CN115851440A

CN115851440A - Superposable constant-temperature constant-humidity three-gas oscillation cell culture device

Info

Publication number: CN115851440A
Application number: CN202211680235.0A
Authority: CN
Inventors: 宋波; 陈丽娟; 周洋
Original assignee: Suzhou Huanmei Biomedical Technology Co ltd
Current assignee: Suzhou Huanmei Biomedical Technology Co ltd
Priority date: 2022-12-27
Filing date: 2022-12-27
Publication date: 2023-03-28
Anticipated expiration: 2042-12-27
Also published as: CN115851440B

Abstract

The invention discloses a superposable constant-temperature constant-humidity three-gas oscillation cell culture device, which comprises a box body, wherein an inner cavity is formed in the box body; the inner bottom wall of the inner cavity is provided with an oscillation driving device, the top of the oscillation driving device is provided with a clamp locking plate, and the clamp locking plate is provided with a quick locking device and a guide rail; the back in inner chamber sets up the wind channel, just the inside in wind channel is equipped with refrigerating plant, heating device, air current in proper order and leads and mixes device and fan from bottom to top to the wind channel lower part is equipped with temperature detection sensor, temperature recording sensor, carbon dioxide sensor, oxygen sensor and humidity transducer, humidifying device and dehydrating unit are equipped with to wind channel right side downside, specifically opens fast and the function of locking jig jam plate, does not have the screw thread and screws up or not screw up the position and the easy not hard up problem, and the reliability is better, has simultaneously that humidity control is accurate, gas mixing is even and the chamber door transparent window atomization does not appear, satisfies humidity control within 1.5%.

Description

Superposable constant-temperature constant-humidity three-gas oscillation cell culture device

Technical Field

The invention relates to the technical field of biomedical equipment for cell culture, in particular to a cell culture device capable of superposing constant temperature and humidity three-gas oscillation.

Background

The three-gas oscillation cell culture device is a biomedical device for cell culture. Its advantages are precise temp and CO ₂ Measurement and control system and precise O ₂ The system comprises a measurement and control system, a precise RH humidity measurement and control system and a humanized operation design, can most closely simulate the growth environment in vivo, is provided with a microcomputer control system, can automatically start mechanisms such as a heating system and a gas homogenizing system, and can visually display and operate. And because the device has the functions of constant temperature and humidity, three-gas control, an oscillation mechanism and the like, the device can completely simulate the working environment of animal cells in vivo, so the device is widely popularized and used in the biomedical field and the related field of laboratories, and is particularly suitable for cell culture under the conditions of low oxygen, high oxygen and normal oxygen in vitro. However, the current cell culture device still has the following defects:

firstly, the locking structure in the existing cell culture device is generally in a threaded locking mode, so that not only is quick locking operation inconvenient, but also the problem that the threads are not screwed tightly or not screwed in place and are easy to loosen exists, the reliability is poor, and potential safety hazards exist; and also wastes time for scientific research personnel.

Secondly, when the existing cell culture device is used, water vapor is easily accumulated on a cabinet door glass window and condensed and gathered into water flow to slide off, so that scientific research personnel can not observe the internal situation from the outside of the device conveniently; some of the water condensation preventing structures are complex, low in reliability and high in cost.

Thirdly, although the existing cell culture device can maintain the temperature inside the device by refrigerating or heating through a constant temperature mechanism, the cold and hot air is easy to be layered in the box, the problems of cold air sinking and hot air floating are caused, the problem of uneven temperature distribution inside the cavity is caused, and the requirements of high temperature precision and high uniformity are not met;

fourth, current cell culture device carries environment steam or culture medium moisture evaporation in the blake bottle easily when the air gets into the cavity to influence the inside humidity condition of culture apparatus, can not satisfy the humidity requirement or the PH requirement of specific cell, be unfavorable for the constant humidity of cell to cultivate or the cultivation requirement of invariable PH.

Therefore, there is an urgent need to improve the disadvantages, and the present invention provides a cell culture apparatus capable of superposing three gas oscillations at constant temperature and humidity by improving the existing structure and disadvantages.

Disclosure of Invention

The invention aims to provide a cell culture device capable of superposing constant temperature and humidity three-gas oscillation, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a cell culture device capable of superposing three gases at constant temperature and humidity comprises a box body, a box cover and a cell body, wherein an inner cavity is arranged in the box body;

the inner bottom wall of the inner cavity is provided with an oscillation driving device, the top of the oscillation driving device is provided with a clamp locking plate, the clamp locking plate is provided with a quick locking device and a guide rail, the quick locking device comprises a base, a lower seat, an upper seat, a pin shaft, a return pressure spring, a pressing block, a first pin, a U-shaped connecting rod, a handle seat, a magnet, a control handle, a third pin and a second pin, the base is fixed on a support of the oscillation driving device, the lower seat is embedded with the lower seat, the lower seat is provided with a first notch, a horizontal shaft is arranged in the first notch, the horizontal shaft is rotatably connected with the pin shaft, the lower seat is connected with the upper seat through the pin shaft, the pin shaft penetrates through the second notch, the upper seat is fixed on the clamp locking plate, the return pressure spring is sleeved outside the pin shaft, the top end of the return pressure spring is connected with the pressing block, the pressing block is rotatably connected with the U-shaped connecting rod through the first pin and is rotatably connected with the U-shaped connecting rod through the second pin, the handle seat is rotatably connected with the handle seat through the third pin shaft, and is rotatably connected with the control handle seat;

the back in inner chamber sets up the wind channel, just the inside in wind channel is equipped with refrigerating plant, heating device, wind flow and leads and mixes device and fan from bottom to top in proper order to the wind channel lower part is equipped with temperature detection sensor, temperature recording sensor, carbon dioxide sensor, oxygen sensor and humidity transducer, wind channel right side downside is equipped with humidification device and dehydrating unit.

Preferably, the front surface of the box body is provided with a box door through a hinge structure, a heat resistance strip is arranged between the inner cavity and the box body, and the inner cavity is contacted with the box body through the heat resistance strip;

the bottom of box is equipped with stack device, and stack device is including stack swash plate, oblique screw hole roof, screwed pipe, U-shaped board, connecting piece, the panel beating of falling U-shaped and screw to the back of box is equipped with drainage device, and the right side of box is equipped with humidification cask hanging flower basket device moreover.

Preferably, the chamber door is including door shell panel beating and visual plastic slab, and the outside of door shell panel beating inlays and is equipped with visual plastic slab to visual plastic slab divide into prevents perspective region and transparent region, prevents moreover that the perspective region is regional through the matte that carries out milling process or chemical corrosion formation to the all peripheral faces of visual plastic slab, and transparent region is the protruding region of visual plastic slab simultaneously.

Preferably, the chamber door is still including annular heating rubber strip, annular drier collecting chamber, transparent plastic board and heat preservation, the protruding region of visual plastic board with be provided with annular heating rubber strip between the transparent plastic board the inner circle of annular heating rubber strip is provided with annular drier collecting chamber, annular drier collecting chamber has a plurality of holes, visual plastic board prevent the perspective regional with set up the heat preservation between the door shell panel beating.

Preferably, the wind current guiding mixes the device including left guide plate, right guide plate, left flow distribution plate and right flow distribution plate, and left guide plate and right guide plate symmetry set up in the inside that the wind current guided mixes the device to left flow distribution plate and right flow distribution plate symmetry set up in the inside that the wind current guided mixes the device, and left guide plate and right flow distribution plate are located the below of left flow distribution plate and right flow distribution plate respectively moreover.

Preferably, the dehumidification device comprises an air suction pump, a first water vapor filter, a first electromagnetic valve, a second electromagnetic valve and a heater, wherein one end of the air suction pump is connected with the first water vapor filter, the other end of the air suction pump is connected with the first electromagnetic valve, one end of the first electromagnetic valve is connected with a pipeline from an inner cavity, and meanwhile, the heater is arranged on a branch pipeline between the first water vapor filter and the second electromagnetic valve;

the dehumidification device is characterized by further comprising a moisture exhaust air pump, a temperature sensor, a moisture exhaust electromagnetic valve and a water vapor filter II, the heater and the branch pipeline are arranged and wound with the temperature sensor, one end of the heater is connected with the moisture exhaust air pump, the moisture exhaust electromagnetic valve is arranged on the branch pipeline between the suction pump and the water vapor filter I, and one end of the moisture exhaust air pump is connected with the water vapor filter II.

Preferably, the bottom of stack swash plate is connected with oblique screw hole roof, and the top welding of stack swash plate has the screwed pipe to the outside welding of screwed pipe has the U-shaped board, and the avris fixedly connected with connecting piece of U-shaped board moreover, and U-shaped board and connecting piece are fixed in the shape of falling U panel beating simultaneously.

Preferably, drainage device is including water drainage mouth, hose, pipe and U-shaped frid, and the outer wall through connection of inner chamber has water drainage mouth to water drainage mouth's tip fixedly connected with hose, the inboard at the pipe is fixed to the hose block moreover, and the pipe links to each other with U-shaped frid welding simultaneously.

Preferably, the drainage device further comprises a water outlet hard pipe and a one-way valve, the end part of the hose is fixedly connected with the water outlet hard pipe, and the one-way valve is arranged between the hose and the water outlet hard pipe.

Preferably, the humidifying bucket hanging basket device comprises a porous hanging basket, a wave hanging frame and a reinforcing plate, the porous hanging basket is fixedly connected with the wave hanging frame through bolts, and the reinforcing plate is welded at the back of the wave hanging frame.

The invention provides a cell culture device capable of superposing three gas oscillations at constant temperature and humidity, which has the following beneficial effects: the utility model provides a three gas of constant temperature and humidity that can superpose shakes and swings cell culture device, inner circle through at annular heating rubber strip is provided with annular drier collecting chamber, the inside heating resistor silk that has of annular heating rubber strip, play the heating effect, make the protruding region of visual plastic slab and the gas between the transparent plastic slab obtain the heating, produce the heating to the transparent region, the visual plastic slab that prevents the chamber door produces water smoke, and be provided with quick locking device, can lock fast and target in place, practice thrift scientific research personnel's valuable time, there is not the problem that the screw thread is twisted untight and easily becomes flexible, additionally, set up the distinguished and admirable mixing device in the wind channel, through left guide plate, right side guide plate, the cooperation of left side flow distribution plate and right flow distribution plate is used and is reached the purpose of homogeneous mixing cold and hot air, in addition, still be equipped with the dehydrating unit, can dehumidify the processing to the air that gets into the cavity, carry steam in the air of avoiding getting into the box, influence the inside humidity constant control of device.

(1) The quick locking device is arranged, and the base, the lower seat, the upper seat, the pin shaft, the return pressure spring, the pressing block, the pin I, the U-shaped link rod, the handle seat, the magnet, the control handle, the pin III, the pin II, the first notch and the second notch are matched for use, so that when the handle is pressed downwards, quick locking is realized, the handle seat is further fixed through the suction force of the magnet after locking, and after the handle is lifted, the handle and the quick mounting device are rotated by 90 degrees along the first notch and the second notch, so that the quick mounting device is separated from the upper seat, and the clamp locking plate is quickly pulled out; through the actions, the quick locking and the quick unlocking are realized, the precious time of scientific research personnel is saved, compared with a thread locking mode, the problem that the threads are not screwed tightly or are not screwed to the proper position and are easy to loosen is solved, and the reliability is better;

(2) The door is provided with the door, the door comprises a door shell metal plate, a visible plastic plate, an annular heating rubber strip, an annular drying agent storage bin, a transparent plastic plate and a heat insulation layer, and a fog surface area (anti-transparent area) is formed by milling or chemically corroding the peripheral surface of the visible plastic plate, so that the fog surface area shields the internal structure of the door, and the door has attractive appearance and confidentiality; the raised area of the visible plastic plate is a transparent area, and the annular drying agent storage bin with drying agent placed inside is arranged at the inner ring of the annular heating rubber strip, so that the annular heating rubber strip plays a role in heating, gas between the raised area of the visible plastic plate and the transparent plastic plate is heated, the transparent area is heated, water mist in the transparent area of the visible plastic plate is prevented from being generated, and the anti-condensation water structure of the box door is simple, good in effect and low in cost;

(3) The invention is provided with the airflow guiding and mixing device, the cold air and the hot air form vortex mixing in the middle after passing through the left flow guide plate and the right flow guide plate, and the cold air and the hot air can be further mixed through the left flow distribution plate and the right flow distribution plate, so that the temperature entering the cavity is more uniform, and the layering of cold air is prevented.

(4) The dehumidifying device is arranged, the air flow direction is controlled by the first electromagnetic valve and the second electromagnetic valve, and water vapor is filtered by the first water vapor filter of the water vapor filter, so that dry air enters the cavity and the humidity of the air in the cavity is reduced; then, water in the first water vapor filter is taken away through a moisture exhaust pump, a second water vapor filter and a moisture exhaust electromagnetic valve so as to keep the first water vapor filter in a dry state; when regulating and controlling the inner chamber humidity, can not additionally get into new steam and other impurity to realize the inside humidity of inner chamber and constantly control, and simple structure, pollution-free need not to maintain the maintenance and change or clean the device, low cost, dehumidification is efficient, and the dependable performance satisfies that humidity control is within 1.5%, and humidity control range reaches 20% -95%.

Drawings

FIG. 1 is a schematic view of an external structure of a cell culture apparatus capable of superposing three gas oscillations at constant temperature and humidity according to the present invention;

FIG. 2 is a schematic view showing the structural connection of the heat-resistant strip and the inner chamber of a cell culture apparatus capable of superposing three gas oscillations at constant temperature and humidity according to the present invention;

FIG. 3 is a schematic rear view of a fan of a cell culture apparatus according to the present invention;

FIG. 4 is a schematic diagram of a side view of a blower of a cell culture apparatus capable of superposing constant temperature and humidity three-gas oscillation according to the present invention;

FIG. 5 is a schematic structural diagram of a metal plate cross-sectional view of a door shell of a superposable constant-temperature constant-humidity three-gas oscillation cell culture device according to the present invention;

FIG. 6 is a schematic view of a three-dimensional structure of a rapid locking device-guide rail of a superposable constant temperature and humidity three-gas oscillation cell culture device of the present invention;

FIG. 7 is a schematic view showing the connection of a supporting base-pin structure of a cell culture apparatus according to the present invention;

FIG. 8 is a schematic view showing the structural connection between a handle seat and a control handle of a superposable constant temperature and humidity three-gas oscillation cell culture device according to the present invention;

FIG. 9 is a schematic diagram showing the structural connection of a left baffle-a right baffle of a superposable constant temperature and humidity three-gas oscillation cell culture device according to the present invention;

FIG. 10 is a schematic view showing the structural connection of the intracavity-getter pump of the superposable constant-temperature constant-humidity three-gas oscillation cell culture device according to the present invention;

FIG. 11 is a schematic connection diagram of a screwed pipe-inverted U-shaped metal plate structure of a cell culture device capable of superposing constant temperature and humidity three-gas oscillation according to the present invention;

FIG. 12 is a schematic diagram showing the structural connection of a U-shaped plate-connecting member of a cell culture apparatus capable of superposing three gas oscillations at constant temperature and humidity according to the present invention;

FIG. 13 is a schematic cross-sectional view of a circular tube of a cell culture apparatus with a superimposed constant temperature and humidity three-gas oscillation according to the present invention;

FIG. 14 is a schematic view of a multi-hole hanging basket-wave rack structure of a cell culture apparatus capable of superposing constant temperature and humidity three-gas oscillation.

In the figure: 1. a box body; 101. a first case; 102. a first case; 2. a box door; 201. a door shell sheet metal; 202. a visible plastic panel; 203. annularly heating the rubber strip; 204. an annular desiccant storage bin; 205. a transparent plastic plate; 206. a heat-insulating layer; 3. an inner cavity; 4. heat-resistant strips; 5. an oscillation drive device; 6. locking the plate by the clamp; 7. a quick locking device; 701. a base; 702. a lower seat; 7021. a first notch; 7021. a horizontal axis; 703. an upper seat; 7031. a second notch; 704. a pin shaft; 705. a return pressure spring; 706. briquetting; 707. a first pin; 708. a U-shaped link; 709. a handle seat; 710. a magnet; 711. a control handle; 712. a third pin; 713. a second pin; 8. a guide rail; 9. a refrigeration device; 10. a heating device; 11. an air flow guiding and mixing device; 1101. a left baffle; 1102. a right baffle; 1103. a left flow distribution plate; 1104. a right flow distribution plate; 12. a fan; 13. a temperature detection sensor; 14. a temperature recording sensor; 15. a carbon dioxide sensor; 16. an oxygen sensor; 17. a humidity sensor; 18. a humidifying device; 19. a dehumidifying device; 1901. a getter pump; 1902. a first water vapor filter; 1903. a first electromagnetic valve; 1904. a second electromagnetic valve; 1905. a moisture exhausting air pump; 1906. a heater; 1907. a temperature sensor; 1908. a moisture removal solenoid valve; 1909. a second water vapor filter; 20. a superimposing device; 2001. superposing inclined plates; 2002. a top plate with an oblique threaded hole; 2003. a threaded pipe; 2004. a U-shaped plate; 2005. a connecting member; 2006. an inverted U-shaped metal plate; 2007. a screw; 21. a drainage device; 2101. a water discharge nozzle; 2102. a hose; 2103. a circular tube; 2104. a U-shaped groove plate; 2105. a water outlet hard pipe; 2106. a one-way valve; 22. an air duct; 23. a humidifying bucket basket device; 2301. a porous basket; 2302. a wave hanger; 2303. a reinforcing plate.

Detailed Description

The present invention is described in detail with reference to the embodiments shown in the drawings, but it should be understood that these embodiments are not intended to limit the present invention, and those skilled in the art should understand that functional, methodological, or structural equivalents or substitutions made by these embodiments are within the scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, merely for convenience of description and simplification of the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention.

For convenience of explanation, the present embodiment will be described by taking a structure in which two cases 1 are stacked together as an example, and specifically referring to fig. 1, an upper case is a first case 101, and a lower case is a second case 102.

As shown in fig. 1, fig. 2, fig. 6, fig. 7 and fig. 8, a cell culture apparatus capable of superposing three gases at constant temperature and humidity comprises a box body 1 and an air duct 22, wherein a box door 2 is movably installed on the front surface of the box body 1 through a hinge, an inner cavity 3 is arranged inside the box body 1, a heat-blocking strip 4 is arranged between the inner cavity 3 and the box body 1, the inner cavity 3 is contacted with the box body 1 through the heat-blocking strip 4, an oscillation driving device 5 is installed on the inner bottom wall of the inner cavity 3, a clamp locking plate 6 is arranged on the top of the oscillation driving device 5, the clamp locking plate 6 is provided with a quick locking device 7 and a guide rail 8, the quick locking device 7 comprises a base 701, a lower seat 702, an upper seat 703, a pin shaft 704, a return compression spring, a pressing block 706, a first pin 707, a U-shaped link 708, a handle 709, a magnet 710, a control handle 711, a third pin 712 and a second pin 713, the base 701 is fixed on a support of the oscillation driving device 5, the base 701 is provided with a lower seat 702 in an embedded mode, the lower seat 702 is provided with a first notch 7021, a horizontal shaft 7022 is arranged in the first notch 7021, the horizontal shaft 7022 is rotatably connected with a pin shaft 704, the lower seat 702 is connected with an upper seat 703 through the pin shaft 704, the upper seat is provided with a second notch 7031, the pin shaft 704 penetrates through the second notch 7031, the upper seat 703 is fixed on the clamp locking plate 6, a return pressure spring 705 is sleeved outside the pin shaft 704, the top end of the return pressure spring 705 is connected with a pressing block 706, the pressing block 706 is rotatably connected with a U-shaped link rod 708 through a first pin 707, the U-shaped link rod 708 is rotatably connected with a handle seat 709 through a second pin 713, the handle seat 709 is rotatably connected with the pin shaft 704 through a third pin 712, and the handle seat 709 is provided with a control handle 711;

an air duct 22 is arranged on the back of the inner cavity 3, a refrigerating device 9, a heating device 10, an air flow guiding and mixing device 11 and a fan 12 are sequentially arranged in the air duct 22 from bottom to top, a temperature detection sensor 13, a temperature recording sensor 14, a carbon dioxide sensor 15, an oxygen sensor 16 and a humidity sensor 17 are arranged at the lower part of the air duct 22, and a humidifying device 18 and a dehumidifying device 19 are arranged at the lower right side of the air duct 22.

When the culture apparatus of the embodiment is used, the box door 2 is opened, the handle 711 is moved upwards, after the resistance of the magnet 710 and the position of the 90-degree dead point are overcome, when the handle 711 is in the vertical state, the quick locking device 7 is in the open state, the quick locking device 7 is rotated by 90 degrees along the first gap 7021 and the second gap 7031, the clamp locking plate 6 is pulled out of the box door 2 along the guide rail 8, and then the culture dish is fixed on the clamp locking plate 6; after the culture dish is fixed, push away anchor clamps jam plate 6 and make it remove to the normal position along guide rail 8, rotate quick locking device 7 to first breach 7021 and second breach 7031 after again, push down handle 911, turn right down to the dead point when control handle 711 from vertical direction, drive anchor clamps jam plate 6 downstream, make upper seat 703 and lower seat 702 block play limiting displacement simultaneously, anchor clamps jam plate 6 is compressed tightly fast and makes it not shift and rock, best close chamber door 2, the start power supply, start cell culture.

Referring to fig. 1, 11 and 12, a superposition structure between a first box 101 and a second box 102 is specifically as follows, wherein four top corners of the bottom of the box 1 are respectively provided with a superposition device 20, and the superposition device 20 comprises a superposition inclined plate 2001, an inclined threaded hole top plate 2002, a threaded pipe 2003, a U-shaped plate 2004, a connecting piece 2005, an inverted U-shaped metal plate 2006 and a screw 2007; the left side of the overlapped sloping plate 2001 is fixed with a threaded pipe 2003 through a connecting piece 2005, the threaded pipe 2003 is fixed to an inverted U-shaped metal plate 2006 through a U-shaped plate 2004, the threaded pipe 2003 plays a role of fixing the overlapped sloping plate 2001, the right side of the overlapped sloping plate 2001 is screwed into a screw 2007, and the screw 2007 fixes the overlapped sloping plate 2001 and a sloping threaded hole top plate 2002 together, so that rapid overlapping between two box bodies is achieved; fig. 1 is a schematic diagram of two stacked boxes, and certainly, three or even more stacked boxes can be stacked, so that the function of compact structure is achieved.

As shown in fig. 1, 2 and 5, the door 2 includes a door shell sheet metal 201 and a visible plastic plate 202, the visible plastic plate 202 is embedded outside the door shell sheet metal 201, the visible plastic plate 202 is divided into a see-through prevention region and a transparent region, the see-through prevention region is a matte region formed by milling or chemically etching the peripheral surface of the visible plastic plate 202, and the transparent region is a convex region of the visible plastic plate 202; the refrigerator door 2 further comprises an annular heating rubber strip 203, an annular drying agent storage bin 204, a transparent plastic plate 205 and an insulating layer 206, the annular heating rubber strip 203 is arranged between the protruding area of the visible plastic plate 202 and the transparent plastic plate 205, the annular drying agent storage bin 204 is arranged on the inner ring of the annular heating rubber strip 203, the annular drying agent storage bin 204 is provided with a plurality of pores, and the insulating layer 26 is arranged between the anti-perspective area of the visible plastic plate 202 and the door shell metal plate 201; the fog surface area shields the internal structure of the box door 2, so that the box door has the functions of beauty and confidentiality; the protruding area of the visible plastic plate 202 is a transparent area, the annular drying agent storage bin 204 with the drying agent placed inside is arranged in the inner ring of the annular heating rubber strip 203, the annular heating rubber strip 203 plays a role in heating, so that gas between the protruding area of the visible plastic plate 202 and the transparent plastic plate is heated, the transparent area is heated, water mist generated in the transparent area of the visible plastic plate 202 is prevented, and the box door is simple in condensed water prevention structure, good in effect and low in cost.

As shown in fig. 1, fig. 2, fig. 3, fig. 4 and fig. 9, the air duct 22 is disposed at the back of the inner cavity 3, and the inside of the air duct 22 is sequentially provided with the refrigerating device 9, the heating device 10, the air flow guiding and mixing device 11 and the fan 12 from bottom to top, the air flow guiding and mixing device 11 includes a left flow guide plate 1101, a right flow guide plate 1102, a left flow distribution plate 1103 and a right flow distribution plate 1104, the left flow guide plate 1101 and the right flow guide plate 1102 are symmetrically disposed inside the air flow guiding and mixing device 11, the left flow guide plate 1103 and the right flow distribution plate 1104 are symmetrically disposed inside the air flow guiding and mixing device 11, and the left flow guide plate 1101 and the right flow guide plate 1102 are respectively located below the left flow distribution plate 1103 and the right flow distribution plate 1104, so that the cold and hot and cold air pass through the left flow guide plate 1101 and the right flow guide plate 1102, and the hot air form vortex mixing in the middle, and then cooperate with the left flow distribution plate 1103 and the right flow distribution plate 1104, so that the air is more uniformly mixed, and the layering of the air is prevented.

As shown in fig. 1, 2, 3, 4, 10, 11, 12 and 13, a temperature detecting sensor 13, a temperature recording sensor 14, a carbon dioxide sensor 15, an oxygen sensor 16 and a humidity sensor 17 are installed at the lower part of an air duct 22, a humidifying device 18 and a dehumidifying device 19 are installed at the lower right side of the air duct 22, the dehumidifying device 19 comprises an air suction pump 1901, a first water vapor filter 1902, a first electromagnetic valve 1903, a second electromagnetic valve 1904 and a heater 1906, one end of the air suction pump 1901 is connected with the first water vapor filter 1902, the other end of the air suction pump 1901 is connected with the first electromagnetic valve 1903, one end of the first electromagnetic valve 1903 is connected with a pipe from an inner cavity 3, a heater 1906 is arranged on a branch pipe between the first water vapor filter 1902 and the second electromagnetic valve 1904, the dehumidifying device 19 further comprises a moisture exhaust air pump 1905, a temperature sensor 1907, a moisture exhaust electromagnetic valve 1908 and a second water vapor filter 1909, the heater 1906 and the branch pipe are wound with the temperature sensor 1907, one end of the moisture exhaust air pump 1905 is connected with the moisture filter 1905, and one end of the moisture filter 1905 is connected with the moisture pump 1909; the drainage device 21 comprises a drainage nozzle 2101, a hose 2102, a round pipe 2103 and a U-shaped groove plate 2104, the outer wall of an inner cavity 3 is connected with the drainage nozzle 2101 in a penetrating manner, the end part of the drainage nozzle 2101 is fixedly connected with the hose 2102, the hose 2102 is clamped and fixed on the inner side of the round pipe 2103, meanwhile, the round pipe 2103 is connected with the U-shaped groove plate 2104 in a welding manner, the back part of the box 1 is provided with the drainage device 21, the drainage device 21 further comprises a water outlet hard pipe 2105 and a one-way valve 2106, the end part of the hose 2102 is fixedly connected with the water outlet hard pipe 2105, the one-way valve 2106 is arranged between the hose 2102 and the water outlet hard pipe 2105, the right side of the box 1 is provided with a humidifying water bucket hanging basket device 23, the humidifying water bucket hanging basket device 23 comprises a porous hanging basket 2301, a wave hanging frame 2302 and a reinforcing plate 2303, the porous hanging frame 2301 is fixedly connected with a wave 2302 through bolts, the reinforcing plate 2303 is welded on the back part of the wave hanging frame 2302, the porous hanging frame 2301 is beneficial to weight reduction and the beauty, and the reinforcing plate 2303 and the porous hanging frame 2303 is jointly improves the bearing capacity, so that the stability of the porous hanging frame 2301 is improved.

In summary, the superposable constant temperature and humidity three-gas oscillation cell culture device firstly has the structure shown in fig. 1-8, and the condensed water prevention working process of the device is as follows: the peripheral face of visual plastic slab 202 reaches the matte effect and prevents the perspective through milling process or chemical corrosion to utilize the relatively poor characteristic of heat-conduction of visual plastic slab 202, play better thermal-insulated effect, visual plastic slab 202 bellying is transparent region, the bellying of visual plastic slab 202 with be provided with annular heating rubber strip 203 between the transparent plastic slab 205, annular heating rubber strip 203 plays heating and sealed hollow gas's effect, is equipped with the annular drier containing bin 204 of inside placing the drier through at annular heating rubber strip 203 inner circle, annular heating rubber strip 203 plays the effect of heating, makes the protruding region of visual plastic slab 202 and the gas between the transparent plastic slab obtain the heating, produces the heating to transparent region, prevents that visual plastic slab transparent region from producing the water smoke, makes the anti-condensation water simple structure, and effectual, with low costs.

Referring to fig. 2, 6 to 8, the working principle of the quick locking and unlocking of the device is as follows: when the control handle 711 rotates ninety degrees from the vertical direction to the dead point, the clamp locking plate 6 is driven to move downwards, the upper seat 703 and the lower seat 702 are clamped to play a limiting role, and the clamp locking plate 6 is quickly pressed to prevent the clamp locking plate from shifting and shaking; after the handle 711 is lifted, the handle 711 and the quick mounting device 7 are rotated by 90 degrees along the first notch 7021 and the second notch 7031, so that the quick mounting device 7 is separated from the upper seat 703, and the clamp locking plate 6 is pulled out quickly; through the action, the quick locking and the loosening are realized, the precious time of scientific research personnel is saved, and compared with a thread locking mode, the problem that the threads are not screwed up or are not screwed into place and are easy to loosen is solved, and the reliability is better.

The uniform temperature control working process of the equipment is as follows: the hot and cold air entering the air duct 22 firstly needs to pass through the left baffle 1101 and the right baffle 1102, and through the cambered surface and the inclined symmetrical design of the left baffle 1101 and the right baffle 1102, the hot and cold air forms vortex mixing in the middle after passing through the left baffle 1101 and the right baffle 1102, the mixed air also needs to pass through the left splitter plate 1103 and the right splitter plate 1104, the left splitter plate 1103 and the right splitter plate 1104 are also designed in cambered surface and inclined symmetrical design, but the inclination direction is completely opposite to that of the left baffle 1101 and the right baffle 1102, so that further mixing can be realized;

the dehumidification working process of the device is as follows: the first electromagnetic valve 1903 and the second electromagnetic valve 1904 are in a normally open state, the moisture-removing electromagnetic valve 1908 is closed, the air suction pump 1901 works to generate negative pressure, moisture is sucked from the inner cavity 3, moisture is filtered through the first moisture filter 1902, and dry air enters the cavity to achieve the purpose of reducing the humidity of the air in the inner cavity 3. After the first dehumidification period is finished, in order to ensure a constant dehumidification effect, moisture in the moisture filter I1902 needs to be dehumidified, at the moment, the solenoid valve I1903 and the solenoid valve II 1904 are in a normally closed state, the dehumidification solenoid valve 1908 is opened, the suction pump 1901 does not work, the heater 1906 works to enable the pipeline to reach a preset temperature, then the dehumidification air pump 1905 operates, the dehumidification air pump 1905 sucks air from the outside air, the air is dried through the moisture filter II 1909, then the air is heated through the pipeline of the heater 1906 and then enters the moisture filter I1902, moisture on one side of the moisture filter I1902 is taken away reversely and is discharged through the pipeline of the dehumidification solenoid valve 1908, so that the moisture filter I1902 is ensured to be in a drying and constant maintaining effect, and the dehumidification inner cavity 3 is ensured to have a constant dehumidification function,

through this embodiment, realize the high accuracy humidity control requirement, specifically satisfy humidity control within 1.5%, humidity control range reaches 20% -95%, and this embodiment has humidification and dehumidification function simultaneously, makes cell culture device satisfy the cultivation demand of different cells.

The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims

1. A cell culture device capable of superposing three gases at constant temperature and humidity is characterized by comprising a box body (1) with an inner cavity (3) arranged inside, wherein the box body (1) is provided with a box door (2);

the inner bottom wall of the inner cavity (3) is provided with an oscillation driving device (5), the top of the oscillation driving device (5) is provided with a clamp locking plate (6), the clamp locking plate (6) is provided with a quick locking device (7) and a guide rail (8), the quick locking device (7) comprises a base (701), a lower seat (702), an upper seat (703), a pin shaft (704), a return pressure spring (705), a pressing block (706), a first pin (707), a U-shaped link rod (708), a handle seat (709), a magnet (710), a control handle (711), a third pin (712) and a second pin (713), the base (701) is fixed on a support of the oscillation driving device (5), the lower seat (702) is embedded in the base (701), a first notch (7021) is formed in the lower seat (702), a horizontal shaft (7022) is arranged in the first notch (7021), the horizontal shaft (7022) is rotatably connected with the pin shaft (704), the lower seat (702) is connected with the upper seat (703) through the pin shaft (704), a second pin shaft (70703) is arranged in the upper seat (703), the notch (70703) penetrates through the second pin shaft (7031), and the clamp locking plate (703) is sleeved with the outer side of the second pin shaft (703), the clamp locking plate (31), the top end of the return pressure spring (705) is connected with a pressing block (706), the pressing block (706) is rotatably connected with the U-shaped link rod (708) through a pin I (707), the U-shaped link rod (708) is rotatably connected with a handle seat (709) through a pin II (713), the handle seat (709) is rotatably connected with a pin shaft (704) through a pin III (712), and the handle seat (709) is provided with a control handle (711);

set up wind channel (22) at the back of inner chamber (3), just the inside in wind channel (22) is equipped with refrigerating plant (9), heating device (10), air current guiding and mixing device (11) and fan (12) from bottom to top in proper order to wind channel (22) lower part is equipped with temperature detect sensor (13), temperature record sensor (14), carbon dioxide sensor (15), oxygen sensor (16) and humidity transducer (17), wind channel (22) right side downside is equipped with humidification device (18) and dehydrating unit (19).

2. The cell culture apparatus according to claim 1, wherein the cell culture apparatus is a superposable cell culture apparatus,

the front surface of the box body (1) is provided with a box door (2) through a hinge structure, a heat resistance strip (4) is arranged between the inner cavity (3) and the box body (1), and the inner cavity (3) is contacted with the box body (1) through the heat resistance strip (4);

the bottom of the box body (1) is provided with a superposition device (20), the superposition device (20) comprises a superposition inclined plate (2001), an inclined threaded hole top plate (2002), a threaded pipe (2003), a U-shaped plate (2004), a connecting piece (2005), an inverted U-shaped metal plate (2006) and a screw (2007), the back of the box body (1) is provided with a drainage device (21), and the right side of the box body (1) is provided with a humidifying water bucket hanging basket device (23).

3. The superposable constant-temperature constant-humidity tri-gas oscillation cell culture device according to claim 1 or 2, wherein the box door (2) comprises a door shell metal plate (201) and a visual plastic plate (202), the visual plastic plate (202) is embedded in the outer side of the door shell metal plate (201), the visual plastic plate (202) is divided into an anti-perspective area and a transparent area, the anti-perspective area is a matte area formed by milling or chemically corroding the peripheral surface of the visual plastic plate (202), and the transparent area is a convex area of the visual plastic plate (202).

4. The superposable constant-temperature constant-humidity three-gas oscillation cell culture device according to claim 3, wherein the box door (2) further comprises an annular heating rubber strip (203), an annular drying agent storage bin (204), a transparent plastic plate (205) and an insulating layer (206), the annular heating rubber strip (203) is arranged between the convex area of the visible plastic plate (202) and the transparent plastic plate (205), the annular drying agent storage bin (204) is arranged on the inner ring of the annular heating rubber strip (203), the annular drying agent storage bin (204) is provided with a plurality of holes, and the anti-perspective area of the visible plastic plate (202) and the insulating layer (26) is arranged between the door shell metal plates (201).

5. The cell culture device capable of realizing superposition of constant temperature and humidity and three-gas oscillation according to claim 1, wherein the air flow guiding and mixing device (11) comprises a left guide plate (1101), a right guide plate (1102), a left splitter plate (1103) and a right splitter plate (1104), the left guide plate (1101) and the right guide plate (1102) are symmetrically arranged inside the air flow guiding and mixing device (11), the left splitter plate (1103) and the right splitter plate (1104) are symmetrically arranged inside the air flow guiding and mixing device (11), and the left guide plate (1101) and the right guide plate (1102) are respectively positioned below the left splitter plate (1103) and the right splitter plate (1104).

6. The cell culture device capable of superposing the constant temperature and humidity three-gas oscillation according to claim 1, wherein the dehumidifying device (19) comprises a getter pump (1901), a first water vapor filter (1902), a first electromagnetic valve (1903), a second electromagnetic valve (1904) and a heater (1906), one end of the getter pump (1901) is connected with the first water vapor filter (1902), the other end of the getter pump (1901) is connected with the first electromagnetic valve (1903), one end of the first electromagnetic valve (1903) is connected with a pipeline from the inner cavity (3), and the heater (1906) is arranged on a branch pipeline between the first water vapor filter (1902) and the second electromagnetic valve (1904);

dehydrating unit (19) is still including exhaust wet air pump (1905), temperature sensor (1907), exhaust wet solenoid valve (1908) and water vapor filter two (1909), and heater (1906) and lateral conduit arrange the winding have temperature sensor (1907), and the one end of heater (1906) is connected with exhaust wet air pump (1905), be provided with exhaust wet solenoid valve (1908) on the branch pipeline between aspirator pump (1901) and water vapor filter one (1902) moreover, the one end of exhaust wet air pump (1905) is connected with water vapor filter two (1909) simultaneously.

7. The superposable constant-temperature constant-humidity tri-gas oscillating cell culture device according to claim 2, wherein a slant threaded hole top plate (2002) is connected to the bottom of the superposition inclined plate (2001), a threaded pipe (2003) is welded above the superposition inclined plate (2001), a U-shaped plate (2004) is welded on the outer side of the threaded pipe (2003), a connecting piece (2005) is fixedly connected to the side of the U-shaped plate (2004), and the U-shaped plate (2004) and the connecting piece (2005) are fixed to the inverted U-shaped metal plate (2006).

8. The cell culture device capable of superposing the constant temperature and humidity three-gas oscillation according to claim 1, wherein the water drainage device (21) comprises a water drainage nozzle (2101), a hose (2102), a circular tube (2103) and a U-shaped groove plate (2104), the water drainage nozzle (2101) is connected to the outer wall of the inner cavity (3) in a penetrating manner, the hose (2102) is fixedly connected to the end of the water drainage nozzle (2101), the hose (2102) is clamped and fixed on the inner side of the circular tube (2103), and the circular tube (2103) is connected with the U-shaped groove plate (2104) in a welding manner.

9. The cell culture device capable of superposing the constant temperature and humidity three-gas oscillation according to claim 8, wherein the drainage device (21) further comprises a water outlet hard tube (2105) and a one-way valve (2106), the end of the hose (2102) is fixedly connected with the water outlet hard tube (2105), and the one-way valve (2106) is arranged between the hose (2102) and the water outlet hard tube (2105).

10. The cell culture device capable of superposing the constant temperature and humidity three-air vibration is characterized in that the humidifying water barrel hanging basket device (23) comprises a porous hanging basket (2301), a wave hanging frame (2302) and a reinforcing plate (2303), the porous hanging basket (2301) is fixedly connected with the wave hanging frame (2302) through bolts, and the reinforcing plate (2303) is welded at the back of the wave hanging frame (2302).