CN115785196A - Method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking - Google Patents
Method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking Download PDFInfo
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- 241001480978 Pyropia haitanensis Species 0.000 title claims abstract description 90
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 51
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000000605 extraction Methods 0.000 claims abstract description 25
- 238000001035 drying Methods 0.000 claims abstract description 18
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 16
- 239000002244 precipitate Substances 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 12
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000012870 ammonium sulfate precipitation Methods 0.000 claims description 7
- 239000012460 protein solution Substances 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 claims description 6
- 230000002000 scavenging effect Effects 0.000 claims description 6
- 238000011033 desalting Methods 0.000 claims description 5
- 238000000502 dialysis Methods 0.000 claims description 5
- 239000012154 double-distilled water Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 claims description 3
- 230000003078 antioxidant effect Effects 0.000 claims description 3
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 claims description 3
- 230000007760 free radical scavenging Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 4
- 238000004090 dissolution Methods 0.000 abstract description 2
- 238000005185 salting out Methods 0.000 abstract description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 230000035699 permeability Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 5
- 238000001704 evaporation Methods 0.000 description 5
- 238000000751 protein extraction Methods 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000001291 vacuum drying Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The invention discloses a method for efficiently extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking. The method comprises the following steps: s1: pretreating porphyra haitanensis; s2: processing porphyra haitanensis by ultrasonic-assisted wall breaking; s3: purifying crude porphyra haitanensis protein; s4: drying the crude protein of the porphyra haitanensis to obtain a finished product. The method has the advantages that the porphyra haitanensis protein is extracted by an ultrasonic-assisted aqueous solution extraction method, the permeability of cell membranes is changed by ultrasonic waves, the molecular movement rate is accelerated, the mutual collision of molecules is promoted, the dissolution of the protein is accelerated, and the product extraction rate is 50-58%; the protein is precipitated by a sulfuric acid salting-out method, the dissociation of the protein is inhibited, and the recovery rate of the protein is 72-85%.
Description
Technical Field
The invention belongs to the technical field of marine algae protein extraction, and particularly relates to a method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking.
Background
In recent years, with the continuous progress of cultivation technology and the gradual expansion of cultivation scale, people are attracted by the processing and utilization of porphyra haitanensis. At present, researches on porphyra haitanensis-derived active substances focus on extraction and activity researches of terpenoids and polysaccharides, and researches on porphyra haitanensis proteins and polypeptides are few. Therefore, the development of an efficient porphyra haitanensis protein extraction method has an important promotion effect on the development and utilization of porphyra haitanensis.
Since Porphyra haitanensis has tough cell wall, the extraction method can not achieve good extraction effect. In the extraction process of the porphyra haitanensis protein, the rigid cell wall is efficiently broken, which becomes the key of the extraction technology. In addition, the protein extract contains a large amount of polysaccharides, and the conventional centrifugal precipitation and isoelectric precipitation cannot realize good separation of protein and impurities.
Disclosure of Invention
The invention aims to provide a method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking, which has the characteristics of time saving and high efficiency.
In order to achieve the purpose, the method for extracting the porphyra haitanensis protein by ultrasonic-assisted wall breaking comprises the following specific steps:
s1: the pre-treatment of the porphyra haitanensis,
drying porphyra haitanensis in a constant-temperature drying oven at the temperature of 55-70 ℃ until the quality is constant;
pulverizing dried Porphyra haitanensis with high speed pulverizer, sieving with 200 mesh sieve, and sealing and storing at-20 deg.C (storage time, whether storage time is related to extraction rate or extraction efficiency);
s2: the porphyra haitanensis is treated by ultrasonic-assisted wall breaking,
preparing porphyra haitanensis powder into a solution (whether a solvent is water or not), adjusting the pH value to 6.0-8.0, and performing auxiliary treatment by adopting ultrasonic waves, wherein the ultrasonic frequency is 10-15 KHz, the mode is intermittent ultrasonic, the ultrasonic generation time and the ultrasonic interval time are both 6-8 s, the ultrasonic power is in the range of 800-1500W, and the ultrasonic whole-course time is 40-80 min; centrifuging for the first time after the ultrasonic treatment is finished, and taking supernatant;
s3: purifying the crude protein of the porphyra haitanensis,
under the ice bath condition, slowly adding ammonium sulfate powder into the supernatant while stirring until the saturation reaches 50-70%, standing for 2h, centrifuging the solution after ammonium sulfate precipitation again, and taking the precipitate to obtain crude protein;
re-dissolving the precipitate in double distilled water, regulating the pH value of the solution to be neutral, and dialyzing in a 200 Da dialysis bag for 24-48 h for desalting;
s4: the crude protein of the porphyra haitanensis is dried,
carrying out vacuum rotary evaporation and concentration on the dialyzed porphyra haitanensis protein solution to collect concentrated solution;
and (3) freezing the concentrated solution in an environment of-40 to-50 ℃, and drying in a vacuum freeze dryer for 12 to 24 hours to obtain porphyra haitanensis crude protein dry powder.
Preferably, the step S2 is performed with a first centrifugation after the ultrasonic treatment, wherein the first centrifugation condition is 10000 r.min -1 、4℃、20 min。
Preferably, in the step S3, the solution after ammonium sulfate precipitation is centrifuged again under the condition of 15000 r.min -1 、4℃、15 min。
Preferentially, the porphyra haitanensis protein solution dialyzed in the step S4 is subjected to vacuum rotary evaporation concentration under the concentration condition of 45-55 ℃ until the concentration is 200-300 mg -1 。
The crude porphyra haitanensis protein has stronger antioxidant activity within the range of 5-50 mg/mL, ABTS free radical scavenging capacity is equivalent to 0.83 +/-0.08 mmol/L Trolox, and DPPH scavenging rate reaches 68.04 +/-0.73% when the DPPH scavenging rate is 10 mg/mL.
The method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking has the beneficial effects that: according to the invention, through the processing processes of crushing, dissolving, ultrasonic-assisted water extraction and the like of the porphyra haitanensis, the cell wall of the porphyra haitanensis can be crushed, the dissolution of protein is promoted, the protein in the porphyra haitanensis is fully extracted, the extraction rate of the porphyra haitanensis protein is improved, the extraction time is effectively shortened, and the extraction cost is saved; the high-saturation ammonium sulfate can precipitate protein, so that the recovery rate is high; in the process of precipitating protein by an ammonium sulfate salting-out method, along with the increase of the saturation of ammonium sulfate, the recovery rate of the protein in the precipitation is increased firstly and then decreased, and the maximum value is reached when the saturation of the ammonium sulfate is 50-70 percent; according to the method, the protein content of the product is detected by a BCA kit method, and the recovery rate of the protein in the precipitate is 72-85 percent.
Drawings
FIG. 1 shows the effect of pH on the extraction rate of Porphyra haitanensis protein;
FIG. 2 shows the protein properties of Porphyra haitanensis.
Detailed Description
Example 1
The invention relates to a method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking, which comprises the following specific steps:
s1: pretreating porphyra haitanensis, namely drying the porphyra haitanensis in a constant-temperature drying oven at the temperature of 55 ℃ until the quality is constant; pulverizing dried Porphyra haitanensis with high speed pulverizer, sieving with 200 mesh sieve, and storing at-20 deg.C under sealed condition;
s2: ultrasonic-assisted wall breaking treatment of porphyra haitanensis, preparing porphyra haitanensis powder into a solution, adjusting the pH value to 6.0, and performing auxiliary wall breaking treatment by adopting ultrasonic waves, wherein the ultrasonic frequency is 10 KHz, the mode is intermittent ultrasonic, the ultrasonic generation time and the ultrasonic interval time are both 6 s, the ultrasonic power is 1200W, and the whole ultrasonic time is 60 min; performing primary centrifugation after the ultrasonic treatment is finished, wherein the primary centrifugation condition is 10000 r.min -1 Centrifuging at 4 deg.C for 20 min, collecting supernatant, and determining protein extraction rate to be 50.11%;
s3: purifying crude protein of Porphyra haitanensis, adding ammonium sulfate powder slowly into supernatant under stirring in ice bath until saturation reaches 60%, standing for 2 hr, centrifuging again the solution after ammonium sulfate precipitation under 15000 r.min -1 Centrifuging at 4 deg.C for 15 min to obtain precipitate, measuring protein content in the residual supernatant, and calculating precipitate protein recovery rate to 72.13%; re-dissolving the precipitate in double distilled water, adjusting pH of the solution to 7.0, and dialyzing in 200 Da dialysis bag for 48 h for desalting;
s4: drying crude protein of Porphyra haitanensis, vacuum rotary evaporating and concentrating dialyzed Porphyra haitanensis protein solution to collect concentrated solution, vacuum rotary evaporating and concentrating at 45 deg.C, and concentrating to 200 mg -1 And (4) carrying out freeze vacuum drying at the cold trap temperature of-45 ℃ to obtain the porphyra haitanensis protein dry powder.
Example 2
The invention relates to a method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking, which comprises the following specific steps:
s1: pretreating porphyra haitanensis, namely drying the porphyra haitanensis in a constant-temperature drying oven at 60 ℃ until the quality is constant; pulverizing dried Porphyra haitanensis with a high-speed pulverizer, sieving with 200 mesh sieve, and storing at-20 deg.C under sealed condition;
s2: the porphyra haitanensis is treated by ultrasonic-assisted wall breaking,preparing porphyra haitanensis powder into a solution, adjusting the pH value to 7.0, and performing auxiliary wall breaking treatment by adopting ultrasonic waves, wherein the ultrasonic frequency is 15 KHz, the mode is intermittent ultrasonic, the ultrasonic generation time and the ultrasonic interval time are both 7 s, the ultrasonic power is 1000W, and the whole ultrasonic process time is 60 min; performing primary centrifugation after the ultrasonic treatment is finished, wherein the primary centrifugation condition is 10000 r.min -1 Centrifuging at 4 deg.C for 20 min, collecting supernatant, and determining protein extraction rate to be 58.12%;
s3: purifying crude protein of Porphyra haitanensis, adding ammonium sulfate powder slowly into supernatant under stirring in ice bath until saturation reaches 65%, standing for 2 hr, centrifuging again the solution after ammonium sulfate precipitation under 15000 r.min -1 Centrifuging at 4 deg.C for 15 min to obtain precipitate, measuring protein content in the remaining supernatant, and calculating precipitate protein recovery rate to 85.16%; re-dissolving the precipitate in double distilled water, adjusting pH of the solution to 7.0, and dialyzing in 200 Da dialysis bag for 48 h for desalting;
s4: drying crude protein of Porphyra haitanensis, vacuum rotary evaporating and concentrating dialyzed Porphyra haitanensis protein solution to collect concentrated solution, vacuum rotary evaporating and concentrating at 45 deg.C, and concentrating to 200 mg -1 And (4) carrying out freeze vacuum drying at the cold trap temperature of-45 ℃ to obtain the porphyra haitanensis protein dry powder.
Example 3
The invention relates to a method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking, which comprises the following specific steps:
s1: pretreating porphyra haitanensis, namely drying the porphyra haitanensis in a constant-temperature drying oven at 70 ℃ until the quality is constant; pulverizing dried Porphyra haitanensis with high speed pulverizer, sieving with 200 mesh sieve, and storing at-20 deg.C under sealed condition;
s2: processing porphyra haitanensis by ultrasonic wave assisted wall breaking, preparing porphyra haitanensis powder into a solution, adjusting the pH value to 7.0, and performing assisted wall breaking by ultrasonic waves, wherein the ultrasonic frequency is 15 KHz, the mode is intermittent ultrasonic, the ultrasonic generation time and the ultrasonic interval time are both 8 s, the ultrasonic power is 1500W, and the ultrasonic whole-process time is 70 min; performing primary centrifugation after the ultrasonic treatment, wherein the primary centrifugation condition is 10000 r.min -1 4 ℃ CCentrifuging for 20 min, collecting supernatant, and determining protein extraction rate to be 54.32%;
s3: purifying crude protein of Porphyra haitanensis, slowly adding ammonium sulfate powder into supernatant under stirring in ice bath until saturation reaches 70%, standing for 2 hr, centrifuging the solution after ammonium sulfate precipitation again at 15000 r.min -1 Centrifuging at 4 deg.C for 15 min to obtain precipitate, measuring protein content in the residual supernatant, and calculating precipitate protein recovery rate to 81.44%; re-dissolving the precipitate in double distilled water, adjusting pH of the solution to 7.0, and dialyzing in 200 Da dialysis bag for 48 h for desalting;
s4: drying crude Porphyra haitanensis protein, vacuum rotary evaporating dialyzed Porphyra haitanensis protein solution at 50 deg.C, concentrating to 300 mg.ml -1 And (4) carrying out freeze vacuum drying at the cold trap temperature of-45 ℃ to obtain the porphyra haitanensis protein dry powder.
Single factor test: supplement
1. Porphyra haitanensis can be added55℃～70℃Drying in a constant-temperature drying oven until the quality is constant, wherein the temperature influences the extraction rate;
2. influence of the high-speed pulverizer on the extraction rate;-20℃influence of the sealing preservation temperature on the extraction rate;
3.pH 6.0～8.0influence on extraction yield;
within the range of pH 3-9, the influence of the pH value of the solution on the extraction rate of the porphyra haitanensis protein is obvious (P < 0.05). As the pH increases, the extraction rate shows a tendency to increase first and then to equilibrate, reaching a maximum when the pH is 7.
The emulsifying activity of the porphyra haitanensis protein can reach 112.49 +/-5.33 m at the pH value of 5.5 2 The emulsion stability is 40.52 +/-1.08 min, and the protein has the potential of being developed into a natural emulsifier and can be subjected to protein modification through physical, chemical and biological methods to obtain better emulsion property.
The foam stability of the porphyra haitanensis protein reaches 80.84 +/-2.95% at the pH value of 4.5, which indicates that the protein extracted by the method has good foam stability.
4. Performing auxiliary wall breaking treatment by ultrasonic waves, wherein ultrasonic parameters influence the extraction rate;
5. influence of the first centrifugation condition on the extraction rate;
influence of re-centrifugation conditions on extraction yield;
6. the effect of ammonium sulfate powder on extraction yield;
7. and (4) measuring the protein property of the porphyra haitanensis.
The porphyra haitanensis protein has stronger antioxidant activity within the range of 5 to 50 mg/mL, the ABTS free radical scavenging capacity is equivalent to 0.83 +/-0.08 mmol/L Trolox, and the DPPH scavenging rate reaches 68.04 +/-0.73 percent when the DPPH scavenging rate is 10 mg/mL.
The isoelectric point of the porphyra haitanensis protein is 4.5.
As is clear from FIG. 2, the protein water absorption of Porphyra haitanensis was 1.797ml/g, the oil absorption was 0.998ml/g, and the water retention was 1.640g/g.
Claims (5)
1. A method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking is characterized by comprising the following steps: the method comprises the following specific steps:
s1: the pre-treatment of the porphyra haitanensis,
drying porphyra haitanensis in a constant-temperature drying oven at the temperature of 55-70 ℃ until the quality is constant;
pulverizing dried Porphyra haitanensis with high speed pulverizer, sieving with 200 mesh sieve, and sealing and storing at-20 deg.C (whether the storage time of thermometer is related to extraction rate or extraction efficiency);
s2: the porphyra haitanensis is treated by ultrasonic-assisted wall breaking,
preparing porphyra haitanensis powder into a solution (whether a solvent is water or not), adjusting the pH value to 6.0-8.0, and performing auxiliary treatment by adopting ultrasonic waves, wherein the ultrasonic frequency is 10-15 KHz, the mode is intermittent ultrasonic, the ultrasonic generation time and the ultrasonic interval time are both 6-8 s, the ultrasonic power is in the range of 800-1500W, and the ultrasonic whole-course time is 40-80 min; centrifuging for the first time after the ultrasonic treatment is finished, and taking supernate;
s3: purifying the crude protein of the porphyra haitanensis,
under the ice bath condition, slowly adding ammonium sulfate powder into the supernatant while stirring until the saturation reaches 50-70%, standing for 2h, centrifuging the solution after ammonium sulfate precipitation again, and taking the precipitate to obtain crude protein;
re-dissolving the precipitate in double distilled water, regulating the pH value of the solution to be neutral, and dialyzing in a 200 Da dialysis bag for 24-48 h for desalting;
s4: the crude protein of the porphyra haitanensis is dried,
carrying out vacuum rotary evaporation and concentration on the dialyzed porphyra haitanensis protein solution to collect concentrated solution;
and (3) freezing the concentrated solution in an environment of-40 to-50 ℃, and drying in a vacuum freeze dryer for 12 to 24 hours to obtain porphyra haitanensis crude protein dry powder.
2. The method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking as claimed in claim 1, wherein the method comprises the following steps: in the step S2, the first centrifugation is carried out after the ultrasonic treatment, and the first centrifugation condition is 10000 r.min -1 、4℃、20 min。
3. The method for extracting porphyra haitanensis protein through ultrasonic-assisted wall breaking according to claim 1, wherein the method comprises the following steps: in the step S3, the solution after the ammonium sulfate precipitation is centrifuged again, and the centrifugation condition is 15000 r.min -1 、4℃、15 min。
4. The method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking as claimed in claim 1, wherein the method comprises the following steps: and (5) performing vacuum rotary evaporation and concentration on the porphyra haitanensis protein solution dialyzed in the step (S4) under the concentration condition of 45-55 ℃ to 200-300 mg/ml -1 。
5. The method for extracting porphyra haitanensis protein by ultrasonic-assisted wall breaking as claimed in claim 1, wherein the method comprises the following steps: the porphyra haitanensis crude protein has stronger antioxidant activity within the range of 5 to 50 mg/mL, ABTS free radical scavenging capacity is equivalent to 0.83 +/-0.08 mmol/L Trolox, and DPPH scavenging rate reaches 68.04 +/-0.73% when the DPPH scavenging rate is 10 mg/mL.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865973A (en) * | 2014-04-03 | 2014-06-18 | 江南大学 | Preparation method of porphyra protein antibacterial peptide |
| CN107312076A (en) * | 2017-08-01 | 2017-11-03 | 中国科学院海洋研究所 | A kind of method that phycoerythrin is extracted in the dry product from Porphyra yezoensis |
| CN109734772A (en) * | 2019-03-11 | 2019-05-10 | 中国水产科学研究院南海水产研究所 | A kind of extracting method of end water porphyra haitanensis protein |
| US20210000140A1 (en) * | 2018-03-06 | 2021-01-07 | Ciimar - Centro Interdisciplinar De Investigaçäo Marinha E Ambiental | Method for obtaining proteins or a rich-protein extract from algae, extracts and uses therefore |
-
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- 2022-12-21 CN CN202211647476.5A patent/CN115785196A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865973A (en) * | 2014-04-03 | 2014-06-18 | 江南大学 | Preparation method of porphyra protein antibacterial peptide |
| CN107312076A (en) * | 2017-08-01 | 2017-11-03 | 中国科学院海洋研究所 | A kind of method that phycoerythrin is extracted in the dry product from Porphyra yezoensis |
| US20210000140A1 (en) * | 2018-03-06 | 2021-01-07 | Ciimar - Centro Interdisciplinar De Investigaçäo Marinha E Ambiental | Method for obtaining proteins or a rich-protein extract from algae, extracts and uses therefore |
| CN109734772A (en) * | 2019-03-11 | 2019-05-10 | 中国水产科学研究院南海水产研究所 | A kind of extracting method of end water porphyra haitanensis protein |
Non-Patent Citations (2)
| Title |
|---|
| LIN WEN等: "Ultrasound-assisted extraction and in vitro simulated digestion of Porphyra haitanensis proteins exhibiting antioxidative and α-glucosidase inhibitory activity", 《JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION》, vol. 14, 27 July 2020 (2020-07-27), pages 3291 * |
| 徐海菊等: "坛紫菜蛋白质提取工艺优化及其特性分析", 《食品工业科技》, vol. 43, no. 12, 7 January 2022 (2022-01-07), pages 1 * |
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