CN110498751B - Imprinted template molecule and preparation method and application thereof - Google Patents
Imprinted template molecule and preparation method and application thereof Download PDFInfo
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- CN110498751B CN110498751B CN201910752131.8A CN201910752131A CN110498751B CN 110498751 B CN110498751 B CN 110498751B CN 201910752131 A CN201910752131 A CN 201910752131A CN 110498751 B CN110498751 B CN 110498751B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 108700019745 Disks Large Homolog 4 Proteins 0.000 claims abstract description 44
- 229920000344 molecularly imprinted polymer Polymers 0.000 claims abstract description 29
- 238000012216 screening Methods 0.000 claims abstract description 14
- 102100022397 Nitric oxide synthase, brain Human genes 0.000 claims abstract 5
- 101710111444 Nitric oxide synthase, brain Proteins 0.000 claims abstract 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 48
- 102000047174 Disks Large Homolog 4 Human genes 0.000 claims description 42
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 30
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
- 239000003112 inhibitor Substances 0.000 claims description 23
- 230000006916 protein interaction Effects 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 17
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 16
- WMGVPDQNPUQRND-UHFFFAOYSA-N (2-methylphenyl)acetonitrile Chemical compound CC1=CC=CC=C1CC#N WMGVPDQNPUQRND-UHFFFAOYSA-N 0.000 claims description 15
- 229920000642 polymer Polymers 0.000 claims description 15
- 238000006116 polymerization reaction Methods 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 15
- OZAIFHULBGXAKX-UHFFFAOYSA-N 2-(2-cyanopropan-2-yldiazenyl)-2-methylpropanenitrile Chemical compound N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 9
- 229910052681 coesite Inorganic materials 0.000 claims description 8
- 229910052906 cristobalite Inorganic materials 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- 229910052682 stishovite Inorganic materials 0.000 claims description 8
- 229910052905 tridymite Inorganic materials 0.000 claims description 8
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 claims description 7
- 239000011162 core material Substances 0.000 claims description 7
- 239000011258 core-shell material Substances 0.000 claims description 7
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 claims description 7
- 238000000967 suction filtration Methods 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000003431 cross linking reagent Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 6
- FUKUFMFMCZIRNT-UHFFFAOYSA-N hydron;methanol;chloride Chemical compound Cl.OC FUKUFMFMCZIRNT-UHFFFAOYSA-N 0.000 claims description 6
- 239000003999 initiator Substances 0.000 claims description 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 5
- RCPAZWISSAVDEA-UHFFFAOYSA-N 2-amino-3,5-dibromobenzaldehyde Chemical compound NC1=C(Br)C=C(Br)C=C1C=O RCPAZWISSAVDEA-UHFFFAOYSA-N 0.000 claims description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical class [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 239000012074 organic phase Substances 0.000 claims description 3
- 239000003208 petroleum Substances 0.000 claims description 3
- 238000004537 pulping Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- CAEWJEXPFKNBQL-UHFFFAOYSA-N prop-2-enyl carbonochloridate Chemical compound ClC(=O)OCC=C CAEWJEXPFKNBQL-UHFFFAOYSA-N 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- 102100022264 Disks large homolog 4 Human genes 0.000 abstract 2
- 102000006538 Nitric Oxide Synthase Type I Human genes 0.000 description 38
- 108010008858 Nitric Oxide Synthase Type I Proteins 0.000 description 38
- 238000001179 sorption measurement Methods 0.000 description 26
- 239000002122 magnetic nanoparticle Substances 0.000 description 13
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical class O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 10
- 239000000523 sample Substances 0.000 description 9
- -1 peroxynitrite anion Chemical class 0.000 description 8
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 6
- FZERHIULMFGESH-UHFFFAOYSA-N N-phenylacetamide Chemical compound CC(=O)NC1=CC=CC=C1 FZERHIULMFGESH-UHFFFAOYSA-N 0.000 description 6
- 125000005336 allyloxy group Chemical group 0.000 description 6
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 6
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 5
- ANNNBEZJTNCXHY-NSCUHMNNSA-N Isorhapontigenin Chemical compound C1=C(O)C(OC)=CC(\C=C\C=2C=C(O)C=C(O)C=2)=C1 ANNNBEZJTNCXHY-NSCUHMNNSA-N 0.000 description 4
- 108090001041 N-Methyl-D-Aspartate Receptors Proteins 0.000 description 4
- 102000004868 N-Methyl-D-Aspartate Receptors Human genes 0.000 description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229940125904 compound 1 Drugs 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000005160 1H NMR spectroscopy Methods 0.000 description 3
- 229960001413 acetanilide Drugs 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 238000000643 oven drying Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000005711 Benzoic acid Substances 0.000 description 2
- 201000006474 Brain Ischemia Diseases 0.000 description 2
- DOHWHRKZQBAPQV-UHFFFAOYSA-N C1=CC(=C(C(=C1)C(=O)O)N)CC2=C(C(=CC(=C2)Br)Br)N Chemical compound C1=CC(=C(C(=C1)C(=O)O)N)CC2=C(C(=CC(=C2)Br)Br)N DOHWHRKZQBAPQV-UHFFFAOYSA-N 0.000 description 2
- 206010008120 Cerebral ischaemia Diseases 0.000 description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 2
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 description 2
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 2
- 235000010233 benzoic acid Nutrition 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 230000002490 cerebral effect Effects 0.000 description 2
- 206010008118 cerebral infarction Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- QELUYTUMUWHWMC-UHFFFAOYSA-N edaravone Chemical compound O=C1CC(C)=NN1C1=CC=CC=C1 QELUYTUMUWHWMC-UHFFFAOYSA-N 0.000 description 2
- 229950009041 edaravone Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 229940016667 resveratrol Drugs 0.000 description 2
- 235000021283 resveratrol Nutrition 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- KFDVPJUYSDEJTH-UHFFFAOYSA-N 4-ethenylpyridine Chemical compound C=CC1=CC=NC=C1 KFDVPJUYSDEJTH-UHFFFAOYSA-N 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- HOKKHZGPKSLGJE-GSVOUGTGSA-N N-Methyl-D-aspartic acid Chemical compound CN[C@@H](C(O)=O)CC(O)=O HOKKHZGPKSLGJE-GSVOUGTGSA-N 0.000 description 1
- HOKKHZGPKSLGJE-UHFFFAOYSA-N N-methyl-D-aspartic acid Natural products CNC(C(O)=O)CC(O)=O HOKKHZGPKSLGJE-UHFFFAOYSA-N 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- AKZWRTCWNXHHFR-PDIZUQLASA-N [(3S)-oxolan-3-yl] N-[(2S,3S)-4-[(5S)-5-benzyl-3-[(2R)-2-carbamoyloxy-2,3-dihydro-1H-inden-1-yl]-4-oxo-3H-pyrrol-5-yl]-3-hydroxy-1-phenylbutan-2-yl]carbamate Chemical compound NC(=O)O[C@@H]1Cc2ccccc2C1C1C=N[C@](C[C@H](O)[C@H](Cc2ccccc2)NC(=O)O[C@H]2CCOC2)(Cc2ccccc2)C1=O AKZWRTCWNXHHFR-PDIZUQLASA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000012662 bulk polymerization Methods 0.000 description 1
- MYXJUONNAJPJJT-UHFFFAOYSA-N carbonochloridic acid;prop-1-ene Chemical compound CC=C.OC(Cl)=O MYXJUONNAJPJJT-UHFFFAOYSA-N 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000000749 co-immunoprecipitation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 101150069842 dlg4 gene Proteins 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 208000037906 ischaemic injury Diseases 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- YAEXATRESCQXFT-UHFFFAOYSA-N methyl 2-acetamido-4-aminobenzoate Chemical compound COC(=O)C1=CC=C(N)C=C1NC(C)=O YAEXATRESCQXFT-UHFFFAOYSA-N 0.000 description 1
- 229940095102 methyl benzoate Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 238000001225 nuclear magnetic resonance method Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- OCAAZRFBJBEVPS-UHFFFAOYSA-N prop-2-enyl carbamate Chemical class NC(=O)OCC=C OCAAZRFBJBEVPS-UHFFFAOYSA-N 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011257 shell material Substances 0.000 description 1
- 239000002210 silicon-based material Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
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- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/14—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof
- C07C227/18—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters
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- C07C249/00—Preparation of compounds containing nitrogen atoms doubly-bound to a carbon skeleton
- C07C249/02—Preparation of compounds containing nitrogen atoms doubly-bound to a carbon skeleton of compounds containing imino groups
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- C07C271/06—Esters of carbamic acids
- C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
- C07C271/26—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring
- C07C271/28—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring to a carbon atom of a non-condensed six-membered aromatic ring
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Abstract
An imprinting template molecule, a preparation method and an application thereof, wherein the structural characteristics of the template molecule are as shown in formula I:
the molecularly imprinted polymer prepared by the imprinted template molecule can highly specifically identify a compound with PSD-95/nNOS uncoupling effect, and can be used for high-throughput rapid screening of a PSD-95/nNOS uncoupling target compound.
Description
Technical Field
The invention belongs to the technical field of molecular imprinting polymers, and particularly relates to an imprinting template molecule and a preparation method and application thereof. The molecularly imprinted polymer can be used for identifying a compound with PSD-95/nNOS uncoupling action with high specificity, and can be used for quickly screening a PSD-95/nNOS protein interaction inhibitor with high flux.
Background
After cerebral ischemia, the pathological release of Nitric Oxide, which is over-activated by N-Methyl-D-aspartic acid (NMDA) receptors and synthesized by Neuronal Nitric Oxide Synthase (nNOS), is one of the major factors of Neuronal injury after cerebral ischemic injury. Postsynaptic Density Protein-95 (PSD-95) plays an important role in this pathway. In cerebral ischemia, NMDA receptor is over-activated to form NMDA/PSD-95/nNOS complex, NO is pathologically released, and superoxide anion (O)2 -) The reaction produces a more toxic peroxynitrite anion (ONOO)-) Resulting in damage to the neurons. Because NMDA receptor, PSD-95 and nNOS have a plurality of important physiological functions, the inhibition of the functions of the NMDA receptor, the PSD-95 and the nNOS can cause serious toxic and side effects. The uncoupling of PSD-95/nNOS can inhibit the pathological release of NO without affecting the functions of NMDA receptor, PSD-95 and nNOS. Literature reference4-N- (2-hydroxy-3, 5-dichlorobenzyl) aminosalicylic acid (ZL006, Nature Medicine, 2010, 16: 1439-. The chemical structures of ZL006, IC87201, ZL006-Br, ZL006-tb are as follows:
the activity evaluation of the PSD-95/nNOS protein interaction inhibitor can adopt a nuclear magnetic resonance method, wherein the method needs to respectively extract PSD-95 and nNOS, and judges the combination condition of a target compound and PSD-95 by measuring the change of N-H related spectrums of nuclear magnetic resonance before and after administration; or adopting a co-immunoprecipitation method to examine the difference of the enhancement degree of the binding strength of nNOS and PSD95 between the administration group and the blank group under the MCAO condition of the mice. Both methods are time-consuming and labor-consuming, and it is difficult to quickly and simply evaluate the activity of the PSD-95/nNOS protein interaction inhibitor. The molecularly imprinted polymer has strong specificity and high selectivity, and can be used as an artificial antibody for high-throughput rapid screening of target compounds (Journal of the American Chemical Society, 2007, 129: 1680-. The molecularly imprinted polymer obtained by adopting ZL006 as a template molecule has obvious adsorption on a PSD-95/nNOS protein interaction inhibitor ZL006 under low concentration, so that the molecularly imprinted polymer can be used for high-throughput rapid screening of the PSD-95/nNOS protein interaction inhibitor (ChemHysChem, 2016, 17: 893-901). Chinese invention patent 201019026093.4 reports a preparation method of a molecular imprinted polymer of a PSD-95/nNOS protein interaction inhibitor, and by utilizing the method, the molecular imprinted polymer with a certain specific recognition effect on the PSD-95/nNOS protein interaction inhibitor can be obtained.
The traditional bulk polymerization method for preparing MIP is simple in device and strong in universality, but the elution template is difficult to form due to uneven imprinting sites and too deep embedding. The surface imprinting technology can make the molecule recognition site of the imprinted polymer located on the accessible surface, and can quickly reach adsorption saturation, so that the imprinted polymer has attracted extensive attention. A variety of matrix materials can be used for preparing MIP by surface imprinting, wherein silicon-based materials are more and more favored by people besides good mechanical properties, compatibility and thermal stability, and gradually become one of the preferred matrix materials for preparing surface-imprinted polymers. And is widely used (Food Chemistry 2012, 131: 1063-.
Generally, methods such as centrifugal separation and the like are needed for adsorption, cleaning and desorption of the molecularly imprinted polymer, and the separation process is complicated. The magnetic separation technology is characterized in that nano or micron magnetic particles are used as carriers, molecularly imprinted polymers with core-shell structures are adopted, and core materials are surface modified Fe3O4@SiO2Under the directional control of an external magnetic field, the magnetic nanoparticles have the advantages of simple and convenient magnetic separation, high specificity and high sensitivity of affinity adsorption and the like through the operations of adsorption, cleaning and desorption (Journal of Hazardous Materials 2011, 191:177 and 183).
Although the molecularly imprinted polymer obtained by using ZL006 as a template molecule can have a relatively good adsorption effect on a PSD-95/nNOS protein interaction inhibitor, the specificity is still unsatisfactory.
Furthermore, some analogues of ZL006, such as ZL006-Br, ZL006-tb, have a relatively large group at the 5-position of the lipophilic ring, and thus there is a risk of reduced selectivity for molecules having a relatively large group at the 5-position of the lipophilic ring, such as ZL006-Br, ZL006-tb, using ZL006 as a template molecule.
Disclosure of Invention
The technical problem to be solved is as follows: in order to overcome the defects of the prior art, the invention provides an imprinted template molecule and a preparation method and application thereof, and a molecularly imprinted polymer prepared by the imprinted template molecule can highly specifically identify a compound with PSD-95/nNOS uncoupling action and can be used for high-throughput rapid screening of a PSD-95/nNOS uncoupling target compound.
The technical scheme is as follows: an imprinted template molecule, wherein the imprinted template molecule has structural features shown in figure I:
the preparation method of the imprinted template molecule comprises the following steps: adding 5mmol of 2-acetamido-4-methyl aminobenzoate and 5mmol of 2-amino-3, 5-dibromobenzaldehyde into a reaction vessel according to the proportion, adding 800mL of ethanol and 10mL of formic acid, heating to reflux, reacting for 8h, cooling to room temperature, performing suction filtration, and washing with ethanol to obtain a bright yellow solid. Dissolving the bright yellow solid in 300mL of methanol and 500mL of 5M NaOH, reacting for 30h at 90 ℃, detecting complete reaction by TCL, recovering the solvent to 400mL under reduced pressure, adjusting the pH value to 6, performing suction filtration, and recrystallizing the filter residue with ethanol to obtain a white solid. Putting the white solid of the product in 150mL of acetonitrile, adding 36mmol of NaH at 0 ℃, stirring for 30min, adding 36mmol of allyl chloroformate, reacting for 4-5h at 60 ℃, detecting complete reaction by TCL, adding 200mL of water, extracting for three times by using 100mL of ethyl acetate, washing for three times by using 100mL of saturated sodium bicarbonate and 100mL of saturated saline solution respectively, collecting an organic phase, and pulping by using ethyl acetate and petroleum ether to obtain the imprinted template molecule.
The application of the imprinted template molecule in preparing the molecularly imprinted polymer comprises the following steps: a. 1.0g of surface amino group-modified Fe as a core material3O4@SiO22.0g of imprinting template molecules of the formula (I) are dispersed in 50mL of toluene-acetonitrile, wherein the volume ratio of toluene is 0-50%, and the mixture is stirred at room temperature for 1h for prepolymerization; adsorbing the solution at the bottom of a bottle by using a magnet, pouring out supernatant, and adding 50mL of toluene-acetonitrile, wherein the volume ratio of toluene is 0-50%, so as to obtain a pre-polymerization solution; b. adding 6.0g of crosslinking agent ethylene glycol dimethacrylate and 0.3g of initiator azobisisobutyronitrile into the prepolymerization liquid, introducing nitrogen to remove oxygen, sealing, and carrying out polymerization reaction in a heating and stirring manner to generate powdery polymer MIPs, wherein the polymerization reaction temperature is 55 ℃ and the reaction time is 36 hours; c. repeatedly ultrasonically eluting the obtained MIPs with 1moL/L hydrochloric acid-methanol solution to remove template molecules, and then usingAnd (3) washing with methanol to be neutral, and drying the polymer in a vacuum drying oven to be balanced in weight to obtain the molecularly imprinted polymer MIPs with the core-shell structure.
The imprinted template molecule is applied to high-throughput rapid screening of a PSD-95/nNOS protein interaction inhibitor.
The imprinted template molecule is applied to the preparation of a PSD-95/nNOS protein interaction inhibitor high-throughput rapid screening kit.
The prepared molecularly imprinted polymer is applied to the preparation of a PSD-95/nNOS protein interaction inhibitor high-throughput rapid screening kit.
Has the advantages that: the molecularly imprinted polymer prepared by the imprinted template molecule can highly specifically identify a compound with PSD-95/nNOS uncoupling effect, and can be used for high-throughput rapid screening of a PSD-95/nNOS uncoupling target compound.
Drawings
FIG. 1 is a schematic diagram of a PSD-95/nNOS protein interaction inhibitor highly specific artificial antibody;
FIG. 2 is a diagram showing the results of a PSD-95/nNOS protein interaction inhibitor high-specificity artificial antibody screening PSD-95/nNOS uncoupling target compound test.
Detailed Description
The following examples are given to enable those skilled in the art to fully understand the present invention, but are not intended to limit the invention in any way.
In order to avoid false positive, the invention firstly changes-Cl of ZL006 into-Br, then changes hydroxyl thereof into amino, adopts the form of carbamate, is combined with the allyl group, fixes the combination site in the form of chemical bond, and the residual acidic carboxyl can be combined with Fe modified by surface amino3O4@SiO2The magnetic nanoparticles have better combination. Fe3O4@SiO2The introduction of the magnetic nanoparticles can conveniently adopt magnet separation, thereby facilitating drug screening. The obtained molecularly imprinted polymer can highly specifically identify a PSD-95/nNOS protein interaction inhibitor, and can be used for high-throughput rapid screening of the PSD-95/nNOS protein interaction inhibitor, as shown in figure 1.
In order to eliminate the influence of nonspecific binding of the molecularly imprinted polymer, the invention introduces a high-concentration pseudo PSD-95/nNOS protein interaction inhibitor to inhibit nonspecific binding when determining the specificity of the molecularly imprinted polymer, and experimental results show that the specificity of the molecularly imprinted polymer to the PSD-95/nNOS protein interaction inhibitor can be obviously improved after the pseudo PSD-95/nNOS protein interaction inhibitor is added.
The invention provides a Molecularly Imprinted Polymer (MIPs) with high specificity recognition effect on a PSD-95/nNOS protein interaction inhibitor, wherein a template molecule of the MIP is a 4-N- (3, 5-dibromo-2-aminobenzyl) amino-2-aminobenzoic acid allyl carbamate derivative, and the MIP has the structural characteristics as shown in a figure I:
the PSD-95/nNOS protein interaction inhibitor high-specificity artificial antibody adopts a molecularly imprinted polymer with a core-shell structure, and the core material is Fe with surface amino modified3O4@SiO2The shell material is a molecularly imprinted polymer taking a carbamic acid allyl ester derivative (compound 1) of 4-N- (3, 5-dibromo-2-aminobenzyl) amino-2-aminobenzoic acid as a template molecule.
Example 1: synthesis of template molecule 4- (((allyloxy) carbonyl) (2- (((allyloxy) carbonyl) amino) -3, 5-dibromobenzyl) amino) -2- (((allyloxy) carbonyl) amino) benzoic acid (I)
1.1 synthetic route:
1.22 Synthesis of methyl-acetamido-4-N (2-amino-3, 5-dibromobenzyl) aminobenzoate (5):
10.4g (3, 50mmol) of methyl 2-acetamido-4-aminobenzoate and 13.8g (2, 50mmol) of 2-amino-3, 5-dibromobenzaldehyde are added into a 250mL eggplant-shaped bottle, 800mL of ethanol and 10mL of formic acid are heated to reflux, after reaction is carried out for 8 hours, a large amount of yellow solid is separated out after cooling to room temperature, suction filtration is carried out, a small amount of ethanol is used for washing, and 1.67g of bright yellow solid (4) is obtained, wherein the yield is 71.7%.
1H NMR(400MHz,Chloroform-d)δ8.56(d,J＝2.0Hz,1H),8.42(s,1H),8.06(d,J＝8.6Hz,1H),7.63(d,J＝2.3Hz,1H),7.45(d,J＝2.3Hz,1H),6.87(dd,J＝8.6,2.2Hz,1H),3.94(s,3H),2.26(s,3H).
And (3) putting the product (4) in 30mL of ethanol, adding 6.77g of sodium borohydride, stirring at normal temperature for 36h, detecting complete reaction by TCL, adding a proper amount of water, performing suction filtration, and drying to obtain 16.0g of yellow solid (5) with the yield of 95.2%.
Synthesis of 32-amino-4-N (2-amino-3, 5-dibromobenzyl) aminobenzoic acid (6):
and (3) reacting 16.0g of the product in 500mL of 5M NaOH in 300mL of methanol at 90 ℃ for 30h, detecting that the reaction is complete by TCL, adjusting the pH value to 6, performing suction filtration, and recrystallizing filter residues by using ethanol to obtain 7.8g of white solid (6), wherein the yield is 55.3%.
1H NMR(400MHz,DMSO-d6)δ7.43(d,J＝2.3Hz,1H),7.39(d,J＝8.8Hz,1H),7.06(d,J＝2.2Hz,1H),6.61(t,J＝6.0Hz,1H),5.83(dd,J＝8.7,2.2Hz,1H),5.64(d,J＝2.2Hz,1H),5.30(s,2H),4.07(d,J＝5.9Hz,2H).
1.44 Synthesis of- (((allyloxy) carbonyl) (2- (((allyloxy) carbonyl) amino) -3, 5-dibromobenzyl) amino) -2- (((allyloxy) carbonyl) amino) benzoic acid (I):
putting the white solid (4.1g, 10mmol) of the product into acetonitrile (150mL), adding NaH (0.90g, 36mmol) at 0 ℃, stirring for 30min, adding propylene chloroformate (4.3g,36mmol), reacting for 4-5h at 60 ℃, detecting the reaction completion by TCL, adding 200mL of water, extracting with ethyl acetate 100mL for three times, washing with 100mL of saturated sodium bicarbonate and 100mL of saturated saline solution for three times, collecting the organic phase, and pulping with ethyl acetate and petroleum ether to obtain 2.0g of white solid with the yield of 30%.
1H NMR(400MHz,DMSO-d6)δ7.88–7.75(m,1H),7.53(t,J＝5.9Hz,1H),7.47(d,J＝2.3Hz,1H),7.14(d,J＝2.3Hz,1H),6.64(d,J＝2.3Hz,1H),5.92(ddt,J＝16.3,10.9,5.6Hz,1H),5.82–5.60(m,2H),5.40–5.34(m,2H),5.27(d,J＝10.5Hz,1H),5.15–4.97(m,4H),4.72(d,J＝5.6Hz,2H),4.51(t,J＝6.2Hz,4H),4.24(d,J＝5.7Hz,1H).
13C NMR(101MHz,DMSO-D6)δ159.17,155.02,151.44,149.56,143.06,134.75,132.96,132.40,132.36,131.71,129.73,125.49,119.81,117.63,117.52,109.81,109.43,107.13,69.97,66.95,43.21,.
Example 2: preparation of MIPs
2.1 surface amino-modified Fe3O4@SiO2Preparation of magnetic nanoparticles
2.1.1 Fe3O4@SiO2-1 magnetic nanoparticles. 0.442g of 3-Aminopropyltriethoxysilane (APS) is added to 200mL of water and ethanol [ V (water): V (ethanol) ═ 1: 5%]After stirring at room temperature for 30min, 2g of Fe was added3O4Stirring the nano particles at room temperature for 3 hours, adjusting the pH value to 9-10, and continuously reacting for 3 hours; adsorbing with magnet at the bottom of the bottle, decanting the supernatant, adding appropriate amount of anhydrous ethanol, washing for 3 times, and oven drying at 80 deg.C to obtain surface amino modified Fe3O4@SiO2-1 magnetic nanoparticles.
2.1.2 Fe3O4@SiO2-2 magnetic nanoparticles. 0.884g of 3-Aminopropyltriethoxysilane (APS) is added to 200mL of water and ethanol [ V (water): V (ethanol) ═ 1: 5%]After stirring at room temperature for 30min, 2g of Fe was added3O4Stirring the nano particles at room temperature for 3 hours, adjusting the pH value to 9-10, and continuously reacting for 3 hours; adsorbing with magnet at the bottom of the bottle, decanting the supernatant, adding appropriate amount of anhydrous ethanol, washing for 3 times, and oven drying at 80 deg.C to obtain surface amino modified Fe3O4@SiO2-2 magnetic nanoparticles.
2.1.3 Fe3O4@SiO2-3 magnetic nanoparticles. 1.33g of 3-Aminopropyltriethoxysilane (APS) are added to 200mL of water and ethanol [ V (water): V (ethanol) ═ 1: 5%]After stirring at room temperature for 30min, 2g of Fe was added3O4Stirring the nano particles at room temperature for 3 hours, adjusting the pH value to 9-10, and continuously reactingThe reaction time is 3 hours; adsorbing with magnet at the bottom of the bottle, decanting the supernatant, adding appropriate amount of anhydrous ethanol, washing for 3 times, and oven drying at 80 deg.C to obtain surface amino modified Fe3O4@SiO2-3 magnetic nanoparticles.
2.2: preparation of MIPs
2.2.1 preparation of MIPs-1
a. 1.0g of surface amino group-modified Fe as a core material3O4@SiO2-1 magnetic nanoparticle, 2.0g template molecule 4-N- (3, 5-dibromo-2-aminobenzyl) amino-2-aminobenzoic acid carbamate allyl ester derivative (compound 1) dispersed in 50mL toluene-acetonitrile (containing toluene 0% -50%, volume ratio), room temperature stirring 1h prepolymerization; adsorbing at the bottom of the bottle by using a magnet, pouring out supernatant, and adding 50mL of toluene-acetonitrile (wherein the toluene content is 0-50%, and the volume ratio) to obtain a pre-polymerization solution.
b. The prepolymerized solution was charged with a crosslinking agent (ethylene glycol dimethacrylate, 6.0g) and an initiator (azobisisobutyronitrile, 0.3g), and sealed after removing oxygen by introducing nitrogen. And carrying out polymerization reaction by adopting a heating and stirring mode to generate powdery polymer MIPs. The polymerization temperature is 55 ℃, and the reaction time is 36 h;
c. and (3) repeatedly carrying out ultrasonic elution on the obtained MIPs by using 1moL/L hydrochloric acid-methanol solution to remove template molecules, then washing the MIPs to be neutral by using methanol, and drying the polymer in a vacuum drying oven until the weight is balanced to obtain the molecularly imprinted polymer MIPs-1 with the core-shell structure.
2.2.2 preparation of MIPs-2
a. 1.0g of surface amino group-modified Fe as a core material3O4@SiO22 magnetic nanoparticles, 2.0g of a template molecule, namely a carbamate derivative (compound 1) of 4-N- (3, 5-dibromo-2-aminobenzyl) amino-2-aminobenzoic acid, is dispersed in 50mL of toluene-acetonitrile (wherein the toluene content is 0-50%, and the volume ratio is), and stirred at room temperature for 1h for prepolymerization; adsorbing at the bottom of the bottle by using a magnet, pouring out supernatant, and adding 50mL of toluene-acetonitrile (wherein the toluene content is 0-50%, and the volume ratio) to obtain a pre-polymerization solution.
b. The prepolymerized solution was charged with a crosslinking agent (ethylene glycol dimethacrylate, 6.0g) and an initiator (azobisisobutyronitrile, 0.3g), and sealed after removing oxygen by introducing nitrogen. And carrying out polymerization reaction by adopting a heating and stirring mode to generate powdery polymer MIPs. The polymerization temperature is 55 ℃, and the reaction time is 36 h;
c. and (3) repeatedly carrying out ultrasonic elution on the obtained MIPs by using 1moL/L hydrochloric acid-methanol solution to remove template molecules, then washing the MIPs to be neutral by using methanol, and drying the polymer in a vacuum drying oven until the weight is balanced to obtain the molecularly imprinted polymer MIPs-1 with the core-shell structure.
2.2.3 preparation of MIPs-3
a. 1.0g of surface amino group-modified Fe as a core material3O4@SiO2-3 magnetic nanoparticles, 2.0g of a template molecule 4-N- (3, 5-dibromo-2-aminobenzyl) amino-2-aminobenzoic acid allyl carbamate derivative (compound 1) is dispersed in 50mL of toluene-acetonitrile (wherein the toluene content is 0-50%, volume ratio), and stirred at room temperature for 1h for prepolymerization; adsorbing at the bottom of the bottle by using a magnet, pouring out supernatant, and adding 50mL of toluene-acetonitrile (wherein the toluene content is 0-50%, and the volume ratio) to obtain a pre-polymerization solution.
b. The prepolymerized solution was charged with a crosslinking agent (ethylene glycol dimethacrylate, 6.0g) and an initiator (azobisisobutyronitrile, 0.3g), and sealed after removing oxygen by introducing nitrogen. And carrying out polymerization reaction by adopting a heating and stirring mode to generate powdery polymer MIPs. The polymerization temperature is 55 ℃, and the reaction time is 36 h;
c. and (3) repeatedly carrying out ultrasonic elution on the obtained MIPs by using 1moL/L hydrochloric acid-methanol solution to remove template molecules, then washing the MIPs to be neutral by using methanol, and drying the polymer in a vacuum drying oven until the weight is balanced to obtain the molecularly imprinted polymer MIPs-1 with the core-shell structure.
2.2.4 preparation of MIPs-4
The Chinese patent 201019026093.4 is referred to for preparation: taking about 0.67g (1mmol) of template molecule I and 1.0mmol of functional monomer 4-vinylpyridine, uniformly dispersing in 200mL of toluene-acetonitrile (1:9, v/v), stirring at room temperature, and prepolymerizing for 1 h; then adding 3mmol of crosslinking agent Ethylene Glycol Dimethacrylate (EGDMA) and 0.2g of initiator Azobisisobutyronitrile (AIBN), introducing nitrogen for 15min to remove oxygen, sealing, heating to 50 ℃, and reacting for 6 h; then heating to 60 ℃ for reaction for 24 h; finally, the reaction was completed for 6 hours at 85 ℃. And (3) repeatedly carrying out ultrasonic elution on the obtained MIPs by using a 1M hydrochloric acid-methanol solution to remove template molecules, then washing the MIPs to be neutral by using methanol, and carrying out vacuum drying on the polymer for later use (MIPs-4).
Example 3: measurement of adsorption amount
10mg of MIPs-1 are dispersed in 10mL of a toluene-acetonitrile (9:1, v/v) solution. 1.0mL of the suspension was mixed with 1.0mL of a toluene-acetonitrile (9:1, v/v) solution of 4-N- (2-hydroxy-3, 5-dichlorobenzyl) aminosalicylic acid (ZL006, 0.2mmol/L) (equivalent to an initial concentration of 0.1mmol/L), shaken in a constant temperature shaker at room temperature for 1 hour, and then separated, and the supernatant was taken, and the concentration of 4-N- (2-hydroxy-3, 5-dibromobenzyl) aminosalicylic acid in the sample solution before and after adsorption was measured by HPLC-UV to calculate the amount of adsorption. The adsorption quantity of ZL006 in MIPs-2, MIPs-3 and MIPs-4 is measured by the same method. The amount of acetanilide adsorbed at an initial concentration of 0.1mmol/L was determined in the same manner and compared with that of ZL 006.
As can be seen from the figure: the initial concentration of ZL006 is 0.1mmol/L, and the adsorption capacity of the MIPs-1 group, the MIPs-2 group, the MIPs-3 group and the MIPs-4 group is obviously higher than that of acetanilide 0.1mmol/L, and the MIPs-1 group, the MIPs-2 group and the MIPs-3 group are obviously higher than that of the control MIPs-4 group. And (4) prompting: the molecularly imprinted polymer obtained by the invention has higher specific recognition on ZL006 which is a compound representing PSD-95/nNOS uncoupling action.
Example 4: selective adsorption test
10mg of MIPs-1 are dispersed in 10mL of a toluene-acetonitrile (9:1, v/v) solution. Mixing 1.0mL of the suspension with 1.0mL of a sample (0.2mmol/L) + ZL006(0.2mmol/L) + acetanilide (4.0mmol/L) in toluene-acetonitrile (9:1, v/v) solution, shaking for 1h at room temperature in a constant temperature oscillator, centrifuging, collecting the supernatant, measuring the concentrations of the sample, ZL006 and methyl benzoate in the sample solution before and after adsorption by HPLC-UV, calculating the adsorption amount of MIPs to the sample and ZL006 according to the change of the solution concentration before and after the binding, dividing the adsorption amount of the sample by the adsorption amount of ZL006, and calculating the relative adsorption amount of the sample according to the adsorption amount of ZL006 being 100%. The relative adsorption amount of the sample is X100% of the adsorption amount of sample/adsorption amount of ZL006
And measuring the relative adsorption quantity of the MIPs-2, the MIPs-3 and the MIPs-4 to the sample by the same method.
TABLE 1 Selective adsorption test (sample relative adsorption ZL006 as 100%)
| IC87201 | ZL006-Br | ZL006-tb | Edaravone | Resveratrol | Isorhapontigenin | |
| MIPs-1 | 90.2 | 106.6 | 102.7 | 23.9 | 28.3 | 22.1 |
| MIPs-2 | 92.4 | 109.1 | 102.7 | 20.3 | 24.6 | 21.4 |
| MIPs-3 | 91.6 | 103.3 | 102.7 | 22.6 | 26.9 | 23.2 |
| MIPs-4 | 92.7 | 90.7 | 92.2 | 62.7 | 64.2 | 60.8 |
The results show that: MIPs-1, MIPs-2 and MIPs-3 have good relative adsorption effect on PSD-95/nNOS uncoupling molecules IC87201, ZL006-Br and ZL006-tb, the relative adsorption quantity is far larger than that of other therapeutic drugs for cerebral apoplexy such as free radical scavenger edaravone, resveratrol and isorhapontigenin, and the selectivity is obviously higher than that of MIPs-4. The MIPs have good specific recognition effect on PSD-95/nNOS uncoupling molecules.
Claims (6)
1. An imprinted template molecule characterized by the structural features of formula (I):
2. a method for preparing the imprinted template molecule of claim 1, characterized by the steps of: adding 5mmol of 2-acetamido-4-methyl aminobenzoate and 5mmol of 2-amino-3, 5-dibromobenzaldehyde into a reaction vessel according to the proportion, adding 800mL of ethanol and 10mL of formic acid, heating to reflux, reacting for 8h, cooling to room temperature, performing suction filtration, and washing with ethanol to obtain a bright yellow solid; dissolving the bright yellow solid in 300mL of methanol and 500mL of 5M NaOH, reacting for 30h at 90 ℃, detecting complete reaction by TCL, recovering the solvent to 400mL under reduced pressure, adjusting the pH value to 6, performing suction filtration, and recrystallizing the filter residue with ethanol to obtain a white solid; putting the white solid of the product in 150mL of acetonitrile, adding 36mmol of NaH at 0 ℃, stirring for 30min, adding 36mmol of allyl chloroformate, reacting for 4-5h at 60 ℃, detecting complete reaction by TCL, adding 200mL of water, extracting for three times by using 100mL of ethyl acetate, washing for three times by using 100mL of saturated sodium bicarbonate and 100mL of saturated saline solution respectively, collecting an organic phase, and pulping by using ethyl acetate and petroleum ether to obtain the imprinted template molecule.
3. Use of the imprinted template molecule of claim 1 for the preparation of molecularly imprinted polymers, characterized by the steps of:
a. 1.0g of surface amino group-modified Fe as a core material3O4@SiO22.0g of imprinting template molecules of the formula (I) are dispersed in 50mL of toluene-acetonitrile, wherein the volume ratio of toluene is 0-50%, and the mixture is stirred at room temperature for 1h for prepolymerization; adsorbing the solution at the bottom of a bottle by using a magnet, pouring out supernatant, and adding 50mL of toluene-acetonitrile, wherein the volume ratio of toluene is 0-50%, so as to obtain a pre-polymerization solution;
b. adding 6.0g of crosslinking agent ethylene glycol dimethacrylate and 0.3g of initiator azobisisobutyronitrile into the prepolymerization liquid, introducing nitrogen to remove oxygen, sealing, and carrying out polymerization reaction in a heating and stirring manner to generate powdery polymer MIPs, wherein the polymerization reaction temperature is 55 ℃ and the reaction time is 36 hours;
c. and (3) repeatedly carrying out ultrasonic elution on the obtained MIPs by using 1moL/L hydrochloric acid-methanol solution to remove template molecules, then washing the MIPs to be neutral by using methanol, and drying the polymer in a vacuum drying oven until the weight is balanced to obtain the molecularly imprinted polymer MIPs with the core-shell structure.
4. The use of the imprinted template molecule of claim 1 in PSD-95/nNOS protein interaction inhibitor high throughput rapid screening.
5. The use of the imprinted template molecule of claim 1 in the preparation of a PSD-95/nNOS protein interaction inhibitor high-throughput rapid screening kit.
6. The use of molecularly imprinted polymer MIPs prepared according to the method of claim 3 for preparing a PSD-95/nNOS protein interaction inhibitor high-throughput rapid screening kit.
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565485A (en) * | 2009-05-22 | 2009-10-28 | 南京医科大学 | Method for preparing molecularly imprinted polymers of ethinylestradiol analogue |
| CN101817908A (en) * | 2010-02-04 | 2010-09-01 | 南京医科大学 | Preparation method of molecular imprinting polymer of PSD-95/nNOS uncoupler |
| CN107353372A (en) * | 2017-06-21 | 2017-11-17 | 南京医科大学 | A kind of preparation method of the uncoupler imprint surface polymer of nNOS PSD 95 based on magnetic mesoporous molecular sieve |
Non-Patent Citations (2)
| Title |
|---|
| "A surface magnetic imprinted polymers as artificial receptors for selective and efficient capturing of new neuronal nitric oxide synthase–post synaptic density protein-95 uncouplers";Yao Dandan等;《Journal of Pharmaceutical and Biomedical Analysis》;20180303;第154卷;第180-190页 * |
| "Novel surface imprinted magnetic mesoporous silica as artificial antibodies for efficient discovery and capture of candidate nNOS–PSD-95 uncouplers for stroke treatment";Huang Jiaojiao等;《J. Mater. Chem. B》;20180206;第6卷;第1531-1542页 * |
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