modes can cause larger difference in the hydrophilic and hydrophobic properties of the anticoagulant functional material, and the pretreatment modes in the invention can not only enable the hydrophilic coating to be more firmly attached to medical metal, but also increase the hydrophilic grafting rate of the hydrophilic coating, and improve the hydrophilic property of the hydrophilic coating. As can be seen from fig. 2, the addition of the molecular sieve to the hydrophilic coating solution can significantly improve the hydrophilic performance of the hydrophilic coating, because the molecular sieve can improve the hydrophilic grafting rate after being added, thereby making the hydrophilic performance more excellent.
Experimental example: observation of platelet adhesion morphology and activation Using immunofluorescent staining and scanning Electron microscopy
Blood used in the experimental examples was donated to the unknown volunteer. Mixing fresh blood with 3.8% sodium citrate at a ratio of 10:1, centrifuging on a centrifuge for 15min at 1500rpm, and collecting supernatant to obtain rich plate slurry (PRP);
placing the anticoagulation functional materials prepared in the examples 1 and 4 and the comparative examples 1 and 2 in 24 pore plates respectively, dripping 100 mu L of rich plate pulp on the surface of each sample, and placing the mixture in a constant temperature incubator at 37 ℃ for incubation for 1h;
the incubated samples were removed, washed three times with 0.9% sodium chloride and fixed with 2.5% glutaraldehyde for 4h.
The immunofluorescence staining steps are as follows:
s1: washing the sample after 3 times of fixation with 0.9% sodium chloride;
s2: 70 mu L of Rhodamine (rhodomine-phalloidin) solution is dripped on the surface of each sample, and the sample is placed for 15min under the dark condition;
s3: the stained sample was washed 3 times with 0.9% sodium chloride and dried and observed under a fluorescence microscope.
Scanning and observing platelets:
s1: the above samples were dehydrated: sequentially placing the samples in 50%,75%,90% and 100% alcohol solutions for 15min each time;
s2: dealcoholization: sequentially placing the samples in 50%,75%,90% and 100% isoamyl acetate solutions for 15min each time;
s3: and (5) drying the critical point, performing metal spraying treatment, and observing through a scanning electron microscope.
The platelet adhesion scanning electron microscopy results are shown in FIGS. 3 and 4 (detection scale 100 μm); wherein, fig. 3 (a) shows the platelet adhesion effect of the anticoagulation functional material in comparative example 1, and fig. 3 (b) shows the platelet adhesion effect of the anticoagulation functional material in example 1; fig. 4 (a) shows the platelet adhesion effect of the anticoagulation functional material in comparative example 2, and fig. 4 (b) shows the platelet adhesion effect of the anticoagulation functional material in example 4.
As can be seen from fig. 3 and 4, the anticoagulant functional material prepared by the method of the present invention has less platelet adhesion, has more excellent anticoagulant property, and can effectively avoid thrombosis after being implanted into a body.
Platelet adhesion fluorescence staining is shown in fig. 5 and 6; wherein, fig. 5 (a) shows the fluorescent staining effect of the anticoagulation functional material in comparative example 1, and fig. 3 (b) shows the fluorescent staining effect of the anticoagulation functional material in example 1; fig. 6 (a) shows the effect of fluorescent staining of the anticoagulation functional material in comparative example 2, and fig. 6 (b) shows the effect of fluorescent staining of the anticoagulation functional material in example 4.
As can be seen from fig. 5 and 6, the surface of the anticoagulation functional material prepared by the method has little or no adhesion of platelets, and has better anticoagulation performance as compared with the result of a platelet adhesion scanning electron microscope, and thrombus can be effectively avoided after the anticoagulation functional material is implanted into a human body.
While specific embodiments of the invention have been described in detail in connection with the examples, it should not be construed as limiting the scope of protection of the patent. Various modifications and variations which may be made by those skilled in the art without the creative effort are within the scope of the patent described in the claims.
Claims (4)
1. The preparation method of the anticoagulation functional material is characterized by comprising the following steps of:
s1: pretreating medical metal to generate suspension bonds on the surface of the medical metal; the pretreatment of the medical metal comprises the following steps:
SS1: polishing the medical metal, and then washing cleanly;
SS2: after treatment of SS1 in hydrogen atmosphereNH of medical metal 3 Etching, wherein the etching temperature is 600-800 ℃, and NH is performed 3 The flow is 1000-1200 sccm, and the etching time is 10-20 min;
s2: soaking the pretreated medical metal in a hydrophilic coating solution, and regulating and controlling the temperature and the soaking time of the soaking solution to form a hydrophilic coating on the surface of the medical metal; the hydrophilic coating is obtained by soaking in a hydrophilic coating solution; the hydrophilic coating solution is prepared through the following steps:
SS1: 2-5 parts of silane coupling agent and 5-10 parts of dopamine are dissolved in 100 parts of absolute ethyl alcohol together to form a solution A;
SS2: 2-5 parts of coating matrix is dissolved in 100 parts of absolute ethyl alcohol to form a solution B; the coating matrix is at least one of phosphorylcholine, hyaluronic acid and tannic acid;
SS3: adding a molecular sieve into the solution B, and dropwise adding the solution B into the solution A under the stirring condition to obtain the molecular sieve; the mass ratio of the added molecular sieve to the coating matrix is 1:4-6;
the parts are mass parts.
2. The method of manufacturing according to claim 1, characterized in that: the soaking time of the pretreated medical metal in the hydrophilic coating solution is 2-3 hours.
3. An anticoagulant functional material prepared by the preparation method of any one of claims 1 to 2.
4. Use of an anticoagulant functional material according to claim 3 for the preparation of a dense mesh scaffold.
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