CN115856134B - Detection method and device for determining sugar content in human urine based on liquid chromatography - Google Patents
Detection method and device for determining sugar content in human urine based on liquid chromatography Download PDFInfo
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Abstract
The invention relates to urine detection technology, and discloses a detection method for determining sugar content in human urine based on liquid chromatography, which comprises the following steps: performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, detecting components of the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample; dissolving the centrifugal urine sample by using a dissolving reagent to obtain a dissolved urine sample, extracting characteristics of the dissolved urine sample to obtain characteristics of the urine sample, preparing a stationary phase and a mobile phase of the dissolved urine sample, calculating a dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolved urine sample; filling the chromatographic column to obtain a filled chromatographic column, and performing chromatographic analysis on the dissolved urine sample by using the filled chromatographic column to obtain a urine sample chromatogram; and (3) scheduling a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer, and analyzing the sugar content in the dissolved urine sample. The invention aims to improve the detection accuracy of measuring the sugar content in human urine.
Description
Technical Field
The invention relates to the technical field of urine detection, in particular to a detection method and device for determining sugar content in human urine based on liquid chromatography.
Background
Urine detection is a mode for detecting urine in medicine, and comprises urine routine analysis, urine component detection (such as urine red blood cells, white blood cells and the like), protein component quantitative determination, urine enzyme determination and the like, and has important value for clinical diagnosis, judging curative effect and prognosis, for example, the urine sugar content detection is mainly adopted currently by combining liquid chromatography-tandem mass spectrometry to detect the sugar content in urine, and the sugar content is determined once by the color represented by the liquid chromatography and the substances detected by a mass spectrometer, but the liquid chromatography and the mass spectrometer in the method lack connectivity, so that the accuracy of the urine sugar content detection is reduced, and therefore, a method capable of improving the detection accuracy of the sugar content in urine of a person is needed.
Disclosure of Invention
The invention provides a detection method and a detection device for determining sugar content in human urine based on liquid chromatography, and mainly aims to improve the detection accuracy of determining the sugar content in human urine.
In order to achieve the above object, the present invention provides a detection method for determining sugar content in human urine based on liquid chromatography, comprising:
obtaining a urine sample to be tested, performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, performing component detection on the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample components;
performing dissolution treatment on the centrifugal urine sample by using the dissolution reagent to obtain a dissolution urine sample, performing feature extraction on the dissolution urine sample to obtain urine sample features, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample features, calculating a dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolution urine sample according to the dissolution coefficient;
filling the chromatographic column with the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram;
and dispatching a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer according to the mass spectrum sequence, and analyzing the sugar content in the dissolved urine sample according to the sugar-containing substances.
Optionally, the detecting the components of the centrifuged urine sample to obtain urine sample components comprises:
sampling the centrifugal urine sample to obtain a urine sample, and carrying out element analysis on the urine sample to obtain analysis elements;
combining each element in the analysis elements according to a preset element combination table to form combined elements;
inquiring a detection reagent corresponding to the combined element, and detecting the urine sample by using the detection reagent to obtain a detection result;
screening the combined elements according to the detection result to obtain target elements;
inquiring chemical names corresponding to the target elements, and obtaining the urine sample components of the centrifugal urine sample according to the chemical names.
Optionally, the preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample component comprises the following steps:
inquiring a standard state corresponding to each component in the urine sample components, and extracting fixed components in the urine sample components according to the standard state;
acquiring chemical properties corresponding to the fixed components, and determining dissolved components corresponding to the fixed components according to the chemical properties;
inquiring the consumption proportion of the dissolved components and the fixed components, and counting the quantity value of the fixed components in the centrifugal urine sample;
And preparing a dissolving reagent corresponding to the centrifugal urine sample according to the consumption proportion and the quantity value.
Optionally, the feature extraction of the dissolved urine sample to obtain urine sample features includes:
performing attribute analysis on the components in the dissolved urine sample to obtain a plurality of component attributes;
extracting the characteristics of the plurality of component attributes to obtain a plurality of characteristic attributes;
constructing feature histograms corresponding to the feature attributes, and calculating the complexity coefficient of each histogram in the feature histograms;
and obtaining target characteristic attributes according to the complexity coefficients, and taking the target characteristic attributes as urine sample characteristics of the dissolved urine samples.
Optionally, the calculating the complexity coefficient of each histogram in the feature histogram includes:
the complexity coefficients of each of the feature histograms are calculated by the following formula:
wherein F represents the complexity coefficient of each histogram in the feature histogram, M i Representing the length of a graph line corresponding to an ith histogram in a characteristic histogram, X i Represents the coordinate mean value of the x-axis corresponding to the ith histogram, Y i Represents the coordinate mean value of the y-axis corresponding to the ith histogram, and ∈cd represents the integral of the coordinate mean difference between the x-axis and the y-axis corresponding to the ith histogram.
Optionally, feature extraction is performed on the plurality of component attributes to obtain a plurality of feature attributes, including:
extracting attribute tags corresponding to the plurality of component attributes, and performing vector conversion on each tag in the attribute tags to obtain a plurality of tag vectors;
calculating importance of each of the plurality of tag vectors by the following formula;
wherein Z represents importance of the tag vector, L represents damping coefficient of the tag vector, E i Vector value, E 'representing the ith tag vector' i Representing the vector value of the ith tag vector after dimension reduction, wherein i epsilon T represents the value range of the tag vector;
when the importance is larger than a preset threshold, taking the label vector corresponding to the importance as a feature vector to obtain a plurality of feature vectors;
and obtaining a plurality of characteristic attributes according to the plurality of characteristic vectors.
Optionally, the calculating the dissolution coefficient of the mobile phase includes:
quantitatively sampling the mobile phase to obtain a quantitative sample, and quantitatively configuring the dissolution liquid corresponding to the flow;
mixing the quantitative sample with the dissolution solution to obtain a mixed solution, and detecting the sample content of the quantitative sample in the mixed solution in real time;
Adding the dissolving solution into the mixed solution according to the content of the sample until the content of the sample is zero, and recording the solution dosage of the dissolving solution;
and calculating the solubility of the quantitative sample by combining the quantitative sample and the solution dosage, and taking the solubility as the dissolution coefficient of the mobile phase.
Optionally, said combining said mobile phase, subjecting said dissolved urine sample to chromatographic analysis using said packed chromatographic column to obtain a urine sample chromatogram, comprising:
preparing a detection object corresponding to the filling chromatograph according to the dissolved urine sample;
bonding the detection object with the mobile phase to obtain a target mobile phase;
storing the target mobile phase and the dissolved urine sample in the packed chromatographic column;
utilizing the target mobile phase to react with organic matters in the dissolved urine sample to obtain a plurality of compound organic matters;
the fixed phase is used for respectively fixing the plurality of compound organic matters on the packed chromatographic column;
and carrying out chromatographic analysis on the plurality of organic matters by using the packed chromatographic column to obtain a urine sample chromatogram.
Optionally, the determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram includes:
Identifying the color class of the urine sample chromatogram, and reading the peak value and the peak width corresponding to each color in the color class;
calculating the peak area of each color in the color category according to the peak value and the peak width;
inquiring the organic matter category corresponding to each color, and calculating the association coefficient of the organic matter category and the mass spectrum analyzer;
and sequencing the mass spectrum analyzer according to the relevance and the peak area to obtain a mass spectrum sequence.
In order to solve the above problems, the present invention also provides a detection device for determining sugar content in human urine based on liquid chromatography, the device comprising:
the reagent preparation module is used for obtaining a urine sample to be tested, carrying out physical centrifugation on the urine sample to obtain a centrifugal urine sample, carrying out component detection on the centrifugal urine sample to obtain a urine sample component, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample component;
the chromatographic column preparation module is used for carrying out dissolution treatment on the centrifugal urine sample by utilizing the dissolution reagent to obtain a dissolution urine sample, carrying out characteristic extraction on the dissolution urine sample to obtain urine sample characteristics, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample characteristics, calculating the dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolution urine sample according to the dissolution coefficient;
The chromatographic analysis module is used for filling the chromatographic column with the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram;
the sugar content analysis module is used for scheduling a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by utilizing the mass spectrum analyzer according to the mass spectrum sequence, and analyzing sugar content in the dissolved urine sample according to the sugar-containing substances.
According to the invention, the urine sample to be tested is obtained and subjected to physical centrifugation to obtain a centrifugal urine sample, various soluble substances and colloid substances in the urine sample can be separated, layering phenomenon is formed, and subsequent preparation of a dissolving reagent is facilitated; in addition, the mass spectrum sequence of the mass spectrum analyzer is determined according to the chromatogram of the urine sample by scheduling the mass spectrum analyzer corresponding to the dissolved urine sample, so that the subsequent classification detection analysis of the dissolved urine sample according to the mass spectrum sequence is facilitated. Therefore, the detection method and the device for determining the sugar content in the human urine based on the liquid chromatography provided by the embodiment of the invention can be used for improving the detection efficiency of determining the sugar content in the human urine.
Drawings
FIG. 1 is a flow chart of a detection method for determining sugar content in human urine based on liquid chromatography according to an embodiment of the present invention;
FIG. 2 is a functional block diagram of a detection device for determining sugar content in urine of a person based on liquid chromatography according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of an electronic device for implementing the detection method for determining sugar content in human urine based on liquid chromatography according to an embodiment of the present invention.
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The embodiment of the application provides a detection method for determining sugar content in human urine based on liquid chromatography. In the embodiment of the application, the main execution body of the detection method for determining the sugar content in the urine of the human based on the liquid chromatography includes, but is not limited to, at least one of a server, a terminal and the like, which can be configured to execute the method provided in the embodiment of the application. In other words, the detection method for determining the sugar content in human urine based on liquid chromatography may be performed by software or hardware installed in a terminal device or a server device, and the software may be a blockchain platform. The service end includes but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, and the like. The server may be an independent server, or may be a cloud server that provides cloud services, cloud databases, cloud computing, cloud functions, cloud storage, network services, cloud communications, middleware services, domain name services, security services, content delivery networks (Content Delivery Network, CDN), and basic cloud computing services such as big data and artificial intelligence platforms.
Referring to fig. 1, a flow chart of a detection method for determining sugar content in human urine based on liquid chromatography according to an embodiment of the invention is shown. In this embodiment, the method for detecting sugar content in human urine based on liquid chromatography includes steps S1 to S4:
s1, obtaining a urine sample to be tested, performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, performing component detection on the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample components.
According to the invention, the urine sample to be tested is obtained and subjected to physical centrifugation to obtain the centrifugal urine sample, so that various soluble substances and colloid substances in the urine sample can be separated, and layering phenomenon is formed, thereby facilitating the subsequent preparation of the dissolving reagent.
The urine sample is a sample which needs to be subjected to sugar content detection, the centrifugal urine sample is a sample obtained after the urine sample is subjected to centrifugal treatment, substances in the centrifugal urine sample are separated and are layered, and further, the urine sample can be subjected to physical centrifugation through centrifugal equipment.
The invention can obtain each constituent substance in the centrifugal urine sample by detecting the components of the centrifugal urine sample, thereby facilitating the subsequent configuration of the dissolving reagent of the centrifugal urine sample, wherein the urine sample components are components of the substances contained in the centrifugal urine sample.
As an embodiment of the present invention, the detecting the components of the centrifuged urine sample to obtain urine sample components comprises: sampling the centrifugal urine sample to obtain a urine sample, analyzing the urine sample to obtain analysis elements, mutually combining each element in the analysis elements according to a preset element combination table to form combined elements, inquiring a detection reagent corresponding to the combined elements, detecting the urine sample by using the detection reagent to obtain a detection result, screening the combined elements according to the detection result to obtain target elements, inquiring chemical names corresponding to the target elements, and obtaining urine sample components of the centrifugal urine sample according to the chemical names.
Wherein the urine sampling is obtained after sampling the centrifugal urine sample, the analysis element is all elements contained in the urine sampling, the preset element combination table is a corresponding table of all elements capable of carrying out chemical combination reaction, the detection reagent is a reagent capable of generating obvious visible phenomenon after reacting with the combined element, and the target element is the root of the combined element The chemical name is the name of the target element, such as H 2 O 2 Is named as hydrogen peroxide.
Further, the centrifugal urine sample may be sampled by an associated sampler, the urine sample may be element parsed by an element parser, and the combined elements may be screened by a screening function, including a FILTER function.
According to the invention, the dissolving reagent corresponding to the centrifugal urine sample is prepared according to the urine sample component, so that the dissolving reagent can be used for dissolving insoluble substances in the urine sample component, and convenience is provided for subsequent treatment, wherein the dissolving reagent is a reagent for dissolving substances in some states of non-liquid.
As one embodiment of the present invention, the preparing a dissolving reagent corresponding to the centrifuged urine sample according to the urine sample component comprises: inquiring a standard state corresponding to each component in the urine sample components, extracting fixed components in the urine sample components according to the standard state, obtaining chemical properties corresponding to the fixed components, determining dissolved components corresponding to the fixed components according to the chemical properties, inquiring consumption proportion of the dissolved components to the fixed components, counting quantity values of the fixed components in the centrifugal urine sample, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the consumption proportion and the quantity values.
Wherein the standard state is a state corresponding to each component in the urine sample component under a normal environment, and the standard state comprises a liquid state and a solid state, the fixed component is a component corresponding to the solid state in the urine sample component, the chemical property is a property of the fixed component expressed in chemical change, the dissolved component is a component for converting the solid component into liquid, further, the fixed component in the urine sample component can be extracted by a Soxhlet extractor, the corresponding chemical property can be obtained by a phenomenon expressed by the corresponding chemical reaction of the fixed component, the consumption ratio can be obtained by a chemical reaction equation corresponding to the fixed component, the corresponding dissolved component can be configured by the consumption ratio and the number value, and the dissolved reagent can be obtained after the dissolved component is mixed.
S2, carrying out dissolution treatment on the centrifugal urine sample by utilizing the dissolution reagent to obtain a dissolution urine sample, carrying out feature extraction on the dissolution urine sample to obtain urine sample features, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample features, calculating the dissolution coefficient of the mobile phase, and constructing a chromatographic column of the dissolution urine sample according to the dissolution coefficient.
According to the invention, the dissolving reagent is used for dissolving the centrifugal urine sample to obtain the dissolved urine sample, so that solid matters in the centrifugal urine sample can be dissolved into a liquid state, and further the construction of a subsequent chromatographic column is facilitated, wherein the dissolved urine sample is obtained by dissolving the solid matters in the centrifugal urine sample into the liquid.
According to the invention, the characteristic extraction is carried out on the dissolved urine sample to obtain the urine sample characteristic so as to facilitate the subsequent configuration of the stationary phase and the mobile phase, wherein the urine sample characteristic is the characteristic part corresponding to the dissolved urine sample, such as color, smell and the like.
As one embodiment of the present invention, the feature extraction of the dissolved urine sample to obtain urine sample features includes: performing attribute analysis on components in the dissolved urine sample to obtain a plurality of component attributes, performing feature extraction on the plurality of component attributes to obtain a plurality of feature attributes, constructing feature histograms corresponding to the plurality of feature attributes, calculating complex coefficients of each histogram in the feature histograms, obtaining target feature attributes according to the complex coefficients, and taking the target feature attributes as urine sample features of the dissolved urine sample.
The characteristic histograms are graphical expression forms corresponding to the characteristic attributes, the complexity coefficients represent complexity degrees corresponding to the characteristic histograms, the larger the complexity coefficients are, the more complex the characteristic histograms are, the importance of the characteristics is high, the target characteristic attributes are attributes corresponding to histograms with the largest complexity coefficients, further, attribute analysis can be conducted on components in the dissolved urine sample through an attribute analyzer, the attribute analyzer is compiled by a script language, and feature extraction can be conducted on the plurality of component attributes through a feature extractor.
As an optional embodiment of the present invention, the calculating the complexity coefficient of each of the feature histograms includes:
the complexity coefficients of each of the feature histograms are calculated by the following formula:
wherein F represents the complexity coefficient of each histogram in the feature histogram, M i Representing the length of a graph line corresponding to an ith histogram in a characteristic histogram, X i Represents the coordinate mean value of the x-axis corresponding to the ith histogram, Y i Represents the coordinate mean value of the y-axis corresponding to the ith histogram, and ∈cd represents the integral of the coordinate mean difference between the x-axis and the y-axis corresponding to the ith histogram.
Further, as an optional embodiment of the present invention, performing feature extraction on the plurality of component attributes to obtain a plurality of feature attributes, including: extracting attribute tags corresponding to the plurality of component attributes, carrying out vector conversion on each tag in the attribute tags to obtain a plurality of tag vectors, calculating the importance of each tag vector in the plurality of tag vectors, and taking the tag vector corresponding to the importance as a feature vector to obtain a plurality of feature vectors when the importance is greater than a preset threshold value, and obtaining a plurality of feature attributes according to the plurality of feature vectors.
The attribute labels are corresponding identifiers of the component attributes, the plurality of label vectors are vector expression forms of the attribute labels, the importance degree is the importance degree corresponding to the label vectors, the preset threshold value is 0.8, the attribute labels can also be set according to actual business scenes, the feature vectors are vectors corresponding to the importance degree greater than the preset threshold value, further, the attribute labels corresponding to the plurality of component attributes can be extracted through a label extractor, and vector conversion can be performed on each label in the attribute labels through a Word2vec algorithm.
Further, as an optional embodiment of the present invention, the calculating the importance of each tag vector in the plurality of tag vectors includes:
the importance is calculated by the following formula:
wherein Z represents importance of the tag vector, L represents damping coefficient of the tag vector, E i Vector value, E 'representing the ith tag vector' i The vector value after the dimension reduction of the ith tag vector is represented, and i epsilon T represents the value range of the tag vector.
According to the invention, the stationary phase and the mobile phase of the dissolved urine sample are configured according to the characteristics of the urine sample, and the dissolution coefficient of the mobile phase is calculated, so that the subsequent chromatographic analysis of the dissolved urine sample by the stationary phase and the mobile phase is facilitated, wherein the stationary phase is a carrier for separating and fixing corresponding ions, such as amino bonding, cyano bonding and silica gel, and the mobile phase is used for the subsequent chromatographic analysis of reactive substances, so that the chromatographic reaction is facilitated, and further, the types of the stationary phase and the mobile phase can be determined through the characteristics of the urine sample, and then the configuration is performed in combination with the quantity of the dissolved urine sample.
As an embodiment of the present invention, the calculating the dissolution coefficient of the mobile phase includes: quantitatively sampling the mobile phase to obtain a quantitative sample, quantitatively preparing a solution corresponding to the mobile phase, mixing the quantitative sample with the solution to obtain a mixed solution, detecting the sample content of the quantitative sample in the mixed solution in real time, adding the solution into the mixed solution according to the sample content until the sample content is zero, recording the solution dosage of the solution, calculating the solubility of the quantitative sample by combining the quantitative sample and the solution dosage, and taking the solubility as the dissolution coefficient of the mobile phase.
The quantitative sample is obtained by sampling the mobile phase according to a certain quantity, the dissolution liquid is liquid dissolved by the quantitative sample, the solubility is the mass ratio of the quantitative sample in the dissolution liquid, further, the sample content of the quantitative sample in the mixed solution can be detected in real time through a solute detector, and the solubility can be obtained by calculating the quantity of the quantitative sample and the percentage of the solution dosage.
According to the invention, the chromatographic column for dissolving the urine sample is constructed according to the dissolution coefficient, and the chromatographic analysis can be carried out on the dissolved urine sample through the chromatogram so as to facilitate the subsequent detection of sugar content, wherein the chromatographic column is a column body for carrying out chromatographic analysis, and further, the chromatographic column can be prepared through an organic solvent extraction method.
And S3, filling the chromatographic column by using the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram.
According to the invention, the fixed phase is utilized to fill the chromatographic column, so that the filled chromatographic column is obtained, and the subsequent separation of substances from the dissolved urine sample through the fixed phase is facilitated, wherein the filled chromatographic column is obtained after the fixed phase is filled into the chromatographic column, so that the chromatographic column has a separation function.
According to the invention, by combining the mobile phase, the dissolved urine sample is subjected to chromatographic analysis by using the filled chromatographic column, so that a urine sample chromatogram is obtained, and organic matters contained in the dissolved urine sample can be separated and displayed by the filled chromatographic column, wherein the urine sample chromatogram is an image expression form of organic matters and contents in the dissolved urine sample.
As an embodiment of the present invention, the chromatographic analysis of the dissolved urine sample by the packed chromatographic column in combination with the mobile phase, results in a urine sample chromatogram, comprising: preparing a detection object corresponding to the filling chromatograph according to the dissolved urine sample, bonding the detection object with the mobile phase to obtain a target mobile phase, storing the target mobile phase and the dissolved urine sample in the filling chromatograph, reacting the target mobile phase with organic matters in the dissolved urine sample to obtain a plurality of compound organic matters, fixing the plurality of compound organic matters on the filling chromatograph by the fixed phase, and performing chromatographic analysis on the plurality of organic matters by using the filling chromatograph to obtain a urine sample chromatogram.
The target mobile phase is obtained by bonding the mobile phase with the detection object, the compound organic matter is obtained by reacting the target mobile phase with the organic matter, the detection object and the mobile phase can be bonded through an adsorbent, the target mobile phase and the dissolved urine sample can be stored through a storage in the packed chromatographic column, the plurality of compound organic matters can be respectively fixed on the packed chromatographic column through silica gel pellets on the stationary phase, and chromatographic analysis of the plurality of organic matters can be realized through the matched use of the packed chromatographic column and a chromatographic analysis instrument.
S4, scheduling a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer according to the mass spectrum sequence, and analyzing sugar content in the dissolved urine sample according to the sugar-containing substances.
According to the invention, a mass spectrum sequence of the mass spectrum analyzer is determined according to the chromatogram of the urine sample, so that the subsequent hierarchical detection analysis of the dissolved urine sample according to the mass spectrum sequence is facilitated, wherein the mass spectrum analyzer is a precise and efficient multifunctional analyzer for separating molecules with different molecular weights in a detected object, measuring the molecular weight and analyzing components and structures, and the mass spectrum sequence is an analysis processing sequence corresponding to the mass spectrum analyzer, and further, the mass spectrum analyzer corresponding to the dissolved urine sample can be scheduled through a device manager.
As one embodiment of the present invention, the determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram includes: identifying the color class of the urine sample chromatogram, reading the peak value and the peak width corresponding to each color in the color class, calculating the peak area of each color in the color class according to the peak value and the peak width, inquiring the organic matter class corresponding to each color, calculating the association coefficient of the organic matter class and the mass spectrum analyzer, and sequencing the mass spectrum analyzer according to the association and the peak area to obtain a mass spectrum sequence.
Wherein the peak value is the maximum value of each color in the color class in the urine sample chromatogram, the peak width is the width of each color in the color class in the urine sample chromatogram, the peak area is the area of each color in the urine sample chromatogram, the organic matter class is the class corresponding to the organic matter, and the correlation coefficient is the correlation degree of the organic matter class and the mass spectrometer.
Further, the peak value and peak width corresponding to each color in the color class can be read by a parameter reader, the peak area of each color in the color class can be calculated by an area calculation tool, the area calculation tool is compiled by a script language, and the mass spectrum analyzer can be ranked by a ranking algorithm, wherein the ranking algorithm comprises bubbling ranking, by combining the peak areas and the numerical values of the relevance.
Further, the calculating the correlation coefficient between the organic matter category and the mass spectrometer comprises:
the correlation coefficient is calculated by the following formula:
wherein G represents the correlation coefficient between the organic matter type and the mass spectrometer, N represents the total number of the organic matter type and the mass spectrometer, and H l Characteristic value, log H, representing the first category of organic matter categories l A logarithmic value K corresponding to the characteristic value of the first category in the organic matter category l Representing the attribute value, log K, of the first analyzer in a mass spectrometer l Represents a logarithmic value corresponding to an attribute value of a first analyzer in the mass spectrometer, maxmax () represents a maximum value of the logarithmic value difference, minmin () represents a minimum value of the logarithmic value difference, and ρ represents a conversion coefficient corresponding to the characteristic value and the attribute value.
The present invention can improve the accuracy of detecting a sugar-containing substance in a dissolved urine sample by detecting the sugar-containing substance in the dissolved urine sample by using the mass spectrometer and analyzing the sugar content in the dissolved urine sample based on the sugar-containing substance, wherein the sugar-containing substance is a substance containing sugar, and the sugar content in the dissolved urine sample can be obtained by analyzing the percentage of sugar in the sugar-containing substance and further comparing the carbohydrate by detecting the carbohydrate in the dissolved urine sample by using the mass spectrometer.
According to the invention, the urine sample to be tested is obtained and subjected to physical centrifugation to obtain a centrifugal urine sample, various soluble substances and colloid substances in the urine sample can be separated, layering phenomenon is formed, and subsequent preparation of a dissolving reagent is facilitated; in addition, the mass spectrum sequence of the mass spectrum analyzer is determined according to the chromatogram of the urine sample by scheduling the mass spectrum analyzer corresponding to the dissolved urine sample, so that the subsequent classification detection analysis of the dissolved urine sample according to the mass spectrum sequence is facilitated. Therefore, the detection method for determining the sugar content in the human urine based on the liquid chromatography provided by the embodiment of the invention can be used for improving the detection efficiency of determining the sugar content in the human urine.
Fig. 2 is a functional block diagram of a detection device for determining sugar content in urine of a person based on liquid chromatography according to an embodiment of the present invention.
The detection device 100 for measuring the sugar content in human urine based on liquid chromatography according to the present invention may be mounted in an electronic apparatus. Depending on the functions implemented, the detection device 100 for determining the sugar content in human urine based on liquid chromatography may include a reagent preparation module 101, a chromatographic column preparation module 102, a chromatographic analysis module 103, and a sugar content analysis module 104. The module of the invention, which may also be referred to as a unit, refers to a series of computer program segments, which are stored in the memory of the electronic device, capable of being executed by the processor of the electronic device and of performing a fixed function.
In the present embodiment, the functions concerning the respective modules/units are as follows:
the reagent preparation module 101 is configured to obtain a urine sample to be tested, perform physical centrifugation on the urine sample to obtain a centrifugal urine sample, perform component detection on the centrifugal urine sample to obtain a urine sample component, and prepare a dissolution reagent corresponding to the centrifugal urine sample according to the urine sample component;
the chromatographic column preparation module 102 is configured to perform dissolution treatment on the centrifugal urine sample by using the dissolution reagent to obtain a dissolved urine sample, perform feature extraction on the dissolved urine sample to obtain urine sample features, configure a stationary phase and a mobile phase of the dissolved urine sample according to the urine sample features, calculate a dissolution coefficient of the mobile phase, and prepare a chromatographic column of the dissolved urine sample according to the dissolution coefficient;
The chromatographic analysis module 103 is configured to perform filling with respect to the chromatographic column by using the fixed phase, obtain a filled chromatographic column, and perform chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase, to obtain a urine sample chromatogram;
the sugar content analysis module 104 is configured to schedule a mass spectrometer corresponding to the dissolved urine sample, determine a mass spectrum sequence of the mass spectrometer according to the urine sample chromatogram, detect sugar-containing substances in the dissolved urine sample according to the mass spectrum sequence by using the mass spectrometer, and analyze sugar content in the dissolved urine sample according to the sugar-containing substances.
In detail, each module in the detection device 100 for determining the sugar content in human urine based on liquid chromatography in the embodiment of the present application adopts the same technical means as the detection method for determining the sugar content in human urine based on liquid chromatography described in fig. 1, and can produce the same technical effects, which are not described herein.
Fig. 3 is a schematic structural diagram of an electronic device 1 for implementing a method for detecting sugar content in urine of a person based on liquid chromatography according to an embodiment of the present invention.
The electronic device 1 may comprise a processor 10, a memory 11, a communication bus 12 and a communication interface 13, and may further comprise a computer program stored in the memory 11 and executable on the processor 10, such as a detection method program for determining the sugar content in human urine based on liquid chromatography.
The processor 10 may be formed by an integrated circuit in some embodiments, for example, a single packaged integrated circuit, or may be formed by a plurality of integrated circuits packaged with the same function or different functions, including one or more central processing units (Central Processing Unit, CPU), a microprocessor, a digital processing chip, a graphics processor, a combination of various control chips, and so on. The processor 10 is a Control Unit (Control Unit) of the electronic device 1, connects the respective components of the entire electronic device using various interfaces and lines, executes or executes programs or modules stored in the memory 11 (for example, executes a detection method program for measuring the sugar content in human urine based on liquid chromatography, etc.), and invokes data stored in the memory 11 to perform various functions of the electronic device and process data.
The memory 11 includes at least one type of readable storage medium including flash memory, a removable hard disk, a multimedia card, a card type memory (e.g., SD or DX memory, etc.), a magnetic memory, a magnetic disk, an optical disk, etc. The memory 11 may in some embodiments be an internal storage unit of the electronic device, such as a mobile hard disk of the electronic device. The memory 11 may in other embodiments also be an external storage device of the electronic device, such as a plug-in mobile hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card) or the like, which are provided on the electronic device. Further, the memory 11 may also include both an internal storage unit and an external storage device of the electronic device. The memory 11 may be used not only for storing application software installed in an electronic device and various types of data, such as a code of a detection method program for measuring the sugar content in human urine based on liquid chromatography, but also for temporarily storing data that has been output or is to be output.
The communication bus 12 may be a peripheral component interconnect standard (Peripheral Component Interconnect, PCI) bus, or an extended industry standard architecture (Extended Industry Standard Architecture, EISA) bus, among others. The bus may be classified as an address bus, a data bus, a control bus, etc. The bus is arranged to enable a connection communication between the memory 11 and at least one processor 10 etc.
The communication interface 13 is used for communication between the electronic device 1 and other devices, including a network interface and a user interface. Optionally, the network interface may include a wired interface and/or a wireless interface (e.g., WI-FI interface, bluetooth interface, etc.), typically used to establish a communication connection between the electronic device and other electronic devices. The user interface may be a Display (Display), an input unit such as a Keyboard (Keyboard), or alternatively a standard wired interface, a wireless interface. Alternatively, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, an OLED (Organic Light-Emitting Diode) touch, or the like. The display may also be referred to as a display screen or display unit, as appropriate, for displaying information processed in the electronic device and for displaying a visual user interface.
Fig. 3 shows only an electronic device with components, it being understood by a person skilled in the art that the structure shown in fig. 3 does not constitute a limitation of the electronic device 1, and may comprise fewer or more components than shown, or may combine certain components, or may be arranged in different components.
For example, although not shown, the electronic device 1 may further include a power source (such as a battery) for supplying power to each component, and preferably, the power source may be logically connected to the at least one processor 10 through a power management device, so that functions of charge management, discharge management, power consumption management, and the like are implemented through the power management device. The power supply may also include one or more of any of a direct current or alternating current power supply, recharging device, power failure detection circuit, power converter or inverter, power status indicator, etc. The electronic device 1 may further include various sensors, bluetooth modules, wi-Fi modules, etc., which will not be described herein.
It should be understood that the embodiments described are for illustrative purposes only and are not limited to this configuration in the scope of the patent application.
The detection method program stored in the memory 11 of the electronic device 1 for determining the sugar content in human urine based on liquid chromatography is a combination of a plurality of instructions, and when running in the processor 10, it can be implemented that:
obtaining a urine sample to be tested, performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, performing component detection on the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample components;
Performing dissolution treatment on the centrifugal urine sample by using the dissolution reagent to obtain a dissolution urine sample, performing feature extraction on the dissolution urine sample to obtain urine sample features, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample features, calculating a dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolution urine sample according to the dissolution coefficient;
filling the chromatographic column with the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram;
and dispatching a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer according to the mass spectrum sequence, and analyzing the sugar content in the dissolved urine sample according to the sugar-containing substances.
In particular, the specific implementation method of the above instructions by the processor 10 may refer to the description of the relevant steps in the corresponding embodiment of the drawings, which is not repeated herein.
Further, the modules/units integrated in the electronic device 1 may be stored in a computer readable storage medium if implemented in the form of software functional units and sold or used as separate products. The computer readable storage medium may be volatile or nonvolatile. For example, the computer readable medium may include: any entity or device capable of carrying the computer program code, a recording medium, a U disk, a removable hard disk, a magnetic disk, an optical disk, a computer Memory, a Read-Only Memory (ROM).
The present invention also provides a computer readable storage medium storing a computer program which, when executed by a processor of an electronic device, can implement:
obtaining a urine sample to be tested, performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, performing component detection on the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample components;
performing dissolution treatment on the centrifugal urine sample by using the dissolution reagent to obtain a dissolution urine sample, performing feature extraction on the dissolution urine sample to obtain urine sample features, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample features, calculating a dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolution urine sample according to the dissolution coefficient;
filling the chromatographic column with the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram;
and dispatching a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer according to the mass spectrum sequence, and analyzing the sugar content in the dissolved urine sample according to the sugar-containing substances.
In the several embodiments provided in the present invention, it should be understood that the disclosed apparatus, device and method may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the modules is merely a logical function division, and there may be other manners of division when actually implemented.
The modules described as separate components may or may not be physically separate, and components shown as modules may or may not be physical units, may be located in one place, or may be distributed over multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional module in the embodiments of the present invention may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units can be realized in a form of hardware or a form of hardware and a form of software functional modules.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof.
The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims shall not be construed as limiting the claim concerned.
The embodiment of the application can acquire and process the related data based on the artificial intelligence technology. Among these, artificial intelligence (Artificial Intelligence, AI) is the theory, method, technique and application system that uses a digital computer or a digital computer-controlled machine to simulate, extend and extend human intelligence, sense the environment, acquire knowledge and use knowledge to obtain optimal results.
Furthermore, it is evident that the word "comprising" does not exclude other elements or steps, and that the singular does not exclude a plurality. A plurality of units or means recited in the system claims can also be implemented by means of software or hardware by means of one unit or means. The terms first, second, etc. are used to denote a name, but not any particular order.
Finally, it should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (6)
1. A detection method for determining sugar content in human urine based on liquid chromatography, the method comprising:
obtaining a urine sample to be tested, performing physical centrifugation on the urine sample to obtain a centrifugal urine sample, performing component detection on the centrifugal urine sample to obtain urine sample components, and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the urine sample components;
performing dissolution treatment on the centrifugal urine sample by using the dissolution reagent to obtain a dissolution urine sample, performing feature extraction on the dissolution urine sample to obtain urine sample features, configuring a stationary phase and a mobile phase of the dissolution urine sample according to the urine sample features, calculating a dissolution coefficient of the mobile phase, and preparing a chromatographic column of the dissolution urine sample according to the dissolution coefficient;
the feature extraction of the dissolved urine sample to obtain urine sample features comprises the following steps:
performing attribute analysis on the components in the dissolved urine sample to obtain a plurality of component attributes;
extracting the characteristics of the plurality of component attributes to obtain a plurality of characteristic attributes;
constructing feature histograms corresponding to the feature attributes, and calculating the complexity coefficient of each histogram in the feature histograms;
Obtaining target characteristic attributes according to the complexity coefficients, and taking the target characteristic attributes as urine sample characteristics of the dissolved urine samples;
the calculating the complexity coefficients of each of the feature histograms includes:
the complexity coefficients of each of the feature histograms are calculated by the following formula:
wherein F represents the complexity coefficient of each histogram in the feature histogram, M i Representing the length of a graph line corresponding to an ith histogram in a characteristic histogram, X i Represents the coordinate mean value of the x-axis corresponding to the ith histogram, Y i Representing the coordinate mean value of the y-axis corresponding to the ith histogram, and (+_cd) representing the integral of the coordinate mean difference between the x-axis and the y-axis corresponding to the ith histogram;
filling the chromatographic column with the fixed phase to obtain a filled chromatographic column, and carrying out chromatographic analysis on the dissolved urine sample by using the filled chromatographic column in combination with the mobile phase to obtain a urine sample chromatogram;
and dispatching a mass spectrum analyzer corresponding to the dissolved urine sample, determining a mass spectrum sequence of the mass spectrum analyzer according to the urine sample chromatogram, detecting sugar-containing substances in the dissolved urine sample by using the mass spectrum analyzer according to the mass spectrum sequence, and analyzing the sugar content in the dissolved urine sample according to the sugar-containing substances.
2. The method for detecting sugar content in human urine based on liquid chromatography according to claim 1, wherein said detecting the components of the centrifuged urine sample to obtain urine sample components comprises:
sampling the centrifugal urine sample to obtain a urine sample, and carrying out element analysis on the urine sample to obtain analysis elements;
combining each element in the analysis elements according to a preset element combination table to form combined elements;
inquiring a detection reagent corresponding to the combined element, and detecting the urine sample by using the detection reagent to obtain a detection result;
screening the combined elements according to the detection result to obtain target elements;
inquiring chemical names corresponding to the target elements, and obtaining the urine sample components of the centrifugal urine sample according to the chemical names.
3. The method for detecting sugar content in human urine based on liquid chromatography according to claim 1, wherein said preparing a dissolving reagent corresponding to said centrifuged urine sample based on said urine sample component comprises:
inquiring a standard state corresponding to each component in the urine sample components, and extracting fixed components in the urine sample components according to the standard state;
Acquiring chemical properties corresponding to the fixed components, and determining dissolved components corresponding to the fixed components according to the chemical properties;
inquiring the consumption proportion of the dissolved components and the fixed components, and counting the quantity value of the fixed components in the centrifugal urine sample;
and preparing a dissolving reagent corresponding to the centrifugal urine sample according to the consumption proportion and the quantity value.
4. The method for detecting the sugar content in the urine of the human based on the liquid chromatography according to claim 1, wherein the feature extraction is performed on the plurality of component attributes to obtain a plurality of feature attributes, comprising:
extracting attribute tags corresponding to the plurality of component attributes, and performing vector conversion on each tag in the attribute tags to obtain a plurality of tag vectors;
calculating importance of each of the plurality of tag vectors by the following formula;
wherein Z represents importance of the tag vector, L represents damping coefficient of the tag vector, E i Vector value representing the ith tag vector, E ’ i Representing the vector value of the ith tag vector after dimension reduction, wherein i epsilon T represents the value range of the tag vector;
when the importance is larger than a preset threshold, taking the label vector corresponding to the importance as a feature vector to obtain a plurality of feature vectors;
And obtaining a plurality of characteristic attributes according to the plurality of characteristic vectors.
5. The method for detecting sugar content in human urine based on liquid chromatography according to claim 1, wherein said calculating the dissolution coefficient of the mobile phase comprises:
quantitatively sampling the mobile phase to obtain a quantitative sample, and quantitatively configuring the dissolution liquid corresponding to the flow;
mixing the quantitative sample with the dissolution solution to obtain a mixed solution, and detecting the sample content of the quantitative sample in the mixed solution in real time;
adding the dissolving solution into the mixed solution according to the content of the sample until the content of the sample is zero, and recording the solution dosage of the dissolving solution;
and calculating the solubility of the quantitative sample by combining the quantitative sample and the solution dosage, and taking the solubility as the dissolution coefficient of the mobile phase.
6. The method for detecting sugar content in human urine based on liquid chromatography according to claim 1, wherein said combining the mobile phase and subjecting the dissolved urine sample to chromatographic analysis using the packed chromatographic column to obtain a urine sample chromatogram comprises:
preparing a detection object corresponding to the filling chromatograph according to the dissolved urine sample;
Bonding the detection object with the mobile phase to obtain a target mobile phase;
storing the target mobile phase and the dissolved urine sample in the packed chromatographic column;
utilizing the target mobile phase to react with organic matters in the dissolved urine sample to obtain a plurality of compound organic matters;
the fixed phase is used for respectively fixing the plurality of compound organic matters on the packed chromatographic column;
and carrying out chromatographic analysis on the plurality of organic matters by using the packed chromatographic column to obtain a urine sample chromatogram.
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| JP2001013127A (en) * | 1999-06-28 | 2001-01-19 | Hitachi Ltd | Liquid chromatography-mass spectrometer |
| CN110333308A (en) * | 2019-07-18 | 2019-10-15 | 四川大学 | While a kind of sensitivity, accuracy are high in measurement urine NNAL and cotinine method |
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| WO2011025602A1 (en) * | 2009-07-20 | 2011-03-03 | The Board Of Regents Of The University Of Texas System | Combinational multidomain mesoporous chips and a method for fractionation, stabilization, and storage of biomolecules |
| US9339813B2 (en) * | 2009-12-18 | 2016-05-17 | Koninklijke Philips N.V. | Substance determining apparatus |
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| JP2001013127A (en) * | 1999-06-28 | 2001-01-19 | Hitachi Ltd | Liquid chromatography-mass spectrometer |
| CN110333308A (en) * | 2019-07-18 | 2019-10-15 | 四川大学 | While a kind of sensitivity, accuracy are high in measurement urine NNAL and cotinine method |
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| 超高效液相色谱-串联质谱法监测血和尿中吗啡-３-葡萄糖醛酸苷;冷晓晓等;理化检验-化学分册;第54卷(第11期);第1.3.2节、第2.1-2.3节 * |
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