CN115818621A - Biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristic and preparation method and application thereof - Google Patents
Biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristic and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a biomass-derived carbon nano particle with near-infrared two-region light emission characteristics and a preparation method and application thereof, belonging to the technical field of carbon nano materials, wherein a nitrogen source, a boron source and a biomass carbon source are subjected to dehydration carbonization reaction in a polar solution to prepare boron/nitrogen co-doped carbon nano particles; and then, coating the carbon nano particles by using a film coating material to enhance the water solubility of the carbon nano particles. The prepared carbon nano particles can be excited under laser with the wavelength of 808nm, show obvious NIR-II emission from 900 to 1200nm, and have efficient photo-thermal conversion characteristics; the carbon nano-particles have good biocompatibility and can be applied to the technical fields of near-infrared two-region fluorescence biological imaging, cancer photo-thermal treatment and the like; meanwhile, the preparation method is simple, has lower process requirements and can realize quantitative production.
Description
Technical Field
The invention belongs to the technical field of carbon nano materials, and particularly relates to a biomass-derived carbon nano particle with near-infrared two-region light emission characteristics, and a preparation method and application thereof.
Background
Fluorescence imaging is a non-invasive in vivo imaging technique that provides rapid feedback in real time, high sensitivity and harmless radiation, and thus has great advantages in visualizing the anatomy and function of organs. In recent years, the fluorescence imaging technology of the near infrared two-region (NIR-II, 1000-1700 nm) becomes a popular imaging technology for basic research and clinical application, and has strong application potential in the biomedical field. Compared to visible/near-infrared-first (NIR-I) based bioimaging, NIR-II fluorescence imaging provides high signal-to-noise ratio and high spatial and temporal resolution for deep tissue imaging due to its moderate tissue absorption and relatively low tissue scattering, minimized autofluorescence, and negligible phototoxicity to living cells, and enables direct visualization and real-time monitoring of deep biological structures and processes with clarity.
In recent years, fluorescent carbon nanomaterials emitting in the near infrared region have become promising candidates for biomedical assays and therapies due to minimal damage to biological matrices, large tissue penetration depths and minimal autofluorescence background of biological samples. However, most of the recently reported fluorescent Carbon Nanoparticles (CNPs) triggered by Ultraviolet (UV)/blue light exhibit NIR-I emission shorter than 820nm, seriously hindering their application in vivo. There is still a lack of efficient methods for preparing CNPs emitting NIR-II. Furthermore, in the only reports on CNPs emitting NIR-I, the preparation methods of these CNPs are complicated and the biocompatibility is not ideal. The selection of carbon sources is very important in the preparation of CNPs, and biomass carbon sources from nature have the advantages of low price, no toxicity, wide application and the like. Therefore, it is very important to develop NIR-triggered CNPs based on biomass carbon sources, having a simple preparation method, low process requirements, quantitative production, good biocompatibility, and NIR-II emission.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide biomass-derived carbon nanoparticles with near-infrared two-region light emission characteristics and a preparation method and application thereof.
In order to achieve the purpose of the invention, the invention adopts the technical scheme that:
a biomass-derived carbon nanoparticle having near-infrared two-region light emission characteristics, wherein: the biomass-derived carbon nanoparticles are composed of boron/nitrogen co-doped carbon nanoparticles and a coating material wrapped on the surface, the coating material is one or more of hyaluronic acid, bovine serum albumin, polyvinyl alcohol or polyvinylpyrrolidone, the crystal diameter of the biomass-derived carbon nanoparticles is 5-200nm, the biomass-derived carbon nanoparticles are excited under laser with the wavelength of 808nm, the biomass-derived carbon nanoparticles are emitted in an obvious near-infrared two-region with the wavelength of 900nm-1200 nm, and the peak center of a wave peak is 928nm.
Further, the nitrogen source is one or more of boron nitride, borazene, 3-aminobenzeneboronic acid, urea, melamine or ammonium citrate; the boron source is one or more of boron nitride, borazene, 3-aminophenylboronic acid, granular borax or anhydrous sodium tetraborate; the biomass carbon source is one or more of grapes, pears, watermelons, kiwi fruits, apples or strawberries.
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, squeezing a biomass carbon source to obtain juice, performing centrifugal separation on the obtained solution at the rotating speed of 3000 to 12000r/min, taking supernate, and discarding precipitates;
s2, dissolving a nitrogen source and a boron source into absolute ethyl alcohol together, wherein the mass volume ratio of the nitrogen source to the boron source to the absolute ethyl alcohol is (100 mg-1000 mg): (100 mg-1000 mg): 1mL, uniformly dispersing by using ultrasonic, wherein the frequency of ultrasonic dispersion is 50KHz, and the time of ultrasonic dispersion is 30-60min, then adding the obtained product into the supernatant obtained in the step S1, and stirring at the temperature of 28 ℃ for 5-30min to obtain a mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a polytetrafluoroethylene high-pressure reaction kettle, reacting for 3 to 10 hours at the temperature of 100 to 200 ℃, and then centrifugally separating and freeze-drying the obtained solution at the rotating speed of 5000 to 12000r/min to prepare boron/nitrogen co-doped carbon nano particle powder;
s4, adding the film coating material into deionized water, wherein the mass-volume ratio of the film coating material to the deionized water is (20 mg-100 mg): 1mL, stirring at high speed after ultrasonic dispersion until the solution is in a transparent state, wherein the ultrasonic dispersion frequency is 50KHz, the ultrasonic dispersion time is 5min, the stirring temperature is 28 ℃, and the stirring time is 5min, so as to prepare a film-coating material solution;
s5, preparing the boron/nitrogen co-doped carbon nano particle powder prepared in the step S3 into a uniformly dispersed boron/nitrogen co-doped carbon nano particle aqueous solution; then slowly dripping the boron/nitrogen co-doped carbon nanoparticle aqueous solution into the coating material solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 5 to 15h; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 5000 to 12000r/min, and thus the biomass-derived carbon nanoparticles with the near-infrared two-region light emission characteristic are prepared.
A biomass-derived carbon nanoparticle with near-infrared two-region light emission characteristics is applied to fluorescence imaging, photo-thermal therapy, infrared anti-counterfeiting or drug carriers.
Compared with the prior art, the invention has the beneficial effects that:
1. the biomass-derived carbon nano particles with the near-infrared two-region light emission characteristic are formed by dehydration carbonization reaction of a nitrogen source, a boron source and a biomass carbon source in a polar solution; the prepared biomass-derived carbon nanoparticles can be excited under laser with the wavelength of 808nm, and exhibit obvious NIR-II emission from 900 to 1200nm, and have efficient photo-thermal conversion characteristics;
2. the biomass-derived carbon nanoparticles have low toxicity and good biocompatibility;
3. the invention has low manufacturing cost and simple preparation method, and can be used for preparing biomass-derived carbon nanoparticles in large scale.
Drawings
FIG. 1 is a transmission electron micrograph (TME) of the CNPs synthesized in example 1;
FIG. 2 is a hydrated particle size histogram of the CNPs synthesized in example 1;
FIG. 3 is a Fourier Infrared (FTIR) absorption spectrum of the CNPs synthesized in example 1;
FIG. 4 is a NIR-II fluorescence spectrum and a fluorescence spectrum thereof of CNPs synthesized in example 1;
FIG. 5 is a visible-near infrared two-domain absorption spectrum of the CNPs synthesized in example 1;
FIG. 6 is a chart of Balb/c mouse NIR-II fluorescence in vivo imaging of CNPs synthesized in example 1;
fig. 7 is a graph showing photothermal properties of the CNPs synthesized in example 1.
Detailed Description
The invention is described in further detail below with reference to the figures and examples.
Example 1
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of watermelon juice into a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and removing sediment;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring the mixture at the stirring temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared, and can be applied to fluorescence imaging, photothermal therapy, infrared anti-counterfeiting or drug carriers.
In this example 1, the prepared bovine serum albumin-coated carbon nanoparticles were dissolved in deionized water, and administered by rat tail intravenous injection to detect the imaging performance of the carbon nanoparticles.
Fig. 1 is a Transmission Electron Microscope (TEM) image of the CNPs synthesized in example 1. As can be seen, the synthesized CNPs have regular morphology, uniform particle size distribution and a diameter of about 38nm.
Fig. 2 is a hydrated particle size histogram of the CNPs synthesized in example 1. As can be seen from the graph, the synthesized CNPs have uniform particle size, and about 94.7% of the CNPs have a hydrated particle size of 190nm.
Fig. 3 is a fourier infrared (FTIR) absorption spectrum of the CNPs synthesized in example 1. As can be seen, 3218cm -1 、2912cm -1 、1498cm -1 、1074cm -1 And 751cm -1 Absorption peaks at the position of the compound are respectively belonging to N-H, C-H, B-O, C-O and B-O-B telescopic vibration, which proves that the B/N-CNPs are successfully synthesized, and the surface of the compound is rich in functional groups containing oxygen, nitrogen and boron.
FIG. 4 is a NIR-II fluorescence spectrum and a fluorescence spectrum thereof of CNPs synthesized in example 1. As can be seen, the synthesized CNPs exhibited significant NIR-II emission at wavelengths from 900 to 1200nm with a peak centered at 928nm.
Fig. 5 is a visible-near infrared two-domain absorption spectrum of the CNPs synthesized in example 1. As can be seen, two distinct absorption peaks are shown at about 230nm and 285nm in the UV region, and one at about 1153nm in the NIR-II region.
FIG. 6 is a chart of Balb/c mouse NIR-II fluorescence in vivo imaging of CNPs synthesized in example 1. As can be seen from the figure, after Balb/c mice are administrated in the tail vein, the fluorescence intensity of the lung and the liver of the mice is still kept at a higher level for 6h, and particularly, the lung targeting imaging capability is better.
Fig. 7 is a graph showing photothermal properties of the CNPs synthesized in example 1. As can be seen from the graph, solutions of CNPs of different concentrations all exhibited rapid temperature rise under irradiation of laser light having a wavelength of 1064nm, whereas the temperature of pure water remained almost unchanged.
Example 2
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of kiwi fruit juice in a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and removing sediment;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring the mixture at the stirring temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolving and dispersing, wherein the frequency is 50KHz, the dispersing time is 5min, and stirring the mixture for 5min at the temperature of 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying at the rotating speed of 6000r/min to prepare the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic.
Example 3
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of grape juice in a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and discarding precipitates;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring at the temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then carrying out centrifugal separation and freeze drying on the obtained solution, wherein the centrifugal separation rotating speed is 6000r/min, so as to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared.
Example 4
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of strawberry juice into a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and discarding precipitates;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring the mixture at the stirring temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared.
Example 5
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 40mL of watermelon juice into a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 5000r/min, taking supernate, and discarding precipitates;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring at the temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 200 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared.
Example 6
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of watermelon juice into a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and removing precipitates;
s2, adding 1800mg of boron nitride into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring the mixture at the stirring temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of bovine serum albumin into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of the B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the bovine serum albumin coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared.
Example 7
A preparation method of biomass-derived carbon nanoparticles with near-infrared two-zone light emission characteristics comprises the following steps:
s1, placing 35mL of watermelon juice into a centrifugal tube for centrifugal separation, wherein the centrifugal separation rotating speed is 4000r/min, taking supernate, and removing sediment;
s2, adding 1500mg of 3-aminophenylboronic acid into 5mL of absolute ethyl alcohol, placing the mixture into an ultrasonic dispersion instrument for ultrasonic dispersion uniformly, wherein the ultrasonic dispersion frequency is 50KHz, and the ultrasonic dispersion time is 50min, then adding the mixture into the supernatant prepared in the step S1, and stirring the mixture at the stirring temperature of 28 ℃ for 5min to prepare a milky mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a 100mL polytetrafluoroethylene high-pressure reaction kettle, reacting for 8 hours at the temperature of 180 ℃, and then performing centrifugal separation and freeze drying on the obtained solution at the centrifugal separation rotating speed of 6000r/min to prepare boron/nitrogen co-doped carbon nano particle powder (B/N-CNPs);
s4, adding 1200mg of hyaluronic acid into 200mL of deionized water, placing the mixture into an ultrasonic dispersion instrument for dissolution and dispersion, wherein the frequency is 50KHz, the dispersion time is 5min, and stirring the mixture for 5min at 28 ℃ until the solution is in a transparent state to prepare a bovine serum albumin aqueous solution;
s5, adding 400mg of B/N-CNPs powder prepared in the step S3 into 40mL of deionized water to prepare a B/N-CNPs aqueous solution which is uniformly dispersed and has the mass concentration of 10 mg/mL; then slowly dripping the B/N-CNPs aqueous solution into the bovine serum albumin aqueous solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 10 hours; and finally, washing, centrifugally separating, freezing and drying, wherein the rotational speed of centrifugal separation is 6000r/min, and the hyaluronic acid coated carbon nano-particles with the near-infrared two-region light emission characteristic are prepared.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (4)
1. A biomass-derived carbon nanoparticle having near-infrared two-region light emission characteristics, comprising: the biomass-derived carbon nanoparticles are composed of boron/nitrogen co-doped carbon nanoparticles and a coating material wrapped on the surface, the coating material is one or more of hyaluronic acid, bovine serum albumin, polyvinyl alcohol or polyvinylpyrrolidone, the crystal diameter of the biomass-derived carbon nanoparticles is 5-200nm, the biomass-derived carbon nanoparticles are excited under laser with the wavelength of 808nm, the biomass-derived carbon nanoparticles are emitted in an obvious near infrared two-region with the wavelength of 900nm-1200 nm, and the peak central wavelength of a peak is 928nm.
2. The biomass-derived carbon nanoparticle with near-infrared two-region light emission characteristics according to claim 1, wherein: the nitrogen source is one or more of boron nitride, borazene, 3-aminobenzeneboronic acid, urea, melamine or ammonium citrate; the boron source is one or more of boron nitride, borazene, 3-aminophenylboronic acid, granular borax or anhydrous sodium tetraborate; the biomass carbon source is one or more of grapes, pears, watermelons, kiwi fruits, apples or strawberries.
3. A method for preparing biomass-derived carbon nanoparticles having near-infrared two-region light emission characteristics according to claim 1 or 2, comprising the steps of:
s1, squeezing a biomass carbon source to obtain juice, performing centrifugal separation on the obtained solution at the rotating speed of 3000 to 12000r/min, taking supernate, and discarding precipitates;
s2, dissolving the nitrogen source and the boron source into absolute ethyl alcohol together, wherein the mass-volume ratio of the nitrogen source to the boron source to the absolute ethyl alcohol is (100 mg-1000 mg): (100 mg-1000 mg): 1mL, uniformly dispersing by using ultrasonic, wherein the frequency of ultrasonic dispersion is 50KHz, and the time of ultrasonic dispersion is 30-60min, then adding the obtained product into the supernatant obtained in the step S1, and stirring at the temperature of 28 ℃ for 5-30min to obtain a mixed solution;
s3, transferring the mixed solution prepared in the step S2 into a polytetrafluoroethylene high-pressure reaction kettle, reacting for 3 to 10 hours at the temperature of 100 to 200 ℃, and then centrifugally separating and freeze-drying the obtained solution at the rotating speed of 5000 to 12000r/min to prepare boron/nitrogen co-doped carbon nano particle powder;
s4, adding the film coating material into deionized water, wherein the mass-volume ratio of the film coating material to the deionized water is (20 mg-100 mg): 1mL, stirring at high speed after ultrasonic dispersion until the solution is in a transparent state, wherein the ultrasonic dispersion frequency is 50KHz, the ultrasonic dispersion time is 5min, the stirring temperature is 28 ℃, and the stirring time is 5min, so as to prepare a film-coating material solution;
s5, preparing the boron/nitrogen co-doped carbon nano particle powder prepared in the step S3 into a uniformly dispersed boron/nitrogen co-doped carbon nano particle aqueous solution; then slowly dripping the boron/nitrogen co-doped carbon nanoparticle aqueous solution into the coating material solution prepared in the step S4, and fully stirring at the stirring temperature of 28 ℃ for 5 to 15h; and finally, washing, centrifugally separating, and freeze-drying at the rotation speed of 5000 to 12000r/min to prepare the biomass-derived carbon nanoparticles with the near-infrared two-region light emission characteristic.
4. Use of biomass-derived carbon nanoparticles having near-infrared two-region light emission properties according to claim 1 or 2, wherein: the biomass-derived carbon nanoparticles are applied to fluorescence imaging, photothermal therapy, infrared anti-counterfeiting or drug carriers.
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