CN115844023A - Sea cucumber peptidase sea buckthorn powder and preparation method thereof - Google Patents
Sea cucumber peptidase sea buckthorn powder and preparation method thereof Download PDFInfo
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Abstract
The invention discloses 100 parts of sea cucumber, 25 parts of ionized water, 15 parts of protease, 20 parts of sea buckthorn powder, 10 parts of dandelion extract and 4 parts of trace elements. The sea cucumber polypeptide is a sea cucumber extract, has high nutritive value, can repair damaged cells after entering a human body, enhances the immunity of the human body, can play the effects of resisting fatigue and aging, and can regulate the human body after daily administration. The sea cucumber polypeptide is suitable for tumor patients, sub-health people and brainworkers.
Description
Technical Field
The invention relates to the technical field of health products, in particular to sea cucumber peptidase sea buckthorn powder and a preparation method thereof.
Background
Hypertension, hyperglycemia and hyperlipidemia are indicated. The three highs have great harm to human bodies, can accelerate the development of arteriosclerosis, and can cause cardiovascular and cerebrovascular diseases such as coronary heart disease, angina pectoris, even acute myocardial infarction, cerebral apoplexy and the like if the control is not good for a long time. Pancreatitis and fatty liver are also easily caused by hyperlipidemia.
Since blood sugar control is unstable and diabetic nephropathy is often induced, the harm of hypertension, hyperglycemia and hyperlipidemia to human bodies is great. In the ordinary days, people need to improve diet and living habits, need to work and rest on a low-salt and low-fat diet rule, eat fresh fruits and vegetables, and exercise to lose weight. Patients with abnormal indexes need to be actively treated, especially the old patients can be preventively orally taken statin lipid-lowering drugs such as atorvastatin and rosuvastatin for a long time, so that the blood fat can be reduced, and the progress of arteriosclerosis can be prevented.
Hypertension itself can accelerate the development of arteriosclerosis, and anti-platelet drugs such as aspirin can be used in combination with antihypertensive drugs. Patients with abnormal blood sugar, for patients with diabetes, must avoid diet, take oral hypoglycemic drugs actively, or inject insulin subcutaneously, so as to reduce or prevent the occurrence of complications.
Disclosure of Invention
The invention provides sea cucumber peptidase sea buckthorn powder and a preparation method thereof in order to overcome the defects in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme: the sea cucumber peptidase sea buckthorn powder comprises the following components in parts by weight: 100-120 parts of sea cucumber, 25-35 parts of ionized water, 15-19 parts of protease, 20-30 parts of sea buckthorn powder, 10-15 parts of dandelion extract and 4-7 parts of trace elements.
Preferably, the preparation method of the sea cucumber peptidase sea buckthorn powder comprises the following steps: s1, soaking and washing sea cucumbers in salt water, and chopping the sea cucumbers after washing to obtain sea cucumber meat;
s2, pouring protease into the minced sea cucumber meat, stirring, carrying out enzymolysis for a period of time, sealing, and concentrating and removing impurities to obtain a sea cucumber polypeptide solution;
s3, slowly dripping the dandelion extract into the sea cucumber polypeptide solution while stirring, and adding the trace elements after stirring to obtain a sea cucumber polypeptide drink;
and S4, adding the sea buckthorn powder into the sea cucumber polypeptide drink, slowly stirring, and freeze-drying to obtain powdery sea cucumber peptidase sea buckthorn powder after stirring.
Preferably, in the step S2, after the ph value of the sea cucumber meat is adjusted to a protease adaptation value, 70% of total protease is poured in, stirring is performed for the first enzymolysis, stirring is performed for 5-10 minutes, then sealing is performed for 1-2 hours, after the first enzymolysis, freezing and centrifuging are performed to obtain a sea cucumber polypeptide zymolyte, ionized water is poured in the sea cucumber polypeptide zymolyte for mixing, the temperature is increased to 35-45 ℃, after standing, the remaining 30% of total protease is poured in for the second enzymolysis, the time for the second enzymolysis is 1.5-2 hours, after the second enzymolysis, enzyme is inactivated at high temperature, and filtration is performed to obtain a sea cucumber polypeptide solution.
Preferably, in the step S2, after the ph value of the sea cucumber meat is adjusted to a protease adaptation value, 75% of total protease is poured in, stirring is performed for the first enzymolysis, stirring is performed for 5-8 minutes, then sealing is performed for 1-1.5 hours, after the first enzymolysis, freezing and centrifuging are performed to obtain a sea cucumber polypeptide zymolyte, ionized water is poured in the sea cucumber polypeptide zymolyte for mixing, the temperature is increased to 37-41 ℃, standing is performed, then the remaining 25% of total protease is poured in for the second enzymolysis, the time of the second enzymolysis is 1.5-1.7 hours, after the second enzymolysis, enzyme is inactivated at high temperature, sealing is performed, and then concentration and impurity removal are performed to obtain a sea cucumber polypeptide solution, which is a final product.
Preferably, the preparation method of the dandelion extract comprises the following steps: washing and drying dandelion, crushing to obtain dandelion powder, adding 2-4 times of water into the dandelion powder, mixing, adding a compound enzyme for enzymolysis, wherein the total amount of the compound enzyme is 0.25-0.30% of the total amount of the dandelion, and sequentially performing a concentration extraction process after enzymolysis to obtain the dandelion extract.
Preferably, in the preparation method of the dandelion extract, the complex enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 1.5-2h, and the enzymolysis temperature is 30-40 ℃.
Preferably, the preparation method of the dandelion extract comprises the following steps: washing and drying dandelion, crushing to obtain dandelion powder, adding 2.5 times of water into the dandelion powder, mixing, adding a complex enzyme for enzymolysis, wherein the total amount of the complex enzyme is 0.27% of the total amount of the dandelion, the complex enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 18h, and the dandelion extract is obtained by sequentially passing through a concentration extraction process after enzymolysis at the enzymolysis temperature of 37 ℃.
Compared with the prior art, the invention has the following effects: the sea cucumber polypeptide is a sea cucumber extract, has high nutritive value, can repair damaged cells after entering a human body, enhances the immunity of the human body, simultaneously has the effects of resisting fatigue and aging, and can regulate the human body after daily administration. The sea cucumber polypeptide is suitable for tumor patients, sub-health people and brainworkers.
The sea cucumber polypeptide has a certain regulating effect on blood pressure, blood fat and blood sugar, can be used for regulating blood sugar, blood fat and blood pressure in daily life, and is suitable for patients with diabetes, hyperlipidemia and hypertension. Each patient has different constitutions and should be used under the guidance of a doctor.
Fructus Hippophae powder has effects of reducing cholesterol content, and reducing blood lipid, and can be used for preventing and treating cardiovascular disease and cerebrovascular disease such as atherosclerosis, myocardial infarction, cerebral embolism, hyperlipidemia, etc.; the fructus Hippophae powder contains abundant oleum Hippophae, and has effects of relieving fatigue and enhancing immunity, thereby preventing diseases and delaying aging.
The dandelion tea has the effects of clearing away heat and toxic materials, promoting urination and resolving masses, and patients with high blood pressure, high blood fat and high blood sugar are usually accompanied by vigorous liver fire and yellow urine, and the dandelion tea can clear away heat and toxic materials while reducing the high blood pressure, high blood fat and high blood fat.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
The sea cucumber peptidase sea buckthorn powder comprises the following components in parts by weight: 100 parts of sea cucumber, 25 parts of ionized water, 15 parts of protease, 20 parts of sea buckthorn powder, 10 parts of dandelion extract and 4 parts of trace elements.
Example 2
The sea cucumber peptidase sea buckthorn powder comprises the following components in parts by weight: 114 parts of sea cucumber, 29 parts of ionized water, 16 parts of protease, 22 parts of sea buckthorn powder, 13 parts of dandelion extract and 5 parts of trace elements.
Example 3
The sea cucumber peptidase sea buckthorn powder comprises the following components in parts by weight: 120 parts of sea cucumber, 35 parts of ionized water, 19 parts of protease, 30 parts of sea buckthorn powder, 15 parts of dandelion extract and 7 parts of trace elements.
Comparative example 1
The comparative example was similar to example 2 except that no dandelion extract was added, as compared with example 2.
Comparative example 2
This comparative example was similar to example 2 except that no trace elements were added, as compared with example 2.
Sea cucumber peptidase sea buckthorn powder is prepared according to the same preparation method of the examples 1-3 and the comparative examples 1-2;
selecting 120 male mice with weight of 18-22g and good health condition, and averagely dividing into 7 groups, wherein 5 groups are administration groups, and the other two groups are a blank group and a model group respectively; each group of 10 mice is fed with high-calorie food to the mice in the administration group and the model group, so as to form a hypertension, hyperlipidemia and hyperglycemia model; respectively performing intragastric administration on the sea cucumber peptidase sea buckthorn powder in each example and each comparative example to mice in an administration group, wherein the administration amount is 0.4mL, the administration is performed once a day, and the administration is continued for 15 days; the blank group and the model group were given an equal amount of physiological saline, 0.4mL once a day, for 15 days; respectively carrying out tail blood sampling on the mice on days 1, 3 and 7 after the model building, recording the physical sign characteristics of each group of mice, testing blood sugar, blood fat and blood pressure, and averaging each group;
the blood pressure, blood glucose and blood lipid levels of the mice in each group are shown in Table 1.
TABLE 1
As shown in table 1, example 2 is the most preferable embodiment, and the blood pressure-lowering and blood glucose-lowering effects on mice in example 2 are the best as compared with comparative examples 1-2, example 1 and example 3.
Example 4
A preparation method of sea cucumber peptidase sea buckthorn powder comprises the following steps: s1, soaking and washing sea cucumbers in salt water, and chopping the sea cucumbers after washing to obtain sea cucumber meat;
s2, adjusting the pH value of the minced sea cucumber meat to a protease adaptation value, then pouring 70% of protease of the total amount, stirring for carrying out first enzymolysis, stirring for 5 minutes, sealing for 1 hour, after the first enzymolysis, freezing and centrifuging to obtain a sea cucumber polypeptide zymolyte, pouring ionized water into the sea cucumber polypeptide zymolyte for mixing, heating to 35 ℃, standing, then pouring the remaining 30% of protease of the total amount for carrying out second enzymolysis, wherein the second enzymolysis time is 1.5 hours, sealing after the second enzymolysis, carrying out high-temperature enzyme inactivation after sealing, and carrying out concentration, impurity removal and filtration to obtain a sea cucumber polypeptide solution;
s3, slowly dripping the dandelion extract into the sea cucumber polypeptide solution while stirring, and adding the trace elements after stirring to obtain the sea cucumber polypeptide drink;
and S4, adding the sea buckthorn powder into the sea cucumber polypeptide drink, slowly stirring, and freeze-drying to obtain powdery sea cucumber peptidase sea buckthorn powder after stirring.
The preparation method of the dandelion extract comprises the following steps: washing and drying dandelion, crushing to obtain dandelion powder, adding 2 times of water into the dandelion powder, mixing, adding a compound enzyme for enzymolysis, wherein the total amount of the compound enzyme is 0.25% of the total amount of the dandelion, and sequentially performing a concentration extraction process after enzymolysis to obtain a dandelion extract, wherein in the preparation method of the dandelion extract, the compound enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 1.5h, and the enzymolysis temperature is 30 ℃.
Example 5
A preparation method of sea cucumber peptidase sea buckthorn powder comprises the following steps: s1, soaking and washing sea cucumbers in salt water, and chopping the sea cucumbers after washing to obtain sea cucumber meat;
s2, adjusting the pH value of the minced sea cucumber meat to a protease adaptation value, then pouring 70% of protease of the total amount, stirring for carrying out first enzymolysis, stirring for 6 minutes, sealing for 1.5 hours, after the first enzymolysis, freezing and centrifuging to obtain a sea cucumber polypeptide zymolyte, pouring ionized water into the sea cucumber polypeptide zymolyte, mixing, heating to 37 ℃, standing, then pouring the remaining 30% of protease of the total amount for carrying out second enzymolysis, wherein the second enzymolysis time is 1.5 hours, sealing after the second enzymolysis, carrying out high-temperature enzyme killing after sealing, and concentrating, removing impurities and filtering to obtain a sea cucumber polypeptide solution;
s3, slowly dripping the dandelion extract into the sea cucumber polypeptide solution while stirring, and adding the trace elements after stirring to obtain the sea cucumber polypeptide drink;
and S4, adding the sea buckthorn powder into the sea cucumber polypeptide drink, slowly stirring, and freeze-drying to obtain powdery sea cucumber peptidase sea buckthorn powder after stirring.
The preparation method of the dandelion extract comprises the following steps: washing and drying dandelion, crushing to obtain dandelion powder, adding 2.5 times of water into the dandelion powder, mixing, adding a compound enzyme for enzymolysis, wherein the total amount of the compound enzyme is 0.27% of the total amount of the dandelion, sequentially performing a concentration extraction process after enzymolysis to obtain a dandelion extract, wherein in the preparation method of the dandelion extract, the compound enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 1.8h, and the enzymolysis temperature is 37 ℃.
Example 6
A preparation method of sea cucumber peptidase sea buckthorn powder comprises the following steps: s1, soaking and washing sea cucumbers in salt water, and chopping the sea cucumbers after washing to obtain sea cucumber meat;
s2, adjusting the pH value of the minced sea cucumber meat to a protease adaptation value, then pouring 70% of protease of the total amount, stirring for carrying out first enzymolysis, stirring for 10 minutes, sealing for 1-2 hours, after the first enzymolysis, freezing and centrifuging to obtain a sea cucumber polypeptide zymolyte, pouring ionized water into the sea cucumber polypeptide zymolyte for mixing, heating to 45 ℃, standing, then pouring the remaining 30% of protease of the total amount for carrying out second enzymolysis, wherein the second enzymolysis time is 2 hours, sealing after the second enzymolysis, carrying out high-temperature enzyme inactivation after sealing, and carrying out concentration, impurity removal and filtration to obtain a sea cucumber polypeptide solution;
s3, slowly dripping the dandelion extract into the sea cucumber polypeptide solution while stirring, and adding the trace elements after stirring to obtain the sea cucumber polypeptide drink;
and S4, adding the sea buckthorn powder into the sea cucumber polypeptide drink, slowly stirring, and freeze-drying to obtain powdery sea cucumber peptidase sea buckthorn powder after stirring.
The preparation method of the dandelion extract comprises the following steps: washing and drying dandelion, crushing to obtain dandelion powder, adding 4 times of water into the dandelion powder, mixing, adding a complex enzyme for enzymolysis, wherein the total amount of the complex enzyme is 0.30% of the total amount of the dandelion, and sequentially performing a concentration extraction process after enzymolysis to obtain a dandelion extract, wherein in the preparation method of the dandelion extract, the complex enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 2 hours, and the enzymolysis temperature is 40 ℃.
Comparative example 3
In comparison with example 5, in comparative example 3, the protease was not added twice for the enzymatic hydrolysis, and the rest was the same as example 5.
Comparative example 4
In comparison with example 5, in the preparation of dandelion extract, the comparative example 4 did not add complex enzyme to dandelion for enzymolysis, and the rest was the same as example 5.
Comparative example 5
In comparison with example 5, in the preparation of dandelion extract, the complex enzyme of control example 5 was not added with cellulase and amylase, and the rest was the same as example 5.
Selecting 140 male mice with weight of 18-22g and good health condition, and averagely dividing into 8 groups, wherein 6 groups are administration groups, and the other two groups are blank group and model group respectively; each group contains 10 mice, and the mice in the administration group and the model group eat high-calorie food to cause a hypertension, hyperlipidemia and hyperglycemia model; respectively performing intragastric administration on the sea cucumber peptidase sea buckthorn powder in each embodiment and each proportion to mice in an administration group, wherein the administration amount is 0.4mL, the administration is performed once a day, and the administration is continued for 15 days; the blank group and the model group were given an equal amount of physiological saline, 0.4mL once a day, for 15 days; respectively carrying out tail blood sampling on the mice on days 1, 3 and 7 after the model building, recording the physical sign characteristics of each group of mice, testing blood sugar, blood fat and blood pressure, and averaging each group;
the blood pressure, blood glucose and blood lipid levels of the mice in each group are shown in Table 2.
TABLE 2
As shown in table 2, example 5 is the most preferable embodiment, and the effect of reducing three highs was the best in example 2 compared with comparative examples 3 to 5, example 4 and example 6.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the patent and protection scope of the present invention should be subject to the appended claims.
Claims (7)
1. The sea cucumber peptidase sea buckthorn powder is characterized by comprising the following components in parts by weight: 100-120 parts of sea cucumber, 25-35 parts of ionized water, 15-19 parts of protease, 20-30 parts of sea buckthorn powder, 10-15 parts of dandelion extract and 4-7 parts of trace elements.
2. The method for preparing sea cucumber peptidase sea buckthorn powder according to claim 1, comprising the steps of:
s1, soaking and washing sea cucumbers in salt water, and chopping the sea cucumbers after washing to obtain sea cucumber meat;
s2, pouring protease into the minced sea cucumber meat, stirring, carrying out enzymolysis for a period of time, sealing, and concentrating and removing impurities to obtain a sea cucumber polypeptide solution;
s3, slowly dripping the dandelion extract into the sea cucumber polypeptide solution while stirring, and adding the trace elements after stirring to obtain the sea cucumber polypeptide drink;
and S4, adding the sea buckthorn powder into the sea cucumber polypeptide drink, slowly stirring, and freeze-drying to obtain powdery sea cucumber peptidase sea buckthorn powder after stirring.
3. The preparation method of sea cucumber peptidase sea buckthorn powder according to claim 2, wherein in the step S2, after the ph value of sea cucumber meat is adjusted to a protease adaptation value, 70% of total protease is poured in, the mixture is stirred for first enzymolysis, the mixture is stirred for 5-10 minutes and then sealed for 1-2 hours, after the first enzymolysis, the mixture is frozen and centrifuged to obtain sea cucumber polypeptide zymolyte, ionized water is poured in the sea cucumber polypeptide zymolyte for mixing, the temperature is increased to 35-45 ℃, the mixture is kept stand and then poured in the remaining 30% of total protease for second enzymolysis, the time of the second enzymolysis is 1.5-2 hours, and after the second enzymolysis, enzyme is inactivated at high temperature, and the sea cucumber polypeptide solution is obtained by filtration.
4. The preparation method of the sea cucumber peptidase sea buckthorn powder of claim 2, wherein in the step S2, after the pH value of sea cucumber meat is adjusted to a protease adaptation value, 75% of total protease is poured into the sea cucumber meat, stirring is carried out for the first enzymolysis, 5-8 minutes of stirring is carried out, then sealing is carried out for 1-1.5 hours, after the first enzymolysis, freezing and centrifuging are carried out, a sea cucumber polypeptide zymolyte is obtained, ionized water is poured into the sea cucumber polypeptide zymolyte for mixing, the temperature is increased to 37-41 ℃, after standing, the remaining 25% of total protease is poured into the sea cucumber polypeptide zymolyte for the second enzymolysis, the second enzymolysis time is 1.5-1.7 hours, after the second enzymolysis, enzyme is killed at a high temperature, after sealing, concentration and impurity removal are carried out, so that a sea cucumber polypeptide solution is obtained, and the sea cucumber polypeptide solution is a final product.
5. The method for preparing sea cucumber peptidase sea buckthorn powder according to claim 3 or 4, wherein the method for preparing the dandelion extract comprises: washing and drying dandelion, crushing to obtain dandelion powder, adding 2-4 times of water into the dandelion powder, mixing, adding a compound enzyme for enzymolysis, wherein the total amount of the compound enzyme is 0.25-0.30% of the total amount of the dandelion, and sequentially performing a concentration extraction process after enzymolysis to obtain the dandelion extract.
6. The method for preparing sea cucumber peptidase sea buckthorn powder according to claim 5, wherein in the method for preparing the dandelion extract, the complex enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 1.5-2h, and the enzymolysis temperature is 30-40 ℃.
7. The method for preparing sea cucumber peptidase sea buckthorn powder according to claim 3 or 4, wherein the method for preparing the dandelion extract comprises: washing and drying dandelion, crushing to obtain dandelion powder, adding 2.5 times of water into the dandelion powder, mixing, adding a complex enzyme for enzymolysis, wherein the total amount of the complex enzyme is 0.27% of the total amount of the dandelion, the complex enzyme comprises cellulase, pectinase and amylase, the enzymolysis time is 18h, and the dandelion extract is obtained by sequentially passing through a concentration extraction process after enzymolysis at the enzymolysis temperature of 37 ℃.
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