CN115786116A

CN115786116A - Culture apparatus and reactor for tissue engineering culture

Info

Publication number: CN115786116A
Application number: CN202310024150.5A
Authority: CN
Inventors: 林展翼; 梅静怡; 周浩浩; 周嘉辉
Original assignee: Ji Hua Laboratory
Current assignee: Ji Hua Laboratory
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2023-03-14

Abstract

The application relates to tissue engineering culture technical field, specifically provides a tissue engineering cultivates and uses culture apparatus and reactor, and the device includes: the incubator body comprises a seed cell culture tube; the two support material fixing components are symmetrically arranged on the seed cell culture tube and comprise a shrinking claw and a thread outer sleeve, the shrinking claw is in sliding connection with the seed cell culture tube, the thread outer sleeve is sleeved outside the shrinking claw and is in threaded connection with the shrinking claw, one end, close to the middle of the seed cell culture tube, of the shrinking claw is provided with a plurality of finger parts arranged at intervals, a pipe orifice, close to the middle of the seed cell culture tube, of the thread outer sleeve is smaller than the outer diameter of the shrinking claw, and the thread outer sleeve is matched with the shrinking claw to enable the finger parts to be folded so as to fix the shrinking claw and the end part of the support material on the seed cell culture tube; the device can prevent the displacement of the support material to ensure that stress stimulation can be applied to the seed cells, thereby effectively improving the culture effect of the seed cells.

Description

Culture apparatus and reactor for tissue engineering culture

Technical Field

The application relates to the technical field of tissue engineering culture, in particular to a culture device and a reactor for tissue engineering culture.

Background

The prior art cultivates seed cell in culture apparatus for tissue engineering culture, and current culture apparatus for tissue engineering culture includes the culture apparatus, is equipped with the silica gel hose in the culture apparatus, has cup jointed the support material that is used for inoculating seed cell on the silica gel hose. In the process of culturing the seed cells, mechanical stimulation needs to be applied to the seed cells, the mechanical stimulation easily causes the displacement of a support material sleeved on a silica gel hose, so that the concentration distribution of extracellular matrix secreted by the seed cells is uneven, and the culture effect of the seed cells is positively correlated with the uniformity of the concentration distribution of the extracellular matrix, while the existing culture device for tissue engineering culture has the condition of uneven concentration distribution of the extracellular matrix, so that the existing culture device for tissue engineering culture has poor effect of culturing the seed cells.

In view of the above problems, no effective technical solution exists at present.

Disclosure of Invention

An object of the present application is to provide a culture apparatus and a reactor for tissue engineering culture, which can prevent the displacement of a scaffold material during the culture process, thereby effectively improving the culture effect of seed cells.

In a first aspect, the present application provides a culture device for tissue engineering culture, for culturing seed cells, comprising:

the incubator body comprises a seed cell culture tube, wherein a bracket material for inoculating seed cells is sleeved on one side of the seed cell culture tube close to a culture medium;

two fixed subassemblies of support material, the symmetry sets up on seed cell culture pipe, it is including crimpling claw and screw thread overcoat, crimpling claw and seed cell culture pipe sliding connection, the screw thread overcoat cup joints outside crimpling claw and with crimpling claw threaded connection, the one end that crimpling claw is close to seed cell culture pipe middle part has the finger portion of a plurality of intervals settings, the mouth of pipe of the one end that the screw thread overcoat is close to seed cell culture pipe middle part is less than crimpling claw's external diameter, the screw thread overcoat makes finger portion draw in order to fix crimpling claw and support material tip on seed cell culture pipe with crimpling claw cooperation.

The utility model provides a pair of culture apparatus is used in tissue engineering cultivation, cup joint the screw thread overcoat outside the shrink claw and make screw thread overcoat and shrink claw threaded connection, because the mouth of pipe that the screw thread overcoat is close to the one end at seed cell culture pipe middle part is less than the external diameter of shrink claw, consequently, can make a plurality of finger portions of shrink claw draw in through the mode of screwing the screw thread overcoat, thereby realize shrinking claw and support material end fixing on seed cell culture pipe, because support material can not take place to shift at the culture in-process, consequently this culture apparatus can avoid appearing effectively because support material shifts and leads to the inhomogeneous condition of concentration distribution of the extracellular matrix of seed cell secretion, thereby improve the culture effect of seed cell effectively.

Optionally, the seed cell culture tube comprises a first hard hollow tube, a second hard hollow tube and an elastic hose, two ends of the elastic hose are respectively connected with the first hard hollow tube and the second hard hollow tube, and the support material is sleeved outside the elastic hose.

Optionally, both ends of the elastic hose are provided with annular protrusions for limiting the mounting position of the bracket material.

After the support material is sleeved with the circular-ring-shaped bulges, the two ends of the support material are in interference fit with the circular-ring-shaped bulges, and the circular-ring-shaped bulges play a role in limiting the installation position of the support material, so that the situation that the support material is displaced when the tightening claw slides can not occur, and the convenience of moving the finger parts of the tightening claw to the end part of the support material is effectively improved.

Optionally, the peripheries of the ends of the first hard hollow tube and the second hard hollow tube close to the elastic hose are both provided with barbs, and two ends of the elastic hose are respectively in interference fit with the barbs of the first hard hollow tube and the barbs of the second hard hollow tube.

Elastic hose cup joints on the barb of first stereoplasm hollow tube and second stereoplasm hollow tube, because elastic hose's both ends all with barb interference fit, consequently this technical scheme's barb can play the effect that the antiskid is taken off to elastic hose, and this technical scheme sets up the mode that sets up to the barb through the tip periphery with first stereoplasm hollow tube and second stereoplasm hollow tube near elastic hose and reduces elastic hose effectively and drops the possibility to improve stereoplasm hollow tube and elastic hose's leakproofness effectively.

Optionally, the incubator body further comprises an incubator housing, a first metal plug and a second metal plug, the incubator housing comprises a glass tube, the glass tube is located in the middle of the incubator housing, and two ends of the incubator housing are respectively in threaded connection with the first metal plug and the second metal plug so that two ends of the glass tube are respectively tightly attached to the first metal plug and the second metal plug.

Optionally, the culture device for tissue engineering culture further comprises a first end cap and a second end cap, the first end cap is located outside the first metal plug, and the second end cap is located outside the second metal plug.

Optionally, the incubator enclosure, the first metal plug and the glass tube form a first sealed cavity, the incubator enclosure, the second metal plug and the glass tube form a second sealed cavity, and the first sealed cavity and the second sealed cavity are both provided with latex rings.

After screwing the first metal plug and the second metal plug, the latex ring in the first sealing cavity is extruded to seal the first metal plug and the glass tube, and the latex ring in the second sealing cavity is extruded to seal the second metal plug and the glass tube, so that the culture medium is further prevented from being leaked from a gap between the first metal plug and the glass tube and a gap between the second metal plug and the glass tube.

Optionally, both ends of the glass tube are provided with sealing rings.

In the process of screwing the first metal plug and the second metal plug, the sealing ring can disperse acting forces applied by the first metal plug and the second metal plug, so that the technical scheme can effectively reduce the situation that the glass tube is cracked due to overlarge acting forces applied to the glass tube by the first metal plug and the second metal plug.

Optionally, the composition of the scaffold material is one or more of polylactic acid, polybutylene succinate-co-adipate, polybutylene terephthalate-co-adipate, polyhydroxyalkanoates, polypropylene carbonate, polyglycolic acid, polycaprolactone, polyglycerol sebacate, a first polymer comprising 84% glycolide and 16% trimethylene carbonate, a second polymer comprising 84% glycolide, 14% tmc and 2% polyethylene succinate, and a third polymer comprising 87% glycolide, 7% tmc and 6% polyethylene glycol.

In a second aspect, the present application also provides a reactor comprising the culture device for tissue engineering culture as provided in the first aspect above.

The utility model provides a pair of reactor, cup joint the screw thread overcoat outside the shrink claw and make screw thread overcoat and shrink claw threaded connection, because the mouth of pipe of the screw thread overcoat near the one end at seed cell culture pipe middle part is less than the external diameter of shrink claw, consequently, can make a plurality of fingers of shrink claw draw in through the mode of screwing the screw thread overcoat, thereby realize shrinking claw and support material end fixing on seed cell culture pipe, because support material can not take place to shift at the culture in-process, consequently this reactor can avoid appearing effectively because support material shifts and lead to the inhomogeneous condition of concentration distribution of the secreted extracellular matrix of seed cell, thereby improve the culture effect of seed cell effectively.

By last knowing, the utility model provides a culture apparatus and reactor are used in tissue engineering cultivation, cup joint the screw thread overcoat outside the shrink claw and make screw thread overcoat and shrink claw threaded connection, because the mouth of pipe of the screw thread overcoat near the one end at seed cell culture pipe middle part is less than the external diameter of shrink claw, consequently can make a plurality of fingers portions of shrink claw draw in through the mode of the screw thread overcoat of screwing, thereby realize shrink claw and support material end fixing on seed cell culture pipe, because the support material can not take place to shift in the process of cultivateing, consequently this culture apparatus can avoid appearing effectively because support material shifts and leads to the inhomogeneous condition of concentration distribution of the secreted extracellular matrix of seed cell, thereby improve the culture effect of seed cell effectively.

Drawings

FIG. 1 is a schematic view of a tissue culture apparatus according to an embodiment of the present invention.

FIG. 2 is a schematic diagram of a seed cell culture tube and scaffold material fixation assembly according to an embodiment of the present disclosure.

FIG. 3 is a schematic view showing the structure of a seed cell culture tube according to an embodiment of the present invention.

Fig. 4 is a schematic structural diagram of a rigid hollow tube according to an embodiment of the present disclosure.

Fig. 5 is a schematic structural diagram of the screw housing after being screwed according to the embodiment of the present application.

Fig. 6 is a schematic structural diagram of a thread jacket according to an embodiment of the present application.

Fig. 7 is a schematic structural view of a tightening claw provided in an embodiment of the present application.

Reference numerals: 1. an incubator body; 11. an incubator housing; 12. a glass tube; 13. a seed cell culture tube; 131. a first rigid hollow tube; 132. a second rigid hollow tube; 133. an elastic hose; 1331. a circular bulge; 14. a first metal plug; 15. a second metal plug; 2. a stent material securing assembly; 21. a jaw is contracted; 22. a threaded outer sleeve; 3. a medium inflow tube; 4. a culture medium outflow pipe; 5. a latex ring; 6. a first end cap; 7. a second end cap.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, as presented in the figures, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first" and "second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; may be mechanically, electrically or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature "on," "above" and "over" the second feature may include the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed.

In a first aspect, as shown in fig. 1 to 7, the present application provides a culture device for tissue engineering culture, for culturing seed cells, comprising:

the incubator body 1 comprises a seed cell culture tube 13, and a bracket material for inoculating seed cells is sleeved on one side of the seed cell culture tube 13 close to a culture medium;

two fixed subassemblies of support material 2, the symmetry sets up on seed cell culture pipe 13, it is including tightening claw 21 and screw thread overcoat 22, tightening claw 21 and seed cell culture pipe 13 sliding connection, screw thread overcoat 22 cup joints outside tightening claw 21 and with tightening claw 21 threaded connection, the one end that tightening claw 21 is close to seed cell culture pipe 13 middle part has the finger portion of a plurality of intervals settings, the mouth of pipe of the screw thread overcoat 22 one end that is close to seed cell culture pipe 13 middle part is less than the external diameter of tightening claw 21, screw thread overcoat 22 makes finger portion draw in order to fix tightening claw 21 and support material tip on seed cell culture pipe 13 with tightening claw 21 and the cooperation of tightening claw 21.

The incubator body 1 is an existing incubator for tissue engineering culture, the incubator body 1 comprises a seed cell culture tube 13, a culture medium for providing nutrition for seed cells is filled in the seed cell culture tube 13, and the seed cells can be any one or more of cells such as human smooth muscle cells, human fibroblasts, human endothelial cells, bovine smooth muscle cells, bovine fibroblasts and the like. The side of the seed cell culture tube 13 close to the culture medium is sleeved with a scaffold material for inoculating seed cells, and the scaffold material has good cell compatibility, so that the seed cells can grow and proliferate on the scaffold material, and the embodiment is equivalent to culturing the seed cells outside the seed cell culture tube 13. The quantity of the fixed subassembly 2 of support material is two, two fixed subassemblies 2 of support material use the center of seed cell culture pipe 13 to set up on the outside of seed cell culture pipe 13 as the symmetry center symmetry, each fixed subassembly 2 of support material all includes tightening claw 21 and screw thread overcoat 22, tightening claw 21 can slide for seed cell culture pipe 13, the one end that tightening claw 21 is close to seed cell culture pipe 13 middle part has the finger portion of a plurality of intervals settings, the interval between two adjacent finger portions is the same, the mouth of pipe that screw thread overcoat 22 is close to the one end in seed cell culture pipe 13 middle part is less than the external diameter of tightening claw 21, screw thread overcoat 22 cup joints outside tightening claw 21 and with tightening claw 21 threaded connection, specifically, tightening claw 21 has the external screw thread towards the one end of keeping away from seed cell culture pipe 13 middle part, screw thread overcoat 22 has the internal thread towards the one end of keeping away from seed cell culture pipe 13 middle part. It should be understood that fig. 1 is mainly used for illustrating the connection relationship of the incubator body 1, the first end cap 6 and the second end cap 7, and therefore fig. 1 is a simplified illustration of the specific structure of the support material fixing assembly 2 and the seed cell culture tube 13, and the specific structure of the support material fixing assembly 2 and the seed cell culture tube 13 is shown in fig. 2 to 7.

The working principle of the embodiment is as follows: the screw thread outer sleeve 22 is sleeved outside the tightening claw 21 and is in threaded connection with the tightening claw 21, and the pipe orifice of one end, close to the middle part of the seed cell culture tube 13, of the screw thread outer sleeve 22 is smaller than the outer diameter of the tightening claw 21, so that in the process of screwing the screw thread outer sleeve 22, the pipe orifice of one end, close to the middle part of the seed cell culture tube 13, of the screw thread outer sleeve 22 is in contact with the finger parts of the tightening claw 21 and enables the finger parts of the tightening claw 21 to be folded, namely the device can enable the finger parts of the tightening claw 21 to be folded in a mode of screwing the screw thread outer sleeve 22, and accordingly the tightening claw 21 and the end part of the support material are fixed on the seed cell culture tube 13. It will be appreciated that the threaded outer sleeve 22 and the tightening claws 21 can be moved relative to the seed cell culture tube 13 and the scaffold material before the threaded outer sleeve 22 is tightened (i.e., the plurality of fingers of the tightening claws 21 are collapsed), and thus the positions of the threaded outer sleeve 22 and the tightening claws 21 can be adjusted by sliding the tightening claws 21 to move the fingers of the tightening claws 21 to the ends of the scaffold material before the threaded outer sleeve 22 is tightened. In addition, since the scaffold material is not displaced during the culture process, the embodiment can ensure that the stress stimulation can be applied to the seed cells, thereby further improving the culture effect of the seed cells.

The utility model provides a pair of culture apparatus for tissue engineering is cultivateed, cup joint screw thread overcoat 22 outside tightening claw 21 and make screw thread overcoat 22 and tightening claw 21 threaded connection, because the mouth of pipe of screw thread overcoat 22 near the one end at seed cell culture pipe 13 middle part is less than the external diameter of tightening claw 21, consequently can make a plurality of fingers portions of tightening claw 21 draw in through the mode of screwing screw thread overcoat 22, thereby realize fixing tightening claw 21 and support material tip on seed cell culture pipe 13, because the support material can not take place the aversion in the culture process, consequently this culture apparatus can avoid effectively appearing leading to the inhomogeneous condition of concentration distribution of the extracellular matrix of seed cell secretion because the support material aversion, thereby improve seed cell's culture effect effectively.

In some embodiments, both ends of the seed cell culture tube 13 are connected to a buffer solution supply assembly (not shown) for supplying buffer solution to the circulation of the seed cell culture tube 13. The buffer may be a PBS buffer that flows inside the seed cell culture tube 13 to provide pulsatile tension to the seed cell culture tube 13. Specifically, the buffer solution provides the subassembly and includes the buffer solution inflow tube, the buffer solution outflow tube, peristaltic pump and buffer solution stock solution bottle, the both ends of seed cell culture pipe 13 are connected with the buffer solution stock solution bottle through buffer solution inflow tube and buffer solution outflow tube respectively, the peristaltic pump sets up on the buffer solution inflow tube, the peristaltic pump is arranged in driving the buffer solution in the buffer solution stock solution bottle and flows towards seed cell culture pipe 13 unilaterally, buffer solution inflow tube and buffer solution outflow tube can be the pipeline that can carry liquid such as rubber tube or silicon hose, the peristaltic pump is prior art, the detailed discussion will not be repeated here.

In some embodiments, the end of the threaded outer sleeve 22 near the middle of the seed cell culture tube 13 is stepped, spherical, conical, or ellipsoidal. The end of the tightening claw 21 close to the middle of the seed cell culture tube 13 is also stepped, spherical, conical or ellipsoidal, and in this embodiment, the end of the threaded outer sleeve 22 close to the middle of the seed cell culture tube 13 is preferably ellipsoidal to reduce the interference of the threaded outer sleeve 22 on the flow of the culture medium as much as possible, thereby avoiding the situation that the growth rate of the seed cells close to the threaded outer sleeve 22 is different from the growth rate of the seed cells far from the threaded outer sleeve 22 as much as possible and further effectively improving the uniformity of the growth of the seed cells.

In some embodiments, the seed cell culture tube 13 includes a first hard hollow tube 131, a second hard hollow tube 132, and an elastic hose 133, two ends of the elastic hose 133 are respectively connected to the first hard hollow tube 131 and the second hard hollow tube 132, and the bracket material is sleeved on the outer side (i.e., the side close to the culture medium) of the elastic hose 133. The first hard hollow tube 131 and the second hard hollow tube 132 may be BPT (high polymer material) hard tubes, the structures of the first hard hollow tube 131 and the second hard hollow tube 132 are all as shown in fig. 4, the elastic hose 133 may be any one of rubber hoses, silica gel hoses, or resin hoses, two ends of the elastic hose 133 are respectively sleeved on the outer side of the first hard hollow tube 131 and the outer side of the second hard hollow tube 132, and the bracket material is sleeved on the outer side of the elastic hose 133. It should be understood that the buffer liquid of the above embodiment flows inside the first rigid hollow tube 131, the second rigid hollow tube 132 and the elastic hose 133.

The above embodiment can adjust the position of the finger by sliding the tightening claws 21 before screwing the screw jacket 22, but when sliding the tightening claws 21, the tightening claws 21 may move the bracket material together, thereby making it difficult for the finger of the tightening claw 21 to move to the end of the bracket material.

In some embodiments, both ends of the elastic hose 133 are provided with annular protrusions 1331 for defining the mounting position of the stent material. The annular protrusions 1331 are arranged at the two ends of the elastic hose 133, before the tightening claw 21 slides, the bracket material is sleeved on the annular protrusions 1331 in the embodiment, the diameter of the annular protrusions 1331 is larger than that of the bracket material, so that after the bracket material is sleeved on the annular protrusions 1331, the two ends of the bracket material are in interference fit with the annular protrusions 1331, and the annular protrusions 1331 play a role in limiting the installation position of the bracket material, so that the bracket material cannot be displaced when the tightening claw 21 slides, and the convenience of moving the finger parts of the tightening claw 21 to the end part of the bracket material is effectively improved. In addition, during the seed cell culture process, the annular protrusion 1331 can fix the scaffold material, so this embodiment can further reduce the possibility of displacement of the scaffold material during the seed cell culture process. In some preferred embodiments, after the screw housing 22 is screwed, the finger portion of the tightening claw 21 abuts against the annular protrusion 1331, which can avoid the situation that the finger portion of the tightening claw 21 can not fix the stent material after being folded due to the small size of the stent material.

The elastic tube 133 of the above embodiment is connected to the first rigid hollow tube 131 and the second rigid hollow tube 132 in a sleeved manner, and when a mechanical stimulus is applied to the seed cells, the elastic tube 133 may fall off, and at this time, the buffer solution in the first rigid hollow tube 131, the second rigid hollow tube 132, and the elastic tube 133 leaks into the culture medium in the incubator body 1 to contaminate the culture medium.

In some embodiments, the outer peripheries of the ends of the first rigid hollow tube 131 and the second rigid hollow tube 132 near the elastic hose 133 are each provided with barbs, and both ends of the elastic hose 133 are interference fitted with the barbs of the first rigid hollow tube 131 and the second rigid hollow tube 132, respectively. The peripheries of the ends of the first hard hollow tube 131 and the second hard hollow tube 132 close to the elastic hose 133 are respectively provided with a barb, the barbs comprise at least one truncated cone structure, the diameter of one side of the truncated cone structure close to the elastic hose 133 is larger than that of one side of the truncated cone structure away from the elastic hose 133, the elastic hose 133 is sleeved on the barbs of the first hard hollow tube 131 and the second hard hollow tube 132, and both ends of the elastic hose 133 are in interference fit with the barbs, so that the barbs of the embodiment can play a role in preventing the elastic hose 133 from slipping off, that is, the embodiment effectively reduces the possibility that the elastic hose 133 slips off in a mode that the peripheries of the ends of the first hard hollow tube 131 and the second hard hollow tube 132 close to the elastic hose 133 are provided with the barbs, thereby avoiding the situation that buffer solutions in the first hard hollow tube 131, the second hard hollow tube 132 and the elastic hose 133 can leak into the culture medium in the incubator body 1 to pollute the culture medium as far as possible. It should be understood that, in order to improve the anti-slip function of the barb, the barb includes a plurality of circular truncated cone structures connected in sequence, the elastic hose 133 is sleeved on the circular truncated cone structure with the maximum distance from the center of the incubator body 1, even if the elastic hose 133 slips from the circular truncated cone structure with the maximum distance from the center of the incubator body 1, other circular truncated cone structures can still play the anti-slip function on the elastic hose 133, for example, the barb includes a first circular truncated cone structure, a second circular truncated cone structure and a third circular truncated cone structure connected in sequence, the distance from the first circular truncated cone structure to the center of the incubator body 1 is maximum, the elastic hose 133 is sleeved on the first circular truncated cone structure, even if the elastic hose 133 slips from the first circular truncated cone structure, the second circular truncated cone structure and the third circular truncated cone structure can still play the anti-slip function on the elastic hose 133.

In some embodiments, the incubator body 1 further includes an incubator housing 11, a first metal plug 14, and a second metal plug 15, the incubator housing 11 includes a glass tube 12, the glass tube 12 is located in the middle of the incubator housing 11, the glass tube 12 is fixedly connected to the incubator housing 11, and two ends of the incubator housing 11 are respectively connected to the first metal plug 14 and the second metal plug 15 by screw threads, so that two ends of the glass tube 12 are respectively attached to the first metal plug 14 and the second metal plug 15. Specifically, this embodiment connects both ends of the glass tube 12 to the first metal plug 14 and the second metal plug 15 by screwing the first metal plug 14 and the second metal plug 15 so that both ends of the glass tube 12 are in contact with the first metal plug 14 and the second metal plug 15, respectively. The first hard hollow tube 131 penetrates through the first metal plug 14, the second hard hollow tube 132 penetrates through the second metal plug 15, the first metal plug 14 is connected with the culture medium inflow tube 3, and the second metal plug 15 is connected with the culture medium outflow tube 4.

The above-described embodiment brings both ends of the glass tube 12 into contact with the first metal plugs 14 and the second metal plugs 15 by screwing the first metal plugs 14 and the second metal plugs 15, and there may occur a case where the glass tube 12 is broken due to an excessive force applied to the glass tube 12 by the first metal plugs 14 and the second metal plugs 15 during screwing of the first metal plugs 14 and the second metal plugs 15.

To solve this problem, in some embodiments, the glass tube 12 is provided with sealing rings at both ends. The sealing ring may be a rubber ring, a silicone ring, or the like, which can disperse the force applied by the first metal plug 14 and the second metal plug 15, and during screwing the first metal plug 14 and the second metal plug 15, since the sealing ring can disperse the force applied by the first metal plug 14 and the second metal plug 15, this embodiment can effectively reduce the occurrence of the situation that the glass tube 12 is cracked due to the excessive force applied to the glass tube 12 by the first metal plug 14 and the second metal plug 15. In addition, after the first metal plug 14 and the second metal plug 15 are screwed, the sealing rings at the two ends of the glass tube 12 are pressed, and at this time, the culture medium is not easy to leak through the gap between the first metal plug 14 and the glass tube 12 and the gap between the second metal plug 15 and the glass tube 12, so that the sealing performance between the first metal plug 14 and the glass tube 12 and the sealing performance between the second metal plug 15 and the glass tube 12 are effectively improved.

In some embodiments, the culture device for tissue engineering culture further comprises a first end cap 6 and a second end cap 7, wherein the first end cap 6 is positioned outside the first metal plug 14, and the second end cap 7 is positioned outside the second metal plug 15. In this example, one end of the first hard hollow tube 131 far from the elastic hose 133 is connected with the first end cap 6 in a threaded manner, one end of the second hard hollow tube 132 far from the elastic hose 133 is connected with the second end cap 7 in a threaded manner, specifically, one end of the first hard hollow tube 131 far from the elastic hose 133 is provided with an external thread, the first end cap 6 is provided with an internal thread, one end of the second hard hollow tube 132 far from the elastic hose 133 is provided with an external thread, and the second end cap 7 is provided with an internal thread. This embodiment allows the first rigid hollow tube 131 to be secured to the first metal plug 14 by screwing the first end cap 6 and the second rigid hollow tube 132 to be secured to the second metal plug 15 by screwing the second end cap 7.

It should be understood that the assembly process of the culture device for tissue engineering culture of the embodiment of the present application is as follows: 1. sleeving the bracket material on the annular bulge 1331 of the elastic hose 133; 2. sliding the tightening claws 21 to move the finger portions of the tightening claws 21 to the end of the holder material; 3. tightening the threaded sleeve 22 to secure the tightening claws 21 and the bracket material to the elastic hose 133; 4. two ends of the elastic hose 133 are respectively sleeved on the barbs of the first hard hollow tube 131 and the second hard hollow tube 132; 5. placing the first rigid hollow tube 131, the second rigid control tube 132 and the elastic hose inside the glass tube 12, and passing the first rigid hollow tube 131 through the first metal plug 14 and the second rigid hollow tube 132 through the second metal plug 15; 6. screwing the first metal plug 14 and the second metal plug 15; 7. the first end cap 6 and the second end cap 7 are screwed.

In some embodiments, the incubator enclosure 11, the first metal plug 14, and the glass tube 12 form a first sealed chamber, the incubator enclosure 11, the second metal plug 15, and the glass tube form a second sealed chamber, and the latex rings 5 are disposed in both the first sealed chamber and the second sealed chamber. In this embodiment, the latex rings 5 are disposed in the first sealing cavity and the second sealing cavity, when the first metal plug 14 and the second metal plug 15 are screwed, the latex ring 5 in the first sealing cavity is pressed to seal the first metal plug 14 and the glass tube 12, and the latex ring 5 in the second sealing cavity is pressed to seal the second metal plug 15 and the glass tube 12, so as to further prevent the culture medium from leaking from the gap between the first metal plug 14 and the glass tube 12 and the gap between the second metal plug 15 and the glass tube 12.

In some embodiments, the composition of the stent material is one or more of polylactic acid, polybutylene succinate-co-adipate, polybutylene terephthalate-co-adipate, polyhydroxyalkanoate, polypropylene carbonate, polyglycolic acid, polycaprolactone, polyglycerol sebacate, a first polymer comprising 84% glycolide and 16% trimethylene carbonate, a second polymer comprising 84% glycolide, 14% tmc (transition metal carbide) and 2% polyethylene succinate, and a third polymer comprising 87% glycolide, 7% tmc and 6% polyethylene glycol.

Therefore, the culture device for tissue engineering culture provided by the application can effectively avoid the condition that the concentration distribution of extracellular matrix secreted by seed cells is uneven due to the displacement of the support material, and effectively improve the culture effect of the seed cells.

The working principle of a reactor provided by the embodiments of the present application is the same as that of the culture device for tissue engineering culture provided by the first aspect, and will not be discussed in detail here.

Therefore, the culture device and the reactor for tissue engineering culture have the advantages that the thread outer sleeve 22 is sleeved outside the tightening claw 21, the thread outer sleeve 22 is in threaded connection with the tightening claw 21, and the pipe orifice of one end, close to the middle part of the seed cell culture tube 13, of the thread outer sleeve 22 is smaller than the outer diameter of the tightening claw 21, so that the finger parts of the tightening claw 21 can be gathered in a mode of screwing the thread outer sleeve 22, the end part of the support material is fixed on the seed cell culture tube 13, and the tightening claw 21 and the support material cannot shift in the culture process, so that the condition that the concentration distribution of extracellular matrixes secreted by seed cells is uneven due to the shift of the support material can be effectively avoided, and the culture effect of the seed cells is effectively improved.

In the embodiments provided in the present application, it should be understood that the disclosed apparatus may be implemented in other ways. The above-described device embodiments are merely illustrative, and for example, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some communication interfaces, devices or units, and may be in an electrical, mechanical or other form.

In this document, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions.

The above description is only an example of the present application and is not intended to limit the scope of the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A culture apparatus for tissue engineering culture for culturing seed cells, comprising:

the incubator body comprises a seed cell culture tube, wherein a bracket material for inoculating the seed cells is sleeved on one side of the seed cell culture tube close to a culture medium;

two fixed subassemblies of support material, the symmetry sets up on the seed cell culture pipe, it includes crimple claw and screw thread overcoat, crimple claw with seed cell culture pipe sliding connection, the screw thread overcoat cup joints crimple claw outer and with crimple claw threaded connection, crimple claw is close to the one end at seed cell culture pipe middle part has the finger portion that a plurality of intervals set up, the screw thread overcoat is close to the mouth of pipe of the one end at seed cell culture pipe middle part is less than crimple claw's external diameter, the screw thread overcoat with crimple claw cooperation makes finger portion draws in order will crimple claw with the support material end fixing on the seed cell culture pipe.

2. The tissue engineering culture device according to claim 1, wherein the seed cell culture tube comprises a first rigid hollow tube, a second rigid hollow tube and an elastic hose, two ends of the elastic hose are respectively connected with the first rigid hollow tube and the second rigid hollow tube, and the scaffold material is sleeved outside the elastic hose.

3. The culture apparatus for tissue engineering culture according to claim 2, wherein annular protrusions for limiting the mounting position of the scaffold material are provided at both ends of the elastic tube.

4. The culture device for tissue engineering culture according to claim 2, wherein the first hard hollow tube and the second hard hollow tube each have a barb on the outer periphery of the end portion close to the elastic hose, and both ends of the elastic hose are in interference fit with the barbs of the first hard hollow tube and the barbs of the second hard hollow tube, respectively.

5. The culture device for tissue engineering culture according to claim 1, wherein the incubator body further comprises an incubator housing, a first metal plug and a second metal plug, the incubator housing comprises a glass tube, the glass tube is located in the middle of the incubator housing, and two ends of the incubator housing are respectively in threaded connection with the first metal plug and the second metal plug so that two ends of the glass tube are respectively tightly attached to the first metal plug and the second metal plug.

6. The tissue engineering culture device of claim 5, further comprising a first end cap and a second end cap, wherein the first end cap is located outside the first metal plug and the second end cap is located outside the second metal plug.

7. The tissue-engineering-culture device according to claim 5, wherein the incubator enclosure, the first metal plug, and the glass tube form a first sealed cavity, the incubator enclosure, the second metal plug, and the glass tube form a second sealed cavity, and latex rings are disposed in the first sealed cavity and the second sealed cavity.

8. The culture device for tissue engineering culture according to claim 5, wherein both ends of the glass tube are provided with a sealing ring.

9. The culture device for tissue engineering culture according to claim 1, wherein the scaffold material is composed of one or more of polylactic acid, polybutylene succinate-co-adipate, polybutylene terephthalate-co-adipate, polyhydroxyalkanoate, polypropylene carbonate, polyglycolic acid, polycaprolactone, polyglycerol sebacate, a first polymer comprising 84% glycolide and 16% trimethylene carbonate, a second polymer comprising 84% glycolide, 14% TMC and 2% polyethylene succinate, and a third polymer comprising 87% glycolide, 7% TMC and 6% polyethylene glycol.

10. A reactor comprising the culture apparatus for tissue engineering culture according to any one of claims 1 to 9.