CN115825026A - Automatic aerosol pathogen monitoring device, system and monitoring method - Google Patents
Automatic aerosol pathogen monitoring device, system and monitoring method Download PDFInfo
- Publication number
- CN115825026A CN115825026A CN202211460504.2A CN202211460504A CN115825026A CN 115825026 A CN115825026 A CN 115825026A CN 202211460504 A CN202211460504 A CN 202211460504A CN 115825026 A CN115825026 A CN 115825026A
- Authority
- CN
- China
- Prior art keywords
- sample
- module
- chip
- aerosol
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000443 aerosol Substances 0.000 title claims abstract description 109
- 244000052769 pathogen Species 0.000 title claims abstract description 65
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 56
- 238000012806 monitoring device Methods 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000012544 monitoring process Methods 0.000 title claims description 21
- 238000001514 detection method Methods 0.000 claims abstract description 108
- 238000002347 injection Methods 0.000 claims abstract description 71
- 239000007924 injection Substances 0.000 claims abstract description 71
- 239000012530 fluid Substances 0.000 claims abstract description 59
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 45
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 45
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 45
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 38
- 230000003321 amplification Effects 0.000 claims abstract description 32
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims description 67
- 238000004140 cleaning Methods 0.000 claims description 26
- 238000010438 heat treatment Methods 0.000 claims description 24
- 230000002572 peristaltic effect Effects 0.000 claims description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 230000014759 maintenance of location Effects 0.000 claims description 17
- 239000002699 waste material Substances 0.000 claims description 13
- 230000017525 heat dissipation Effects 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 238000012856 packing Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 239000007791 liquid phase Substances 0.000 claims description 4
- 238000013102 re-test Methods 0.000 claims description 4
- 230000001351 cycling effect Effects 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 description 170
- 238000005070 sampling Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 244000000010 microbial pathogen Species 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000711573 Coronaviridae Species 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241000713196 Influenza B virus Species 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 241000725643 Respiratory syncytial virus Species 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 208000037798 influenza B Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000013615 primer Substances 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Abstract
The invention provides an automatic aerosol pathogen monitoring device, system and method, relating to the technical field of biological detection, wherein the automatic aerosol pathogen monitoring device comprises: the collection module comprises an aerosol collection device; a chip module comprising at least one microfluidic chip; the sample injection module comprises a sample injection pipeline, and the sample injection pipeline is provided with a sample injection state which is communicated with the aerosol acquisition device and the microfluidic chip; the detection module comprises a fluid driving mechanism, a nucleic acid amplification mechanism and a fluorescence detection mechanism; and the control module is electrically connected with the acquisition module, the chip module, the sample injection module and the detection module, and can control the sample injection state, the driving state, the amplification state and the scanning state. The invention can still automatically complete the detection operation of aerosol pathogens according to the setting under the unattended condition, thereby greatly improving the detection efficiency of the aerosol pathogens.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to an automatic aerosol pathogen monitoring device, system and method.
Background
Bioaerosols refer to fine solid or liquid particles suspended in air that contain associated pathogenic microorganisms, such as bacteria, fungi, viruses, and the like. Microorganisms are able to survive in air for hours or days and can be transmitted by means of air jets.
In the prior art, pathogenic microorganisms in aerosol are mainly detected by adopting etiology based on colony counting, visible microbial colonies can be obtained only after a plurality of days when the detection method needs to culture the microorganisms collected in the aerosol in a constant-temperature incubator, and the detection result of the pathogenic microorganisms in the aerosol is obtained by analyzing the visible microbial colonies through manual statistical operation. However, the detection method has the problems of long detection period, poor quantitative accuracy, complicated detection process and the like, and the detection process of pathogens in the aerosol sample cannot be efficiently realized.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, embodiments of the present invention provide an automatic aerosol pathogen monitoring device, system and monitoring method, which are used to automatically complete the sampling and detection operations of aerosol.
The above object of the present invention can be achieved by the following technical solutions, and the present invention provides an automatic aerosol pathogen monitoring device, including: an acquisition module comprising an aerosol acquisition device; a chip module comprising at least one microfluidic chip; the sample injection module comprises a sample injection pipeline, and the sample injection pipeline has a sample injection state which is communicated with the aerosol acquisition device and the microfluidic chip; the detection module comprises a fluid driving mechanism, a nucleic acid amplification mechanism and a fluorescence detection mechanism, wherein the fluid driving mechanism has a driving state for driving the fluid in the microfluidic chip to flow, the nucleic acid amplification mechanism has an amplification state for amplifying the nucleic acid in the microfluidic chip, and the fluorescence detection mechanism has a scanning state for detecting a fluorescence signal of the nucleic acid in the microfluidic chip; and the control module is electrically connected with the acquisition module, the chip module, the sample injection module and the detection module, and can control the sample injection state, the driving state, the amplification state and the scanning state.
In a preferred embodiment of the present invention, the aerosol collecting device includes a cyclone separator and a sample container disposed at a liquid phase outlet of the cyclone separator, and the sample container is controllably communicated with the microfluidic chip through the sample injection pipeline.
In a preferred embodiment of the present invention, the chip module further includes a driving mechanism and a plurality of chip trays disposed on the driving mechanism, the plurality of microfluidic chips are disposed on the chip tray at intervals, and the driving mechanism can drive the chip tray to move to drive at least one of the microfluidic chips to enter the sample injection station.
In a preferred embodiment of the present invention, a sample feeding peristaltic pump is disposed on the sample feeding pipeline, one end of the sample feeding pipeline is communicated with the sample container, and a sample feeding hollow needle is disposed at the other end of the sample feeding pipeline, and in the sample feeding state, the sample feeding hollow needle is communicated with the microfluidic chip.
In a preferred embodiment of the present invention, a first one-way valve, the sample peristaltic pump, the debubbling device and a flow meter are sequentially disposed on the sample injection pipeline along a direction from the sample container to the sample injection hollow needle.
In a preferred embodiment of the present invention, the sample introduction module further includes a collection liquid pipeline, one end of the collection liquid pipeline is communicated with the sample container, the other end of the collection liquid pipeline is communicated with the collection liquid container, and a second one-way valve and a liquid inlet peristaltic pump are sequentially disposed on the collection liquid pipeline along a direction from the sample container to the collection liquid container.
In a preferred embodiment of the present invention, the sample injection module further includes a sample holding tube, one end of the sample holding tube is provided with a sample holding container, a first two-way valve is disposed on the sample injection tube between the sample injection peristaltic pump and the bubble remover, and the other end of the sample holding tube is connected to the first two-way valve.
In a preferred embodiment of the present invention, the sample injection module further includes a waste liquid container, one end of the sample retaining pipe is provided with a second two-way valve, and the waste liquid container and the sample retaining pipe are arranged in parallel at one end of the sample retaining pipe through the second two-way valve.
In a preferred embodiment of the present invention, the sample injection module further includes a cleaning solution container, one end of the collection liquid pipeline is provided with a third two-way valve, and the cleaning solution container and the collection liquid container are arranged in parallel at one end of the collection liquid pipeline through the third two-way valve.
In a preferred embodiment of the present invention, the microfluidic chip comprises: a chip housing; a sample tube unit, a washing tube unit and a reagent tube unit which are arranged on the chip shell; the chip upper cover and the chip lower piece are oppositely arranged at two ends of the chip shell, the chip lower piece is provided with a fluid pipeline, and the fluid pipeline is communicated with the sample tube unit, the cleaning tube unit and the reagent tube unit; and a detection area arranged on the lower part of the chip, wherein the detection area is provided with nucleic acid capture filter paper.
In a preferred embodiment of the present invention, the nucleic acid capture filter paper comprises a chitosan-modified filter disposed at the detection region for nucleic acid capture.
In a preferred embodiment of the present invention, the sample tube unit includes a sample tube disposed on the chip housing, and an inlet of the sample tube is provided with a rubber plug and an annular packing paper covering the rubber plug.
In a preferred embodiment of the present invention, the fluid driving mechanism includes a fluid driving ejector rod and a lifting driving motor for driving the fluid driving ejector rod to lift, the sample injection station is disposed below the fluid driving ejector rod, and in the driving state, the fluid driving ejector rod can be pressed against the sample tube unit for fluid driving.
In a preferred embodiment of the present invention, the detection module further includes a chamber selection motor, the chamber selection motor is connected to the fluid driving mechanism through a connecting member, and the chamber selection motor can push the fluid driving mechanism to move, so as to drive the fluid driving ejector rod to controllably press and connect with one of the sample tube unit, the wash tube unit and the reagent tube unit.
In a preferred embodiment of the present invention, the nucleic acid amplification mechanism includes a heating structure and a heat dissipation structure disposed on the heating structure, and in the amplification state, the heating structure is capable of performing temperature cycling on the microfluidic chip located at the sample injection station.
In a preferred embodiment of the present invention, the heating structure includes a heating block, and the heat dissipation structure includes a water-cooled heat dissipation block disposed on the heating block.
In a preferred embodiment of the present invention, the fluorescence detection mechanism includes a four-color fluorescence detection unit and a fluorescence scanning motor for driving the four-color fluorescence detection unit, and in the scanning state, the four-color fluorescence detection unit can perform fluorescence detection processing on the microfluidic chip located at the sample injection station.
In a preferred embodiment of the present invention, the control module includes a main control unit, and a first sub-control unit and a second sub-control unit electrically connected to the main control unit, wherein the first sub-control unit is electrically connected to the acquisition module, the chip module and the sample injection module, and the second sub-control unit is electrically connected to the detection module.
The invention also provides an automatic aerosol pathogen monitoring system which comprises at least one automatic aerosol pathogen monitoring device and an antenna network module, wherein the detection module is electrically connected with the antenna network module, and the antenna network module has a monitoring state for acquiring a detection result of the detection module.
The invention also provides a monitoring method applying the automatic aerosol pathogen monitoring system, which comprises the following steps:
starting an acquisition module to collect aerosol samples in the air;
injecting the aerosol sample into a microfluidic chip of a chip module by using a sample injection pipeline of a sample injection module;
carrying out temperature cycle processing and fluorescence detection processing on the microfluidic chip through a detection module;
acquiring a detection result of the detection module and uploading the detection result to an skynet module;
when a pathogen is detected, the detection result is positive, and the micro-fluidic chip is replaced with a new micro-fluidic chip for rechecking;
when the recheck structure is negative, the skynet module responds to a primary alarm;
the skynet module responds to a high-level alarm when the retest structure is positive.
In a preferred embodiment of the present invention, before the collection module is activated by the control module to collect the aerosol sample in the air, the method further includes the following steps:
and sending a reset instruction through the control module, and resetting the automatic aerosol pathogen monitoring device and the skynet module to an initial state.
In a preferred embodiment of the present invention, the acquiring, by the skynet module, the detection result of the detection module further includes the following steps:
when the detection result is negative, the detection module finishes the detection;
collecting waste liquid of the aerosol sample left in the acquisition module;
and cleaning the sample injection pipeline for next detection.
The technical scheme of the invention has the following remarkable beneficial effects:
when the automatic aerosol pathogen monitoring device is used, the acquisition module is arranged in a target area, and the control module controls the aerosol acquisition device to acquire an aerosol sample in the target area. The collected aerosol sample is delivered into a micro-fluidic chip of a chip module through a sample injection module, fluid in the micro-fluidic chip can be driven to flow through a fluid driving mechanism of a detection module, so that the micro-fluidic chip can be used for performing operations such as virus splitting, nucleic acid capturing, nucleic acid cleaning and the like on the aerosol sample, a nucleic acid amplification effect can be realized by matching a nucleic acid amplification mechanism of the detection module with the micro-fluidic chip, and finally, the fluorescence detection mechanism of the detection module can be used for performing fluorescence detection on the processed aerosol sample, so that the detection result of pathogens in the aerosol can be quickly obtained.
The invention can automatically complete the detection process by utilizing the matching of the control module and other modules, and has the advantages of high integration level, high detection efficiency and more accurate detection result. And the invention combines the micro-fluidic chip and the fluorescence detection mechanism, and has the advantages of high sensitivity, high specificity and capability of realizing multi-index nucleic acid amplification detection.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
The drawings described herein are for illustration purposes only and are not intended to limit the scope of the present disclosure in any way. In addition, the shapes, the proportional sizes, and the like of the respective members in the drawings are merely schematic for facilitating the understanding of the present invention, and do not specifically limit the shapes, the proportional sizes, and the like of the respective members of the present invention. Those skilled in the art, having the benefit of the teachings of this invention, may choose from the various possible shapes and proportional sizes to implement the invention as a matter of case.
FIG. 1 is a schematic diagram of an automated aerosol pathogen monitoring system;
FIG. 2 is a schematic diagram of a sample injection module;
FIG. 3 is a schematic structural diagram of a detection module;
FIG. 4 is a schematic diagram of a chip module;
FIG. 5 is a schematic diagram of an exploded structure of the microfluidic chip;
fig. 6 is a schematic sectional view of the sample tube unit.
Reference numerals of the above figures:
1. an acquisition module; 11. an aerosol collection device; 111. a cyclone separator; 112. a sample container;
2. a chip module; 21. a microfluidic chip; 211. a chip housing; 212. a sample tube unit; 2121. a sample tube; 2122. a rubber plug; 2123. annular packing paper; 213. a cleaning pipe unit; 214. a reagent tube unit; 215. an upper chip cover; 216. chip unloading; 217. detecting a region; 22. a drive mechanism; 221. a tray slide rail; 222. the chip drives the electrical machinery; 23. a chip tray;
3. a sample introduction module; 31. a sample introduction pipeline; 311. a sample feeding peristaltic pump; 312. a first one-way valve; 313. a bubble remover; 314. a flow meter; 315. a sample injection hollow needle; 32. a collection liquid pipeline; 321. a collection liquid container; 322. a second one-way valve; 323. a liquid inlet peristaltic pump; 33. a sample retention conduit; 331. a sample retention container; 332. a first two-way valve; 34. a waste liquid container; 341. a second two-way valve; 35. a cleaning solution container; 351. a third two-way valve;
4. a detection module; 41. a fluid drive mechanism; 411. the fluid drives the ejector pin; 412. a lifting drive motor; 413. a chamber selection motor; 42. a nucleic acid amplification mechanism; 421. a heating structure; 422. a heat dissipation structure; 43. a fluorescence detection mechanism; 431. a four-color fluorescence detection unit; 432. a fluorescent scanning motor;
5. a control module; 51. a first sub-control unit; 52. a second sub-control unit;
6. and an skynet module.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Implementation mode one
Referring to fig. 1, in one embodiment of the present invention, an automatic aerosol pathogen monitoring device is provided, comprising: the system comprises an acquisition module 1, wherein the acquisition module 1 comprises an aerosol acquisition device 11; a chip module 2, the chip module 2 comprising at least one microfluidic chip 21; the sample injection module 3, the sample injection module 3 includes the sample injection pipeline 31, the sample injection pipeline 31 has the sample injection state communicating the aerosol collecting device 11 and the microfluidic chip 21; the detection module 4, the detection module 4 includes a fluid driving mechanism 41, a nucleic acid amplification mechanism 42 and a fluorescence detection mechanism 43, the fluid driving mechanism 41 has a driving state for driving the fluid in the microfluidic chip 21 to flow, the nucleic acid amplification mechanism 42 has an amplification state for amplifying the nucleic acid in the microfluidic chip 21, and the fluorescence detection mechanism 43 has a scanning state for detecting the fluorescence signal of the nucleic acid in the microfluidic chip 21; and the control module 5 is electrically connected with the acquisition module 1, the chip module 2, the sample injection module 3 and the detection module 4, and the control module 5 can control the sample injection state, the driving state, the amplification state and the scanning state.
On the whole, when this automatic formula aerosol pathogen monitoring devices uses, set up collection module 1 at the target area, control aerosol collection device 11 through control module 5 and gather the aerosol sample in target area. The collected aerosol sample is delivered into the microfluidic chip 21 of the chip module 2 through the sample introduction module 3, the fluid in the microfluidic chip 21 can be driven to flow through the fluid driving mechanism 41 of the detection module 4, so that the operations of virus splitting, nucleic acid capturing, nucleic acid cleaning and the like can be performed on the aerosol sample by using the microfluidic chip 21, the nucleic acid amplification effect can also be realized by matching the nucleic acid amplification mechanism 42 of the detection module 4 with the microfluidic chip 21, and finally the fluorescence detection can be performed on the processed aerosol sample by using the fluorescence detection mechanism 43 of the detection module 4, so that the detection result of pathogens in the aerosol can be rapidly obtained.
Specifically, the designer can install the automatic aerosol pathogen monitoring device in a target area for use, such as an area with a large human flow rate, so as to monitor the aerosol pathogens in the target area. The control module 5 can control the acquisition module 1, the chip module 2, the sample injection module 3 and the detection module 4 to complete detection operation according to set time, for example, detection is performed every 4 hours.
In the embodiment of the present invention, as an example shown in fig. 2, the aerosol collecting device 11 includes a cyclone 111 and a sample container 112 disposed at a liquid phase outlet of the cyclone 111, and the sample container 112 is controllably communicated with the microfluidic chip 21 through the sample introduction pipe 31.
Specifically, the cyclone separator 111 includes a cyclone collection cylinder and a collection fan disposed on the cyclone collection cylinder. An aerosol sample of the target area can be efficiently collected by the cyclone 111 and discharged through the liquid phase outlet of the cyclone 111 into the sample container 112 for storage. The designer can adjust the sampling time of the cyclone 111 according to the sampling area to obtain the proper amount of aerosol samples.
In the embodiment of the present invention, as shown in fig. 4, the chip module 2 further includes a driving mechanism 22 and a chip tray 23 disposed on the driving mechanism 22, a plurality of microfluidic chips 21 are disposed, the microfluidic chips 21 are disposed on the chip tray 23 at intervals, and the driving mechanism 22 can drive the chip tray 23 to move, so as to drive at least one microfluidic chip 21 to enter the sample injection station.
Specifically, the driving mechanism 22 includes a tray slide rail 221 and a chip driving motor 222 disposed on the tray slide rail 221. The microfluidic chips 21 are arranged on the chip tray 23 at intervals to form a cartridge clip type structure, and the chip transmission motor 222 can drive the tray slide rail 221 to drive the chip tray 23 to move, so that the microfluidic chips 21 can be conveyed to a sample injection station one by the chip tray 23 for detection, the use convenience is improved, and a better use effect is achieved. The designer can determine the number of the microfluidic chips 21 according to the detection requirement, and is not limited to the specific example.
In the embodiment of the present invention, as shown in fig. 2, a sample feeding peristaltic pump 311 is disposed on the sample feeding pipeline 31, one end of the sample feeding pipeline 31 is communicated with the sample container 112, the other end of the sample feeding pipeline 31 is provided with a sample feeding hollow needle 315, and in a sample feeding state, the sample feeding hollow needle 315 is communicated with the microfluidic chip 21.
The sample feeding peristaltic pump 311 can be controlled by the control module 5, the aerosol sample in the sample container 112 can be rapidly injected into the microfluidic chip 21 through the sample feeding pipeline 31 by using the sample feeding peristaltic pump 311, and the aerosol sample is processed by using the microfluidic chip 21.
Specifically, the hollow sampling needle 315 may be combined with a pushing mechanism, the pushing mechanism is electrically connected to the control module 5, and the control module 5 controls the pushing mechanism to insert or withdraw the hollow sampling needle 315 into or out of the microfluidic chip 21.
In the embodiment of the present invention, along the direction from the sample container 112 to the sample hollow needle 315, the sample injection pipeline 31 is sequentially provided with a first one-way valve 312, a sample injection peristaltic pump 311, a bubble remover 313 and a flow meter 314.
Specifically, the first one-way valve 312 is a one-way electromagnetic pinch valve, and the opening and closing of the sample introduction pipeline 31 can be controlled by using the first one-way valve 312. The bubble remover 313 can remove bubbles in the aerosol sample, and avoid the bubbles from influencing the use of the microfluidic chip 21.
In the embodiment of the present invention, the sample injection module 3 further includes a collecting liquid pipeline 32, one end of the collecting liquid pipeline 32 is communicated with the sample container 112, the other end of the collecting liquid pipeline 32 is communicated with a collecting liquid container 321, and a second one-way valve 322 and a liquid inlet peristaltic pump 323 are sequentially arranged on the collecting liquid pipeline 32 along the direction from the sample container 112 to the collecting liquid container 321.
Specifically, the second one-way valve 322 is a one-way electromagnetic pinch valve, and the second one-way valve 322 can control the opening and closing of the collecting liquid pipeline 32. The collection liquid stored in collection liquid container 321 can be injected into sample container 112 by inlet peristaltic pump 323. The collection liquid contains pathogenic microorganism lysate, and can inactivate and lyse pathogenic microorganisms in the aerosol sample.
Further, still can set up liquid level sensor on collection liquid container 321, can acquire the liquid level of the collection liquid in collection liquid container 321 and upload to control module 5 through liquid level sensor to can control feed liquor peristaltic pump 323 through control module 5, be convenient for control the feed liquor volume of collection liquid.
In the embodiment of the present invention, the sample injection module 3 further includes a sample retention tube 33, one end of the sample retention tube 33 is provided with a sample retention container 331, the sample injection tube 31 located between the sample injection peristaltic pump 311 and the bubble remover 313 is provided with a first two-way valve 332, and the other end of the sample retention tube 33 is connected to the first two-way valve 332.
Specifically, the first two-way valve 332 is a two-way electromagnetic pinch valve. When the pathogen detection result is positive, the positive aerosol sample in the sample container 112 is pumped into the sample retention container 331 by the sample feeding peristaltic pump 311 to be retained by controlling the first two-way valve 332.
In the embodiment of the present invention, the sample injection module 3 further includes a waste liquid container 34, one end of the sample retention tube 33 is provided with a second two-way valve 341, and the waste liquid container 34 and the sample retention tube 331 are arranged at one end of the sample retention tube 33 in parallel through the second two-way valve 341.
Specifically, the second two-way valve 341 is a two-way electromagnetic pinch valve. When the pathogen detection result is negative, the remaining aerosol sample in the sample container 112 is pumped into the waste liquid container 34 by the sample feeding peristaltic pump 311 to be stored by controlling the second two-way valve 341.
In the embodiment of the present invention, the sample injection module 3 further includes a cleaning solution container 35, one end of the collecting liquid pipeline 32 is provided with a third two-way valve 351, and the cleaning solution container 35 and the collecting liquid container 321 are arranged in parallel at one end of the collecting liquid pipeline 32 through the third two-way valve 351.
Specifically, the third two-way valve 351 is a two-way electromagnetic pinch valve. The cleaning liquid container 35 stores cleaning liquid, and the cleaning liquid in the cleaning liquid container 35 can be pumped into the sample container 112 by the liquid inlet peristaltic pump 323 to clean the sample container 112. Then, the cleaning solution in the sample container 112 can be pumped into the waste liquid container 34 by the peristaltic pump 311.
In the embodiment of the present invention, as an example shown in fig. 5, the microfluidic chip 21 includes: a chip case 211; a sample tube unit 212, a wash tube unit 213, and a reagent tube unit 214 provided on the chip housing 211; a chip upper cover 215 and a chip lower 216 oppositely disposed at both ends of the chip housing 211, the chip lower 216 having a fluid pipeline communicating the sample tube unit 212, the wash tube unit 213 and the reagent tube unit 214; and a detection region 217 disposed on the chip lower 216, the detection region 217 being provided with a nucleic acid capturing filter paper.
The aerosol sample to be tested injected through the injection hollow needle 315 can be stored by the sample tube unit 212. The washing tube unit 213 can store the nucleic acid captured by the nucleic acid capturing filter paper and then perform impurity washing. Reagents used for detection, such as a Lamp reagent and a PCR reagent, can be stored in the reagent tube unit 214.
Specifically, in the embodiment shown in fig. 6, the sample tube unit 212 includes a sample tube 2121 disposed on the chip housing 211, and an inlet of the sample tube 2121 is provided with a rubber stopper 2122 and an annular packing 2123 covering the rubber stopper 2122. The middle part of the rubber plug 2122 can be arranged to be a concave structure, so that the hollow sampling needle 315 can pierce the rubber plug 2122 conveniently. The annular packing paper 2123 is hard packing paper, and the middle part of the annular packing paper 2123 can be penetrated by the sample injection hollow needle 315.
The wash pipe unit 213 includes wash pipes, and the reagent pipe unit 214 includes reagent pipes. The chip lower part 216 is provided with a thin hollow needle, and the chip lower part 216 can puncture the lower end rubber plugs 2122 of the sample tube, the cleaning tube and the reagent tube through the thin hollow needle, so that the fluid in the sample tube, the cleaning tube and the reagent tube can flow into the fluid pipeline for treatment. Specifically, 1ml of EPC water (DNase, RNase free) is placed in the washing tube (Cat: R0022, manufacturer: biyun Tian). The reagent tube is provided with PCR mix and primers and probe sets required by amplification of coronavirus, influenza A, influenza B and respiratory syncytial virus.
In an embodiment of the invention, the nucleic acid capture filter paper comprises a chitosan modified filter disposed at the detection zone 217 for nucleic acid capture. In the case of nucleic acid amplification detection, it is also necessary to heat and perform fluorescence detection on the detection region 217.
In another example, the designer can adjust the nucleic acid capture filter according to the nucleic acid capture needs, and is not particularly limited herein.
In the embodiment of the present invention, as shown in fig. 3, the fluid driving mechanism 41 includes a fluid driving jack 411 and a lifting driving motor 412 for driving the fluid driving jack 411 to lift, a sample injection station is disposed below the fluid driving jack 411, and in a driving state, the fluid driving jack 411 can be pressed on the sample tube unit 212 for fluid driving.
The annular packing paper 2123 can be pressed down by the fluid driving ejector 411, so as to drive the rubber plug 2122 to form a plunger type pressure driving mode in the sample tube, so as to perform fluid control on the microfluidic chip 21. The use mode of the cleaning tube and the reagent tube can refer to the sample tube, and is not described herein again.
In the embodiment of the present invention, the detection module 4 further includes a chamber selection motor 413, the chamber selection motor 413 is connected to the fluid driving mechanism 41 through a connecting member, and the chamber selection motor 413 can push the fluid driving mechanism 41 to move, so as to drive the fluid driving ram 411 to controllably press and connect with one of the sample tube unit 212, the wash tube unit 213, and the reagent tube unit 214.
The fluid driving mechanism 41 can be driven to move by using the chamber selection motor 413, so that the fluid driving ejector rod 411 is controlled to respectively press down the sample tube, the cleaning tube and the reagent tube on the microfluidic chip 21 to realize the processing process. The designer can determine the pressing sequence of the fluid-driven ejector pins 411 according to the fluid manipulation requirements of the microfluidic chip 21, and is not particularly limited herein.
In the using process, the microfluidic chip 21 is fixed on the chip tray 23, and the fluid in the microfluidic chip 21 is controlled by moving the fluid driving ejector rod 411, so that the mechanical error of the movement is concentrated on the fluid driving ejector rod 411. The micro-fluidic chip 21 is heated after the nucleic acid is extracted, and no mechanical displacement error is generated in the fluorescence detection process, so that the two problems that the nucleic acid amplification cannot be effectively carried out due to uneven temperature on the micro-fluidic chip 21 and the fluorescence signal detection is influenced due to inaccurate focal length in the fluorescence detection process when the micro-fluidic chip 21 is used for heating and amplifying the nucleic acid are solved.
In the embodiment of the present invention, the nucleic acid amplification mechanism 42 includes a heating structure 421 and a heat dissipation structure 422 disposed on the heating structure 421, and in the amplification state, the heating structure 421 can perform a temperature cycle process on the microfluidic chip 21 at the sample injection station.
Specifically, the heating structure 421 includes a heating block, and the heat dissipation structure 422 includes a water-cooling heat dissipation block disposed on the heating block. The outer side of the heating block is a copper block, and the inner part of the heating block is provided with a Peltier patch for generating temperature circulation required by nucleic acid amplification detection. The inside of water-cooling radiating block is equipped with miniature pump for dispel the heat to the heating block through the water-cooled, the required temperature when being convenient for adjust nucleic acid amplification. Through the heating block and the water-cooling radiating block, the required temperature for nucleic acid amplification can be better controlled, and the stability of nucleic acid amplification is improved.
In the embodiment of the present invention, the fluorescence detection mechanism 43 includes a four-color fluorescence detection unit 431 and a fluorescence scanning motor 432 for driving the four-color fluorescence detection unit 431, and in a scanning state, the four-color fluorescence detection unit 431 can perform fluorescence detection processing on the microfluidic chip 21 at the sample injection station.
The four-color fluorescence signals of FAM, VIC, ROX, CY5 can be read by the four-color fluorescence detection unit 431. The four-color fluorescence detection unit 431 can detect 4 indexes on the microfluidic chip 21. When the detection module 4 loads two microfluidic chips 21 at the same time, 8 indexes can be detected at the same time through the four-color fluorescence detection unit 431.
In the embodiment of the present invention, the control module 5 includes a main control unit (not shown), and a first sub-control unit 51 and a second sub-control unit 52 electrically connected to the main control unit, wherein the first sub-control unit 51 is electrically connected to the acquisition module 1, the chip module 2 and the sample injection module 3, and the second sub-control unit 52 is electrically connected to the detection module 4.
The detection process can be automatically controlled by matching the master control unit with the first sub-control unit 51 and the second sub-control unit 52, so that the detection operation of aerosol pathogens can be automatically completed according to a set program under the unattended condition, and the detection efficiency of the aerosol pathogens is greatly improved.
Second embodiment
The embodiment of the invention also provides an automatic aerosol pathogen monitoring system, which comprises at least one automatic aerosol pathogen monitoring device according to the first embodiment and a skynet module 6, wherein the detection module 4 is electrically connected with the skynet module 6, and the skynet module 6 has a monitoring state for acquiring a detection result of the detection module 4.
The specific structure, operation principle and beneficial effects of the automatic aerosol pathogen monitoring device are the same as those in the previous embodiment, and are not described herein again. This automatic formula aerosol pathogen monitoring system can accomplish the collection and the testing process of aerosol sample voluntarily through applying automatic formula aerosol pathogen monitoring devices, has obviously improved aerosol pathogen's detection efficiency.
Specifically, this automatic formula aerosol pathogen monitoring devices can set up a plurality ofly, can form aerosol pathogen detection net through setting up a plurality of automatic formula aerosol pathogen monitoring devices in different regions, has significantly improved automatic formula aerosol pathogen monitoring system to aerosol pathogen's monitoring capability to utilize day net module 6 can collect separately dynamic formula aerosol pathogen monitoring devices's testing result fast, the distribution that the person of being convenient for in time acquires the aerosol pathogen in each district. The designer can determine the number and the position of the automatic aerosol pathogen monitoring devices according to the monitoring needs of aerosol pathogens, and is not limited in particular.
Third embodiment
The embodiment of the invention provides a monitoring method using an automatic aerosol pathogen monitoring system, which comprises the following steps:
step 101: the collection module 1 is activated to collect an aerosol sample in air.
Step 102: the aerosol sample is injected into the microfluidic chip 21 of the chip module 2 by using the sample introduction line 31 of the sample introduction module 3.
Step 103: and carrying out temperature cycle processing and fluorescence detection processing on the microfluidic chip 21 through the detection module 4.
Step 104: and the detection result of the acquisition detection module 4 is uploaded to the skynet module 6.
Step 105: when the pathogen is detected, the detection result is positive, and the micro-fluidic chip 21 is replaced with a new micro-fluidic chip for rechecking.
Step 106: when the retest structure is negative, the skynet module 6 responds to the primary alarm.
Step 107: the skynet module 6 responds to a high-level alarm when the retest structure is positive.
In the embodiment of the present invention, before the collection module 1 is activated by the control module 5 to collect the aerosol sample in the air, the method further includes the following steps:
step 100: the control module 5 sends a reset instruction to reset the automatic aerosol pathogen monitoring device and the skynet module 6 to an initial state.
In the embodiment of the present invention, the acquiring, by the skynet module 6, the detection result of the detection module 4 further includes the following steps:
step 108: when the detection result is negative, the detection module 4 finishes the detection;
step 109: collecting waste liquid of the aerosol sample left in the acquisition module 1;
step 110: and cleaning the sample introduction pipeline 31 for the next detection.
Specifically, after the detection module 4 finishes detecting, the sample injection peristaltic pump 311 is started to pump the remaining aerosol sample into the waste liquid container 34, and then the liquid inlet peristaltic pump 323 is started to clean the sample injection pipeline 31 by using the cleaning liquid in the cleaning liquid container 35, so that the residue in the sample injection pipeline 31 is eliminated for the next detection.
All articles and references disclosed, including patent applications and publications, are hereby incorporated by reference for all purposes. The term "consisting essentially of 8230to describe a combination shall include the identified element, ingredient, component or step and other elements, ingredients, components or steps that do not materially affect the basic novel characteristics of the combination. The use of the terms "comprising" or "including" to describe combinations of elements, components, or steps herein also contemplates embodiments that consist essentially of such elements, components, or steps. By using the term "may" herein, it is intended to indicate that any of the described attributes that "may" include are optional. A plurality of elements, components, parts or steps can be provided by a single integrated element, component, part or step. Alternatively, a single integrated element, component, part or step may be divided into separate plural elements, components, parts or steps. The disclosure of "a" or "an" to describe an element, ingredient, component or step is not intended to foreclose other elements, ingredients, components or steps.
The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (19)
1. An automated aerosol pathogen monitoring device, comprising:
an acquisition module comprising an aerosol acquisition device;
a chip module comprising at least one microfluidic chip;
the sample injection module comprises a sample injection pipeline, and the sample injection pipeline has a sample injection state which is communicated with the aerosol acquisition device and the microfluidic chip;
the detection module comprises a fluid driving mechanism, a nucleic acid amplification mechanism and a fluorescence detection mechanism, wherein the fluid driving mechanism has a driving state for driving the fluid in the microfluidic chip to flow, the nucleic acid amplification mechanism has an amplification state for amplifying the nucleic acid in the microfluidic chip, and the fluorescence detection mechanism has a scanning state for detecting a fluorescence signal of the nucleic acid in the microfluidic chip; and
the control module is electrically connected with the acquisition module, the chip module, the sample injection module and the detection module, and can control the sample injection state, the driving state, the amplification state and the scanning state.
2. The automated aerosol pathogen monitoring device of claim 1, wherein the aerosol collection device comprises a cyclone separator and a sample container disposed at a liquid phase outlet of the cyclone separator, the sample container being controllably connected to the microfluidic chip via the sample introduction line.
3. The automatic aerosol pathogen monitoring device of claim 1 or 2, wherein the chip module further comprises a driving mechanism and a plurality of chip trays disposed on the driving mechanism, the plurality of microfluidic chips are disposed on the chip trays at intervals, and the driving mechanism can drive the chip trays to move to drive at least one of the microfluidic chips to enter the sample injection station.
4. The automatic aerosol pathogen monitoring device of claim 2, wherein a sample feeding peristaltic pump is disposed on the sample feeding pipeline, one end of the sample feeding pipeline is connected to the sample container, and the other end of the sample feeding pipeline is provided with a sample feeding hollow needle, and in the sample feeding state, the sample feeding hollow needle is connected to the microfluidic chip.
5. The automatic aerosol pathogen monitoring device of claim 4, wherein a first one-way valve, the sample peristaltic pump, a bubble trap and a flow meter are sequentially disposed on the sample inlet pipeline along the direction from the sample container to the sample inlet hollow needle.
6. The automatic aerosol pathogen monitoring device of claim 5, wherein the sample injection module further comprises a collection liquid pipeline, one end of the collection liquid pipeline is communicated with the sample container, the other end of the collection liquid pipeline is communicated with the collection liquid container, and a second one-way valve and a liquid inlet peristaltic pump are sequentially arranged on the collection liquid pipeline along the direction from the sample container to the collection liquid container.
7. The automated aerosol pathogen monitoring device of claim 6, wherein the sample introduction module further comprises a sample retention tube, wherein a sample retention container is disposed at one end of the sample retention tube, a first two-way valve is disposed on the sample introduction tube between the sample introduction peristaltic pump and the debubbler, and the other end of the sample retention tube is connected to the first two-way valve.
8. The automated aerosol pathogen monitoring device of claim 7, wherein the sample introduction module further comprises a waste liquid container and a cleaning solution container, wherein a second two-way valve is disposed at one end of the sample retention tube, and the waste liquid container and the sample retention container are disposed at one end of the sample retention tube in parallel through the second two-way valve; one end of the collection liquid pipeline is provided with a third two-way valve, and the cleaning liquid container and the collection liquid container are connected in parallel through the third two-way valve and are arranged at one end of the collection liquid pipeline.
9. The automated aerosol pathogen monitoring device of claim 1, wherein the microfluidic chip comprises:
a chip housing;
a sample tube unit, a washing tube unit and a reagent tube unit which are arranged on the chip shell;
the chip upper cover and the chip lower piece are oppositely arranged at two ends of the chip shell, the chip lower piece is provided with a fluid pipeline, and the fluid pipeline is communicated with the sample tube unit, the cleaning tube unit and the reagent tube unit; and
and the detection area is arranged on the lower piece of the chip and is provided with nucleic acid capture filter paper.
10. The automated aerosol pathogen monitoring device of claim 9, wherein the sample tube unit comprises a sample tube disposed on the chip housing, the sample tube inlet being provided with a plug and an annular packing paper covering the plug.
11. The automated aerosol pathogen monitoring device of claim 9 wherein the fluid drive mechanism comprises a fluid driven ram and a lift drive motor for driving the fluid driven ram to move up and down, the fluid driven ram being provided below the sample introduction station, the fluid driven ram being capable of being pressed against the sample tube unit for fluid driving in the driven state.
12. The automated aerosol pathogen monitoring device of claim 11 wherein the detection module further comprises a chamber selection motor, the chamber selection motor is connected to the fluid driving mechanism through a connector, the chamber selection motor can push the fluid driving mechanism to move, and the fluid driving ram is driven to controllably press against one of the sample tube unit, the wash tube unit and the reagent tube unit.
13. The automated aerosol pathogen monitoring device of claim 12, wherein the nucleic acid amplification mechanism comprises a heating structure and a heat dissipation structure disposed on the heating structure, wherein in the amplified state, the heating structure is capable of temperature cycling the microfluidic chip at the sample introduction station; the heating structure comprises a heating block, and the heat dissipation structure comprises a water-cooling heat dissipation block arranged on the heating block.
14. The automated aerosol pathogen monitoring device of claim 1 or 13, wherein the fluorescence detection mechanism comprises a four-color fluorescence detection unit and a fluorescence scanning motor for driving the four-color fluorescence detection unit, and in the scanning state, the four-color fluorescence detection unit can perform fluorescence detection processing on the microfluidic chip at the sample injection station.
15. The automated aerosol pathogen monitoring device of claim 1, wherein the control module comprises a master control unit, and a first sub-control unit and a second sub-control unit electrically connected to the master control unit, the first sub-control unit electrically connects the collection module, the chip module and the sample injection module, and the second sub-control unit electrically connects the detection module.
16. An automated aerosol pathogen monitoring system comprising at least one automated aerosol pathogen monitoring device of any of claims 1-15, and a skynet module, the detection module being electrically connected to the skynet module, the skynet module having a monitoring status that captures a detection result of the detection module.
17. A method of monitoring using an automated aerosol pathogen monitoring system, comprising the steps of:
starting an acquisition module to collect aerosol samples in the air;
injecting the aerosol sample into a microfluidic chip of a chip module by using a sample injection pipeline of a sample injection module;
carrying out temperature cycle processing and fluorescence detection processing on the microfluidic chip through a detection module;
acquiring a detection result of the detection module and uploading the detection result to an skynet module;
when a pathogen is detected, the detection result is positive, and the micro-fluidic chip is replaced with a new micro-fluidic chip for rechecking;
when the recheck structure is negative, the skynet module responds to a primary alarm;
the skynet module responds to a high-level alarm when the retest structure is positive.
18. The method of monitoring as claimed in claim 17, further comprising, before said activating, by the control module, the collection module to collect the aerosol sample in the air, the steps of:
and sending a reset instruction through the control module, and resetting the automatic aerosol pathogen monitoring device and the skynet module to an initial state.
19. The method of claim 18, wherein said obtaining the detection result of the detection module by the skynet module further comprises the steps of:
when the detection result is negative, the detection module finishes the detection;
collecting waste liquid of the aerosol sample left in the acquisition module;
and cleaning the sample injection pipeline for next detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211460504.2A CN115825026A (en) | 2022-11-17 | 2022-11-17 | Automatic aerosol pathogen monitoring device, system and monitoring method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211460504.2A CN115825026A (en) | 2022-11-17 | 2022-11-17 | Automatic aerosol pathogen monitoring device, system and monitoring method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115825026A true CN115825026A (en) | 2023-03-21 |
Family
ID=85529945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211460504.2A Pending CN115825026A (en) | 2022-11-17 | 2022-11-17 | Automatic aerosol pathogen monitoring device, system and monitoring method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115825026A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115725394A (en) * | 2022-11-17 | 2023-03-03 | 北京昌平实验室 | Aerosol sampling-loading device and method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527233A (en) * | 2015-12-10 | 2016-04-27 | 中国计量学院 | Determination device of hexavalent chromium in water sample based on microfluidic reaction system and determination method thereof |
| CN112285077A (en) * | 2020-10-12 | 2021-01-29 | 军事科学院系统工程研究院卫勤保障技术研究所 | Biological aerosol concentration monitoring device and monitoring method |
| US20210102265A1 (en) * | 2016-04-06 | 2021-04-08 | University Of Florida Research Foundation, Inc. | Bioaerosol detection systems and methods of use |
| US20210263056A1 (en) * | 2019-06-26 | 2021-08-26 | Beijing Boe Health Technology Co., Ltd. | Analysis device for detection chip, method for operating analysis device, and analysis system |
| CN113418757A (en) * | 2021-07-13 | 2021-09-21 | 北京鼎蓝科技有限公司 | Automatic detection device integrating bioaerosol sampling and nucleic acid detection |
| CN113611431A (en) * | 2021-08-02 | 2021-11-05 | 戚本昊 | Pre-inspection and sorting flow management system and method for new crown fever epidemic diseases |
| CN114181815A (en) * | 2021-12-06 | 2022-03-15 | 清华大学 | Large-flow ambient air bioaerosol sampling device and method |
| CN115322885A (en) * | 2022-08-16 | 2022-11-11 | 北京梓晶生物科技有限公司 | Integrated pathogen nucleic acid detection chip and nucleic acid detector with same |
-
2022
- 2022-11-17 CN CN202211460504.2A patent/CN115825026A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527233A (en) * | 2015-12-10 | 2016-04-27 | 中国计量学院 | Determination device of hexavalent chromium in water sample based on microfluidic reaction system and determination method thereof |
| US20210102265A1 (en) * | 2016-04-06 | 2021-04-08 | University Of Florida Research Foundation, Inc. | Bioaerosol detection systems and methods of use |
| US20210263056A1 (en) * | 2019-06-26 | 2021-08-26 | Beijing Boe Health Technology Co., Ltd. | Analysis device for detection chip, method for operating analysis device, and analysis system |
| CN112285077A (en) * | 2020-10-12 | 2021-01-29 | 军事科学院系统工程研究院卫勤保障技术研究所 | Biological aerosol concentration monitoring device and monitoring method |
| CN113418757A (en) * | 2021-07-13 | 2021-09-21 | 北京鼎蓝科技有限公司 | Automatic detection device integrating bioaerosol sampling and nucleic acid detection |
| CN113611431A (en) * | 2021-08-02 | 2021-11-05 | 戚本昊 | Pre-inspection and sorting flow management system and method for new crown fever epidemic diseases |
| CN114181815A (en) * | 2021-12-06 | 2022-03-15 | 清华大学 | Large-flow ambient air bioaerosol sampling device and method |
| CN115322885A (en) * | 2022-08-16 | 2022-11-11 | 北京梓晶生物科技有限公司 | Integrated pathogen nucleic acid detection chip and nucleic acid detector with same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115725394A (en) * | 2022-11-17 | 2023-03-03 | 北京昌平实验室 | Aerosol sampling-loading device and method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208362349U (en) | molecular diagnostic device | |
| CN101688169B (en) | Device for lysing microorganisms present in an environmental or clinical sample and extracting nucleic acids from the microorganisms for analysis | |
| CN115825026A (en) | Automatic aerosol pathogen monitoring device, system and monitoring method | |
| JP2008005840A (en) | Method and unit for preparing liquid sample for analyzing microorganism | |
| JP2013509173A (en) | Devices and equipment | |
| CN113477282A (en) | Single cell separation system and method based on droplet microfluidics | |
| JP6207815B2 (en) | Filtering member and filtering method | |
| CN115093961A (en) | Multi-volume liquid drop digital LAMP nucleic acid absolute quantitative detection device and method and application | |
| US20220056390A1 (en) | Bacteria separation system and methods | |
| JP2018061451A (en) | Nucleic acid extraction device and nucleic acid extraction method | |
| WO2017203744A1 (en) | Nucleic acid examination device | |
| JP6771161B2 (en) | Nucleic acid extractor | |
| KR101684689B1 (en) | Blood collection device | |
| CN103602583A (en) | Integrated multifunctional microfluidic chip | |
| KR20100009052A (en) | Method and apparatus for automatically analyzing dan of microbe in food | |
| CN216688084U (en) | Nucleic acid extraction detection device | |
| CN215288792U (en) | Nucleic acid diagnosis micro-fluidic chip | |
| CN213416841U (en) | Circulating tumor cell sorter | |
| CN115612593A (en) | Full-integrated nucleic acid detection micro-fluidic chip and nucleic acid detector with same | |
| KR101908836B1 (en) | Apparatus for capturing bacteria | |
| CN214694209U (en) | Full-automatic fluorescence quantitative PCR instrument | |
| WO2000077253A1 (en) | Apparatus and method for gene examination | |
| CN112609021B (en) | Aspergillus flavus RPA primer, kit, and aspergillus flavus detection method and device | |
| CN115261440B (en) | Functional liquid, system and method for detecting total number of culturable bacteria in prawn body | |
| US20220056397A1 (en) | Cell analysis systems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |