CN115791253A - Sample slice auxiliary device for electron microscope - Google Patents
Sample slice auxiliary device for electron microscope Download PDFInfo
- Publication number
- CN115791253A CN115791253A CN202211307385.7A CN202211307385A CN115791253A CN 115791253 A CN115791253 A CN 115791253A CN 202211307385 A CN202211307385 A CN 202211307385A CN 115791253 A CN115791253 A CN 115791253A
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- China
- Prior art keywords
- hair roller
- sliding rail
- extending end
- electron microscope
- extending
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 210000004209 hair Anatomy 0.000 claims abstract description 51
- 239000002184 metal Substances 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 21
- 239000000463 material Substances 0.000 description 10
- 238000003825 pressing Methods 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 3
- 238000002788 crimping Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sample slicing auxiliary device for an electron microscope, which comprises a base, a bracket, a hair roller and a pressure lever, wherein the hair roller is arranged on the base; the right side of the base is provided with an upper sliding rail and a lower sliding rail, and the left side of the bracket is arranged in the upper sliding rail and the lower sliding rail in a sliding manner; the front end and the rear end of the right side of the bracket are respectively provided with a transverse extending end, and the two ends of the hair roller are arranged on the inner side of the extending end in a sliding manner through strip-shaped sliding blocks; a reset torsion spring is connected between the tail end of the hair roller and the strip-shaped sliding block; a second extending end is arranged at the right end of each extending end, and a second spring is arranged between the pressure rod and the second extending end; a wiring hole for connecting the left end of the hair roller slide rail and the top end of the pressure rod slide rail is arranged in each extending end and the second extending end, a pull wire is arranged in the wiring hole, one end of the pull wire is fixed and wound at one end of the hair roller, and the other end of the pull wire is fixedly connected with the pressure rod; the base is provided with a fixing mechanism. The invention realizes automatic anti-rolling, and saves time and labor.
Description
Technical Field
The invention relates to the technical field of biological specimen frozen sections of an electron microscope, in particular to a sample section auxiliary device for the electron microscope.
Background
The problem of slice winding can occur during slicing because a freezing microtome is required when biological samples of an electron microscope are frozen and sliced. To this problem, the section personnel adopt the supplementary section of writing brush to replace to prevent rolling up the board more, and is specific, uses the nib of writing brush to sweep lightly and press sliced turn-up when the section, makes the turn-up unfold sweep flat, can effectual reduction turn-up degree. However, the operation assisted by the writing brush is also insufficient, and firstly, the manipulation of an operator is examined, which wastes time and labor. Secondly, only one side of the slice can be lightly swept by using a writing brush, the other side of the slice cannot be touched, when the curled edge on the other side is unfolded, the curled edge on one side can be pressed by using the writing brush, and then the curled edge on the other side is quickly pressed, so that the operation is troublesome, and the slice is easily damaged.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the sample slice auxiliary device special for the anti-rolling of the ultrathin slice for the electron microscope, which realizes the automatic anti-rolling and saves time and labor.
The technical scheme adopted by the invention for solving the technical problems is as follows: a sample slice auxiliary device for an electron microscope comprises a base, a bracket, a hair roller and a pressure lever; an upper sliding rail and a lower sliding rail are arranged on the right side of the base, the left side of the support is slidably arranged in the upper sliding rail and the lower sliding rail, and a locking mechanism is arranged between the upper sliding rail and the support and between the upper sliding rail and the lower sliding rail and the support; the front end and the rear end of the right side of the bracket are respectively provided with a transverse extending end, the two ends of the hair roller are arranged on the inner side of the extending end in a sliding manner through strip-shaped sliding blocks, and a spring is arranged between the extending end and the strip-shaped sliding blocks in a sliding manner; a reset torsion spring is connected between the tail end of the hair roller and the strip-shaped sliding block; a second extending end which inclines to the lower left is arranged at the right end of each extending end, the front end and the rear end of the pressure lever can be respectively arranged at the inner side of the corresponding second extending end in a vertically sliding manner, and a second spring is arranged between the pressure lever and the second extending end; a wiring hole for connecting the left end of the hair roller slide rail and the top end of the pressure rod slide rail is arranged in each of the extension end and the second extension end, a pull wire is arranged in the wiring hole, one end of the pull wire is fixed and wound at one end of the hair roller, and the other end of the pull wire is fixedly connected with the pressure rod; the base is provided with a fixing mechanism.
In order to realize the synchronous sweeping of the slicing and the hair roller, an adjusting bolt is further rotatably arranged on the right side of the pressure lever.
For convenience of operation, the fixing mechanism is preferably a manual suction cup.
Furthermore, a plurality of pulleys matched with the pull wire are arranged in the wire feeding hole.
Furthermore, locking mechanism includes magnet I and the magnet II of I complex of magnet on the support of upper and lower slide rail top.
The invention has the beneficial effects that: compared with manual operation in the prior art, the anti-rolling device does not need manual operation, does not depend on the proficiency of an operator, and can ensure that the anti-rolling effect of each slice does not have errors or errors. It replaces artifical handheld writing brush operation through the brush roller that can rotate and gliding, and the brush roller can contact with whole sliced crimping, has solved manual operation and can only sweep the problem of flat section one side crimping.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is an elevational, cross-sectional view of the present invention;
FIG. 4 is an enlarged view of detail A of FIG. 3;
FIG. 5 is a front view of a hair roller;
FIG. 6 is a schematic top view of a wool roller and a strip slip of the present invention;
FIG. 7 is a schematic view of a hair roller sweeping sample slice of the present invention;
in the figure: the device comprises a base 1, a fixing mechanism 11, a sliding chute 12, an upper sliding rail 13, a lower sliding rail 13, a transverse sliding rail 14, a support 2, a support sliding block 21, an extending end 22, a hair roller sliding rail 23, a strip sliding block 24, a sliding block wire feeding hole 241, a spring 25, a second extending end 26, a pressure lever sliding rail 27, a second spring 28, a wire feeding hole 29, a pull wire 291, a pulley 292, a hair roller 3, bristles 31, a roller body 32, a reset torsion spring 33, a bearing 34, a pressure lever 4, a material receiving platform 5, a sample chuck 6, a sample 61, an adjusting bolt 7 and a sample slice 8.
Detailed Description
For a better understanding of the present invention, embodiments of the present invention are explained in detail below with reference to fig. 1 to 7.
It should be noted that the directions "front (front), back (back), left, right, up, down" described herein are based on the directions "front, back, left, right, up, down" in fig. 1.
The invention relates to a sample slice auxiliary device for an electron microscope, which comprises a base 1, a bracket 2, a hair roller 3 and a pressure lever 4. The left side of base 1 sets up fixed establishment 11, fixed establishment 11 adopts manual sucking disc or manual magnet switch, fixes base 1 on connecing material platform 5 through suction. The right side of the base 1 is provided with a sliding groove 12 matched with the support 2, the right side and the top of the sliding groove 12 are open, the front side and the back side of the sliding groove 12 are oppositely provided with an upper sliding rail 13 and a lower sliding rail 13, the front side and the back side of the support 2 are respectively provided with a support sliding block 21, the support 2 slides in the upper sliding rail 13 and the lower sliding rail 13 through the support sliding blocks 21 on the two sides, a locking mechanism is arranged between the support and the upper sliding rail 13, the locking mechanism can adopt a transverse sliding rail 14, the top end of the upper sliding rail 13 and the top end of the lower sliding rail 13 are provided with a transverse sliding rail 14, furthermore, the tail end of the transverse sliding rail 14 can be provided with a groove so as to be capable of hanging the support sliding block 21, of course, the locking mechanism of the invention can be provided with no transverse sliding rail 14, and the support sliding block 21 is provided with a hook or other connecting pieces, after the support sliding block 21 is lifted to the top ends of the upper sliding rail 13 and the lower sliding rail 13, the support sliding block 21 can be hung on the top ends of the upper sliding rail 13 by means of magnets. The second extending end 26 can be close to the material receiving platform 5 by adopting the magnet for adsorption fixation, and a space for moving leftwards does not need to be reserved.
It should be noted that, if the second protruding end 26 is close to the receiving platform 5, since the second protruding end 26 is affected by the receiving platform 5 when moving upwards, the upper and lower sliding rails 13 need to be designed as inclined rails parallel to the second protruding end 26 as shown in fig. 2 so as to be able to be lifted up smoothly. And for sufficient space between the second protruding end 26 and the receiving platform 5, the upper and lower slide rails 13 can be designed in the vertical state of fig. 1 and 3.
The front side and the rear side of the right side of the bracket 2 are respectively fixedly connected with a transverse extending end 22, and the inner side of each extending end 22 is provided with a hair roller slide rail 23.
As shown in fig. 6, the two ends of the hair roller 3 are provided with shaft ends, the tail ends of the shaft ends are connected with a reset torsion spring 33 (a clockwork spring), and the shaft ends are rotatably arranged on the strip-shaped sliding blocks 24 through bearings 34. One end of the return torsion spring 33 is fixed on the strip-shaped sliding block 24, and the other end is fixed at the shaft end. The force of the reset torsion spring 33 is smaller than the jacking force of the spring 25, so that when the stay 291 is pulled, the hair roller 3 overcomes the force of the reset torsion spring 33 to rotate the hair roller 3, and after the stay 291 is pulled to the bottom, the hair roller 3 is pulled to move leftwards by overcoming the force of the spring 25. Such as the hair roller 3 shown in fig. 5. The hair roller 3 is arranged with layer-by-layer brush hair 31 in a rotating array by an axial lead, the brush hair 31 is obliquely arranged in a clockwise direction, the brush hair 31 can be rabbit hair, and the brush hair is gradually thinned from the root part of the inner side to the outside. The hair roller 3 is preferably made of a metal block, the hair roller 3 made of metal can be kept in an ice-cold state due to low temperature in a slicer operating room, and the bristles 31 are firstly fixed on a layer of cloth, and then the cloth is fixed on the hair roller. A spring 25 is arranged in each hair roller slide rail 23, and the spring 25 is positioned between the hair roller slide rail 23 and the strip-shaped slide block 24. A second extending end 26 which is inclined towards the lower left is fixedly connected to the right end of each extending end 22, and as shown in fig. 2, the extending ends 22 and the second extending ends 26 are attached to two surfaces of the material receiving platform 5. The inner side of the second extending end 26 is provided with a pressing rod slide rail 27, the front end and the rear end of the pressing rod 4 are respectively slidably mounted in the corresponding pressing rod slide rail 27, and the right end of the pressing rod 4 is provided with an extending end for pressing down the sample chuck 6.
Because the size of the sample 61 to be sliced is different, in order to adjust the triggering time of the pressure lever 4, an adjusting bolt 7 is arranged on the right side of the pressure lever 4 in a threaded connection mode, and the adjusting bolt 7 is rotated to be lifted on the pressure lever 4, so that the distance between the top of the adjusting bolt 7 and the sample chuck 6 is adjusted. For example, when the sample 61 is small, the sample chuck 6 moves downwards to touch the adjusting bolt 7, so that the hair roller 3 moves leftwards after the stay wire at the shaft end of the hair roller 3 is straightened, but at the moment, the blade does not start to cut the sample, so that the hair roller 3 cannot function as a flat-cutting blade, and in this case, the sample chuck 6 touches the adjusting bolt 7 when the sample 61 just contacts the blade by moving the adjusting bolt 7 downwards.
A second spring 28 is placed in the pressure rod slide rail 27, the top of the second spring 28 supports the bottom of the pressure rod 4, a wire running hole 29 which is connected with the left end of the hair roller slide rail 23 and the top end of the pressure rod slide rail 27 is arranged in each extending end 22 and each second extending end 26, a pull wire 291 is arranged in each wire running hole 29, one end of the pull wire 291 penetrates through the bottom of the hair roller 3 rightwards and is fixedly connected with the shaft end after being wound around the shaft end (only 1-2 circles) of the hair roller 3 anticlockwise, the strip-shaped slide block 24 is provided with a slide block wire running hole 241, and the other end of the pull wire 291 penetrates through the center of the slide block wire running hole 241 and the spring 25 and is fixedly connected to the pressure rod 4 after passing through the pulley 292. As shown in fig. 3 and 4, 2 pulleys 292 matched with the pulling wire 291 are arranged in the wire running hole 29, and the pulleys 292 are arranged at the turning of the wire running hole 29 to avoid the direct contact between the pulling wire 291 and the wire running hole 29, thereby saving labor and increasing the service life of the pulling wire 291.
When the material receiving platform is used, the base 1 is fixedly adsorbed on the material receiving platform 5 through the manual sucker, the extending end 22 of the support 2 is parallel to the top surface of the material receiving platform 5, and the second extending end 26 is parallel to the right side surface of the material receiving platform 5, so that the installation is completed. In the next slicing process, when the sample chuck 6 moves downwards, the adjusting bolt 7 is gradually touched, so that the press rod 4 synchronously slides downwards along with the sample chuck 6 in the press rod slide rail 27, the press rod 4 pulls the hair roller 3 to rotate firstly through the pull wire 291, the cut sample slice 8 is swept straight, after the pull wire wound at the shaft end of the hair roller 3 is straightened, the hair roller 3 slides leftwards in the hair roller slide rail 23, and meanwhile, the sample slice 8 also slides leftwards.
After the material cutting action is finished, the bracket 2 is lifted by hands and moved to the left, so that the bracket slide block 21 of the bracket 2 slides into the transverse slide rail 14 from the upper slide rail 13 and the lower slide rail 13, the extension end 22 on the bracket 2 and the hair roller 3 are lifted, a certain distance space is formed between the extension end and the material receiving platform 5, and an operator can conveniently adhere and slice with a glass slide.
The ultrathin section cutting device is installed and used when an ultrathin section is cut, is convenient to install and operate, does not need a user to hold a writing brush to stretch and sweep the wound section, and is high in section success rate.
Claims (6)
1. A sample sectioning assistance device for an electron microscope, characterized in that: it comprises a base, a bracket, a hair roller and a pressure lever; an upper sliding rail and a lower sliding rail are arranged on the right side of the base, the left side of the support is slidably arranged in the upper sliding rail and the lower sliding rail, and a locking mechanism is arranged between the upper sliding rail and the support and between the upper sliding rail and the lower sliding rail and the support; a transverse extending end is respectively arranged at the front end and the rear end of the right side of the bracket, two ends of the hair roller are arranged at the inner side of the extending end in a sliding manner through strip-shaped sliding blocks, and a spring is arranged between the extending end and the strip-shaped sliding blocks; a reset torsion spring is connected between the tail end of the hair roller and the strip-shaped sliding block; a second extending end which is inclined towards the lower left side is arranged at the right end of each extending end, the front end and the rear end of the pressure lever can be respectively arranged on the inner side corresponding to the second extending end in a vertically sliding manner, and a second spring is arranged between the pressure lever and the second extending end; a wiring hole for connecting the left end of the hair roller slide rail and the top end of the pressure rod slide rail is arranged in each of the extension end and the second extension end, a pull wire is arranged in the wiring hole, one end of the pull wire is fixed and wound at one end of the hair roller, and the other end of the pull wire is fixedly connected with the pressure rod; the base is provided with a fixing mechanism.
2. A sample sectioning assistance device for an electron microscope as claimed in claim 1, wherein: an adjusting bolt is rotatably arranged on the right side of the pressure lever.
3. A specimen slice assist device for an electron microscope as claimed in claim 1 wherein: the fixing mechanism is a manual sucker.
4. A sample sectioning assistance device for an electron microscope as claimed in claim 1, wherein: and a plurality of pulleys matched with the pull wires are arranged in the wire feeding holes.
5. A sample sectioning assistance device for an electron microscope as claimed in claim 1, wherein: locking mechanism includes magnet I and the magnet II with I complex of magnet on the support on upper and lower slide rail top.
6. A specimen slice assist device for an electron microscope as claimed in claim 1 wherein: the hair roller is a metal roller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211307385.7A CN115791253A (en) | 2022-10-25 | 2022-10-25 | Sample slice auxiliary device for electron microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211307385.7A CN115791253A (en) | 2022-10-25 | 2022-10-25 | Sample slice auxiliary device for electron microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115791253A true CN115791253A (en) | 2023-03-14 |
Family
ID=85433673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211307385.7A Withdrawn CN115791253A (en) | 2022-10-25 | 2022-10-25 | Sample slice auxiliary device for electron microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115791253A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117139513A (en) * | 2023-09-04 | 2023-12-01 | 乐宏线缆有限公司 | Cable slicing device |
-
2022
- 2022-10-25 CN CN202211307385.7A patent/CN115791253A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117139513A (en) * | 2023-09-04 | 2023-12-01 | 乐宏线缆有限公司 | Cable slicing device |
| CN117139513B (en) * | 2023-09-04 | 2024-04-05 | 乐宏线缆有限公司 | Cable slicing device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20230314 |