CN115768851A - 具有在恶劣环境中抑制硫化物产生的功效的代谢抑制剂 - Google Patents
具有在恶劣环境中抑制硫化物产生的功效的代谢抑制剂 Download PDFInfo
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Abstract
本申请公开了一种提供基于N‑羟基甲酰胺化合物的代谢抑制剂组合物的方法,已证明该代谢抑制剂组合物在厌氧条件下抑制硫化物产生的功效。该组合物适合用于井下、钻井和勘探应用环境和包括采矿、金属工业提取和污水处理的其他恶劣环境应用,以及非恶劣环境应用。
Description
技术领域
本发明涉及在有或没有杀生物剂的情况下通过使恶劣环境与基于N-羟基甲酰胺的代谢抑制剂组合物接触来抑制硫化物产生的方法。
背景技术
硫化物、特别是硫化氢(H2S)的生成是通过将含硫酸盐或其他含硫的水溶液引入内源微生物和含水、含油、含烃体系或任何其他可产生硫化氢的体系中含有的微生物的厌氧环境中而开始的。
硫化氢具有毒性、腐蚀性和易燃性,并且经常同时在上游和下游油气工业中引起问题。即使以低浓度暴露,也可能引起严重伤害或死亡。每年花费相当大的费用和精力来降低油气流的H2S含量,以使其适合于商业用途。因此,需要一种有效的方法来抑制硫化氢的生成并减少负责产生硫化氢的微生物的生长或将其杀死。
众所周知,异羟肟酸在文献中可用作具有强力抗疟活性的组蛋白脱乙酰酶抑制剂药物。还已经在文献中报道,这些异羟肟酸因其在治疗各种肿瘤和癌症中的治疗潜力而用于药物中,例如,如在Comprehensive Medicinal Chemistry II[综合药物化学II],2007中H.Weinmann、E.Ottow的章节“Therapeutic Areas II:Cancer,Infectious Diseases,Inflammation&Immunology and Dermatology[治疗领域II:癌症、传染病、炎症和免疫学及皮肤病学]”中描述的。
Yi-Yi Zhang,Cheng-He Zhou的文章“Synthesis and activities ofnaphthalimide azoles as a new type of antibacterial and antifungal agents[作为新型抗细菌剂和抗真菌剂的萘二甲酰亚胺唑(naphthalimide azole)的合成和活性]”,已经描述了萘二甲酰亚胺衍生物作为抗微生物剂的用途。该文章描述了通过使用这些衍生物杀死微生物的方法并且将这些作用归因于萘二甲酰亚胺骨架。这些化合物还可以增强药物特性,这指示骨架可以具有杀生物作用并且添加可以提高功效。
Mohammed R.Ahmad、Suaad M.H.Al-Majidi和Ayad Kareem Khan的文章“Synthesis,Evaluation Antimicrobial Activity of Some New N-substitutedNaphthalimides Containing Different Heterocyclic Rings[一些含有不同杂环的新型N取代的萘二甲酰亚胺的合成、抗微生物活性评估]”披露了一些新合成的与四元或五元杂环连接的萘二甲酰亚胺对四种类型的病原细菌和一种类型的真菌的抗细菌活性。发现这些化合物具有中至高的抗微生物活性。
Julija Matijevi-Sosa和Zdenka Cvetnic的文章“Antimicrobial activity ofN-phthaloylamino acid hydroxamates[N-邻苯二甲酰氨基酸异羟肟酸盐的抗微生物活性]”描述了N-邻苯二甲酰氨基酸异羟肟酸盐的抗细菌和抗真菌活性。发现异羟肟酸盐通过螯合革兰氏阳性细菌和革兰氏阴性细菌两者中的PDF酶金属以及革兰氏阴性酶中的LpxC酶来抑制生长。邻苯二甲酰亚胺似乎通过使m-RNA不稳定而有助于抑制,而抗真菌活性并没有很好地表达。
在US 5279967 A中,已披露了萘二甲酰亚胺衍生物N,N'-二烷基-4-氨基-1,8-萘二甲酰亚胺在油气工业中的用途。这些化合物已用于使用荧光标记化合物识别和追踪烃类。
US 6358746 B1披露了萘二甲酰亚胺衍生物在工业水溶液中的用途,用于在水系统如在石油行业中用作荧光示踪剂。
这两项专利都将衍生物用于油气应用,但它们并未在恶劣环境中、特别是在厌氧条件下使用以抑制硫化物产生。
已经证实,基于异羟肟酸盐的化合物具有抗微生物活性;然而,缺点是大多数异羟肟酸盐不具有在恶劣环境中发挥作用的稳定性和功效。要解决的问题是提供一种提供组合物的方法,该组合物可以在厌氧条件下抑制产生硫化物的生物体产生硫化物。
发明内容
本发明涉及一种抑制硫化物产生的方法,该方法包括:
(i)提供包含至少一种具有结构1的化合物的组合物:
其中,Z:C(O)NHOH或C(Y)(R),
Y:氢,C6芳香族基团,C6杂芳香族基团,C6脂肪族环状基团或脂环族基团,杂基团如硝基、磷酸根、羟基,
R:以N-羟基甲酰胺、羧酸、醇或N-羟基甲酰胺封端的碳(n=1-10)直链或支链化合物,
X:氢、OH、NH2、卤素、碳(n=1-3)直链或支链化合物;以及
(ii)使该组合物与产生硫化物的细菌在厌氧条件下接触,以抑制硫化物产生。
本发明还涉及一种抑制硫化物产生的方法,该方法包括:
(i)提供包含至少一种具有结构2的化合物的组合物:
其中,W是:氢,任选地以羟基酰胺、羧酸、醇或N-羟基甲酰胺封端的碳(n=1-10)直链或支链化合物,C6芳香族基团,C6杂芳香族基团,C6脂肪族环状基团或脂环族基团;以及
(ii)使该组合物与产生硫化物的细菌在厌氧条件下接触,以抑制硫化物产生。
具体实施方式
这些组合物已证明具有抑制硫化物产生的功效。这些组合物适合用于包括井下、钻井和勘探应用以及油气环境的硫化物存在的水性环境以及包括采矿、金属工业提取和污水和废水处理的其他恶劣环境应用,以及非恶劣环境应用。
在描述本发明时,使用了许多术语。除非另外说明,否则如下定义这些术语:
如本文所用,在本发明的要素或组分前的冠词“一个/种(a/an)”和“该/所述(the)”在关于该要素或组分的实例(即,出现)的数目旨在是非限制性的。因此,“一个/种(a/an)”和“该/所述(the)”应理解为包括一个/种或至少一个/种,并且要素或组分的单数词语形式还包括复数,除非该数字明显意指单数。
如本文所用,术语“包含”意指如权利要求中所提及的说明的特征、整数、步骤或组分的存在,而并未排除一个或多个其他特征、整数、步骤、组分或组的存在或添加。术语“包含”旨在包括由术语“基本上由……组成”和“由……组成”所涵盖的实施例。类似地,术语“基本上由……组成”旨在包含由术语“由……组成”所涵盖的实施例。
如本文所用,修饰所使用的成分或反应物的量的术语“约”是指数量上的变化,这些变化例如在真实世界中可能通过以下项可能发生:用于制成浓缩物或使用溶液的典型的测量和液体处理程序;这些程序中的非故意误差;用于制成组合物或进行方法的成分的制造、来源或纯度上的差异;等。
在存在的情况下,所有范围是包含端值的且可组合的。例如,当列举“1至5”的范围时,所列举的范围应解释为包括“1至4”、“1至3”、“1至2”、“1至2和4至5”、“1至3和5”等范围。
如本文所用,吸光度涉及物质吸收特定波长入射光的能力的测量。吸收度用来量化特定物质。
如本文所用,需氧条件涉及微生物在氧气存在的情况下生长的条件。
如本文所用,厌氧条件涉及微生物在氧气不存在的情况下生长的条件。
如本文所用,功效涉及所测试的化合物抑制H2S的能力。
如本文所用,计数板涉及通过接种含有新鲜培养基的板并连续稀释十倍来给出微生物样品的对数生长。然后将这些板培育一定时间。这有助于确定原始样品中存在的微生物数量。
如本文所用,恶劣环境涉及生命形态很难生存的极端条件的存在,例如,极高或极低的温度、极高或极低的压力、大气中高或低含量的氧气或二氧化碳;高水平的辐射、缺水;硫、石油和天然气的存在。井下油气应用是恶劣环境的实例。
如本文所用,抑制硫化氢(H2S)生产涉及在恶劣环境中通过有效处理策略来选择性抑制硫酸盐还原途径或控制硫酸盐还原细菌总数以将H2S水平降低大于或等于5%、可替代地大于或等于10%、可替代地大于或等于20%、可替代地大于或等于25%、可替代地大于或等于30%并且可替代地大于或等于50%。
如本文所用,光密度(OD)涉及吸光度的测量并且被定义为落在材料上的光强度与透射强度之比。
当参数以范围、优选范围、或一系列上限优选值和下限优选值给出时,这应当被理解为具体披露了由任何范围上限或优选值与任何范围下限或优选值的任何配对所形成的所有范围,而不论是否单独披露该范围。当本文列举数值范围时,除非另有说明,否则该范围旨在包括其端点、以及范围中的所有整数和分数。本发明的范围不旨在受限于如说明书中所述的特定值和实例。
本发明涉及用于抑制微生物对含硫化合物的还原反应的方法,该微生物在例如含有大于或等于10ppm硫化物的原油或含烃体系中产生硫化物。本发明强调了本文披露的N-羟基甲酰胺化合物用于在厌氧条件下抑制硫化物、特别是H2S。此方法可用于油气应用和井下油田储层。此组合物还可以应用于非油气应用中抑制其他有问题的细菌。
利用硫的原核生物可以通过还原硫酸盐、硫代硫酸盐、亚硫酸盐、亚硫酸氢盐、硫、其他无机硫化合物、有机硫化合物或其组合来产生硫化氢。利用硫的原核生物可以包括能够还原硫化合物以产生硫化物、硫化氢或硫化铁的细菌和古细菌的属或种。优选地,利用硫的原核生物可以包括硫酸盐还原细菌。取决于所用组合物的量和组合物中使用的N-羟基甲酰胺化合物的类型,硫化氢浓度可降低约25%至100%。表2列出了一些可用于作为本发明实施例披露的组合物中的化合物。
当本发明的抑制硫化物产生的方法时,该方法包括:
(i)提供包含至少一种具有结构1的化合物的组合物:
其中,Z:C(O)NHOH或C(Y)(R),
Y:氢,C6芳香族基团,C6杂芳香族基团,C6脂肪族环状基团或脂环族基团,杂基团如硝基、磷酸根、羟基,
R:以N-羟基甲酰胺、羧酸、醇或N-羟基甲酰胺封端的碳(n=1-10)直链或支链化合物,
X:氢、OH、NH2、卤素、碳(n=1-3)直链或支链化合物;以及
(ii)使该组合物与产生硫化物的细菌在厌氧条件下接触,以抑制硫化物产生。
此组合物优选是
且最优选包含
可替代地,本发明是一种抑制硫化物产生的方法,该方法包括:
(i)提供包含至少一种具有结构2的化合物的组合物:
其中,W:氢,任选地以羟基酰胺、羧酸、醇或N-羟基甲酰胺封端的碳(n=1-10)直链或支链化合物,C6芳香族基团,C6杂芳香族基团,C6脂肪族环状基团或脂环族基团;以及
(ii)使该组合物与产生硫化物的细菌在厌氧条件下接触,以抑制硫化物产生。
此组合物还可以包含:
在本文所述的方法中,优选使用这些组合物在含烃体系中抑制H2S产生,该含烃体系可以是井下、地下含烃地层、井、管道、流体分离容器、浮式生产储存容器、卸载容器、提炼厂、或储存系统。
在本文所述的方法中,组合物可以进一步与传统杀生物剂或其杀生物剂的组合一起施用,以在控制细菌方面产生协同作用。
这些组合物可以在像油气井下应用、地下含烃地层、功能流体、油气储层和生产系统、油气运输和储存系统、采矿、金属工业提取等恶劣环境中有效抑制H2S。此组合物还可以对存在于像冷却和加热系统、造纸和纸浆厂、膜和过滤系统中的非恶劣环境,以及存在于材料保存、废水处理中产生或使用的气体或液体、农场或屠宰场、垃圾填埋场、污水收集系统、市政废水厂、焦煤处理或生物燃料处理中的有问题的细菌有效。
实例
方法和分析
为了了解本发明的N-羟基甲酰胺化合物的硫化氢抑制功效,测试了若干种化合物。一些可以在此H2S抑制组合物中使用的化合物披露于表2中。关于测试程序,将这些化合物溶解于DMSO中,产生约8000-100000ppm的储备溶液,随后将其稀释于含有培养基和选定培养物的96孔板或10mL血清小瓶中,以在用于功效测试的最终溶液中产生的从0.09至1000ppm的不同浓度的化合物。
使用常见细菌的菌株来测试化合物的功效,即阿拉斯加脱硫弧菌(Desulfovibrioalaskensis)、长脱硫弧菌(Desulfovibrio longus)和加蓬脱硫弧菌(Desulfovibriogabonensis)。还通过使用标准方法来制备用于培养物的培养基。无菌使用培养物并将其在厌氧条件下培育。使用两种公认的方法测试各种化合物:
(1)96孔板法,和
(2)血清测试小瓶制备和硫化物测定
分别测试化合物C1、C12、C14、C15和C16,以了解它们中的每一种在标准温度和压力条件下抑制硫酸盐还原细菌产生H2S的功效。化合物C14和C15对硫化氢产生的功效在实例1、表6和表7中披露。可以注意到,这些化合物在减少H2S产生方面没有表现出显著的活性,并且因此,这些化合物对抑制H2S产生无效。
当在厌氧条件下在用于抑制硫化氢产生的组合物中使用时,化合物C12具有高功效。当以31.25至1000ppm的浓度范围、优选以125ppm至1000ppm的浓度范围使用时,该化合物显示出功效。实例1、表6和表7披露了所进行的实验的结果。
当在厌氧条件下在用于抑制硫化氢产生的组合物中使用时,化合物C16也具有高功效。当以125至1000ppm的浓度范围、优选以500ppm至1000ppm的浓度范围使用时,该化合物显示出功效。实例1、表6和表7披露了所进行的实验的结果。
出人意料地,在所测试的化合物中,当在厌氧条件下在用于抑制硫化氢产生的组合物中使用时,化合物C1示出最高功效。实例1、表3、表4和表5披露了所进行的实验的结果。当以0.2至205ppm的浓度范围、优选以1ppm至205ppm的浓度范围并且最优选以3ppm至205ppm的浓度范围使用时,该化合物显示出功效。
因此,为了进一步分析化合物C1的功效,在需氧条件和厌氧条件两者下使用另外的细菌培养物进行了对比测试。对于需氧条件测试,使用以下菌株:大肠杆菌(Escherichiacoli)、铜绿假单胞菌(Pseudomonas aeruginosa)、产气肠杆菌(Enterobacter aerogenes)和肺炎克雷伯菌(Klebsiella pneumoniae)。对于厌氧测试,使用以下菌株:肺炎克雷伯菌、产气肠杆菌、大肠杆菌和粪肠球菌(Enterococcus faecalis)。
发现与需氧条件相反,含有化合物C1的组合物在厌氧条件下优先抑制硫化氢产生。C1还示出通过使用代谢抑制来完全杀死各种微生物菌株的出人意料的功效。这些结果披露于实例2和实例3中。
实例1:化合物对硫酸盐还原细菌(SRB)的功效
储备溶液制备
从ChemBridge公司(ChemBridge Corporation)和西格玛奥德里奇公司(SigmaAldrich)购买化合物。通过将化合物以8000至100000PPM的浓度溶解于二甲亚砜(DMSO)来制备化合物的储备溶液。
表1:ATCC MB1250培养基的制备
储备培养物制备
将从ATCC接收的冻干的阿拉斯加脱硫弧菌14563、长脱硫弧菌51456和加蓬脱硫弧菌700201纯培养物分别重新悬浮在500ul的MB 1250中。将内容物无菌转移至5mL管的MB1250培养基中。将培养物在30℃的厌氧室中培育72hr。随后,通过添加等体积的培养物和50%甘油来制备最终浓度为25%甘油的单个储备培养物。然后将1ml培养物转移到2ml低温小瓶中,并在-80℃下储存。通过PCR、通过扩增16S rDNA区域来评估储备培养物的纯度,并且因此,验证了原始ATCC样品是纯培养物。在厌氧室中制备ATCC阿拉斯加脱硫弧菌14563、长脱硫弧菌51456和加蓬脱硫弧菌700201的48小时培养物。通过取1毫升(mL)纯培养物并接种9毫升(mL)新鲜MB1250培养基来将每个培养物制备为1:10培养物。阿拉斯加脱硫弧菌14563和加蓬脱硫弧菌700201均在30℃下生长,并且长脱硫弧菌51456培养物在35℃下生长。
处理准备
使用两种公认的方法测试各种化合物。
(1)96孔板法和(2)血清小瓶法。
(1)96孔板法(板制备和硫化物测定):
在48小时培育后,从每个培养物中取600微升(μL)并使用赛默飞世尔(Thermofisher)Spectronic 200分光光度计在600nm处测量光密度(OD)。将每个培养物在新鲜MB1250培养基中稀释至0.05OD600,并且添加到96孔板中。边缘孔由于其固有变异性和培养基的蒸发而没有使用。将每种组分以其对应的浓度添加,最终体积为每孔500μl。在表3、表6和表7中,列出用于实验的化合物的浓度。对于不同的处理和非处理对照,每个实验至少重复三次。从这些测试板中取出200μL,并放置在两个单独的板中持续3天和6天。然后将板用滴定盖(titer-top)密封,并放入厌氧箱中,并且在30℃的厌氧培育箱中培育。
在每个时间点,取出硫化物样品,并制作计数板。制作计数板以确定每个给药样品的对数增长。对于该过程,通过添加180μL含有0.01wt%硫酸亚铁铵的新鲜培养基MB1250来制备计数板。从挑战板(challenge plate)的每个孔中取出20μL,并使用20-200μL多通道移液管将其转移至计数板。使用多通道移液管将计数板混合三次,并将板连续稀释十倍(20μL至180μL)。对所有挑战板的行重复该连续稀释过程,得到总共6个计数板。
7天后,读取这些计数板。每个板中的硫酸亚铁铵将转化为硫化铁,这使SRB生长的孔从透明变为黑色。通过对一行中黑色孔的数量进行计数,可以确定挑战板中原始孔的对数增长。
为了确定每个样品中的硫化氢产量,还从每个挑战板中取出硫化物样品。对于每个孔,取出9μL,并且添加到60μL的具有0.02%乙酸的2%乙酸锌中。然后,添加180μL的milliQ水。将含有64%硫酸、<1%二甲基-4-苯二胺(DMPD)、水至100%的60μL储备溶液1添加到板上的每个孔中。这之后是3μL的含有50%氯化铁(III)的储备溶液3。所有化学品都是从飞世尔科技公司(Fisher Scientific)订购的,并如所收到的原样使用。将这些混合三次并在15分钟后读取。使用Biotek酶标仪在670nm处读取板。使用标准曲线将吸光度读数转换为mM,并使用硫的摩尔质量将其转换为百万分率。使用九水合硫化钠溶液绘制标准曲线,以得到0、0.125、0.25、0.5、1.0和1.5ppm的最终溶液。在670nm处读取后,绘制该数据给出了线性趋势线,其可用于确定样品的H2S水平。
(2)血清测试小瓶制备和硫化物测定
在48小时培育后,从阿拉斯加脱硫弧菌14563培养物中取600微升(μL)并使用赛默飞世尔(Thermofisher)Spectronic 200分光光度计在600nm处测量光密度(OD)。将每个培养物在新鲜MB1250培养基中稀释到0.05OD600,并且将5mL培养物溶液转移到10mL小瓶中,并且添加化合物C1的储备溶液。在表4和表5中,列出用于实验的化合物C1的浓度。对于不同的处理和非处理对照,每个实验至少重复三次。在2天、3天和5天时,制成计数板,并且以一式三份取出硫化物样品。
以一式三份进行计数,其具有从每个小瓶中取出的20μL,并将其放入96孔板中第一排的3个孔中。然后,使用如96孔板法中所述的测试板程序进行相同的连续稀释过程。在30℃的厌氧室中生长7天后读取这些计数。对于硫化物测定,从每个小瓶中取出9μL,并将其放置在具有2%乙酸锌的3个孔中。测定程序与96孔板法中描述的相同。
表2:代表性化合物的列表。
表3.使用96孔板法的化合物C1对SRB的H2S和对数(生长)功效。
表4.使用10mL血清小瓶法的化合物C1对阿拉斯加脱硫弧菌14563的H2S和对数(生长)功效。
**H2S测量期间的信号溢出。
表5.使用10mL血清小瓶法的化合物C1对阿拉斯加脱硫弧菌14563的H2S和对数(生长)功效。
表6.使用96孔板法的化合物对阿拉斯加脱硫弧菌14563的H2S和对数(生长)功效。
表7.使用96孔板法的化合物对阿拉斯加脱硫弧菌14563的H2S和对数(生长)功效。
实例2:化合物C1在需氧条件下对各种非SRB细菌的功效。
胰蛋白酶大豆肉汤(TSB)培养基的制备
通过将30克的BD Bacto胰蛋白酶大豆肉汤粉末(从飞世尔科技公司订购的)溶解于1升去离子水来制备胰蛋白酶大豆肉汤。以液体30次循环对其进行高压灭菌。使用的磷酸盐缓冲液是Hardy Diagnostics Dilu-Lok稀释瓶,并且从飞世尔科技公司接收。
需氧培养物制备
由ATCC大肠杆菌8739、铜绿假单胞菌15442、产气肠杆菌13048和肺炎克雷伯菌13883制成24小时培养物。通过取一环纯菌落并接种10mL的TSB来制备培养物。使这些在30℃下生长24小时。
测试板制备
在24小时培育后,从每个培养物中取600微升(μL)并使用赛默飞世尔Spectronic200分光光度计在600nm处测量光密度(OD)。每个培养物在新鲜磷酸盐缓冲盐水(PBS)中稀释至0.05OD600。使用这些培养物,制备两个深孔板。边缘孔由于其固有变异性和培养基的蒸发而没有使用。将每种组分以其对应的浓度添加,最终体积为每孔500μL。在表8中,列出用于实验的化合物C1的浓度。对于不同的处理和非处理对照,每个实验至少重复三次。然后将板用滴定盖密封并在30℃的培育箱中培育。
计数
在每个时间点进行计数,包括0小时、1小时、4小时和24小时。制作计数板以确定每个给药样品的对数增长。对于此过程,通过添加180μL新鲜TSB来制备计数板。从挑战板(challenge plate)的每个孔中取出20μL,并使用20-200μL多通道移液管将其转移至计数板。使用多通道移液管将计数板混合三次,并将板连续稀释十倍(20μL至180μL)。对所有挑战板的行重复该连续稀释过程,得到总共6个计数板。这些计数板在24小时后并且通过对一行中混浊孔的数量进行计数来读取。
表8.化合物C1在需氧条件下对各种非SRB细菌的对数(生长)功效。
实例3:化合物C1在厌氧条件下对各种非SRB细菌的功效。
储备培养物制备
肺炎克雷伯菌13883、产气肠杆菌13048、大肠杆菌8739和粪肠球菌29212培养物通过将来自冷冻库储备物的一个环添加到10mL新鲜酚红培养基中来制成。这些在30℃下厌氧生长24小时。
测试板制备
在24小时培育后,将每个培养物在新鲜酚红培养基中稀释到1:10。使用这些培养物,制备两个深孔板。边缘孔由于其固有变异性和培养基的蒸发而没有使用。将每种组分以其对应的浓度添加,最终体积为每孔250μL。在表9中,列出用于实验的化合物C1的浓度。对于不同的处理和非处理对照,每个实验至少重复三次。然后将板用滴定盖密封并在室温下在厌氧室中培育。在0小时、1小时、4小时和24小时进行计数。这些计数的过程与对实例2进行的过程相同;但使用酚红培养基代替胰蛋白酶大豆肉汤培养基。
表9.化合物C1在厌氧条件下在各种非SRB细菌中的对数(生长)功效。
Claims (7)
4.如权利要求1所述的方法,其中,所述组合物在油气井下应用、地下含烃地层、功能流体、油气储层和生产系统、油气运输和储存系统、采矿、金属工业提取、冷却和加热系统、造纸和纸浆厂、膜和过滤系统、材料保存、气体或液体生产、废水处理、农场或屠宰场、垃圾填埋场、污水收集系统、市政废水厂、焦煤处理或生物燃料处理中抑制硫化物产生。
7.如权利要求5所述的方法,其中,所述组合物在油气井下应用、地下含烃地层、功能流体、油气储层和生产系统、油气运输和储存系统、采矿、金属工业提取、冷却和加热系统、造纸和纸浆厂、膜和过滤系统、材料保存、气体或液体生产、废水处理、农场或屠宰场、垃圾填埋场、污水收集系统、市政废水厂、焦煤处理或生物燃料处理中抑制硫化物产生。
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