CN115786079A

CN115786079A - Biological reaction bag and bioreactor

Info

Publication number: CN115786079A
Application number: CN202211734065.XA
Authority: CN
Inventors: 刘金林; 李志安; 聂江龙; 张奕杰; 史振志
Original assignee: Guangzhou Aibeitai Biotechnology Co ltd
Current assignee: Guangzhou Aibeitai Biotechnology Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-03-14
Anticipated expiration: 2042-12-30
Also published as: CN115786079B

Abstract

The invention relates to a biological reaction bag and a bioreactor, wherein the biological reaction bag comprises: a bag body; the first connecting structure and the second connecting structure are respectively used for detachably connecting two opposite ends of the bioreactor along a first direction; one end of the telescopic stirring shaft is detachably connected with the first connecting structure, and the other end of the telescopic stirring shaft is detachably connected with the second connecting structure; the size of the telescopic stirring shaft along the first direction is adjustable; the bioreactor comprises a motor and a first connecting piece connected with the motor, the first connecting structure comprises a second connecting piece detachably connected with the first connecting piece, and the first connecting piece and the second connecting piece are configured to enable the motor and the telescopic stirring shaft to be coaxially arranged. The biological reaction bag is convenient to disassemble and assemble by arranging the first connecting structure and the second connecting structure; through setting up flexible (mixing) shaft, flexible (mixing) shaft is in the shrink state before bioreactor is installed to the biological reaction bag, is convenient for transport.

Description

Biological reaction bag and bioreactor

Technical Field

The invention relates to the technical field of bioreactors, in particular to a bioreactor bag and a bioreactor.

Background

When an experiment is performed in a bioreactor, a disposable bioreactor bag for the experiment is usually installed in the bioreactor, and a new bioreactor bag needs to be replaced each time the experiment is performed.

However, since the motor for driving the rotation of the stirring shaft in the bioreactor is fixed on the rigid support shell of the bioreactor, the bioreactor is difficult to disassemble when the bioreactor is replaced, and the problem that the stirring shaft of the motor and the stirring shaft of the new bioreactor are not coaxial is easily caused, which affects the stirring effect.

Disclosure of Invention

Therefore, it is necessary to provide a bioreactor and a bioreactor, so as to facilitate the disassembly and assembly of the bioreactor, improve the efficiency of replacing the bioreactor, and enable the motor and the stirring shaft to be coaxially arranged.

According to one aspect of the present application, there is provided a bioreactor bag for a bioreactor, the bioreactor bag comprising:

a bag body having a first receiving cavity;

the bioreactor comprises a first connecting structure and a second connecting structure which are sequentially arranged along a first direction, wherein the first connecting structure and the second connecting structure are respectively used for detachably connecting two opposite ends of the bioreactor along the first direction; and

one end of the telescopic stirring shaft is detachably connected with the first connecting structure, the other end of the telescopic stirring shaft is detachably connected with the second connecting structure, and the telescopic stirring shaft is accommodated in the first accommodating cavity; the size of the telescopic stirring shaft along the first direction is adjustable;

wherein, bioreactor includes the motor and connects the first connecting piece of motor, first connecting structure include with first connecting piece can dismantle the second connecting piece that connects, flexible (mixing) shaft with the coaxial setting of second connecting piece, first connecting piece with the second connecting piece is configured to make the motor with flexible (mixing) shaft is coaxial to be set up.

According to the biological reaction bag, the first connecting structure and the second connecting structure are arranged, and the first connecting structure and the second connecting structure are detachably connected with the bioreactor, so that the biological reaction bag can be conveniently disassembled and assembled when being replaced, and the disassembling and assembling time is saved; the telescopic stirring shaft is arranged and is in a contracted state before the biological reaction bag is arranged in the bioreactor, so that the biological reaction bag is convenient to transport; through setting up first connecting piece and second connecting piece to make the motor and the coaxial setting of flexible (mixing) shaft, avoid influencing the stirring effect of flexible (mixing) shaft.

In one embodiment, the telescopic stirring shaft comprises a first shaft and a second shaft, wherein the first shaft and the second shaft are sequentially arranged along the first direction;

the first shaft is detachably connected with the first connecting structure along one end of the first direction close to the first connecting structure;

one end of the second shaft close to the second connecting structure is detachably connected with the second connecting structure; the second shaft is provided with a first channel extending along the first direction, and the first channel penetrates through one end, close to the first connecting structure, of the second shaft;

one end of the first shaft is arranged in the first channel, and the first shaft can move along the first channel.

In one embodiment, a sliding part is convexly arranged on the outer peripheral surface of the first shaft; the inner peripheral surface of the second shaft is provided with a second channel extending along the first direction, and the second channel is configured to guide the sliding piece to move along the second channel.

In one embodiment, four sliding pieces are arranged on the outer peripheral surface of the first shaft at intervals along the circumferential direction of the first shaft, and the included angle between any two adjacent sliding pieces is 90 degrees; and four second channels which correspond to the four sliding pieces one to one are arranged on the inner circumferential surface of the second shaft at intervals along the circumferential direction of the second shaft.

In one embodiment, the telescopic stirring shaft further comprises a first positioning piece; the inner circumferential surface of one end of the second shaft, which is close to the first connecting structure along the first direction, is provided with a first mounting groove, and the first positioning piece is mounted in the first mounting groove and used for limiting the movement of the first shaft along the first channel.

In one embodiment, the telescopic stirring shaft further comprises a second positioning piece; a second mounting groove is formed in the inner circumferential surface of one end, close to the first connecting structure, of the second shaft along the first direction, and the second positioning piece is mounted in the second mounting groove;

along first direction, the second setting element is located the upstream of first setting element, the second mounting groove is located the upstream of first mounting groove.

In one embodiment, the telescopic agitator shaft further comprises a first seal; the first sealing element is arranged between the first positioning element and the second positioning element and used for sealing a gap between the first shaft and the second shaft.

In one embodiment, the telescopic stirring shaft further includes a buffer member, and the buffer member is disposed on a side of the first shaft away from the first connecting structure.

In one embodiment, the bottom of the bioreactor is provided with a clamping groove; the second connecting structure includes:

the base is arranged at the bottom of the biological reaction bag, and the base part is positioned outside the biological reaction bag;

the pressing piece is movably arranged at one end, far away from the first connecting structure, of the base along the first direction;

the fastening piece extends into the pressing piece and is connected with the base, and the fastening piece is used for limiting the pressing piece;

one end of the first elastic piece is connected with the pressing piece, and the other end of the first elastic piece can be clamped in the clamping groove of the bioreactor; and

the second elastic piece is sleeved on the fastening piece and is positioned between the base and one end of the first elastic piece connected with the base; the second elastic piece is configured to drive the pressing piece to move in the first direction towards a direction away from the second elastic piece by means of the self deformation restoring force;

the pressing piece is configured to move in the first direction towards the direction close to the second elastic piece in response to the action of external force, so that the first elastic piece is separated from the clamping groove.

According to another aspect of the present application, there is provided a bioreactor further comprising a reactor housing having a second receiving chamber and the bioreactor bag described above, wherein the bioreactor bag is detachably mounted to the reactor housing and located in the second receiving chamber.

Drawings

FIG. 1 is a schematic view showing a telescopic stirring shaft of a bioreactor bag in a contracted state according to an embodiment of the present application;

FIG. 2 is a schematic view showing the structure of the telescopic stirring shaft of the bioreactor bag in an extended state according to an embodiment of the present application;

FIG. 3 illustrates an enlarged, partial schematic view of a first connection structure in an embodiment of the present application;

FIG. 4 is an enlarged, fragmentary schematic view of a second connection structure in an embodiment of the present application;

FIG. 5 illustrates a partial enlarged schematic view of a first shaft and a second shaft seal in accordance with an embodiment of the present application;

FIG. 6 shows a schematic view of the assembly of a bioreactor with a bioreactor bag according to an embodiment of the present application;

FIG. 7 is a schematic view showing the bioreactor of FIG. 6 according to the present application after the bioreactor bag is removed;

FIG. 8 is a partially enlarged view of the structure of the bioreactor section of FIG. 7 according to the present application.

The reference numbers illustrate:

100. a bioreactor; 10. a biological reaction bag; 11. a bag body; 111. a first accommodating chamber; 12. a first connecting structure; 121. a fourth connecting member; 122. a second connecting member; 1221. a first connection hole; 1222. a second connection hole; 123. a third connecting member; 1231. a third connection hole; 124. a second seal member; 13. a second connecting structure; 131. a base; 1311. an installation space; 1313. a first mounting hole; 1314. a sealing part; 132. a pressing member; 1321. a second mounting hole; 133. a fastener; 134. a first elastic member; 135. a second elastic member; 14. a telescopic stirring shaft; 141. a first shaft; 1411. a slider; 142. a second shaft; 1421. a first channel; 1422. a second channel; 1423. a first mounting groove; 1424. a second mounting groove; 1425. a sleeve; 143. a first positioning member; 144. a second positioning member; 145. a first seal member; 146. a buffer member; 15. a paddle; 16. a stirring structure; 161. a stirring member; 162. a mounting member; 1621. a fourth connection hole; 17. a flow divider; 171. a diverter body; 1711. an intake passage; 1712. a gas distribution cavity; 20. a reactor shell; 21. a second accommodating chamber; 30. a motor; 31. an output shaft; 40. a mounting seat; 50. a first connecting member; 51. a first connection portion; 52. a second connecting portion; 60. a fixing member; x: a first direction.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. As used herein, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are for purposes of illustration only and do not denote a single embodiment.

Fig. 1 shows a schematic structural diagram of a telescopic stirring shaft of a bioreactor bag in a contracted state in an embodiment of the present application, and fig. 2 shows a schematic structural diagram of a telescopic stirring shaft of a bioreactor bag in an extended state in an embodiment of the present application.

Referring to fig. 1 and 2 in combination with fig. 6 to 8, the present application provides a bioreactor bag 10 for a bioreactor 100, wherein the bioreactor bag 10 includes a bag body 11, a first connecting structure 12, a second connecting structure 13 and a telescopic stirring shaft 14. The first connecting structure 12 and the second connecting structure 13 are sequentially arranged along a first direction, the first connecting structure 12 and the second connecting structure 13 are respectively used for detachably connecting two opposite ends of the bioreactor 100 along the first direction (as shown by X in figure 1), the telescopic stirring shaft 14 is arranged between the first connecting structure 12 and the second connecting structure 13, and the size of the telescopic stirring shaft along the first direction is adjustable. Wherein, the bioreactor 100 comprises a motor 30 and a first connecting piece 50 connected with the motor 30, the first connecting structure 12 comprises a second connecting piece 122 detachably connected with the first connecting piece 50, the telescopic stirring shaft 14 and the second connecting piece 122 are coaxially arranged, and the first connecting piece 50 and the second connecting piece 122 are configured to enable the motor 30 and the telescopic stirring shaft 14 to be coaxially arranged.

Thus, the bioreactor bag 10 is arranged on the bioreactor 100 by arranging the first connecting structure 12 and the second connecting structure 13, and when the bioreactor bag 10 is replaced, because the first connecting structure 12 and the second connecting structure 13 are detachably connected with the bioreactor 100, the disassembly and assembly of the bioreactor bag 10 are simpler, and the time is saved; moreover, the telescopic stirring shaft 14 is in a contracted state before the biological reaction bag 10 is installed in the bioreactor 100, so that the transportation is convenient, after the old biological reaction bag 10 is detached, the new biological reaction bag 10 is connected with the bioreactor 100 through the second connecting structure 13, and then the telescopic stirring shaft 14 is lengthened to connect the first connecting structure 12 with the bioreactor 100, so that the assembly of the biological reaction bag 10 and the bioreactor 100 is completed, and the operation is simple; through setting up first connecting piece 50 and second connecting piece 122 to make motor 30 and the coaxial setting of flexible (mixing) shaft 14, avoid influencing the stirring effect of flexible (mixing) shaft 14.

The bag body 11 is rectangular as a whole and has a first accommodating chamber 111, and the first accommodating chamber 111 is used for accommodating other structures of the biological reaction bag 10 such as the telescopic stirring shaft 14 and gas and liquid required for performing a biological experiment.

Fig. 3 is a partially enlarged schematic view of a first connection structure in an embodiment of the present application.

One end of the first coupling structure 12 in the first direction is detachably coupled to one end of the bioreactor 100 in the first direction, and the other end is coupled to one end of the bioreactor bag 10 in the first direction. Specifically, the first connection structure 12 includes a fourth connection member 121, a second connection member 122, and a third connection member 123 that are coaxially disposed, the second connection member 122 connects one end of the fourth connection member 121 away from the second connection structure 13 along the first direction, and the second connection member 122 can rotate around its axis relative to the fourth connection member 121. The second connector 122 is provided with a plurality of first connection holes 1221 at intervals along a circumferential direction thereof, the first connection holes 1221 penetrate the second connector 122 along the first direction, and the second connector 122 is detachably connected to one end of the bioreactor 100 along the first direction by means of the first connection holes 1221. Second connecting hole 1222 has still been seted up along first direction to second connecting piece 122, and whole first connecting hole 1221 sets up around second connecting hole 1222. One end of the third connecting member 123 in the first direction is fixedly connected to the second connecting member 122 through the second connecting hole 1222, and the third connecting member 123 can rotate around its axis relative to the fourth connecting member 121. The other end of the third connecting member 123 along the first direction is provided with a third connecting hole, and the third connecting hole is used for detachably connecting the telescopic stirring shaft 14. In this way, the first connecting structure 12 is provided, and the second connecting member 122 is detachably connected to the bioreactor 100, so as to facilitate the disassembly and assembly of the bioreactor bag 10.

Optionally, the fourth connecting member 121 is connected with the second connecting member 122 through a bearing; the second connecting member 122 is a coupling; the second connecting hole 1222 is a threaded hole, and the outer circumferential surface of the portion of the third connecting member 123 connected to the second connecting member 122 is provided with threads, that is, the second connecting member 122 is connected to the third connecting member 123 through threads; a bearing is arranged between the third connecting member 123 and the fourth connecting member 121, so that the third connecting member 123 can rotate around the axis thereof relative to the fourth connecting member 121.

In some embodiments, the first connection structure 12 further includes a second seal 124. The second sealing member 124 is closely attached to one end of the third connecting member 123, and the second sealing member 124 is configured to be elastically deformed, and the second sealing member 124 seals the bioreactor bag 10 by the elastic deformation.

Fig. 4 is a partially enlarged schematic view of a second connection structure in an embodiment of the present application.

One end of the second connecting structure 13 along the first direction is detachably connected to one end of the bioreactor 100 away from the first connecting structure 12 along the first direction, and the other end is connected to one end of the bioreactor bag 10 away from the first connecting structure 12 along the first direction. Specifically, the second connecting structure 13 includes a base 131, a pressing member 132, a fastening member 133, a first elastic member 134, and a second elastic member 135. The base 131 is provided at the bottom of the bioreactor bag 10, and the base 131 is partially located at the outside of the bioreactor bag 10, and the base 131 is provided with an installation space 1311 and a first installation hole 1313 communicating with the installation space 1311. The pressing member 132 is movably disposed at one end of the base 131 away from the first connecting structure 12 along the first direction, and the pressing member 132 is disposed with a second mounting hole 1321 disposed coaxially with the first mounting hole 1313. The fastening member 133 protrudes into the pressing member 132 and is connected to the base 131, the fastening member 133 serves to limit the pressing member 132, and the fastening member 133 is configured to guide the movement of the pressing member 132 in the first direction. That is, the fastening member 133 extends into the second mounting hole 1321 and is coupled to the base 131 by the first mounting hole 1313, thereby limiting the moving range of the pressing member 132 in the first direction. Optionally, the fastener 133 is a stud.

In some embodiments, the base 131 further has a sealing portion 1314, the sealing portion 1314 is annular and is disposed around the base 131 along the outer circumference of the base 131, the sealing portion 1314 is located at a side of the installation space 1311 away from the stirring structure 16, and the sealing portion 1314 is located in the bioreactor bag 10 and is pressed by an external force or an adhesive to be connected to the bioreactor bag 10. It is understood that the seal 1314 may be attached to the bioreactor bag 10 in other ways. By providing the seal 1314 on the bottom plate 131 in this manner, the bottom of the bioreactor bag 10 can be sealed, and the liquid in the bioreactor bag 10 can be prevented from flowing out. It can be appreciated that when the fastening member 133 is a stud, an end of the stud away from the first connecting structure 12 in the first direction is a stud head, and an outer diameter of the stud head is larger than an aperture of the second mounting hole 1321, thereby limiting the pressing member 132; an outer diameter of an end of the stud, which is away from the stud head in the first direction, is smaller than a bore diameter of the second mounting hole 1321 to facilitate movement of the pressing piece 132 in the first direction.

The bioreactor 100 has a locking groove (not shown) formed at the bottom thereof, and the base 131 has a through hole (not shown) formed along a circumferential direction thereof to communicate with the installation space 1311. One end of the first elastic member 134 is connected to the pressing member 132, and the other end thereof extends into the through hole to be clamped in the clamping groove of the bioreactor 100, so that the bioreactor bag 10 is connected to the bioreactor 100. The second elastic member 135 is sleeved on the fastening member 133, and the second elastic member 135 is configured to drive the pressing member 132 to move along the first direction and away from the second elastic member 135 by a deformation restoring force of the second elastic member 135. The pressing member 132 is configured to move in a first direction toward the second elastic member 135 in response to an external force, so as to disengage the first elastic member 134 from the card slot.

Thus, by arranging the first elastic member 134, and clamping the first elastic member 134 in the clamping groove of the bioreactor 100, the second connecting structure 13 is connected to the bioreactor 100; by arranging the pressing member 132, the pressing member 132 can move in the first direction toward the direction close to the second elastic member 135 under the action of an external force to drive the first elastic member 134 to disengage from the clamping groove, so that the second connection structure 13 is separated from the bioreactor 100; by providing the second elastic member 135 and the pressing member 132 moving in the first direction toward the direction close to the second elastic member 135, the second elastic member 135 is in a compressed state, and after the external force is removed, the deformation restoring force of the second elastic member 135 drives the pressing member 132 to move in the first direction toward the direction away from the second elastic member 135 for restoration.

Referring to fig. 1 and 2, one end of the telescopic stirring shaft 14 along the first direction is detachably connected to the first connecting structure 12, the other end along the first direction is detachably connected to the second connecting structure 13, and the telescopic stirring shaft 14 is accommodated in the first accommodating cavity 111.

Fig. 5 is a partially enlarged schematic view of a portion of the first shaft and the second shaft at the seal in an embodiment of the present application.

Referring to fig. 1, 2 and 5, in particular, the telescopic stirring shaft 14 includes a first shaft 141 and a second shaft 142 connected to the first shaft 141, which are sequentially arranged in a first direction. The first shaft 141 is detachably connected to the first connecting structure 12 at an end close to the first connecting structure 12 along the first direction, and the second shaft 142 is detachably connected to the second connecting structure 13 at an end close to the second connecting structure 13. The second shaft 142 is provided with a first channel 1421 extending along a first direction, the first channel 1421 penetrates through an end of the second shaft 142 close to the first connecting structure 12, an end of the first shaft 141 is disposed in the first channel 1421, and the first shaft 141 can move along the first channel 1421.

So, through setting up flexible (mixing) shaft 14, when the transportation, can make primary shaft 141 shrink in primary channel 1421 of secondary shaft 142 to the transportation, when installation biological reaction bag 10, connect bioreactor 100 with second connection structure 13 earlier, the tensile first connection structure 12 of rethread, so that primary shaft 141 stretches out primary channel 1421, until first connection structure 12 can be connected with bioreactor 100, biological reaction bag 10's installation is simple, and can not destroy the bag body 11.

In some embodiments, the first shaft 141 has a sliding member 1411 protruding from an outer peripheral surface thereof, the second shaft 142 has an inner peripheral surface thereof formed with a second channel 1422 extending along the first direction, and the second channel 1422 is configured to guide the sliding member 1411 to move along the second channel 1422.

In some embodiments, four sliding members 1411 are disposed on the outer circumferential surface of the first shaft 141 at intervals in the circumferential direction of the first shaft 141, an included angle between any two adjacent sliding members 1411 is 90 degrees, and four second channels 1422 corresponding to the sliding members 1411 are disposed on the inner circumferential surface of the second shaft 142 at intervals in the circumferential direction of the second shaft 142. Optionally, the slide 1411 is a pin.

In this way, the second channel 1422 is provided to guide the slider 1411 to move along the second channel 1422, so as to prevent the first shaft 141 from tilting during extending or retracting of the first channel 1421, and the second channel 1422 and the slider 1411 are provided to facilitate the movement of the first shaft 141 along the first channel 1421, so that the movement of the first shaft 141 is controllable.

In some embodiments, the telescopic stirring shaft 14 further includes a first positioning element 143, an inner circumferential surface of the second shaft 142 near one end of the first connecting structure 12 along the first direction is provided with a first mounting groove 1423, and the first positioning element 143 is mounted in the first mounting groove 1423 for limiting movement of the first shaft 141 along the first passage 1421.

In some embodiments, the telescopic stirring shaft 14 further includes a second positioning member 144, an inner circumferential surface of the second shaft 142 near one end of the first connecting structure 12 along the first direction is provided with a second installation groove 1424, and the second positioning member 144 is installed in the second installation groove 1424. In the first direction, the second positioning member 144 is located upstream of the first positioning member 143, and the second mounting groove 1424 is located upstream of the first mounting groove 1423.

In this way, by providing the first positioning element 143 and the second positioning element 144, the movement of the first shaft 141 along the first channel 1421 can be limited, and the first shaft 141 is prevented from separating from the second shaft 142.

In some embodiments, the telescopic stirring shaft 14 further comprises a first sealing element 145, and the first sealing element 145 is disposed between the first positioning element 143 and the second positioning element 144 for sealing a gap between the first shaft 141 and the second shaft 142. In this manner, by providing first sealing member 145, the liquid in bag body 11 can be prevented from entering first passage 1421 through the gap between first shaft 141 and second shaft 142.

In some embodiments, the second shaft 142 further includes a sleeve 1425, the sleeve 1425 is sleeved on the second shaft 142, and the sleeve 1425 is detachably connected to the second shaft 142. It is understood that, in this embodiment, the first shaft 141 is movably disposed on the sleeve 1425 along the first direction, and the first channel 1421, the second channel 1422, the first mounting groove 1423 and the second mounting groove 1424 are correspondingly disposed on the inner circumferential surface of the sleeve 1425.

In some embodiments, the telescopic stirring shaft 14 further comprises a buffer 146, and the buffer 146 is disposed on a side of the first shaft 141 away from the first connecting structure 12. In this manner, by providing the buffer 146, the first shaft 141 can be buffered to reduce the impact of the first shaft 141 on the bottom wall of the first channel 1421 during contraction.

In some embodiments, the bioreactor bag 10 further includes a paddle 15, the paddle 15 is sleeved on the second shaft 142 of the telescopic stirring shaft 14, and the first shaft 141 can drive the second shaft 142 to rotate in response to an external force, so that the second shaft 142 drives the paddle 15 to rotate. It is understood that the paddle 15 is fixedly connected with the second shaft 142, and a plurality of paddles 15 can be provided, and all the paddles 15 are installed on the second shaft 142 at intervals along the axial direction of the second shaft 142. In this way, by providing a plurality of paddles 15, the mixing of the gas and the liquid in the bioreactor bag 10 is facilitated, and the flow of the liquid can be accelerated, thereby improving the uniformity of the mixing of the gas and the liquid.

In some embodiments, bioreactor bag 10 further includes a stirring structure 16 and a flow splitter 17. An installation space 1311 is provided at an end of the base 131 far from the pressing member 132 along the first direction, the stirring structure 16 can be partially accommodated in the installation space 1311, and the stirring structure 16 is configured to be rotatably inserted into the base 131 relative to the base 131 and located in the first accommodating cavity 111 of the bag body 11. Specifically, stirring structure 16 includes stirring bar 161, stirring bar 161 is a revolving body structure as a whole, stirring bar 161 is disposed on base 131 and located in installation space 1311, stirring bar 161 is configured to be rotatable with respect to base 131, and stirring bar 161 has a plurality of stirring portions (not shown in the figure) sequentially arranged along its circumferential direction.

In some embodiments, the stirring section has a curved surface with a starting edge and a terminating edge joining two stirring sections adjacent to the stirring section where the curved surface is located, the starting edge being located upstream or downstream of the terminating edge in the first direction. So, the curved surface extends along two not equidirectional simultaneously, can make the curved surface personally submit the heliciform, is favorable to stirring portion stirring liquid.

More specifically, in one embodiment, the stirring member 161 is provided with a plurality of receiving cavities (not shown) spaced apart along a circumferential direction thereof and extending through the stirring member 161 along the first direction, and each stirring portion is received in one of the receiving cavities. In another embodiment, a plurality of first grooves (not shown) are formed at intervals on one side of the stirring portion along the first direction, a plurality of second grooves corresponding to the first grooves one to one are formed at intervals on the other side of the stirring portion along the first direction, and each first groove, one corresponding second groove and the stirring member 161 define together to form one stirring portion. Alternatively, the stirring member 161 is injection molded using a mold.

In this way, by providing the stirring structure 16, and the stirring structure 16 includes the stirring member 161 having the stirring portion, the stirring member 161 can stir the liquid at the bottom and the side of the flow divider 17, so that the liquid at the bottom and the side of the flow divider 17 flows upward, and the fluidity of the liquid at the bottom and the side of the flow divider 17 is increased, so that the liquid at the bottom and the side of the flow divider 17 can be mixed with the gas.

In some embodiments, mixing structure 16 further includes mounting member 162, mounting member 162 being of a generally solid of revolution configuration, and mounting member 162 being coincident with the axis of mixing element 161. The mounting member 162 is connected to a side of the stirring member 161 close to the flow divider 17 in the first direction, and the whole stirring part is disposed around the mounting member 162. Wherein, the mounting member 162 is opened with a fourth connecting hole 1621 penetrating the mounting member 162 along the axial direction thereof, and the mounting member 162 is connected to the telescopic agitating shaft 14 through the fourth connecting hole 1621.

Thus, under the driving of the driving member, the whole stirring structure 16 can rotate relative to the base 131, so as to stir the liquid at the bottom and the side of the flow divider 17, so that the liquid at the bottom and the side of the flow divider 17 flows upwards, which is beneficial to improving the uniformity of the mixture of the liquid and the gas in the bag 11.

In some embodiments, the bioreactor bag 10 further comprises a bearing (not shown), and the stirring member 161 further defines a bearing mounting hole coaxially disposed therewith, wherein the diameter of the bearing mounting hole is smaller than the diameter of the inner wall of the accommodating cavity. The bearing is installed on the base 131 and located in the bearing installation hole, and is used for reducing friction between the stirring structure 16 and the base 131 in the rotating process.

The shunt 17 is connected to the base 131 and located in the first receiving cavity 111 of the bag body 11. Specifically, the splitter 17 is sleeved outside the mounting member 162 of the stirring structure 16, and both the stirring member 161 and the mounting member 162 of the stirring structure 16 are partially protruded outside the splitter 17. More specifically, the flow divider 17 includes a flow divider body 171 and a cover (not shown in the figure), the flow divider body 171 is connected to the base 131, the flow divider body 171 is provided with an air inlet passage 1711 and an air distribution cavity 1712 communicated with the air inlet passage 1711, and an axis of the air inlet passage 1711 extends in a direction perpendicular to an axis of the stirring structure 16. The gas distributing cavity 1712 is annular, the axis of the gas distributing cavity 1712 is coincident with the axis of the stirring structure 16, and gas in the gas inlet pipeline of the biological reaction bag 10 can sequentially pass through the gas inlet passage 1711 and the gas distributing cavity 1712.

The cover body is annular, is connected to one side of the shunt body 171 far away from the base 131 and covers the gas distributing cavity 1712, and is provided with a plurality of air holes (not shown in the figure) communicated with the gas distributing cavity 1712 at intervals along the circumferential direction, and all the air holes penetrate through the cover body along the axial direction of the cover body. The cover may be connected to the shunt body 171 by various methods such as pressing or welding, which is not limited herein. Optionally, the pore size of the pores ranges from 0.3mm to 1.2mm; preferably, the pore diameter of the pores is 0.8mm.

Thus, by providing the air inlet passage 1711, the air distribution chamber 1712, and the air holes which are sequentially communicated, the air can flow into the bag body 11 through the air inlet passage 1711, the air distribution chamber 1712, and the air holes in sequence, so that the air is mixed with the liquid in the bag body 11.

In some embodiments, the base 131 has a locking portion (not shown), and the shunt body 171 has a locking groove (not shown), and the locking portion can be mounted on the shunt body 171 via the locking groove. Optionally, the base 131 is provided with a plurality of clamping portions at intervals along the circumferential direction of the installation cavity, the shunt member body 171 is also provided with a plurality of clamping grooves at intervals along the circumferential direction of the stirring structure 16, and each clamping portion can be clamped in one corresponding clamping groove.

In some embodiments, the flow splitter body 171 is opened with a plurality of through hole flow holes, and all the through hole flow holes penetrate through the flow splitter body 171 along the first direction, so as to allow the liquid in the bag 11 at two opposite sides of the flow splitter 17 to flow. All the through-hole communication holes are provided in the flow distribution member body 171 at intervals in the circumferential direction of the flow distribution member body 171, and the air distribution chamber 1712 is provided around all the through-hole communication holes. It is understood that the number and diameter of the through-hole flow holes may be set as desired, and the through-hole flow holes may be set one turn or more turns around the axis of the gas-distributing chamber 1712.

FIG. 6 shows a schematic view of the assembly of a bioreactor with a bioreactor bag according to an embodiment of the present application; FIG. 7 is a schematic view showing the bioreactor of FIG. 6 according to the present application after the bioreactor bag is removed; FIG. 8 is a partially enlarged view of the structure of the bioreactor section of FIG. 7 according to the present application.

Referring to fig. 6 to 8, based on one general inventive concept, the present application further provides a bioreactor 100, the bioreactor 100 further includes a reactor housing 20 having a second receiving chamber 21, and a bioreactor bag 10 of any one of the above embodiments, the bioreactor bag 10 being detachably mounted to the reactor housing 20 and located in the second receiving chamber 21.

Specifically, the bioreactor 100 further includes a mount 40 connected to the motor 30 and a fixing member 60. The mounting seat 40 is a whole body of a rotating body, the mounting seat 40 is connected with the reactor shell 20, and the mounting seat 40 is used for fixedly connecting the motor 30. The first connecting member 50 is a rotating body, and one end of the first connecting member 50 along the first direction is connected to the output shaft 31 of the motor 30, and the other end is connected to the second connecting member 122. The first connecting member 50 includes a first connecting portion 51 and a second connecting portion 52 connected to the first connecting portion 51, and one end of the first connecting portion 51 away from the second connecting structure 13 along the first direction is connected to the output shaft 31 of the motor 30. More specifically, the first connecting portion 51 includes a first section (not shown in the figure) and a second section (not shown in the figure) connected to the first section, the first section is provided with a third mounting hole (not shown in the figure) along the first direction, and the output shaft 31 of the motor 30 is mounted in the third mounting hole; the second section has a plurality of fourth mounting holes (not shown in the figure) along the circumferencial direction interval of third holding chamber, and all fourth mounting holes run through the second section along first direction. The second connecting portion 52 includes a third section (not shown in the figure) and a plurality of fourth sections (not shown in the figure) that are disposed in one-to-one correspondence with the fourth mounting holes, all the fourth sections are connected to one side of the third section, which is close to the second connecting structure 13 along the first direction, at intervals along the circumferential direction of the third accommodating cavity, the third section is provided with a fifth mounting hole that penetrates through the third section along the first direction, and the first section penetrates through the fifth mounting hole (not shown in the figure). The fourth section and the first connection hole 1221 are arranged in a one-to-one correspondence manner, and one end of the fourth section, which is far away from the third section, can extend out of the fourth mounting hole, so that the fourth section is connected with the second connection member 122. Optionally, the fourth section is a pin, and an outer diameter of the fourth section is smaller than an aperture of the fourth mounting hole.

In this way, the output shaft 31 of the motor 30 is connected to the first connecting portion 51, and drives the first connecting portion 51 to drive the second connecting portion 52 to rotate, so as to drive the second connecting portion 122 connected to the first connecting portion 50 to rotate, thereby driving the telescopic stirring shaft 14 to rotate.

The fixing member 60 is sleeved on the outer peripheral surfaces of the second segment and the fourth connecting member 121 to fix the first connecting structure 12 and the first connecting member 50. Optionally, the fixture 60 is a clip.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent should be subject to the appended claims.

Claims

1. A bioreactor bag for a bioreactor, the bioreactor bag comprising:

a bag body having a first receiving cavity;

wherein, bioreactor includes the motor and connects the first connecting piece of motor, first connection structure include with first connecting piece can dismantle the second connecting piece that connects, flexible (mixing) shaft with the coaxial setting of second connecting piece, first connecting piece with the second connecting piece is configured to make the motor with flexible (mixing) shaft is coaxial to be set up.

2. The bioreactor bag according to claim 1, wherein the telescopic stirring shaft comprises a first shaft and a second shaft connected to the first shaft, which are sequentially arranged along the first direction;

3. The bioreactor bag according to claim 2, wherein a sliding member is protruded from an outer circumferential surface of the first shaft; the inner peripheral surface of the second shaft is provided with a second channel extending along the first direction, and the second channel is configured to guide the sliding piece to move along the second channel.

4. The bioreactor bag according to claim 3, wherein four sliding members are provided on the outer circumferential surface of the first shaft at intervals along the circumferential direction of the first shaft, and the included angle between any two adjacent sliding members is 90 degrees; and four second channels which correspond to the four sliding pieces one to one are arranged on the inner circumferential surface of the second shaft at intervals along the circumferential direction of the second shaft.

5. The bioreactor bag of claim 2, wherein said telescopic agitator shaft further comprises a first locator; the inner circumferential surface of one end of the second shaft, which is close to the first connecting structure along the first direction, is provided with a first mounting groove, and the first positioning piece is mounted in the first mounting groove and used for limiting the movement of the first shaft along the first channel.

6. The bioreactor bag of claim 5, wherein said telescopic stirring shaft further comprises a second positioning member; a second mounting groove is formed in the inner circumferential surface of one end, close to the first connecting structure, of the second shaft along the first direction, and the second positioning piece is mounted in the second mounting groove;

7. The bioreactor bag of claim 6, wherein said telescopic agitator shaft further comprises a first seal; the first sealing element is arranged between the first positioning element and the second positioning element and used for sealing a gap between the first shaft and the second shaft.

8. The bioreactor bag according to claim 2, wherein said flexible mixing shaft further comprises a buffer member disposed on a side of said first shaft remote from said first connecting structure.

9. The bioreactor bag according to any one of claims 1 to 8, wherein a neck is provided at the bottom of the bioreactor; the second connecting structure includes:

the second elastic piece is sleeved on the fastening piece and is positioned between the base and one end of the first elastic piece connected with the base; the second elastic piece is configured to drive the pressing piece to move along the first direction towards a direction away from the second elastic piece by means of self deformation restoring force;

10. A bioreactor comprising a reactor housing having a second receiving chamber and a bioreactor bag as claimed in any one of claims 1 to 9 removably mounted to the reactor housing and located within the second receiving chamber.