CN115850540A - Chromatographic activation coupling medium and preparation method thereof - Google Patents
Chromatographic activation coupling medium and preparation method thereof Download PDFInfo
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Abstract
The invention provides a chromatography activation coupling medium and a preparation method thereof. The chromatographic activation coupling medium takes polyacrylate microspheres as base spheres, and the polyacrylate microspheres have higher mechanical strength, so that the chromatographic column can bear higher pressure and 1MPa of pressure when filled, can still keep good separation effect after high pressure, and has longer service life.
Description
Technical Field
The invention relates to the field of biomedicine, in particular to a chromatography activation coupling medium and a preparation method thereof.
Background
The application of the biological medicine expands the research field of difficult diseases at present, so that the prior serious diseases threatening the life health of human beings can be effectively controlled. In the process of biological pharmacy, how to improve the purity and biological safety of medicines is a key step in the research and development and production processes of biological medicines such as recombinant proteins and antibodies.
The affinity chromatography activation coupling medium used in the market at present is mainly an agarose product, the mechanical strength of the chromatography medium is low, the improvement of the separation effect is limited due to low tolerance pressure when a chromatography column is filled in industrial production, and the service life of the chromatography medium is also limited.
Disclosure of Invention
The invention aims to solve the technical problem of providing a chromatographic activation coupling medium and a preparation method thereof, so as to solve the problem of low mechanical strength of the chromatographic activation coupling medium in the prior art.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a chromatography activation coupling medium takes polyacrylate microspheres as base spheres and has a structure shown as a formula (I):
preferably, the particle size of the polyacrylate microspheres is 50-90 microns.
The invention also provides a preparation method of the chromatography activation coupling medium, which comprises the following steps:
(1) Taking polyacrylate microspheres, adding allyl glycidyl ether, and reacting to obtain allylated microspheres;
(2) Adding bromine into the allylated microspheres obtained in the step (1) to react to obtain brominated microspheres;
(3) Adding 6-aminocaproic acid into the brominated microspheres obtained in the step (2) to react to obtain carboxylated microspheres;
(4) And (4) adding N-hydroxysuccinimide into the carboxylated microspheres obtained in the step (3) to react to obtain the chromatography activation coupling medium.
Preferably, step (1) specifically comprises: taking polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, and reacting for 1-2 hours at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain a first reactant; and then adding allyl glycidyl ether into the mixture, and reacting for 16-20h at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain the allylated microspheres.
Preferably, in the step (1), the weight ratio of the polyacrylate microspheres to the sodium sulfate to the sodium hydroxide to the sodium borohydride to the allyl glycidyl ether is 1000;
optionally, the reaction in step (1) is carried out with water as the reaction solvent.
Preferably, the step (2) specifically comprises: adding sodium acetate into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine, and reacting at 20-35 deg.C and stirring speed of 110-150rpm for 1-2h; then, sodium formate is added into the mixture to react to obtain the brominated microspheres.
Preferably, in the step (2), the weight ratio of the allylated microspheres to the sodium acetate is 1000 (2-8);
optionally, the weight ratio of the allylated microspheres to the bromine is 1000: (5-10); the weight ratio of the allylated microspheres to the sodium formate is 1000: (10-20);
optionally, the reaction in step (2) uses water as a reaction solvent.
Preferably, step (3) specifically comprises: adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 16-20h at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain carboxylated microspheres;
optionally, the aqueous solution of 6-aminocaproic acid is adjusted to pH 12 with NaOH;
optionally, the weight ratio of the brominated microspheres to the 6-aminocaproic acid is 1000 (80-110).
Preferably, the step (4) specifically comprises: and (4) adding N-hydroxysuccinimide and dicyclohexylcarbodiimide into the carboxylated microspheres obtained in the step (3), and reacting for 16-20h at the temperature of 20-35 ℃ and the stirring speed of 110-150rpm to obtain the chromatography activation coupling medium.
Preferably, in the step (4), the weight ratio of the carboxylated microspheres to the N-hydroxysuccinimide to the dicyclohexylcarbodiimide is 1000 (20-35) to (45-60);
optionally, the reaction in step (4) uses dichloromethane as the reaction solvent.
The scheme of the invention at least comprises the following beneficial effects:
the chromatography activation coupling medium takes polyacrylate microspheres as base spheres, and compounds shown as a formula (I) are bonded on the base spheres. The chromatography activation coupling medium takes polyacrylate microspheres as base spheres, and the polyacrylate microspheres have high mechanical strength, so that the chromatography activation coupling medium can bear higher pressure when being filled into a chromatography column, can bear the pressure of 1MPa, can still keep good separation effect after high pressure, and has longer service life.
More importantly, the compound bonded on the base sphere has N-hydroxysuccinimide, so that the compound can be covalently bonded with biological ligands containing primary amine groups, and can be further applied to immunoaffinity chromatography media.
In addition, since the small molecule ligand such as the specific short peptide also has a primary amine group, the chromatography activation coupling medium can also be coupled with the small molecule ligand such as the specific short peptide. Meanwhile, the activating medium with the N-hydroxysuccinimide group at the tail end has high affinity with the amino group on the affinity ligand, so that the reaction speed is high, the reaction efficiency is high, a stable amido bond is formed between the amino group on the affinity ligand and the activating medium, and the problem that the obtained affinity chromatography medium rarely leaks from the affinity ligand in the storage and chromatography processes is solved. One end of the 6-amino caproic acid is amino and can react with the brominated microspheres, and the other end of the 6-amino caproic acid is carboxyl and can perform coupling reaction with the N-hydroxysuccinimide. By adopting 6-aminocaproic acid to carry out coupling reaction of N-hydroxysuccinimide, the binding performance of the chromatography activation coupling medium obtained after the reaction and the affinity ligand is greatly improved.
Drawings
FIG. 1 is a standard curve of measurement of NHS concentration in Experimental example 2 for the effect of the present invention;
FIG. 2 is a standard curve of protein concentration measured in Experimental example 3 for the effect of the present invention.
Detailed Description
Those not indicated in the examples of the present invention were carried out under the conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, but are conventional products which can be obtained commercially, and the implementation of the technical scheme and the realization of the technical effect are not influenced by the raw materials of different manufacturers and models.
Example 1
The chromatography activation coupling medium in this embodiment uses polyacrylate microspheres as base spheres, and has a structure shown in formula (i):
the particle size of the polyacrylate microsphere is 70 microns.
The preparation method of the chromatography activation coupling medium described in this embodiment includes the following steps:
(1) Taking polyacrylate microspheres, adding allyl glycidyl ether, and reacting to obtain allylated microspheres;
(2) Adding bromine into the allylated microspheres obtained in the step (1) to react to obtain brominated microspheres;
(3) Adding 6-aminocaproic acid into the brominated microspheres obtained in the step (2) to react to obtain carboxylated microspheres;
(4) And (4) adding N-hydroxysuccinimide into the carboxylated microspheres obtained in the step (3) to react to obtain the chromatography activation coupling medium.
The preparation method of the chromatography activation coupling medium specifically comprises the following steps:
(1) Cleaning polyacrylate microspheres with deionized water 2 times the volume of the polyacrylate microspheres, soaking for 3min, draining, and repeating for 4 times to obtain cleaned polyacrylate microspheres;
taking the cleaned polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, taking water as a reaction solvent, dissolving the sodium hydroxide in the water, uniformly mixing the sodium hydroxide with the polyacrylate microspheres, the sodium sulfate and the sodium borohydride, and reacting for 1h at the temperature of 50 ℃ and the stirring speed of 130rpm to obtain a first reactant;
and then adding allyl glycidyl ether and water into the first reactant, and reacting for 16h at the temperature of 50 ℃ and the stirring speed of 130rpm to obtain the allylated microspheres.
Wherein the weight ratio of the polyacrylate microsphere to the sodium sulfate to the sodium hydroxide to the sodium borohydride to the allyl glycidyl ether is 1000. The weight ratio of water to the sodium hydroxide in preparing the first reactant is 1. In preparing allylated microspheres, the weight ratio of water to the allyl glycidyl ether was 3.
As a preferred implementation manner of this embodiment, the method further comprises a step of performing post-treatment on the allylated microsphere: and (3) carrying out suction filtration on the obtained reaction mixture containing the allylation microsphere, washing a filtrate with 5 times of volume of deionized water, 5 times of volume of 95V/V% ethanol, 2 times of volume of deionized water, 2 times of volume of 0.2mol/L acetic acid and 5 times of volume of deionized water in sequence, and carrying out suction drying to obtain the allylation microsphere.
(2) Adding sodium acetate and water into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine into the mixture, and reacting for 2 hours at the temperature of 20 ℃ and the stirring speed of 130 rpm; then, sodium formate is added into the mixture to react to obtain the brominated microspheres.
Wherein the weight ratio of the allylated microspheres to the sodium acetate is 1000; the weight ratio of the allylated microsphere to the bromine is 1000:10; the weight ratio of the allylated microspheres to the sodium formate is 1000:15.
as a preferred implementation manner of this embodiment, the method further comprises a step of performing post-treatment on the brominated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the brominated microspheres, washing the filtrate with deionized water, and carrying out suction drying to obtain the brominated microspheres.
(3) Adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 20 hours at the temperature of 40 ℃ and the stirring speed of 130rpm to obtain carboxylated microspheres;
wherein the aqueous solution of 6-aminocaproic acid is prepared by mixing 6-aminocaproic acid and water according to the weight ratio of 1:10, and adjusting the pH value to 12 by adopting NaOH; the weight ratio of the brominated microspheres to 6-aminocaproic acid is 1000.
As a preferred implementation manner of this embodiment, the method further comprises the step of performing post-treatment on the carboxylated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the carboxylated microspheres, sequentially washing the filtrate with deionized water, 0.2mol/L acetic acid solution and deionized water, and carrying out suction drying to obtain the carboxylated microspheres.
(4) Leaching the carboxylated microspheres obtained in the step (3) by adopting 2 times of volume of absolute ethyl alcohol, leaching by using 5 times of volume of dichloromethane until water is removed, and draining to obtain anhydrous carboxylated microspheres;
and adding N-hydroxysuccinimide, dicyclohexylcarbodiimide and dichloromethane into the carboxylated microspheres, and reacting for 16 hours at the temperature of 35 ℃ and the stirring speed of 130rpm to obtain the chromatography activation coupling medium.
Wherein the weight ratio of the carboxylated microspheres to the N-hydroxysuccinimide to the dicyclohexylcarbodiimide is 1000.
As a preferred implementation manner of the embodiment, the method further comprises the step of post-treating the chromatography-activated coupling medium: and (3) carrying out suction filtration on the obtained reaction mixture containing the chromatography activation coupling medium, washing the filtrate with dichloromethane with 5 times of volume and isopropanol with 5 times of volume in sequence, and carrying out suction drying to obtain the chromatography activation coupling medium.
Example 2
The chromatography activation coupling medium in this embodiment uses polyacrylate microspheres as base spheres, and has a structure shown in formula (i):
the particle size of the polyacrylate microspheres is 50 microns.
The preparation method of the chromatography activation coupling medium described in this embodiment specifically includes the following steps:
(1) Cleaning polyacrylate microspheres with deionized water of 2 times volume, soaking for 5min, draining, and repeating for 6 times to obtain cleaned polyacrylate microspheres;
taking the cleaned polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, taking water as a reaction solvent, dissolving the sodium hydroxide in the water, uniformly mixing the sodium hydroxide with the polyacrylate microspheres, the sodium sulfate and the sodium borohydride, and reacting for 2 hours at the temperature of 40 ℃ and the stirring speed of 110rpm to obtain a first reactant;
and then adding allyl glycidyl ether and water into the first reactant, and reacting for 20 hours at the temperature of 40 ℃ and the stirring speed of 150rpm to obtain the allylated microspheres.
Wherein the weight ratio of the polyacrylate microsphere to the sodium sulfate to the sodium hydroxide to the sodium borohydride to the allyl glycidyl ether is 1000. The weight ratio of water to the sodium hydroxide in preparing the first reactant is 1. In preparing allylated microspheres, the weight ratio of water to the allyl glycidyl ether was 3.
As a preferred implementation manner of this embodiment, the method further includes a step of performing post-treatment on the allylated microsphere: and (2) carrying out suction filtration on the obtained reaction mixture containing the allylated microspheres, washing the filtrate with 5 times of volume of deionized water, 5 times of volume of 95V/V% ethanol, 2 times of volume of deionized water, 2 times of volume of 0.2mol/L acetic acid and 5 times of volume of deionized water in sequence, and carrying out suction drying to obtain the allylated microspheres.
(2) Adding sodium acetate and water into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine into the mixture, and reacting for 1h at the temperature of 35 ℃ and the stirring speed of 110 rpm; then, sodium formate is added into the mixture to react to obtain the brominated microspheres.
Wherein the weight ratio of the allylated microspheres to the sodium acetate is 1000; the weight ratio of the allylated microspheres to the bromine is 1000:5; the weight ratio of the allylated microspheres to the sodium formate is 1000:10.
as a preferred implementation manner of this embodiment, the method further comprises a step of performing post-treatment on the brominated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the brominated microspheres, washing the filtrate with deionized water, and carrying out suction drying to obtain the brominated microspheres.
(3) Adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 16h at the temperature of 50 ℃ and the stirring speed of 110rpm to obtain carboxylated microspheres;
wherein the aqueous solution of 6-aminocaproic acid is prepared by mixing 6-aminocaproic acid and water according to the weight ratio of 1:10, and adjusting the pH value to 12 by adopting NaOH; the weight ratio of the brominated microspheres to 6-aminocaproic acid was 1000.
As a preferred implementation manner of this embodiment, the method further comprises the step of performing post-treatment on the carboxylated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the carboxylated microspheres, sequentially washing the filtrate with deionized water, 0.2mol/L acetic acid solution and deionized water, and carrying out suction drying to obtain the carboxylated microspheres.
(4) Leaching the carboxylated microspheres obtained in the step (3) by adopting 2 times of volume of absolute ethyl alcohol, leaching by using 5 times of volume of dichloromethane until water is removed, and draining to obtain anhydrous carboxylated microspheres;
and adding N-hydroxysuccinimide, dicyclohexylcarbodiimide and dichloromethane into the carboxylated microspheres, and reacting for 20 hours at the temperature of 20 ℃ and the stirring speed of 150rpm to obtain the chromatography activation coupling medium.
Wherein the weight ratio of the carboxylated microspheres to the N-hydroxysuccinimide to the dicyclohexylcarbodiimide is 1000.
As a preferred implementation manner of the embodiment, the method further comprises the step of post-treating the chromatography-activated coupling medium: and (3) carrying out suction filtration on the obtained reaction mixture containing the chromatography activation coupling medium, washing the filtrate with dichloromethane with 5 times of volume and isopropanol with 5 times of volume in sequence, and carrying out suction drying to obtain the chromatography activation coupling medium.
Example 3
The chromatography activation coupling medium in this embodiment uses polyacrylate microspheres as base spheres, and has a structure shown in formula (i):
the particle size of the polyacrylate microsphere is 90 microns.
The preparation method of the chromatography activation coupling medium described in this embodiment specifically includes the following steps:
(1) Cleaning polyacrylate microspheres with deionized water 2 times the volume of the polyacrylate microspheres, soaking for 4min, draining, and repeating for 5 times to obtain cleaned polyacrylate microspheres;
taking the cleaned polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, taking water as a reaction solvent, dissolving the sodium hydroxide in the water, uniformly mixing the sodium hydroxide with the polyacrylate microspheres, the sodium sulfate and the sodium borohydride, and reacting for 1h at the temperature of 45 ℃ and the stirring speed of 150rpm to obtain a first reactant;
and then adding allyl glycidyl ether and water into the first reactant, and reacting for 18h at the temperature of 45 ℃ and the stirring speed of 110rpm to obtain the allylated microspheres.
Wherein the weight ratio of the polyacrylate microsphere to the sodium sulfate to the sodium hydroxide to the sodium borohydride to the allyl glycidyl ether is 1000. The weight ratio of water to the sodium hydroxide in preparing the first reactant is 1. In the preparation of allylated microspheres, the weight ratio of water to the allyl glycidyl ether was 3.
As a preferred implementation manner of this embodiment, the method further comprises a step of performing post-treatment on the allylated microsphere: and (3) carrying out suction filtration on the obtained reaction mixture containing the allylation microsphere, washing a filtrate with 5 times of volume of deionized water, 5 times of volume of 95V/V% ethanol, 2 times of volume of deionized water, 2 times of volume of 0.2mol/L acetic acid and 5 times of volume of deionized water in sequence, and carrying out suction drying to obtain the allylation microsphere.
(2) Adding sodium acetate and water into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine into the mixture, and reacting for 2 hours at the temperature of 25 ℃ and the stirring speed of 150 rpm; then, sodium formate is added into the mixture to react to obtain the brominated microspheres.
Wherein the weight ratio of the allylated microsphere to the sodium acetate is 1000; the weight ratio of the allylated microsphere to the bromine is 1000:7; the weight ratio of the allylated microspheres to the sodium formate is 1000:20.
as a preferred implementation manner of this embodiment, the method further includes a step of performing post-treatment on the brominated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the brominated microspheres, washing the filtrate with deionized water, and carrying out suction drying to obtain the brominated microspheres.
(3) Adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 18h at the temperature of 45 ℃ and the stirring speed of 150rpm to obtain carboxylated microspheres;
wherein the aqueous solution of 6-aminocaproic acid is prepared by mixing 6-aminocaproic acid and water according to the weight ratio of 1:10, and adjusting the pH value to 12 by adopting NaOH; the weight ratio of the brominated microspheres to 6-aminocaproic acid is 1000.
As a preferred implementation manner of this embodiment, the method further comprises the step of performing post-treatment on the carboxylated microspheres: and (3) carrying out suction filtration on the obtained reaction mixture containing the carboxylated microspheres, sequentially washing the filtrate with deionized water, 0.2mol/L acetic acid solution and deionized water, and carrying out suction drying to obtain the carboxylated microspheres.
(4) Leaching the carboxylated microspheres obtained in the step (3) by adopting 2 times of volume of absolute ethyl alcohol, leaching by using 5 times of volume of dichloromethane until water is removed, and draining to obtain anhydrous carboxylated microspheres;
and adding N-hydroxysuccinimide, dicyclohexylcarbodiimide and dichloromethane into the carboxylated microspheres, and reacting for 18 hours at the temperature of 25 ℃ and the stirring speed of 110rpm to obtain the chromatography activation coupling medium.
Wherein the weight ratio of the carboxylated microspheres to the N-hydroxysuccinimide to the dicyclohexylcarbodiimide is 1000.
As a preferred implementation manner of the embodiment, the method further comprises the step of post-treating the chromatography-activated coupling medium: and (3) carrying out suction filtration on the obtained reaction mixture containing the chromatography activation coupling medium, washing the filtrate with dichloromethane with 5 times of volume and isopropanol with 5 times of volume in sequence, and carrying out suction drying to obtain the chromatography activation coupling medium.
Example 4
The chromatography activation coupling medium in this embodiment uses polyacrylate microspheres as base spheres, and has a structure shown in formula (i):
the particle size of the polyacrylate microsphere is 70 microns.
The preparation method of the chromatography activation coupling medium described in this embodiment specifically includes the following steps:
(1) Taking polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, taking water as a reaction solvent, dissolving the sodium hydroxide in the water, uniformly mixing the sodium hydroxide with the polyacrylate microspheres, the sodium sulfate and the sodium borohydride, and reacting for 1h at the temperature of 50 ℃ and the stirring speed of 130rpm to obtain a first reactant;
and then adding allyl glycidyl ether and water into the first reactant, and reacting for 16h at the temperature of 50 ℃ and the stirring speed of 130rpm to obtain the allylated microspheres.
Wherein the weight ratio of the polyacrylate microsphere to the sodium sulfate to the sodium hydroxide to the sodium borohydride to the allyl glycidyl ether is 1000. The weight ratio of water to the sodium hydroxide in preparing the first reactant is 1. In the preparation of allylated microspheres, the weight ratio of water to the allyl glycidyl ether was 3.
(2) Adding sodium acetate and water into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine into the mixture, and reacting for 2 hours at the temperature of 20 ℃ and the stirring speed of 130 rpm; then, sodium formate is added thereto, and the reaction is carried out to obtain brominated microspheres.
Wherein the weight ratio of the allylated microspheres to the sodium acetate is 1000; the weight ratio of the allylated microspheres to the bromine is 1000:10; the weight ratio of the allylated microspheres to the sodium formate is 1000:15.
(3) Adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 20 hours at the temperature of 40 ℃ and the stirring speed of 130rpm to obtain carboxylated microspheres;
wherein the aqueous solution of 6-aminocaproic acid is prepared by mixing 6-aminocaproic acid and water according to the proportion of 1:10, and adjusting the pH value to 12 by adopting NaOH; the weight ratio of the brominated microspheres to 6-aminocaproic acid was 1000.
(4) Leaching the carboxylated microspheres obtained in the step (3) by adopting 2 times of volume of absolute ethyl alcohol, leaching by adopting 5 times of volume of dichloromethane until water is removed, and draining to obtain anhydrous carboxylated microspheres;
and adding N-hydroxysuccinimide, dicyclohexylcarbodiimide and dichloromethane into the carboxylated microspheres, and reacting for 16 hours at the temperature of 35 ℃ and the stirring speed of 130rpm to obtain the chromatography activation coupling medium.
Wherein the weight ratio of the carboxylated microspheres to the N-hydroxysuccinimide to the dicyclohexylcarbodiimide is 1000.
Comparative example 1
In this comparative example, a chromatographically activated coupling medium was prepared using agarose as the base sphere and following the procedure and starting material of example 1.
Comparative example 2
In this comparative example, a chromatographically activated coupling medium was prepared according to the starting material and method of example 1, except that in step (3), the aqueous solution of 6-aminocaproic acid was replaced with an equal weight of 5-aminopentanoic acid.
Comparative example 3
In this comparative example, a chromatographically activated coupling medium was prepared according to the starting material and method of example 1, except that in step (3), the aqueous solution of 6-aminocaproic acid was replaced with an equal weight of 7-aminoheptanoic acid.
Comparative example 4
In this comparative example, a chromatographically activated coupling medium was prepared according to the starting materials and method of example 1, except that in step (1), the weight ratio of polyacrylate microspheres, sodium sulfate, sodium hydroxide, sodium borohydride, allyl glycidyl ether was 1000.
Comparative example 5
In this comparative example, a chromatographically activated coupling medium was prepared according to the starting materials and methods of example 1, except that in step (1), the weight ratio of the polyacrylate microsphere, sodium sulfate, sodium hydroxide, sodium borohydride, allyl glycidyl ether was 1000.
Experimental examples of Effect
To verify the technical effect of the chromatographically activated coupling medium of the invention, the following tests were carried out:
the chromatography activation coupling medium prepared in the examples 1-4 and the comparative examples 1-5 was used to test the pressure resistance, the ligand density, and the coupling rate with affinity ligands.
Effect experimental example 1: test of pressure resistance
An instrument/equipment
Circulating water vacuum pump, G3 sand core funnel pumping system, chromatography equipment, chromatography column (phi 1.60cm is multiplied by 20.00 cm)
Two steps
1. Sample processing
And (3) placing 20-25mL of filler to be tested in a G3 sand core funnel pumping and washing system with the specification of 50mL, pouring 20mL of deionized water, and starting a vacuum pump to pump and wash until no liquid drips from the sand core funnel within 1 min. This pump wash was repeated 5 times.
2. Sample loading column
And (4) assembling the column according to the standard operation procedure of pre-packed column filling, and controlling the height of the packed bed layer to be 10 +/-0.2 cm. 3, sample testing
1) Connecting the packed chromatographic column to a chromatographic device.
2) A constant flow rate Vx (mL/min) was set from 0 (V1 was 1mL/min, vx = x mL/min), and after the flow rate was maintained for 5min, the system display pressure Px (MPa) at this time was recorded.
3) The flow rate was increased continuously with a gradient of 1mL/min, and the procedure of 2) was repeated, and Px at the corresponding Vx was recorded.
4) Until the system pressure exceeded 1.2Mpa, the corresponding flow rate at this time was recorded as V (mL/min), i.e., the maximum flow rate. Or when the flow rate is increased at a certain moment, the system pressure is increased sharply, which indicates that the filler reaches the limit of pressure tolerance, and the flow rate at the previous moment is recorded as the maximum flow rate V, and the corresponding pressure is the maximum pressure P.
5) The column was bypassed and the background pressure Py of the instrument was recorded at the same flow rate Vx (mL/min) as above.
4. Calculation results
And calculating Vx and the corresponding column pressure Pc according to a formula. The formula is as follows:
1) Pc calculation formula of Vx corresponding to column pressure
P c ＝P x -P y
In the formula:
pc-the corresponding column pressure (MPa) at a flow rate Vx;
px-the corresponding system pressure (MPa) at a flow rate Vx;
py-the background pressure (MPa) of the corresponding instrument at a flow rate Vx.
Effect experimental example 2: NHS ligand Density determination
An instrument/equipment
Spectrophotometer, centrifuge, pipettor, circulating water type vacuum pump, sand core funnel
Two-reagent medicine
N-hydroxysuccinimide (NHS, mw 115.09) and concentrated aqueous ammonia
Three steps
1. Preparation of NHS Standard Curve
1) 0.1mol/L ammonia water is prepared
Accurately measure 0.667ml of concentrated ammonia (15M) and add to 99.333ml of deionized water and mix well.
2) An aqueous solution of 0.0002mol/L (0.2 mM) NHS standard was prepared
Accurately weighing 0.0575g of NHS solid, adding 5mL of deionized water to prepare 0.10mol/L NHS mother liquor; 0.020mL of mother liquor is measured, and deionized water of 9.980m is added to be mixed evenly.
3) Each sample was added to a 5mL centrifuge tube in the ratio shown in the table and mixed well.
4) Adding 1mL of sample into a quartz cuvette, and measuring the light absorption value A of 260nm by a spectrophotometer 260 . The final concentration of NHS and the absorbance A 260 Making a standard curve and fitting a standard curve formula A 260 ＝a×C NHS Wherein the standard curve of the NHS concentration determination is shown in fig. 1.
2. Determination of actual samples
And (3) pumping and washing the NHS medium to be detected for 5 times by using deionized water through a sand core funnel, weighing 0.5g (accurate to 0.001 g) of the washed and drained NHS medium, adding 3.0mL of 0.1mol/L ammonia water, uniformly mixing, standing overnight for 16h at room temperature, centrifuging (3000rpm, 5min), and taking the supernatant. Mixing 3.0mL of ammonia water and 0.5mL of supernatant, and measuring light absorption value A 260 The specific steps are basically consistent with the standard curve measurement.
3. Calculation of results
The ligand density of NHS mediators was calculated according to the following formula.
In the formula:
C NHS being NHS mediatorsLigand density, μmol/g;
A 260 the measured absorbance value of the supernatant at the wavelength of 260 nm;
a is a constant obtained by standard curve fitting;
m is the mass of the NHS medium, g.
Effect experimental example 3: measurement of coupling ratio of NHS mediator and affinity ligand
An apparatus/device
Constant-temperature water bath oscillation shaking table, circulating water type vacuum pump, sand core funnel, spectrophotometer and centrifugal machine
Two-reagent medicine
Concentrated hydrochloric acid, sodium bicarbonate, sodium chloride, coomassie brilliant blue G-250, ethanol (95%), phosphoric acid (85%), bovine serum albumin, lysozyme
Three steps
1. Preparing a Coomassie brilliant blue dye solution:
100mg of Coomassie brilliant blue G-250 is dissolved in 50ml of 95% ethanol, 100mL of 85% phosphoric acid is added after the dissolution, the volume is adjusted to 1L by pure water, and the mixture is filtered by filter paper for standby after overnight. (Standard Curve with bovine serum Albumin BSA solution after each preparation.)
And (3) standard curve preparation: 2mg/mL of bovine serum albumin BSA mother solution is accurately prepared for later use. BSA stocks were pipetted at 0.5mL, 1mL, 2mL, 2.5mL, and 4mL, respectively, and diluted to 10mL to give concentrations of 0.1mg/mL, 0.2mg/mL, 0.4mg/mL, 0.5mg/mL, and 0.8mg/mL, respectively. 3mL of Coomassie dye solution is placed in a glass cuvette and then a spectrophotometer is placed for background zero clearing, 75uL of the protein solution is added into the cuvette and is uniformly mixed, then the ultraviolet absorption value is read for 3min, and the step is repeated to measure the ultraviolet absorption value of the protein solution with different concentrations. A standard curve equation a = C a + b was obtained by plotting the protein concentration C and the ultraviolet absorption value a, and the standard curve of the measured protein concentration is shown in fig. 2.
2. Preparation of buffer A (1mMHCl, 2-8 ℃ pre-cooled)
Measuring 8uL of concentrated hydrochloric acid, dissolving the concentrated hydrochloric acid in 100mL of deionized water, uniformly mixing to obtain 1M HCl, dissolving 100uL1M HCl in 999.9mL of deionized water, uniformly mixing, and placing the buffer solution in a refrigerator at 2-8 ℃ for precooling.
3. Buffer B (0.1M NaHCO) was prepared 3 +0.5M NaCl，pH 8.3)
8.40g of sodium bicarbonate and 29.22g of sodium chloride were weighed out and dissolved in 1000mL of deionized water, and the pH was adjusted to 8.3 with HCl after dissolution.
4. Preparing a protein solution:
200mg of lysozyme protein was weighed and dissolved in 20mL of buffer (0.1M NaHCO) 3 + 0.5mnalc, ph 8.3) so that the final concentration of protein is 10mg/ml.
5. Coupling reaction
The NHS activating medium was washed 3-5 times with 4 ℃ pre-cooled 1mM HCl, drained and buffered (0.1M NaHCO) 3 +0.5m nacl, ph 8.3) 2 times;
adding 5mL of lysozyme protein solution into 5mL of the pretreated medium, keeping the reaction temperature constant at room temperature (25 ℃), and reacting for 12-14h;
after the reaction was stopped, the supernatant was centrifuged (3000rpm, 5min, room temperature), and the supernatant was collected.
6. Determination of supernatant protein concentration
Placing 3mL of Coomassie dye solution into a glass cuvette, placing a spectrophotometer, resetting the background, adding 75uL of lysozyme protein solution (10 mg/mL) into the cuvette, uniformly mixing, timing for 3min, and reading to obtain an ultraviolet absorption value A of a supernatant before reaction Front side 。
Repeating the above steps to detect reaction, collecting supernatant, and reading ultraviolet absorption value as absorption value A of supernatant after reaction Rear end 。
7. Calculation of results
Protein coupling efficiency was calculated according to the following formula:
in the formula:
w is the protein coupling efficiency;
A rear end The absorption value of the supernatant after the reaction;
A front part The absorption value of the supernatant before reaction;
a is the protein concentration standard curve equation coefficient;
b is the protein concentration standard curve equation coefficient.
The result of the effect experiment is as follows:
the results of the experiment are as follows:
according to the comparison between examples 1 to 4 and comparative examples 2 to 5 and comparative example 1, the pressure resistance of the obtained chromatography activation coupling medium is remarkably improved compared with that of agarose by using polyacrylate as the base sphere. According to the comparison between examples 1-4 and comparative examples 2-3, the invention adopts 6-aminocaproic acid to bond N-hydroxysuccinimide on the brominated microspheres, and the obtained chromatography activated coupling medium has no great influence on the ligand density, but has a significant influence on the performance of the chromatography activated coupling medium in combination with the affinity ligand. According to the comparison between examples 1-4 and comparative examples 4-5, the weight ratio of polyacrylate microspheres to allyl glycidyl ether has a great influence on the density of the ligand and the performance of binding with the affinity ligand, and when the amount of allyl glycidyl ether is too small, the density of the ligand is reduced; when the dosage of the allyl glycidyl ether is excessive, although the density of the ligand is improved to a certain degree, the performance of the ligand combined with the affinity ligand is obviously reduced. The chromatographically activated coupling medium of example 1 was excellent in pressure resistance, ligand density, and binding property with affinity ligands.
It will be appreciated by those skilled in the art that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the invention are intended to be embraced therein.
Claims (10)
1. A chromatography activation coupling medium is characterized in that polyacrylate microspheres are used as base spheres and have a structure shown as a formula (I):
2. the chromatographically-activated coupling medium of claim 1, wherein the polyacrylate microspheres have a particle size of 50-90 microns.
3. A method for preparing a chromatographic activated coupling medium, comprising the steps of:
(1) Taking polyacrylate microspheres, adding allyl glycidyl ether, and reacting to obtain allylated microspheres;
(2) Adding bromine into the allylated microspheres obtained in the step (1) to react to obtain brominated microspheres;
(3) Adding 6-aminocaproic acid into the brominated microspheres obtained in the step (2) to react to obtain carboxylated microspheres;
(4) And (4) adding N-hydroxysuccinimide into the carboxylated microspheres obtained in the step (3) to react to obtain the chromatography activation coupling medium.
4. The method for preparing a chromatographically-activated coupling medium according to claim 3, characterized in that step (1) comprises in particular: taking polyacrylate microspheres, sodium sulfate, sodium hydroxide and sodium borohydride, and reacting for 1-2 hours at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain a first reactant; then adding allyl glycidyl ether into the mixture, and reacting for 16-20h at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain the allylated microspheres.
5. The method for preparing chromatographic activation coupling medium according to claim 4, wherein in step (1), the weight ratio of the polyacrylate microsphere, sodium sulfate, sodium hydroxide, sodium borohydride and allyl glycidyl ether is 1000;
optionally, the reaction in step (1) uses water as a reaction solvent.
6. The method for preparing a chromatographically-activated coupling medium according to claim 3, wherein step (2) specifically comprises: adding sodium acetate into the allylated microspheres obtained in the step (1), and uniformly mixing; adding bromine into the mixture, and reacting for 1-2h at the temperature of 20-35 ℃ and the stirring speed of 110-150 rpm; then, sodium formate is added into the mixture to react to obtain the brominated microspheres.
7. The process for preparing chromatographic activated coupling medium according to claim 6, wherein in step (2), the weight ratio of allylated microsphere to sodium acetate is 1000 (2-8);
optionally, the weight ratio of the allylated microspheres to the bromine is 1000: (5-10); the weight ratio of the allylated microspheres to the sodium formate is 1000: (10-20);
optionally, the reaction in step (2) uses water as a reaction solvent.
8. The method for preparing a chromatographically-activated coupling medium according to claim 3, wherein step (3) specifically comprises: adding an aqueous solution of 6-aminocaproic acid into the brominated microspheres obtained in the step (2), and reacting for 16-20h at the temperature of 40-50 ℃ and the stirring speed of 110-150rpm to obtain carboxylated microspheres;
optionally, adjusting the pH of the aqueous solution of 6-aminocaproic acid to 12 with NaOH;
optionally, the weight ratio of the brominated microspheres to 6-aminocaproic acid is 1000 (80-110).
9. The method for preparing a chromatographically-activated coupling medium according to claim 3, wherein step (4) specifically includes: and (4) adding N-hydroxysuccinimide and dicyclohexylcarbodiimide into the carboxylated microspheres obtained in the step (3), and reacting for 16-20h at the temperature of 20-35 ℃ and the stirring speed of 110-150rpm to obtain the chromatography activation coupling medium.
10. The process for preparing a chromatographically activated coupling medium according to claim 9, wherein in step (4), the weight ratio of the carboxylated micro spheres, N-hydroxysuccinimide, and dicyclohexylcarbodiimide is 1000 (20-35) to (45-60);
optionally, the reaction in step (4) is carried out with dichloromethane as the reaction solvent.
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