CN115768470A

CN115768470A - Total synthetic long-chain nucleic acids for vaccine production to protect against coronaviruses

Info

Publication number: CN115768470A
Application number: CN202180032734.6A
Authority: CN
Inventors: M·克里斯滕
Original assignee: Rocket Vaccine Co ltd
Current assignee: Rocket Vaccine Co ltd
Priority date: 2020-03-03
Filing date: 2021-03-03
Publication date: 2023-03-07
Also published as: IL296147A; EP4114452A1; MX2022010928A; AU2021231238A1; CA3170281A1; BR112022017733A2; KR20220150323A; JP2023517540A

Abstract

The present invention describes fully synthetic long-chain nucleic acids that can be used in biotechnological manufacturing methods to produce envelope proteins, viral envelopes and viral envelope fragments of SARS-CoV-2 and related coronaviruses in highly purified form as vaccines against COVID-19 and other viral diseases.

Description

Total synthetic long-chain nucleic acids for vaccine production to protect against coronaviruses

Description of the invention

The present invention relates to a fully synthetic long-chain nucleic acid according to independent claim 1. The invention also relates to a kit comprising two or more of these nucleic acids and to a biotechnological production unit comprising at least one plasmid comprising said nucleic acids. The invention also relates to viral envelopes, fragments of viral envelopes and/or viral envelope proteins obtainable by gene expression using said nucleic acids. Furthermore, the invention relates to a vaccine, in particular a vaccine against the coronavirus SARS-CoV-2, comprising a product obtainable by gene expression using this nucleic acid, and to a method for producing this vaccine.

The rapid development and availability of vaccines is crucial to combat many viruses and bacteria. The production of suitable vaccines is a multistage, complex process and, although often capital intensive, is not always successful. In general, the development of a suitable vaccine takes years. These long development times constitute a major problem, particularly with regard to emerging pathogens or mutated pathogens, since from an epidemiological point of view it is only possible to react too late, if at all, to the emergence of new diseases. In contrast, new or severely mutated pathogens can now be analyzed, identified, and further detected within weeks or even days, which is a great advance over the last century.

In this case, viruses are of particular interest because they have a high mutation rate, resulting in transmission from other species to humans. The rapid spread of these viruses makes them a major challenge in modern medicine. The current time between detection/identification of emerging viruses and development of vaccines is typically years (2020). In a few cases, with sufficient a priori knowledge, experimental vaccines can be provided within months. However, this time span is much longer than the typical time until thousands or millions of people are infected. This rapid spread is also a direct result of the high mobility of today's society.

Ideally, a sufficient number and highest quality of vaccine is available immediately after the identification of a new virus, and would allow nationwide vaccination of all people somehow close to the site of the initial outbreak of the new virus. Furthermore, an ideal method for such vaccines would be able to respond to the evolution and adaptation of the virus. This ideal production possibility appears to the person skilled in the art today to be utopia.

In the recent past, in particular, the coronavirus pandemic has significantly increased the relevance of developing suitable tools for vaccine production. It is agreed that the development of a vaccine against the coronavirus SARS-CoV-2 is the only effective means for long-term suppression of the epidemic situation and the associated global crisis.

Against this background, the object of the present invention was to provide a tool which allows the production of vaccines against the coronavirus SARS-CoV-2 in large quantities and with high quality.

This problem is solved by a fully synthetic long-chain nucleic acid according to claim 1. Preferred embodiments of the invention are reflected in the embodiments and the dependent claims.

Accordingly, the present invention relates in particular to the following embodiments:

1. a fully synthetic long-chain nucleic acid having at least 4,000 bases, characterized in that the nucleic acid comprises at least two of the four sequence portions A-D in any arrangement, wherein

i) Sequence part A comprises

a) A sequence as defined in seq.id.50 or a sequence having at least 98.5% sequence identity to a sequence as defined in seq.id.50; or

b) A sequence as defined in seq.id.3 or a sequence having at least 90% sequence identity to a sequence as defined in seq.id.3;

ii) sequence part B comprises

a) A sequence as defined in seq.id.48 or a sequence having at least 98.3% sequence identity to a sequence as defined in seq.id.48; or

b) A sequence as defined in seq.id.7 or a sequence having at least 90% sequence identity to a sequence as defined in seq.id.7;

iii) Sequence part C comprises

a) A sequence as defined in seq.id.49 or a sequence having at least 97.2% sequence identity to a sequence as defined in seq.id.49; or

b) The sequence defined in seq.id.11 or a sequence having at least 90% sequence identity to the sequence defined in seq.id.no.11

iv) sequence portion D comprises the sequence defined in seq.id.17 or a sequence having at least 98.5% sequence identity to the sequence defined in seq.id.17; or

Covering ribonucleic acid sequences corresponding to deoxyribonucleic acid sequences according to sequence parts a-D.

2. Nucleic acid according to embodiment 1, characterised in that it has at least 8'000 bases, preferably at least 20'000 bases in the defined sequence.

3. The nucleic acid according to one of the preceding embodiments, wherein the nucleic acid further comprises

a) 1.) the ORF1ab sequence defined by seq.id.51 or a sequence having at least 98.5% sequence identity to seq.id.51; or

2. ) i) the ORF1b sequence defined by seq.id.59 or a sequence having at least 98.5% sequence identity to seq.id.59; and

ii) the ORF1a sequence defined by SEQ.ID.58 or a sequence having at least 98.6% sequence identity to SEQ.ID.58.

b) The ORF3a sequence defined by seq.id.52 or a sequence having at least 99% sequence identity to seq.id.52; and

c) The sequence of ORF7a as defined by seq.id.54 or a sequence having at least 99.5% sequence identity to seq.id.54.

4. The nucleic acid according to embodiment 3, wherein the nucleic acid further comprises

a) An ORF6 sequence defined by seq.id.53 or a sequence having at least 94.1% sequence identity to seq.id.53; and/or

b) The ORF8 sequence defined by seq.id.55 or a sequence having at least 99% sequence identity to seq.id.55.

5. Nucleic acid according to one of the preceding embodiments, characterized in that the sequence portions a to C correspond to the sequence according to seq.id.19 or the corresponding ribonucleic acid sequence.

6. The nucleic acid according to any of the preceding embodiments, characterized in that the nucleic acid comprises at least three of the four sequence portions a-D in any arrangement or at least three of the four sequence portions having a ribonucleic acid sequence corresponding to a deoxyribonucleic acid sequence according to the sequence portions a-D.

7. Nucleic acid according to any one of the preceding embodiments, characterized in that the nucleic acid comprises four sequence portions a-D in any arrangement or four sequence portions having a ribonucleic acid sequence corresponding to the deoxyribonucleic acid sequence according to the sequence portions a-D.

8. Nucleic acid according to one of the preceding embodiments, characterized in that the nucleic acid further comprises at least one sequence consisting of:

SEQ.ID.15

SEQ.ID.28

seq id.29 and

SEQ.ID.30

or comprises one of the deoxyribonucleic acid sequences or the corresponding ribonucleic acid sequences according to said sequence portions seq.id.15, seq.id.28, seq.id.29 and seq.id.30.

9. Nucleic acid according to one of the preceding embodiments, characterised in that it has a maximum size of 1'000 bases, preferably a maximum size of 200'000 bases.

10. A vector comprising a nucleic acid according to one of the preceding embodiments.

11. A vector according to embodiment 10, wherein the vector comprises the sequence defined by seq.id.46 and seq.id.47.

12. The vector according to any one of embodiments 10 to 11, wherein the vector is a plasmid vector.

13. A kit comprising two or more nucleic acids according to any one of embodiments 1 to 9.

14. The kit according to embodiment 13, wherein the nucleic acid is present in at least one plasmid, preferably in two or more plasmids.

15. A biotechnological production unit comprising at least one vector according to embodiments 10 to 12.

16. A viral envelope, a viral envelope fragment and/or a viral envelope protein obtainable by gene expression using at least one nucleic acid according to any one of embodiments 1 to 9, using a vector according to any one of embodiments 10 to 12, using a kit according to any one of embodiments 13 or 14, or a biotechnological production unit according to embodiment 15, wherein the viral envelope, viral envelope fragment and/or viral envelope protein packages at least one nucleic acid according to any one of embodiments 1 to 9.

17. A vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to any of embodiments 1 to 9, and a product obtainable by gene expression using a vector according to any of embodiments 10 to 12, using a kit according to any of embodiments 13 or 14, in particular comprising a virus envelope, a virus envelope fragment and/or a virus envelope protein according to embodiment 16, using at least one nucleic acid according to any of embodiments 1 to 9 in a production organism.

18. A vaccine according to embodiment 17, comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1, b2, c1, c2, d1 or d2, wherein

(i) Protein component a comprises

a) A sequence according to seq.id.14 or a sequence having at least 90% sequence identity to seq.id.14 similar to the S protein of SARS-CoV-2; or

b) A sequence according to seq.id.18 or a sequence having at least 90% sequence identity to seq.id.18 similar to the S protein of SARS-CoV-2;

(ii) Protein component b1 comprises

a) A sequence according to seq.id.6 or a sequence having at least 90% sequence identity to seq.id.6 similar to envelope protein E of SARS-CoV-2; or

b) A sequence according to seq.id.21 or a sequence having at least 90% sequence identity to seq.id.21 that is similar to envelope protein E of SARS-CoV-2; and

protein component b2 comprises a sequence according to seq.id.8 similar to the envelope protein E of MHV59A, or an equivalent protein (equivalent protein) comprising a sequence having at least 90% sequence identity to seq.id.8;

(iii) Protein component c1 comprises

a) A sequence according to seq.id.10 or a sequence having at least 90% sequence identity to seq.id.10 similar to envelope protein M of SARS-CoV-2; or

b) A sequence according to seq.id.22 or a sequence having at least 90% sequence identity to seq.id.22 similar to the membrane protein M of SARS-CoV-2; and

protein component c2 comprises a sequence according to seq.id.12 similar to MHV59A membrane protein M, or an equivalent protein comprising a sequence having at least 90% sequence identity to seq.id.12; and

(iv) Protein component d1 comprises

a) A sequence according to seq.id.2 or a sequence having at least 90% sequence identity to seq.id.2 similar to nucleocapsid phosphoprotein N of SARS-CoV-2; or

b) A sequence according to seq.id.26 or a sequence having at least 90% sequence identity to seq.id.26 similar to nucleocapsid phosphoprotein N of SARS-CoV-2; and

protein component d2 comprises a sequence according to seq.id.4 similar to the nucleocapsid phosphoprotein N of MHV59A or an equivalent protein comprising a sequence having at least 90% sequence identity to seq.id.no. 4.

19. A method for producing a vaccine against coronavirus SARS-CoV-2, comprising the following successive steps:

a) The nucleotide sequence according to one of embodiments 1 to 9 is introduced into a biotechnological production unit, in particular a cell line,

wherein a nucleic acid-based mRNA encoding at least two protein components selected from the group consisting of protein components a, b1, b2, c1, c2, d1 or d2 is prepared by translation;

b) Obtaining a protein fraction from the biotechnological production unit in step a); and

c) Purifying the obtained protein fraction to obtain a vaccine against coronavirus SARS-CoV-2.

20. A method for producing a vaccine against coronavirus SARS-CoV-2, said vaccine comprising a viral envelope, a viral envelope fragment and/or a viral envelope protein according to embodiment 16, said method comprising the following successive steps:

a) Introducing a nucleotide sequence according to one of embodiments 1 to 9 into a biotechnological production unit, wherein the biotechnological production unit comprises nucleotides encoding at least one protein component selected from protein components a, b1, c1 and d 1;

b) Obtaining a viral envelope fragment and/or a viral envelope protein from the biotechnological production unit in step a); and

c) Purifying the obtained protein fraction to obtain a vaccine against coronavirus SARS-CoV-2, said vaccine comprising a viral envelope, a viral envelope fragment and/or a viral envelope protein according to embodiment 16.

21. A method for producing a vaccine against coronavirus SARS-CoV-2, comprising the following successive steps:

a) Introducing a vector according to one of embodiments 10 to 12 into an amplification biotechnological production unit;

b) Amplifying the nucleotides according to one of embodiments 1 to 9 in an amplification biotechnological production unit;

c) Obtaining the nucleotides amplified in step b);

d) A vaccine against coronavirus SARS-CoV-2 is obtained by using the method of embodiment 19 or 20.

Thus, the present invention relates to a fully synthetic long-chain nucleic acid having at least 4,000 bases, characterized in that the nucleic acid comprises at least two of the four sequence portions a-D in any arrangement, wherein i) sequence portion a comprises a) the sequence defined in seq.id.1 or a sequence having at least 98.5% sequence identity to the sequence defined in seq.id.1; or b) the sequence defined in seq.id.3 or a sequence having at least 90% sequence identity to the sequence defined in seq.id.3; ii) sequence part B comprises a) the sequence defined in seq.id.5 or a sequence having at least 98.3% sequence identity to the sequence defined in seq.id.5; or b) the sequence defined in seq.id.7 or a sequence having at least 90% sequence identity to the sequence defined in seq.id.7; iii) Sequence part C comprises a) the sequence defined in seq.id.9 or a sequence having at least 97.2% sequence identity to the sequence defined in seq.id.9; or b) the sequence defined in seq.id.11 or a sequence having at least 90% sequence identity to the sequence defined in seq.id.11; iv) sequence portion D comprises the sequence defined in seq.id.13 or a sequence having at least 98.5% sequence identity to the sequence defined in seq.id.13; or cover a ribonucleic acid sequence corresponding to a deoxyribonucleic acid sequence according to sequence parts a-D.

The nucleic acids according to the invention allow to significantly speed up the production of the above-mentioned vaccines and to produce well-defined vaccines with a very high specificity for viruses or modifications, in particular for the coronavirus SARS-CoV-2.

As will be shown further below, the specific sequence characteristics of the sequence portions comprised in the nucleic acid sequences according to the invention allow the nucleic acids to be produced completely synthetically and thus to be tailored. Thus, the nucleic acid according to the invention differs from nucleic acids naturally occurring in coronaviruses not only in that it is in certain embodiments not only DNA instead of RNA, but also in the following sequences: which, in contrast to naturally occurring sequences, allows the complete synthetic production of nucleic acids by means of chemical synthesis.

Finally, the nucleic acids according to the invention thus make it possible to express protein components defined with molecular accuracy. Thus, when these protein components are administered as a vaccine, optimal immunity can be obtained in the vaccine recipient. At the same time, the risk of possible side effects, which are highly prevalent in the case of inaccurately defined protein components, is greatly minimized. Furthermore, the fact that the protein components can be produced using common expression systems for protein expression means that vaccines can be obtained in large quantities very quickly. This is crucial for viruses such as the coronavirus SARS-CoV-2, whose spread has reached a degree of pandemic, and therefore extensive administration of vaccines is required to be suppressed.

The following terms and concepts shall be used in the context of the present invention:

the term "nucleic acid" refers to DNA, RNA, and any modifications thereof. The nucleic acid may be single-stranded or double-stranded. Modifications include, but are not limited to, those that provide other chemical groups that incorporate additional charge, polarizability, hydrogen bonding, electrostatic interactions, and mobility into the nucleic acid ligand base or the nucleic acid ligand as a whole. Such modifications include, but are not limited to, modifications of the sugar at the 2' -position, modifications of the pyrimidine at the 5-position, modifications of the purine at the 8-position, modifications at exocyclic amines, substitutions of 4-thiouridine, substitutions of 5-bromo-or 5-iodo-uracil; backbone modifications, methylation, unusual base pairing combinations, such as the isobase isocytidine and isoguanidine. Modifications may also include 3 'and 5' modifications, such as capping.

And (3) fully synthesizing. From a chemical point of view, nucleic acids are very complex molecules with repeating units (so-called bases). In this context, the term "fully synthetic" means that the nucleic acid according to the invention is produced by a series of chemical reaction steps using chemical reagents. Biochemical aids, such as enzymes, can also be used during various later production steps, such as the ligation of already longer oligomers. Already longer oligomers may optionally also be synthesized. The fully synthetic nucleic acids have sequence characteristics that enable chemical production processes and differ from naturally occurring nucleic acids by one or more of the following sequence characteristics:

i) The absence of one or more enzyme restriction sites, particularly restriction sites for type IIS restriction endonucleases known to those skilled in the art;

ii) the absence or reduced occurrence of repetitive nucleic acid sequences having more than 9 contiguous units of the same base within the fully synthetic nucleic acid as compared to the corresponding naturally occurring nucleic acid;

iii) The absence or reduced occurrence of a repeating base pair sequence of more than 12 bases as compared to a corresponding naturally occurring nucleic acid;

iv) the absence or reduced occurrence of indirectly repeated base pair fragments, relative to the corresponding naturally occurring nucleic acid, said fragments consisting of more than 12 base units known to those skilled in the art as their reverse complement;

v) the absence or reduced occurrence of nucleic acid sequences known to the person skilled in the art having more than 9 consecutive repeats of a double base unit (dinucleotide repeats) relative to the corresponding naturally occurring nucleic acid; and

vi) the occurrence of a nucleic acid sequence known to the person skilled in the art having more than 5 consecutive repeats of a three-base unit (trinucleotide repeat) does not occur or is reduced relative to the corresponding naturally occurring nucleic acid.

In some embodiments, fully synthetic nucleic acids and/or comprising sequence features are produced, in part, according to the methods described in Venetz, J.E. et al, 2019, proceedings of the National Academy of sciences,116 (16), 8070-8079 and/or the SI appendix thereof.

In some embodiments, the fully synthetic nucleic acid comprises sequence features that enable a chemical production process and differs from naturally occurring nucleic acids by two or more of the above-described sequence features, in particular the above-described sequence features i) -vi).

In some embodiments, the fully synthetic nucleic acid comprises sequence features that enable a chemical production process and differs from naturally occurring nucleic acids by three or more of the above-described sequence features, in particular the above-described sequence features i) -vi).

In some embodiments, the fully synthetic nucleic acid comprises sequence features that enable chemical production processes and differs from naturally occurring nucleic acids by four or more of the above-described sequence features, in particular the above-described sequence features i) -vi).

In some embodiments, the fully synthetic nucleic acid comprises sequence features that enable a chemical production process and differs from naturally occurring nucleic acids by five or more of the above-described sequence features, in particular the above-described sequence features i) -vi).

In some embodiments, the fully synthetic nucleic acid comprises sequence features that enable chemical production processes and differs from naturally occurring nucleic acids by six of the above-described sequence features, in particular the above-described sequence features i) -vi). Long-chain oligonucleotides have been commercially available for many years in short fragments, typically producing fragments with 60, 100 or 200 bases. Large numbers of longer oligonucleotides are not readily available because the syntheses currently used have too high an error rate to produce reasonable amounts of longer nucleic acids. Thus, such fragments having less than 1000 bases are referred to as short strands, while nucleic acids having 1000 bases or more are referred to as long strands. Long-chain nucleic acids having 1000 to 5000 bases can be produced at considerable expense at present (for example, by Twist Bioscience, life-Technologies). Long-chain nucleic acids with more than 5000 bases are extremely complex but chemically well-defined molecules. Each molecule can be fully described by position, type and attachment to other parts of the molecule according to classical organic chemistry. Thus, two identical long-chain nucleic acids are identical, despite their size and despite the fact that they contain tens of thousands to millions of atoms, since all components are identical and identically linked.

Interpretation of the terminal groups, any residues of protecting groups or other auxiliary agents from nucleic acid synthesis. The above description relates to the type of bases in the nucleic acid. The person skilled in the art knows that the synthesis is carried out by means of various auxiliaries which are cut off at the end. However, sometimes residues of such groups remain, or other parts of the molecule are derivatized before or after the synthetic step. Such groups are known to those skilled in the art and include, inter alia, poly-a tails, modified DNA bases, cleavable linkers from solid phase synthesis, biochemical groups such as biotin or streptavidin, and the like.

Other possible modifications and modifications used in standard methods involve fluorescent markers. These modifications or their residues should not affect the above description, and a set of identical nucleic acids should be considered identical if all n bases at each position and at the position of the base type are identical for all bases. In other words, the nucleic acids of the present invention also include nucleic acids having the above-described modifications or residues, provided that they have the base sequences required for the present invention.

Thus, according to a first aspect, the present invention relates to nucleic acids having specific properties. These specific properties are included in the base sequence (i.e., sequence) and are only obtained when the nucleic acid of the present invention has certain properties. These properties are directly a partial or complete chemical description of a particular molecule. However, for simplicity, the base sequence should be indicated in the present specification herein, and it is clearly indicated to refer to a particular molecule throughout. Thus, the base sequence is only a practical form of description and is clearly more suitable than the textual representation of the molecule or its IUPAC name for the present invention.

The molecules of the invention acquire specific properties by virtue of the presence of specific sequences, similar to the description of a group of classical chemical reagents having one or more molecular moieties, which are often chemically abbreviated as "R" and which can be described in more detail later by the description of "R". Thus, in the present invention, similar to this general method in organic chemistry, a set of sequences is described which are responsible for the specific properties of the long-chain fully synthetic nucleic acids of the invention.

The nucleic acids of the invention are characterized by the fact that: they contain fully synthetic nucleic acids encoding at least two of the 4 types of proteins of the envelope protein coronavirus.

As used herein, the term "type of coronavirus envelope protein" refers to group a, group B, group C, or group D proteins of coronaviruses. As used herein, the term "group a" proteins refers to the nucleocapsid protein (N-type) group of coronaviruses. As used herein, the term "group B" refers to the group of envelope proteins (E-type) of coronaviruses. As used herein, the term "group C" protein refers to the membrane protein (M-type) of coronaviruses. As used herein, the term "group D" protein refers to the glycosylated surface protein (S-type) of coronaviruses.

In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one histone a and at least one histone B. In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one group a protein and at least one group C protein. In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one histone a and at least one histone D. In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one histone B and at least one histone C. In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one histone B and at least one histone D. In some embodiments, the nucleic acids described herein are characterized by the fact that they comprise nucleic acids encoding at least one histone C and at least one histone D.

In some embodiments, the nucleic acids of the invention are characterized by the fact that: they can use

(a) Comprising more than 4,000 bases in a well-defined sequence; and

(b) Comprising at least 2 of 4 particularly important sequences, said 4 particularly important sequences being assigned to 4 sequence groups A-D which code for envelope proteins of 4 types of coronaviruses, wherein

i) The first sequence group A encodes envelope protein of coronavirus nucleocapsid protein N,

ii) a second sequence group B encodes an envelope protein of the coronavirus envelope protein type E,

iii) The third sequence group C encodes an envelope protein of coronavirus membrane protein type M, and

iv) the fourth sequence group D encodes the envelope protein of the coronavirus glycosylated surface protein S.

The sequence part a disclosed in the present specification comprises a sequence according to seq.id.1 or seq.id.3 encoding a corresponding protein sequence according to seq.id.2 or seq.id.4. In some embodiments, sequence portion a comprises the sequence defined by seq id.50 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.50.

In some embodiments, sequence portion a comprises a sequence having at least 90% sequence identity to seq.id.3.

In some embodiments, sequence portion a comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.2.

In some embodiments, sequence portion a comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.4.

In some embodiments, sequence portion a comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.2 and seq.id.4.

The sequence part B disclosed in the present specification comprises a sequence according to seq.id.5 or seq.id.7 encoding a corresponding protein sequence according to seq.id.6 or seq.id.8. In some embodiments, sequence portion B comprises the sequence defined by seq.id.48 or a sequence having at least 98.3%, at least 98.6%, at least 99.1%, or at least 99.5% sequence identity to seq.id.48.

In some embodiments, sequence portion B comprises a sequence having at least 90% sequence identity to seq.id.7.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.6.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.8.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% of seq id.6 and seq id.8.

The sequence part C disclosed in the present specification comprises a sequence according to seq.id.9 or seq.id.11 encoding the sequence of the corresponding protein according to seq.id.10 or seq.id.12. In some embodiments, sequence portion C comprises the sequence defined by seq id.49 or a sequence having at least 97.2%, at least 97.4%, at least 97.6%, at least 97.8%, at least 98%, at least 98.2%, at least 98.4%, at least 98.6%, at least 98.8%, at least 99%, at least 99.2%, at least 99.4%, at least 99.6%, at least 99.8% sequence identity to seq id.49.

In some embodiments, sequence portion C comprises a sequence having at least 90% sequence identity to seq.id.11.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.id.12.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.10.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.10 and seq.id.12.

The sequence part D disclosed in the present specification comprises a sequence according to seq.id.13 encoding a corresponding protein sequence according to seq.id.14. In some embodiments, sequence portion D comprises the sequence defined by seq id.17 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.17.

In some embodiments, sequence portion B comprises a sequence encoding an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.id.14.

The term "percent (%) sequence identity" with respect to a reference sequence is defined as the percentage of nucleotides or amino acid residues in a candidate sequence that are identical to the nucleotides or amino acid residues in the reference sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for the purpose of determining percent amino acid sequence identity can be accomplished in a variety of ways within the skill in the art, for example, using publicly available computer software such as BLAST, BLAST-2, ALIGN, or Megalign (DNASTAR) software. One skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms necessary to achieve maximum alignment over the full length of the sequences being compared.

In some embodiments, the nucleotide sequence of the invention is altered (e.g., to facilitate the production process of the nucleotide sequence or its product) without altering or substantially altering the properties of the protein product.

In some embodiments, the alteration of the nucleotide sequence of the present invention comprises at least one alteration selected from the group consisting of:

1) Base substitutions, insertions or deletions relative to a reference sequence without altering or substantially altering the properties of the protein product;

2) Replacing codons with a synonymous version; and

3) Reducing the number of putative genetic elements present within a protein coding sequence, such as (alternative) ORFs, predicted internal transcription start sites within a gene, and/or sequence motifs (predicted or hidden) that fine-tune translation rates (e.g., ribosome retention motifs).

Testing whether a gene of the altered nucleotide sequence of the invention remains functional will identify genes in which additional information beyond the amino acid code is essential for normal function.

In some embodiments, the nucleotide sequences described herein are altered to improve the biological function of the encoded protein product.

Such biological functions include, but are not limited to, stability enhancement, production promotion (e.g., insertion of additional replication initiation sequences), replication restriction.

In some embodiments, the nucleotide sequences described herein are altered to encode at least one alternative protein of interest having a similar structure but having an alternative biological function (e.g., the function of the protein of the mutant virus).

One skilled in the art can obtain such altered nucleotide sequences by analyzing the sequence encoding at least one alternative protein of interest (e.g., the nucleotide sequence of a mutant virus) and implementing the relevant alterations (e.g., mutations) into the most similar nucleotide sequences described herein. In some embodiments, the nucleotide sequence of the most similar nucleotide sequence described herein is the sequence defined by seq.id.1, seq.id.3, seq.id.5, seq.id.7, seq.id.9, seq.id.11, seq.id.13 and/or seq.id.17.

In some embodiments, the nucleotide sequence of the most similar nucleotide sequence described herein is the sequence defined by seq.id.1, seq.id.5, seq.id.9, seq.id.13.

In some embodiments, the coronavirus described herein is SARS-CoV-2. In some embodiments, SARS-CoV-2 described herein is a SARS-CoV-2 variant selected from: lineage b.1.1.207, lineage b.1.1.7, cluster 5, 501.V2 variant, lineage p.1, lineage b.1.429/cal.20c and lineage b.1.525.

In some embodiments, SARS-CoV-2 described herein is a SARS-CoV-2 variant described by the Nextstrain clade selected from the group consisting of 19A, 20C, 20G, 20H, 20B, 20D, 20F, 20I, and 20E.

In some embodiments, the sequence encoding the at least one surrogate protein of interest comprises a sequence encoding at least one protein characteristic of a SARS-CoV-2 variant. In some embodiments, the protein characteristic of at least one SARS-CoV-2 variant is a protein encoded by a sequence having at least 90%, having at least 91%, having at least 92%, having at least 93%, having at least 94%, having at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to sequence seq.id.18, seq.id.21, seq.id.22, and/or seq.id.26.

Such implementation of relevant changes may be achieved, for example, by insertion, deletion, substitution and/or modification of at least one base, but not more than a percentage of the nucleotide sequence described herein.

In some embodiments, the nucleotide sequence of the most similar nucleotide sequence described herein is the sequence defined by seq.id.1, seq.id.3, seq.id.5, seq.id.7, seq.id.9, seq.id.11, seq.id.13 and/or seq.id.17.

In some embodiments, the nucleotide sequence of the most similar nucleotide sequence described herein is at least one sequence defined by seq.id.1, seq.id.5, seq.id.9 and/or seq.id.13.

In some embodiments, insertions, deletions, or modifications can be achieved by de novo synthesis of a nucleic acid of the invention using a series of chemical reaction steps using chemical reagents as described herein.

The altered sequence may comprise sequence features (e.g., sequence features i) -vi) described above) which are capable of and/or improve the chemical production process of the altered sequence at more or different positions than the nucleotide sequence defined by seq.id.1, seq.id.3, seq.id.5, seq.id.7, seq.id.9, seq.id.11, seq.id.13 and/or seq.id.17.

Their possible conversion into IUPAC classifiable molecules is known to those skilled in the art. As an alternative to deoxyribonucleic acids as defined above, corresponding ribonucleic acids may also be present. In other words, in addition to the deoxyribonucleic acid sequences according to the sequence portions A-D, the corresponding ribonucleic acid sequences are also included according to the definition of the invention. Wherein the corresponding ribonucleic acid has a sequence portion as defined above, wherein thymine (T) is replaced by uracil (U).

The base pair sequences of the long-chain nucleic acids of the invention which code for the envelope proteins E, M, N and S of MHV and SARS-CoV-2 and, if applicable, for the RNA-dependent RNA polymerase of MHV represent a result of a complex development, where, in a first step, a large number of sequence variants are formed by calculation starting from the natural amino acid sequence of the corresponding protein, taking into account the redundancy of the genetic code.

In particular, the base pair sequence of the long-chain nucleic acids of the invention which code for the SARS-CoV-2 proteins E, M, N and/or S represents a complex development in which, in a first step, a large number of sequence variants are formed by calculation starting from the natural amino acid sequence of the corresponding protein, taking into account the redundancy of the genetic code.

In a second step, the base pair sequence of each encoded envelope protein is determined from the resulting sequence tree, which firstly most closely resembles the natural sequence in terms of biological function and secondly also has the best sequence characteristics to be able to carry out the chemical production process.

In addition, the sequences encode a combination of structural proteins of the wild-type virus. This enables the immune system to obtain a wide range of epitopes, including T cell epitopes (see, e.g., grifeni, a., et al, 2020, cell,181 (7), 1489-1501). This broad epitope enables immunity against a broad range of viral variants in patients with or without pre-existing immunity.

Accordingly, the present invention is based, at least in part, on the following findings: the nucleic acids of the invention are capable of efficiently producing combined virus-like proteins with limited replication capacity but similar antigenic effect as the original virus.

As mentioned above, the nucleic acid according to the invention has at least 4'000 bases or base pairs. Preferably it has at least 8'000 bases, particularly preferably at least 20'000 bases in the defined sequence. The maximum size of nucleic acid is preferably 1'000 bases, and the maximum size is preferably 200'000 bases.

It has been shown repeatedly that large sequences are difficult to produce, amplify and/or express, but a large number of bases facilitates consistent production of certain combinations of virus-like proteins with similar antigenic effects as the original virus.

The tools and methods provided herein enable the production of nucleic acids according to the invention over a range of lengths (see, e.g., examples 1-3).

Accordingly, the present invention is based, at least in part, on the following findings: the nucleic acids of the invention, which are in a range of lengths, are effective in producing combined virus-like proteins with limited replication capacity but similar antigenic effect as the original virus.

The nucleic acids according to the invention may be present as single long-chain nucleic acids or divided into individual long-chain nucleic acids.

In some embodiments, the nucleic acid according to the invention may be present as a single long-chain nucleic acid or divided into up to 4 individual long-chain nucleic acids.

Separation into separate long-chain nucleic acids can facilitate amplification of the nucleic acids of the invention (example 3).

According to another preferred embodiment, the sequence portions A-D are arranged according to the sequence SEQ. EQ.16.

It is also preferred that sequence part D consists of seq.id.17 and encodes a protein sequence according to seq.id.18.

According to another preferred embodiment, the sequence portions a-C are arranged according to the sequence seq.id.19, whereby sequence portion a encodes a protein sequence according to seq.id.26, sequence portion B encodes a protein sequence according to seq.id.21, sequence portion C encodes a protein sequence according to seq.id.22 and, furthermore, the sequence portions a-C can be extended with sequences encoding seq.id.20, seq.id.22, seq.id.23, seq.id.24, seq.id.25 and seq.id.27.

In some embodiments, the invention relates to a nucleotide sequence according to the invention, wherein the nucleotide sequence is defined by a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq.id.19 or a corresponding ribonucleic acid sequence.

It is particularly preferred to supplement the sequence parts a-D disclosed in the present specification with a nucleic acid sequence of sequence part E comprising a sequence according to seq.id.15 or seq.id.30, a polyprotein sequence according to seq.id.31 and seq.id.32 of an RNA-dependent RNA polymerase of a coronavirus.

The sequences according to seq.id.15 or seq.id.30 may represent components of the nucleic acid according to the invention and are thus present in the same molecule in combination with two or more sequences of sequence parts a-D. It is also conceivable that it is present in the kit as a component of a separate molecule together with the nucleic acid according to the invention. Possible transfers into IUPAC classifiable molecules are known to those skilled in the art.

The presence of sequence part E is relevant if RNA is introduced into the biotechnological production unit instead of the DNA plasmid for gene expression of the corresponding protein. In this connection, it is also conceivable to introduce the sequence portion E into the kit in the form of an RNA according to seq.id.33 or that seq.id.34 is present in the kit. This will be further explained in the context of the specific embodiments below.

These particular sequences have been shown to be particularly advantageous, firstly in terms of their similarity to the native sequence or their biological function, and secondly in chemical production processes.

According to another preferred embodiment, the nucleic acid comprises at least three of the four sequence portions a-D in any arrangement. In this respect, it is particularly preferred that the nucleic acid comprises four sequence portions A-D in any arrangement.

Furthermore, it is preferred that the nucleic acid further comprises at least one sequence consisting of:

SEQ.ID.15

SEQ.ID.28

seq.id.29 and

SEQ.ID.30。

in some embodiments, the nucleic acid of the invention comprises a deoxyribonucleic acid sequence or a corresponding ribonucleic acid sequence according to the sequence parts seq.id.15, seq.id.28, seq.id.29 and seq.id.30.

In some embodiments, the invention relates to a nucleic acid according to the invention, characterized in that the nucleic acid comprises seq.id.28, or a corresponding ribonucleic acid sequence.

In some embodiments, the invention relates to a nucleic acid according to the invention, characterized in that the nucleic acid comprises seq.id.29, or a corresponding ribonucleic acid sequence.

In some embodiments, the invention relates to a nucleic acid according to the invention, characterized in that the nucleic acid comprises seq.id.8 and seq.id.29, or the corresponding ribonucleic acid sequences.

The nucleic acids of the invention have the special property that they can be incorporated into cell lines or other production organisms by standard methods and stimulate the production of fragments or complete envelopes of viruses. Standard methods required for this purpose are known to the person skilled in the art and are described in the context of specific embodiments.

The inventors have found that although some of the ORFs believed to be useful for efficient replication of the original virus are omitted, the viral particles can be amplified and subsequently translated and successfully assembled. The resulting viral particles are still able to infect cells and induce the production of non-infectious viral fragments.

The inventors found that ORF6 and ORF8 of SARS-CoV-2 viral genome (see FIG. 5) can be omitted or deleted and viral assembly is still possible.

In some embodiments, the invention relates to a nucleic acid according to the invention, wherein the nucleic acid further comprises

a) 1.) the ORF1ab sequence defined by seq id.51 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.51; or

2. ) i) the ORF1b sequence defined by seq id.59 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.59; and

ii) the ORF1a sequence defined by seq id.58 or a sequence having at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.58;

b) An ORF3a sequence defined by seq id.52 or a sequence having at least 99%, at least 99.1%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.52; and

c) The sequence of ORF7a defined by seq.id.54 or a sequence having at least 99.5% sequence identity to seq.id.54.

In some embodiments, the invention relates to a nucleic acid according to the invention, wherein said nucleic acid further comprises

a) 1.) the ORF1ab sequence defined by seq.id.51 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.51; or

ii) the ORF1a sequence defined in seq id.58 or a sequence having at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq id.58;

b) An ORF3a sequence defined by seq id.52 or a sequence having at least 99%, at least 99.1%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.52;

c) The ORF7a sequence defined by seq.id.54 or a sequence having at least 99.5% sequence identity to seq.id.54; and

d) The ORF8 sequence defined by seq.id.55 or a sequence having at least 99%, at least 99.3% or at least 99.6% sequence identity to seq.id.55.

b) The ORF3a sequence defined by seq id.52 or a sequence having at least 99%, at least 99.1%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.52;

d) The ORF6 sequence defined by seq id.53 or a sequence having at least 94.1%, at least 94.7%, at least 95.2%, at least 95.8%, at least 96.3%, at least 96.8%, at least 97.4%, at least 97.9% or at least 98.5%, at least 99% or at least 99.6% sequence identity to seq id.53.

c) The ORF7a sequence defined by seq.id.54 or a sequence having at least 99.5% sequence identity to seq.id.no. 54;

d) An ORF6 sequence defined by seq id.53 or a sequence having at least 94.1%, at least 94.7%, at least 95.2%, at least 95.8%, at least 96.3%, at least 96.8%, at least 97.4%, at least 97.9%, at least 98.5%, at least 99%, or at least 99.6% sequence identity to seq id.53; and

e) The ORF8 sequence defined by seq.id.55 or a sequence having at least 99%, at least 99.3% or at least 99.6% sequence identity to seq.id.55.

In some embodiments, the invention relates to a nucleic acid according to the invention, wherein the nucleic acid further comprises 3'UTR, 5' UTR, TRS-L, TRS-B: S, TRS-B: ORF3a, TRS-B: E, TRS-B: M, TRS-B: ORF6, TRS-B: ORF7a, TRS-B: ORF8 and/or TRS-B: N.

In some embodiments, the invention relates to a nucleic acid according to the invention, wherein the nucleic acid further comprises a 3'utr defined by seq.id.57 and/or a 5' utr defined by seq.id.56.

In some embodiments, the invention relates to a nucleic acid according to the invention, wherein the nucleic acid further comprises a TRS-L, TRS-B: S, TRS-B: ORF3a, TRS-B: E, TRS-B: M, TRS-B: ORF6, TRS-B: ORF7a, TRS-B: ORF8 and/or TRS-B: N defined by the sequence ACGAAC.

In some embodiments, the nucleic acid sequence comprises the sequence defined by seq id.41 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.41.

In some embodiments, the nucleic acid sequence comprises the sequence defined by seq id.42 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.42.

In some embodiments, the nucleic acid sequence comprises the sequence defined by seq id.43 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.43.

In some embodiments, the nucleic acid sequence comprises the sequence defined by seq id.44 or a sequence having at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.44.

In some embodiments, the nucleotide sequence described herein refers to the corresponding ribonucleic acid sequence.

ORF6 and ORF8 of SARS-CoV-2 inhibit the type I interferon signaling pathway (Li, J.Y. et al, 2020, virus research,286, 198074) and thus block an adequate immune response. Thus, deletion or omission of the sequence of ORF6 and/or ORF8 of SARS-CoV-2 in the vector not only limits the replicative nature of the encoded viral particle, but also increases its antigenicity.

Accordingly, the present invention is based, at least in part, on the following findings: the nucleotide sequences of the present invention encode viral particles or portions thereof that have surprising antigenicity and limited replication ability.

In some embodiments, the nucleic acid sequences of the invention are vectors or portions of vectors.

As used herein, the term "vector" refers to a nucleic acid molecule capable of transferring or transporting itself and/or another nucleic acid molecule into a cell. The transferred nucleic acid is typically linked to, i.e., inserted into, a carrier nucleic acid molecule. The vector may comprise sequences that direct autonomous replication in the cell, or may comprise sequences sufficient to allow integration into the DNA of the host cell. In some embodiments, the vector described herein is a vector selected from the group consisting of a plasmid (e.g., a DNA plasmid or an RNA plasmid), a shuttle vector, a transposon, a cosmid, a bacterial artificial chromosome, and a viral vector.

In certain embodiments, the invention relates to a vector according to the invention, wherein the vector does not comprise sequence part B and the regulation of sequence part a does not comprise at least one accessory protein.

In certain embodiments, the present invention relates to a vector according to the present invention, wherein the vector is a plasmid vector.

In some embodiments, the plasmid vectors described herein have a selectable marker and a sequence that determines the origin of replication. In some embodiments, the invention relates to a vector according to the invention, wherein the vector comprises the sequence defined by seq.id.46 and seq.id.47.

In some embodiments, the present invention relates to a vector according to the present invention, wherein the vector comprises at least one sequence encoding a RNA polymerase promoter and at least one untranslated region comprising a sequence capable of synthesizing a negative strand RNA and/or capable of synthesizing a positive strand RNA.

In some embodiments, the present invention relates to a vector according to the present invention, wherein the vector comprises at least one sequence encoding a T7 promoter and at least two untranslated regions comprising sequences capable of synthesizing negative strand RNA and/or capable of synthesizing positive strand RNA.

In some embodiments, the present invention relates to a vector according to the present invention, wherein the vector comprises at least one sequence encoding the T7 promoter as defined in seq.id.28 and at least two untranslated regions comprising sequences according to seq.id.56 and 57.

In some embodiments, the invention relates to a vector according to the invention, wherein the vector is a plasmid vector.

In some embodiments, the invention relates to a vector according to the invention, wherein the vector comprises the sequence defined in seq.id.45.

In some embodiments, the nucleotide sequence described herein comprises a sequence having at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to seq.id.45.

In some embodiments, the vectors described herein comprise i) a sequence having at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to seq.id.45; and ii) comprises a selection marker defined by seq.id.47 and an origin of replication defined by seq.id.46.

In some embodiments, the vectors described herein are used in combination with at least one transfection-enhancing agent, such as a transfection-enhancing agent selected from oligonucleotides, lipid complexes, polymersomes, polymeric complexes, dendrimers, inorganic nanoparticles, and cell-penetrating peptides.

The vectors described herein can be used for efficient transfer and/or amplification of the nucleic acid sequences of the invention in an amplification biotechnological production unit (example 3).

The amplification product in an amplification biotechnological production unit (e.g., a yeast cell) can be isolated and subsequently translated in another biotechnological production unit (e.g., a human cell).

Accordingly, the present invention is based, at least in part, on the following findings: the vectors described herein are capable of efficiently amplifying the nucleic acids described herein and efficiently producing combined virus-like proteins with limited replication capacity but high antigenicity. The nucleic acids of the invention are subjected to the above procedure to produce a dispersion comprising proteins and other building blocks.

Suitable separation methods known to the person skilled in the art, such as centrifugation or chromatography, can be used to separate these building blocks and, if desired, to purify them from the residues of the production cell lines or other production aids or organisms used.

In some embodiments, the building blocks described herein are purified using at least one separation method selected from chromatography, precipitation, ultracentrifugation, tangential flow filtration, and enzymatic digestion.

These optionally purified viral envelopes or fragments thereof represent the basis of a vaccine, which is then transferred to different dosage forms depending on the type of application.

Typically, adjuvants, stabilizers are used for this purpose to improve shelf life, salts and buffers. Thus, a vaccine is the product of a long-chain fully synthetic nucleic acid as described herein.

In some embodiments, the present invention relates to a viral envelope, a viral envelope fragment and/or a viral envelope protein obtainable by gene expression using at least one nucleic acid according to the invention, using a vector according to the invention, using a kit according to the invention or a biotechnological production unit according to the invention, wherein the viral envelope, viral envelope fragment and/or viral envelope protein packages at least one nucleic acid according to the invention.

In some embodiments, the invention relates to a viral envelope obtainable by gene expression using at least one nucleic acid according to the invention, using a vector according to the invention, using a kit according to the invention or a biotechnological production unit according to the invention.

As used herein, the term "viral envelope" refers to a protein assembly, such as a protein layer having a stabilizing function on a nucleotide sequence (e.g., a nucleotide sequence of the present invention). In some embodiments, the viral envelopes described herein enable assimilation of the nucleotide sequences of the invention into human cells. In some embodiments, the viral envelope described herein comprises a spike protein, an envelope protein, and a membrane protein.

In some embodiments, the invention relates to a viral envelope fragment obtainable by gene expression using at least one nucleic acid according to the invention, using a vector according to the invention, using a kit according to the invention or a biotechnological production unit according to the invention.

As used herein, the term "viral envelope fragment" refers to at least two assembled proteins that form an incomplete viral envelope.

In some embodiments, the invention relates to a viral envelope protein obtainable by gene expression using at least one nucleic acid according to the invention, using a vector according to the invention, using a kit according to the invention or a biotechnological production unit according to the invention.

As used herein, the term "viral envelope protein" refers to at least one protein that can form part of the viral envelope.

As used herein, the term "packaged" means at least partially enclosed and/or connected. In some embodiments, the packaging nucleotide of the invention in the viral envelope, viral envelope fragment, and/or viral envelope protein is capable of entering a human cell.

The products of the nucleic acids and/or vectors of the invention show particularly high antigenic similarity to the corresponding functional virus if the products are contained in the viral envelope, fragments of the viral envelope and/or proteins of the viral envelope. Thus, the elicited/induced immune response will likely induce an immune response that is particularly beneficial to actual contact with a functional virus.

Nucleotides packaged in the viral envelope, viral envelope fragment, and/or viral envelope protein can be transferred into a human cell of a subject and the production of the viral protein in the human cell is induced. This results in prolonged and enhanced exposure of antigenic virus-like proteins with limited replication capacity.

Accordingly, the present invention is based, at least in part, on the following findings: the vectors described herein are effective in producing combinatorial virus-like proteins with limited replication capacity but similar antigenic effect as the original virus.

In some embodiments, the invention relates to a vector of the invention for use in therapy.

In some embodiments, the invention relates to a biotechnological production unit of the invention for use in therapy.

In some embodiments, the invention relates to a viral envelope, viral envelope fragment and/or viral envelope protein of the invention for use in therapy.

As used herein, the term "treatment" (and grammatical variants thereof, such as "treat" or "treating") refers to a clinical intervention that attempts to alter the natural course of the individual to be treated, and may be performed for prophylaxis or during clinical pathology. Desirable therapeutic effects include, but are not limited to, prevention of occurrence or recurrence of disease, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, decreasing the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis.

In some embodiments, the invention relates to the vectors, biotech production units, viral envelopes, viral envelope fragments, and/or viral envelope proteins of the invention for use in the treatment of SARS-CoV-2 infection.

In some embodiments, the invention relates to a vector, biotech production unit, viral envelope fragment, and/or viral envelope protein of the invention for use in the prevention of SARS-CoV-2 infection.

In some embodiments, the invention relates to a vector, biotech production unit, viral envelope fragment and/or viral envelope protein of the invention for use in the treatment of active SARS-CoV-2 infection.

In some embodiments, the invention relates to a vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to the invention and a product obtainable by gene expression using at least one nucleic acid according to the invention in a production organism.

In some embodiments, the invention relates to a vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to the invention, and a product obtainable by using a vector according to the invention in a production organism.

In some embodiments, the invention relates to a vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to the invention, and a product obtainable by using a kit according to the invention in a production organism.

In some embodiments, the invention relates to a vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to the invention, and a product obtainable by gene expression using a kit according to the invention, in particular comprising a viral envelope, a viral envelope fragment and/or a viral envelope protein according to the invention, using a vector according to the invention, by using at least one nucleic acid according to the invention in a production organism.

As used herein, the term "vaccine" refers to any agent or composition capable of inducing/eliciting an immune response in a host and allowing the treatment and/or prevention of infection and/or disease. Thus, non-limiting examples of such agents include proteins, polypeptides, proteins/polypeptide fragments, immunogens, antigens, peptide epitopes, mixtures of proteins, peptides or epitopes, and nucleic acids, genes and/or portions of genes (encoding the polypeptide or protein of interest or fragments thereof).

As used herein, the term "against (against) the coronavirus SARS-CoV-2" refers to the treatment and/or prevention of SARS-CoV-2 infection.

Structural proteins of coronaviruses have been shown to elicit immune responses (see, e.g., li, J.Y., et al, 2020, virus research,286, 198074, walls, A.C., et al, 2020, cell,181 (2), 281-292.e.6, chen, Z, et al, 2004, clinical chemistry,50 (6), 988-995 Peng, Y, et al, 2020, nature immunology,21 (11), 1336-1345). The provided tools and methods are capable of inducing/eliciting equivalent immune responses by generating and administering vaccines with equivalent epitopes and/or particles with reduced immune escape mechanisms. In some embodiments, the vaccine induces the production of particles having limited replication capacity in the subject.

These vaccines are therefore quite different from classical vaccines, which are usually derived from animal sera and therefore they are not molecularly uniform. Production from animal organisms is traditionally the method of choice. However, molecularly unclear products lead to a number of quality problems and variation between production batches. This is also associated with long approval periods and side effects that are usually only found at a later stage. Thus, molecularly defined product compositions that can be obtained using nucleic acids according to the invention are advantageous.

In addition, the vaccines described herein are well defined and provide a broad range of antigenic epitopes. This leads to the advantage that the vaccine has a low or undesirable need for adjuvants to enhance the immune response. Such adjuvants that enhance the immune response are often associated with side effects (such as allergic reactions) in some patients. Furthermore, the main active component of the vaccine as described herein is protein-based and therefore more thermostable than other vaccines (e.g. RNA vaccines). Thus, the vaccine of the present invention is easy to transport and store due to its stability.

Accordingly, the present invention is based, at least in part, on the following findings: the vaccines as described herein are particularly suitable for use against coronavirus SARS-CoV-2.

In some embodiments, the invention relates to a kit comprising two or more nucleic acids according to the invention.

In some embodiments, the present invention relates to a kit comprising at least two nucleic acids selected from the group consisting of seq.id.35, seq.id.36, seq.id.37 and seq.id.38.

In this combination of vectors, the kit is capable of producing human viral proteins.

In addition to the nucleic acids, the invention also relates to a kit comprising two or more nucleic acids, wherein the nucleic acids are deoxyribonucleic acids (DNA) according to one of the preceding claims and/or corresponding ribonucleic acids (RNA) having a corresponding base pair sequence. In other words, the corresponding ribonucleic acid has a sequence portion as defined above, wherein thymine (T) is replaced by uracil (U).

The kits described herein can be prepared by collecting the necessary biotechnological production units and reagents. If the nucleic acids contained in the kit are present in the form of DNA, it is further preferred that they are present in at least one plasmid, preferably in two or more plasmids. This allows for an easy introduction of the nucleic acids into the respective biotechnological production unit, as described below in the context of the specific examples.

In a specific embodiment of the invention, the kit of the invention (to be prepared in the context) or the method and use of the invention may further comprise or be provided with an instruction manual. For example, the instruction manual may instruct the skilled person (how) to use the kit of the invention in the diagnostic uses provided herein and according to the invention. In particular, the instruction manual may comprise instructions for using or applying the methods or uses provided herein.

Accordingly, the present invention is based, at least in part, on the following findings: which is capable of efficiently and safely producing viral particles and/or parts thereof.

According to a further aspect, the present invention therefore also relates to a biotechnological production unit comprising at least one plasmid as defined above, in particular two or more plasmids. The production unit on which this further aspect of the invention is based is generally a production organism or cell line known to the skilled person for said purpose.

According to a further aspect, the invention also relates to products obtained by using the corresponding long-chain fully synthetic nucleic acids in suitable production organisms or cell lines. These products belong to the class of envelope proteins, usually with additional sugar or fatty acid groups. In particular, this further aspect thus relates to a viral envelope, a fragment of a viral envelope and/or a viral envelope protein obtainable by gene expression using a nucleic acid or using a kit as defined above.

What is important here is that the allocation is mathematically unambiguous: nucleic acid i produces product i which depends precisely on it. Even a slightly different nucleic acid j production is precisely dependent on its other product j. Two relationships between the product and the nucleic acid are unambiguous and describable. Each type of product k can be assigned to a nucleic acid k. Thus, it is reasonable to state a direct relationship between the nucleic acid and the product (i.e., the viral envelope or fragment thereof).

Wherever alternatives to the various separable features are presented herein as "embodiments," it should be understood that these alternatives can be freely combined to form the discrete embodiments of the invention disclosed herein.

It should be mentioned that the assembly of the viral envelope proceeds at different rates and with different purity depending on the organism and type, so that in practice the envelope and its fragments are always found together. However, if desired, they may be isolated by conventional methods.

In some embodiments, the envelopes described herein are purified using at least one purification method selected from chromatography, precipitation, ultracentrifugation, tangential flow filtration, and enzymatic digestion.

According to a further aspect of the invention, the direct product of the long-chain nucleic acid of the invention is thus converted into a vaccine by optional purification steps and possible auxiliary means. In particular, this further aspect therefore relates to a vaccine comprising a product obtainable by gene expression using at least one nucleic acid or a kit as defined above in a production organism, in particular comprising one or more of the above-mentioned protein components or parts thereof.

The vaccine is typically a physiological saline solution containing the above-mentioned additives and usually a small concentration of the above-mentioned viral envelope and/or fragment.

Although the vaccines described herein are less dependent on the effect of an adjuvant than other vaccines, the vaccines may still include an adjuvant to enhance the effect of the vaccine. In some embodiments, the vaccine comprises at least one adjuvant selected from the group consisting of inorganic compounds (e.g., potassium alum, aluminum hydroxide, aluminum phosphate, calcium hydrogen phosphate), oils (e.g., paraffin oil, peanut oil), bacterial products, saponins, cytokines (e.g., IL-1, IL-2, IL-12), and squalene.

In some embodiments, the vaccine is administered by at least one route of administration selected from oral administration, rectal administration, intrarectal administration, inhalation, nasal administration, parenteral administration, intramuscular administration, subcutaneous administration, and intradermal administration.

Depending on the dosage form, a typical vaccine is injected or may be administered mucosally.

As mentioned above, the vaccine is particularly a vaccine against the coronavirus SARS-CoV-2. In particular, it comprises at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1, b2, c1 or c2, d1 or d2, where

(i) Protein component a comprises the sequence of the S protein similar to SARS-CoV-2 defined by seq.id.14 and seq.id.18; and

(ii) Protein component b1 comprises the sequence of the envelope protein E analogous to SARS-CoV-2 shown in SEQ.ID.6 and SEQ.ID.21 and protein component b2 comprises the sequence of the envelope protein E analogous to MHV59A or equivalent proteins according to SEQ.ID.8; and

(iii) Protein component c1 comprises the sequence of the membrane protein M analogous to SARS-CoV-2 according to SEQ.ID.10 and SEQ.ID.22 and protein component (c 2) comprises the sequence of the membrane protein M analogous to MHV59A or equivalent protein according to SEQ.ID.12; and

(iv) Protein component d1 comprises the sequence of nucleocapsid phosphoprotein N analogous to SARS-CoV-2 according to seq.id.2 and seq.id.26 and protein component d2 comprises the sequence of nucleocapsid phosphoprotein N analogous to MHV59A of seq.id.4 or an equivalent protein.

It should be noted that protein components a, b1, b2, c1, c2, d1 or d2 are similar but not identical to the corresponding naturally occurring analogs in that they are produced from synthetic nucleic acids having a sequence that differs from the sequence of the corresponding natural nucleic acids.

Protein component a disclosed in the present specification comprises a sequence according to seq.id.14 and seq.id.18. In some embodiments, protein component a comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.14.

In some embodiments, protein component a comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.18.

Protein component b1 disclosed in the present specification comprises a sequence according to seq.id.6 and seq.id.21. In some embodiments, protein component b1 comprises a sequence having at least 90%, having at least 91%, having at least 92%, having at least 93%, having at least 94%, having at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.6.

In some embodiments, protein component b1 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.21.

Protein component b2 disclosed in the present specification comprises a sequence according to seq.id.8. In some embodiments, protein component b2 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.8.

Protein component c1 disclosed in the present specification comprises a sequence according to seq.id.10 and seq.id.22. In some embodiments, protein component c1 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.10.

In some embodiments, protein component c1 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.22.

Protein component c2 disclosed in the present specification comprises a sequence according to seq.id.12. In some embodiments, protein component c2 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.12.

Protein component d1 disclosed in the present specification comprises a sequence according to seq.id.2 and seq.id.26. In some embodiments, protein component d1 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.2.

In some embodiments, protein component d1 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.26.

Protein component d2 disclosed in the present specification comprises a sequence according to seq.id.4. In some embodiments, protein component d2 comprises a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.4.

A protein component having a certain% sequence identity to an amino acid sequence described herein may be obtained, for example, by insertion, deletion, substitution and/or modification of at least one amino acid, but not more than 10%, not more than 9%, not more than 8%, not more than 7%, not more than 6%, not more than 5%, not more than 4%, not more than 3%, not more than 2%, not more than 1%, not more than 0.9%, not more than 0.8%, not more than 0.7%, not more than 0.6%, not more than 0.5%, not more than 0.4%, not more than 0.3%, not more than 0.2% or not more than 0.1% of the amino acid sequence relative to the amino acid sequence of seq.id.2, seq.id.4, seq.6, seq.id.8, seq.id.10, seq.id.12, seq.14, seq.id.18, seq.id.21, seq id.22 and/or seq id.26. Such insertions, deletions, substitutions, and/or modifications can be made based on the corresponding nucleotide sequence described herein (e.g., a nucleotide sequence encoding a SARS-CoV-2 variant of a mutant variant of a protein component described herein) that encodes the desired insertion, deletion, substitution, and/or modification.

Insertions, deletions, substitutions and/or modifications may also be the result of post-translational modifications. In some embodiments, the protein components described herein are post-translationally modified to improve the production process. In some embodiments, the protein component described herein is post-translationally modified to improve at least one protein property of the protein component, such as a protein property selected from the group consisting of antigenicity, protein stability, pharmacokinetics, pharmacodynamics, interaction with a drug, and interaction with an adjuvant. In some embodiments, the protein component described herein is post-translationally modified by a technique selected from at least: addition of functional groups, linkage to other proteins or peptides, chemical modification of amino acids (e.g. citrullination, deamination, deamidation, post-translational elimination), disulfide bridges, cysteine amino acid linkages, peptide bond cleavage, isoaspartic acid formation, racemization and protein splicing.

Thus, an amino acid sequence described herein does not necessarily have a proportional% sequence identity overlap with a nucleotide sequence described herein. In some embodiments, the amino acid sequence of the invention differs from the sequence described in seq.id.2, seq.id.4, seq.id.6, seq.id.8, seq.id.10, seq.id.12, seq.id.14, seq.id.18, seq.id.21, seq.id.22 and/or seq.id.26 by at least 10%, at least 9%, at least 8%, at least 7%, at least 6%, at least 5%, at least 4%, at least 3%, at least 2%, at least 1%, at least 0.9%, at least 0.8%, at least 0.7%, at least 0.6%, at least 0.5%, at least 0.4%, at least 0.3%, at least 0.2%, at least 0.1% more than the altered nucleotide amino acid sequence (which differs from the nucleotide sequence described herein).

In some embodiments, the invention relates to a vaccine according to the invention comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1, b2, c1 or c2, d1 or d2, wherein

(i) Protein component a comprises

a) A sequence according to seq id.14 similar to the S protein of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.14; or

b) A sequence according to seq id.18 similar to the S protein of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.18;

(ii) Protein component b1 comprises

a) A sequence according to seq id.6 that is similar to envelope protein E of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.6; or

b) A sequence according to seq id.21 similar to envelope protein E of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.21; and

protein component b2 comprises a sequence according to seq id.8 similar to the envelope protein E of MHV59A or an equivalent protein comprising a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq id.8;

(iii) Protein component c1 comprises

a) A sequence according to seq id.10 that is similar to envelope protein E of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq id.10; or

b) A sequence according to seq.id.22 similar to membrane protein M of SARS-CoV-2, or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% sequence identity to seq.id.22; and

protein component c2 comprises a sequence according to seq id.12 similar to the membrane protein M of MHV59A or an equivalent protein comprising a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq id.12; and

(iv) Protein component d1 comprises

a) A sequence according to seq id.2 similar to nucleocapsid phosphoprotein N of SARS-CoV-2 or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq id.2; or

b) A sequence according to seq.id.26 or a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq.id.no.26 similar to the nucleocapsid phosphoprotein N of SARS-CoV-2; and

protein component d2 comprises a sequence according to seq id.4 similar to the nucleocapsid phosphoprotein N of MHV59A or an equivalent protein comprising a sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% sequence identity to seq id.4.

In some embodiments, the present invention relates to a vaccine according to the present invention comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1, c1 and d1.

In some embodiments, the present invention relates to a vaccine according to the present invention comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components b1, c1 and d1.

In some embodiments, the present invention relates to a vaccine according to the present invention comprising at least two molecularly precisely defined protein components selected from protein components a, c1 and d1.

In some embodiments, the present invention relates to a vaccine according to the present invention comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1 and d1.

In some embodiments, the present invention relates to a vaccine according to the present invention comprising at least two molecularly precisely defined protein components selected from the group consisting of protein components a, b1 and c1.

In some embodiments, the invention relates to a vaccine according to the invention comprising at least two molecularly precisely defined protein components a and c1.

In some embodiments, the invention relates to a vaccine according to the invention comprising at least two molecularly precisely defined protein components a and d1.

In some embodiments, the invention relates to a vaccine according to the invention comprising at least two molecularly precisely defined protein components c1 and d1.

In some embodiments, the invention relates to a vaccine according to the invention comprising at least two molecularly precisely defined protein components a, and b1, c1, and d1.

In some embodiments, the invention relates to a vaccine according to the invention comprising at least three molecularly precisely defined protein components selected from the group consisting of protein components a, b1, c1 and d1.

In some embodiments, the invention relates to a vaccine according to the invention comprising three molecularly precisely defined protein components selected from the group consisting of protein components a, b1, c1 and d1.

Vaccines according to the invention comprising the protein components described herein can elicit a substantial and broad immune response. At the same time, the replication capacity of the vaccine may be limited because it does not replicate in the host body. Such limited replication capability may be achieved, for example, by omitting or changing the sequences required for efficient replication.

Accordingly, the present invention is based, at least in part, on the following findings: vaccines comprising combinations of protein components described herein may exhibit desirable replication capacity limitations while largely retaining antigenic potential.

Furthermore, the present invention relates to a method for producing a vaccine comprising the following successive steps: introducing at least one nucleic acid according to any of claims 1 to 10 into a biotechnological production unit, in particular a cell line, by transfection, starting from a nucleic acid-based mRNA, preparing by translation at least two protein components selected from the protein components a, b1, b2, c1, c2, d1 or d2, and purifying the protein components obtained thereof.

In some embodiments, the present invention relates to a method for producing a vaccine according to the present invention comprising the following successive steps:

a) The vector according to one of embodiments 10 to 14 is introduced into a biotechnological production unit, in particular a cell line,

c) Purifying the obtained protein fraction to obtain the vaccine according to the invention.

In some embodiments, the invention relates to a biotechnological production unit comprising at least one vector according to the invention.

The terms "biotechnological production unit" and "production organism" are used interchangeably herein and refer to at least one host cell, including progeny of such cells, organisms, and biotechnological units comprising such cells and/or progeny of such cells, into which a nucleic acid of the invention has been introduced for expression. Host cells include "transformants" and "transformed cells," which include the primary transformed cell and progeny derived therefrom, regardless of the number of passages. Progeny may not be identical in nucleic acid content to the parent cell, but may contain mutations. Included herein are mutant progeny that have the same function or biological activity as screened or selected in the originally transformed cell.

The term "amplification biotechnological production unit" refers to any biotechnological production unit that allows for an amplification of a large vector (e.g., more than 4000 bases, more than 10000 bases, more than 35000 bases). In some embodiments, the amplification biotech production unit described herein comprises a yeast cell.

In certain embodiments, the host cell is a stem cell. In other embodiments, the host cell is a differentiated cell.

The biotechnological production unit described herein is particularly useful if it comprises cells which allow the SARS-CoV-2 virus to enter, such that the cell product of the biotechnological production unit can enter cells of further biotechnological production units. This subsequent infection of biotechnologically produced cells facilitates and accelerates the process of bringing the vector into the host cell.

In some embodiments, the biotech production units described herein comprise cells that allow SARS-CoV-2 virus entry. In some embodiments, the biotech production units described herein comprise cells that express a human ACE2 receptor or a functional human-like ACE2 receptor. The human-like ACE2 receptor that allows entry of the SARS-CoV-2 virus is known to those skilled in the art (see, e.g., damas, J. Et al, 2020, proceedings of the National Academy of sciences,117 (36), 22311-22322).

In some embodiments, the biotech production units described herein comprise at least one cell type selected from HEK293, MDCK, chinese Hamster Ovary (CHO), SF9, vero, MRC 5, per.c6, PMK, and WI-38.

In some embodiments, the biotech production units described herein comprise cells that are at least partially human or cells that are at least partially human cell lines.

In some embodiments, the biotech production units described herein comprise cells of viral particles that allow for the production of selectively replicating nucleotides of the invention or vectors of the invention that are fully replicable in the cells of the biotech production units, but not or not significantly fully replicable in the cells of the human body. Such selective replication is achieved by cells comprising complementary proteins for replication of the viral particles (see e.g. the examples).

In some embodiments, the biotech production units described herein comprise cells that can express at least one protein for viral replication. In some embodiments, the biotech production units described herein comprise cells that can express at least one protein component for viral replication that is not encoded in a nucleotide sequence of the invention or a vector of the invention.

Host cell transduction by the vectors of the invention can be achieved by stable or transient transduction (see, e.g., stepanenko, a.a. and Heng, h.h.,2017, mutation Research/Reviews in Mutation Research,773, 91-103).

If the DNA is introduced into the production unit according to the first embodiment, this is generally done using a plasmid suitable for the purpose.

Alternatively, the DNA may be introduced into the biotechnological production unit by any kind of vector.

On the other hand, if RNA is introduced according to the second embodiment, a sequence encoding an RNA-dependent RNA polymerase (according to seq. Id.30) is introduced in addition to the sequence encoding the protein component a, b1, b2, c1, c2, d1 or d 2. This sequence makes it possible to first form a negative RNA strand from the positive RNA strand present as template and then to generate the corresponding messenger RNA therefrom.

In the context of this second embodiment of the procedure, the vaccine preferably obtained also comprises a fully synthetic long-chain ribonucleic acid (according to seq. Id.33 or 34), which can be obtained by enzymatic transcription.

Within the context of this second embodiment of the procedure, it is also preferred that the vaccine obtained further comprises a fully synthetic long-chain ribonucleic acid (according to seq.id.33 or 34), which is obtainable by T7 transcription of the sequence according to seq.id.28.

"a," "an," and "the" are used herein to refer to one or to more than one (i.e., to at least one, or to one or more) of the grammatical object of the article.

"or" is to be understood as meaning one, two or any combination thereof of the alternatives.

"and/or" should be understood to mean one or both of the alternatives.

Throughout this specification, unless the context requires otherwise, the words "comprise", "comprises" and "comprising" will be understood to imply the inclusion of a stated step or element or group of steps or elements but not the exclusion of any other step or element or group of steps or elements.

The terms "comprising" and "including" are used synonymously. "preferably" refers to one option in a series of options, without excluding others. "for example" refers to an example, not limited to the example mentioned. "consisting of 8230; \8230; composition" is meant to include and be limited to anything following the phrase "consisting of 8230; \8230; composition".

Reference throughout this specification to "one embodiment," "a particular embodiment," "a related embodiment," "an embodiment," "another embodiment," "some embodiments," "a specific embodiment," or "other embodiments," or combinations thereof, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the foregoing phrases appearing in various places throughout the specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. It should also be understood that a positive recitation of a feature in one embodiment (recitations) serves as a basis for excluding that feature from a particular embodiment.

The present invention is further explained by the following design examples in conjunction with the accompanying drawings, which do not limit the scope of the present invention described in the claims.

Drawings

FIG. 1: plasmid map of monocistronic expression plasmid encoding nucleocapsid protein (N) (SEQ. ID.35), envelope protein (E) (SEQ. ID.36), membrane protein (M) (SEQ. ID.37) and spike glycoprotein (S) (SEQ. ID.38) of SARS-CoV 2. The numbers inside the plasmid map represent the DNA coordinates in the base pair. The protein coding sequences for N, E, M and S are indicated by arrows and represent DNA and protein sequences having seq.id.1, 2, 3 and 4 (N), 5, 6, 7 and 8 (E), 9, 10, 11 and 12 (M), 13 and 14 (S) as shown in the sequence listing.

FIG. 2: genomic map of polycistronic expression construct COVAX191 Δ N (seq.id.33 and 39) (upper panel) which together with monocistronic expression plasmid pcDNA34 syn N (seq.id.35) (lower panel) can be used for vaccine production in the cell line as shown in example 2. Numbers refer to DNA coordinates in kilobases (K) for COVAX191 Δ N and to base pair positions of pcDNA34 Syn N construct (seq. Id.35). The protein coding sequences for the polyprotein 1a and 1b, E, MS (upper panel) and the nucleocapsid protein syn N (lower panel) are indicated by arrows.

FIG. 3: agarose gel electrophoresis size separation of plasmid-based monocistronic expression constructs of nucleocapsid protein (N), envelope protein (E), membrane protein (M) and spike glycoprotein (S). The MHV a59 (MHV) -derived constructs of nucleocapsid protein (N), envelope protein (E) and membrane protein (M) are shown on the left side of the gel. The derivative constructs based on the nucleocapsid protein (N), the envelope protein (E), the membrane protein (M) and the spike glycoprotein (S) of SARS-CoV2 are shown on the right side of the gel.

FIG. 4: schematic representation of the corresponding DNA sequencing overlay for the circular 40,556bp DNA construct COVAX191 Δ N (seq.id.40) (upper panel) and the 38,383bp DNA construct COVAX191 Δ N Δ HE (seq.id.40) (lower panel). Arrows indicate the positions of the protein coding sequences of the recoded CDS of

replicative polyprotein

1A and 1B (1a, 1b), hemagglutinin Esterase (HE), spike glycoprotein (S), envelope protein (E) and membrane protein (M). The whole genome of COVAX191 Δ N and COVAX191 Δ N Δ HE was assembled from 6 synthetic DNA blocks using a single lithium acetate yeast transformation and selection for the auxotrophic URA3 marker.

FIG. 5: schematic representation of SARS-CoV-2 genome and deletion variants produced.

Table S1: examples of DNA Assembly efficiency of COVAX191 in Saccharomyces cerevisiae (yeast).

Examples

The following examples illustrate how long-chain nucleic acids of the invention encoding envelope proteins E, M, N and S can be produced and used in biotechnological processes to stimulate cells to produce coronavirus envelopes or fragments thereof.

For production, the (digital) sequences according to the invention are transferred into the corresponding physically present long-chain total synthetic nucleic acid molecules by the process of chemical DNA synthesis.

Example 1

In a first example, the resulting long-chain fully synthetic nucleic acids encoding envelope proteins E, M, N and S are monocistronic, i.e. they are generated under the control of separate promoters (SV 40, CMV, EF-1, chicken beta actin promoter or hybrid promoter) and optionally other translation initiation signals (Kozak consensus sequence) and nuclear mRNA export signals (Chuck Wood sequence) into expression plasmids for eukaryotic cells. Sequences as shown in seq.id.35, seq.id.36, seq.id.37 and seq.id.38 and fig. 1 should be taken as examples of such expression systems. Other embodiments for other expression plasmids, corresponding resistance genes and promoters are possible and known to the person skilled in the art.

The 4 expression plasmids obtained were amplified in E.coli, purified by standard chemical-physical procedures and then introduced into eukaryotic cell lines (HEK 293, chinese Hamster Ovary (CHO), SF9, vero) by transfection. Transfection is performed by standard procedures, such as calcium phosphate, lipofection, electroporation.

After transfection, starting from the transfected plasmid DNA, the cell starts to translate messenger RNA (mRNA), thereby expressing envelope proteins E, M, N and S by translation. These proteins assemble spontaneously in cells to form the coronavirus envelope and are subsequently released by exocytosis from the cells into the medium, where they accumulate after 5-7 days.

Chemical-physical methods are used to purify envelope proteins, viral envelopes and fragments thereof. For this purpose, the cell culture supernatant is separated from the cells by centrifugation. In a subsequent step, the virus is further purified of impurities in the envelope and other components of the culture medium by chromatographic column separation methods. The material thus obtained in pure form (consisting of the coronavirus envelope) forms the basis of a vaccine, which is then converted into various administration forms depending on the type of application. Typically, adjuvants, stabilizers are used for this purpose to improve shelf life, salts and buffers. Thus, a vaccine is the product of a long chain fully synthetic nucleic acid as described herein.

Example 2

In a second embodiment, long chain total synthetic nucleic acids encoding envelope proteins E, M and S are expressed together with total synthetic nucleic acids encoding RNA-dependent RNA polymerases. In this polycistronic expression system, envelope proteins E, M and S are transcribed directly from the negative RNA strand, including RNA-dependent RNA polymerase, as shown in sequences seq.id.39 and seq.id.40, and as shown in fig. 2. If not all classes of envelope proteins of sequence groups A-D are RNA-dependent expressed, the full set of envelope proteins can be expressed using additional expression plasmids as described in example 1 for biotechnological production of viral envelopes in cell lines. In example 2, an expression plasmid encoding the N protein was used for this purpose (seq. Id.35) (see fig. 2).

The purification of plasmids, transfection of long-chain nucleic acids and purification of the viral envelope mainly follow the sequence of methods described in example 1. However, the method comprises an additional step wherein the long-chain nucleic acid, as described in seq.id.39 and seq.id.40, is converted by T7 RNA polymerase into the corresponding RNA form according to seq.id.33 and seq.id.34 before transfection. This positive RNA strand results in the production of an RNA-dependent RNA polymerase in the cell line, which produces a negative RNA strand therefrom. Transcription of messenger RNA (mRNA) from this negative RNA strand then occurs, which results in the production and assembly of envelope proteins in the viral envelope.

The vaccine produced in this way differs from the vaccine described in the first example 1 in that, in addition to the envelope protein obtained by gene expression of the corresponding deoxyribonucleic acid, it contains a fully synthetic long-chain ribonucleic acid, which is expressed by the T7 transcription of the sequences seq.id.39 and seq.id.40.

The second example 2 has the advantage over the first application example that it produces a viral envelope that propagates itself in helper cell lines expressing the N protein. This is possible because the viral envelope formed in this way additionally contains positive RNA strands which code for an RNA-dependent RNA polymerase and envelope proteins E, M and S. If these viral envelopes are taken up by the cell, the cell itself is stimulated to produce the viral envelope. If the cell expresses the N protein in free form, as is the case with vaccine producing cell lines, a self-replicating viral envelope is formed. This simplifies the production process and can be performed without expensive transfection reagents. If the target cells do not express any N protein, the viral envelope is also formed from it, but they have no packaged RNA strand and are no longer able to self-replicate. These viral envelopes have the same chemical/physical structure and the same antigenicity as those produced by the production method shown in example 1. Example 2 allows the production of viral envelopes, fragments and viral envelope proteins in other helper cell lines and production organisms, as well as direct application as RNA vaccines.

Method：

Culture of bacterial and yeast strains

Coli (E.coli) DH 5. Alpha. Was cultured in Luria-Broth (LB) at 37 ℃. Saccharomyces cerevisiae VL6-48N (Kouprina et al 2006 Methods in mol. Biol.349, 85-101) was cultured in yeast peptone-dextrose (YPD) medium or uracil-free synthetic deficient (dropout) (SD) medium at 30 ℃.

Sequence design and de novo DNA synthesis.

The DNA sequences of the monocistronic and polycistronic expression constructs were assembled from the sequence portions disclosed in the attached sequence listing (seq. Id.1 to 40). Synthetic constraints were removed computationally by synonymous codon substitutions and the application of the desired base substitutions within the intergenic sequence. To define the optimal reverse synthetic assembly pathway, the synthetically optimized DNA design is hierarchically divided into smaller DNA fragments suitable for low-cost synthesis by commercial vendors. The partitioning strategy is designed as a four-step hierarchical assembly process. Subblocks of size 1.4kb (kilobases) were assembled into 5.4kb blocks and further assembled into fragments of size 16kb, which were then assembled into final COVAX constructs of 35 to 40 kb. The linear DNA assembly portion has homologously overlapping and nested 3 'prefix and 5' suffix sequences at the ends to integrate the assembled DNA portion into the vector and allow for hierarchical assembly of the final COVAX DNA design. The DNA assembly part was obtained from commercial suppliers as a sequence-verified cloned plasmid construct and double-stranded linear DNA by low-cost DNA synthesis.

Production of monocistronic expression constructs:

synthetic nucleic acids encompassing the complete protein coding sequence of the S protein of SARS-2 CoV, the M protein, the N protein and the E protein of SARS-CoV-2 or MHV are amplified from sequence-verified synthetic DNA by polymerase amplification technology (PCR). The translation initiation site preceding the initiation codon is introduced by means of oligonucleotide primers. The PCR products were separated by agarose Gel electrophoresis according to their molecular weight and then purified on a nucleic acid probe (Nucleospin) column (Nucleospin Gel and PCR Clean Up Kit, macherey Nail). The PCR product was cloned into pcDNA3.4 vector using Topo-TA cloning kit (TOPO-TA cloning kit, thermoFisher). The molecular weight of the plasmids was determined by agarose gel electrophoresis (fig. 3) and the DNA sequence was checked by Sanger sequencing.

Production of polycistronic COVAX DNA constructs:

the DNA assembly part of the polycistronic COVAX DNA construct was released from the plasmid by restriction digestion using type IIS restriction enzymes (BbsI, bspQI, pacI and PmeI (New England Biolabs)). Equimolar amounts of the DNA insert (100ng, 0.115pmol) and the linearized vector pXMCS2 (100ng, 0.038pmol) were incubated with T5 exonuclease, phusion polymerase and Taq DNA ligase at 50 ℃ for 1 hour. After isothermal assembly, the constructs were electroporated into e.coli DFI5 α cells (BioRad MiniPulser). Cells were incubated in LB medium for 1 hour and plated on LB plates. Fragments and complete COVAX constructs were assembled from yeast by yeast recombination according to the lithium acetate transformation method using plasmid pMR10Y (pMR 10:: CEN/ARS:: URA3, christen et al 2015 ACS Synthetic biology,4, 927-934) (Gietz et al 2007, nature protocols,2, 31-34). Saccharomyces cerevisiae VL6-48N was grown overnight in 5ml YPD, diluted 1. Cells were harvested by centrifugation at 1,000rcf for 5min, washed with 25ml distilled water and centrifuged at 3,000rcf for 5min. The precipitate was dissolved in 1ml of a lithium acetate mixture (0.1M lithium acetate, 0.01M Tris-HCl, pH 7.5,0.001M EDTA, pH 8.0). Next, 100. Mu.l of single-stranded salmon sperm DNA (1% w/v salmon sperm DNA (ssDNA), 0.01M Tris-HCl, pH 7.5,0.001M EDTA, pH 8.0) and 6ml of PEG-mixture (40% w/v poly (ethylene glycol) 3015-3685g/mol,0.01M Tris-HCl, pH 7.5,0.001M EDTA, pH 8.0) were added. From the PEG cell mixture, a 710 μ l aliquot was combined with 100ng of the digested DNA block and 250ng of linearized pMR10Y vector (Pacl, pmel). The samples were incubated at 30 ℃ for 30 minutes. After incubation, 70 μ l of dimethyl sulfoxide (DMSO) was added and the sample was heat shocked at 42 ℃ for 15 minutes. Cells were harvested by centrifugation at 1000rcf for 2 minutes, then plated on SD plates without uracil and incubated at 30 ℃ for 3 days until colonies became visible (see table S1).

Sequence verification of COVAX DNA constructs.

Sequence validation of the assembled DNA constructs was performed on an iSeq instrument (Illumina) using Nextera DNA Flex library preparation kit. Genomic DNA of ura + yeast transformants was fragmented and processed according to the labeling protocol specified by the manufacturer. Sequences were calculated de novo from the read sequences and the created contigs (contigs) were compared to the reference sequence using CLC Genomics Workbench software (Quiagen). Complete assembly of COVAX191 Δ N and COVAX191 Δ HEN was confirmed with a fully closed sequence overlay (fig. 4).

Example 3

Yeast clones, each containing a circular sequence (viral sequence, T7 promoter and polyA signal and vector, all in one yeast artificial chromosome or "YAC"), were grown, harvested and their YACs extracted. The YACs thus obtained were cleaved with the restriction enzyme EagI, resulting in double stranded DNA molecules that were directly linearized after the polyA signal. After making these DNA molecules rnase-free by standard treatment with proteinase K, followed by Trizol (phenol/chloroform) extraction, single-stranded RNA corresponding to the genome of the vaccine virus was obtained by in vitro transcription using T7 polymerase. The RNA thus obtained is transfected into suitable cell lines (HEK 293T or Vero cells). In the case of the positive control, HEK293 or Vero cells, unaltered by the full length construct "GBsyn _ V33", support replication of the RNA genome, production of subgenomic mrnas and thus translation into viral proteins. These viral proteins form, together with the positive-stranded RNA genome and components from the cell membrane, a progeny virus, in this case the wild-type, native SARS-CoV-2 virus. In the case of deletion mutants, one or more genes deleted in the viral genome are transfected into the cell line in DNA form, resulting in transient expression of one or more proteins, thereby providing the deletion factor required to enable the production of progeny virus. Alternatively (and preferably), culturing those cells under selective pressure results in stable integration of one or more genes into the cell genome from which one or more proteins are expressed continuously (for expression we understand that mRNA is produced from a gene and subsequently translated into a protein). Such cells, which transiently or stably express proteins made from genes deleted in the vaccine virus genome, are capable of continuous production of vaccine viruses, which are characterized by a full set of structural proteins and a vaccine virus genome with one or several gene deletions. The vaccine virus thus obtained is purified in a so-called downstream processing (DSP) process characterized by clarification (separation of cells from the vaccine virus), DNA digestion with a totipotent nuclease (Benzoase), ultrafiltration/diafiltration ("UF/DF") and finally sterile filtration (0.22 μm filtration).

Sequence listing

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<120> Total synthetic Long-chain nucleic acids for vaccine production to defend coronavirus

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atggtgtctg ataatggacc tcaaaatcag cgaaatgcac ctcgcattac gtttggtgga 60

ccatcagatt caactggcag taaccagaat ggagaacgaa gtggtgcgcg atcaaaacaa 120

cgccgcccgc aaggtttacc caataatact gcgtcttggt tcaccgctct cactcaacat 180

ggcaaggaag atttaaaatt ccctcgagga caaggcgttc caattaacac caatagcagt 240

ccagatgacc aaattggcta ctaccgccgc gccacaagac gaattcgtgg tggtgatggt 300

aaaatgaaag atctcagtcc aagatggtat ttctactatc taggaactgg gccagaagct 360

ggacttcctt atggtgctaa caaagatggc atcatatggg ttgcaactga gggagccttg 420

aatacaccaa aagatcacat tggcaccaga aatcctgcta acaatgctgc aatcgtgcta 480

caacttcctc aaggaacaac attaccaaaa ggtttttacg cagaagggtc tagaggtgga 540

agtcaagcct cttctagatc atcatcacgt agtcgcaaca gttcaagaaa ttcaactcca 600

ggttcaagta gaggaacttc tcctgctaga atggctggaa atggaggtga tgctgctctt 660

gctttgttac tacttgacag attgaaccag cttgagagca aaatgtctgg taaaggccaa 720

caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa gaagcctaga 780

caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag acgtggtcca 840

gaacaaactc aaggaaattt tggggatcag gaactaatca gacaaggaac tgattacaaa 900

cattggccgc aaattgcaca atttgctcct tctgcttcag cgttctttgg aatgtcgaga 960

attggaatgg aagtcacacc ttcgggaaca tggttgacct atacaggtgc catcaaattg 1020

gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca tattgacgca 1080

tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc tgatgaaact 1140

caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc tgctgcagat 1200

ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc aactcaggcc 1260

taa 1263

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<223> synthetic _ nucleocapsid _ protein _ Sars-CoV2

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Met Val Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile

1 5 10 15

Thr Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu

20 25 30

Arg Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn

35 40 45

Asn Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp

50 55 60

Leu Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser

65 70 75 80

Pro Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg

85 90 95

Gly Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr

100 105 110

Tyr Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys

115 120 125

Asp Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys

130 135 140

Asp His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu

145 150 155 160

Gln Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly

165 170 175

Ser Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg

180 185 190

Asn Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro

195 200 205

Ala Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu

210 215 220

Leu Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln

225 230 235 240

Gln Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser

245 250 255

Lys Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr

260 265 270

Gln Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly

275 280 285

Asp Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln

290 295 300

Ile Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg

305 310 315 320

Ile Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly

325 330 335

Ala Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile

340 345 350

Leu Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu

355 360 365

Pro Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro

370 375 380

Gln Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp

385 390 395 400

Leu Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp

405 410 415

Ser Thr Gln Ala

420

<210> 3

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<212> DNA

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<220>

<223> COVAX191_N

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gctggtaatg gaatcctcaa gaaaaccact tgggctgacc aaaccgagcg tggaccaaat 120

aatcaaaata gaggcagaag gaatcagcca aagcagactg caactactca acccaactcc 180

gggagtgtgg ttccccatta ctcctggttt tctggcatta cccagttcca aaagggaaag 240

gagtttcagt ttgcagaagg acaaggagtg cctattgcca atggaatccc cgcttcagag 300

caaaagggat attggtatag acacaaccgc cgttctttta aaacacctga tgggcagcag 360

aagcaattac tgcccagatg gtatttttac tatcttggca cagggcccca tgctggagcc 420

agttatggag acagcattga aggcgtcttt tgggttgcaa acagccaagc ggacaccaat 480

acccgctctg atattgtcga aagggaccca agcagtcatg aggctattcc tactaggttt 540

gcgcccggca cggtattgcc tcagggcttt tatgttgaag gctctggaag gtctgccccg 600

gccagccgat ctggttcgcg gtcacaatcc cgtgggccaa ataatcgcgc tagaagcagt 660

tccaaccagc gccagcctgc ctctactgta aaacctgata tggccgaaga aattgctgct 720

cttgttttgg ctaagctcgg taaagatgcc ggccagccca agcaagtaac gaagcaaagt 780

gccaaagaag tcaggcagaa aattttaaac aagcctcgcc aaaagaggac tccaaacaag 840

cagtgcccag tgcagcagtg ttttggaaag agaggcccca atcagaattt tggaggctct 900

gaaatgttaa aacttggaac tagtgatcca cagttcccca ttcttgcaga gttggctcca 960

acagttggtg ccttcttctt tggatctaaa ttagaattgg tcaaaaagaa ttctggtggt 1020

gctgatgaac ccaccaaaga tgtgtatgag ctgcaatatt caggtgcagt tagatttgat 1080

agtactctac ctggttttga gactatcatg aaagtgttga atgagaattt gaatgcctac 1140

cagaaggatg gtggtgcaga tgtggtgagc ccaaagcccc aaagaaaagg gcgtagacag 1200

gctcaggaaa agaaagatga agtagataat gtaagcgttg caaagcccaa aagctctgtg 1260

cagcgaaatg taagtagaga attaacccca gaggatagaa gtctgttggc tcagatcctt 1320

gatgatggcg tagtgccaga tgggttagaa gatgactcta atgtgtaa 1368

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Met Val Ser Phe Val Pro Gly Gln Glu Asn Ala Gly Gly Arg Ser Ser

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Ser Val Asn Arg Ala Gly Asn Gly Ile Leu Lys Lys Thr Thr Trp Ala

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Asp Gln Thr Glu Arg Gly Pro Asn Asn Gln Asn Arg Gly Arg Arg Asn

35 40 45

Gln Pro Lys Gln Thr Ala Thr Thr Gln Pro Asn Ser Gly Ser Val Val

50 55 60

Pro His Tyr Ser Trp Phe Ser Gly Ile Thr Gln Phe Gln Lys Gly Lys

65 70 75 80

Glu Phe Gln Phe Ala Glu Gly Gln Gly Val Pro Ile Ala Asn Gly Ile

85 90 95

Pro Ala Ser Glu Gln Lys Gly Tyr Trp Tyr Arg His Asn Arg Arg Ser

100 105 110

Phe Lys Thr Pro Asp Gly Gln Gln Lys Gln Leu Leu Pro Arg Trp Tyr

115 120 125

Phe Tyr Tyr Leu Gly Thr Gly Pro His Ala Gly Ala Ser Tyr Gly Asp

130 135 140

Ser Ile Glu Gly Val Phe Trp Val Ala Asn Ser Gln Ala Asp Thr Asn

145 150 155 160

Thr Arg Ser Asp Ile Val Glu Arg Asp Pro Ser Ser His Glu Ala Ile

165 170 175

Pro Thr Arg Phe Ala Pro Gly Thr Val Leu Pro Gln Gly Phe Tyr Val

180 185 190

Glu Gly Ser Gly Arg Ser Ala Pro Ala Ser Arg Ser Gly Ser Arg Ser

195 200 205

Gln Ser Arg Gly Pro Asn Asn Arg Ala Arg Ser Ser Ser Asn Gln Arg

210 215 220

Gln Pro Ala Ser Thr Val Lys Pro Asp Met Ala Glu Glu Ile Ala Ala

225 230 235 240

Leu Val Leu Ala Lys Leu Gly Lys Asp Ala Gly Gln Pro Lys Gln Val

245 250 255

Thr Lys Gln Ser Ala Lys Glu Val Arg Gln Lys Ile Leu Asn Lys Pro

260 265 270

Arg Gln Lys Arg Thr Pro Asn Lys Gln Cys Pro Val Gln Gln Cys Phe

275 280 285

Gly Lys Arg Gly Pro Asn Gln Asn Phe Gly Gly Ser Glu Met Leu Lys

290 295 300

Leu Gly Thr Ser Asp Pro Gln Phe Pro Ile Leu Ala Glu Leu Ala Pro

305 310 315 320

Thr Val Gly Ala Phe Phe Phe Gly Ser Lys Leu Glu Leu Val Lys Lys

325 330 335

Asn Ser Gly Gly Ala Asp Glu Pro Thr Lys Asp Val Tyr Glu Leu Gln

340 345 350

Tyr Ser Gly Ala Val Arg Phe Asp Ser Thr Leu Pro Gly Phe Glu Thr

355 360 365

Ile Met Lys Val Leu Asn Glu Asn Leu Asn Ala Tyr Gln Lys Asp Gly

370 375 380

Gly Ala Asp Val Val Ser Pro Lys Pro Gln Arg Lys Gly Arg Arg Gln

385 390 395 400

Ala Gln Glu Lys Lys Asp Glu Val Asp Asn Val Ser Val Ala Lys Pro

405 410 415

Lys Ser Ser Val Gln Arg Asn Val Ser Arg Glu Leu Thr Pro Glu Asp

420 425 430

Arg Ser Leu Leu Ala Gln Ile Leu Asp Asp Gly Val Val Pro Asp Gly

435 440 445

Leu Glu Asp Asp Ser Asn Val

450 455

<210> 5

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<212> DNA

<213> Artificial sequence

<220>

<223> COVAX192_E

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tttcttgctt tcgtggtatt cttgctagtt acactagcca ttcttactgc gcttcgattg 120

tgtgcgtact gttgcaatat tgttaacgtg agtcttgtaa aaccttcttt ttacgtttac 180

tctcgtgtta aaaatctgaa ttcttctcgg gttcctgatc ttctggtcta a 231

<210> 6

<211> 76

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ envelope _ protein _ Sars-CoV2

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Met Val Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn

1 5 10 15

Ser Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu

20 25 30

Ala Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val

35 40 45

Asn Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys

50 55 60

Asn Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val

65 70 75

<210> 7

<211> 255

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX191_E

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atggtgttta atttattcct tacagacaca gtatggtatg tggggcagat tatttttata 60

ttcgcagtgt gtttgatggt caccataatt gtggttgcct tccttgcgtc tatcaaactt 120

tgtattcaac tttgcggttt atgtaatact ttggtgctgt ccccttctat ttatttgtat 180

gataggagta agcagcttta taagtactat aatgaagaaa tgagactgcc cctattagag 240

gtggatgata tctaa 255

<210> 8

<211> 84

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ envelope _ protein _ MHV

<400> 8

Met Val Phe Asn Leu Phe Leu Thr Asp Thr Val Trp Tyr Val Gly Gln

1 5 10 15

Ile Ile Phe Ile Phe Ala Val Cys Leu Met Val Thr Ile Ile Val Val

20 25 30

Ala Phe Leu Ala Ser Ile Lys Leu Cys Ile Gln Leu Cys Gly Leu Cys

35 40 45

Asn Thr Leu Val Leu Ser Pro Ser Ile Tyr Leu Tyr Asp Arg Ser Lys

50 55 60

Gln Leu Tyr Lys Tyr Tyr Asn Glu Glu Met Arg Leu Pro Leu Leu Glu

65 70 75 80

Val Asp Asp Ile

<210> 9

<211> 672

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX192_M

<400> 9

atggtggcag attccaacgg tactattacc gttgaggagc tgaaaaagct ccttgaacaa 60

tggaacctag taataggttt cctattcctt acatggattt gcctgctgca atttgcctat 120

gccaacagga ataggttttt gtacatcatt aagttgattt tcctctggct gttatggcca 180

gtaactttag cttgttttgt gcttgctgct gtttacagaa taaattggat caccggtgga 240

attgctattg caatggcttg tcttgtagga ttgatgtggc taagctactt cattgcttct 300

ttcagactgt ttgcgcgtac gcgttccatg tggtcattca atccagaaac taacattctt 360

ctcaacgtgc cactccatgg aactattctg actagaccgc ttctagaaag tgaactcgta 420

atcggagctg ttatccttcg tggacatctt cgtattgctg gacatcatct aggacgctgt 480

gacatcaagg atctacctaa agaaatcact gttgctacat cacgaacgct ttcttattac 540

aaattgggag cttcacagcg tgtagcaggt gattcaggtt ttgctgcata tagtcgctac 600

aggattggca actataaatt aaacacagac cattccagta gcagtgacaa tattgctttg 660

cttgtacagt aa 672

<210> 10

<211> 223

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ membrane _ protein _ Sars-CoV2

<400> 10

Met Val Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys

1 5 10 15

Leu Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp

20 25 30

Ile Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr

35 40 45

Ile Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala

50 55 60

Cys Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly

65 70 75 80

Ile Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr

85 90 95

Phe Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser

100 105 110

Phe Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr

115 120 125

Ile Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val

130 135 140

Ile Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys

145 150 155 160

Asp Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr

165 170 175

Leu Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser

180 185 190

Gly Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn

195 200 205

Thr Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln

210 215 220

<210> 11

<211> 690

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX191_M

<400> 11

atggtgagta gtactactca ggccccagag cccgtctatc aatggaccgc cgacgaggca 60

gttcaattcc ttaaggaatg gaacttctcg ttgggcatta tactactctt tattactatc 120

atactacagt tcggttacac gagccgtagc atgtttattt atgttgtgaa aatgataatc 180

ttgtggttaa tgtggccact gactattgtt ttgtgtattt tcaattgcgt gtatgcgcta 240

aataatgtgt atcttggatt ttctatagtg tttactatag tgtccattgt aatctggatc 300

atgtattttg tgaacagcat aaggttgttt atcaggactg gtagctggtg gagcttcaac 360

cccgaaacaa acaaccttat gtgtatagat atgaaaggta ccgtgtatgt tagacccatt 420

attgaggatt accatacact aacagccact attattcgtg gccacctcta catgcaaggt 480

gttaagctag gcaccggttt ctctttgtct gacttgcccg cttatgttac agttgctaag 540

gtgtcacacc tttgcactta taagcgcgca ttcttagaca aggtagacgg tgttagcggt 600

tttgctgttt atgtgaagtc caaggtcgga aattaccgac tgccctcaaa caaaccgagt 660

ggcgcggaca ccgcattgtt gagaacctaa 690

<210> 12

<211> 229

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ membrane _ protein _ MHV

<400> 12

Met Val Ser Ser Thr Thr Gln Ala Pro Glu Pro Val Tyr Gln Trp Thr

1 5 10 15

Ala Asp Glu Ala Val Gln Phe Leu Lys Glu Trp Asn Phe Ser Leu Gly

20 25 30

Ile Ile Leu Leu Phe Ile Thr Ile Ile Leu Gln Phe Gly Tyr Thr Ser

35 40 45

Arg Ser Met Phe Ile Tyr Val Val Lys Met Ile Ile Leu Trp Leu Met

50 55 60

Trp Pro Leu Thr Ile Val Leu Cys Ile Phe Asn Cys Val Tyr Ala Leu

65 70 75 80

Asn Asn Val Tyr Leu Gly Phe Ser Ile Val Phe Thr Ile Val Ser Ile

85 90 95

Val Ile Trp Ile Met Tyr Phe Val Asn Ser Ile Arg Leu Phe Ile Arg

100 105 110

Thr Gly Ser Trp Trp Ser Phe Asn Pro Glu Thr Asn Asn Leu Met Cys

115 120 125

Ile Asp Met Lys Gly Thr Val Tyr Val Arg Pro Ile Ile Glu Asp Tyr

130 135 140

His Thr Leu Thr Ala Thr Ile Ile Arg Gly His Leu Tyr Met Gln Gly

145 150 155 160

Val Lys Leu Gly Thr Gly Phe Ser Leu Ser Asp Leu Pro Ala Tyr Val

165 170 175

Thr Val Ala Lys Val Ser His Leu Cys Thr Tyr Lys Arg Ala Phe Leu

180 185 190

Asp Lys Val Asp Gly Val Ser Gly Phe Ala Val Tyr Val Lys Ser Lys

195 200 205

Val Gly Asn Tyr Arg Leu Pro Ser Asn Lys Pro Ser Gly Ala Asp Thr

210 215 220

Ala Leu Leu Arg Thr

225

<210> 13

<211> 3885

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 S

<400> 13

atggtgtttg tttttcttgt tttattgcca ctagtctcta gtcagtgtgt taatcttaca 60

atggtgtttg tttttcttgt tttattgcca ctagtctcta gtcagtgtgt taatcttaca 120

accagaactc aattaccccc tgcatacact aattctttca cacgtggtgt ttattaccct 180

gacaaagttt tcagatcctc agttttacat tcaactcagg acttgttctt acctttcttt 240

tccaatgtta cttggttcca tgctatacat gtctctggga ccaatggtac taagaggttt 300

gataaccctg tcctaccatt taatgatggt gtttactttg cttccactga gaagtctaac 360

ataataagag gctggatttt tggtactact ttagattcga aaacccagtc cctacttatt 420

gttaataacg ctactaatgt tgttatcaaa gtctgtgaat ttcaattttg taacgatcca 480

tttttgggtg tttattacca caaaaacaac aaaagttgga tggaaagtga gttcagagtt 540

tattctagtg cgaataattg cacttttgaa tacgtctctc agccttttct tatggacctt 600

gaaggaaaac agggtaattt caaaaatctt agggaatttg tgttcaagaa tattgatggt 660

tacttcaaga tatactctaa gcacacgcct attaatttag tgcgtgatct ccctcagggt 720

ttttcggctt tagaaccatt ggtagatttg ccaataggta ttaacatcac taggtttcaa 780

actttacttg ctttacatag aagttattta actcctggtg attcttcttc aggttggaca 840

gctggtgctg cagcttatta tgtgggttat cttcaaccta ggacttttct actgaagtac 900

aatgaaaatg gaaccattac agatgctgta gactgtgcac ttgaccctct ctcagaaaca 960

aagtgtacgt tgaaatcctt cactgtagaa aaaggaatct atcaaacttc taactttaga 1020

gtccaaccaa cagaatctat tgttagattt cctaacatca caaacttgtg cccttttggt 1080

gaagttttta acgccaccag atttgcatct gtttatgctt ggaacaggaa gagaatcagc 1140

aactgtgttg ctgattattc tgtcctgtat aattccgcat cattttccac ttttaagtgt 1200

tatggagtgt ctcctactaa attaaatgat ctctgcttta ctaatgtcta tgcagattca 1260

tttgtaatta gaggtgatga agtcagacaa atcgctccag ggcaaactgg aaagattgct 1320

gattataact acaaattacc agatgatttt acaggctgcg ttatagcttg gaattctaac 1380

aatcttgatt ctaaggttgg tggtaattat aattacctgt acagattgtt taggaagtct 1440

aatctcaaac cttttgagag agatatttca actgaaatct atcaggccgg tagcacacct 1500

tgtaatggtg ttgaaggttt taattgttac tttcctctgc aatcatatgg tttccaaccc 1560

actaatggtg ttggttacca accatacaga gtagtagtac tttcttttga acttctacat 1620

gcaccagcaa ctgtttgtgg acctaaaaag tctactaatt tggttaagaa caagtgtgtc 1680

aatttcaact tcaatggttt aacaggcaca ggtgttctta ctgagtctaa caaaaagttt 1740

ctgcctttcc aacaatttgg cagagacatt gctgacacta ctgatgctgt tcgtgatcca 1800

caaacacttg agattcttga cattacacca tgttcttttg gtggtgtcag tgttataaca 1860

ccaggaacaa atacttctaa ccaggttgct gttctttatc aggatgttaa ctgcacagaa 1920

gtccctgttg ctattcatgc agatcaactt actcctactt ggcgtgttta ttctacaggt 1980

tctaatgttt ttcaaacacg tgcaggctgt ttaatagggg ctgaacatgt caacaactca 2040

tatgagtgtg acatacccat tggtgcaggt atatgcgcta gttatcagac tcagactaat 2100

tctcctcgga gagcaagaag tgtagctagt caatccatca ttgcctacac tatgtcactt 2160

ggtgcagaaa attcagttgc ttactctaat aactctattg ccatacccac aaattttact 2220

attagcgtta ccacagaaat tctaccagtg tctatgacca agacatcagt agattgtaca 2280

atgtacattt gtggtgattc aactgaatgc agcaatcttt tgttgcaata tggcagtttt 2340

tgtacacaat taaaccgtgc tttaactgga atagctgttg aacaagacaa aaacacccaa 2400

gaagtttttg cacaagtcaa acaaatttac aagacaccac caattaaaga ttttggcggt 2460

tttaatttta gccagatact gccagatcca tcaaaaccaa gcaagaggtc atttattgaa 2520

gatctactgt tcaacaaagt gacacttgca gatgctggct tcatcaaaca atatggtgat 2580

tgccttggtg atattgctgc tagagacctc atttgtgcac aaaagtttaa cggccttact 2640

gttttgccac ctttgctcac agatgaaatg attgctcaat acacttctgc actgttagca 2700

ggtacaatca cttctggttg gacttttggt gcaggtgctg cattacaaat accatttgct 2760

atgcaaatgg cttataggtt taatggtatt ggagttacac agaatgttct ctatgagaac 2820

caaaaattga ttgccaacca atttaatagt gctattggca aaattcaaga ctcactttct 2880

tccacagcaa gtgcacttgg aaaacttcaa gatgtggtca accaaaatgc acaagcttta 2940

aacacgcttg ttaaacaact tagctccaat tttggtgcaa tttcaagtgt tttaaacgac 3000

atcctttcac gtcttgacaa agttgaggct gaagtgcaaa ttgataggtt gatcacaggc 3060

agacttcaaa gtttgcagac atatgtgact caacaattaa ttagagctgc agaaatcaga 3120

gcttctgcta atcttgctgc tactaaaatg tcagagtgtg tacttggaca atcaaaaaga 3180

gttgactttt gcggaaaggg ctatcatctt atgtcatttc ctcagtcagc acctcatggt 3240

gtcgtctttt tgcatgtgac ttatgtccct gcacaagaaa agaacttcac aactgctcct 3300

gccatttgtc atgatggaaa agcacacttt cctcgtgaag gtgtctttgt ttcaaatggc 3360

acacactggt ttgtaacaca aaggaatttt tatgaaccac aaatcattac tacagacaac 3420

acatttgtgt ctggtaactg tgatgttgta ataggaattg tcaacaacac agtttatgat 3480

cctttgcaac ctgaattaga ctcattcaag gaggagcttg ataaatactt caagaaccat 3540

acctcaccag atgttgattt aggtgacatc tctggcatta atgcttcagt tgtaaacatt 3600

cagaaagaaa tcgaccgcct caatgaggtt gccaagaatt taaatgaatc tctcatcgat 3660

ctccaagaac ttggaaagta tgagcagtat ataaaatggc catggtacat ttggctaggt 3720

tttatagctg gcttgattgc catagtaatg gtgacaatta tgctttgctg tatgaccagt 3780

tgctgtagtt gtctcaaggg ctgttgttct tgtggatcct gctgcaaatt tgacgaggac 3840

gactctgagc cagtgctcaa aggagtcaaa ttacattaca cataa 3885

<210> 14

<211> 1274

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ spike _ protein _ Sars-CoV2

<400> 14

Met Val Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys

1 5 10 15

Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser

20 25 30

Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val

35 40 45

Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr

50 55 60

Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe

65 70 75 80

Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr

85 90 95

Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp

100 105 110

Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val

115 120 125

Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val

130 135 140

Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val

145 150 155 160

Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe

165 170 175

Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu

180 185 190

Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His

195 200 205

Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu

210 215 220

Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln

225 230 235 240

Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser

245 250 255

Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln

260 265 270

Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp

275 280 285

Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu

290 295 300

Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg

305 310 315 320

Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu

325 330 335

Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr

340 345 350

Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val

355 360 365

Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser

370 375 380

Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser

385 390 395 400

Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr

405 410 415

Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly

420 425 430

Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly

435 440 445

Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro

450 455 460

Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro

465 470 475 480

Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr

485 490 495

Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val

500 505 510

Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro

515 520 525

Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe

530 535 540

Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe

545 550 555 560

Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala

565 570 575

Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser

580 585 590

Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln

595 600 605

Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala

610 615 620

Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly

625 630 635 640

Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His

645 650 655

Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys

660 665 670

Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val

675 680 685

Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn

690 695 700

Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr

705 710 715 720

Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser

725 730 735

Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn

740 745 750

Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu

755 760 765

Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala

770 775 780

Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly

785 790 795 800

Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg

805 810 815

Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala

820 825 830

Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg

835 840 845

Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro

850 855 860

Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala

865 870 875 880

Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln

885 890 895

Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val

900 905 910

Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe

915 920 925

Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser

930 935 940

Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu

945 950 955 960

Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser

965 970 975

Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val

980 985 990

Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr

995 1000 1005

Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn

1010 1015 1020

Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg

1025 1030 1035 1040

Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser

1045 1050 1055

Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln

1060 1065 1070

Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala

1075 1080 1085

His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe

1090 1095 1100

Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn

1105 1110 1115 1120

Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn

1125 1130 1135

Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu

1140 1145 1150

Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly

1155 1160 1165

Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile

1170 1175 1180

Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp

1185 1190 1195 1200

Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr

1205 1210 1215

Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr

1220 1225 1230

Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys

1235 1240 1245

Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro

1250 1255 1260

Val Leu Lys Gly Val Lys Leu His Tyr Thr

1265 1270

<210> 15

<211> 21746

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX_Syn_RepA56

<400> 15

gtataagagt gattggcgtc cgtacgtacc ctctcaactc taaaactctt gtagtttaaa 60

tctaatctaa actttataaa cggcacttcc tgcgtgtcca tgcccgcggg cctggtcttg 120

tcatagtgct gacatttgta gttccttgac tttcgttctc tgccagtgac gtgtccattc 180

ggcgccagca gcccacccat aggttgcata atggcaaaga tgggcaaata cggcctgggc 240

ttcaaatggg ccccagaatt tccatggatg cttccgaacg catcggagaa gttgggtaac 300

cctgagaggt cagaggagga tgggttttgc ccctctgctg cgcaagaacc gaaagttaaa 360

ggaaaaactt tggttaatca cgtgagggtg aattgtagcc ggcttccagc tttggaatgc 420

tgtgttcagt ctgccataat ccgtgatatt tttgtagatg aggatcccca gaaggtggag 480

gcctcaacta tgatggcatt gcagttcggt agtgccgtct tggttaagcc atccaagcgc 540

ttgtctattc aggcatggac taatttgggt gtgcttccca aaacagctgc catggggttg 600

ttcaagcgcg tctgcctgtg taacaccagg gagtgctctt gtgacgccca cgtggccttt 660

caccttttta cggtccaacc cgatggtgta tgcctgggta atggccgttt tataggctgg 720

ttcgttccag tcacagccat accggagtat gcgaagcagt ggttgcaacc ctggtccatc 780

cttcttcgta agggtggtaa caaagggtct gtgacatccg gccacttccg ccgcgctgtt 840

accatgcctg tgtatgactt taatgtagag gatgcttgtg aggaggttca tcttaacccg 900

aagggtaagt actcctgcaa ggcgtatgcc ctgctgaagg gctatcgcgg tgttaagccc 960

atcctgtttg tggaccagta tggttgcgac tatactggat gtctcgccaa gggtcttgag 1020

gactatggcg atctcacctt gagtgagatg aaggagttgt tccctgtgtg gcgtgactcc 1080

ttggatagtg aagtccttgt ggcttggcac gttgatcgag atcctcgggc tgctatgcgt 1140

ctgcagactc ttgctactgt acgttgcatt gattatgtgg gccaaccgac cgaggatgtg 1200

gtggatggag atgtggtagt gcgtgagcct gctcatcttc tcgcagccaa tgccattgtt 1260

aaaagactcc cccgtttggt ggagactatg ctgtatacgg attcgtccgt tacagaattc 1320

tgttataaaa ccaagctgtg tgaatgcggt tttatcacgc agtttggcta tgtggattgt 1380

tgtggtgaca cctgtgattt tcgtgggtgg gttgccggca atatgatgga tggctttcca 1440

tgtccagggt gtaccaaaaa ttatatgccc tgggaattgg aggcccagtc atcaggtgtt 1500

ataccagaag gaggtgttct attcactcag agcactgata cagtgaatcg tgagtccttt 1560

aagctctacg gtcatgctgt tgtgcctttt ggttctgctg tgtattggag cccttgccca 1620

ggtatgtggc ttccagtaat ttggtcgtcg gttaagtcat actctggttt gacttataca 1680

ggagtagttg gttgtaaggc aattgttcaa gagacagacg ctatatgtcg ttctctgtat 1740

atggattatg tccagcacaa gtgtggcaat ctcgagcaga gagctatcct tggattggac 1800

gatgtctatc atagacagtt gcttgtgaat aggggtgact atagtctcct ccttgagaat 1860

gtggatttgt ttgttaagcg gcgcgctgaa tttgcttgca aattcgccac ctgtggagat 1920

ggtcttgtac ccctcctact agatggttta gtgccccgca gttattattt gattaagagt 1980

ggtcaagctt tcacctctat gatggttaat tttagccatg aggtgactga catgtgtatg 2040

gacatggctt tattgttcat gcatgatgtt aaagtggcca ctaagtatgt taagaaggtt 2100

actggcaaac tggccgtgcg ctttaaagcg ttgggtgtag ccgttgtcag aaaaattact 2160

gaatggtttg atttagccgt ggacattgct gctagtgccg ctggatggct ttgctaccag 2220

ctggtaaatg gcttatttgc agtggccaat ggtgttataa cctttgtaca ggaggtgcct 2280

gagcttgtca agaattttgt tgacaagttc aaggcatttt tcaaggtttt gatcgactct 2340

atgtcggttt ctatcttgtc tggacttact gttgtcaaga ctgcctcaaa tagggtgtgt 2400

cttgctggca gtaaggttta tgaagttgtg cagaaatctt tgtctgcata tgttatgcct 2460

gtgggttgca gcgaagccac ttgtttggtg ggtgagattg aacctgcagt ttttgaagat 2520

gatgttgttg atgtggttaa agccccatta acatatcaag gctgttgtaa gccacccact 2580

tctttcgaga agatttgtat tgtggataaa ttgtatatgg ccaagtgtgg tgatcaattt 2640

taccctgtgg ttgttgataa cgacactgtt ggcgtgttag atcagtgctg gaggtttccc 2700

tgtgcgggca agaaagtcga gtttaacgac aagcccaaag tcaggaagat accctccacc 2760

cgtaagatta agatcacctt cgcactggat gcgacctttg atagtgttct ttcgaaggcg 2820

tgttcagagt ttgaagttga taaagatgtt acattggatg agctgcttga tgttgtgctt 2880

gacgcagttg agagtacgct cagcccttgt aaggagcatg atgtgatagg cacaaaagtt 2940

tgtgctttac ttgataggtt ggcaggagat tatgtctatc tttttgatga gggaggcgat 3000

gaagtgatcg ccccgaggat gtattgttcc ttttctgctc ctgatgacga ggactgcgtt 3060

gcagcggatg ttgtagatgc agatgaaaac caagatgatg atgccgagga ctcagcagtc 3120

cttgtcgctg atacccaaga agaggacggc gttgccaagg ggcaggttga ggcggattcg 3180

gaaatttgcg ttgcgcatac tggtagtcaa gaagaattgg ctgagcctga tgctgtcgga 3240

tctcaaactc ccatcgcctc tgctgaggaa accgaagtcg gagaggcaag cgacagggaa 3300

gggattgctg aggcgaaggc aactgtgtgt gctgatgctg tagatgcctg ccccgatcaa 3360

gtggaggcat ttgaaattga aaaggtcgag gactctatct tggatgagct tcaaactgaa 3420

cttaatgcgc cagcggacaa gacctatgag gatgtcttgg cattcgatgc cgtatgctca 3480

gaggcgttgt ctgcattcta tgctgtgccg agtgatgaga cgcactttaa agtgtgtgga 3540

ttctattcgc ctgctataga gcgcactaat tgttggctgc gttctacttt gatagtaatg 3600

cagagtctac ctttggaatt taaagacttg gagatgcaaa agctctggtt gtcttacaag 3660

gccggctatg accaatgctt tgtggacaaa ctagttaaga gcgtgcccaa gtctattatc 3720

cttccacaag gtggttatgt ggcagatttt gcctatttct ttctaagcca gtgtagcttt 3780

aaagcttatg ctaactggcg ttgtttagag tgtgacatgg agttaaagct tcaaggcttg 3840

gacgccatgt ttttctatgg ggacgttgtg tctcatatgt gcaagtgtgg taatagcatg 3900

accttgttgt ctgcagatat accctacact ttgcattttg gagtgcgaga tgataagttt 3960

tgcgcttttt acacgccaag aaaggtcttt agggctgctt gtgcggtaga tgttaatgat 4020

tgtcactcta tggctgtagt agagggcaag caaattgatg gtaaagtggt taccaaattt 4080

attggtgaca aatttgattt tatggtgggt tacgggatga catttagtat gtctcctttt 4140

gaactcgccc agttatatgg ttcatgtata acaccaaatg tttgttttgt taaaggagat 4200

gttataaagg ttgttcgctt agttaatgct gaagtcattg ttaaccctgc taatgggcgt 4260

atggctcatg gtgccggcgt cgccggcgcc atagctgaaa aggcgggcag tgcttttatt 4320

aaagaaacct ccgatatggt gaaggctcag ggcgtttgcc aggttggtga atgctatgaa 4380

tctgccggtg gtaagttatg taaaaaggtg cttaacattg tagggccaga tgcgcgaggg 4440

catggcaagc aatgctattc acttttagag cgtgcttatc agcatattaa taagtgtgac 4500

aatgttgtca ctactttaat ttcggctggt atatttagtg tgcctactga tgtctcccta 4560

acttacttac ttggtgtagt gacaaagaat gtcattcttg tcagtaacaa ccaggatgat 4620

tttgatgtga tagagaagtg tcaggtgacc tccgttgctg gtaccaaagc gctatcactt 4680

caattggcca aaaatttgtg ccgtgatgta aagtttgtga cgaatgcatg tagttcgctt 4740

tttagtgaat cttgctttgt ctcaagctat gatgtgttgc aggaagttga agcgctgcga 4800

catgatatac aattggatga tgatgctcgt gtctttgtgc aggctaatat ggactgtctg 4860

cccacagact ggcgtctcgt taacaaattt gatagtgttg atggtgttag aaccattaag 4920

tattttgaat gcccgggcgg gatttttgta tccagccagg gcaaaaagtt tggttatgtt 4980

cagaatggtt catttaagga ggcgagtgtt agccaaataa gggctttact cgctaataag 5040

gttgatgtct tgtgtactgt tgatggtgtt aacttccgct cctgctgcgt agcagagggt 5100

gaagtttttg gcaagacatt aggttcagtc ttttgtgatg gcataaatgt caccaaagtt 5160

aggtgtagtg ccatttacaa gggtaaggtt ttctttcagt acagtgattt gtccgaggca 5220

gatcttgtgg ctgttaaaga tgcctttggt tttgatgaac cacaactgct gaagtactac 5280

actatgcttg gcatgtgtaa gtggccagta gttgtttgtg gcaattattt tgctttcaag 5340

cagtcaaata ataattgcta catcaacgtg gcatgtttaa tgctgcaaca cttgagttta 5400

aagtttccta agtggcaatg gcaagaggct tggaacgagt tccgctctgg taaaccacta 5460

aggtttgtgt ccttggtatt agcaaagggc agctttaaat ttaatgaacc ttctgattct 5520

atcgatttta tgcgtgtggt gctacgtgaa gcagatttga gtggtgccac gtgcaatttg 5580

gaatttgttt gtaaatgtgg tgtgaagcaa gagcagcgca aaggtgttga cgctgttatg 5640

cattttggta cgttggataa aggtgatctt gtcaggggtt ataatatcgc atgtacgtgc 5700

ggtagtaaac ttgtgcattg cacccaattt aacgtaccat ttttaatttg ctccaacaca 5760

ccagagggta ggaaactgcc cgacgatgtt gttgcagcta atatttttac tggtggtagt 5820

gtgggccatt acacgcatgt gaaatgtaaa cccaagtacc agctttatga tgcttgtaat 5880

gttaataagg tttcggaggc taagggtaat tttaccgatt gcctctacct taaaaattta 5940

aagcaaacct tctcgtctgt gctgacgact ttttatttag atgacgtaaa gtgtgtggag 6000

tataagccag atttatcgca gtattactgt gagtctggta aatattatac aaaacccatt 6060

attaaggccc aatttagaac atttgagaag gttgatggtg tctataccaa ctttaaattg 6120

gtgggacata gtattgctga aaaactcaat gctaagctgg gatttgattg taattctccc 6180

tttgtggagt ataaaattac agagtggcca acagctactg gagatgtggt gttggctagt 6240

gatgatttgt atgtaagtcg gtacttaagc gggtgcatta cttttggtaa accggttgtc 6300

tggcttggcc atgaggaagc atcgctgaaa tctctcacat attttaatag acctagtgtc 6360

gtttgtgaaa ataaatttaa cgtgttgccc gttgatgtca gtgaacccac ggacaagggg 6420

cctgtgcctg ctgcagtcct tgttaccggc gtccctggag ctgatgcgtc agctggtgcc 6480

ggtattgcca aggagcaaaa agcctgtgct tctgctagtg tggaggatca ggttgttacg 6540

gaggttcgtc aagagccatc tgtttcagct gctgatgtca aagaggttaa attgaatggt 6600

gttaaaaagc ctgttaaggt ggaaggtagt gtggttgtta atgatcccac tagcgaaacc 6660

aaagttgtta aaagtttgtc tattgttgat gtctatgata tgttcctgac agggtgtaag 6720

tatgtggttt ggactgctaa tgagttgtct cgactagtaa attcaccgac tgttagggag 6780

tatgtgaagt ggggtatggg aaagattgta acacccgcta agttgttgtt gttaagagat 6840

gagaagcaag agttcgtagc gccaaaagta gtcaaggcga aagctattgc ctgctattgt 6900

gctgtgaagt ggtttctcct ctattgtttt agttggataa agtttaatac tgacaataag 6960

gttatataca ccacagaagt agcttcaaag cttactttca agttgtgctg tttggccttt 7020

aagaatgcct tacagacgtt taattggagc gttgtgtcta ggggcttttt cctagttgca 7080

acggtctttt tactctggtt taactttttg tatgctaatg ttattttgag tgacttctat 7140

ttgcctaata ttgggcctct ccctacgttt gtgggacaga tagttgcgtg gtttaagact 7200

acatttggtg tgtcaaccat ctgtgatttc taccaggtga cggatttggg ctatagaagt 7260

tcgttttgta atggaagtat ggtatgtgaa ctatgcttct caggttttga tatgctggac 7320

aactatgatg ctataaatgt tgttcaacac gttgtagata ggcgtttgtc ctttgactat 7380

attagcctat ttaaactggt agttgagctt gtaatcggct actctcttta tactgtgtgc 7440

ttctacccac tgtttgtcct tattggaatg cagttattga ccacatggtt gcctgaattc 7500

tttatgctgg agactatgca ttggagtgct cgtttgtttg tgtttgttgc caatatgctt 7560

ccagctttta cgttactgcg attttacatc gtggtgacag ctatgtataa ggtctattgt 7620

ctttgtagac atgttatgta tggatgtagt aagcctggtt gcttgttttg ttataagaga 7680

aaccgtagtg tccgtgttaa gtgtagcacc gttgttggtg gttcactacg ctattacgat 7740

gtaatggcta acggcggcac aggtttctgt acaaagcacc agtggaactg tcttaattgc 7800

aattcctgga aaccaggcaa tacattcata actcatgaag cagcggcgga cctctctaag 7860

gagttgaaac gccctgtgaa tccaacagat tctgcttatt actcggtcac agaggttaag 7920

caggttggtt gttccatgcg tttgttctac gagagagatg gacagcgtgt ttatgatgat 7980

gttaatgcta gtttgtttgt ggacatgaat ggtctgctgc attctaaagt taaaggtgtg 8040

cctgaaacgc atgttgtggt tgttgagaat gaagctgata aagctggttt tctcggcgcc 8100

gcagtgtttt atgcacaatc gctctacaga cctatgttga tggtggaaaa gaaattaata 8160

actaccgcca acactggttt gtctgttagt cgaactatgt ttgaccttta tgtagattca 8220

ttgctgaacg tcctcgacgt ggatcgcaag agtctaacaa gttttgtaaa tgctgcgcac 8280

aactctctaa aggagggtgt tcagcttgaa caagttatgg atacctttat tggctgtgcc 8340

cgacgtaagt gtgctataga ttctgatgtt gaaaccaagt ctattaccaa gtccgtcatg 8400

tcggcagtaa atgctggcgt tgattttacg gatgagagtt gtaataactt ggtgcctacc 8460

tatgttaaaa gtgacactat cgttgcagcc gatttgggtg ttcttattca gaataatgct 8520

aagcatgtac aggctaatgt tgctaaagcc gctaatgtgg cttgcatttg gtctgtggat 8580

gcttttaacc agctatctgc tgacttacag cataggctgc gaaaagcatg ttcaaaaact 8640

ggcttgaaga ttaagcttac ttataataag caggaggcaa atgttcctat tttaactaca 8700

ccgttctctc ttaaaggggg cgctgttttt agtagaatgt tacaatggtt gtttgttgct 8760

aatttgattt gtttcattgt gttgtgggcc cttatgccaa catatgcagt gcacaaatcg 8820

gatatgcagt tgcctttata tgccagtttt aaagttatag ataacggtgt gctaagggat 8880

gtgtctgtta ctgacgcatg cttcgcaaac aaatttaatc aattcgacca atggtatgag 8940

tctacttttg gtcttgctta ttaccgcaac tctaaggctt gtcctgttgt ggttgctgta 9000

atagatcaag acattggcca taccttattt aatgttccta ccacagtttt aagatatgga 9060

tttcatgtgt tgcattttat aacccatgca tttgctactg atagcgtgca gtgttacacg 9120

ccacatatgc aaatccccta tgataatttc tatgctagtg gttgcgtgtt gtcatccctc 9180

tgtactatgc ttgcgcatgc agatggaacc ccgcatcctt attgttatac agggggtgtt 9240

atgcataatg cctctctgta tagttctttg gctcctcatg tccgttataa cctggctagt 9300

tcaaatggtt atatacgttt tcccgaagtg gttagtgaag gcattgtgcg tgttgtgcgc 9360

actcgctcta tgacctactg cagggttggt ttatgtgagg aggccgagga gggtatctgc 9420

tttaatttta atcgttcatg ggtattgaac aacccgtatt atagggccat gcctggaact 9480

ttttgtggta ggaatgcttt tgatttaata catcaagttt taggaggatt agtgcggcct 9540

attgatttct ttgccttaac ggcgagttca gtggctggtg ctatccttgc aattattgtc 9600

gttttggctt tctattattt aatcaagctt aagcgtgcct ttggtgacta cactagtgtt 9660

gtggttatca atgtaattgt gtggtgtata aattttctga tgctttttgt gtttcaggtt 9720

tatcccacat tgtcttgttt atatgcttgt ttctacttct acaccacgct ttatttccct 9780

tcggagataa gtgttgttat gcatttgcaa tggcttgtca tgtatggtgc tattatgccc 9840

ttgtggtttt gcattattta cgtggcagtc gttgtttcaa accatgcatt gtggttgttc 9900

tcttactgcc gcaaaattgg taccgaggtt cgtagtgacg gcacatttga ggaaatggcc 9960

cttactacct ttatgattac taaagaatct tattgtaagt tgaaaaactc tgtttctgat 10020

gttgctttta acaggtactt gagtctttac aacaagtacc gttacttcag tggcaaaatg 10080

gatactgccg cttatagaga ggctgcctgt tcacaactgg caaaggcaat ggaaacattt 10140

aaccataata atggtaatga tgttctctat cagcctccaa ccgcctctgt tactacatca 10200

tttttacagt ctggtatagt gaagatggtg tcgcccacct ctaaagtgga gccttgtatt 10260

gttagtgtta cttatggtaa catgacactt aatgggttgt ggttggatga taaagtttat 10320

tgcccaagac atgttatctg ttcttcagct gacatgacag accctgatta tcctaatttg 10380

ctttgtagag tgacatcaag tgatttttgt gttatgtctg gtcgtatgag ccttactgta 10440

atgtcttatc aaatgcaggg ctgccaactt gttttgactg ttacactgca aaatcctaac 10500

acgcctaagt attccttcgg tgttgttaag cctggtgaga catttactgt actggctgca 10560

tacaatggca gacctcaagg agccttccat gttacgcttc gtagtagcca taccataaag 10620

ggctcctttc tatgtggatc ctgcggttct gtaggatatg ttttaactgg cgatagtgta 10680

cgatttgttt atatgcatca gctagagttg agtactggtt gtcataccgg tactgacttt 10740

agtgggaact tttatggtcc ctatagagat gcgcaagttg tacaattgcc tgttcaggat 10800

tatacgcaga ctgttaatgt tgtagcttgg ctttatgctg ctatttttaa cagatgcaac 10860

tggtttgtgc aaagtgatag ttgttccctg gaggagttta atgtttgggc tatgaccaat 10920

ggttttagct caatcaaagc cgatcttgtc ttggatgcgc ttgcttctat gacaggcgtt 10980

acagttgaac aggtgttggc cgctattaag aggctgcatt ctggattcca gggcaaacaa 11040

attttaggta gttgtgtgct tgaagatgag ctgacaccaa gtgatgttta tcaacaacta 11100

gctggtgtca agctacagtc aaagcgcaca agagttataa aaggtacatg ttgctggata 11160

ttggcttcaa cgtttttgtt ctgtagcatt atctcagcat ttgtaaaatg gactatgttt 11220

atgtatgtta ctacccatat gttgggagtg acattgtgtg cactttgttt tgtaagcttt 11280

gctatgttgt tgatcaagca taagcatttg tatttaacta tgtacatcat gcctgtgtta 11340

tgcacactgt tttacaccaa ctatttggtt gtgtacaaac agagttttag aggtctagct 11400

tatgcttggc tttcacactt tgtccctgct gtagattata catatatgga tgaagtttta 11460

tatggtgttg tgttgctagt agctatggtg tttgttacca tgcgtagcat aaaccacgac 11520

gtcttttcta ttatgttctt ggttggtaga cttgtcagcc tggtatccat gtggtatttt 11580

ggagccaatt tagaggaaga ggtactattg ttcctcacat ccctatttgg cacgtacaca 11640

tggactacta tgttgtcatt ggctaccgct aaggttattg ctaaatggtt ggctgtgaat 11700

gtcttgtact tcacagacgt accgcaaatt aaattagttc tgttgagcta cttgtgtatt 11760

ggttatgtgt gttgttgtta ttggggaatc ttgtcactcc ttaatagcat ttttaggatg 11820

ccattgggcg tctacaatta taaaatctcc gttcaggagt tacgttatat gaatgctaat 11880

ggcttgcgcc cacctagaaa tagttttgag gccctgatgc ttaattttaa gctgttggga 11940

attggtggtg tgccagtcat tgaagtatct caaattcaat caagattgac ggatgttaaa 12000

tgtgctaatg ttgtgttgct taattgcctc cagcacttgc atattgcatc taattctaag 12060

ttgtggcagt attgtagtac tttgcacaat gaaatactgg ctacatctga tttgagcgtg 12120

gccttcgata agttggctca actcttagtt gttttatttg ctaatccagc agcagtggat 12180

agcaagtgcc ttgcaagtat tgaagaagtg agcgatgatt acgttcgcga caatactgtc 12240

ttgcaagcct tacagagtga atttgttaat atggctagct tcgttgagta tgaacttgct 12300

aagaagaatc tagatgaggc taaggctagc ggctctgcca atcaacagca gattaagcag 12360

ctagagaagg cgtgtaatat tgctaagtca gcatatgagc gcgacagagc tgttgctcgt 12420

aagctggaac gtatggctga tttagctctt acaaacatgt ataaagaagc tagaattaat 12480

gataagaaga gtaaggtagt gtctgcattg caaaccatgc tctttagtat ggtgcgtaag 12540

ctagataacc aagctcttaa ttctatttta gacaacgcag ttaagggttg tgtacctttg 12600

aatgcaatac catcattgac ttcgaacact ctgactataa tagtgccaga taagcaggtt 12660

tttgatcagg ttgtggataa tgtgtatgtc acctatgctg ggaatgtatg gcatatacag 12720

tttattcaag atgctgatgg tgctgttaaa caattgaatg agatagatgt taattcaacc 12780

tggcctctag tcattgctgc aaataggcat aatgaagtgt ctactgttgt tttgcagaac 12840

aatgagttga tgcctcagaa gttgagaact caggttgtca atagtggctc agatatgaat 12900

tgtaatactc ctacccagtg ttactataat actactggca cgggtaagat tgtgtatgct 12960

atacttagtg actgtgacgg cctgaagtac actaagatag taaaagaaga tggaaattgt 13020

gttgttttgg aattggatcc tccctgtaag ttttctgttc aggatgtgaa gggccttaaa 13080

attaagtacc tttactttgt gaaggggtgt aatacactgg ctagaggctg ggttgtaggc 13140

accttatcct cgacagtgag attgcaggcg ggtacggcaa ctgagtatgc ctccaactct 13200

gcaatactgt cgctgtgtgc gttttctgta gatcctaaga aaacgtactt ggattatata 13260

aaacagggtg gagttcccgt tactaattgt gttaagatgt tatgtgacca tgctggcact 13320

ggtatggcca ttactattaa gccggaggca accactaatc aggattctta tggtggtgct 13380

tccgtttgta tatattgccg ctcgcgtgtt gaacatccag atgttgatgg attgtgcaaa 13440

ttacgcggca agtttgtcca agtgccctta ggcataaaag atcctgtgtc atatgtgttg 13500

acgcatgatg tttgtcaggt ttgtggcttt tggcgagatg gtagctgttc ctgtgtaggc 13560

acaggctccc agtttcagtc aaaagacacg aactttttaa acggattcgg ggtacaagtg 13620

taaatgcccg tcttgtaccc tgtgccagtg gcttggacac tgatgttcaa ttaagggcat 13680

ttgacatttg taatgctaat cgagctggca ttggtttgta ttataaagtg aattgctgcc 13740

gcttccagcg tgtagatgag gacggcaaca agttggataa gttctttgtt gttaaaagaa 13800

ctaatttaga agtgtataac aaggagaaag aatgctatga gttgacaaaa gaatgcggtg 13860

ttgtggctga acacgagttc ttcacatttg atgtggaggg aagtcgggta ccacacatag 13920

tccgtaaaga tctttcaaag tttactatgt tagatctttg ctatgcattg cgtcattttg 13980

accgcaatga ttgttcaact cttaaggaaa ttctccttac atatgctgag tgtgaagagt 14040

cctacttcca aaagaaggac tggtatgatt ttgttgagaa tcctgatata attaatgtgt 14100

acaagaagct tggtcctata tttaatagag ccctgcttaa cactgccaag tttgcagacg 14160

cattagtgga ggcaggctta gtaggtgttt taacacttga taatcaagat ttatatggtc 14220

aatggtatga ctttggagat tttgtcaaga cagtacctgg ttgtggtgtt gccgtggcag 14280

actcttatta ttcatatatg atgccaatgc tgactatgtg tcatgcgttg gatagtgagt 14340

tgtttgttaa tggtacttat agggagtttg accttgttca gtatgatttt actgatttca 14400

agctagagct gttcactaag tattttaagc attggagtat gacctaccac ccgaacacct 14460

gtgagtgcga ggatgacagg tgcattattc attgcgccaa ttttaatata cttttcagca 14520

tggtcttacc taagacctgt tttgggcctc ttgttaggca gatatttgtg gatggtgttc 14580

ctttcgttgt gtcgatcggt taccattata aagaattagg tgttgttatg aatatggatg 14640

tggatacaca tcgttatcgc ttgtctctta aggacttgct tttgtatgct gcagaccctg 14700

cccttcatgt ggcgtctgct agtgcactgc ttgatttgcg cacatgttgt tttagcgttg 14760

cagctattac aagtggcgta aaatttcaaa cagttaaacc tggaaatttt aatcaggatt 14820

tctacgagtt tattttgagt aaaggcctgc ttaaagaggg gagctccgtt gatttgaagc 14880

acttcttctt tacgcaggat ggtaatgctg ctattactga ttacaattac tacaagtata 14940

atctacccac catggtggat attaagcagt tgttgtttgt tttagaagtt gttaataagt 15000

acttcgagat ctatgagggt gggtgtatac ccgcaacaca ggtcattgtt aataattatg 15060

acaagagtgc tggctatcca tttaataaat ttggaaaggc caggctctat tatgaggcat 15120

tatcatttga ggagcaggat gaaatttatg cgtataccaa acgcaatgtc ctgccgaccc 15180

taactcaaat gaatcttaaa tatgctatta gtgctaagaa tagggcccgc accgttgctg 15240

gtgtctctat tctcagtact atgactggca gaatgtttca tcaaaagtgt ctaaagagta 15300

tagcagctac tcgcggtgtt cctgtagtta taggcaccac gaagttctat ggcggttggg 15360

atgatatgtt acgccgcctt attaaagatg ttgatagtcc tgtactcatg ggttgggact 15420

atcctaaatg tgatcgtgct atgccaaaca tactgcgtat tgttagtagt ttggtgctag 15480

cccgtaaaca tgattcgtgc tgttcgcata cggatagatt ctatcgtctt gcgaacgagt 15540

gcgcccaagt tttgagtgaa attgttatgt gtggtggttg ttattatgtt aaaccaggtg 15600

gcactagtag tggggatgca accactgctt ttgctaattc tgtgtttaac atttgtcaag 15660

ctgtttccgc caatgtatgc tcgcttatgg catgcaatgg acacaaaatt gaagatttga 15720

gtatacgcga gttacaaaag cgcctatact ctaatgtcta tcgtgcggac catgttgacc 15780

ccgcatttgt tagtgagtat tatgagtttt taaacaagca ttttagtatg atgattttga 15840

gtgatgatgg tgttgtgtgt tataattcag agtttgcgtc caagggttat attgctaata 15900

taagtgcctt tcaacaggta ttatattatc aaaacaacgt gtttatgtct gaggccaaat 15960

gttgggtaga aacagacatc gaaaagggac cgcatgaatt ttgttctcaa catacaatgc 16020

tagtcaagat ggatggtgat gaagtctacc ttccataccc tgatccttcg agaatcttag 16080

gagcaggctg ttttgttgat gatttactca agactgatag cgttctcttg atagagcgtt 16140

tcgtaagtct tgcaattgat gcttatcctt tagtatacca tgagaaccca gagtatcaaa 16200

atgtgttccg ggtatattta gaatacatca agaagctgta caatgatctc ggtaatcaga 16260

tcctggacag ctacagtgtt attttaagta cttgtgatgg tcaaaagttt actgacgaga 16320

cgttttacaa gaacatgtat ttaagaagtg cagtgctgca aagcgttggt gcctgcgttg 16380

tctgtagttc tcaaacatca ttacgttgtg gcagttgcat acgcaagcct ttgctgtgtt 16440

gcaaatgcgc ctatgatcat gttatgtcca ctgatcataa atatgtcctg agtgtgtcac 16500

catatgtgtg taattcaccg ggatgtgatg taaatgatgt taccaaattg tatttaggtg 16560

gtatgtcata ttattgtgag gaccataaac cacagtattc attcaaattg gtgatgaatg 16620

gtatggtttt tggtttatat aagcagtctt gtactggttc gccctacata gaggatttta 16680

ataaaatcgc tagttgcaaa tggacagaag tcgatgatta tgtgctagct aatgaatgca 16740

ccgaacgcct taaattgttt gccgcagaaa cgcagaaggc cacagaagag gcctttaagc 16800

aatgttatgc gtcagcaacg atccgtgaga tcgtgagcga tcgggagtta attttatctt 16860

gggaaattgg taaagtccgc ccgccactta ataaaaatta cgtgttcacc ggctaccatt 16920

ttactaataa tggtaagaca gttttaggtg agtatgtttt tgataagagt gagttgacta 16980

atggtgtgta ttatcgcgcc acaaccactt ataagttatc tgtaggtgat gtgttcattt 17040

taacatcaca cgcagtgtct agtttaagtg ctcctacatt agtaccgcag gagaattata 17100

ctagcattcg ttttgctagt gtttatagtg tgcctgagac gtttcagaat aatgtgccta 17160

attatcagca cattggaatg aagcgctatt gtactgtaca gggaccgcct ggtactggta 17220

agtcccatct agccattggg ctagctgttt attattgtac agcgcgcgtg gtgtataccg 17280

ctgctagcca tgctgcagtt gacgcgctgt gtgaaaaggc acataaattt ctcaacatca 17340

acgactgcac gcgtattgtt cctgcaaagg tgcgtgtaga ttgttatgat aaattcaagg 17400

tcaatgacac cactcgcaag tatgtgttta ctacaataaa tgcattacct gagttggtga 17460

ctgacattat tgtcgttgat gaagttagta tgcttaccaa ctatgagctg tctgttatta 17520

acagtcgtgt tagggctaag cattatgtgt atattggcga cccggcgcag ttacctgcac 17580

cacgtgtgct actgaataag ggaactctag aacctagata ttttaattcc gttaccaagc 17640

taatgtgttg tttgggtcca gatattttct tgggcacctg ttatagatgc cctaaggaga 17700

ttgtggatac ggtgtcagcc ttggtttata ataataagct gaaggctaaa aatgataata 17760

gctccatgtg ctttaaggtt tattataagg gccagactac acatgagagt tctagtgctg 17820

ttaatatgca gcaaatacat ttaatttcca agtttctgaa ggcaaacccc agttggagta 17880

acgccgtatt tattagtcct tataactcgc agaactatgt tgctaagaga gtcttgggat 17940

tacaaaccca gacagtagac tcagcgcagg gttctgaata tgattttgtt atctactcac 18000

agactgcgga aacagcgcat tctgtcaatg taaatagatt caatgttgct attacacgtg 18060

ctaagaaggg tattctctgt gtcatgagta gtatgcaatt atttgagtct cttaatttta 18120

ctacactgac gttggataag attaacaatc cacgattaca gtgtactaca aatttgttta 18180

aggattgtag caggagctat gtaggatatc acccagccca tgcaccatcc tttttggcag 18240

ttgatgacaa atataaggta ggcggtgatt tagccgtttg ccttaatgtt gctgattctg 18300

ctgtcactta ttcgcggctt atatcactca tgggattcaa gcttgacttg acccttgatg 18360

gttattgtaa gctgtttata actagagatg aagctatcaa acgtgttaga gcctgggttg 18420

gcttcgatgc agaaggtgcc catgcgatac gtgatagcat tgggacaaat ttcccattac 18480

aattaggctt ttcgactgga attgattttg ttgtcgaagc cactggaatg tttgctgaga 18540

gagatggtta tgtctttaaa aaggcagccg cacgagctcc tcctggcgaa caatttaaac 18600

accttatccc acttatgtca agagggcaga aatgggatgt ggttcgcatt agaatagtac 18660

aaatgttgtc agaccaccta gtggatttgg cagacagtgt tgtacttgtg acgtgggctg 18720

ccagctttga gctcacatgt ttgcgatatt tcgctaaagt tggaagagaa gttgtgtgta 18780

gtgtctgcac caagcgtgcg acatgtttta attctagaac tggatactat ggatgctggc 18840

gacatagtta ttcctgtgat tacctgtaca acccactaat agttgacatt caacagtggg 18900

gatatacagg atctttaact agcaatcatg atcctatttg cagcgtgcat aagggtgctc 18960

atgttgcatc atctgatgct atcatgaccc ggtgtctagc tgttcatgat tgcttttgta 19020

agtctgttaa ttggaattta gaatacccca ttatttcaaa tgaggtcagt gttaatacct 19080

cctgcaggtt attgcagcgc gtaatgttta gggctgcgat gctatgcaat aggtatgatg 19140

tgtgttatga cattggcaac cctaaaggtc ttgcctgtgt caaaggatat gattttaagt 19200

tctatgacgc ctcccctgtt gttaagtctg ttaaacagtt tgtttacaaa tacgaggcac 19260

ataaagatca atttttagat ggtttgtgta tgttttggaa ctgcaatgtg gataagtatc 19320

cagcgaatgc agttgtgtgt aggtttgaca cgcgtgtgtt gaacaaatta aatctccctg 19380

gctgtaatgg tggcagtttg tatgttaaca aacatgcatt ccacaccagt ccctttaccc 19440

gggctgcctt cgagaatttg aagcctatgc ctttctttta ttattcagat acgccctgtg 19500

tgtatatgga aggcatggaa tctaagcagg tcgattatgt cccattgaga agcgctacat 19560

gcatcacaag atgcaattta ggtggcgctg tttgtttaaa acatgctgag gagtatcgtg 19620

agtaccttga gtcttacaat acggcaacca cagcgggttt tactttttgg gtctataaga 19680

cttttgattt ttacaacctt tggaatactt ttactaggct ccaaagttta gaaaatgtag 19740

tgtataacct ggtcaacgct ggacactttg atggccgggc gggtgaactg ccttgtgctg 19800

ttataggtga gaaagtcatt gccaagattc aaaatgagga tgtcgtggtc tttaaaaata 19860

acacgccatt ccccactaat gtggctgtcg aattatttgc taagcgcagt attcggcccc 19920

accccgagct taagctcttt agaaatttga atattgacgt gtgctggagt cacgtccttt 19980

gggattatgc taaggatagt gtgttttgca gttcgacgta taaggtctgc aaatacacag 20040

atttacagtg cattgaaagc ttgaatgtac tttttgatgg tcgtgataat ggtgctcttg 20100

aagcttttaa gaagtgccgg aatggcgtct acattaacac gacaaaaatt aaaagtctgt 20160

cgatgattaa aggcccacaa cgtgccgatt tgaatggcgt agttgtggag aaagttggag 20220

attctgatgt ggaattttgg tttgctgtgc gtaaagacgg tgacgatgtt atcttcagcc 20280

gtacagggag ccttgaaccg agccattacc ggagcccaca aggtaatccg ggtggtaatc 20340

gcgtgggtga tctcagcggt aatgaagctc tagcgcgtgg cactatcttt actcaaagca 20400

gattattatc ttctttcaca cctcgatcag agatggagaa agattttatg gatttagatg 20460

atgatgtgtt cattgcaaaa tatagtttac aggactacgc gtttgaacac gttgtttatg 20520

gtagttttaa ccagaagatt attggaggtt tgcatttgct tattggctta gcccgtaggc 20580

agcaaaaatc caatctggta attcaagagt tcgtgacata cgactctagc attcattcgt 20640

actttatcac tgacgagaac agtggtagta gtaagagtgt gtgcactgtt attgatttat 20700

tgttagatga ttttgtggac attgtaaagt ccctgaatct aaagtgtgtg agtaaggttg 20760

ttaatgttaa tgtggatttt aaggacttcc agtttatgtt gtggtgcaat gaggagaagg 20820

tcatgacttt ctatcctcgt ttgcaggctg ctgctgactg gaaacctggt tatgttatgc 20880

ctgtcttata taagtatttg gaatcgcctc tggaaagagt aaacctctgg aattatggca 20940

agccgattac tttacctaca ggatgtatga tgaatgttgc taagtatact caattatgtc 21000

aatatttgag cactacaaca ttagcagttc cggctaatat gcgtgtctta caccttggtg 21060

ccggttcgga taagggtgtt gcccctgggt ctgcagttct taggcagtgg ctaccagcgg 21120

gaagtattct tgtagataat gatgtgaatc catttgtgag tgacagtgtc gcctcatatt 21180

atggaaattg tataacctta ccctttgatt gtcagtggga tctgataatt tctgatatgt 21240

acgaccctct tactaagaac attggggagt acaacgtgag taaagatgga ttctttactt 21300

acctctgtca tttaattcgt gacaagttgg ctctgggtgg cagtgttgcc ataaaaataa 21360

cagagttttc ttggaacgct gagttatata gtttaatggg gaagtttgcg ttctggacaa 21420

tcttttgcac caacgtaaac gcctcttcaa gtgaaggatt tttgattggc ataaattggt 21480

tgaataagac ccgtaccgaa attgacggta aaaccatgca tgccaattat ctgttttgga 21540

gaaatagtac aatgtggaat ggaggggctt acagtctctt tgacatgagt aagttccctt 21600

tgaaagcggc tggtacggct gttgttagcc ttaaaccaga ccaaataaat gacttagtcc 21660

tctccttgat tgagaagggc aagttattag tgcgtgatac acgcaaagaa gtttttgttg 21720

gcgatagcct agtaaatgtc aaataa 21746

<210> 16

<211> 9589

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX_SYNCoat56

<400> 16

atctatactt gtcgtggctg tgaaaatggc ctttgctgac aagcctaatc atttcataaa 60

ctttcccctg gcccaattta gtggctttat gggtaagtat ttaaagctac agtctcaact 120

tgtggaaatg ggtttagact gtaaattaca gaaggcacca catgttagta ttaccctgct 180

tgatattaaa gcagaccaat acaaacaggt ggaatttgca atacaagaaa taatagatga 240

tctggcggca tatgagggag atattgtctt tgacaaccct cacatgcttg gcagatgcct 300

tgttcttgat gttagaggat ttgaagagtt gcatgaagat attgttgaaa ttctccgcag 360

aaggggttgc acggcagatc aatccagaca ctggattccg cactgcactg tggcccaatt 420

tgacgaagaa agagaaacaa aaggaatgca attctatcat aaagaaccct tctacctcaa 480

gcataacaac ctattaacgg atgctgggct tgagctcgtg aagataggtt cttccaaaat 540

agatgggttt tattgtagtg aactgagtgt ttggtgtggt gagaggcttt gttataagcc 600

tccaacaccc aaattcagtg atatatttgg ctattgctgc atagataaaa tacgtggtga 660

tttagaaata ggcgacctgc cgcaggatga tgaggaagcg tgggccgagc taagttacca 720

ctatcaaaga aacacctact tcttcagaca tgtgcacgat aatagcatct attttcgtac 780

cgtgtgtaga atgaagggtt gtatgtgttg atttgttttt acactattag tgtaataagc 840

ttattatttt gttgaaaagg gcaggatgtg catagctatg gctcctcgca cactgctttt 900

gctgatttga tgtcagctgg tgtttgggtt caatgaacct cttaacatcg tttcacattt 960

aaatgatgac tggtttctat ttggtgacag tcggtccgac tgtacctatg tagaaaataa 1020

cggtcatcct aaattagatt ggcttgacct cgacccaaag ttgtgtaatt caggaaagat 1080

ttccgcaaag agtggtaact ctctctttag gagttttcac ttcactgatt tttacaatta 1140

tacgggtgag ggataccaaa ttgtatttta tgaaggagtt aattttagtc ccagccatgg 1200

ctttaaatgc ctggctcatg gagataataa aagatggatg ggcaataaag ctcgatttta 1260

tgcccgagtg tatgagaaga tggcccaata taggagccta tcgtttgtta atgtgtctta 1320

tgcctatgga ggtaatgcaa agcccgcctc catttgcaaa gacaatactt taacactcaa 1380

taaccccacc ttcatatcga aggagtctaa ttatgttgat tactactacg agagtgaggc 1440

taatttcaca ctagaaggtt gtgatgaatt tatagtaccg ctctgtggtt ttaatggcca 1500

ttccaagggc tcgtcgtcgg atgctgccaa taaatattat actgactctc agagttacta 1560

taatatggat attggtgtct tatatgggtt caattcgacc ttggatgttg gcaacactgc 1620

taaggatccg ggtcttgatc tcacttgtag gtatcttgca ttgactcctg gtaattataa 1680

ggctgtgtcc ttagaatatt tgttaagctt accctcaaag gctatttgcc tccataagac 1740

aaagcgcttt atgcctgtgc aggtagttga ctcaaggtgg agtagcatcc gccagtcaga 1800

caatatgacc gctgcagcct gtcagctgcc atattgtttc tttcgcaaca catctgcgaa 1860

ttatagtggt ggcacacatg atgcgcacca tggtgatttt catttcaggc agttattgtc 1920

tggtttgtta tataatgttt cctgtattgc ccagcagggt gcatttcttt ataataatgt 1980

gtcgtcctct tggccagcct atgggtacgg tcattgtcca acggcagcta acattggtta 2040

tatggcacct gtttgtatct atgaccctct cccggtcata ctgctaggtg tgttattggg 2100

tatagctgtg ttgactattg tgtttctgat gttttatttt atgacggata gcggtgttag 2160

attgcatgag gcataatcta aacatgctgt tcgtgtttat tctatttttg ccctcttgtt 2220

tagggtatat tggtgatttt agatgtatcc agcttgtgaa ttcaaacggt gctaatgtta 2280

gtgctccaag cattagcacc gagacggttg aagtttcaca aggcctgggg acatattatg 2340

tgttagatcg agtttattta aatgccacat tattgcttac tggttactac ccggtcgatg 2400

gttctaagtt tagaaacctc gctcttacgg gaactaactc agttagcttg tcgtggtttc 2460

aaccacccta tttaagtcag tttaatgatg gcatatttgc gaaggtgcag aaccttaaga 2520

caagtacgcc atcaggtgca actgcatatt ttcctactat agttataggt agtttgtttg 2580

gctatacttc ctataccgtt gtaatagagc catataatgg tgttataatg gcctcagtgt 2640

gccagtatac catttgtcag ttaccttaca ctgattgtaa gcctaacact aatggtaata 2700

aactgatagg gttttggcac acggatgtaa aacccccaat ttgtgtgtta aagcgaaatt 2760

tcacgcttaa tgttaatgct gatgcatttt attttcattt ctaccaacat ggtggtactt 2820

tttatgcgta ctatgcggat aaaccctccg ctactacgtt tttgtttagt gtatatatcg 2880

gcgatatttt aacacagtat tatgtgttac ctttcatctg caacccaaca gctggtagca 2940

cttttgctcc gcgctattgg gttacacctt tggttaagcg ccaatatttg tttaatttca 3000

accagaaggg tgtcattact agtgctgttg attgtgctag tagttatacc agtgaaataa 3060

aatgtaagac ccagagcatg ttacctagca ctggtgtcta tgagttatcc ggttatacgg 3120

tccaaccagt tggagttgta taccggcgtg ttgctaacct cccagcttgt aatatagagg 3180

agtggcttac tgctaggtca gtcccctccc ctctcaactg ggagcgtaag acttttcaga 3240

attgcaattt taacttaagc agcctgttac gttatgttca ggctgagagt ttgttttgta 3300

ataatatcga tgcttccaaa gtgtatggcc gctgctttgg tagtatttca gttgataagt 3360

ttgctgtacc ccgaagtagg caagttgatt tacagcttgg taactctgga tttctgcaga 3420

ctgctaatta taagattgat acagctgcca cttcgtgtca gctgcattac accttgccta 3480

agaataatgt caccataaac aaccataacc cctcgtcttg gaataggagg tatggcttta 3540

atgatgctgg cgtctttggc aaaaaccaac atgacgttgt ttacgctcag caatgtttta 3600

ctgtaagatc tagttattgc ccgtgtgctc aaccggacat agttagccct tgcactactc 3660

agactaagcc taagtctgct tttgttaatg tgggtgacca ttgtgaaggc ttaggtgttt 3720

tagaagataa ttgtggcaat gctgatccac ataagggttg tatctgtgcc aacaattcat 3780

ttattggatg gtcacatgat acctgccttg ttaatgatcg ctgccaaatt tttgctaata 3840

tattgctgaa tggcattaat agtggtacca catgttccac agatttgcag ttgcctaata 3900

ctgaagtggt tactggcatt tgtgtcaaat atgacctcta cggtattact ggacaaggtg 3960

tttttaaaga ggttaaggct gactattata atagctggca aacccttctg tatgatgtta 4020

atggtaattt gaatggtttt cgtgatctta ccactaacaa gacttatacg ataaggagct 4080

gttatagtgg ccgtgtttct gctgcatttc ataaagatgc acccgaaccg gctctgctct 4140

atcgtaatat aaattgtagc tatgttttta gcaataatat ctcccgtgag gagaacccac 4200

ttaattactt tgatagttat ctgggttgtg ttgttaatgc tgataaccgc acggatgagg 4260

cgcttcctaa ttgtgatctc cgtatgggtg ctggcttatg cgttgattat tcaaaatcac 4320

gcagggctca ccgatcagtt tctactggct atcggttaac tacatttgag ccatacactc 4380

cgatgttagt taatgatagt gtccaatccg ttgatggatt atatgagatg caaataccaa 4440

ccaattttac tattgggcac catgaggagt tcattcaaac tagatctcca aaggtgacta 4500

tagattgtgc tgcatttgtc tgtggtgata acactgcatg caggcagcag ttggttgagt 4560

atggctcttt ctgtgttaat gttaatgcca ttcttaatga ggttaataac ctcttggata 4620

atatgcaact acaagttgct agtgcattaa tgcagggtgt tactataagc tcgagactgc 4680

cagacggcat ctcaggccct atagatgaca ttaattttag tcctctactt ggatgcatag 4740

gttcaacatg tgccgaggac ggcaatggac ctagtgcaat ccgagggcgt tctgctatag 4800

aggatttgtt atttgacaag gtcaaattat ctgatgttgg ctttgtcgag gcttataata 4860

attgcaccgg tggtcaagaa gttcgtgacc tcctttgtgt acaatctttt aatggcatca 4920

aagtattacc tcctgtgttg tcagagagtc agatctctgg ctacacaacc ggtgctactg 4980

cggcagctat gttcccaccg tggtcagcag ctgccggtgt gccatttagt ttaagtgttc 5040

aatatagaat taatggttta ggtgtcacta tgaatgtgct tagtgagaac caaaagatga 5100

ttgctagtgc ttttaacaat gcgctgggtg ctatccagga tgggtttgat gcaaccaatt 5160

ctgctttagg taagatccag tccgttgtta atgcaaatgc tgaagcactc aataacttac 5220

taaatcaact ttctaacagg tttggtgcta ttagtgcttc tttacaagaa attctaactc 5280

ggcttgaggc tgtagaagca aaagcccaga tagatcgtct tattaatggc aggttaactg 5340

cacttaatgc gtatatatcc aagcaactta gtgatagtac gcttattaaa gttagtgctg 5400

ctcaggccat agaaaaggtc aatgagtgcg ttaagagcca aaccacgcgt attaatttct 5460

gtggcaatgg taatcatata ttatctcttg tccagaatgc gccttatggc ttatatttta 5520

tacacttcag ctatgtgcca atatccttta caaccgcaaa tgtgagtcct ggactttgca 5580

tttctggtga tagaggatta gcacctaaag ctggatattt tgttcaagat gatggagaat 5640

ggaagttcac aggcagttca tattactacc ctgaacccat tacagataaa aacagtgtca 5700

ttatgagtag ttgcgcagta aactacacaa aggcacctga agttttcttg aacacttcaa 5760

tacctaatcc acccgacttt aaggaggagt tagataaatg gtttaagaat cagacgtcta 5820

ttgcgcctga tttatctctc gatttcgaga agttaaatgt tactttgctg gacctgacgt 5880

atgagatgaa caggattcag gatgcaatta agaagttaaa tgagagctac atcaacctca 5940

aggaagttgg cacatatgaa atgtatgtga aatggccttg gtatgtttgg ttgctaattg 6000

gattagctgg tgtagctgtt tgtgtgttgt tattctttat atgttgctgc acaggttgtg 6060

gctcatgttg ttttaagaag tgtggaaatt gttgtgatga gtatggagga caccaggaca 6120

gtattgtgat acataatatt tcctctcatg aggattgact atcacagcct ctcctggaaa 6180

gacagaaaat ctaaacaatt tatagcattc tcattgctac ctggccccgt aagaggcagt 6240

catagctatg gccgtgttgg tcctaaggct acattggctg ctgtctttat tggtccattt 6300

attgtagcat gtatgctagg cattggccta gtttatttat tgcaattgca agttcaaatt 6360

tttcatgtta aggataccat acgtgtgact ggcaagccag ccactgtgtc ttatactaca 6420

agtacaccag taacaccgag cgcgacgacg ctcgatggta ctacgtatac tttaattaga 6480

cccactagct cttatacaag agtttatctt ggtactccaa gaggttttga ttatagtaca 6540

tttgggccta agaccctaga ttatgttact aatctaaacc tcatcttaat tctggtcgtc 6600

catatacttt taaggcattg tccaggcata tgaggccaac agccacatgg atttggcatg 6660

tgagtgatgc atggttacgc cgcacgcggg actttggtgt cattcgccta gaagattttt 6720

gttttcaatt taattatagc caaccccgag ttggttattg tagagttcct ttaaaggctt 6780

ggtgtagcaa ccagggtaaa tttgcagcgc agtttaccct aaaaagttgc gaaaaaccag 6840

gtcacgaaaa atttattact agcttcacgg cctacggcag aactgtccaa caggccgtta 6900

gcaagttagt agaagaagct gttgatttta ttctttttag ggccacgcag ctcgaaagaa 6960

atgtttaatt tattccttac agacacagta tggtatgtgg ggcagattat ttttatattc 7020

gcagtgtgtt tgatggtcac cataattgtg gttgccttcc ttgcgtctat caaactttgt 7080

attcaacttt gcggtttatg taatactttg gtgctgtccc cttctattta tttgtatgat 7140

aggagtaagc agctttataa gtactataat gaagaaatga gactgcccct attagaggtg 7200

gatgatatct aatccaaaca ttatgagtag tactactcag gccccagagc ccgtctatca 7260

atggaccgcc gacgaggcag ttcaattcct taaggaatgg aacttctcgt tgggcattat 7320

actactcttt attactatca tactacagtt cggttacacg agccgtagca tgtttattta 7380

tgttgtgaaa atgataatct tgtggttaat gtggccactg actattgttt tgtgtatttt 7440

caattgcgtg tatgcgctaa ataatgtgta tcttggattt tctatagtgt ttactatagt 7500

gtccattgta atctggatca tgtattttgt gaacagcata aggttgttta tcaggactgg 7560

tagctggtgg agcttcaacc ccgaaacaaa caaccttatg tgtatagata tgaaaggtac 7620

cgtgtatgtt agacccatta ttgaggatta ccatacacta acagccacta ttattcgtgg 7680

ccacctctac atgcaaggtg ttaagctagg caccggtttc tctttgtctg acttgcccgc 7740

ttatgttaca gttgctaagg tgtcacacct ttgcacttat aagcgcgcat tcttagacaa 7800

ggtagacggt gttagcggtt ttgctgttta tgtgaagtcc aaggtcggaa attaccgact 7860

gccctcaaac aaaccgagtg gcgcggacac cgcattgttg agaacctaat ctaaacttta 7920

aggatgtctt ttgttcctgg gcaagaaaat gccggtggca gaagctcctc tgtaaaccgc 7980

gctggtaatg gaatcctcaa gaaaaccact tgggctgacc aaaccgagcg tggaccaaat 8040

aatcaaaata gaggcagaag gaatcagcca aagcagactg caactactca acccaactcc 8100

gggagtgtgg ttccccatta ctcctggttt tctggcatta cccagttcca aaagggaaag 8160

gagtttcagt ttgcagaagg acaaggagtg cctattgcca atggaatccc cgcttcagag 8220

caaaagggat attggtatag acacaaccgc cgttctttta aaacacctga tgggcagcag 8280

aagcaattac tgcccagatg gtatttttac tatcttggca cagggcccca tgctggagcc 8340

agttatggag acagcattga aggcgtcttt tgggttgcaa acagccaagc ggacaccaat 8400

acccgctctg atattgtcga aagggaccca agcagtcatg aggctattcc tactaggttt 8460

gcgcccggca cggtattgcc tcagggcttt tatgttgaag gctctggaag gtctgccccg 8520

gccagccgat ctggttcgcg gtcacaatcc cgtgggccaa ataatcgcgc tagaagcagt 8580

tccaaccagc gccagcctgc ctctactgta aaacctgata tggccgaaga aattgctgct 8640

cttgttttgg ctaagctcgg taaagatgcc ggccagccca agcaagtaac gaagcaaagt 8700

gccaaagaag tcaggcagaa aattttaaac aagcctcgcc aaaagaggac tccaaacaag 8760

cagtgcccag tgcagcagtg ttttggaaag agaggcccca atcagaattt tggaggctct 8820

gaaatgttaa aacttggaac tagtgatcca cagttcccca ttcttgcaga gttggctcca 8880

acagttggtg ccttcttctt tggatctaaa ttagaattgg tcaaaaagaa ttctggtggt 8940

gctgatgaac ccaccaaaga tgtgtatgag ctgcaatatt caggtgcagt tagatttgat 9000

agtactctac ctggttttga gactatcatg aaagtgttga atgagaattt gaatgcctac 9060

cagaaggatg gtggtgcaga tgtggtgagc ccaaagcccc aaagaaaagg gcgtagacag 9120

gctcaggaaa agaaagatga agtagataat gtaagcgttg caaagcccaa aagctctgtg 9180

cagcgaaatg taagtagaga attaacccca gaggatagaa gtctgttggc tcagatcctt 9240

gatgatggcg tagtgccaga tgggttagaa gatgactcta atgtgtaaag agaatgaatc 9300

ctatgtcggc gctcggtggt aacccctcgc gagaaagtcg ggataggaca ctctctatca 9360

gaatggatgt cttgctgtca taacagatag agaaggttgt ggcagaccct gtatcaatta 9420

gttgaaagag attgcaaaat agagaatgtg tgagagaagt tagcaaggtc ctacgtctaa 9480

ccataagaac ggcgataggc gccccctggg aacagctcac atcagggtac tattcctgca 9540

atgccctagt aaatgaatga agttgatcat ggccaattgg aagaatcac 9589

<210> 17

<211> 3822

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX-S19-1

<400> 17

atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc 60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac 120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc 180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat 240

aaccctgtcc taccatttaa tgatggtgtt tactttgctt ccactgagaa gtctaacata 300

ataagaggct ggatttttgg tactacttta gattcgaaaa cccagtccct acttattgtt 360

aataacgcta ctaatgttgt tatcaaagtc tgtgaatttc aattttgtaa cgatccattt 420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat 480

tctagtgcga ataattgcac ttttgaatac gtctctcagc cttttcttat ggaccttgaa 540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt tcaagaatat tgatggttac 600

ttcaagatat actctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt 660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact 720

ttacttgctt tacatagaag ttatttaact cctggtgatt cttcttcagg ttggacagct 780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctact gaagtacaat 840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag 900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc 960

caaccaacag aatctattgt tagatttcct aacatcacaa acttgtgccc ttttggtgaa 1020

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac 1080

tgtgttgctg attattctgt cctgtataat tccgcatcat tttccacttt taagtgttat 1140

ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt 1200

gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat 1260

tataactaca aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat 1320

cttgattcta aggttggtgg taattataat tacctgtaca gattgtttag gaagtctaat 1380

ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt 1440

aatggtgttg aaggttttaa ttgttacttt cctctgcaat catatggttt ccaacccact 1500

aatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca 1560

ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaagaacaa gtgtgtcaat 1620

ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg 1680

cctttccaac aatttggcag agacattgct gacactactg atgctgttcg tgatccacaa 1740

acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca 1800

ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc 1860

cctgttgcta ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct 1920

aatgtttttc aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat 1980

gagtgtgaca tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct 2040

cctcggagag caagaagtgt agctagtcaa tccatcattg cctacactat gtcacttggt 2100

gcagaaaatt cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt 2160

agcgttacca cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg 2220

tacatttgtg gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt 2280

acacaattaa accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa 2340

gtttttgcac aagtcaaaca aatttacaag acaccaccaa ttaaagattt tggcggtttt 2400

aattttagcc agatactgcc agatccatca aaaccaagca agaggtcatt tattgaagat 2460

ctactgttca acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc 2520

cttggtgata ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt 2580

ttgccacctt tgctcacaga tgaaatgatt gctcaataca cttctgcact gttagcaggt 2640

acaatcactt ctggttggac ttttggtgca ggtgctgcat tacaaatacc atttgctatg 2700

caaatggctt ataggtttaa tggtattgga gttacacaga atgttctcta tgagaaccaa 2760

aaattgattg ccaaccaatt taatagtgct attggcaaaa ttcaagactc actttcttcc 2820

acagcaagtg cacttggaaa acttcaagat gtggtcaacc aaaatgcaca agctttaaac 2880

acgcttgtta aacaacttag ctccaatttt ggtgcaattt caagtgtttt aaacgacatc 2940

ctttcacgtc ttgacaaagt tgaggctgaa gtgcaaattg ataggttgat cacaggcaga 3000

cttcaaagtt tgcagacata tgtgactcaa caattaatta gagctgcaga aatcagagct 3060

tctgctaatc ttgctgctac taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt 3120

gacttttgcg gaaagggcta tcatcttatg tcatttcctc agtcagcacc tcatggtgtc 3180

gtctttttgc atgtgactta tgtccctgca caagaaaaga acttcacaac tgctcctgcc 3240

atttgtcatg atggaaaagc acactttcct cgtgaaggtg tctttgtttc aaatggcaca 3300

cactggtttg taacacaaag gaatttttat gaaccacaaa tcattactac agacaacaca 3360

tttgtgtctg gtaactgtga tgttgtaata ggaattgtca acaacacagt ttatgatcct 3420

ttgcaacctg aattagactc attcaaggag gagcttgata aatacttcaa gaaccatacc 3480

tcaccagatg ttgatttagg tgacatctct ggcattaatg cttcagttgt aaacattcag 3540

aaagaaatcg accgcctcaa tgaggttgcc aagaatttaa atgaatctct catcgatctc 3600

caagaacttg gaaagtatga gcagtatata aaatggccat ggtacatttg gctaggtttt 3660

atagctggct tgattgccat agtaatggtg acaattatgc tttgctgtat gaccagttgc 3720

tgtagttgtc tcaagggctg ttgttcttgt ggatcctgct gcaaatttga cgaggacgac 3780

tctgagccag tgctcaaagg agtcaaatta cattacacat aa 3822

<210> 18

<211> 1273

<212> PRT

<213> Artificial sequence

<220>

<223> S-protein _ Sars-CoV2

<400> 18

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val

1 5 10 15

Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe

20 25 30

Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu

35 40 45

His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp

50 55 60

Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp

65 70 75 80

Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu

85 90 95

Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser

100 105 110

Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile

115 120 125

Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr

130 135 140

Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr

145 150 155 160

Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu

165 170 175

Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe

180 185 190

Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr

195 200 205

Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu

210 215 220

Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr

225 230 235 240

Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser

245 250 255

Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro

260 265 270

Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala

275 280 285

Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys

290 295 300

Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val

305 310 315 320

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys

325 330 335

Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala

340 345 350

Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu

355 360 365

Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro

370 375 380

Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe

385 390 395 400

Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly

405 410 415

Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys

420 425 430

Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn

435 440 445

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe

450 455 460

Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys

465 470 475 480

Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly

485 490 495

Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val

500 505 510

Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys

515 520 525

Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn

530 535 540

Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu

545 550 555 560

Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val

565 570 575

Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe

580 585 590

Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val

595 600 605

Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile

610 615 620

His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser

625 630 635 640

Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val

645 650 655

Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala

660 665 670

Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala

675 680 685

Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser

690 695 700

Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile

705 710 715 720

Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val

725 730 735

Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu

740 745 750

Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr

755 760 765

Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln

770 775 780

Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe

785 790 795 800

Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser

805 810 815

Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly

820 825 830

Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp

835 840 845

Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu

850 855 860

Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly

865 870 875 880

Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile

885 890 895

Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr

900 905 910

Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn

915 920 925

Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala

930 935 940

Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn

945 950 955 960

Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val

965 970 975

Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln

980 985 990

Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val

995 1000 1005

Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu

1010 1015 1020

Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val

1025 1030 1035 1040

Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala

1045 1050 1055

Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu

1060 1065 1070

Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His

1075 1080 1085

Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val

1090 1095 1100

Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr

1105 1110 1115 1120

Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr

1125 1130 1135

Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu

1140 1145 1150

Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp

1155 1160 1165

Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp

1170 1175 1180

Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu

1185 1190 1195 1200

Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile

1205 1210 1215

Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile

1220 1225 1230

Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys

1235 1240 1245

Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val

1250 1255 1260

Leu Lys Gly Val Lys Leu His Tyr Thr

1265 1270

<210> 19

<211> 4486

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX-S19-2

<400> 19

acgaacttat ggatttgttt atgagaatct tcacaattgg aactgtaact ttgaagcaag 60

gtgaaatcaa ggatgctact ccttcagatt ttgttagagc tactgcaacg ataccgatac 120

aagcatcact tcctttcgga tggcttattg ttggcgttgc acttcttgct gtttttcaga 180

gcgcttccaa aatcataacc ctcaaaaaga gatggcaact agcactctcc aagggtgttc 240

actttgtttg caacttgctg ttgttgtttg taacagttta ctcacatctt ttgcttgttg 300

ctgctggcct tgaagcccct tttctctatc tttatgcttt agtctacttc ttgcagagta 360

taaactttgt acgcataata atgaggcttt ggctttgctg gaaatgccgt tccaaaaacc 420

cattacttta tgatgccaac tattttcttt gctggcatac taattgttac gactattgta 480

taccttacaa tagtgtaact tcttcaattg tcattacttc aggtgatggc acaacaagtc 540

ctatttctga acatgactac cagattggtg gttatactga aaaatgggaa tctggagtaa 600

aagactgtgt tgtattacac agttacttca cttcagacta ttaccagctg tactcaactc 660

aattgagtac agacactggt gttgaacatg ttaccttctt catctacaat aaaatcgttg 720

atgagcctga agaacatgtc caaattcaca caatcgacgg ttcatccgga gttgttaatc 780

cagtaatgga accaatttat gatgaaccga cgacgactac tagcgtgcct ttgtaagcac 840

aagctgatga gtacgaactt atgtactcat tcgtttcgga agagacaggt acgttaatag 900

ttaatagcgt acttcttttt cttgctttcg tggtattctt gctagttaca ctagccattc 960

ttactgcgct tcgattgtgt gcgtactgtt gcaatattgt taacgtgagt cttgtaaaac 1020

cttcttttta cgtttactct cgtgttaaaa atctgaattc ttctcgggtt cctgatcttc 1080

tggtctaaac gaactaaata ttatattagt ttttctgttt ggaactttaa ttttagccat 1140

ggcagattcc aacggtacta ttaccgttga ggagctgaaa aagctccttg aacaatggaa 1200

cctagtaata ggtttcctat tccttacatg gatttgcctg ctgcaatttg cctatgccaa 1260

caggaatagg tttttgtaca tcattaagtt gattttcctc tggctgttat ggccagtaac 1320

tttagcttgt tttgtgcttg ctgctgttta cagaataaat tggatcaccg gtggaattgc 1380

tattgcaatg gcttgtcttg taggattgat gtggctaagc tacttcattg cttctttcag 1440

actgtttgcg cgtacgcgtt ccatgtggtc attcaatcca gaaactaaca ttcttctcaa 1500

cgtgccactc catggaacta ttctgactag accgcttcta gaaagtgaac tcgtaatcgg 1560

agctgttatc cttcgtggac atcttcgtat tgctggacat catctaggac gctgtgacat 1620

caaggatcta cctaaagaaa tcactgttgc tacatcacga acgctttctt attacaaatt 1680

gggagcttca cagcgtgtag caggtgattc aggttttgct gcatatagtc gctacaggat 1740

tggcaactat aaattaaaca cagaccattc cagtagcagt gacaatattg ctttgcttgt 1800

acagtaagtg acaacagatg tttcatctcg ttgactttca ggttactata gcagagatat 1860

tactaatcat catgaggact tttaaagttt ccatttggaa tcttgattac atcataaacc 1920

tcataattaa gaacttaagc aagtcactaa ctgagaataa atattctcaa ctagacgagg 1980

agcagccaat ggagattgat taaacgaaca tgaaaattat tcttttcttg gcactgataa 2040

cactcgctac ttgtgagctt tatcactacc aagagtgtgt tagaggtaca acagtacttt 2100

taaaagaacc ttgctcgtcg ggaacatacg agggcaattc accatttcat cctctagctg 2160

ataacaaatt tgcactgact tgctttagca ctcaatttgc ttttgcttgt cctgacggcg 2220

taaaacacgt ctatcagtta cgtgccagat cagtttcacc taaactgttc atcagacaag 2280

aggaagttca agaactttac tctccaattt ttcttattgt tgcggcaata gtgtttataa 2340

cactttgctt cacactcaaa agaaagacag aatgattgaa ctttcattaa ttgacttcta 2400

tttgtgcttt ttagcctttc tgctattcct tgttttaatt atgcttatta tcttttggtt 2460

ctcacttgaa ctgcaagatc ataatgaaac ttgtcacgcc taaacgaaca tgaaatttct 2520

tgttttctta ggaatcatca caactgtagc tgcatttcac caagaatgta gtttacagtc 2580

atgtactcaa catcaaccat atgtagttga tgacccgtgt cctattcact tctattctaa 2640

atggtatatc agagtaggag ctagaaaatc agcaccttta attgaattgt gcgtggatga 2700

ggctggttct aaatcaccca ttcagtacat cgatatcggt aattatacag tttcctgttt 2760

accttttaca attaactgcc aggaacctaa attgggtagt cttgtagtgc gttgttcgtt 2820

ctacgaggac tttttagagt atcatgacgt tcgtgttgtt ttagatttca tctaaacgaa 2880

caaactaaaa tgtctgataa tggacctcaa aatcagcgaa atgcacctcg cattacgttt 2940

ggtggaccat cagattcaac tggcagtaac cagaatggag aacgaagtgg tgcgcgatca 3000

aaacaacgcc gcccgcaagg tttacccaat aatactgcgt cttggttcac cgctctcact 3060

caacatggca aggaagattt aaaattccct cgaggacaag gcgttccaat taacaccaat 3120

agcagtccag atgaccaaat tggctactac cgccgcgcca caagacgaat tcgtggtggt 3180

gatggtaaaa tgaaagatct cagtccaaga tggtatttct actatctagg aactgggcca 3240

gaagctggac ttccttatgg tgctaacaaa gatggcatca tatgggttgc aactgaggga 3300

gccttgaata caccaaaaga tcacattggc accagaaatc ctgctaacaa tgctgcaatc 3360

gtgctacaac ttcctcaagg aacaacatta ccaaaaggtt tttacgcaga agggtctaga 3420

ggtggaagtc aagcctcttc tagatcatca tcacgtagtc gcaacagttc aagaaattca 3480

actccaggtt caagtagagg aacttctcct gctagaatgg ctggaaatgg aggtgatgct 3540

gctcttgctt tgttactact tgacagattg aaccagcttg agagcaaaat gtctggtaaa 3600

ggccaacaac aacaaggcca aactgtcact aagaaatctg ctgctgaggc ttctaagaag 3660

cctagacaaa aacgtactgc cactaaagca tacaatgtaa cacaagcttt cggcagacgt 3720

ggtccagaac aaactcaagg aaattttggg gatcaggaac taatcagaca aggaactgat 3780

tacaaacatt ggccgcaaat tgcacaattt gctccttctg cttcagcgtt ctttggaatg 3840

tcgagaattg gaatggaagt cacaccttcg ggaacatggt tgacctatac aggtgccatc 3900

aaattggatg acaaagatcc aaatttcaaa gatcaagtca ttttgctgaa taagcatatt 3960

gacgcataca aaacattccc accaacagag cctaaaaagg acaaaaagaa gaaggctgat 4020

gaaactcaag ccttaccgca gagacagaag aaacagcaaa ctgtgactct tcttcctgct 4080

gcagatttgg atgatttctc caaacaattg caacaatcca tgagcagtgc tgactcaact 4140

caggcctaaa ctcatgcaga ccacacaagg cagatgggct atataaacgt tttcgctttt 4200

ccgtttacga tatatagtct actcttgtgc agaatgaatt ctcgtaacta catagcacaa 4260

gtagatgtag ttaactttaa tctcacatag caatctttaa tcagtgtgta acattaggga 4320

ggacttgaaa gagccaccac attttcaccg aggccacgcg gagtacgatc gagtgtacag 4380

tgaacaatgc tagggagagc tgcctatatg gatgagccct aatgtgtaaa attaatttta 4440

gtagtgctat ccccatgtga ttttaatagc ttcttaggag aatgac 4486

<210> 20

<211> 275

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ ORF3a _ protein

<400> 20

Met Asp Leu Phe Met Arg Ile Phe Thr Ile Gly Thr Val Thr Leu Lys

1 5 10 15

Gln Gly Glu Ile Lys Asp Ala Thr Pro Ser Asp Phe Val Arg Ala Thr

20 25 30

Ala Thr Ile Pro Ile Gln Ala Ser Leu Pro Phe Gly Trp Leu Ile Val

35 40 45

Gly Val Ala Leu Leu Ala Val Phe Gln Ser Ala Ser Lys Ile Ile Thr

50 55 60

Leu Lys Lys Arg Trp Gln Leu Ala Leu Ser Lys Gly Val His Phe Val

65 70 75 80

Cys Asn Leu Leu Leu Leu Phe Val Thr Val Tyr Ser His Leu Leu Leu

85 90 95

Val Ala Ala Gly Leu Glu Ala Pro Phe Leu Tyr Leu Tyr Ala Leu Val

100 105 110

Tyr Phe Leu Gln Ser Ile Asn Phe Val Arg Ile Ile Met Arg Leu Trp

115 120 125

Leu Cys Trp Lys Cys Arg Ser Lys Asn Pro Leu Leu Tyr Asp Ala Asn

130 135 140

Tyr Phe Leu Cys Trp His Thr Asn Cys Tyr Asp Tyr Cys Ile Pro Tyr

145 150 155 160

Asn Ser Val Thr Ser Ser Ile Val Ile Thr Ser Gly Asp Gly Thr Thr

165 170 175

Ser Pro Ile Ser Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys

180 185 190

Trp Glu Ser Gly Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr

195 200 205

Ser Asp Tyr Tyr Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly

210 215 220

Val Glu His Val Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro

225 230 235 240

Glu Glu His Val Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val

245 250 255

Asn Pro Val Met Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser

260 265 270

Val Pro Leu

275

<210> 21

<211> 75

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ Structure _ protein _ E

<400> 21

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser

1 5 10 15

Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala

20 25 30

Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn

35 40 45

Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn

50 55 60

Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val

65 70 75

<210> 22

<211> 222

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ membrane _ glycoprotein _ M

<400> 22

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu

1 5 10 15

Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile

20 25 30

Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile

35 40 45

Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys

50 55 60

Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile

65 70 75 80

Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe

85 90 95

Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe

100 105 110

Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile

115 120 125

Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile

130 135 140

Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp

145 150 155 160

Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu

165 170 175

Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly

180 185 190

Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr

195 200 205

Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln

210 215 220

<210> 23

<211> 61

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ ORF6_ protein

<400> 23

Met Phe His Leu Val Asp Phe Gln Val Thr Ile Ala Glu Ile Leu Leu

1 5 10 15

Ile Ile Met Arg Thr Phe Lys Val Ser Ile Trp Asn Leu Asp Tyr Ile

20 25 30

Ile Asn Leu Ile Ile Lys Asn Leu Ser Lys Ser Leu Thr Glu Asn Lys

35 40 45

Tyr Ser Gln Leu Asp Glu Glu Gln Pro Met Glu Ile Asp

50 55 60

<210> 24

<211> 121

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ ORF7a _ protein

<400> 24

Met Lys Ile Ile Leu Phe Leu Ala Leu Ile Thr Leu Ala Thr Cys Glu

1 5 10 15

Leu Tyr His Tyr Gln Glu Cys Val Arg Gly Thr Thr Val Leu Leu Lys

20 25 30

Glu Pro Cys Ser Ser Gly Thr Tyr Glu Gly Asn Ser Pro Phe His Pro

35 40 45

Leu Ala Asp Asn Lys Phe Ala Leu Thr Cys Phe Ser Thr Gln Phe Ala

50 55 60

Phe Ala Cys Pro Asp Gly Val Lys His Val Tyr Gln Leu Arg Ala Arg

65 70 75 80

Ser Val Ser Pro Lys Leu Phe Ile Arg Gln Glu Glu Val Gln Glu Leu

85 90 95

Tyr Ser Pro Ile Phe Leu Ile Val Ala Ala Ile Val Phe Ile Thr Leu

100 105 110

Cys Phe Thr Leu Lys Arg Lys Thr Glu

115 120

<210> 25

<211> 121

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ ORF8_ protein

<400> 25

Met Lys Phe Leu Val Phe Leu Gly Ile Ile Thr Thr Val Ala Ala Phe

1 5 10 15

His Gln Glu Cys Ser Leu Gln Ser Cys Thr Gln His Gln Pro Tyr Val

20 25 30

Val Asp Asp Pro Cys Pro Ile His Phe Tyr Ser Lys Trp Tyr Ile Arg

35 40 45

Val Gly Ala Arg Lys Ser Ala Pro Leu Ile Glu Leu Cys Val Asp Glu

50 55 60

Ala Gly Ser Lys Ser Pro Ile Gln Tyr Ile Asp Ile Gly Asn Tyr Thr

65 70 75 80

Val Ser Cys Leu Pro Phe Thr Ile Asn Cys Gln Glu Pro Lys Leu Gly

85 90 95

Ser Leu Val Val Arg Cys Ser Phe Tyr Glu Asp Phe Leu Glu Tyr His

100 105 110

Asp Val Arg Val Val Leu Asp Phe Ile

115 120

<210> 26

<211> 419

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic nucleocapsid phosphoprotein

<400> 26

Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr

1 5 10 15

Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg

20 25 30

Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn

35 40 45

Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu

50 55 60

Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro

65 70 75 80

Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly

85 90 95

Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr

100 105 110

Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp

115 120 125

Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp

130 135 140

His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln

145 150 155 160

Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser

165 170 175

Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn

180 185 190

Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala

195 200 205

Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu

210 215 220

Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln

225 230 235 240

Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys

245 250 255

Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln

260 265 270

Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp

275 280 285

Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile

290 295 300

Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile

305 310 315 320

Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala

325 330 335

Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu

340 345 350

Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro

355 360 365

Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln

370 375 380

Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu

385 390 395 400

Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser

405 410 415

Thr Gln Ala

<210> 27

<211> 38

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ ORF10_ protein

<400> 27

Met Gly Tyr Ile Asn Val Phe Ala Phe Pro Phe Thr Ile Tyr Ser Leu

1 5 10 15

Leu Leu Cys Arg Met Asn Ser Arg Asn Tyr Ile Ala Gln Val Asp Val

20 25 30

Val Asn Phe Asn Leu Thr

35

<210> 28

<211> 18

<212> DNA

<213> Artificial sequence

<220>

<223> T7_ promoter

<400> 28

taatacgact cactatag 18

<210> 29

<211> 26

<212> DNA

<213> Artificial sequence

<220>

<223> PolyA-element

<400> 29

aaaaaaaaaa aaaaaaaaaa cggccg 26

<210> 30

<211> 21536

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX-polyprotein coding sequence

<400> 30

atggcaaaga tgggcaaata cggcctgggc ttcaaatggg ccccagaatt tccatggatg 60

cttccgaacg catcggagaa gttgggtaac cctgagaggt cagaggagga tgggttttgc 120

ccctctgctg cgcaagaacc gaaagttaaa ggaaaaactt tggttaatca cgtgagggtg 180

aattgtagcc ggcttccagc tttggaatgc tgtgttcagt ctgccataat ccgtgatatt 240

tttgtagatg aggatcccca gaaggtggag gcctcaacta tgatggcatt gcagttcggt 300

agtgccgtct tggttaagcc atccaagcgc ttgtctattc aggcatggac taatttgggt 360

gtgcttccca aaacagctgc catggggttg ttcaagcgcg tctgcctgtg taacaccagg 420

gagtgctctt gtgacgccca cgtggccttt caccttttta cggtccaacc cgatggtgta 480

tgcctgggta atggccgttt tataggctgg ttcgttccag tcacagccat accggagtat 540

gcgaagcagt ggttgcaacc ctggtccatc cttcttcgta agggtggtaa caaagggtct 600

gtgacatccg gccacttccg ccgcgctgtt accatgcctg tgtatgactt taatgtagag 660

gatgcttgtg aggaggttca tcttaacccg aagggtaagt actcctgcaa ggcgtatgcc 720

ctgctgaagg gctatcgcgg tgttaagccc atcctgtttg tggaccagta tggttgcgac 780

tatactggat gtctcgccaa gggtcttgag gactatggcg atctcacctt gagtgagatg 840

aaggagttgt tccctgtgtg gcgtgactcc ttggatagtg aagtccttgt ggcttggcac 900

gttgatcgag atcctcgggc tgctatgcgt ctgcagactc ttgctactgt acgttgcatt 960

gattatgtgg gccaaccgac cgaggatgtg gtggatggag atgtggtagt gcgtgagcct 1020

gctcatcttc tcgcagccaa tgccattgtt aaaagactcc cccgtttggt ggagactatg 1080

ctgtatacgg attcgtccgt tacagaattc tgttataaaa ccaagctgtg tgaatgcggt 1140

tttatcacgc agtttggcta tgtggattgt tgtggtgaca cctgtgattt tcgtgggtgg 1200

gttgccggca atatgatgga tggctttcca tgtccagggt gtaccaaaaa ttatatgccc 1260

tgggaattgg aggcccagtc atcaggtgtt ataccagaag gaggtgttct attcactcag 1320

agcactgata cagtgaatcg tgagtccttt aagctctacg gtcatgctgt tgtgcctttt 1380

ggttctgctg tgtattggag cccttgccca ggtatgtggc ttccagtaat ttggtcgtcg 1440

gttaagtcat actctggttt gacttataca ggagtagttg gttgtaaggc aattgttcaa 1500

gagacagacg ctatatgtcg ttctctgtat atggattatg tccagcacaa gtgtggcaat 1560

ctcgagcaga gagctatcct tggattggac gatgtctatc atagacagtt gcttgtgaat 1620

aggggtgact atagtctcct ccttgagaat gtggatttgt ttgttaagcg gcgcgctgaa 1680

tttgcttgca aattcgccac ctgtggagat ggtcttgtac ccctcctact agatggttta 1740

gtgccccgca gttattattt gattaagagt ggtcaagctt tcacctctat gatggttaat 1800

tttagccatg aggtgactga catgtgtatg gacatggctt tattgttcat gcatgatgtt 1860

aaagtggcca ctaagtatgt taagaaggtt actggcaaac tggccgtgcg ctttaaagcg 1920

ttgggtgtag ccgttgtcag aaaaattact gaatggtttg atttagccgt ggacattgct 1980

gctagtgccg ctggatggct ttgctaccag ctggtaaatg gcttatttgc agtggccaat 2040

ggtgttataa cctttgtaca ggaggtgcct gagcttgtca agaattttgt tgacaagttc 2100

aaggcatttt tcaaggtttt gatcgactct atgtcggttt ctatcttgtc tggacttact 2160

gttgtcaaga ctgcctcaaa tagggtgtgt cttgctggca gtaaggttta tgaagttgtg 2220

cagaaatctt tgtctgcata tgttatgcct gtgggttgca gcgaagccac ttgtttggtg 2280

ggtgagattg aacctgcagt ttttgaagat gatgttgttg atgtggttaa agccccatta 2340

acatatcaag gctgttgtaa gccacccact tctttcgaga agatttgtat tgtggataaa 2400

ttgtatatgg ccaagtgtgg tgatcaattt taccctgtgg ttgttgataa cgacactgtt 2460

ggcgtgttag atcagtgctg gaggtttccc tgtgcgggca agaaagtcga gtttaacgac 2520

aagcccaaag tcaggaagat accctccacc cgtaagatta agatcacctt cgcactggat 2580

gcgacctttg atagtgttct ttcgaaggcg tgttcagagt ttgaagttga taaagatgtt 2640

acattggatg agctgcttga tgttgtgctt gacgcagttg agagtacgct cagcccttgt 2700

aaggagcatg atgtgatagg cacaaaagtt tgtgctttac ttgataggtt ggcaggagat 2760

tatgtctatc tttttgatga gggaggcgat gaagtgatcg ccccgaggat gtattgttcc 2820

ttttctgctc ctgatgacga ggactgcgtt gcagcggatg ttgtagatgc agatgaaaac 2880

caagatgatg atgccgagga ctcagcagtc cttgtcgctg atacccaaga agaggacggc 2940

gttgccaagg ggcaggttga ggcggattcg gaaatttgcg ttgcgcatac tggtagtcaa 3000

gaagaattgg ctgagcctga tgctgtcgga tctcaaactc ccatcgcctc tgctgaggaa 3060

accgaagtcg gagaggcaag cgacagggaa gggattgctg aggcgaaggc aactgtgtgt 3120

gctgatgctg tagatgcctg ccccgatcaa gtggaggcat ttgaaattga aaaggtcgag 3180

gactctatct tggatgagct tcaaactgaa cttaatgcgc cagcggacaa gacctatgag 3240

gatgtcttgg cattcgatgc cgtatgctca gaggcgttgt ctgcattcta tgctgtgccg 3300

agtgatgaga cgcactttaa agtgtgtgga ttctattcgc ctgctataga gcgcactaat 3360

tgttggctgc gttctacttt gatagtaatg cagagtctac ctttggaatt taaagacttg 3420

gagatgcaaa agctctggtt gtcttacaag gccggctatg accaatgctt tgtggacaaa 3480

ctagttaaga gcgtgcccaa gtctattatc cttccacaag gtggttatgt ggcagatttt 3540

gcctatttct ttctaagcca gtgtagcttt aaagcttatg ctaactggcg ttgtttagag 3600

tgtgacatgg agttaaagct tcaaggcttg gacgccatgt ttttctatgg ggacgttgtg 3660

tctcatatgt gcaagtgtgg taatagcatg accttgttgt ctgcagatat accctacact 3720

ttgcattttg gagtgcgaga tgataagttt tgcgcttttt acacgccaag aaaggtcttt 3780

agggctgctt gtgcggtaga tgttaatgat tgtcactcta tggctgtagt agagggcaag 3840

caaattgatg gtaaagtggt taccaaattt attggtgaca aatttgattt tatggtgggt 3900

tacgggatga catttagtat gtctcctttt gaactcgccc agttatatgg ttcatgtata 3960

acaccaaatg tttgttttgt taaaggagat gttataaagg ttgttcgctt agttaatgct 4020

gaagtcattg ttaaccctgc taatgggcgt atggctcatg gtgccggcgt cgccggcgcc 4080

atagctgaaa aggcgggcag tgcttttatt aaagaaacct ccgatatggt gaaggctcag 4140

ggcgtttgcc aggttggtga atgctatgaa tctgccggtg gtaagttatg taaaaaggtg 4200

cttaacattg tagggccaga tgcgcgaggg catggcaagc aatgctattc acttttagag 4260

cgtgcttatc agcatattaa taagtgtgac aatgttgtca ctactttaat ttcggctggt 4320

atatttagtg tgcctactga tgtctcccta acttacttac ttggtgtagt gacaaagaat 4380

gtcattcttg tcagtaacaa ccaggatgat tttgatgtga tagagaagtg tcaggtgacc 4440

tccgttgctg gtaccaaagc gctatcactt caattggcca aaaatttgtg ccgtgatgta 4500

aagtttgtga cgaatgcatg tagttcgctt tttagtgaat cttgctttgt ctcaagctat 4560

gatgtgttgc aggaagttga agcgctgcga catgatatac aattggatga tgatgctcgt 4620

gtctttgtgc aggctaatat ggactgtctg cccacagact ggcgtctcgt taacaaattt 4680

gatagtgttg atggtgttag aaccattaag tattttgaat gcccgggcgg gatttttgta 4740

tccagccagg gcaaaaagtt tggttatgtt cagaatggtt catttaagga ggcgagtgtt 4800

agccaaataa gggctttact cgctaataag gttgatgtct tgtgtactgt tgatggtgtt 4860

aacttccgct cctgctgcgt agcagagggt gaagtttttg gcaagacatt aggttcagtc 4920

ttttgtgatg gcataaatgt caccaaagtt aggtgtagtg ccatttacaa gggtaaggtt 4980

ttctttcagt acagtgattt gtccgaggca gatcttgtgg ctgttaaaga tgcctttggt 5040

tttgatgaac cacaactgct gaagtactac actatgcttg gcatgtgtaa gtggccagta 5100

gttgtttgtg gcaattattt tgctttcaag cagtcaaata ataattgcta catcaacgtg 5160

gcatgtttaa tgctgcaaca cttgagttta aagtttccta agtggcaatg gcaagaggct 5220

tggaacgagt tccgctctgg taaaccacta aggtttgtgt ccttggtatt agcaaagggc 5280

agctttaaat ttaatgaacc ttctgattct atcgatttta tgcgtgtggt gctacgtgaa 5340

gcagatttga gtggtgccac gtgcaatttg gaatttgttt gtaaatgtgg tgtgaagcaa 5400

gagcagcgca aaggtgttga cgctgttatg cattttggta cgttggataa aggtgatctt 5460

gtcaggggtt ataatatcgc atgtacgtgc ggtagtaaac ttgtgcattg cacccaattt 5520

aacgtaccat ttttaatttg ctccaacaca ccagagggta ggaaactgcc cgacgatgtt 5580

gttgcagcta atatttttac tggtggtagt gtgggccatt acacgcatgt gaaatgtaaa 5640

cccaagtacc agctttatga tgcttgtaat gttaataagg tttcggaggc taagggtaat 5700

tttaccgatt gcctctacct taaaaattta aagcaaacct tctcgtctgt gctgacgact 5760

ttttatttag atgacgtaaa gtgtgtggag tataagccag atttatcgca gtattactgt 5820

gagtctggta aatattatac aaaacccatt attaaggccc aatttagaac atttgagaag 5880

gttgatggtg tctataccaa ctttaaattg gtgggacata gtattgctga aaaactcaat 5940

gctaagctgg gatttgattg taattctccc tttgtggagt ataaaattac agagtggcca 6000

acagctactg gagatgtggt gttggctagt gatgatttgt atgtaagtcg gtacttaagc 6060

gggtgcatta cttttggtaa accggttgtc tggcttggcc atgaggaagc atcgctgaaa 6120

tctctcacat attttaatag acctagtgtc gtttgtgaaa ataaatttaa cgtgttgccc 6180

gttgatgtca gtgaacccac ggacaagggg cctgtgcctg ctgcagtcct tgttaccggc 6240

gtccctggag ctgatgcgtc agctggtgcc ggtattgcca aggagcaaaa agcctgtgct 6300

tctgctagtg tggaggatca ggttgttacg gaggttcgtc aagagccatc tgtttcagct 6360

gctgatgtca aagaggttaa attgaatggt gttaaaaagc ctgttaaggt ggaaggtagt 6420

gtggttgtta atgatcccac tagcgaaacc aaagttgtta aaagtttgtc tattgttgat 6480

gtctatgata tgttcctgac agggtgtaag tatgtggttt ggactgctaa tgagttgtct 6540

cgactagtaa attcaccgac tgttagggag tatgtgaagt ggggtatggg aaagattgta 6600

acacccgcta agttgttgtt gttaagagat gagaagcaag agttcgtagc gccaaaagta 6660

gtcaaggcga aagctattgc ctgctattgt gctgtgaagt ggtttctcct ctattgtttt 6720

agttggataa agtttaatac tgacaataag gttatataca ccacagaagt agcttcaaag 6780

cttactttca agttgtgctg tttggccttt aagaatgcct tacagacgtt taattggagc 6840

gttgtgtcta ggggcttttt cctagttgca acggtctttt tactctggtt taactttttg 6900

tatgctaatg ttattttgag tgacttctat ttgcctaata ttgggcctct ccctacgttt 6960

gtgggacaga tagttgcgtg gtttaagact acatttggtg tgtcaaccat ctgtgatttc 7020

taccaggtga cggatttggg ctatagaagt tcgttttgta atggaagtat ggtatgtgaa 7080

ctatgcttct caggttttga tatgctggac aactatgatg ctataaatgt tgttcaacac 7140

gttgtagata ggcgtttgtc ctttgactat attagcctat ttaaactggt agttgagctt 7200

gtaatcggct actctcttta tactgtgtgc ttctacccac tgtttgtcct tattggaatg 7260

cagttattga ccacatggtt gcctgaattc tttatgctgg agactatgca ttggagtgct 7320

cgtttgtttg tgtttgttgc caatatgctt ccagctttta cgttactgcg attttacatc 7380

gtggtgacag ctatgtataa ggtctattgt ctttgtagac atgttatgta tggatgtagt 7440

aagcctggtt gcttgttttg ttataagaga aaccgtagtg tccgtgttaa gtgtagcacc 7500

gttgttggtg gttcactacg ctattacgat gtaatggcta acggcggcac aggtttctgt 7560

acaaagcacc agtggaactg tcttaattgc aattcctgga aaccaggcaa tacattcata 7620

actcatgaag cagcggcgga cctctctaag gagttgaaac gccctgtgaa tccaacagat 7680

tctgcttatt actcggtcac agaggttaag caggttggtt gttccatgcg tttgttctac 7740

gagagagatg gacagcgtgt ttatgatgat gttaatgcta gtttgtttgt ggacatgaat 7800

ggtctgctgc attctaaagt taaaggtgtg cctgaaacgc atgttgtggt tgttgagaat 7860

gaagctgata aagctggttt tctcggcgcc gcagtgtttt atgcacaatc gctctacaga 7920

cctatgttga tggtggaaaa gaaattaata actaccgcca acactggttt gtctgttagt 7980

cgaactatgt ttgaccttta tgtagattca ttgctgaacg tcctcgacgt ggatcgcaag 8040

agtctaacaa gttttgtaaa tgctgcgcac aactctctaa aggagggtgt tcagcttgaa 8100

caagttatgg atacctttat tggctgtgcc cgacgtaagt gtgctataga ttctgatgtt 8160

gaaaccaagt ctattaccaa gtccgtcatg tcggcagtaa atgctggcgt tgattttacg 8220

gatgagagtt gtaataactt ggtgcctacc tatgttaaaa gtgacactat cgttgcagcc 8280

gatttgggtg ttcttattca gaataatgct aagcatgtac aggctaatgt tgctaaagcc 8340

gctaatgtgg cttgcatttg gtctgtggat gcttttaacc agctatctgc tgacttacag 8400

cataggctgc gaaaagcatg ttcaaaaact ggcttgaaga ttaagcttac ttataataag 8460

caggaggcaa atgttcctat tttaactaca ccgttctctc ttaaaggggg cgctgttttt 8520

agtagaatgt tacaatggtt gtttgttgct aatttgattt gtttcattgt gttgtgggcc 8580

cttatgccaa catatgcagt gcacaaatcg gatatgcagt tgcctttata tgccagtttt 8640

aaagttatag ataacggtgt gctaagggat gtgtctgtta ctgacgcatg cttcgcaaac 8700

aaatttaatc aattcgacca atggtatgag tctacttttg gtcttgctta ttaccgcaac 8760

tctaaggctt gtcctgttgt ggttgctgta atagatcaag acattggcca taccttattt 8820

aatgttccta ccacagtttt aagatatgga tttcatgtgt tgcattttat aacccatgca 8880

tttgctactg atagcgtgca gtgttacacg ccacatatgc aaatccccta tgataatttc 8940

tatgctagtg gttgcgtgtt gtcatccctc tgtactatgc ttgcgcatgc agatggaacc 9000

ccgcatcctt attgttatac agggggtgtt atgcataatg cctctctgta tagttctttg 9060

gctcctcatg tccgttataa cctggctagt tcaaatggtt atatacgttt tcccgaagtg 9120

gttagtgaag gcattgtgcg tgttgtgcgc actcgctcta tgacctactg cagggttggt 9180

ttatgtgagg aggccgagga gggtatctgc tttaatttta atcgttcatg ggtattgaac 9240

aacccgtatt atagggccat gcctggaact ttttgtggta ggaatgcttt tgatttaata 9300

catcaagttt taggaggatt agtgcggcct attgatttct ttgccttaac ggcgagttca 9360

gtggctggtg ctatccttgc aattattgtc gttttggctt tctattattt aatcaagctt 9420

aagcgtgcct ttggtgacta cactagtgtt gtggttatca atgtaattgt gtggtgtata 9480

aattttctga tgctttttgt gtttcaggtt tatcccacat tgtcttgttt atatgcttgt 9540

ttctacttct acaccacgct ttatttccct tcggagataa gtgttgttat gcatttgcaa 9600

tggcttgtca tgtatggtgc tattatgccc ttgtggtttt gcattattta cgtggcagtc 9660

gttgtttcaa accatgcatt gtggttgttc tcttactgcc gcaaaattgg taccgaggtt 9720

cgtagtgacg gcacatttga ggaaatggcc cttactacct ttatgattac taaagaatct 9780

tattgtaagt tgaaaaactc tgtttctgat gttgctttta acaggtactt gagtctttac 9840

aacaagtacc gttacttcag tggcaaaatg gatactgccg cttatagaga ggctgcctgt 9900

tcacaactgg caaaggcaat ggaaacattt aaccataata atggtaatga tgttctctat 9960

cagcctccaa ccgcctctgt tactacatca tttttacagt ctggtatagt gaagatggtg 10020

tcgcccacct ctaaagtgga gccttgtatt gttagtgtta cttatggtaa catgacactt 10080

aatgggttgt ggttggatga taaagtttat tgcccaagac atgttatctg ttcttcagct 10140

gacatgacag accctgatta tcctaatttg ctttgtagag tgacatcaag tgatttttgt 10200

gttatgtctg gtcgtatgag ccttactgta atgtcttatc aaatgcaggg ctgccaactt 10260

gttttgactg ttacactgca aaatcctaac acgcctaagt attccttcgg tgttgttaag 10320

cctggtgaga catttactgt actggctgca tacaatggca gacctcaagg agccttccat 10380

gttacgcttc gtagtagcca taccataaag ggctcctttc tatgtggatc ctgcggttct 10440

gtaggatatg ttttaactgg cgatagtgta cgatttgttt atatgcatca gctagagttg 10500

agtactggtt gtcataccgg tactgacttt agtgggaact tttatggtcc ctatagagat 10560

gcgcaagttg tacaattgcc tgttcaggat tatacgcaga ctgttaatgt tgtagcttgg 10620

ctttatgctg ctatttttaa cagatgcaac tggtttgtgc aaagtgatag ttgttccctg 10680

gaggagttta atgtttgggc tatgaccaat ggttttagct caatcaaagc cgatcttgtc 10740

ttggatgcgc ttgcttctat gacaggcgtt acagttgaac aggtgttggc cgctattaag 10800

aggctgcatt ctggattcca gggcaaacaa attttaggta gttgtgtgct tgaagatgag 10860

ctgacaccaa gtgatgttta tcaacaacta gctggtgtca agctacagtc aaagcgcaca 10920

agagttataa aaggtacatg ttgctggata ttggcttcaa cgtttttgtt ctgtagcatt 10980

atctcagcat ttgtaaaatg gactatgttt atgtatgtta ctacccatat gttgggagtg 11040

acattgtgtg cactttgttt tgtaagcttt gctatgttgt tgatcaagca taagcatttg 11100

tatttaacta tgtacatcat gcctgtgtta tgcacactgt tttacaccaa ctatttggtt 11160

gtgtacaaac agagttttag aggtctagct tatgcttggc tttcacactt tgtccctgct 11220

gtagattata catatatgga tgaagtttta tatggtgttg tgttgctagt agctatggtg 11280

tttgttacca tgcgtagcat aaaccacgac gtcttttcta ttatgttctt ggttggtaga 11340

cttgtcagcc tggtatccat gtggtatttt ggagccaatt tagaggaaga ggtactattg 11400

ttcctcacat ccctatttgg cacgtacaca tggactacta tgttgtcatt ggctaccgct 11460

aaggttattg ctaaatggtt ggctgtgaat gtcttgtact tcacagacgt accgcaaatt 11520

aaattagttc tgttgagcta cttgtgtatt ggttatgtgt gttgttgtta ttggggaatc 11580

ttgtcactcc ttaatagcat ttttaggatg ccattgggcg tctacaatta taaaatctcc 11640

gttcaggagt tacgttatat gaatgctaat ggcttgcgcc cacctagaaa tagttttgag 11700

gccctgatgc ttaattttaa gctgttggga attggtggtg tgccagtcat tgaagtatct 11760

caaattcaat caagattgac ggatgttaaa tgtgctaatg ttgtgttgct taattgcctc 11820

cagcacttgc atattgcatc taattctaag ttgtggcagt attgtagtac tttgcacaat 11880

gaaatactgg ctacatctga tttgagcgtg gccttcgata agttggctca actcttagtt 11940

gttttatttg ctaatccagc agcagtggat agcaagtgcc ttgcaagtat tgaagaagtg 12000

agcgatgatt acgttcgcga caatactgtc ttgcaagcct tacagagtga atttgttaat 12060

atggctagct tcgttgagta tgaacttgct aagaagaatc tagatgaggc taaggctagc 12120

ggctctgcca atcaacagca gattaagcag ctagagaagg cgtgtaatat tgctaagtca 12180

gcatatgagc gcgacagagc tgttgctcgt aagctggaac gtatggctga tttagctctt 12240

acaaacatgt ataaagaagc tagaattaat gataagaaga gtaaggtagt gtctgcattg 12300

caaaccatgc tctttagtat ggtgcgtaag ctagataacc aagctcttaa ttctatttta 12360

gacaacgcag ttaagggttg tgtacctttg aatgcaatac catcattgac ttcgaacact 12420

ctgactataa tagtgccaga taagcaggtt tttgatcagg ttgtggataa tgtgtatgtc 12480

acctatgctg ggaatgtatg gcatatacag tttattcaag atgctgatgg tgctgttaaa 12540

caattgaatg agatagatgt taattcaacc tggcctctag tcattgctgc aaataggcat 12600

aatgaagtgt ctactgttgt tttgcagaac aatgagttga tgcctcagaa gttgagaact 12660

caggttgtca atagtggctc agatatgaat tgtaatactc ctacccagtg ttactataat 12720

actactggca cgggtaagat tgtgtatgct atacttagtg actgtgacgg cctgaagtac 12780

actaagatag taaaagaaga tggaaattgt gttgttttgg aattggatcc tccctgtaag 12840

ttttctgttc aggatgtgaa gggccttaaa attaagtacc tttactttgt gaaggggtgt 12900

aatacactgg ctagaggctg ggttgtaggc accttatcct cgacagtgag attgcaggcg 12960

ggtacggcaa ctgagtatgc ctccaactct gcaatactgt cgctgtgtgc gttttctgta 13020

gatcctaaga aaacgtactt ggattatata aaacagggtg gagttcccgt tactaattgt 13080

gttaagatgt tatgtgacca tgctggcact ggtatggcca ttactattaa gccggaggca 13140

accactaatc aggattctta tggtggtgct tccgtttgta tatattgccg ctcgcgtgtt 13200

gaacatccag atgttgatgg attgtgcaaa ttacgcggca agtttgtcca agtgccctta 13260

ggcataaaag atcctgtgtc atatgtgttg acgcatgatg tttgtcaggt ttgtggcttt 13320

tggcgagatg gtagctgttc ctgtgtaggc acaggctccc agtttcagtc aaaagacacg 13380

aactttttaa acggattcgg ggtacaagtg taaatgcccg tcttgtaccc tgtgccagtg 13440

gcttggacac tgatgttcaa ttaagggcat ttgacatttg taatgctaat cgagctggca 13500

ttggtttgta ttataaagtg aattgctgcc gcttccagcg tgtagatgag gacggcaaca 13560

agttggataa gttctttgtt gttaaaagaa ctaatttaga agtgtataac aaggagaaag 13620

aatgctatga gttgacaaaa gaatgcggtg ttgtggctga acacgagttc ttcacatttg 13680

atgtggaggg aagtcgggta ccacacatag tccgtaaaga tctttcaaag tttactatgt 13740

tagatctttg ctatgcattg cgtcattttg accgcaatga ttgttcaact cttaaggaaa 13800

ttctccttac atatgctgag tgtgaagagt cctacttcca aaagaaggac tggtatgatt 13860

ttgttgagaa tcctgatata attaatgtgt acaagaagct tggtcctata tttaatagag 13920

ccctgcttaa cactgccaag tttgcagacg cattagtgga ggcaggctta gtaggtgttt 13980

taacacttga taatcaagat ttatatggtc aatggtatga ctttggagat tttgtcaaga 14040

cagtacctgg ttgtggtgtt gccgtggcag actcttatta ttcatatatg atgccaatgc 14100

tgactatgtg tcatgcgttg gatagtgagt tgtttgttaa tggtacttat agggagtttg 14160

accttgttca gtatgatttt actgatttca agctagagct gttcactaag tattttaagc 14220

attggagtat gacctaccac ccgaacacct gtgagtgcga ggatgacagg tgcattattc 14280

attgcgccaa ttttaatata cttttcagca tggtcttacc taagacctgt tttgggcctc 14340

ttgttaggca gatatttgtg gatggtgttc ctttcgttgt gtcgatcggt taccattata 14400

aagaattagg tgttgttatg aatatggatg tggatacaca tcgttatcgc ttgtctctta 14460

aggacttgct tttgtatgct gcagaccctg cccttcatgt ggcgtctgct agtgcactgc 14520

ttgatttgcg cacatgttgt tttagcgttg cagctattac aagtggcgta aaatttcaaa 14580

cagttaaacc tggaaatttt aatcaggatt tctacgagtt tattttgagt aaaggcctgc 14640

ttaaagaggg gagctccgtt gatttgaagc acttcttctt tacgcaggat ggtaatgctg 14700

ctattactga ttacaattac tacaagtata atctacccac catggtggat attaagcagt 14760

tgttgtttgt tttagaagtt gttaataagt acttcgagat ctatgagggt gggtgtatac 14820

ccgcaacaca ggtcattgtt aataattatg acaagagtgc tggctatcca tttaataaat 14880

ttggaaaggc caggctctat tatgaggcat tatcatttga ggagcaggat gaaatttatg 14940

cgtataccaa acgcaatgtc ctgccgaccc taactcaaat gaatcttaaa tatgctatta 15000

gtgctaagaa tagggcccgc accgttgctg gtgtctctat tctcagtact atgactggca 15060

gaatgtttca tcaaaagtgt ctaaagagta tagcagctac tcgcggtgtt cctgtagtta 15120

taggcaccac gaagttctat ggcggttggg atgatatgtt acgccgcctt attaaagatg 15180

ttgatagtcc tgtactcatg ggttgggact atcctaaatg tgatcgtgct atgccaaaca 15240

tactgcgtat tgttagtagt ttggtgctag cccgtaaaca tgattcgtgc tgttcgcata 15300

cggatagatt ctatcgtctt gcgaacgagt gcgcccaagt tttgagtgaa attgttatgt 15360

gtggtggttg ttattatgtt aaaccaggtg gcactagtag tggggatgca accactgctt 15420

ttgctaattc tgtgtttaac atttgtcaag ctgtttccgc caatgtatgc tcgcttatgg 15480

catgcaatgg acacaaaatt gaagatttga gtatacgcga gttacaaaag cgcctatact 15540

ctaatgtcta tcgtgcggac catgttgacc ccgcatttgt tagtgagtat tatgagtttt 15600

taaacaagca ttttagtatg atgattttga gtgatgatgg tgttgtgtgt tataattcag 15660

agtttgcgtc caagggttat attgctaata taagtgcctt tcaacaggta ttatattatc 15720

aaaacaacgt gtttatgtct gaggccaaat gttgggtaga aacagacatc gaaaagggac 15780

cgcatgaatt ttgttctcaa catacaatgc tagtcaagat ggatggtgat gaagtctacc 15840

ttccataccc tgatccttcg agaatcttag gagcaggctg ttttgttgat gatttactca 15900

agactgatag cgttctcttg atagagcgtt tcgtaagtct tgcaattgat gcttatcctt 15960

tagtatacca tgagaaccca gagtatcaaa atgtgttccg ggtatattta gaatacatca 16020

agaagctgta caatgatctc ggtaatcaga tcctggacag ctacagtgtt attttaagta 16080

cttgtgatgg tcaaaagttt actgacgaga cgttttacaa gaacatgtat ttaagaagtg 16140

cagtgctgca aagcgttggt gcctgcgttg tctgtagttc tcaaacatca ttacgttgtg 16200

gcagttgcat acgcaagcct ttgctgtgtt gcaaatgcgc ctatgatcat gttatgtcca 16260

ctgatcataa atatgtcctg agtgtgtcac catatgtgtg taattcaccg ggatgtgatg 16320

taaatgatgt taccaaattg tatttaggtg gtatgtcata ttattgtgag gaccataaac 16380

cacagtattc attcaaattg gtgatgaatg gtatggtttt tggtttatat aagcagtctt 16440

gtactggttc gccctacata gaggatttta ataaaatcgc tagttgcaaa tggacagaag 16500

tcgatgatta tgtgctagct aatgaatgca ccgaacgcct taaattgttt gccgcagaaa 16560

cgcagaaggc cacagaagag gcctttaagc aatgttatgc gtcagcaacg atccgtgaga 16620

tcgtgagcga tcgggagtta attttatctt gggaaattgg taaagtccgc ccgccactta 16680

ataaaaatta cgtgttcacc ggctaccatt ttactaataa tggtaagaca gttttaggtg 16740

agtatgtttt tgataagagt gagttgacta atggtgtgta ttatcgcgcc acaaccactt 16800

ataagttatc tgtaggtgat gtgttcattt taacatcaca cgcagtgtct agtttaagtg 16860

ctcctacatt agtaccgcag gagaattata ctagcattcg ttttgctagt gtttatagtg 16920

tgcctgagac gtttcagaat aatgtgccta attatcagca cattggaatg aagcgctatt 16980

gtactgtaca gggaccgcct ggtactggta agtcccatct agccattggg ctagctgttt 17040

attattgtac agcgcgcgtg gtgtataccg ctgctagcca tgctgcagtt gacgcgctgt 17100

gtgaaaaggc acataaattt ctcaacatca acgactgcac gcgtattgtt cctgcaaagg 17160

tgcgtgtaga ttgttatgat aaattcaagg tcaatgacac cactcgcaag tatgtgttta 17220

ctacaataaa tgcattacct gagttggtga ctgacattat tgtcgttgat gaagttagta 17280

tgcttaccaa ctatgagctg tctgttatta acagtcgtgt tagggctaag cattatgtgt 17340

atattggcga cccggcgcag ttacctgcac cacgtgtgct actgaataag ggaactctag 17400

aacctagata ttttaattcc gttaccaagc taatgtgttg tttgggtcca gatattttct 17460

tgggcacctg ttatagatgc cctaaggaga ttgtggatac ggtgtcagcc ttggtttata 17520

ataataagct gaaggctaaa aatgataata gctccatgtg ctttaaggtt tattataagg 17580

gccagactac acatgagagt tctagtgctg ttaatatgca gcaaatacat ttaatttcca 17640

agtttctgaa ggcaaacccc agttggagta acgccgtatt tattagtcct tataactcgc 17700

agaactatgt tgctaagaga gtcttgggat tacaaaccca gacagtagac tcagcgcagg 17760

gttctgaata tgattttgtt atctactcac agactgcgga aacagcgcat tctgtcaatg 17820

taaatagatt caatgttgct attacacgtg ctaagaaggg tattctctgt gtcatgagta 17880

gtatgcaatt atttgagtct cttaatttta ctacactgac gttggataag attaacaatc 17940

cacgattaca gtgtactaca aatttgttta aggattgtag caggagctat gtaggatatc 18000

acccagccca tgcaccatcc tttttggcag ttgatgacaa atataaggta ggcggtgatt 18060

tagccgtttg ccttaatgtt gctgattctg ctgtcactta ttcgcggctt atatcactca 18120

tgggattcaa gcttgacttg acccttgatg gttattgtaa gctgtttata actagagatg 18180

aagctatcaa acgtgttaga gcctgggttg gcttcgatgc agaaggtgcc catgcgatac 18240

gtgatagcat tgggacaaat ttcccattac aattaggctt ttcgactgga attgattttg 18300

ttgtcgaagc cactggaatg tttgctgaga gagatggtta tgtctttaaa aaggcagccg 18360

cacgagctcc tcctggcgaa caatttaaac accttatccc acttatgtca agagggcaga 18420

aatgggatgt ggttcgcatt agaatagtac aaatgttgtc agaccaccta gtggatttgg 18480

cagacagtgt tgtacttgtg acgtgggctg ccagctttga gctcacatgt ttgcgatatt 18540

tcgctaaagt tggaagagaa gttgtgtgta gtgtctgcac caagcgtgcg acatgtttta 18600

attctagaac tggatactat ggatgctggc gacatagtta ttcctgtgat tacctgtaca 18660

acccactaat agttgacatt caacagtggg gatatacagg atctttaact agcaatcatg 18720

atcctatttg cagcgtgcat aagggtgctc atgttgcatc atctgatgct atcatgaccc 18780

ggtgtctagc tgttcatgat tgcttttgta agtctgttaa ttggaattta gaatacccca 18840

ttatttcaaa tgaggtcagt gttaatacct cctgcaggtt attgcagcgc gtaatgttta 18900

gggctgcgat gctatgcaat aggtatgatg tgtgttatga cattggcaac cctaaaggtc 18960

ttgcctgtgt caaaggatat gattttaagt tctatgacgc ctcccctgtt gttaagtctg 19020

ttaaacagtt tgtttacaaa tacgaggcac ataaagatca atttttagat ggtttgtgta 19080

tgttttggaa ctgcaatgtg gataagtatc cagcgaatgc agttgtgtgt aggtttgaca 19140

cgcgtgtgtt gaacaaatta aatctccctg gctgtaatgg tggcagtttg tatgttaaca 19200

aacatgcatt ccacaccagt ccctttaccc gggctgcctt cgagaatttg aagcctatgc 19260

ctttctttta ttattcagat acgccctgtg tgtatatgga aggcatggaa tctaagcagg 19320

tcgattatgt cccattgaga agcgctacat gcatcacaag atgcaattta ggtggcgctg 19380

tttgtttaaa acatgctgag gagtatcgtg agtaccttga gtcttacaat acggcaacca 19440

cagcgggttt tactttttgg gtctataaga cttttgattt ttacaacctt tggaatactt 19500

ttactaggct ccaaagttta gaaaatgtag tgtataacct ggtcaacgct ggacactttg 19560

atggccgggc gggtgaactg ccttgtgctg ttataggtga gaaagtcatt gccaagattc 19620

aaaatgagga tgtcgtggtc tttaaaaata acacgccatt ccccactaat gtggctgtcg 19680

aattatttgc taagcgcagt attcggcccc accccgagct taagctcttt agaaatttga 19740

atattgacgt gtgctggagt cacgtccttt gggattatgc taaggatagt gtgttttgca 19800

gttcgacgta taaggtctgc aaatacacag atttacagtg cattgaaagc ttgaatgtac 19860

tttttgatgg tcgtgataat ggtgctcttg aagcttttaa gaagtgccgg aatggcgtct 19920

acattaacac gacaaaaatt aaaagtctgt cgatgattaa aggcccacaa cgtgccgatt 19980

tgaatggcgt agttgtggag aaagttggag attctgatgt ggaattttgg tttgctgtgc 20040

gtaaagacgg tgacgatgtt atcttcagcc gtacagggag ccttgaaccg agccattacc 20100

ggagcccaca aggtaatccg ggtggtaatc gcgtgggtga tctcagcggt aatgaagctc 20160

tagcgcgtgg cactatcttt actcaaagca gattattatc ttctttcaca cctcgatcag 20220

agatggagaa agattttatg gatttagatg atgatgtgtt cattgcaaaa tatagtttac 20280

aggactacgc gtttgaacac gttgtttatg gtagttttaa ccagaagatt attggaggtt 20340

tgcatttgct tattggctta gcccgtaggc agcaaaaatc caatctggta attcaagagt 20400

tcgtgacata cgactctagc attcattcgt actttatcac tgacgagaac agtggtagta 20460

gtaagagtgt gtgcactgtt attgatttat tgttagatga ttttgtggac attgtaaagt 20520

ccctgaatct aaagtgtgtg agtaaggttg ttaatgttaa tgtggatttt aaggacttcc 20580

agtttatgtt gtggtgcaat gaggagaagg tcatgacttt ctatcctcgt ttgcaggctg 20640

ctgctgactg gaaacctggt tatgttatgc ctgtcttata taagtatttg gaatcgcctc 20700

tggaaagagt aaacctctgg aattatggca agccgattac tttacctaca ggatgtatga 20760

tgaatgttgc taagtatact caattatgtc aatatttgag cactacaaca ttagcagttc 20820

cggctaatat gcgtgtctta caccttggtg ccggttcgga taagggtgtt gcccctgggt 20880

ctgcagttct taggcagtgg ctaccagcgg gaagtattct tgtagataat gatgtgaatc 20940

catttgtgag tgacagtgtc gcctcatatt atggaaattg tataacctta ccctttgatt 21000

gtcagtggga tctgataatt tctgatatgt acgaccctct tactaagaac attggggagt 21060

acaacgtgag taaagatgga ttctttactt acctctgtca tttaattcgt gacaagttgg 21120

ctctgggtgg cagtgttgcc ataaaaataa cagagttttc ttggaacgct gagttatata 21180

gtttaatggg gaagtttgcg ttctggacaa tcttttgcac caacgtaaac gcctcttcaa 21240

gtgaaggatt tttgattggc ataaattggt tgaataagac ccgtaccgaa attgacggta 21300

aaaccatgca tgccaattat ctgttttgga gaaatagtac aatgtggaat ggaggggctt 21360

acagtctctt tgacatgagt aagttccctt tgaaagcggc tggtacggct gttgttagcc 21420

ttaaaccaga ccaaataaat gacttagtcc tctccttgat tgagaagggc aagttattag 21480

tgcgtgatac acgcaaagaa gtttttgttg gcgatagcct agtaaatgtc aaataa 21536

<210> 31

<211> 4470

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ replicated _ multimeric protein _1a

<400> 31

Met Ala Lys Met Gly Lys Tyr Gly Leu Gly Phe Lys Trp Ala Pro Glu

1 5 10 15

Phe Pro Trp Met Leu Pro Asn Ala Ser Glu Lys Leu Gly Asn Pro Glu

20 25 30

Arg Ser Glu Glu Asp Gly Phe Cys Pro Ser Ala Ala Gln Glu Pro Lys

35 40 45

Val Lys Gly Lys Thr Leu Val Asn His Val Arg Val Asn Cys Ser Arg

50 55 60

Leu Pro Ala Leu Glu Cys Cys Val Gln Ser Ala Ile Ile Arg Asp Ile

65 70 75 80

Phe Val Asp Glu Asp Pro Gln Lys Val Glu Ala Ser Thr Met Met Ala

85 90 95

Leu Gln Phe Gly Ser Ala Val Leu Val Lys Pro Ser Lys Arg Leu Ser

100 105 110

Ile Gln Ala Trp Thr Asn Leu Gly Val Leu Pro Lys Thr Ala Ala Met

115 120 125

Gly Leu Phe Lys Arg Val Cys Leu Cys Asn Thr Arg Glu Cys Ser Cys

130 135 140

Asp Ala His Val Ala Phe His Leu Phe Thr Val Gln Pro Asp Gly Val

145 150 155 160

Cys Leu Gly Asn Gly Arg Phe Ile Gly Trp Phe Val Pro Val Thr Ala

165 170 175

Ile Pro Glu Tyr Ala Lys Gln Trp Leu Gln Pro Trp Ser Ile Leu Leu

180 185 190

Arg Lys Gly Gly Asn Lys Gly Ser Val Thr Ser Gly His Phe Arg Arg

195 200 205

Ala Val Thr Met Pro Val Tyr Asp Phe Asn Val Glu Asp Ala Cys Glu

210 215 220

Glu Val His Leu Asn Pro Lys Gly Lys Tyr Ser Cys Lys Ala Tyr Ala

225 230 235 240

Leu Leu Lys Gly Tyr Arg Gly Val Lys Pro Ile Leu Phe Val Asp Gln

245 250 255

Tyr Gly Cys Asp Tyr Thr Gly Cys Leu Ala Lys Gly Leu Glu Asp Tyr

260 265 270

Gly Asp Leu Thr Leu Ser Glu Met Lys Glu Leu Phe Pro Val Trp Arg

275 280 285

Asp Ser Leu Asp Ser Glu Val Leu Val Ala Trp His Val Asp Arg Asp

290 295 300

Pro Arg Ala Ala Met Arg Leu Gln Thr Leu Ala Thr Val Arg Cys Ile

305 310 315 320

Asp Tyr Val Gly Gln Pro Thr Glu Asp Val Val Asp Gly Asp Val Val

325 330 335

Val Arg Glu Pro Ala His Leu Leu Ala Ala Asn Ala Ile Val Lys Arg

340 345 350

Leu Pro Arg Leu Val Glu Thr Met Leu Tyr Thr Asp Ser Ser Val Thr

355 360 365

Glu Phe Cys Tyr Lys Thr Lys Leu Cys Glu Cys Gly Phe Ile Thr Gln

370 375 380

Phe Gly Tyr Val Asp Cys Cys Gly Asp Thr Cys Asp Phe Arg Gly Trp

385 390 395 400

Val Ala Gly Asn Met Met Asp Gly Phe Pro Cys Pro Gly Cys Thr Lys

405 410 415

Asn Tyr Met Pro Trp Glu Leu Glu Ala Gln Ser Ser Gly Val Ile Pro

420 425 430

Glu Gly Gly Val Leu Phe Thr Gln Ser Thr Asp Thr Val Asn Arg Glu

435 440 445

Ser Phe Lys Leu Tyr Gly His Ala Val Val Pro Phe Gly Ser Ala Val

450 455 460

Tyr Trp Ser Pro Cys Pro Gly Met Trp Leu Pro Val Ile Trp Ser Ser

465 470 475 480

Val Lys Ser Tyr Ser Gly Leu Thr Tyr Thr Gly Val Val Gly Cys Lys

485 490 495

Ala Ile Val Gln Glu Thr Asp Ala Ile Cys Arg Ser Leu Tyr Met Asp

500 505 510

Tyr Val Gln His Lys Cys Gly Asn Leu Glu Gln Arg Ala Ile Leu Gly

515 520 525

Leu Asp Asp Val Tyr His Arg Gln Leu Leu Val Asn Arg Gly Asp Tyr

530 535 540

Ser Leu Leu Leu Glu Asn Val Asp Leu Phe Val Lys Arg Arg Ala Glu

545 550 555 560

Phe Ala Cys Lys Phe Ala Thr Cys Gly Asp Gly Leu Val Pro Leu Leu

565 570 575

Leu Asp Gly Leu Val Pro Arg Ser Tyr Tyr Leu Ile Lys Ser Gly Gln

580 585 590

Ala Phe Thr Ser Met Met Val Asn Phe Ser His Glu Val Thr Asp Met

595 600 605

Cys Met Asp Met Ala Leu Leu Phe Met His Asp Val Lys Val Ala Thr

610 615 620

Lys Tyr Val Lys Lys Val Thr Gly Lys Leu Ala Val Arg Phe Lys Ala

625 630 635 640

Leu Gly Val Ala Val Val Arg Lys Ile Thr Glu Trp Phe Asp Leu Ala

645 650 655

Val Asp Ile Ala Ala Ser Ala Ala Gly Trp Leu Cys Tyr Gln Leu Val

660 665 670

Asn Gly Leu Phe Ala Val Ala Asn Gly Val Ile Thr Phe Val Gln Glu

675 680 685

Val Pro Glu Leu Val Lys Asn Phe Val Asp Lys Phe Lys Ala Phe Phe

690 695 700

Lys Val Leu Ile Asp Ser Met Ser Val Ser Ile Leu Ser Gly Leu Thr

705 710 715 720

Val Val Lys Thr Ala Ser Asn Arg Val Cys Leu Ala Gly Ser Lys Val

725 730 735

Tyr Glu Val Val Gln Lys Ser Leu Ser Ala Tyr Val Met Pro Val Gly

740 745 750

Cys Ser Glu Ala Thr Cys Leu Val Gly Glu Ile Glu Pro Ala Val Phe

755 760 765

Glu Asp Asp Val Val Asp Val Val Lys Ala Pro Leu Thr Tyr Gln Gly

770 775 780

Cys Cys Lys Pro Pro Thr Ser Phe Glu Lys Ile Cys Ile Val Asp Lys

785 790 795 800

Leu Tyr Met Ala Lys Cys Gly Asp Gln Phe Tyr Pro Val Val Val Asp

805 810 815

Asn Asp Thr Val Gly Val Leu Asp Gln Cys Trp Arg Phe Pro Cys Ala

820 825 830

Gly Lys Lys Val Glu Phe Asn Asp Lys Pro Lys Val Arg Lys Ile Pro

835 840 845

Ser Thr Arg Lys Ile Lys Ile Thr Phe Ala Leu Asp Ala Thr Phe Asp

850 855 860

Ser Val Leu Ser Lys Ala Cys Ser Glu Phe Glu Val Asp Lys Asp Val

865 870 875 880

Thr Leu Asp Glu Leu Leu Asp Val Val Leu Asp Ala Val Glu Ser Thr

885 890 895

Leu Ser Pro Cys Lys Glu His Asp Val Ile Gly Thr Lys Val Cys Ala

900 905 910

Leu Leu Asp Arg Leu Ala Gly Asp Tyr Val Tyr Leu Phe Asp Glu Gly

915 920 925

Gly Asp Glu Val Ile Ala Pro Arg Met Tyr Cys Ser Phe Ser Ala Pro

930 935 940

Asp Asp Glu Asp Cys Val Ala Ala Asp Val Val Asp Ala Asp Glu Asn

945 950 955 960

Gln Asp Asp Asp Ala Glu Asp Ser Ala Val Leu Val Ala Asp Thr Gln

965 970 975

Glu Glu Asp Gly Val Ala Lys Gly Gln Val Glu Ala Asp Ser Glu Ile

980 985 990

Cys Val Ala His Thr Gly Ser Gln Glu Glu Leu Ala Glu Pro Asp Ala

995 1000 1005

Val Gly Ser Gln Thr Pro Ile Ala Ser Ala Glu Glu Thr Glu Val Gly

1010 1015 1020

Glu Ala Ser Asp Arg Glu Gly Ile Ala Glu Ala Lys Ala Thr Val Cys

1025 1030 1035 1040

Ala Asp Ala Val Asp Ala Cys Pro Asp Gln Val Glu Ala Phe Glu Ile

1045 1050 1055

Glu Lys Val Glu Asp Ser Ile Leu Asp Glu Leu Gln Thr Glu Leu Asn

1060 1065 1070

Ala Pro Ala Asp Lys Thr Tyr Glu Asp Val Leu Ala Phe Asp Ala Val

1075 1080 1085

Cys Ser Glu Ala Leu Ser Ala Phe Tyr Ala Val Pro Ser Asp Glu Thr

1090 1095 1100

His Phe Lys Val Cys Gly Phe Tyr Ser Pro Ala Ile Glu Arg Thr Asn

1105 1110 1115 1120

Cys Trp Leu Arg Ser Thr Leu Ile Val Met Gln Ser Leu Pro Leu Glu

1125 1130 1135

Phe Lys Asp Leu Glu Met Gln Lys Leu Trp Leu Ser Tyr Lys Ala Gly

1140 1145 1150

Tyr Asp Gln Cys Phe Val Asp Lys Leu Val Lys Ser Val Pro Lys Ser

1155 1160 1165

Ile Ile Leu Pro Gln Gly Gly Tyr Val Ala Asp Phe Ala Tyr Phe Phe

1170 1175 1180

Leu Ser Gln Cys Ser Phe Lys Ala Tyr Ala Asn Trp Arg Cys Leu Glu

1185 1190 1195 1200

Cys Asp Met Glu Leu Lys Leu Gln Gly Leu Asp Ala Met Phe Phe Tyr

1205 1210 1215

Gly Asp Val Val Ser His Met Cys Lys Cys Gly Asn Ser Met Thr Leu

1220 1225 1230

Leu Ser Ala Asp Ile Pro Tyr Thr Leu His Phe Gly Val Arg Asp Asp

1235 1240 1245

Lys Phe Cys Ala Phe Tyr Thr Pro Arg Lys Val Phe Arg Ala Ala Cys

1250 1255 1260

Ala Val Asp Val Asn Asp Cys His Ser Met Ala Val Val Glu Gly Lys

1265 1270 1275 1280

Gln Ile Asp Gly Lys Val Val Thr Lys Phe Ile Gly Asp Lys Phe Asp

1285 1290 1295

Phe Met Val Gly Tyr Gly Met Thr Phe Ser Met Ser Pro Phe Glu Leu

1300 1305 1310

Ala Gln Leu Tyr Gly Ser Cys Ile Thr Pro Asn Val Cys Phe Val Lys

1315 1320 1325

Gly Asp Val Ile Lys Val Val Arg Leu Val Asn Ala Glu Val Ile Val

1330 1335 1340

Asn Pro Ala Asn Gly Arg Met Ala His Gly Ala Gly Val Ala Gly Ala

1345 1350 1355 1360

Ile Ala Glu Lys Ala Gly Ser Ala Phe Ile Lys Glu Thr Ser Asp Met

1365 1370 1375

Val Lys Ala Gln Gly Val Cys Gln Val Gly Glu Cys Tyr Glu Ser Ala

1380 1385 1390

Gly Gly Lys Leu Cys Lys Lys Val Leu Asn Ile Val Gly Pro Asp Ala

1395 1400 1405

Arg Gly His Gly Lys Gln Cys Tyr Ser Leu Leu Glu Arg Ala Tyr Gln

1410 1415 1420

His Ile Asn Lys Cys Asp Asn Val Val Thr Thr Leu Ile Ser Ala Gly

1425 1430 1435 1440

Ile Phe Ser Val Pro Thr Asp Val Ser Leu Thr Tyr Leu Leu Gly Val

1445 1450 1455

Val Thr Lys Asn Val Ile Leu Val Ser Asn Asn Gln Asp Asp Phe Asp

1460 1465 1470

Val Ile Glu Lys Cys Gln Val Thr Ser Val Ala Gly Thr Lys Ala Leu

1475 1480 1485

Ser Leu Gln Leu Ala Lys Asn Leu Cys Arg Asp Val Lys Phe Val Thr

1490 1495 1500

Asn Ala Cys Ser Ser Leu Phe Ser Glu Ser Cys Phe Val Ser Ser Tyr

1505 1510 1515 1520

Asp Val Leu Gln Glu Val Glu Ala Leu Arg His Asp Ile Gln Leu Asp

1525 1530 1535

Asp Asp Ala Arg Val Phe Val Gln Ala Asn Met Asp Cys Leu Pro Thr

1540 1545 1550

Asp Trp Arg Leu Val Asn Lys Phe Asp Ser Val Asp Gly Val Arg Thr

1555 1560 1565

Ile Lys Tyr Phe Glu Cys Pro Gly Gly Ile Phe Val Ser Ser Gln Gly

1570 1575 1580

Lys Lys Phe Gly Tyr Val Gln Asn Gly Ser Phe Lys Glu Ala Ser Val

1585 1590 1595 1600

Ser Gln Ile Arg Ala Leu Leu Ala Asn Lys Val Asp Val Leu Cys Thr

1605 1610 1615

Val Asp Gly Val Asn Phe Arg Ser Cys Cys Val Ala Glu Gly Glu Val

1620 1625 1630

Phe Gly Lys Thr Leu Gly Ser Val Phe Cys Asp Gly Ile Asn Val Thr

1635 1640 1645

Lys Val Arg Cys Ser Ala Ile Tyr Lys Gly Lys Val Phe Phe Gln Tyr

1650 1655 1660

Ser Asp Leu Ser Glu Ala Asp Leu Val Ala Val Lys Asp Ala Phe Gly

1665 1670 1675 1680

Phe Asp Glu Pro Gln Leu Leu Lys Tyr Tyr Thr Met Leu Gly Met Cys

1685 1690 1695

Lys Trp Pro Val Val Val Cys Gly Asn Tyr Phe Ala Phe Lys Gln Ser

1700 1705 1710

Asn Asn Asn Cys Tyr Ile Asn Val Ala Cys Leu Met Leu Gln His Leu

1715 1720 1725

Ser Leu Lys Phe Pro Lys Trp Gln Trp Gln Glu Ala Trp Asn Glu Phe

1730 1735 1740

Arg Ser Gly Lys Pro Leu Arg Phe Val Ser Leu Val Leu Ala Lys Gly

1745 1750 1755 1760

Ser Phe Lys Phe Asn Glu Pro Ser Asp Ser Ile Asp Phe Met Arg Val

1765 1770 1775

Val Leu Arg Glu Ala Asp Leu Ser Gly Ala Thr Cys Asn Leu Glu Phe

1780 1785 1790

Val Cys Lys Cys Gly Val Lys Gln Glu Gln Arg Lys Gly Val Asp Ala

1795 1800 1805

Val Met His Phe Gly Thr Leu Asp Lys Gly Asp Leu Val Arg Gly Tyr

1810 1815 1820

Asn Ile Ala Cys Thr Cys Gly Ser Lys Leu Val His Cys Thr Gln Phe

1825 1830 1835 1840

Asn Val Pro Phe Leu Ile Cys Ser Asn Thr Pro Glu Gly Arg Lys Leu

1845 1850 1855

Pro Asp Asp Val Val Ala Ala Asn Ile Phe Thr Gly Gly Ser Val Gly

1860 1865 1870

His Tyr Thr His Val Lys Cys Lys Pro Lys Tyr Gln Leu Tyr Asp Ala

1875 1880 1885

Cys Asn Val Asn Lys Val Ser Glu Ala Lys Gly Asn Phe Thr Asp Cys

1890 1895 1900

Leu Tyr Leu Lys Asn Leu Lys Gln Thr Phe Ser Ser Val Leu Thr Thr

1905 1910 1915 1920

Phe Tyr Leu Asp Asp Val Lys Cys Val Glu Tyr Lys Pro Asp Leu Ser

1925 1930 1935

Gln Tyr Tyr Cys Glu Ser Gly Lys Tyr Tyr Thr Lys Pro Ile Ile Lys

1940 1945 1950

Ala Gln Phe Arg Thr Phe Glu Lys Val Asp Gly Val Tyr Thr Asn Phe

1955 1960 1965

Lys Leu Val Gly His Ser Ile Ala Glu Lys Leu Asn Ala Lys Leu Gly

1970 1975 1980

Phe Asp Cys Asn Ser Pro Phe Val Glu Tyr Lys Ile Thr Glu Trp Pro

1985 1990 1995 2000

Thr Ala Thr Gly Asp Val Val Leu Ala Ser Asp Asp Leu Tyr Val Ser

2005 2010 2015

Arg Tyr Leu Ser Gly Cys Ile Thr Phe Gly Lys Pro Val Val Trp Leu

2020 2025 2030

Gly His Glu Glu Ala Ser Leu Lys Ser Leu Thr Tyr Phe Asn Arg Pro

2035 2040 2045

Ser Val Val Cys Glu Asn Lys Phe Asn Val Leu Pro Val Asp Val Ser

2050 2055 2060

Glu Pro Thr Asp Lys Gly Pro Val Pro Ala Ala Val Leu Val Thr Gly

2065 2070 2075 2080

Val Pro Gly Ala Asp Ala Ser Ala Gly Ala Gly Ile Ala Lys Glu Gln

2085 2090 2095

Lys Ala Cys Ala Ser Ala Ser Val Glu Asp Gln Val Val Thr Glu Val

2100 2105 2110

Arg Gln Glu Pro Ser Val Ser Ala Ala Asp Val Lys Glu Val Lys Leu

2115 2120 2125

Asn Gly Val Lys Lys Pro Val Lys Val Glu Gly Ser Val Val Val Asn

2130 2135 2140

Asp Pro Thr Ser Glu Thr Lys Val Val Lys Ser Leu Ser Ile Val Asp

2145 2150 2155 2160

Val Tyr Asp Met Phe Leu Thr Gly Cys Lys Tyr Val Val Trp Thr Ala

2165 2170 2175

Asn Glu Leu Ser Arg Leu Val Asn Ser Pro Thr Val Arg Glu Tyr Val

2180 2185 2190

Lys Trp Gly Met Gly Lys Ile Val Thr Pro Ala Lys Leu Leu Leu Leu

2195 2200 2205

Arg Asp Glu Lys Gln Glu Phe Val Ala Pro Lys Val Val Lys Ala Lys

2210 2215 2220

Ala Ile Ala Cys Tyr Cys Ala Val Lys Trp Phe Leu Leu Tyr Cys Phe

2225 2230 2235 2240

Ser Trp Ile Lys Phe Asn Thr Asp Asn Lys Val Ile Tyr Thr Thr Glu

2245 2250 2255

Val Ala Ser Lys Leu Thr Phe Lys Leu Cys Cys Leu Ala Phe Lys Asn

2260 2265 2270

Ala Leu Gln Thr Phe Asn Trp Ser Val Val Ser Arg Gly Phe Phe Leu

2275 2280 2285

Val Ala Thr Val Phe Leu Leu Trp Phe Asn Phe Leu Tyr Ala Asn Val

2290 2295 2300

Ile Leu Ser Asp Phe Tyr Leu Pro Asn Ile Gly Pro Leu Pro Thr Phe

2305 2310 2315 2320

Val Gly Gln Ile Val Ala Trp Phe Lys Thr Thr Phe Gly Val Ser Thr

2325 2330 2335

Ile Cys Asp Phe Tyr Gln Val Thr Asp Leu Gly Tyr Arg Ser Ser Phe

2340 2345 2350

Cys Asn Gly Ser Met Val Cys Glu Leu Cys Phe Ser Gly Phe Asp Met

2355 2360 2365

Leu Asp Asn Tyr Asp Ala Ile Asn Val Val Gln His Val Val Asp Arg

2370 2375 2380

Arg Leu Ser Phe Asp Tyr Ile Ser Leu Phe Lys Leu Val Val Glu Leu

2385 2390 2395 2400

Val Ile Gly Tyr Ser Leu Tyr Thr Val Cys Phe Tyr Pro Leu Phe Val

2405 2410 2415

Leu Ile Gly Met Gln Leu Leu Thr Thr Trp Leu Pro Glu Phe Phe Met

2420 2425 2430

Leu Glu Thr Met His Trp Ser Ala Arg Leu Phe Val Phe Val Ala Asn

2435 2440 2445

Met Leu Pro Ala Phe Thr Leu Leu Arg Phe Tyr Ile Val Val Thr Ala

2450 2455 2460

Met Tyr Lys Val Tyr Cys Leu Cys Arg His Val Met Tyr Gly Cys Ser

2465 2470 2475 2480

Lys Pro Gly Cys Leu Phe Cys Tyr Lys Arg Asn Arg Ser Val Arg Val

2485 2490 2495

Lys Cys Ser Thr Val Val Gly Gly Ser Leu Arg Tyr Tyr Asp Val Met

2500 2505 2510

Ala Asn Gly Gly Thr Gly Phe Cys Thr Lys His Gln Trp Asn Cys Leu

2515 2520 2525

Asn Cys Asn Ser Trp Lys Pro Gly Asn Thr Phe Ile Thr His Glu Ala

2530 2535 2540

Ala Ala Asp Leu Ser Lys Glu Leu Lys Arg Pro Val Asn Pro Thr Asp

2545 2550 2555 2560

Ser Ala Tyr Tyr Ser Val Thr Glu Val Lys Gln Val Gly Cys Ser Met

2565 2570 2575

Arg Leu Phe Tyr Glu Arg Asp Gly Gln Arg Val Tyr Asp Asp Val Asn

2580 2585 2590

Ala Ser Leu Phe Val Asp Met Asn Gly Leu Leu His Ser Lys Val Lys

2595 2600 2605

Gly Val Pro Glu Thr His Val Val Val Val Glu Asn Glu Ala Asp Lys

2610 2615 2620

Ala Gly Phe Leu Gly Ala Ala Val Phe Tyr Ala Gln Ser Leu Tyr Arg

2625 2630 2635 2640

Pro Met Leu Met Val Glu Lys Lys Leu Ile Thr Thr Ala Asn Thr Gly

2645 2650 2655

Leu Ser Val Ser Arg Thr Met Phe Asp Leu Tyr Val Asp Ser Leu Leu

2660 2665 2670

Asn Val Leu Asp Val Asp Arg Lys Ser Leu Thr Ser Phe Val Asn Ala

2675 2680 2685

Ala His Asn Ser Leu Lys Glu Gly Val Gln Leu Glu Gln Val Met Asp

2690 2695 2700

Thr Phe Ile Gly Cys Ala Arg Arg Lys Cys Ala Ile Asp Ser Asp Val

2705 2710 2715 2720

Glu Thr Lys Ser Ile Thr Lys Ser Val Met Ser Ala Val Asn Ala Gly

2725 2730 2735

Val Asp Phe Thr Asp Glu Ser Cys Asn Asn Leu Val Pro Thr Tyr Val

2740 2745 2750

Lys Ser Asp Thr Ile Val Ala Ala Asp Leu Gly Val Leu Ile Gln Asn

2755 2760 2765

Asn Ala Lys His Val Gln Ala Asn Val Ala Lys Ala Ala Asn Val Ala

2770 2775 2780

Cys Ile Trp Ser Val Asp Ala Phe Asn Gln Leu Ser Ala Asp Leu Gln

2785 2790 2795 2800

His Arg Leu Arg Lys Ala Cys Ser Lys Thr Gly Leu Lys Ile Lys Leu

2805 2810 2815

Thr Tyr Asn Lys Gln Glu Ala Asn Val Pro Ile Leu Thr Thr Pro Phe

2820 2825 2830

Ser Leu Lys Gly Gly Ala Val Phe Ser Arg Met Leu Gln Trp Leu Phe

2835 2840 2845

Val Ala Asn Leu Ile Cys Phe Ile Val Leu Trp Ala Leu Met Pro Thr

2850 2855 2860

Tyr Ala Val His Lys Ser Asp Met Gln Leu Pro Leu Tyr Ala Ser Phe

2865 2870 2875 2880

Lys Val Ile Asp Asn Gly Val Leu Arg Asp Val Ser Val Thr Asp Ala

2885 2890 2895

Cys Phe Ala Asn Lys Phe Asn Gln Phe Asp Gln Trp Tyr Glu Ser Thr

2900 2905 2910

Phe Gly Leu Ala Tyr Tyr Arg Asn Ser Lys Ala Cys Pro Val Val Val

2915 2920 2925

Ala Val Ile Asp Gln Asp Ile Gly His Thr Leu Phe Asn Val Pro Thr

2930 2935 2940

Thr Val Leu Arg Tyr Gly Phe His Val Leu His Phe Ile Thr His Ala

2945 2950 2955 2960

Phe Ala Thr Asp Ser Val Gln Cys Tyr Thr Pro His Met Gln Ile Pro

2965 2970 2975

Tyr Asp Asn Phe Tyr Ala Ser Gly Cys Val Leu Ser Ser Leu Cys Thr

2980 2985 2990

Met Leu Ala His Ala Asp Gly Thr Pro His Pro Tyr Cys Tyr Thr Gly

2995 3000 3005

Gly Val Met His Asn Ala Ser Leu Tyr Ser Ser Leu Ala Pro His Val

3010 3015 3020

Arg Tyr Asn Leu Ala Ser Ser Asn Gly Tyr Ile Arg Phe Pro Glu Val

3025 3030 3035 3040

Val Ser Glu Gly Ile Val Arg Val Val Arg Thr Arg Ser Met Thr Tyr

3045 3050 3055

Cys Arg Val Gly Leu Cys Glu Glu Ala Glu Glu Gly Ile Cys Phe Asn

3060 3065 3070

Phe Asn Arg Ser Trp Val Leu Asn Asn Pro Tyr Tyr Arg Ala Met Pro

3075 3080 3085

Gly Thr Phe Cys Gly Arg Asn Ala Phe Asp Leu Ile His Gln Val Leu

3090 3095 3100

Gly Gly Leu Val Arg Pro Ile Asp Phe Phe Ala Leu Thr Ala Ser Ser

3105 3110 3115 3120

Val Ala Gly Ala Ile Leu Ala Ile Ile Val Val Leu Ala Phe Tyr Tyr

3125 3130 3135

Leu Ile Lys Leu Lys Arg Ala Phe Gly Asp Tyr Thr Ser Val Val Val

3140 3145 3150

Ile Asn Val Ile Val Trp Cys Ile Asn Phe Leu Met Leu Phe Val Phe

3155 3160 3165

Gln Val Tyr Pro Thr Leu Ser Cys Leu Tyr Ala Cys Phe Tyr Phe Tyr

3170 3175 3180

Thr Thr Leu Tyr Phe Pro Ser Glu Ile Ser Val Val Met His Leu Gln

3185 3190 3195 3200

Trp Leu Val Met Tyr Gly Ala Ile Met Pro Leu Trp Phe Cys Ile Ile

3205 3210 3215

Tyr Val Ala Val Val Val Ser Asn His Ala Leu Trp Leu Phe Ser Tyr

3220 3225 3230

Cys Arg Lys Ile Gly Thr Glu Val Arg Ser Asp Gly Thr Phe Glu Glu

3235 3240 3245

Met Ala Leu Thr Thr Phe Met Ile Thr Lys Glu Ser Tyr Cys Lys Leu

3250 3255 3260

Lys Asn Ser Val Ser Asp Val Ala Phe Asn Arg Tyr Leu Ser Leu Tyr

3265 3270 3275 3280

Asn Lys Tyr Arg Tyr Phe Ser Gly Lys Met Asp Thr Ala Ala Tyr Arg

3285 3290 3295

Glu Ala Ala Cys Ser Gln Leu Ala Lys Ala Met Glu Thr Phe Asn His

3300 3305 3310

Asn Asn Gly Asn Asp Val Leu Tyr Gln Pro Pro Thr Ala Ser Val Thr

3315 3320 3325

Thr Ser Phe Leu Gln Ser Gly Ile Val Lys Met Val Ser Pro Thr Ser

3330 3335 3340

Lys Val Glu Pro Cys Ile Val Ser Val Thr Tyr Gly Asn Met Thr Leu

3345 3350 3355 3360

Asn Gly Leu Trp Leu Asp Asp Lys Val Tyr Cys Pro Arg His Val Ile

3365 3370 3375

Cys Ser Ser Ala Asp Met Thr Asp Pro Asp Tyr Pro Asn Leu Leu Cys

3380 3385 3390

Arg Val Thr Ser Ser Asp Phe Cys Val Met Ser Gly Arg Met Ser Leu

3395 3400 3405

Thr Val Met Ser Tyr Gln Met Gln Gly Cys Gln Leu Val Leu Thr Val

3410 3415 3420

Thr Leu Gln Asn Pro Asn Thr Pro Lys Tyr Ser Phe Gly Val Val Lys

3425 3430 3435 3440

Pro Gly Glu Thr Phe Thr Val Leu Ala Ala Tyr Asn Gly Arg Pro Gln

3445 3450 3455

Gly Ala Phe His Val Thr Leu Arg Ser Ser His Thr Ile Lys Gly Ser

3460 3465 3470

Phe Leu Cys Gly Ser Cys Gly Ser Val Gly Tyr Val Leu Thr Gly Asp

3475 3480 3485

Ser Val Arg Phe Val Tyr Met His Gln Leu Glu Leu Ser Thr Gly Cys

3490 3495 3500

His Thr Gly Thr Asp Phe Ser Gly Asn Phe Tyr Gly Pro Tyr Arg Asp

3505 3510 3515 3520

Ala Gln Val Val Gln Leu Pro Val Gln Asp Tyr Thr Gln Thr Val Asn

3525 3530 3535

Val Val Ala Trp Leu Tyr Ala Ala Ile Phe Asn Arg Cys Asn Trp Phe

3540 3545 3550

Val Gln Ser Asp Ser Cys Ser Leu Glu Glu Phe Asn Val Trp Ala Met

3555 3560 3565

Thr Asn Gly Phe Ser Ser Ile Lys Ala Asp Leu Val Leu Asp Ala Leu

3570 3575 3580

Ala Ser Met Thr Gly Val Thr Val Glu Gln Val Leu Ala Ala Ile Lys

3585 3590 3595 3600

Arg Leu His Ser Gly Phe Gln Gly Lys Gln Ile Leu Gly Ser Cys Val

3605 3610 3615

Leu Glu Asp Glu Leu Thr Pro Ser Asp Val Tyr Gln Gln Leu Ala Gly

3620 3625 3630

Val Lys Leu Gln Ser Lys Arg Thr Arg Val Ile Lys Gly Thr Cys Cys

3635 3640 3645

Trp Ile Leu Ala Ser Thr Phe Leu Phe Cys Ser Ile Ile Ser Ala Phe

3650 3655 3660

Val Lys Trp Thr Met Phe Met Tyr Val Thr Thr His Met Leu Gly Val

3665 3670 3675 3680

Thr Leu Cys Ala Leu Cys Phe Val Ser Phe Ala Met Leu Leu Ile Lys

3685 3690 3695

His Lys His Leu Tyr Leu Thr Met Tyr Ile Met Pro Val Leu Cys Thr

3700 3705 3710

Leu Phe Tyr Thr Asn Tyr Leu Val Val Tyr Lys Gln Ser Phe Arg Gly

3715 3720 3725

Leu Ala Tyr Ala Trp Leu Ser His Phe Val Pro Ala Val Asp Tyr Thr

3730 3735 3740

Tyr Met Asp Glu Val Leu Tyr Gly Val Val Leu Leu Val Ala Met Val

3745 3750 3755 3760

Phe Val Thr Met Arg Ser Ile Asn His Asp Val Phe Ser Ile Met Phe

3765 3770 3775

Leu Val Gly Arg Leu Val Ser Leu Val Ser Met Trp Tyr Phe Gly Ala

3780 3785 3790

Asn Leu Glu Glu Glu Val Leu Leu Phe Leu Thr Ser Leu Phe Gly Thr

3795 3800 3805

Tyr Thr Trp Thr Thr Met Leu Ser Leu Ala Thr Ala Lys Val Ile Ala

3810 3815 3820

Lys Trp Leu Ala Val Asn Val Leu Tyr Phe Thr Asp Val Pro Gln Ile

3825 3830 3835 3840

Lys Leu Val Leu Leu Ser Tyr Leu Cys Ile Gly Tyr Val Cys Cys Cys

3845 3850 3855

Tyr Trp Gly Ile Leu Ser Leu Leu Asn Ser Ile Phe Arg Met Pro Leu

3860 3865 3870

Gly Val Tyr Asn Tyr Lys Ile Ser Val Gln Glu Leu Arg Tyr Met Asn

3875 3880 3885

Ala Asn Gly Leu Arg Pro Pro Arg Asn Ser Phe Glu Ala Leu Met Leu

3890 3895 3900

Asn Phe Lys Leu Leu Gly Ile Gly Gly Val Pro Val Ile Glu Val Ser

3905 3910 3915 3920

Gln Ile Gln Ser Arg Leu Thr Asp Val Lys Cys Ala Asn Val Val Leu

3925 3930 3935

Leu Asn Cys Leu Gln His Leu His Ile Ala Ser Asn Ser Lys Leu Trp

3940 3945 3950

Gln Tyr Cys Ser Thr Leu His Asn Glu Ile Leu Ala Thr Ser Asp Leu

3955 3960 3965

Ser Val Ala Phe Asp Lys Leu Ala Gln Leu Leu Val Val Leu Phe Ala

3970 3975 3980

Asn Pro Ala Ala Val Asp Ser Lys Cys Leu Ala Ser Ile Glu Glu Val

3985 3990 3995 4000

Ser Asp Asp Tyr Val Arg Asp Asn Thr Val Leu Gln Ala Leu Gln Ser

4005 4010 4015

Glu Phe Val Asn Met Ala Ser Phe Val Glu Tyr Glu Leu Ala Lys Lys

4020 4025 4030

Asn Leu Asp Glu Ala Lys Ala Ser Gly Ser Ala Asn Gln Gln Gln Ile

4035 4040 4045

Lys Gln Leu Glu Lys Ala Cys Asn Ile Ala Lys Ser Ala Tyr Glu Arg

4050 4055 4060

Asp Arg Ala Val Ala Arg Lys Leu Glu Arg Met Ala Asp Leu Ala Leu

4065 4070 4075 4080

Thr Asn Met Tyr Lys Glu Ala Arg Ile Asn Asp Lys Lys Ser Lys Val

4085 4090 4095

Val Ser Ala Leu Gln Thr Met Leu Phe Ser Met Val Arg Lys Leu Asp

4100 4105 4110

Asn Gln Ala Leu Asn Ser Ile Leu Asp Asn Ala Val Lys Gly Cys Val

4115 4120 4125

Pro Leu Asn Ala Ile Pro Ser Leu Thr Ser Asn Thr Leu Thr Ile Ile

4130 4135 4140

Val Pro Asp Lys Gln Val Phe Asp Gln Val Val Asp Asn Val Tyr Val

4145 4150 4155 4160

Thr Tyr Ala Gly Asn Val Trp His Ile Gln Phe Ile Gln Asp Ala Asp

4165 4170 4175

Gly Ala Val Lys Gln Leu Asn Glu Ile Asp Val Asn Ser Thr Trp Pro

4180 4185 4190

Leu Val Ile Ala Ala Asn Arg His Asn Glu Val Ser Thr Val Val Leu

4195 4200 4205

Gln Asn Asn Glu Leu Met Pro Gln Lys Leu Arg Thr Gln Val Val Asn

4210 4215 4220

Ser Gly Ser Asp Met Asn Cys Asn Thr Pro Thr Gln Cys Tyr Tyr Asn

4225 4230 4235 4240

Thr Thr Gly Thr Gly Lys Ile Val Tyr Ala Ile Leu Ser Asp Cys Asp

4245 4250 4255

Gly Leu Lys Tyr Thr Lys Ile Val Lys Glu Asp Gly Asn Cys Val Val

4260 4265 4270

Leu Glu Leu Asp Pro Pro Cys Lys Phe Ser Val Gln Asp Val Lys Gly

4275 4280 4285

Leu Lys Ile Lys Tyr Leu Tyr Phe Val Lys Gly Cys Asn Thr Leu Ala

4290 4295 4300

Arg Gly Trp Val Val Gly Thr Leu Ser Ser Thr Val Arg Leu Gln Ala

4305 4310 4315 4320

Gly Thr Ala Thr Glu Tyr Ala Ser Asn Ser Ala Ile Leu Ser Leu Cys

4325 4330 4335

Ala Phe Ser Val Asp Pro Lys Lys Thr Tyr Leu Asp Tyr Ile Lys Gln

4340 4345 4350

Gly Gly Val Pro Val Thr Asn Cys Val Lys Met Leu Cys Asp His Ala

4355 4360 4365

Gly Thr Gly Met Ala Ile Thr Ile Lys Pro Glu Ala Thr Thr Asn Gln

4370 4375 4380

Asp Ser Tyr Gly Gly Ala Ser Val Cys Ile Tyr Cys Arg Ser Arg Val

4385 4390 4395 4400

Glu His Pro Asp Val Asp Gly Leu Cys Lys Leu Arg Gly Lys Phe Val

4405 4410 4415

Gln Val Pro Leu Gly Ile Lys Asp Pro Val Ser Tyr Val Leu Thr His

4420 4425 4430

Asp Val Cys Gln Val Cys Gly Phe Trp Arg Asp Gly Ser Cys Ser Cys

4435 4440 4445

Val Gly Thr Gly Ser Gln Phe Gln Ser Lys Asp Thr Asn Phe Leu Asn

4450 4455 4460

Gly Phe Gly Val Gln Val

4465 4470

<210> 32

<211> 2714

<212> PRT

<213> Artificial sequence

<220>

<223> synthetic _ replicated _ polyprotein 1ab

<400> 32

Arg Ile Arg Gly Thr Ser Val Asn Ala Arg Leu Val Pro Cys Ala Ser

1 5 10 15

Gly Leu Asp Thr Asp Val Gln Leu Arg Ala Phe Asp Ile Cys Asn Ala

20 25 30

Asn Arg Ala Gly Ile Gly Leu Tyr Tyr Lys Val Asn Cys Cys Arg Phe

35 40 45

Gln Arg Val Asp Glu Asp Gly Asn Lys Leu Asp Lys Phe Phe Val Val

50 55 60

Lys Arg Thr Asn Leu Glu Val Tyr Asn Lys Glu Lys Glu Cys Tyr Glu

65 70 75 80

Leu Thr Lys Glu Cys Gly Val Val Ala Glu His Glu Phe Phe Thr Phe

85 90 95

Asp Val Glu Gly Ser Arg Val Pro His Ile Val Arg Lys Asp Leu Ser

100 105 110

Lys Phe Thr Met Leu Asp Leu Cys Tyr Ala Leu Arg His Phe Asp Arg

115 120 125

Asn Asp Cys Ser Thr Leu Lys Glu Ile Leu Leu Thr Tyr Ala Glu Cys

130 135 140

Glu Glu Ser Tyr Phe Gln Lys Lys Asp Trp Tyr Asp Phe Val Glu Asn

145 150 155 160

Pro Asp Ile Ile Asn Val Tyr Lys Lys Leu Gly Pro Ile Phe Asn Arg

165 170 175

Ala Leu Leu Asn Thr Ala Lys Phe Ala Asp Ala Leu Val Glu Ala Gly

180 185 190

Leu Val Gly Val Leu Thr Leu Asp Asn Gln Asp Leu Tyr Gly Gln Trp

195 200 205

Tyr Asp Phe Gly Asp Phe Val Lys Thr Val Pro Gly Cys Gly Val Ala

210 215 220

Val Ala Asp Ser Tyr Tyr Ser Tyr Met Met Pro Met Leu Thr Met Cys

225 230 235 240

His Ala Leu Asp Ser Glu Leu Phe Val Asn Gly Thr Tyr Arg Glu Phe

245 250 255

Asp Leu Val Gln Tyr Asp Phe Thr Asp Phe Lys Leu Glu Leu Phe Thr

260 265 270

Lys Tyr Phe Lys His Trp Ser Met Thr Tyr His Pro Asn Thr Cys Glu

275 280 285

Cys Glu Asp Asp Arg Cys Ile Ile His Cys Ala Asn Phe Asn Ile Leu

290 295 300

Phe Ser Met Val Leu Pro Lys Thr Cys Phe Gly Pro Leu Val Arg Gln

305 310 315 320

Ile Phe Val Asp Gly Val Pro Phe Val Val Ser Ile Gly Tyr His Tyr

325 330 335

Lys Glu Leu Gly Val Val Met Asn Met Asp Val Asp Thr His Arg Tyr

340 345 350

Arg Leu Ser Leu Lys Asp Leu Leu Leu Tyr Ala Ala Asp Pro Ala Leu

355 360 365

His Val Ala Ser Ala Ser Ala Leu Leu Asp Leu Arg Thr Cys Cys Phe

370 375 380

Ser Val Ala Ala Ile Thr Ser Gly Val Lys Phe Gln Thr Val Lys Pro

385 390 395 400

Gly Asn Phe Asn Gln Asp Phe Tyr Glu Phe Ile Leu Ser Lys Gly Leu

405 410 415

Leu Lys Glu Gly Ser Ser Val Asp Leu Lys His Phe Phe Phe Thr Gln

420 425 430

Asp Gly Asn Ala Ala Ile Thr Asp Tyr Asn Tyr Tyr Lys Tyr Asn Leu

435 440 445

Pro Thr Met Val Asp Ile Lys Gln Leu Leu Phe Val Leu Glu Val Val

450 455 460

Asn Lys Tyr Phe Glu Ile Tyr Glu Gly Gly Cys Ile Pro Ala Thr Gln

465 470 475 480

Val Ile Val Asn Asn Tyr Asp Lys Ser Ala Gly Tyr Pro Phe Asn Lys

485 490 495

Phe Gly Lys Ala Arg Leu Tyr Tyr Glu Ala Leu Ser Phe Glu Glu Gln

500 505 510

Asp Glu Ile Tyr Ala Tyr Thr Lys Arg Asn Val Leu Pro Thr Leu Thr

515 520 525

Gln Met Asn Leu Lys Tyr Ala Ile Ser Ala Lys Asn Arg Ala Arg Thr

530 535 540

Val Ala Gly Val Ser Ile Leu Ser Thr Met Thr Gly Arg Met Phe His

545 550 555 560

Gln Lys Cys Leu Lys Ser Ile Ala Ala Thr Arg Gly Val Pro Val Val

565 570 575

Ile Gly Thr Thr Lys Phe Tyr Gly Gly Trp Asp Asp Met Leu Arg Arg

580 585 590

Leu Ile Lys Asp Val Asp Ser Pro Val Leu Met Gly Trp Asp Tyr Pro

595 600 605

Lys Cys Asp Arg Ala Met Pro Asn Ile Leu Arg Ile Val Ser Ser Leu

610 615 620

Val Leu Ala Arg Lys His Asp Ser Cys Cys Ser His Thr Asp Arg Phe

625 630 635 640

Tyr Arg Leu Ala Asn Glu Cys Ala Gln Val Leu Ser Glu Ile Val Met

645 650 655

Cys Gly Gly Cys Tyr Tyr Val Lys Pro Gly Gly Thr Ser Ser Gly Asp

660 665 670

Ala Thr Thr Ala Phe Ala Asn Ser Val Phe Asn Ile Cys Gln Ala Val

675 680 685

Ser Ala Asn Val Cys Ser Leu Met Ala Cys Asn Gly His Lys Ile Glu

690 695 700

Asp Leu Ser Ile Arg Glu Leu Gln Lys Arg Leu Tyr Ser Asn Val Tyr

705 710 715 720

Arg Ala Asp His Val Asp Pro Ala Phe Val Ser Glu Tyr Tyr Glu Phe

725 730 735

Leu Asn Lys His Phe Ser Met Met Ile Leu Ser Asp Asp Gly Val Val

740 745 750

Cys Tyr Asn Ser Glu Phe Ala Ser Lys Gly Tyr Ile Ala Asn Ile Ser

755 760 765

Ala Phe Gln Gln Val Leu Tyr Tyr Gln Asn Asn Val Phe Met Ser Glu

770 775 780

Ala Lys Cys Trp Val Glu Thr Asp Ile Glu Lys Gly Pro His Glu Phe

785 790 795 800

Cys Ser Gln His Thr Met Leu Val Lys Met Asp Gly Asp Glu Val Tyr

805 810 815

Leu Pro Tyr Pro Asp Pro Ser Arg Ile Leu Gly Ala Gly Cys Phe Val

820 825 830

Asp Asp Leu Leu Lys Thr Asp Ser Val Leu Leu Ile Glu Arg Phe Val

835 840 845

Ser Leu Ala Ile Asp Ala Tyr Pro Leu Val Tyr His Glu Asn Pro Glu

850 855 860

Tyr Gln Asn Val Phe Arg Val Tyr Leu Glu Tyr Ile Lys Lys Leu Tyr

865 870 875 880

Asn Asp Leu Gly Asn Gln Ile Leu Asp Ser Tyr Ser Val Ile Leu Ser

885 890 895

Thr Cys Asp Gly Gln Lys Phe Thr Asp Glu Thr Phe Tyr Lys Asn Met

900 905 910

Tyr Leu Arg Ser Ala Val Leu Gln Ser Val Gly Ala Cys Val Val Cys

915 920 925

Ser Ser Gln Thr Ser Leu Arg Cys Gly Ser Cys Ile Arg Lys Pro Leu

930 935 940

Leu Cys Cys Lys Cys Ala Tyr Asp His Val Met Ser Thr Asp His Lys

945 950 955 960

Tyr Val Leu Ser Val Ser Pro Tyr Val Cys Asn Ser Pro Gly Cys Asp

965 970 975

Val Asn Asp Val Thr Lys Leu Tyr Leu Gly Gly Met Ser Tyr Tyr Cys

980 985 990

Glu Asp His Lys Pro Gln Tyr Ser Phe Lys Leu Val Met Asn Gly Met

995 1000 1005

Val Phe Gly Leu Tyr Lys Gln Ser Cys Thr Gly Ser Pro Tyr Ile Glu

1010 1015 1020

Asp Phe Asn Lys Ile Ala Ser Cys Lys Trp Thr Glu Val Asp Asp Tyr

1025 1030 1035 1040

Val Leu Ala Asn Glu Cys Thr Glu Arg Leu Lys Leu Phe Ala Ala Glu

1045 1050 1055

Thr Gln Lys Ala Thr Glu Glu Ala Phe Lys Gln Cys Tyr Ala Ser Ala

1060 1065 1070

Thr Ile Arg Glu Ile Val Ser Asp Arg Glu Leu Ile Leu Ser Trp Glu

1075 1080 1085

Ile Gly Lys Val Arg Pro Pro Leu Asn Lys Asn Tyr Val Phe Thr Gly

1090 1095 1100

Tyr His Phe Thr Asn Asn Gly Lys Thr Val Leu Gly Glu Tyr Val Phe

1105 1110 1115 1120

Asp Lys Ser Glu Leu Thr Asn Gly Val Tyr Tyr Arg Ala Thr Thr Thr

1125 1130 1135

Tyr Lys Leu Ser Val Gly Asp Val Phe Ile Leu Thr Ser His Ala Val

1140 1145 1150

Ser Ser Leu Ser Ala Pro Thr Leu Val Pro Gln Glu Asn Tyr Thr Ser

1155 1160 1165

Ile Arg Phe Ala Ser Val Tyr Ser Val Pro Glu Thr Phe Gln Asn Asn

1170 1175 1180

Val Pro Asn Tyr Gln His Ile Gly Met Lys Arg Tyr Cys Thr Val Gln

1185 1190 1195 1200

Gly Pro Pro Gly Thr Gly Lys Ser His Leu Ala Ile Gly Leu Ala Val

1205 1210 1215

Tyr Tyr Cys Thr Ala Arg Val Val Tyr Thr Ala Ala Ser His Ala Ala

1220 1225 1230

Val Asp Ala Leu Cys Glu Lys Ala His Lys Phe Leu Asn Ile Asn Asp

1235 1240 1245

Cys Thr Arg Ile Val Pro Ala Lys Val Arg Val Asp Cys Tyr Asp Lys

1250 1255 1260

Phe Lys Val Asn Asp Thr Thr Arg Lys Tyr Val Phe Thr Thr Ile Asn

1265 1270 1275 1280

Ala Leu Pro Glu Leu Val Thr Asp Ile Ile Val Val Asp Glu Val Ser

1285 1290 1295

Met Leu Thr Asn Tyr Glu Leu Ser Val Ile Asn Ser Arg Val Arg Ala

1300 1305 1310

Lys His Tyr Val Tyr Ile Gly Asp Pro Ala Gln Leu Pro Ala Pro Arg

1315 1320 1325

Val Leu Leu Asn Lys Gly Thr Leu Glu Pro Arg Tyr Phe Asn Ser Val

1330 1335 1340

Thr Lys Leu Met Cys Cys Leu Gly Pro Asp Ile Phe Leu Gly Thr Cys

1345 1350 1355 1360

Tyr Arg Cys Pro Lys Glu Ile Val Asp Thr Val Ser Ala Leu Val Tyr

1365 1370 1375

Asn Asn Lys Leu Lys Ala Lys Asn Asp Asn Ser Ser Met Cys Phe Lys

1380 1385 1390

Val Tyr Tyr Lys Gly Gln Thr Thr His Glu Ser Ser Ser Ala Val Asn

1395 1400 1405

Met Gln Gln Ile His Leu Ile Ser Lys Phe Leu Lys Ala Asn Pro Ser

1410 1415 1420

Trp Ser Asn Ala Val Phe Ile Ser Pro Tyr Asn Ser Gln Asn Tyr Val

1425 1430 1435 1440

Ala Lys Arg Val Leu Gly Leu Gln Thr Gln Thr Val Asp Ser Ala Gln

1445 1450 1455

Gly Ser Glu Tyr Asp Phe Val Ile Tyr Ser Gln Thr Ala Glu Thr Ala

1460 1465 1470

His Ser Val Asn Val Asn Arg Phe Asn Val Ala Ile Thr Arg Ala Lys

1475 1480 1485

Lys Gly Ile Leu Cys Val Met Ser Ser Met Gln Leu Phe Glu Ser Leu

1490 1495 1500

Asn Phe Thr Thr Leu Thr Leu Asp Lys Ile Asn Asn Pro Arg Leu Gln

1505 1510 1515 1520

Cys Thr Thr Asn Leu Phe Lys Asp Cys Ser Arg Ser Tyr Val Gly Tyr

1525 1530 1535

His Pro Ala His Ala Pro Ser Phe Leu Ala Val Asp Asp Lys Tyr Lys

1540 1545 1550

Val Gly Gly Asp Leu Ala Val Cys Leu Asn Val Ala Asp Ser Ala Val

1555 1560 1565

Thr Tyr Ser Arg Leu Ile Ser Leu Met Gly Phe Lys Leu Asp Leu Thr

1570 1575 1580

Leu Asp Gly Tyr Cys Lys Leu Phe Ile Thr Arg Asp Glu Ala Ile Lys

1585 1590 1595 1600

Arg Val Arg Ala Trp Val Gly Phe Asp Ala Glu Gly Ala His Ala Ile

1605 1610 1615

Arg Asp Ser Ile Gly Thr Asn Phe Pro Leu Gln Leu Gly Phe Ser Thr

1620 1625 1630

Gly Ile Asp Phe Val Val Glu Ala Thr Gly Met Phe Ala Glu Arg Asp

1635 1640 1645

Gly Tyr Val Phe Lys Lys Ala Ala Ala Arg Ala Pro Pro Gly Glu Gln

1650 1655 1660

Phe Lys His Leu Ile Pro Leu Met Ser Arg Gly Gln Lys Trp Asp Val

1665 1670 1675 1680

Val Arg Ile Arg Ile Val Gln Met Leu Ser Asp His Leu Val Asp Leu

1685 1690 1695

Ala Asp Ser Val Val Leu Val Thr Trp Ala Ala Ser Phe Glu Leu Thr

1700 1705 1710

Cys Leu Arg Tyr Phe Ala Lys Val Gly Arg Glu Val Val Cys Ser Val

1715 1720 1725

Cys Thr Lys Arg Ala Thr Cys Phe Asn Ser Arg Thr Gly Tyr Tyr Gly

1730 1735 1740

Cys Trp Arg His Ser Tyr Ser Cys Asp Tyr Leu Tyr Asn Pro Leu Ile

1745 1750 1755 1760

Val Asp Ile Gln Gln Trp Gly Tyr Thr Gly Ser Leu Thr Ser Asn His

1765 1770 1775

Asp Pro Ile Cys Ser Val His Lys Gly Ala His Val Ala Ser Ser Asp

1780 1785 1790

Ala Ile Met Thr Arg Cys Leu Ala Val His Asp Cys Phe Cys Lys Ser

1795 1800 1805

Val Asn Trp Asn Leu Glu Tyr Pro Ile Ile Ser Asn Glu Val Ser Val

1810 1815 1820

Asn Thr Ser Cys Arg Leu Leu Gln Arg Val Met Phe Arg Ala Ala Met

1825 1830 1835 1840

Leu Cys Asn Arg Tyr Asp Val Cys Tyr Asp Ile Gly Asn Pro Lys Gly

1845 1850 1855

Leu Ala Cys Val Lys Gly Tyr Asp Phe Lys Phe Tyr Asp Ala Ser Pro

1860 1865 1870

Val Val Lys Ser Val Lys Gln Phe Val Tyr Lys Tyr Glu Ala His Lys

1875 1880 1885

Asp Gln Phe Leu Asp Gly Leu Cys Met Phe Trp Asn Cys Asn Val Asp

1890 1895 1900

Lys Tyr Pro Ala Asn Ala Val Val Cys Arg Phe Asp Thr Arg Val Leu

1905 1910 1915 1920

Asn Lys Leu Asn Leu Pro Gly Cys Asn Gly Gly Ser Leu Tyr Val Asn

1925 1930 1935

Lys His Ala Phe His Thr Ser Pro Phe Thr Arg Ala Ala Phe Glu Asn

1940 1945 1950

Leu Lys Pro Met Pro Phe Phe Tyr Tyr Ser Asp Thr Pro Cys Val Tyr

1955 1960 1965

Met Glu Gly Met Glu Ser Lys Gln Val Asp Tyr Val Pro Leu Arg Ser

1970 1975 1980

Ala Thr Cys Ile Thr Arg Cys Asn Leu Gly Gly Ala Val Cys Leu Lys

1985 1990 1995 2000

His Ala Glu Glu Tyr Arg Glu Tyr Leu Glu Ser Tyr Asn Thr Ala Thr

2005 2010 2015

Thr Ala Gly Phe Thr Phe Trp Val Tyr Lys Thr Phe Asp Phe Tyr Asn

2020 2025 2030

Leu Trp Asn Thr Phe Thr Arg Leu Gln Ser Leu Glu Asn Val Val Tyr

2035 2040 2045

Asn Leu Val Asn Ala Gly His Phe Asp Gly Arg Ala Gly Glu Leu Pro

2050 2055 2060

Cys Ala Val Ile Gly Glu Lys Val Ile Ala Lys Ile Gln Asn Glu Asp

2065 2070 2075 2080

Val Val Val Phe Lys Asn Asn Thr Pro Phe Pro Thr Asn Val Ala Val

2085 2090 2095

Glu Leu Phe Ala Lys Arg Ser Ile Arg Pro His Pro Glu Leu Lys Leu

2100 2105 2110

Phe Arg Asn Leu Asn Ile Asp Val Cys Trp Ser His Val Leu Trp Asp

2115 2120 2125

Tyr Ala Lys Asp Ser Val Phe Cys Ser Ser Thr Tyr Lys Val Cys Lys

2130 2135 2140

Tyr Thr Asp Leu Gln Cys Ile Glu Ser Leu Asn Val Leu Phe Asp Gly

2145 2150 2155 2160

Arg Asp Asn Gly Ala Leu Glu Ala Phe Lys Lys Cys Arg Asn Gly Val

2165 2170 2175

Tyr Ile Asn Thr Thr Lys Ile Lys Ser Leu Ser Met Ile Lys Gly Pro

2180 2185 2190

Gln Arg Ala Asp Leu Asn Gly Val Val Val Glu Lys Val Gly Asp Ser

2195 2200 2205

Asp Val Glu Phe Trp Phe Ala Val Arg Lys Asp Gly Asp Asp Val Ile

2210 2215 2220

Phe Ser Arg Thr Gly Ser Leu Glu Pro Ser His Tyr Arg Ser Pro Gln

2225 2230 2235 2240

Gly Asn Pro Gly Gly Asn Arg Val Gly Asp Leu Ser Gly Asn Glu Ala

2245 2250 2255

Leu Ala Arg Gly Thr Ile Phe Thr Gln Ser Arg Leu Leu Ser Ser Phe

2260 2265 2270

Thr Pro Arg Ser Glu Met Glu Lys Asp Phe Met Asp Leu Asp Asp Asp

2275 2280 2285

Val Phe Ile Ala Lys Tyr Ser Leu Gln Asp Tyr Ala Phe Glu His Val

2290 2295 2300

Val Tyr Gly Ser Phe Asn Gln Lys Ile Ile Gly Gly Leu His Leu Leu

2305 2310 2315 2320

Ile Gly Leu Ala Arg Arg Gln Gln Lys Ser Asn Leu Val Ile Gln Glu

2325 2330 2335

Phe Val Thr Tyr Asp Ser Ser Ile His Ser Tyr Phe Ile Thr Asp Glu

2340 2345 2350

Asn Ser Gly Ser Ser Lys Ser Val Cys Thr Val Ile Asp Leu Leu Leu

2355 2360 2365

Asp Asp Phe Val Asp Ile Val Lys Ser Leu Asn Leu Lys Cys Val Ser

2370 2375 2380

Lys Val Val Asn Val Asn Val Asp Phe Lys Asp Phe Gln Phe Met Leu

2385 2390 2395 2400

Trp Cys Asn Glu Glu Lys Val Met Thr Phe Tyr Pro Arg Leu Gln Ala

2405 2410 2415

Ala Ala Asp Trp Lys Pro Gly Tyr Val Met Pro Val Leu Tyr Lys Tyr

2420 2425 2430

Leu Glu Ser Pro Leu Glu Arg Val Asn Leu Trp Asn Tyr Gly Lys Pro

2435 2440 2445

Ile Thr Leu Pro Thr Gly Cys Met Met Asn Val Ala Lys Tyr Thr Gln

2450 2455 2460

Leu Cys Gln Tyr Leu Ser Thr Thr Thr Leu Ala Val Pro Ala Asn Met

2465 2470 2475 2480

Arg Val Leu His Leu Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly

2485 2490 2495

Ser Ala Val Leu Arg Gln Trp Leu Pro Ala Gly Ser Ile Leu Val Asp

2500 2505 2510

Asn Asp Val Asn Pro Phe Val Ser Asp Ser Val Ala Ser Tyr Tyr Gly

2515 2520 2525

Asn Cys Ile Thr Leu Pro Phe Asp Cys Gln Trp Asp Leu Ile Ile Ser

2530 2535 2540

Asp Met Tyr Asp Pro Leu Thr Lys Asn Ile Gly Glu Tyr Asn Val Ser

2545 2550 2555 2560

Lys Asp Gly Phe Phe Thr Tyr Leu Cys His Leu Ile Arg Asp Lys Leu

2565 2570 2575

Ala Leu Gly Gly Ser Val Ala Ile Lys Ile Thr Glu Phe Ser Trp Asn

2580 2585 2590

Ala Glu Leu Tyr Ser Leu Met Gly Lys Phe Ala Phe Trp Thr Ile Phe

2595 2600 2605

Cys Thr Asn Val Asn Ala Ser Ser Ser Glu Gly Phe Leu Ile Gly Ile

2610 2615 2620

Asn Trp Leu Asn Lys Thr Arg Thr Glu Ile Asp Gly Lys Thr Met His

2625 2630 2635 2640

Ala Asn Tyr Leu Phe Trp Arg Asn Ser Thr Met Trp Asn Gly Gly Ala

2645 2650 2655

Tyr Ser Leu Phe Asp Met Ser Lys Phe Pro Leu Lys Ala Ala Gly Thr

2660 2665 2670

Ala Val Val Ser Leu Lys Pro Asp Gln Ile Asn Asp Leu Val Leu Ser

2675 2680 2685

Leu Ile Glu Lys Gly Lys Leu Leu Val Arg Asp Thr Arg Lys Glu Val

2690 2695 2700

Phe Val Gly Asp Ser Leu Val Asn Val Lys

2705 2710

<210> 33

<211> 29844

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX191_Δ_N_RNA

<400> 33

gtataagagt gattggcgtc cgtacgtacc ctctcaactc taaaactctt gtagtttaaa 60

tctaatctaa actttataaa cggcacttcc tgcgtgtcca tgcccgcggg cctggtcttg 120

tcatagtgct gacatttgta gttccttgac tttcgttctc tgccagtgac gtgtccattc 180

ggcgccagca gcccacccat aggttgcata atggcaaaga tgggcaaata cggcctgggc 240

ttcaaatggg ccccagaatt tccatggatg cttccgaacg catcggagaa gttgggtaac 300

cctgagaggt cagaggagga tgggttttgc ccctctgctg cgcaagaacc gaaagttaaa 360

ggaaaaactt tggttaatca cgtgagggtg aattgtagcc ggcttccagc tttggaatgc 420

tgtgttcagt ctgccataat ccgtgatatt tttgtagatg aggatcccca gaaggtggag 480

gcctcaacta tgatggcatt gcagttcggt agtgccgtct tggttaagcc atccaagcgc 540

ttgtctattc aggcatggac taatttgggt gtgcttccca aaacagctgc catggggttg 600

ttcaagcgcg tctgcctgtg taacaccagg gagtgctctt gtgacgccca cgtggccttt 660

caccttttta cggtccaacc cgatggtgta tgcctgggta atggccgttt tataggctgg 720

ttcgttccag tcacagccat accggagtat gcgaagcagt ggttgcaacc ctggtccatc 780

cttcttcgta agggtggtaa caaagggtct gtgacatccg gccacttccg ccgcgctgtt 840

accatgcctg tgtatgactt taatgtagag gatgcttgtg aggaggttca tcttaacccg 900

aagggtaagt actcctgcaa ggcgtatgcc ctgctgaagg gctatcgcgg tgttaagccc 960

atcctgtttg tggaccagta tggttgcgac tatactggat gtctcgccaa gggtcttgag 1020

gactatggcg atctcacctt gagtgagatg aaggagttgt tccctgtgtg gcgtgactcc 1080

ttggatagtg aagtccttgt ggcttggcac gttgatcgag atcctcgggc tgctatgcgt 1140

ctgcagactc ttgctactgt acgttgcatt gattatgtgg gccaaccgac cgaggatgtg 1200

gtggatggag atgtggtagt gcgtgagcct gctcatcttc tcgcagccaa tgccattgtt 1260

aaaagactcc cccgtttggt ggagactatg ctgtatacgg attcgtccgt tacagaattc 1320

tgttataaaa ccaagctgtg tgaatgcggt tttatcacgc agtttggcta tgtggattgt 1380

tgtggtgaca cctgtgattt tcgtgggtgg gttgccggca atatgatgga tggctttcca 1440

tgtccagggt gtaccaaaaa ttatatgccc tgggaattgg aggcccagtc atcaggtgtt 1500

ataccagaag gaggtgttct attcactcag agcactgata cagtgaatcg tgagtccttt 1560

aagctctacg gtcatgctgt tgtgcctttt ggttctgctg tgtattggag cccttgccca 1620

ggtatgtggc ttccagtaat ttggtcgtcg gttaagtcat actctggttt gacttataca 1680

ggagtagttg gttgtaaggc aattgttcaa gagacagacg ctatatgtcg ttctctgtat 1740

atggattatg tccagcacaa gtgtggcaat ctcgagcaga gagctatcct tggattggac 1800

gatgtctatc atagacagtt gcttgtgaat aggggtgact atagtctcct ccttgagaat 1860

gtggatttgt ttgttaagcg gcgcgctgaa tttgcttgca aattcgccac ctgtggagat 1920

ggtcttgtac ccctcctact agatggttta gtgccccgca gttattattt gattaagagt 1980

ggtcaagctt tcacctctat gatggttaat tttagccatg aggtgactga catgtgtatg 2040

gacatggctt tattgttcat gcatgatgtt aaagtggcca ctaagtatgt taagaaggtt 2100

actggcaaac tggccgtgcg ctttaaagcg ttgggtgtag ccgttgtcag aaaaattact 2160

gaatggtttg atttagccgt ggacattgct gctagtgccg ctggatggct ttgctaccag 2220

ctggtaaatg gcttatttgc agtggccaat ggtgttataa cctttgtaca ggaggtgcct 2280

gagcttgtca agaattttgt tgacaagttc aaggcatttt tcaaggtttt gatcgactct 2340

atgtcggttt ctatcttgtc tggacttact gttgtcaaga ctgcctcaaa tagggtgtgt 2400

cttgctggca gtaaggttta tgaagttgtg cagaaatctt tgtctgcata tgttatgcct 2460

gtgggttgca gcgaagccac ttgtttggtg ggtgagattg aacctgcagt ttttgaagat 2520

gatgttgttg atgtggttaa agccccatta acatatcaag gctgttgtaa gccacccact 2580

tctttcgaga agatttgtat tgtggataaa ttgtatatgg ccaagtgtgg tgatcaattt 2640

taccctgtgg ttgttgataa cgacactgtt ggcgtgttag atcagtgctg gaggtttccc 2700

tgtgcgggca agaaagtcga gtttaacgac aagcccaaag tcaggaagat accctccacc 2760

cgtaagatta agatcacctt cgcactggat gcgacctttg atagtgttct ttcgaaggcg 2820

tgttcagagt ttgaagttga taaagatgtt acattggatg agctgcttga tgttgtgctt 2880

gacgcagttg agagtacgct cagcccttgt aaggagcatg atgtgatagg cacaaaagtt 2940

tgtgctttac ttgataggtt ggcaggagat tatgtctatc tttttgatga gggaggcgat 3000

gaagtgatcg ccccgaggat gtattgttcc ttttctgctc ctgatgacga ggactgcgtt 3060

gcagcggatg ttgtagatgc agatgaaaac caagatgatg atgccgagga ctcagcagtc 3120

cttgtcgctg atacccaaga agaggacggc gttgccaagg ggcaggttga ggcggattcg 3180

gaaatttgcg ttgcgcatac tggtagtcaa gaagaattgg ctgagcctga tgctgtcgga 3240

tctcaaactc ccatcgcctc tgctgaggaa accgaagtcg gagaggcaag cgacagggaa 3300

gggattgctg aggcgaaggc aactgtgtgt gctgatgctg tagatgcctg ccccgatcaa 3360

gtggaggcat ttgaaattga aaaggtcgag gactctatct tggatgagct tcaaactgaa 3420

cttaatgcgc cagcggacaa gacctatgag gatgtcttgg cattcgatgc cgtatgctca 3480

gaggcgttgt ctgcattcta tgctgtgccg agtgatgaga cgcactttaa agtgtgtgga 3540

ttctattcgc ctgctataga gcgcactaat tgttggctgc gttctacttt gatagtaatg 3600

cagagtctac ctttggaatt taaagacttg gagatgcaaa agctctggtt gtcttacaag 3660

gccggctatg accaatgctt tgtggacaaa ctagttaaga gcgtgcccaa gtctattatc 3720

cttccacaag gtggttatgt ggcagatttt gcctatttct ttctaagcca gtgtagcttt 3780

aaagcttatg ctaactggcg ttgtttagag tgtgacatgg agttaaagct tcaaggcttg 3840

gacgccatgt ttttctatgg ggacgttgtg tctcatatgt gcaagtgtgg taatagcatg 3900

accttgttgt ctgcagatat accctacact ttgcattttg gagtgcgaga tgataagttt 3960

tgcgcttttt acacgccaag aaaggtcttt agggctgctt gtgcggtaga tgttaatgat 4020

tgtcactcta tggctgtagt agagggcaag caaattgatg gtaaagtggt taccaaattt 4080

attggtgaca aatttgattt tatggtgggt tacgggatga catttagtat gtctcctttt 4140

gaactcgccc agttatatgg ttcatgtata acaccaaatg tttgttttgt taaaggagat 4200

gttataaagg ttgttcgctt agttaatgct gaagtcattg ttaaccctgc taatgggcgt 4260

atggctcatg gtgccggcgt cgccggcgcc atagctgaaa aggcgggcag tgcttttatt 4320

aaagaaacct ccgatatggt gaaggctcag ggcgtttgcc aggttggtga atgctatgaa 4380

tctgccggtg gtaagttatg taaaaaggtg cttaacattg tagggccaga tgcgcgaggg 4440

catggcaagc aatgctattc acttttagag cgtgcttatc agcatattaa taagtgtgac 4500

aatgttgtca ctactttaat ttcggctggt atatttagtg tgcctactga tgtctcccta 4560

acttacttac ttggtgtagt gacaaagaat gtcattcttg tcagtaacaa ccaggatgat 4620

tttgatgtga tagagaagtg tcaggtgacc tccgttgctg gtaccaaagc gctatcactt 4680

caattggcca aaaatttgtg ccgtgatgta aagtttgtga cgaatgcatg tagttcgctt 4740

tttagtgaat cttgctttgt ctcaagctat gatgtgttgc aggaagttga agcgctgcga 4800

catgatatac aattggatga tgatgctcgt gtctttgtgc aggctaatat ggactgtctg 4860

cccacagact ggcgtctcgt taacaaattt gatagtgttg atggtgttag aaccattaag 4920

tattttgaat gcccgggcgg gatttttgta tccagccagg gcaaaaagtt tggttatgtt 4980

cagaatggtt catttaagga ggcgagtgtt agccaaataa gggctttact cgctaataag 5040

gttgatgtct tgtgtactgt tgatggtgtt aacttccgct cctgctgcgt agcagagggt 5100

gaagtttttg gcaagacatt aggttcagtc ttttgtgatg gcataaatgt caccaaagtt 5160

aggtgtagtg ccatttacaa gggtaaggtt ttctttcagt acagtgattt gtccgaggca 5220

gatcttgtgg ctgttaaaga tgcctttggt tttgatgaac cacaactgct gaagtactac 5280

actatgcttg gcatgtgtaa gtggccagta gttgtttgtg gcaattattt tgctttcaag 5340

cagtcaaata ataattgcta catcaacgtg gcatgtttaa tgctgcaaca cttgagttta 5400

aagtttccta agtggcaatg gcaagaggct tggaacgagt tccgctctgg taaaccacta 5460

aggtttgtgt ccttggtatt agcaaagggc agctttaaat ttaatgaacc ttctgattct 5520

atcgatttta tgcgtgtggt gctacgtgaa gcagatttga gtggtgccac gtgcaatttg 5580

gaatttgttt gtaaatgtgg tgtgaagcaa gagcagcgca aaggtgttga cgctgttatg 5640

cattttggta cgttggataa aggtgatctt gtcaggggtt ataatatcgc atgtacgtgc 5700

ggtagtaaac ttgtgcattg cacccaattt aacgtaccat ttttaatttg ctccaacaca 5760

ccagagggta ggaaactgcc cgacgatgtt gttgcagcta atatttttac tggtggtagt 5820

gtgggccatt acacgcatgt gaaatgtaaa cccaagtacc agctttatga tgcttgtaat 5880

gttaataagg tttcggaggc taagggtaat tttaccgatt gcctctacct taaaaattta 5940

aagcaaacct tctcgtctgt gctgacgact ttttatttag atgacgtaaa gtgtgtggag 6000

tataagccag atttatcgca gtattactgt gagtctggta aatattatac aaaacccatt 6060

attaaggccc aatttagaac atttgagaag gttgatggtg tctataccaa ctttaaattg 6120

gtgggacata gtattgctga aaaactcaat gctaagctgg gatttgattg taattctccc 6180

tttgtggagt ataaaattac agagtggcca acagctactg gagatgtggt gttggctagt 6240

gatgatttgt atgtaagtcg gtacttaagc gggtgcatta cttttggtaa accggttgtc 6300

tggcttggcc atgaggaagc atcgctgaaa tctctcacat attttaatag acctagtgtc 6360

gtttgtgaaa ataaatttaa cgtgttgccc gttgatgtca gtgaacccac ggacaagggg 6420

cctgtgcctg ctgcagtcct tgttaccggc gtccctggag ctgatgcgtc agctggtgcc 6480

ggtattgcca aggagcaaaa agcctgtgct tctgctagtg tggaggatca ggttgttacg 6540

gaggttcgtc aagagccatc tgtttcagct gctgatgtca aagaggttaa attgaatggt 6600

gttaaaaagc ctgttaaggt ggaaggtagt gtggttgtta atgatcccac tagcgaaacc 6660

aaagttgtta aaagtttgtc tattgttgat gtctatgata tgttcctgac agggtgtaag 6720

tatgtggttt ggactgctaa tgagttgtct cgactagtaa attcaccgac tgttagggag 6780

tatgtgaagt ggggtatggg aaagattgta acacccgcta agttgttgtt gttaagagat 6840

gagaagcaag agttcgtagc gccaaaagta gtcaaggcga aagctattgc ctgctattgt 6900

gctgtgaagt ggtttctcct ctattgtttt agttggataa agtttaatac tgacaataag 6960

gttatataca ccacagaagt agcttcaaag cttactttca agttgtgctg tttggccttt 7020

aagaatgcct tacagacgtt taattggagc gttgtgtcta ggggcttttt cctagttgca 7080

acggtctttt tactctggtt taactttttg tatgctaatg ttattttgag tgacttctat 7140

ttgcctaata ttgggcctct ccctacgttt gtgggacaga tagttgcgtg gtttaagact 7200

acatttggtg tgtcaaccat ctgtgatttc taccaggtga cggatttggg ctatagaagt 7260

tcgttttgta atggaagtat ggtatgtgaa ctatgcttct caggttttga tatgctggac 7320

aactatgatg ctataaatgt tgttcaacac gttgtagata ggcgtttgtc ctttgactat 7380

attagcctat ttaaactggt agttgagctt gtaatcggct actctcttta tactgtgtgc 7440

ttctacccac tgtttgtcct tattggaatg cagttattga ccacatggtt gcctgaattc 7500

tttatgctgg agactatgca ttggagtgct cgtttgtttg tgtttgttgc caatatgctt 7560

ccagctttta cgttactgcg attttacatc gtggtgacag ctatgtataa ggtctattgt 7620

ctttgtagac atgttatgta tggatgtagt aagcctggtt gcttgttttg ttataagaga 7680

aaccgtagtg tccgtgttaa gtgtagcacc gttgttggtg gttcactacg ctattacgat 7740

gtaatggcta acggcggcac aggtttctgt acaaagcacc agtggaactg tcttaattgc 7800

aattcctgga aaccaggcaa tacattcata actcatgaag cagcggcgga cctctctaag 7860

gagttgaaac gccctgtgaa tccaacagat tctgcttatt actcggtcac agaggttaag 7920

caggttggtt gttccatgcg tttgttctac gagagagatg gacagcgtgt ttatgatgat 7980

gttaatgcta gtttgtttgt ggacatgaat ggtctgctgc attctaaagt taaaggtgtg 8040

cctgaaacgc atgttgtggt tgttgagaat gaagctgata aagctggttt tctcggcgcc 8100

gcagtgtttt atgcacaatc gctctacaga cctatgttga tggtggaaaa gaaattaata 8160

actaccgcca acactggttt gtctgttagt cgaactatgt ttgaccttta tgtagattca 8220

ttgctgaacg tcctcgacgt ggatcgcaag agtctaacaa gttttgtaaa tgctgcgcac 8280

aactctctaa aggagggtgt tcagcttgaa caagttatgg atacctttat tggctgtgcc 8340

cgacgtaagt gtgctataga ttctgatgtt gaaaccaagt ctattaccaa gtccgtcatg 8400

tcggcagtaa atgctggcgt tgattttacg gatgagagtt gtaataactt ggtgcctacc 8460

tatgttaaaa gtgacactat cgttgcagcc gatttgggtg ttcttattca gaataatgct 8520

aagcatgtac aggctaatgt tgctaaagcc gctaatgtgg cttgcatttg gtctgtggat 8580

gcttttaacc agctatctgc tgacttacag cataggctgc gaaaagcatg ttcaaaaact 8640

ggcttgaaga ttaagcttac ttataataag caggaggcaa atgttcctat tttaactaca 8700

ccgttctctc ttaaaggggg cgctgttttt agtagaatgt tacaatggtt gtttgttgct 8760

aatttgattt gtttcattgt gttgtgggcc cttatgccaa catatgcagt gcacaaatcg 8820

gatatgcagt tgcctttata tgccagtttt aaagttatag ataacggtgt gctaagggat 8880

gtgtctgtta ctgacgcatg cttcgcaaac aaatttaatc aattcgacca atggtatgag 8940

tctacttttg gtcttgctta ttaccgcaac tctaaggctt gtcctgttgt ggttgctgta 9000

atagatcaag acattggcca taccttattt aatgttccta ccacagtttt aagatatgga 9060

tttcatgtgt tgcattttat aacccatgca tttgctactg atagcgtgca gtgttacacg 9120

ccacatatgc aaatccccta tgataatttc tatgctagtg gttgcgtgtt gtcatccctc 9180

tgtactatgc ttgcgcatgc agatggaacc ccgcatcctt attgttatac agggggtgtt 9240

atgcataatg cctctctgta tagttctttg gctcctcatg tccgttataa cctggctagt 9300

tcaaatggtt atatacgttt tcccgaagtg gttagtgaag gcattgtgcg tgttgtgcgc 9360

actcgctcta tgacctactg cagggttggt ttatgtgagg aggccgagga gggtatctgc 9420

tttaatttta atcgttcatg ggtattgaac aacccgtatt atagggccat gcctggaact 9480

ttttgtggta ggaatgcttt tgatttaata catcaagttt taggaggatt agtgcggcct 9540

attgatttct ttgccttaac ggcgagttca gtggctggtg ctatccttgc aattattgtc 9600

gttttggctt tctattattt aatcaagctt aagcgtgcct ttggtgacta cactagtgtt 9660

gtggttatca atgtaattgt gtggtgtata aattttctga tgctttttgt gtttcaggtt 9720

tatcccacat tgtcttgttt atatgcttgt ttctacttct acaccacgct ttatttccct 9780

tcggagataa gtgttgttat gcatttgcaa tggcttgtca tgtatggtgc tattatgccc 9840

ttgtggtttt gcattattta cgtggcagtc gttgtttcaa accatgcatt gtggttgttc 9900

tcttactgcc gcaaaattgg taccgaggtt cgtagtgacg gcacatttga ggaaatggcc 9960

cttactacct ttatgattac taaagaatct tattgtaagt tgaaaaactc tgtttctgat 10020

gttgctttta acaggtactt gagtctttac aacaagtacc gttacttcag tggcaaaatg 10080

gatactgccg cttatagaga ggctgcctgt tcacaactgg caaaggcaat ggaaacattt 10140

aaccataata atggtaatga tgttctctat cagcctccaa ccgcctctgt tactacatca 10200

tttttacagt ctggtatagt gaagatggtg tcgcccacct ctaaagtgga gccttgtatt 10260

gttagtgtta cttatggtaa catgacactt aatgggttgt ggttggatga taaagtttat 10320

tgcccaagac atgttatctg ttcttcagct gacatgacag accctgatta tcctaatttg 10380

ctttgtagag tgacatcaag tgatttttgt gttatgtctg gtcgtatgag ccttactgta 10440

atgtcttatc aaatgcaggg ctgccaactt gttttgactg ttacactgca aaatcctaac 10500

acgcctaagt attccttcgg tgttgttaag cctggtgaga catttactgt actggctgca 10560

tacaatggca gacctcaagg agccttccat gttacgcttc gtagtagcca taccataaag 10620

ggctcctttc tatgtggatc ctgcggttct gtaggatatg ttttaactgg cgatagtgta 10680

cgatttgttt atatgcatca gctagagttg agtactggtt gtcataccgg tactgacttt 10740

agtgggaact tttatggtcc ctatagagat gcgcaagttg tacaattgcc tgttcaggat 10800

tatacgcaga ctgttaatgt tgtagcttgg ctttatgctg ctatttttaa cagatgcaac 10860

tggtttgtgc aaagtgatag ttgttccctg gaggagttta atgtttgggc tatgaccaat 10920

ggttttagct caatcaaagc cgatcttgtc ttggatgcgc ttgcttctat gacaggcgtt 10980

acagttgaac aggtgttggc cgctattaag aggctgcatt ctggattcca gggcaaacaa 11040

attttaggta gttgtgtgct tgaagatgag ctgacaccaa gtgatgttta tcaacaacta 11100

gctggtgtca agctacagtc aaagcgcaca agagttataa aaggtacatg ttgctggata 11160

ttggcttcaa cgtttttgtt ctgtagcatt atctcagcat ttgtaaaatg gactatgttt 11220

atgtatgtta ctacccatat gttgggagtg acattgtgtg cactttgttt tgtaagcttt 11280

gctatgttgt tgatcaagca taagcatttg tatttaacta tgtacatcat gcctgtgtta 11340

tgcacactgt tttacaccaa ctatttggtt gtgtacaaac agagttttag aggtctagct 11400

tatgcttggc tttcacactt tgtccctgct gtagattata catatatgga tgaagtttta 11460

tatggtgttg tgttgctagt agctatggtg tttgttacca tgcgtagcat aaaccacgac 11520

gtcttttcta ttatgttctt ggttggtaga cttgtcagcc tggtatccat gtggtatttt 11580

ggagccaatt tagaggaaga ggtactattg ttcctcacat ccctatttgg cacgtacaca 11640

tggactacta tgttgtcatt ggctaccgct aaggttattg ctaaatggtt ggctgtgaat 11700

gtcttgtact tcacagacgt accgcaaatt aaattagttc tgttgagcta cttgtgtatt 11760

ggttatgtgt gttgttgtta ttggggaatc ttgtcactcc ttaatagcat ttttaggatg 11820

ccattgggcg tctacaatta taaaatctcc gttcaggagt tacgttatat gaatgctaat 11880

ggcttgcgcc cacctagaaa tagttttgag gccctgatgc ttaattttaa gctgttggga 11940

attggtggtg tgccagtcat tgaagtatct caaattcaat caagattgac ggatgttaaa 12000

tgtgctaatg ttgtgttgct taattgcctc cagcacttgc atattgcatc taattctaag 12060

ttgtggcagt attgtagtac tttgcacaat gaaatactgg ctacatctga tttgagcgtg 12120

gccttcgata agttggctca actcttagtt gttttatttg ctaatccagc agcagtggat 12180

agcaagtgcc ttgcaagtat tgaagaagtg agcgatgatt acgttcgcga caatactgtc 12240

ttgcaagcct tacagagtga atttgttaat atggctagct tcgttgagta tgaacttgct 12300

aagaagaatc tagatgaggc taaggctagc ggctctgcca atcaacagca gattaagcag 12360

ctagagaagg cgtgtaatat tgctaagtca gcatatgagc gcgacagagc tgttgctcgt 12420

aagctggaac gtatggctga tttagctctt acaaacatgt ataaagaagc tagaattaat 12480

gataagaaga gtaaggtagt gtctgcattg caaaccatgc tctttagtat ggtgcgtaag 12540

ctagataacc aagctcttaa ttctatttta gacaacgcag ttaagggttg tgtacctttg 12600

aatgcaatac catcattgac ttcgaacact ctgactataa tagtgccaga taagcaggtt 12660

tttgatcagg ttgtggataa tgtgtatgtc acctatgctg ggaatgtatg gcatatacag 12720

tttattcaag atgctgatgg tgctgttaaa caattgaatg agatagatgt taattcaacc 12780

tggcctctag tcattgctgc aaataggcat aatgaagtgt ctactgttgt tttgcagaac 12840

aatgagttga tgcctcagaa gttgagaact caggttgtca atagtggctc agatatgaat 12900

tgtaatactc ctacccagtg ttactataat actactggca cgggtaagat tgtgtatgct 12960

atacttagtg actgtgacgg cctgaagtac actaagatag taaaagaaga tggaaattgt 13020

gttgttttgg aattggatcc tccctgtaag ttttctgttc aggatgtgaa gggccttaaa 13080

attaagtacc tttactttgt gaaggggtgt aatacactgg ctagaggctg ggttgtaggc 13140

accttatcct cgacagtgag attgcaggcg ggtacggcaa ctgagtatgc ctccaactct 13200

gcaatactgt cgctgtgtgc gttttctgta gatcctaaga aaacgtactt ggattatata 13260

aaacagggtg gagttcccgt tactaattgt gttaagatgt tatgtgacca tgctggcact 13320

ggtatggcca ttactattaa gccggaggca accactaatc aggattctta tggtggtgct 13380

tccgtttgta tatattgccg ctcgcgtgtt gaacatccag atgttgatgg attgtgcaaa 13440

ttacgcggca agtttgtcca agtgccctta ggcataaaag atcctgtgtc atatgtgttg 13500

acgcatgatg tttgtcaggt ttgtggcttt tggcgagatg gtagctgttc ctgtgtaggc 13560

acaggctccc agtttcagtc aaaagacacg aactttttaa acggattcgg ggtacaagtg 13620

taaatgcccg tcttgtaccc tgtgccagtg gcttggacac tgatgttcaa ttaagggcat 13680

ttgacatttg taatgctaat cgagctggca ttggtttgta ttataaagtg aattgctgcc 13740

gcttccagcg tgtagatgag gacggcaaca agttggataa gttctttgtt gttaaaagaa 13800

ctaatttaga agtgtataac aaggagaaag aatgctatga gttgacaaaa gaatgcggtg 13860

ttgtggctga acacgagttc ttcacatttg atgtggaggg aagtcgggta ccacacatag 13920

tccgtaaaga tctttcaaag tttactatgt tagatctttg ctatgcattg cgtcattttg 13980

accgcaatga ttgttcaact cttaaggaaa ttctccttac atatgctgag tgtgaagagt 14040

cctacttcca aaagaaggac tggtatgatt ttgttgagaa tcctgatata attaatgtgt 14100

acaagaagct tggtcctata tttaatagag ccctgcttaa cactgccaag tttgcagacg 14160

cattagtgga ggcaggctta gtaggtgttt taacacttga taatcaagat ttatatggtc 14220

aatggtatga ctttggagat tttgtcaaga cagtacctgg ttgtggtgtt gccgtggcag 14280

actcttatta ttcatatatg atgccaatgc tgactatgtg tcatgcgttg gatagtgagt 14340

tgtttgttaa tggtacttat agggagtttg accttgttca gtatgatttt actgatttca 14400

agctagagct gttcactaag tattttaagc attggagtat gacctaccac ccgaacacct 14460

gtgagtgcga ggatgacagg tgcattattc attgcgccaa ttttaatata cttttcagca 14520

tggtcttacc taagacctgt tttgggcctc ttgttaggca gatatttgtg gatggtgttc 14580

ctttcgttgt gtcgatcggt taccattata aagaattagg tgttgttatg aatatggatg 14640

tggatacaca tcgttatcgc ttgtctctta aggacttgct tttgtatgct gcagaccctg 14700

cccttcatgt ggcgtctgct agtgcactgc ttgatttgcg cacatgttgt tttagcgttg 14760

cagctattac aagtggcgta aaatttcaaa cagttaaacc tggaaatttt aatcaggatt 14820

tctacgagtt tattttgagt aaaggcctgc ttaaagaggg gagctccgtt gatttgaagc 14880

acttcttctt tacgcaggat ggtaatgctg ctattactga ttacaattac tacaagtata 14940

atctacccac catggtggat attaagcagt tgttgtttgt tttagaagtt gttaataagt 15000

acttcgagat ctatgagggt gggtgtatac ccgcaacaca ggtcattgtt aataattatg 15060

acaagagtgc tggctatcca tttaataaat ttggaaaggc caggctctat tatgaggcat 15120

tatcatttga ggagcaggat gaaatttatg cgtataccaa acgcaatgtc ctgccgaccc 15180

taactcaaat gaatcttaaa tatgctatta gtgctaagaa tagggcccgc accgttgctg 15240

gtgtctctat tctcagtact atgactggca gaatgtttca tcaaaagtgt ctaaagagta 15300

tagcagctac tcgcggtgtt cctgtagtta taggcaccac gaagttctat ggcggttggg 15360

atgatatgtt acgccgcctt attaaagatg ttgatagtcc tgtactcatg ggttgggact 15420

atcctaaatg tgatcgtgct atgccaaaca tactgcgtat tgttagtagt ttggtgctag 15480

cccgtaaaca tgattcgtgc tgttcgcata cggatagatt ctatcgtctt gcgaacgagt 15540

gcgcccaagt tttgagtgaa attgttatgt gtggtggttg ttattatgtt aaaccaggtg 15600

gcactagtag tggggatgca accactgctt ttgctaattc tgtgtttaac atttgtcaag 15660

ctgtttccgc caatgtatgc tcgcttatgg catgcaatgg acacaaaatt gaagatttga 15720

gtatacgcga gttacaaaag cgcctatact ctaatgtcta tcgtgcggac catgttgacc 15780

ccgcatttgt tagtgagtat tatgagtttt taaacaagca ttttagtatg atgattttga 15840

gtgatgatgg tgttgtgtgt tataattcag agtttgcgtc caagggttat attgctaata 15900

taagtgcctt tcaacaggta ttatattatc aaaacaacgt gtttatgtct gaggccaaat 15960

gttgggtaga aacagacatc gaaaagggac cgcatgaatt ttgttctcaa catacaatgc 16020

tagtcaagat ggatggtgat gaagtctacc ttccataccc tgatccttcg agaatcttag 16080

gagcaggctg ttttgttgat gatttactca agactgatag cgttctcttg atagagcgtt 16140

tcgtaagtct tgcaattgat gcttatcctt tagtatacca tgagaaccca gagtatcaaa 16200

atgtgttccg ggtatattta gaatacatca agaagctgta caatgatctc ggtaatcaga 16260

tcctggacag ctacagtgtt attttaagta cttgtgatgg tcaaaagttt actgacgaga 16320

cgttttacaa gaacatgtat ttaagaagtg cagtgctgca aagcgttggt gcctgcgttg 16380

tctgtagttc tcaaacatca ttacgttgtg gcagttgcat acgcaagcct ttgctgtgtt 16440

gcaaatgcgc ctatgatcat gttatgtcca ctgatcataa atatgtcctg agtgtgtcac 16500

catatgtgtg taattcaccg ggatgtgatg taaatgatgt taccaaattg tatttaggtg 16560

gtatgtcata ttattgtgag gaccataaac cacagtattc attcaaattg gtgatgaatg 16620

gtatggtttt tggtttatat aagcagtctt gtactggttc gccctacata gaggatttta 16680

ataaaatcgc tagttgcaaa tggacagaag tcgatgatta tgtgctagct aatgaatgca 16740

ccgaacgcct taaattgttt gccgcagaaa cgcagaaggc cacagaagag gcctttaagc 16800

aatgttatgc gtcagcaacg atccgtgaga tcgtgagcga tcgggagtta attttatctt 16860

gggaaattgg taaagtccgc ccgccactta ataaaaatta cgtgttcacc ggctaccatt 16920

ttactaataa tggtaagaca gttttaggtg agtatgtttt tgataagagt gagttgacta 16980

atggtgtgta ttatcgcgcc acaaccactt ataagttatc tgtaggtgat gtgttcattt 17040

taacatcaca cgcagtgtct agtttaagtg ctcctacatt agtaccgcag gagaattata 17100

ctagcattcg ttttgctagt gtttatagtg tgcctgagac gtttcagaat aatgtgccta 17160

attatcagca cattggaatg aagcgctatt gtactgtaca gggaccgcct ggtactggta 17220

agtcccatct agccattggg ctagctgttt attattgtac agcgcgcgtg gtgtataccg 17280

ctgctagcca tgctgcagtt gacgcgctgt gtgaaaaggc acataaattt ctcaacatca 17340

acgactgcac gcgtattgtt cctgcaaagg tgcgtgtaga ttgttatgat aaattcaagg 17400

tcaatgacac cactcgcaag tatgtgttta ctacaataaa tgcattacct gagttggtga 17460

ctgacattat tgtcgttgat gaagttagta tgcttaccaa ctatgagctg tctgttatta 17520

acagtcgtgt tagggctaag cattatgtgt atattggcga cccggcgcag ttacctgcac 17580

cacgtgtgct actgaataag ggaactctag aacctagata ttttaattcc gttaccaagc 17640

taatgtgttg tttgggtcca gatattttct tgggcacctg ttatagatgc cctaaggaga 17700

ttgtggatac ggtgtcagcc ttggtttata ataataagct gaaggctaaa aatgataata 17760

gctccatgtg ctttaaggtt tattataagg gccagactac acatgagagt tctagtgctg 17820

ttaatatgca gcaaatacat ttaatttcca agtttctgaa ggcaaacccc agttggagta 17880

acgccgtatt tattagtcct tataactcgc agaactatgt tgctaagaga gtcttgggat 17940

tacaaaccca gacagtagac tcagcgcagg gttctgaata tgattttgtt atctactcac 18000

agactgcgga aacagcgcat tctgtcaatg taaatagatt caatgttgct attacacgtg 18060

ctaagaaggg tattctctgt gtcatgagta gtatgcaatt atttgagtct cttaatttta 18120

ctacactgac gttggataag attaacaatc cacgattaca gtgtactaca aatttgttta 18180

aggattgtag caggagctat gtaggatatc acccagccca tgcaccatcc tttttggcag 18240

ttgatgacaa atataaggta ggcggtgatt tagccgtttg ccttaatgtt gctgattctg 18300

ctgtcactta ttcgcggctt atatcactca tgggattcaa gcttgacttg acccttgatg 18360

gttattgtaa gctgtttata actagagatg aagctatcaa acgtgttaga gcctgggttg 18420

gcttcgatgc agaaggtgcc catgcgatac gtgatagcat tgggacaaat ttcccattac 18480

aattaggctt ttcgactgga attgattttg ttgtcgaagc cactggaatg tttgctgaga 18540

gagatggtta tgtctttaaa aaggcagccg cacgagctcc tcctggcgaa caatttaaac 18600

accttatccc acttatgtca agagggcaga aatgggatgt ggttcgcatt agaatagtac 18660

aaatgttgtc agaccaccta gtggatttgg cagacagtgt tgtacttgtg acgtgggctg 18720

ccagctttga gctcacatgt ttgcgatatt tcgctaaagt tggaagagaa gttgtgtgta 18780

gtgtctgcac caagcgtgcg acatgtttta attctagaac tggatactat ggatgctggc 18840

gacatagtta ttcctgtgat tacctgtaca acccactaat agttgacatt caacagtggg 18900

gatatacagg atctttaact agcaatcatg atcctatttg cagcgtgcat aagggtgctc 18960

atgttgcatc atctgatgct atcatgaccc ggtgtctagc tgttcatgat tgcttttgta 19020

agtctgttaa ttggaattta gaatacccca ttatttcaaa tgaggtcagt gttaatacct 19080

cctgcaggtt attgcagcgc gtaatgttta gggctgcgat gctatgcaat aggtatgatg 19140

tgtgttatga cattggcaac cctaaaggtc ttgcctgtgt caaaggatat gattttaagt 19200

tctatgacgc ctcccctgtt gttaagtctg ttaaacagtt tgtttacaaa tacgaggcac 19260

ataaagatca atttttagat ggtttgtgta tgttttggaa ctgcaatgtg gataagtatc 19320

cagcgaatgc agttgtgtgt aggtttgaca cgcgtgtgtt gaacaaatta aatctccctg 19380

gctgtaatgg tggcagtttg tatgttaaca aacatgcatt ccacaccagt ccctttaccc 19440

gggctgcctt cgagaatttg aagcctatgc ctttctttta ttattcagat acgccctgtg 19500

tgtatatgga aggcatggaa tctaagcagg tcgattatgt cccattgaga agcgctacat 19560

gcatcacaag atgcaattta ggtggcgctg tttgtttaaa acatgctgag gagtatcgtg 19620

agtaccttga gtcttacaat acggcaacca cagcgggttt tactttttgg gtctataaga 19680

cttttgattt ttacaacctt tggaatactt ttactaggct ccaaagttta gaaaatgtag 19740

tgtataacct ggtcaacgct ggacactttg atggccgggc gggtgaactg ccttgtgctg 19800

ttataggtga gaaagtcatt gccaagattc aaaatgagga tgtcgtggtc tttaaaaata 19860

acacgccatt ccccactaat gtggctgtcg aattatttgc taagcgcagt attcggcccc 19920

accccgagct taagctcttt agaaatttga atattgacgt gtgctggagt cacgtccttt 19980

gggattatgc taaggatagt gtgttttgca gttcgacgta taaggtctgc aaatacacag 20040

atttacagtg cattgaaagc ttgaatgtac tttttgatgg tcgtgataat ggtgctcttg 20100

aagcttttaa gaagtgccgg aatggcgtct acattaacac gacaaaaatt aaaagtctgt 20160

cgatgattaa aggcccacaa cgtgccgatt tgaatggcgt agttgtggag aaagttggag 20220

attctgatgt ggaattttgg tttgctgtgc gtaaagacgg tgacgatgtt atcttcagcc 20280

gtacagggag ccttgaaccg agccattacc ggagcccaca aggtaatccg ggtggtaatc 20340

gcgtgggtga tctcagcggt aatgaagctc tagcgcgtgg cactatcttt actcaaagca 20400

gattattatc ttctttcaca cctcgatcag agatggagaa agattttatg gatttagatg 20460

atgatgtgtt cattgcaaaa tatagtttac aggactacgc gtttgaacac gttgtttatg 20520

gtagttttaa ccagaagatt attggaggtt tgcatttgct tattggctta gcccgtaggc 20580

agcaaaaatc caatctggta attcaagagt tcgtgacata cgactctagc attcattcgt 20640

actttatcac tgacgagaac agtggtagta gtaagagtgt gtgcactgtt attgatttat 20700

tgttagatga ttttgtggac attgtaaagt ccctgaatct aaagtgtgtg agtaaggttg 20760

ttaatgttaa tgtggatttt aaggacttcc agtttatgtt gtggtgcaat gaggagaagg 20820

tcatgacttt ctatcctcgt ttgcaggctg ctgctgactg gaaacctggt tatgttatgc 20880

ctgtcttata taagtatttg gaatcgcctc tggaaagagt aaacctctgg aattatggca 20940

agccgattac tttacctaca ggatgtatga tgaatgttgc taagtatact caattatgtc 21000

aatatttgag cactacaaca ttagcagttc cggctaatat gcgtgtctta caccttggtg 21060

ccggttcgga taagggtgtt gcccctgggt ctgcagttct taggcagtgg ctaccagcgg 21120

gaagtattct tgtagataat gatgtgaatc catttgtgag tgacagtgtc gcctcatatt 21180

atggaaattg tataacctta ccctttgatt gtcagtggga tctgataatt tctgatatgt 21240

acgaccctct tactaagaac attggggagt acaacgtgag taaagatgga ttctttactt 21300

acctctgtca tttaattcgt gacaagttgg ctctgggtgg cagtgttgcc ataaaaataa 21360

cagagttttc ttggaacgct gagttatata gtttaatggg gaagtttgcg ttctggacaa 21420

tcttttgcac caacgtaaac gcctcttcaa gtgaaggatt tttgattggc ataaattggt 21480

tgaataagac ccgtaccgaa attgacggta aaaccatgca tgccaattat ctgttttgga 21540

gaaatagtac aatgtggaat ggaggggctt acagtctctt tgacatgagt aagttccctt 21600

tgaaagcggc tggtacggct gttgttagcc ttaaaccaga ccaaataaat gacttagtcc 21660

tctccttgat tgagaagggc aagttattag tgcgtgatac acgcaaagaa gtttttgttg 21720

gcgatagcct agtaaatgtc aaataaatct atacttgtcg tggctgtgaa aatggccttt 21780

gctgacaagc ctaatcattt cataaacttt cccctggccc aatttagtgg ctttatgggt 21840

aagtatttaa agctacagtc tcaacttgtg gaaatgggtt tagactgtaa attacagaag 21900

gcaccacatg ttagtattac cctgcttgat attaaagcag accaatacaa acaggtggaa 21960

tttgcaatac aagaaataat agatgatctg gcggcatatg agggagatat tgtctttgac 22020

aaccctcaca tgcttggcag atgccttgtt cttgatgtta gaggatttga agagttgcat 22080

gaagatattg ttgaaattct ccgcagaagg ggttgcacgg cagatcaatc cagacactgg 22140

attccgcact gcactgtggc ccaatttgac gaagaaagag aaacaaaagg aatgcaattc 22200

tatcataaag aacccttcta cctcaagcat aacaacctat taacggatgc tgggcttgag 22260

ctcgtgaaga taggttcttc caaaatagat gggttttatt gtagtgaact gagtgtttgg 22320

tgtggtgaga ggctttgtta taagcctcca acacccaaat tcagtgatat atttggctat 22380

tgctgcatag ataaaatacg tggtgattta gaaataggcg acctgccgca ggatgatgag 22440

gaagcgtggg ccgagctaag ttaccactat caaagaaaca cctacttctt cagacatgtg 22500

cacgataata gcatctattt tcgtaccgtg tgtagaatga agggttgtat gtgttgattt 22560

gtttttacac tattagtgta ataagcttat tattttgttg aaaagggcag gatgtgcata 22620

gctatggctc ctcgcacact gcttttgctg atttgatgtc agctggtgtt tgggttcaat 22680

gaacctctta acatcgtttc acatttaaat gatgactggt ttctatttgg tgacagtcgg 22740

tccgactgta cctatgtaga aaataacggt catcctaaat tagattggct tgacctcgac 22800

ccaaagttgt gtaattcagg aaagatttcc gcaaagagtg gtaactctct ctttaggagt 22860

tttcacttca ctgattttta caattatacg ggtgagggat accaaattgt attttatgaa 22920

ggagttaatt ttagtcccag ccatggcttt aaatgcctgg ctcatggaga taataaaaga 22980

tggatgggca ataaagctcg attttatgcc cgagtgtatg agaagatggc ccaatatagg 23040

agcctatcgt ttgttaatgt gtcttatgcc tatggaggta atgcaaagcc cgcctccatt 23100

tgcaaagaca atactttaac actcaataac cccaccttca tatcgaagga gtctaattat 23160

gttgattact actacgagag tgaggctaat ttcacactag aaggttgtga tgaatttata 23220

gtaccgctct gtggttttaa tggccattcc aagggctcgt cgtcggatgc tgccaataaa 23280

tattatactg actctcagag ttactataat atggatattg gtgtcttata tgggttcaat 23340

tcgaccttgg atgttggcaa cactgctaag gatccgggtc ttgatctcac ttgtaggtat 23400

cttgcattga ctcctggtaa ttataaggct gtgtccttag aatatttgtt aagcttaccc 23460

tcaaaggcta tttgcctcca taagacaaag cgctttatgc ctgtgcaggt agttgactca 23520

aggtggagta gcatccgcca gtcagacaat atgaccgctg cagcctgtca gctgccatat 23580

tgtttctttc gcaacacatc tgcgaattat agtggtggca cacatgatgc gcaccatggt 23640

gattttcatt tcaggcagtt attgtctggt ttgttatata atgtttcctg tattgcccag 23700

cagggtgcat ttctttataa taatgtgtcg tcctcttggc cagcctatgg gtacggtcat 23760

tgtccaacgg cagctaacat tggttatatg gcacctgttt gtatctatga ccctctcccg 23820

gtcatactgc taggtgtgtt attgggtata gctgtgttga ctattgtgtt tctgatgttt 23880

tattttatga cggatagcgg tgttagattg catgaggcat aatctaaaca tgtttgtttt 23940

tcttgtttta ttgccactag tctctagtca gtgtgttaat cttacaacca gaactcaatt 24000

accccctgca tacactaatt ctttcacacg tggtgtttat taccctgaca aagttttcag 24060

atcctcagtt ttacattcaa ctcaggactt gttcttacct ttcttttcca atgttacttg 24120

gttccatgct atacatgtct ctgggaccaa tggtactaag aggtttgata accctgtcct 24180

accatttaat gatggtgttt actttgcttc cactgagaag tctaacataa taagaggctg 24240

gatttttggt actactttag attcgaaaac ccagtcccta cttattgtta ataacgctac 24300

taatgttgtt atcaaagtct gtgaatttca attttgtaac gatccatttt tgggtgttta 24360

ttaccacaaa aacaacaaaa gttggatgga aagtgagttc agagtttatt ctagtgcgaa 24420

taattgcact tttgaatacg tctctcagcc ttttcttatg gaccttgaag gaaaacaggg 24480

taatttcaaa aatcttaggg aatttgtgtt caagaatatt gatggttact tcaagatata 24540

ctctaagcac acgcctatta atttagtgcg tgatctccct cagggttttt cggctttaga 24600

accattggta gatttgccaa taggtattaa catcactagg tttcaaactt tacttgcttt 24660

acatagaagt tatttaactc ctggtgattc ttcttcaggt tggacagctg gtgctgcagc 24720

ttattatgtg ggttatcttc aacctaggac ttttctactg aagtacaatg aaaatggaac 24780

cattacagat gctgtagact gtgcacttga ccctctctca gaaacaaagt gtacgttgaa 24840

atccttcact gtagaaaaag gaatctatca aacttctaac tttagagtcc aaccaacaga 24900

atctattgtt agatttccta acatcacaaa cttgtgccct tttggtgaag tttttaacgc 24960

caccagattt gcatctgttt atgcttggaa caggaagaga atcagcaact gtgttgctga 25020

ttattctgtc ctgtataatt ccgcatcatt ttccactttt aagtgttatg gagtgtctcc 25080

tactaaatta aatgatctct gctttactaa tgtctatgca gattcatttg taattagagg 25140

tgatgaagtc agacaaatcg ctccagggca aactggaaag attgctgatt ataactacaa 25200

attaccagat gattttacag gctgcgttat agcttggaat tctaacaatc ttgattctaa 25260

ggttggtggt aattataatt acctgtacag attgtttagg aagtctaatc tcaaaccttt 25320

tgagagagat atttcaactg aaatctatca ggccggtagc acaccttgta atggtgttga 25380

aggttttaat tgttactttc ctctgcaatc atatggtttc caacccacta atggtgttgg 25440

ttaccaacca tacagagtag tagtactttc ttttgaactt ctacatgcac cagcaactgt 25500

ttgtggacct aaaaagtcta ctaatttggt taagaacaag tgtgtcaatt tcaacttcaa 25560

tggtttaaca ggcacaggtg ttcttactga gtctaacaaa aagtttctgc ctttccaaca 25620

atttggcaga gacattgctg acactactga tgctgttcgt gatccacaaa cacttgagat 25680

tcttgacatt acaccatgtt cttttggtgg tgtcagtgtt ataacaccag gaacaaatac 25740

ttctaaccag gttgctgttc tttatcagga tgttaactgc acagaagtcc ctgttgctat 25800

tcatgcagat caacttactc ctacttggcg tgtttattct acaggttcta atgtttttca 25860

aacacgtgca ggctgtttaa taggggctga acatgtcaac aactcatatg agtgtgacat 25920

acccattggt gcaggtatat gcgctagtta tcagactcag actaattctc ctcggagagc 25980

aagaagtgta gctagtcaat ccatcattgc ctacactatg tcacttggtg cagaaaattc 26040

agttgcttac tctaataact ctattgccat acccacaaat tttactatta gcgttaccac 26100

agaaattcta ccagtgtcta tgaccaagac atcagtagat tgtacaatgt acatttgtgg 26160

tgattcaact gaatgcagca atcttttgtt gcaatatggc agtttttgta cacaattaaa 26220

ccgtgcttta actggaatag ctgttgaaca agacaaaaac acccaagaag tttttgcaca 26280

agtcaaacaa atttacaaga caccaccaat taaagatttt ggcggtttta attttagcca 26340

gatactgcca gatccatcaa aaccaagcaa gaggtcattt attgaagatc tactgttcaa 26400

caaagtgaca cttgcagatg ctggcttcat caaacaatat ggtgattgcc ttggtgatat 26460

tgctgctaga gacctcattt gtgcacaaaa gtttaacggc cttactgttt tgccaccttt 26520

gctcacagat gaaatgattg ctcaatacac ttctgcactg ttagcaggta caatcacttc 26580

tggttggact tttggtgcag gtgctgcatt acaaatacca tttgctatgc aaatggctta 26640

taggtttaat ggtattggag ttacacagaa tgttctctat gagaaccaaa aattgattgc 26700

caaccaattt aatagtgcta ttggcaaaat tcaagactca ctttcttcca cagcaagtgc 26760

acttggaaaa cttcaagatg tggtcaacca aaatgcacaa gctttaaaca cgcttgttaa 26820

acaacttagc tccaattttg gtgcaatttc aagtgtttta aacgacatcc tttcacgtct 26880

tgacaaagtt gaggctgaag tgcaaattga taggttgatc acaggcagac ttcaaagttt 26940

gcagacatat gtgactcaac aattaattag agctgcagaa atcagagctt ctgctaatct 27000

tgctgctact aaaatgtcag agtgtgtact tggacaatca aaaagagttg acttttgcgg 27060

aaagggctat catcttatgt catttcctca gtcagcacct catggtgtcg tctttttgca 27120

tgtgacttat gtccctgcac aagaaaagaa cttcacaact gctcctgcca tttgtcatga 27180

tggaaaagca cactttcctc gtgaaggtgt ctttgtttca aatggcacac actggtttgt 27240

aacacaaagg aatttttatg aaccacaaat cattactaca gacaacacat ttgtgtctgg 27300

taactgtgat gttgtaatag gaattgtcaa caacacagtt tatgatcctt tgcaacctga 27360

attagactca ttcaaggagg agcttgataa atacttcaag aaccatacct caccagatgt 27420

tgatttaggt gacatctctg gcattaatgc ttcagttgta aacattcaga aagaaatcga 27480

ccgcctcaat gaggttgcca agaatttaaa tgaatctctc atcgatctcc aagaacttgg 27540

aaagtatgag cagtatataa aatggccatg gtacatttgg ctaggtttta tagctggctt 27600

gattgccata gtaatggtga caattatgct ttgctgtatg accagttgct gtagttgtct 27660

caagggctgt tgttcttgtg gatcctgctg caaatttgac gaggacgact ctgagccagt 27720

gctcaaagga gtcaaattac attacacata actatcacag cctctcctgg aaagacagaa 27780

aatctaaaca atttatagca ttctcattgc tacctggccc cgtaagaggc agtcatagct 27840

atggccgtgt tggtcctaag gctacattgg ctgctgtctt tattggtcca tttattgtag 27900

catgtatgct aggcattggc ctagtttatt tattgcaatt gcaagttcaa atttttcatg 27960

ttaaggatac catacgtgtg actggcaagc cagccactgt gtcttatact acaagtacac 28020

cagtaacacc gagcgcgacg acgctcgatg gtactacgta tactttaatt agacccacta 28080

gctcttatac aagagtttat cttggtactc caagaggttt tgattatagt acatttgggc 28140

ctaagaccct agattatgtt actaatctaa acctcatctt aattctggtc gtccatatac 28200

ttttaaggca ttgtccaggc atatgaggcc aacagccaca tggatttggc atgtgagtga 28260

tgcatggtta cgccgcacgc gggactttgg tgtcattcgc ctagaagatt tttgttttca 28320

atttaattat agccaacccc gagttggtta ttgtagagtt cctttaaagg cttggtgtag 28380

caaccagggt aaatttgcag cgcagtttac cctaaaaagt tgcgaaaaac caggtcacga 28440

aaaatttatt actagcttca cggcctacgg cagaactgtc caacaggccg ttagcaagtt 28500

agtagaagaa gctgttgatt ttattctttt tagggccacg cagctcgaaa gaaatgttta 28560

atttattcct tacagacaca gtatggtatg tggggcagat tatttttata ttcgcagtgt 28620

gtttgatggt caccataatt gtggttgcct tccttgcgtc tatcaaactt tgtattcaac 28680

tttgcggttt atgtaatact ttggtgctgt ccccttctat ttatttgtat gataggagta 28740

agcagcttta taagtactat aatgaagaaa tgagactgcc cctattagag gtggatgata 28800

tctaatccaa acattatgag tagtactact caggccccag agcccgtcta tcaatggacc 28860

gccgacgagg cagttcaatt ccttaaggaa tggaacttct cgttgggcat tatactactc 28920

tttattacta tcatactaca gttcggttac acgagccgta gcatgtttat ttatgttgtg 28980

aaaatgataa tcttgtggtt aatgtggcca ctgactattg ttttgtgtat tttcaattgc 29040

gtgtatgcgc taaataatgt gtatcttgga ttttctatag tgtttactat agtgtccatt 29100

gtaatctgga tcatgtattt tgtgaacagc ataaggttgt ttatcaggac tggtagctgg 29160

tggagcttca accccgaaac aaacaacctt atgtgtatag atatgaaagg taccgtgtat 29220

gttagaccca ttattgagga ttaccataca ctaacagcca ctattattcg tggccacctc 29280

tacatgcaag gtgttaagct aggcaccggt ttctctttgt ctgacttgcc cgcttatgtt 29340

acagttgcta aggtgtcaca cctttgcact tataagcgcg cattcttaga caaggtagac 29400

ggtgttagcg gttttgctgt ttatgtgaag tccaaggtcg gaaattaccg actgccctca 29460

aacaaaccga gtggcgcgga caccgcattg ttgagaacct aatctaaact ttaaggagag 29520

aatgaatcct atgtcggcgc tcggtggtaa cccctcgcga gaaagtcggg ataggacact 29580

ctctatcaga atggatgtct tgctgtcata acagatagag aaggttgtgg cagaccctgt 29640

atcaattagt tgaaagagat tgcaaaatag agaatgtgtg agagaagtta gcaaggtcct 29700

acgtctaacc ataagaacgg cgataggcgc cccctgggaa cagctcacat cagggtacta 29760

ttcctgcaat gccctagtaa atgaatgaag ttgatcatgg ccaattggaa gaatcacaaa 29820

aaaaaaaaaa aaaacggccg gttt 29844

<210> 34

<211> 27671

<212> DNA

<213> Artificial sequence

<220>

<223> COVAX191_Δ_HEN_RNA

<400> 34

gtataagagt gattggcgtc cgtacgtacc ctctcaactc taaaactctt gtagtttaaa 60

tctaatctaa actttataaa cggcacttcc tgcgtgtcca tgcccgcggg cctggtcttg 120

tcatagtgct gacatttgta gttccttgac tttcgttctc tgccagtgac gtgtccattc 180

ggcgccagca gcccacccat aggttgcata atggcaaaga tgggcaaata cggcctgggc 240

ttcaaatggg ccccagaatt tccatggatg cttccgaacg catcggagaa gttgggtaac 300

cctgagaggt cagaggagga tgggttttgc ccctctgctg cgcaagaacc gaaagttaaa 360

ggaaaaactt tggttaatca cgtgagggtg aattgtagcc ggcttccagc tttggaatgc 420

tgtgttcagt ctgccataat ccgtgatatt tttgtagatg aggatcccca gaaggtggag 480

gcctcaacta tgatggcatt gcagttcggt agtgccgtct tggttaagcc atccaagcgc 540

ttgtctattc aggcatggac taatttgggt gtgcttccca aaacagctgc catggggttg 600

ttcaagcgcg tctgcctgtg taacaccagg gagtgctctt gtgacgccca cgtggccttt 660

caccttttta cggtccaacc cgatggtgta tgcctgggta atggccgttt tataggctgg 720

ttcgttccag tcacagccat accggagtat gcgaagcagt ggttgcaacc ctggtccatc 780

cttcttcgta agggtggtaa caaagggtct gtgacatccg gccacttccg ccgcgctgtt 840

accatgcctg tgtatgactt taatgtagag gatgcttgtg aggaggttca tcttaacccg 900

aagggtaagt actcctgcaa ggcgtatgcc ctgctgaagg gctatcgcgg tgttaagccc 960

atcctgtttg tggaccagta tggttgcgac tatactggat gtctcgccaa gggtcttgag 1020

gactatggcg atctcacctt gagtgagatg aaggagttgt tccctgtgtg gcgtgactcc 1080

ttggatagtg aagtccttgt ggcttggcac gttgatcgag atcctcgggc tgctatgcgt 1140

ctgcagactc ttgctactgt acgttgcatt gattatgtgg gccaaccgac cgaggatgtg 1200

gtggatggag atgtggtagt gcgtgagcct gctcatcttc tcgcagccaa tgccattgtt 1260

aaaagactcc cccgtttggt ggagactatg ctgtatacgg attcgtccgt tacagaattc 1320

tgttataaaa ccaagctgtg tgaatgcggt tttatcacgc agtttggcta tgtggattgt 1380

tgtggtgaca cctgtgattt tcgtgggtgg gttgccggca atatgatgga tggctttcca 1440

tgtccagggt gtaccaaaaa ttatatgccc tgggaattgg aggcccagtc atcaggtgtt 1500

ataccagaag gaggtgttct attcactcag agcactgata cagtgaatcg tgagtccttt 1560

aagctctacg gtcatgctgt tgtgcctttt ggttctgctg tgtattggag cccttgccca 1620

ggtatgtggc ttccagtaat ttggtcgtcg gttaagtcat actctggttt gacttataca 1680

ggagtagttg gttgtaaggc aattgttcaa gagacagacg ctatatgtcg ttctctgtat 1740

atggattatg tccagcacaa gtgtggcaat ctcgagcaga gagctatcct tggattggac 1800

gatgtctatc atagacagtt gcttgtgaat aggggtgact atagtctcct ccttgagaat 1860

gtggatttgt ttgttaagcg gcgcgctgaa tttgcttgca aattcgccac ctgtggagat 1920

ggtcttgtac ccctcctact agatggttta gtgccccgca gttattattt gattaagagt 1980

ggtcaagctt tcacctctat gatggttaat tttagccatg aggtgactga catgtgtatg 2040

gacatggctt tattgttcat gcatgatgtt aaagtggcca ctaagtatgt taagaaggtt 2100

actggcaaac tggccgtgcg ctttaaagcg ttgggtgtag ccgttgtcag aaaaattact 2160

gaatggtttg atttagccgt ggacattgct gctagtgccg ctggatggct ttgctaccag 2220

ctggtaaatg gcttatttgc agtggccaat ggtgttataa cctttgtaca ggaggtgcct 2280

gagcttgtca agaattttgt tgacaagttc aaggcatttt tcaaggtttt gatcgactct 2340

atgtcggttt ctatcttgtc tggacttact gttgtcaaga ctgcctcaaa tagggtgtgt 2400

cttgctggca gtaaggttta tgaagttgtg cagaaatctt tgtctgcata tgttatgcct 2460

gtgggttgca gcgaagccac ttgtttggtg ggtgagattg aacctgcagt ttttgaagat 2520

gatgttgttg atgtggttaa agccccatta acatatcaag gctgttgtaa gccacccact 2580

tctttcgaga agatttgtat tgtggataaa ttgtatatgg ccaagtgtgg tgatcaattt 2640

taccctgtgg ttgttgataa cgacactgtt ggcgtgttag atcagtgctg gaggtttccc 2700

tgtgcgggca agaaagtcga gtttaacgac aagcccaaag tcaggaagat accctccacc 2760

cgtaagatta agatcacctt cgcactggat gcgacctttg atagtgttct ttcgaaggcg 2820

tgttcagagt ttgaagttga taaagatgtt acattggatg agctgcttga tgttgtgctt 2880

gacgcagttg agagtacgct cagcccttgt aaggagcatg atgtgatagg cacaaaagtt 2940

tgtgctttac ttgataggtt ggcaggagat tatgtctatc tttttgatga gggaggcgat 3000

gaagtgatcg ccccgaggat gtattgttcc ttttctgctc ctgatgacga ggactgcgtt 3060

gcagcggatg ttgtagatgc agatgaaaac caagatgatg atgccgagga ctcagcagtc 3120

cttgtcgctg atacccaaga agaggacggc gttgccaagg ggcaggttga ggcggattcg 3180

gaaatttgcg ttgcgcatac tggtagtcaa gaagaattgg ctgagcctga tgctgtcgga 3240

tctcaaactc ccatcgcctc tgctgaggaa accgaagtcg gagaggcaag cgacagggaa 3300

gggattgctg aggcgaaggc aactgtgtgt gctgatgctg tagatgcctg ccccgatcaa 3360

gtggaggcat ttgaaattga aaaggtcgag gactctatct tggatgagct tcaaactgaa 3420

cttaatgcgc cagcggacaa gacctatgag gatgtcttgg cattcgatgc cgtatgctca 3480

gaggcgttgt ctgcattcta tgctgtgccg agtgatgaga cgcactttaa agtgtgtgga 3540

ttctattcgc ctgctataga gcgcactaat tgttggctgc gttctacttt gatagtaatg 3600

cagagtctac ctttggaatt taaagacttg gagatgcaaa agctctggtt gtcttacaag 3660

gccggctatg accaatgctt tgtggacaaa ctagttaaga gcgtgcccaa gtctattatc 3720

cttccacaag gtggttatgt ggcagatttt gcctatttct ttctaagcca gtgtagcttt 3780

aaagcttatg ctaactggcg ttgtttagag tgtgacatgg agttaaagct tcaaggcttg 3840

gacgccatgt ttttctatgg ggacgttgtg tctcatatgt gcaagtgtgg taatagcatg 3900

accttgttgt ctgcagatat accctacact ttgcattttg gagtgcgaga tgataagttt 3960

tgcgcttttt acacgccaag aaaggtcttt agggctgctt gtgcggtaga tgttaatgat 4020

tgtcactcta tggctgtagt agagggcaag caaattgatg gtaaagtggt taccaaattt 4080

attggtgaca aatttgattt tatggtgggt tacgggatga catttagtat gtctcctttt 4140

gaactcgccc agttatatgg ttcatgtata acaccaaatg tttgttttgt taaaggagat 4200

gttataaagg ttgttcgctt agttaatgct gaagtcattg ttaaccctgc taatgggcgt 4260

atggctcatg gtgccggcgt cgccggcgcc atagctgaaa aggcgggcag tgcttttatt 4320

aaagaaacct ccgatatggt gaaggctcag ggcgtttgcc aggttggtga atgctatgaa 4380

tctgccggtg gtaagttatg taaaaaggtg cttaacattg tagggccaga tgcgcgaggg 4440

catggcaagc aatgctattc acttttagag cgtgcttatc agcatattaa taagtgtgac 4500

aatgttgtca ctactttaat ttcggctggt atatttagtg tgcctactga tgtctcccta 4560

acttacttac ttggtgtagt gacaaagaat gtcattcttg tcagtaacaa ccaggatgat 4620

tttgatgtga tagagaagtg tcaggtgacc tccgttgctg gtaccaaagc gctatcactt 4680

caattggcca aaaatttgtg ccgtgatgta aagtttgtga cgaatgcatg tagttcgctt 4740

tttagtgaat cttgctttgt ctcaagctat gatgtgttgc aggaagttga agcgctgcga 4800

catgatatac aattggatga tgatgctcgt gtctttgtgc aggctaatat ggactgtctg 4860

cccacagact ggcgtctcgt taacaaattt gatagtgttg atggtgttag aaccattaag 4920

tattttgaat gcccgggcgg gatttttgta tccagccagg gcaaaaagtt tggttatgtt 4980

cagaatggtt catttaagga ggcgagtgtt agccaaataa gggctttact cgctaataag 5040

gttgatgtct tgtgtactgt tgatggtgtt aacttccgct cctgctgcgt agcagagggt 5100

gaagtttttg gcaagacatt aggttcagtc ttttgtgatg gcataaatgt caccaaagtt 5160

aggtgtagtg ccatttacaa gggtaaggtt ttctttcagt acagtgattt gtccgaggca 5220

gatcttgtgg ctgttaaaga tgcctttggt tttgatgaac cacaactgct gaagtactac 5280

actatgcttg gcatgtgtaa gtggccagta gttgtttgtg gcaattattt tgctttcaag 5340

cagtcaaata ataattgcta catcaacgtg gcatgtttaa tgctgcaaca cttgagttta 5400

aagtttccta agtggcaatg gcaagaggct tggaacgagt tccgctctgg taaaccacta 5460

aggtttgtgt ccttggtatt agcaaagggc agctttaaat ttaatgaacc ttctgattct 5520

atcgatttta tgcgtgtggt gctacgtgaa gcagatttga gtggtgccac gtgcaatttg 5580

gaatttgttt gtaaatgtgg tgtgaagcaa gagcagcgca aaggtgttga cgctgttatg 5640

cattttggta cgttggataa aggtgatctt gtcaggggtt ataatatcgc atgtacgtgc 5700

ggtagtaaac ttgtgcattg cacccaattt aacgtaccat ttttaatttg ctccaacaca 5760

ccagagggta ggaaactgcc cgacgatgtt gttgcagcta atatttttac tggtggtagt 5820

gtgggccatt acacgcatgt gaaatgtaaa cccaagtacc agctttatga tgcttgtaat 5880

gttaataagg tttcggaggc taagggtaat tttaccgatt gcctctacct taaaaattta 5940

aagcaaacct tctcgtctgt gctgacgact ttttatttag atgacgtaaa gtgtgtggag 6000

tataagccag atttatcgca gtattactgt gagtctggta aatattatac aaaacccatt 6060

attaaggccc aatttagaac atttgagaag gttgatggtg tctataccaa ctttaaattg 6120

gtgggacata gtattgctga aaaactcaat gctaagctgg gatttgattg taattctccc 6180

tttgtggagt acaaaattac agagtggcca acagctactg gagatgtggt gttggctagt 6240

gatgatttgt atgtaagtcg gtacttaagc gggtgcatta cttttggtaa accggttgtc 6300

tggcttggcc atgaggaagc atcgctgaaa tctctcacat attttaatag acctagtgtc 6360

gtttgtgaaa ataaatttaa cgtgttgccc gttgatgtca gtgaacccac ggacaagggg 6420

cctgtgcctg ctgcagtcct tgttaccggc gtccctggag ctgatgcgtc agctggtgcc 6480

ggtattgcca aggagcaaaa agcctgtgct tctgctagtg tggaggatca ggttgttacg 6540

gaggttcgtc aagagccatc tgtttcagct gctgatgtca aagaggttaa attgaatggt 6600

gttaaaaagc ctgttaaggt ggaaggtagt gtggttgtta atgatcccac tagcgaaacc 6660

aaagttgtta aaagtttgtc tattgttgat gtctatgata tgttcctgac agggtgtaag 6720

tatgtggttt ggactgctaa tgagttgtct cgactagtaa attcaccgac tgttagggag 6780

tatgtgaagt ggggtatggg aaagattgta acacccgcta agttgttgtt gttaagagat 6840

gagaagcaag agttcgtagc gccaaaagta gtcaaggcga aagctattgc ctgctattgt 6900

gctgtgaagt ggtttctcct ctattgtttt agttggataa agtttaatac tgacaataag 6960

gttatataca ccacagaagt agcttcaaag cttactttca agttgtgctg tttggccttt 7020

aagaatgcct tacagacgtt taattggagc gttgtgtcta ggggcttttt cctagttgca 7080

acggtctttt tactctggtt taactttttg tatgctaatg ttattttgag tgacttctat 7140

ttgcctaata ttgggcctct ccctacgttt gtgggacaga tagttgcgtg gtttaagact 7200

acatttggtg tgtcaaccat ctgtgatttc taccaggtga cggatttggg ctatagaagt 7260

tcgttttgta atggaagtat ggtatgtgaa ctatgcttct caggttttga tatgctggac 7320

aactatgatg ctataaatgt tgttcaacac gttgtagata ggcgtttgtc ctttgactat 7380

attagcctat ttaaactggt agttgagctt gtaatcggct actctcttta tactgtgtgc 7440

ttctacccac tgtttgtcct tattggaatg cagttattga ccacatggtt gcctgaattc 7500

tttatgctgg agactatgca ttggagtgct cgtttgtttg tgtttgttgc caatatgctt 7560

ccagctttta cgttactgcg attttacatc gtggtgacag ctatgtataa ggtctattgt 7620

ctttgtagac atgttatgta tggatgtagt aagcctggtt gcttgttttg ttataagaga 7680

aaccgtagtg tccgtgttaa gtgtagcacc gttgttggtg gttcactacg ctattacgat 7740

gtaatggcta acggcggcac aggtttctgt acaaagcacc agtggaactg tcttaattgc 7800

aattcctgga aaccaggcaa tacattcata actcatgaag cagcggcgga cctctctaag 7860

gagttgaaac gccctgtgaa tccaacagat tctgcttatt actcggtcac agaggttaag 7920

caggttggtt gttccatgcg tttgttctac gagagagatg gacagcgtgt ttatgatgat 7980

gttaatgcta gtttgtttgt ggacatgaat ggtctgctgc attctaaagt taaaggtgtg 8040

cctgaaacgc atgttgtggt tgttgagaat gaagctgata aagctggttt tctcggcgcc 8100

gcagtgtttt atgcacaatc gctctacaga cctatgttga tggtggaaaa gaaattaata 8160

actaccgcca acactggttt gtctgttagt cgaactatgt ttgaccttta tgtagattca 8220

ttgctgaacg tcctcgacgt ggatcgcaag agtctaacaa gttttgtaaa tgctgcgcac 8280

aactctctaa aggagggtgt tcagcttgaa caagttatgg atacctttat tggctgtgcc 8340

cgacgtaagt gtgctataga ttctgatgtt gaaaccaagt ctattaccaa gtccgtcatg 8400

tcggcagtaa atgctggcgt tgattttacg gatgagagtt gtaataactt ggtgcctacc 8460

tatgttaaaa gtgacactat cgttgcagcc gatttgggtg ttcttattca gaataatgct 8520

aagcatgtac aggctaatgt tgctaaagcc gctaatgtgg cttgcatttg gtctgtggat 8580

gcttttaacc agctatctgc tgacttacag cataggctgc gaaaagcatg ttcaaaaact 8640

ggcttgaaga ttaagcttac ttataataag caggaggcaa atgttcctat tttaactaca 8700

ccgttctctc ttaaaggggg cgctgttttt agtagaatgt tacaatggtt gtttgttgct 8760

aatttgattt gtttcattgt gttgtgggcc cttatgccaa catatgcagt gcacaaatcg 8820

gatatgcagt tgcctttata tgccagtttt aaagttatag ataacggtgt gctaagggat 8880

gtgtctgtta ctgacgcatg cttcgcaaac aaatttaatc aattcgacca atggtatgag 8940

tctacttttg gtcttgctta ttaccgcaac tctaaggctt gtcctgttgt ggttgctgta 9000

atagatcaag acattggcca taccttattt aatgttccta ccacagtttt aagatatgga 9060

tttcatgtgt tgcattttat aacccatgca tttgctactg atagcgtgca gtgttacacg 9120

ccacatatgc aaatccccta tgataatttc tatgctagtg gttgcgtgtt gtcatccctc 9180

tgtactatgc ttgcgcatgc agatggaacc ccgcatcctt attgttatac agggggtgtt 9240

atgcataatg cctctctgta tagttctttg gctcctcatg tccgttataa cctggctagt 9300

tcaaatggtt atatacgttt tcccgaagtg gttagtgaag gcattgtgcg tgttgtgcgc 9360

actcgctcta tgacctactg cagggttggt ttatgtgagg aggccgagga gggtatctgc 9420

tttaatttta atcgttcatg ggtattgaac aacccgtatt atagggccat gcctggaact 9480

ttttgtggta ggaatgcttt tgatttaata catcaagttt taggaggatt agtgcggcct 9540

attgatttct ttgccttaac ggcgagttca gtggctggtg ctatccttgc aattattgtc 9600

gttttggctt tctattattt aatcaagctt aagcgtgcct ttggtgacta cactagtgtt 9660

gtggttatca atgtaattgt gtggtgtata aattttctga tgctttttgt gtttcaggtt 9720

tatcccacat tgtcttgttt atatgcttgt ttctacttct acaccacgct ttatttccct 9780

tcggagataa gtgttgttat gcatttgcaa tggcttgtca tgtatggtgc tattatgccc 9840

ttgtggtttt gcattattta cgtggcagtc gttgtttcaa accatgcatt gtggttgttc 9900

tcttactgcc gcaaaattgg taccgaggtt cgtagtgacg gcacatttga ggaaatggcc 9960

cttactacct ttatgattac taaagaatct tattgtaagt tgaaaaactc tgtttctgat 10020

gttgctttta acaggtactt gagtctttac aacaagtacc gttacttcag tggcaaaatg 10080

gatactgccg cttatagaga ggctgcctgt tcacaactgg caaaggcaat ggaaacattt 10140

aaccataata atggtaatga tgttctctat cagcctccaa ccgcctctgt tactacatca 10200

tttttacagt ctggtatagt gaagatggtg tcgcccacct ctaaagtgga gccttgtatt 10260

gttagtgtta cttatggtaa catgacactt aatgggttgt ggttggatga taaagtttat 10320

tgcccaagac atgttatctg ttcttcagct gacatgacag accctgatta tcctaatttg 10380

ctttgtagag tgacatcaag tgatttttgt gttatgtctg gtcgtatgag ccttactgta 10440

atgtcttatc aaatgcaggg ctgccaactt gttttgactg ttacactgca aaatcctaac 10500

acgcctaagt attccttcgg tgttgttaag cctggtgaga catttactgt actggctgca 10560

tacaatggca gacctcaagg agccttccat gttacgcttc gtagtagcca taccataaag 10620

ggctcctttc tatgtggatc ctgcggttct gtaggatatg ttttaactgg cgatagtgta 10680

cgatttgttt atatgcatca gctagagttg agtactggtt gtcataccgg tactgacttt 10740

agtgggaact tttatggtcc ctatagagat gcgcaagttg tacaattgcc tgttcaggat 10800

tatacgcaga ctgttaatgt tgtagcttgg ctttatgctg ctatttttaa cagatgcaac 10860

tggtttgtgc aaagtgatag ttgttccctg gaggagttta atgtttgggc tatgaccaat 10920

ggttttagct caatcaaagc cgatcttgtc ttggatgcgc ttgcttctat gacaggcgtt 10980

acagttgaac aggtgttggc cgctattaag aggctgcatt ctggattcca gggcaaacaa 11040

attttaggta gttgtgtgct tgaagatgag ctgacaccaa gtgatgttta tcaacaacta 11100

gctggtgtca agctacagtc aaagcgcaca agagttataa aaggtacatg ttgctggata 11160

ttggcttcaa cgtttttgtt ctgtagcatt atctcagcat ttgtaaaatg gactatgttt 11220

atgtatgtta ctacccatat gttgggagtg acattgtgtg cactttgttt tgtaagcttt 11280

gctatgttgt tgatcaagca taagcatttg tatttaacta tgtacatcat gcctgtgtta 11340

tgcacactgt tttacaccaa ctatttggtt gtgtacaaac agagttttag aggtctagct 11400

tatgcttggc tttcacactt tgtccctgct gtagattata catatatgga tgaagtttta 11460

tatggtgttg tgttgctagt agctatggtg tttgttacca tgcgtagcat aaaccacgac 11520

gtcttttcta ttatgttctt ggttggtaga cttgtcagcc tggtatccat gtggtatttt 11580

ggagccaatt tagaggaaga ggtactattg ttcctcacat ccctatttgg cacgtacaca 11640

tggactacta tgttgtcatt ggctaccgct aaggttattg ctaaatggtt ggctgtgaat 11700

gtcttgtact tcacagacgt accgcaaatt aaattagttc tgttgagcta cttgtgtatt 11760

ggttatgtgt gttgttgtta ttggggaatc ttgtcactcc ttaatagcat ttttaggatg 11820

ccattgggcg tctacaatta taaaatctcc gttcaggagt tacgttatat gaatgctaat 11880

ggcttgcgcc cacctagaaa tagttttgag gccctgatgc ttaattttaa gctgttggga 11940

attggtggtg tgccagtcat tgaagtatct caaattcaat caagattgac ggatgttaaa 12000

tgtgctaatg ttgtgttgct taattgcctc cagcacttgc atattgcatc taattctaag 12060

ttgtggcagt attgtagtac tttgcacaat gaaatactgg ctacatctga tttgagcgtg 12120

gccttcgata agttggctca actcttagtt gttttatttg ctaatccagc agcagtggat 12180

agcaagtgcc ttgcaagtat tgaagaagtg agcgatgatt acgttcgcga caatactgtc 12240

ttgcaagcct tacagagtga atttgttaat atggctagct tcgttgagta tgaacttgct 12300

aagaagaatc tagatgaggc taaggctagc ggctctgcca atcaacagca gattaagcag 12360

ctagagaagg cgtgtaatat tgctaagtca gcatatgagc gcgacagagc tgttgctcgt 12420

aagctggaac gtatggctga tttagctctt acaaacatgt ataaagaagc tagaattaat 12480

gataagaaga gtaaggtagt gtctgcattg caaaccatgc tctttagtat ggtgcgtaag 12540

ctagataacc aagctcttaa ttctatttta gacaacgcag ttaagggttg tgtacctttg 12600

aatgcaatac catcattgac ttcgaacact ctgactataa tagtgccaga taagcaggtt 12660

tttgatcagg ttgtggataa tgtgtatgtc acctatgctg ggaatgtatg gcatatacag 12720

tttattcaag atgctgatgg tgctgttaaa caattgaatg agatagatgt taattcaacc 12780

tggcctctag tcattgctgc aaataggcat aatgaagtgt ctactgttgt tttgcagaac 12840

aatgagttga tgcctcagaa gttgagaact caggttgtca atagtggctc agatatgaat 12900

tgtaatactc ctacccagtg ttactataat actactggca cgggtaagat tgtgtatgct 12960

atacttagtg actgtgacgg cctgaagtac actaagatag taaaagaaga tggaaattgt 13020

gttgttttgg aattggatcc tccctgtaag ttttctgttc aggatgtgaa gggccttaaa 13080

attaagtacc tttactttgt gaaggggtgt aatacactgg ctagaggctg ggttgtaggc 13140

accttatcct cgacagtgag attgcaggcg ggtacggcaa ctgagtatgc ctccaactct 13200

gcaatactgt cgctgtgtgc gttttctgta gatcctaaga aaacgtactt ggattatata 13260

aaacagggtg gagttcccgt tactaattgt gttaagatgt tatgtgacca tgctggcact 13320

ggtatggcca ttactattaa gccggaggca accactaatc aggattctta tggtggtgct 13380

tccgtttgta tatattgccg ctcgcgtgtt gaacatccag atgttgatgg attgtgcaaa 13440

ttacgcggca agtttgtcca agtgccctta ggcataaaag atcctgtgtc atatgtgttg 13500

acgcatgatg tttgtcaggt ttgtggcttt tggcgagatg gtagctgttc ctgtgtaggc 13560

acaggctccc agtttcagtc aaaagacacg aactttttaa acggattcgg ggtacaagtg 13620

taaatgcccg tcttgtaccc tgtgccagtg gcttggacac tgatgttcaa ttaagggcat 13680

ttgacatttg taatgctaat cgagctggca ttggtttgta ttataaagtg aattgctgcc 13740

gcttccagcg tgtagatgag gacggcaaca agttggataa gttctttgtt gttaaaagaa 13800

ctaatttaga agtgtataac aaggagaaag aatgctatga gttgacaaaa gaatgcggtg 13860

ttgtggctga acacgagttc ttcacatttg atgtggaggg aagtcgggta ccacacatag 13920

tccgtaaaga tctttcaaag tttactatgt tagatctttg ctatgcattg cgtcattttg 13980

accgcaatga ttgttcaact cttaaggaaa ttctccttac atatgctgag tgtgaagagt 14040

cctacttcca aaagaaggac tggtatgatt ttgttgagaa tcctgatata attaatgtgt 14100

acaagaagct tggtcctata tttaatagag ccctgcttaa cactgccaag tttgcagacg 14160

cattagtgga ggcaggctta gtaggtgttt taacacttga taatcaagat ttatatggtc 14220

aatggtatga ctttggagat tttgtcaaga cagtacctgg ttgtggtgtt gccgtggcag 14280

actcttatta ttcatatatg atgccaatgc tgactatgtg tcatgcgttg gatagtgagt 14340

tgtttgttaa tggtacttat agggagtttg accttgttca gtatgatttt actgatttca 14400

agctagagct gttcactaag tattttaagc attggagtat gacctaccac ccgaacacct 14460

gtgagtgcga ggatgacagg tgcattattc attgcgccaa ttttaatata cttttcagca 14520

tggtcttacc taagacctgt tttgggcctc ttgttaggca gatatttgtg gatggtgttc 14580

ctttcgttgt gtcgatcggt taccattata aagaattagg tgttgttatg aatatggatg 14640

tggatacaca tcgttatcgc ttgtctctta aggacttgct tttgtatgct gcagaccctg 14700

cccttcatgt ggcgtctgct agtgcactgc ttgatttgcg cacatgttgt tttagcgttg 14760

cagctattac aagtggcgta aaatttcaaa cagttaaacc tggaaatttt aatcaggatt 14820

tctacgagtt tattttgagt aaaggcctgc ttaaagaggg gagctccgtt gatttgaagc 14880

acttcttctt tacgcaggat ggtaatgctg ctattactga ttacaattac tacaagtata 14940

atctacccac catggtggat attaagcagt tgttgtttgt tttagaagtt gttaataagt 15000

acttcgagat ctatgagggt gggtgtatac ccgcaacaca ggtcattgtt aataattatg 15060

acaagagtgc tggctatcca tttaataaat ttggaaaggc caggctctat tatgaggcat 15120

tatcatttga ggagcaggat gaaatttatg cgtataccaa acgcaatgtc ctgccgaccc 15180

taactcaaat gaatcttaaa tatgctatta gtgctaagaa tagggcccgc accgttgctg 15240

gtgtctctat tctcagtact atgactggca gaatgtttca tcaaaagtgt ctaaagagta 15300

tagcagctac tcgcggtgtt cctgtagtta taggcaccac gaagttctat ggcggttggg 15360

atgatatgtt acgccgcctt attaaagatg ttgatagtcc tgtactcatg ggttgggact 15420

atcctaaatg tgatcgtgct atgccaaaca tactgcgtat tgttagtagt ttggtgctag 15480

cccgtaaaca tgattcgtgc tgttcgcata cggatagatt ctatcgtctt gcgaacgagt 15540

gcgcccaagt tttgagtgaa attgttatgt gtggtggttg ttattatgtt aaaccaggtg 15600

gcactagtag tggggatgca accactgctt ttgctaattc tgtgtttaac atttgtcaag 15660

ctgtttccgc caatgtatgc tcgcttatgg catgcaatgg acacaaaatt gaagatttga 15720

gtatacgcga gttacaaaag cgcctatact ctaatgtcta tcgtgcggac catgttgacc 15780

ccgcatttgt tagtgagtat tatgagtttt taaacaagca ttttagtatg atgattttga 15840

gtgatgatgg tgttgtgtgt tataattcag agtttgcgtc caagggttat attgctaata 15900

taagtgcctt tcaacaggta ttatattatc aaaacaacgt gtttatgtct gaggccaaat 15960

gttgggtaga aacagacatc gaaaagggac cgcatgaatt ttgttctcaa catacaatgc 16020

tagtcaagat ggatggtgat gaagtctacc ttccataccc tgatccttcg agaatcttag 16080

gagcaggctg ttttgttgat gatttactca agactgatag cgttctcttg atagagcgtt 16140

tcgtaagtct tgcaattgat gcttatcctt tagtatacca tgagaaccca gagtatcaaa 16200

atgtgttccg ggtatattta gaatacatca agaagctgta caatgatctc ggtaatcaga 16260

tcctggacag ctacagtgtt attttaagta cttgtgatgg tcaaaagttt actgacgaga 16320

cgttttacaa gaacatgtat ttaagaagtg cagtgctgca aagcgttggt gcctgcgttg 16380

tctgtagttc tcaaacatca ttacgttgtg gcagttgcat acgcaagcct ttgctgtgtt 16440

gcaaatgcgc ctatgatcat gttatgtcca ctgatcataa atatgtcctg agtgtgtcac 16500

catatgtgtg taattcaccg ggatgtgatg taaatgatgt taccaaattg tatttaggtg 16560

gtatgtcata ttattgtgag gaccataaac cacagtattc attcaaattg gtgatgaatg 16620

gtatggtttt tggtttatat aagcagtctt gtactggttc gccctacata gaggatttta 16680

ataaaatcgc tagttgcaaa tggacagaag tcgatgatta tgtgctagct aatgaatgca 16740

ccgaacgcct taaattgttt gccgcagaaa cgcagaaggc cacagaagag gcctttaagc 16800

aatgttatgc gtcagcaacg atccgtgaga tcgtgagcga tcgggagtta attttatctt 16860

gggaaattgg taaagtccgc ccgccactta ataaaaatta cgtgttcacc ggctaccatt 16920

ttactaataa tggtaagaca gttttaggtg agtatgtttt tgataagagt gagttgacta 16980

atggtgtgta ttatcgcgcc acaaccactt ataagttatc tgtaggtgat gtgttcattt 17040

taacatcaca cgcagtgtct agtttaagtg ctcctacatt agtaccgcag gagaattata 17100

ctagcattcg ttttgctagt gtttatagtg tgcctgagac gtttcagaat aatgtgccta 17160

attatcagca cattggaatg aagcgctatt gtactgtaca gggaccgcct ggtactggta 17220

agtcccatct agccattggg ctagctgttt attattgtac agcgcgcgtg gtgtataccg 17280

ctgctagcca tgctgcagtt gacgcgctgt gtgaaaaggc acataaattt ctcaacatca 17340

acgactgcac gcgtattgtt cctgcaaagg tgcgtgtaga ttgttatgat aaattcaagg 17400

tcaatgacac cactcgcaag tatgtgttta ctacaataaa tgcattacct gagttggtga 17460

ctgacattat tgtcgttgat gaagttagta tgcttaccaa ctatgagctg tctgttatta 17520

acagtcgtgt tagggctaag cattatgtgt atattggcga cccggcgcag ttacctgcac 17580

cacgtgtgct actgaataag ggaactctag aacctagata ttttaattcc gttaccaagc 17640

taatgtgttg tttgggtcca gatattttct tgggcacctg ttatagatgc cctaaggaga 17700

ttgtggatac ggtgtcagcc ttggtttata ataataagct gaaggctaaa aatgataata 17760

gctccatgtg ctttaaggtt tattataagg gccagactac acatgagagt tctagtgctg 17820

ttaatatgca gcaaatacat ttaatttcca agtttctgaa ggcaaacccc agttggagta 17880

acgccgtatt tattagtcct tataactcgc agaactatgt tgctaagaga gtcttgggat 17940

tacaaaccca gacagtagac tcagcgcagg gttctgaata tgattttgtt atctactcac 18000

agactgcgga aacagcgcat tctgtcaatg taaatagatt caatgttgct attacacgtg 18060

ctaagaaggg tattctctgt gtcatgagta gtatgcaatt atttgagtct cttaatttta 18120

ctacactgac gttggataag attaacaatc cacgattaca gtgtactaca aatttgttta 18180

aggattgtag caggagctat gtaggatatc acccagccca tgcaccatcc tttttggcag 18240

ttgatgacaa atataaggta ggcggtgatt tagccgtttg ccttaatgtt gctgattctg 18300

ctgtcactta ttcgcggctt atatcactca tgggattcaa gcttgacttg acccttgatg 18360

gttattgtaa gctgtttata actagagatg aagctatcaa acgtgttaga gcctgggttg 18420

gcttcgatgc agaaggtgcc catgcgatac gtgatagcat tgggacaaat ttcccattac 18480

aattaggctt ttcgactgga attgattttg ttgtcgaagc cactggaatg tttgctgaga 18540

gagatggtta tgtctttaaa aaggcagccg cacgagctcc tcctggcgaa caatttaaac 18600

accttatccc acttatgtca agagggcaga aatgggatgt ggttcgcatt agaatagtac 18660

aaatgttgtc agaccaccta gtggatttgg cagacagtgt tgtacttgtg acgtgggctg 18720

ccagctttga gctcacatgt ttgcgatatt tcgctaaagt tggaagagaa gttgtgtgta 18780

gtgtctgcac caagcgtgcg acatgtttta attctagaac tggatactat ggatgctggc 18840

gacatagtta ttcctgtgat tacctgtaca acccactaat agttgacatt caacagtggg 18900

gatatacagg atctttaact agcaatcatg atcctatttg cagcgtgcat aagggtgctc 18960

atgttgcatc atctgatgct atcatgaccc ggtgtctagc tgttcatgat tgcttttgta 19020

agtctgttaa ttggaattta gaatacccca ttatttcaaa tgaggtcagt gttaatacct 19080

cctgcaggtt attgcagcgc gtaatgttta gggctgcgat gctatgcaat aggtatgatg 19140

tgtgttatga cattggcaac cctaaaggtc ttgcctgtgt caaaggatat gattttaagt 19200

tctatgacgc ctcccctgtt gttaagtcgg tcaaacagtt tgtttacaaa tacgaggcac 19260

ataaagatca atttttagat ggtttgtgta tgttttggaa ctgcaatgtg gataagtatc 19320

cagcgaatgc agttgtgtgt aggtttgaca cgcgtgtgtt gaacaaatta aatctccctg 19380

gctgtaatgg tggcagtttg tatgttaaca aacatgcatt ccacaccagt ccctttaccc 19440

gggctgcctt cgagaatttg aagcctatgc ctttctttta ttattcagat acgccctgtg 19500

tgtatatgga aggcatggaa tctaagcagg tcgattatgt cccattgaga agcgctacat 19560

gcatcacaag atgcaattta ggtggcgctg tttgtttaaa acatgctgag gagtatcgtg 19620

agtaccttga gtcttacaat acggcaacca cagcgggttt tactttttgg gtctataaga 19680

cttttgattt ttacaacctt tggaatactt ttactaggct ccaaagttta gaaaatgtag 19740

tgtataacct ggtcaacgct ggacactttg atggccgggc gggtgaactg ccttgtgctg 19800

ttataggtga gaaagtcatt gccaagattc aaaatgagga tgtcgtggtc tttaaaaata 19860

acacgccatt ccccactaat gtggctgtcg aattatttgc taagcgcagt attcggcccc 19920

accccgagct taagctcttt agaaatttga atattgacgt gtgctggagt cacgtccttt 19980

gggattatgc taaggatagt gtgttttgca gttcgacgta taaggtctgc aaatacacag 20040

atttacagtg cattgaaagc ttgaatgtac tttttgatgg tcgtgataat ggtgctcttg 20100

aagcttttaa gaagtgccgg aatggcgtct acattaacac gacaaaaatt aaaagtctgt 20160

cgatgattaa aggcccacaa cgtgccgatt tgaatggcgt agttgtggag aaagttggag 20220

attctgatgt ggaattttgg tttgctgtgc gtaaagacgg tgacgatgtt atcttcagcc 20280

gtacagggag ccttgaaccg agccattacc ggagcccaca aggtaatccg ggtggtaatc 20340

gcgtgggtga tctcagcggt aatgaagctc tagcgcgtgg cactatcttt actcaaagca 20400

gattattatc ttctttcaca cctcgatcag agatggagaa agattttatg gatttagatg 20460

atgatgtgtt cattgcaaaa tatagtttac aggactacgc gtttgaacac gttgtttatg 20520

gtagttttaa ccagaagatt attggaggtt tgcatttgct tattggctta gcccgtaggc 20580

agcaaaaatc caatctggta attcaagagt tcgtgacata cgactctagc attcattcgt 20640

actttatcac tgacgagaac agtggtagta gtaagagtgt gtgcactgtt attgatttat 20700

tgttagatga ttttgtggac attgtaaagt ccctgaatct aaagtgtgtg agtaaggttg 20760

ttaatgttaa tgtggatttt aaggacttcc agtttatgtt gtggtgcaat gaggagaagg 20820

tcatgacttt ctatcctcgt ttgcaggctg ctgctgactg gaaacctggt tatgttatgc 20880

ctgtcttata taagtatttg gaatcgcctc tggaaagagt aaacctctgg aattatggca 20940

agccgattac tttacctaca ggatgtatga tgaatgttgc taagtatact caattatgtc 21000

aatatttgag cactacaaca ttagcagttc cggctaatat gcgtgtctta caccttggtg 21060

ccggttcgga taagggtgtt gcccctgggt ctgcagttct taggcagtgg ctaccagcgg 21120

gaagtattct tgtagataat gatgtgaatc catttgtgag tgacagtgtc gcctcatatt 21180

atggaaattg tataacctta ccctttgatt gtcagtggga tctgataatt tctgatatgt 21240

acgaccctct tactaagaac attggggagt acaacgtgag taaagatgga ttctttactt 21300

acctctgtca tttaattcgt gacaagttgg ctctgggtgg cagtgttgcc ataaaaataa 21360

cagagttttc ttggaacgct gagttatata gtttaatggg gaagtttgcg ttctggacaa 21420

tcttttgcac caacgtaaac gcctcttcaa gtgaaggatt tttgattggc ataaattggt 21480

tgaataagac ccgtaccgaa attgacggta aaaccatgca tgccaattat ctgttttgga 21540

gaaatagtac aatgtggaat ggaggggctt acagtctctt tgacatgagt aagttccctt 21600

tgaaagcggc tggtacggct gttgttagcc ttaaaccaga ccaaataaat gacttagtcc 21660

tctccttgat tgagaagggc aagttattag tgcgtgatac acgcaaagaa gtttttgttg 21720

gcgatagcct agtaaatgtc aaataaacga acaatgtttg tttttcttgt tttattgcca 21780

ctagtctcta gtcagtgtgt taatcttaca accagaactc aattaccccc tgcatacact 21840

aattctttca cacgtggtgt ttattaccct gacaaagttt tcagatcctc agttttacat 21900

tcaactcagg acttgttctt acctttcttt tccaatgtta cttggttcca tgctatacat 21960

gtctctggga ccaatggtac taagaggttt gataaccctg tcctaccatt taatgatggt 22020

gtttactttg cttccactga gaagtctaac ataataagag gctggatttt tggtactact 22080

ttagattcga aaacccagtc cctacttatt gttaataacg ctactaatgt tgttatcaaa 22140

gtctgtgaat ttcaattttg taacgatcca tttttgggtg tttattacca caaaaacaac 22200

aaaagttgga tggaaagtga gttcagagtt tattctagtg cgaataattg cacttttgaa 22260

tacgtctctc agccttttct tatggacctt gaaggaaaac agggtaattt caaaaatctt 22320

agggaatttg tgttcaagaa tattgatggt tacttcaaga tatactctaa gcacacgcct 22380

attaatttag tgcgtgatct ccctcagggt ttttcggctt tagaaccatt ggtagatttg 22440

ccaataggta ttaacatcac taggtttcaa actttacttg ctttacatag aagttattta 22500

actcctggtg attcttcttc aggttggaca gctggtgctg cagcttatta tgtgggttat 22560

cttcaaccta ggacttttct actgaagtac aatgaaaatg gaaccattac agatgctgta 22620

gactgtgcac ttgaccctct ctcagaaaca aagtgtacgt tgaaatcctt cactgtagaa 22680

aaaggaatct atcaaacttc taactttaga gtccaaccaa cagaatctat tgttagattt 22740

cctaacatca caaacttgtg cccttttggt gaagttttta acgccaccag atttgcatct 22800

gtttatgctt ggaacaggaa gagaatcagc aactgtgttg ctgattattc tgtcctgtat 22860

aattccgcat cattttccac ttttaagtgt tatggagtgt ctcctactaa attaaatgat 22920

ctctgcttta ctaatgtcta tgcagattca tttgtaatta gaggtgatga agtcagacaa 22980

atcgctccag ggcaaactgg aaagattgct gattataact acaaattacc agatgatttt 23040

acaggctgcg ttatagcttg gaattctaac aatcttgatt ctaaggttgg tggtaattat 23100

aattacctgt acagattgtt taggaagtct aatctcaaac cttttgagag agatatttca 23160

actgaaatct atcaggccgg tagcacacct tgtaatggtg ttgaaggttt taattgttac 23220

tttcctctgc aatcatatgg tttccaaccc actaatggtg ttggttacca accatacaga 23280

gtagtagtac tttcttttga acttctacat gcaccagcaa ctgtttgtgg acctaaaaag 23340

tctactaatt tggttaagaa caagtgtgtc aatttcaact tcaatggttt aacaggcaca 23400

ggtgttctta ctgagtctaa caaaaagttt ctgcctttcc aacaatttgg cagagacatt 23460

gctgacacta ctgatgctgt tcgtgatcca caaacacttg agattcttga cattacacca 23520

tgttcttttg gtggtgtcag tgttataaca ccaggaacaa atacttctaa ccaggttgct 23580

gttctttatc aggatgttaa ctgcacagaa gtccctgttg ctattcatgc agatcaactt 23640

actcctactt ggcgtgttta ttctacaggt tctaatgttt ttcaaacacg tgcaggctgt 23700

ttaatagggg ctgaacatgt caacaactca tatgagtgtg acatacccat tggtgcaggt 23760

atatgcgcta gttatcagac tcagactaat tctcctcgga gagcaagaag tgtagctagt 23820

caatccatca ttgcctacac tatgtcactt ggtgcagaaa attcagttgc ttactctaat 23880

aactctattg ccatacccac aaattttact attagcgtta ccacagaaat tctaccagtg 23940

tctatgacca agacatcagt agattgtaca atgtacattt gtggtgattc aactgaatgc 24000

agcaatcttt tgttgcaata tggcagtttt tgtacacaat taaaccgtgc tttaactgga 24060

atagctgttg aacaagacaa aaacacccaa gaagtttttg cacaagtcaa acaaatttac 24120

aagacaccac caattaaaga ttttggcggt tttaatttta gccagatact gccagatcca 24180

tcaaaaccaa gcaagaggtc atttattgaa gatctactgt tcaacaaagt gacacttgca 24240

gatgctggct tcatcaaaca atatggtgat tgccttggtg atattgctgc tagagacctc 24300

atttgtgcac aaaagtttaa cggccttact gttttgccac ctttgctcac agatgaaatg 24360

attgctcaat acacttctgc actgttagca ggtacaatca cttctggttg gacttttggt 24420

gcaggtgctg cattacaaat accatttgct atgcaaatgg cttataggtt taatggtatt 24480

ggagttacac agaatgttct ctatgagaac caaaaattga ttgccaacca atttaatagt 24540

gctattggca aaattcaaga ctcactttct tccacagcaa gtgcacttgg aaaacttcaa 24600

gatgtggtca accaaaatgc acaagcttta aacacgcttg ttaaacaact tagctccaat 24660

tttggtgcaa tttcaagtgt tttaaacgac atcctttcac gtcttgacaa agttgaggct 24720

gaagtgcaaa ttgataggtt gatcacaggc agacttcaaa gtttgcagac atatgtgact 24780

caacaattaa ttagagctgc agaaatcaga gcttctgcta atcttgctgc tactaaaatg 24840

tcagagtgtg tacttggaca atcaaaaaga gttgactttt gcggaaaggg ctatcatctt 24900

atgtcatttc ctcagtcagc acctcatggt gtcgtctttt tgcatgtgac ttatgtccct 24960

gcacaagaaa agaacttcac aactgctcct gccatttgtc atgatggaaa agcacacttt 25020

cctcgtgaag gtgtctttgt ttcaaatggc acacactggt ttgtaacaca aaggaatttt 25080

tatgaaccac aaatcattac tacagacaac acatttgtgt ctggtaactg tgatgttgta 25140

ataggaattg tcaacaacac agtttatgat cctttgcaac ctgaattaga ctcattcaag 25200

gaggagcttg ataaatactt caagaaccat acctcaccag atgttgattt aggtgacatc 25260

tctggcatta atgcttcagt tgtaaacatt cagaaagaaa tcgaccgcct caatgaggtt 25320

gccaagaatt taaatgaatc tctcatcgat ctccaagaac ttggaaagta tgagcagtat 25380

ataaaatggc catggtacat ttggctaggt tttatagctg gcttgattgc catagtaatg 25440

gtgacaatta tgctttgctg tatgaccagt tgctgtagtt gtctcaaggg ctgttgttct 25500

tgtggatcct gctgcaaatt tgacgaggac gactctgagc cagtgctcaa aggagtcaaa 25560

ttacattaca cataactatc acagcctctc ctggaaagac agaaaatcta aacaatttat 25620

agcattctca ttgctacctg gccccgtaag aggcagtcat agctatggcc gtgttggtcc 25680

taaggctaca ttggctgctg tctttattgg tccatttatt gtagcatgta tgctaggcat 25740

tggcctagtt tatttattgc aattgcaagt tcaaattttt catgttaagg ataccatacg 25800

tgtgactggc aagccagcca ctgtgtctta tactacaagt acaccagtaa caccgagcgc 25860

gacgacgctc gatggtacta cgtatacttt aattagaccc actagctctt atacaagagt 25920

ttatcttggt actccaagag gttttgatta tagtacattt gggcctaaga ccctagatta 25980

tgttactaat ctaaacctca tcttaattct ggtcgtccat atacttttaa ggcattgtcc 26040

aggcatatga ggccaacagc cacatggatt tggcatgtga gtgatgcatg gttacgccgc 26100

acgcgggact ttggtgtcat tcgcctagaa gatttttgtt ttcaatttaa ttatagccaa 26160

ccccgagttg gttattgtag agttccttta aaggcttggt gtagcaacca gggtaaattt 26220

gcagcgcagt ttaccctaaa aagttgcgaa aaaccaggtc acgaaaaatt tattactagc 26280

ttcacggcct acggcagaac tgtccaacag gccgttagca agttagtaga agaagctgtt 26340

gattttattc tttttagggc cacgcagctc gaaagaaatg tttaatttat tccttacaga 26400

cacagtatgg tatgtggggc agattatttt tatattcgca gtgtgtttga tggtcaccat 26460

aattgtggtt gccttccttg cgtctatcaa actttgtatt caactttgcg gtttatgtaa 26520

tactttggtg ctgtcccctt ctatttattt gtatgatagg agtaagcagc tttataagta 26580

ctataatgaa gaaatgagac tgcccctatt agaggtggat gatatctaat ccaaacatta 26640

tgagtagtac tactcaggcc ccagagcccg tctatcaatg gaccgccgac gaggcagttc 26700

aattccttaa ggaatggaac ttctcgttgg gcattatact actctttatt actatcatac 26760

tacagttcgg ttacacgagc cgtagcatgt ttatttatgt tgtgaaaatg ataatcttgt 26820

ggttaatgtg gccactgact attgttttgt gtattttcaa ttgcgtgtat gcgctaaata 26880

atgtgtatct tggattttct atagtgttta ctatagtgtc cattgtaatc tggatcatgt 26940

attttgtgaa cagcataagg ttgtttatca ggactggtag ctggtggagc ttcaaccccg 27000

aaacaaacaa ccttatgtgt atagatatga aaggtaccgt gtatgttaga cccattattg 27060

aggattacca tacactaaca gccactatta ttcgtggcca cctctacatg caaggtgtta 27120

agctaggcac cggtttctct ttgtctgact tgcccgctta tgttacagtt gctaaggtgt 27180

cacacctttg cacttataag cgcgcattct tagacaaggt agacggtgtt agcggttttg 27240

ctgtttatgt gaagtccaag gtcggaaatt accgactgcc ctcaaacaaa ccgagtggcg 27300

cggacaccgc attgttgaga acctaatcta aactttaagg agagaatgaa tcctatgtcg 27360

gcgctcggtg gtaacccctc gcgagaaagt cgggatagga cactctctat cagaatggat 27420

gtcttgctgt cataacagat agagaaggtt gtggcagacc ctgtatcaat tagttgaaag 27480

agattgcaaa atagagaatg tgtgagagaa gttagcaagg tcctacgtct aaccataaga 27540

acggcgatag gcgccccctg ggaacagctc acatcagggt actattcctg caatgcccta 27600

gtaaatgaat gaagttgatc atggccaatt ggaagaatca caaaaaaaaa aaaaaaaaaa 27660

acggccggtt t 27671

<210> 35

<211> 7341

<212> DNA

<213> Artificial sequence

<220>

<223> pcDNA34_syn_N

<400> 35

agtacttaat acgactcact ataggctagc cgccaccatg gtgtctgata atggacctca 60

aaatcagcga aatgcacctc gcattacgtt tggtggacca tcagattcaa ctggcagtaa 120

ccagaatgga gaacgaagtg gtgcgcgatc aaaacaacgc cgcccgcaag gtttacccaa 180

taatactgcg tcttggttca ccgctctcac tcaacatggc aaggaagatt taaaattccc 240

tcgaggacaa ggcgttccaa ttaacaccaa tagcagtcca gatgaccaaa ttggctacta 300

ccgccgcgcc acaagacgaa ttcgtggtgg tgatggtaaa atgaaagatc tcagtccaag 360

atggtatttc tactatctag gaactgggcc agaagctgga cttccttatg gtgctaacaa 420

agatggcatc atatgggttg caactgaggg agccttgaat acaccaaaag atcacattgg 480

caccagaaat cctgctaaca atgctgcaat cgtgctacaa cttcctcaag gaacaacatt 540

accaaaaggt ttttacgcag aagggtctag aggtggaagt caagcctctt ctagatcatc 600

atcacgtagt cgcaacagtt caagaaattc aactccaggt tcaagtagag gaacttctcc 660

tgctagaatg gctggaaatg gaggtgatgc tgctcttgct ttgttactac ttgacagatt 720

gaaccagctt gagagcaaaa tgtctggtaa aggccaacaa caacaaggcc aaactgtcac 780

taagaaatct gctgctgagg cttctaagaa gcctagacaa aaacgtactg ccactaaagc 840

atacaatgta acacaagctt tcggcagacg tggtccagaa caaactcaag gaaattttgg 900

ggatcaggaa ctaatcagac aaggaactga ttacaaacat tggccgcaaa ttgcacaatt 960

tgctccttct gcttcagcgt tctttggaat gtcgagaatt ggaatggaag tcacaccttc 1020

gggaacatgg ttgacctata caggtgccat caaattggat gacaaagatc caaatttcaa 1080

agatcaagtc attttgctga ataagcatat tgacgcatac aaaacattcc caccaacaga 1140

gcctaaaaag gacaaaaaga agaaggctga tgaaactcaa gccttaccgc agagacagaa 1200

gaaacagcaa actgtgactc ttcttcctgc tgcagatttg gatgatttct ccaaacaatt 1260

gcaacaatcc atgagcagtg ctgactcaac tcaggcctaa gcggccgctt cgagcagaca 1320

tgataagata aagggttcga tccctaccgg ttagtaatga gtttgatatc tcgacaatca 1380

acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 1440

tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 1500

tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 1560

cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 1620

gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 1680

cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 1740

cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 1800

tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 1860

agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 1920

tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa acgggggagg 1980

ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 2040

agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca 2100

gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc 2160

ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt 2220

cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca 2280

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 2340

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 2400

gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 2460

tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 2520

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 2580

actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 2640

agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 2700

cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 2760

gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 2820

ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 2880

gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 2940

ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 3000

ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 3060

ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 3120

attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3180

tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3240

caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 3300

gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 3360

gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 3420

ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 3480

cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 3540

gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 3600

catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 3660

gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 3720

cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 3780

gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 3840

gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 3900

gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct 3960

gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 4020

tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 4080

ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4140

tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 4200

tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 4260

atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 4320

aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 4380

gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 4440

ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 4500

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4560

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4620

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4680

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4740

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4800

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 4860

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4920

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4980

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 5040

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 5100

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 5160

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 5220

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5280

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5340

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5400

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5460

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 5520

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 5580

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 5640

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 5700

agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 5760

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 5820

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 5880

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5940

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 6000

tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 6060

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 6120

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 6180

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 6240

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 6300

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 6360

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 6420

atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca 6480

tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 6540

aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 6600

gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 6660

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 6720

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 6780

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 6840

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 6900

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 6960

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 7020

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 7080

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 7140

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 7200

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 7260

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 7320

actctagagg atcgaaccct t 7341

<210> 36

<211> 6309

<212> DNA

<213> Artificial sequence

<220>

<223> pcDNA34_syn_E

<400> 36

agtacttaat acgactcact ataggctagc cgccaccatg gtgtactcat tcgtttcgga 60

agagacaggt acgttaatag ttaatagcgt acttcttttt cttgctttcg tggtattctt 120

gctagttaca ctagccattc ttactgcgct tcgattgtgt gcgtactgtt gcaatattgt 180

taacgtgagt cttgtaaaac cttcttttta cgtttactct cgtgttaaaa atctgaattc 240

ttctcgggtt cctgatcttc tggtctaagc ggccgcttcg agcagacatg ataagataaa 300

gggttcgatc cctaccggtt agtaatgagt ttgatatctc gacaatcaac ctctggatta 360

caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 420

atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 480

ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 540

acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 600

cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 660

catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 720

cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 780

gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 840

ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 900

gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca 960

cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 1020

acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 1080

tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 1140

accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 1200

ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag 1260

cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 1320

cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 1380

tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 1440

cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 1500

gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 1560

aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 1620

gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 1680

ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 1740

atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 1800

gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 1860

actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 1920

ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 1980

tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 2040

tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 2100

ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 2160

caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 2220

gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 2280

cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 2340

gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 2400

caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 2460

cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 2520

actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 2580

gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 2640

gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 2700

ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 2760

cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 2820

atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 2880

gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 2940

tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 3000

cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 3060

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 3120

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 3180

tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 3240

tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 3300

taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 3360

cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 3420

gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 3480

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 3540

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3600

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3660

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3720

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3780

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 3840

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3900

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3960

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4020

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4080

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 4140

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4200

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4260

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 4320

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 4380

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4440

atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4500

tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4560

aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4620

catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4680

gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4740

ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4800

aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4860

atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4920

cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 4980

gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 5040

agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5100

gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5160

caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 5220

ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 5280

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5340

aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 5400

cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag 5460

tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 5520

acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt 5580

gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat 5640

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 5700

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 5760

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 5820

gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 5880

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 5940

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 6000

atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 6060

agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 6120

ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 6180

gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 6240

cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat 6300

cgaaccctt 6309

<210> 37

<211> 6750

<212> DNA

<213> Artificial sequence

<220>

<223> pcDNA34_syn_M

<400> 37

agtacttaat acgactcact ataggctagc cgccaccatg gtggcagatt ccaacggtac 60

tattaccgtt gaggagctga aaaagctcct tgaacaatgg aacctagtaa taggtttcct 120

attccttaca tggatttgcc tgctgcaatt tgcctatgcc aacaggaata ggtttttgta 180

catcattaag ttgattttcc tctggctgtt atggccagta actttagctt gttttgtgct 240

tgctgctgtt tacagaataa attggatcac cggtggaatt gctattgcaa tggcttgtct 300

tgtaggattg atgtggctaa gctacttcat tgcttctttc agactgtttg cgcgtacgcg 360

ttccatgtgg tcattcaatc cagaaactaa cattcttctc aacgtgccac tccatggaac 420

tattctgact agaccgcttc tagaaagtga actcgtaatc ggagctgtta tccttcgtgg 480

acatcttcgt attgctggac atcatctagg acgctgtgac atcaaggatc tacctaaaga 540

aatcactgtt gctacatcac gaacgctttc ttattacaaa ttgggagctt cacagcgtgt 600

agcaggtgat tcaggttttg ctgcatatag tcgctacagg attggcaact ataaattaaa 660

cacagaccat tccagtagca gtgacaatat tgctttgctt gtacagtaag cggccgcttc 720

gagcagacat gataagataa agggttcgat ccctaccggt tagtaatgag tttgatatct 780

cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 840

tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 900

ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 960

gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 1020

cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 1080

ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 1140

gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 1200

gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 1260

cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 1320

tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaaa 1380

cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 1440

gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt 1500

tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc 1560

ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 1620

agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg gctctagggg 1680

gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 1740

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 1800

tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt 1860

ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 1920

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 1980

taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 2040

tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca 2100

aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2160

ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 2220

ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2280

gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 2340

cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 2400

gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 2460

aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg 2520

tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 2580

ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 2640

ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 2700

gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 2760

gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 2820

gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 2880

gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 2940

catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 3000

gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg 3060

cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 3120

gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 3180

caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 3240

cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 3300

cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac 3360

gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3420

cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 3480

gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3540

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3600

actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 3660

atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3720

acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 3780

aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3840

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 3900

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3960

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 4020

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4080

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 4140

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4200

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4260

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 4320

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4380

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4440

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4500

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4560

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4620

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4680

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4740

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4800

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4860

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4920

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4980

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 5040

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 5100

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 5160

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 5220

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 5280

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 5340

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5400

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5460

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5520

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5580

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5640

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5700

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5760

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5820

cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct 5880

gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 5940

tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 6000

ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga 6060

cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 6120

tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 6180

gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 6240

ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 6300

gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 6360

cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 6420

gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 6480

tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 6540

caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 6600

ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag 6660

atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc 6720

agcctccgga ctctagagga tcgaaccctt 6750

<210> 38

<211> 9905

<212> DNA

<213> Artificial sequence

<220>

<223> pcDNA34_syn_S

<400> 38

agtacttaat acgactcact ataggctagc gccgccacca tggtgtttgt ttttcttgtt 60

ttattgccac tagtctctag tcagtgtgtt aatcttacaa ccagaactca attaccccct 120

gcatacacta attctttcac acgtggtgtt tattaccctg acaaagtttt cagatcctca 180

gttttacatt caactcagga cttgttctta cctttctttt ccaatgttac ttggttccat 240

gctatacatg tctctgggac caatggtact aagaggtttg ataaccctgt cctaccattt 300

aatgatggtg tttactttgc ttccactgag aagtctaaca taataagagg ctggattttt 360

ggtactactt tagattcgaa aacccagtcc ctacttattg ttaataacgc tactaatgtt 420

gttatcaaag tctgtgaatt tcaattttgt aacgatccat ttttgggtgt ttattaccac 480

aaaaacaaca aaagttggat ggaaagtgag ttcagagttt attctagtgc gaataattgc 540

acttttgaat acgtctctca gccttttctt atggaccttg aaggaaaaca gggtaatttc 600

aaaaatctta gggaatttgt gttcaagaat attgatggtt acttcaagat atactctaag 660

cacacgccta ttaatttagt gcgtgatctc cctcagggtt tttcggcttt agaaccattg 720

gtagatttgc caataggtat taacatcact aggtttcaaa ctttacttgc tttacataga 780

agttatttaa ctcctggtga ttcttcttca ggttggacag ctggtgctgc agcttattat 840

gtgggttatc ttcaacctag gacttttcta ctgaagtaca atgaaaatgg aaccattaca 900

gatgctgtag actgtgcact tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc 960

actgtagaaa aaggaatcta tcaaacttct aactttagag tccaaccaac agaatctatt 1020

gttagatttc ctaacatcac aaacttgtgc ccttttggtg aagtttttaa cgccaccaga 1080

tttgcatctg tttatgcttg gaacaggaag agaatcagca actgtgttgc tgattattct 1140

gtcctgtata attccgcatc attttccact tttaagtgtt atggagtgtc tcctactaaa 1200

ttaaatgatc tctgctttac taatgtctat gcagattcat ttgtaattag aggtgatgaa 1260

gtcagacaaa tcgctccagg gcaaactgga aagattgctg attataacta caaattacca 1320

gatgatttta caggctgcgt tatagcttgg aattctaaca atcttgattc taaggttggt 1380

ggtaattata attacctgta cagattgttt aggaagtcta atctcaaacc ttttgagaga 1440

gatatttcaa ctgaaatcta tcaggccggt agcacacctt gtaatggtgt tgaaggtttt 1500

aattgttact ttcctctgca atcatatggt ttccaaccca ctaatggtgt tggttaccaa 1560

ccatacagag tagtagtact ttcttttgaa cttctacatg caccagcaac tgtttgtgga 1620

cctaaaaagt ctactaattt ggttaagaac aagtgtgtca atttcaactt caatggttta 1680

acaggcacag gtgttcttac tgagtctaac aaaaagtttc tgcctttcca acaatttggc 1740

agagacattg ctgacactac tgatgctgtt cgtgatccac aaacacttga gattcttgac 1800

attacaccat gttcttttgg tggtgtcagt gttataacac caggaacaaa tacttctaac 1860

caggttgctg ttctttatca ggatgttaac tgcacagaag tccctgttgc tattcatgca 1920

gatcaactta ctcctacttg gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt 1980

gcaggctgtt taataggggc tgaacatgtc aacaactcat atgagtgtga catacccatt 2040

ggtgcaggta tatgcgctag ttatcagact cagactaatt ctcctcggag agcaagaagt 2100

gtagctagtc aatccatcat tgcctacact atgtcacttg gtgcagaaaa ttcagttgct 2160

tactctaata actctattgc catacccaca aattttacta ttagcgttac cacagaaatt 2220

ctaccagtgt ctatgaccaa gacatcagta gattgtacaa tgtacatttg tggtgattca 2280

actgaatgca gcaatctttt gttgcaatat ggcagttttt gtacacaatt aaaccgtgct 2340

ttaactggaa tagctgttga acaagacaaa aacacccaag aagtttttgc acaagtcaaa 2400

caaatttaca agacaccacc aattaaagat tttggcggtt ttaattttag ccagatactg 2460

ccagatccat caaaaccaag caagaggtca tttattgaag atctactgtt caacaaagtg 2520

acacttgcag atgctggctt catcaaacaa tatggtgatt gccttggtga tattgctgct 2580

agagacctca tttgtgcaca aaagtttaac ggccttactg ttttgccacc tttgctcaca 2640

gatgaaatga ttgctcaata cacttctgca ctgttagcag gtacaatcac ttctggttgg 2700

acttttggtg caggtgctgc attacaaata ccatttgcta tgcaaatggc ttataggttt 2760

aatggtattg gagttacaca gaatgttctc tatgagaacc aaaaattgat tgccaaccaa 2820

tttaatagtg ctattggcaa aattcaagac tcactttctt ccacagcaag tgcacttgga 2880

aaacttcaag atgtggtcaa ccaaaatgca caagctttaa acacgcttgt taaacaactt 2940

agctccaatt ttggtgcaat ttcaagtgtt ttaaacgaca tcctttcacg tcttgacaaa 3000

gttgaggctg aagtgcaaat tgataggttg atcacaggca gacttcaaag tttgcagaca 3060

tatgtgactc aacaattaat tagagctgca gaaatcagag cttctgctaa tcttgctgct 3120

actaaaatgt cagagtgtgt acttggacaa tcaaaaagag ttgacttttg cggaaagggc 3180

tatcatctta tgtcatttcc tcagtcagca cctcatggtg tcgtcttttt gcatgtgact 3240

tatgtccctg cacaagaaaa gaacttcaca actgctcctg ccatttgtca tgatggaaaa 3300

gcacactttc ctcgtgaagg tgtctttgtt tcaaatggca cacactggtt tgtaacacaa 3360

aggaattttt atgaaccaca aatcattact acagacaaca catttgtgtc tggtaactgt 3420

gatgttgtaa taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac 3480

tcattcaagg aggagcttga taaatacttc aagaaccata cctcaccaga tgttgattta 3540

ggtgacatct ctggcattaa tgcttcagtt gtaaacattc agaaagaaat cgaccgcctc 3600

aatgaggttg ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat 3660

gagcagtata taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc 3720

atagtaatgg tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc 3780

tgttgttctt gtggatcctg ctgcaaattt gacgaggacg actctgagcc agtgctcaaa 3840

ggagtcaaat tacattacac ataagcggcc gcttcgagca gacatgataa gataaagggt 3900

tcgatcccta ccggttagta atgagtttga tatctcgaca atcaacctct ggattacaaa 3960

atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 4020

gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 4080

ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 4140

ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 4200

tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 4260

gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 4320

gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 4380

ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 4440

cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 4500

cggatctccc tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga 4560

aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc 4620

acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc 4680

gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc 4740

caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct 4800

gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 4860

gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 4920

ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 4980

cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc 5040

gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 5100

gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 5160

ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt 5220

tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 5280

ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 5340

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 5400

gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 5460

cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 5520

ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 5580

gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 5640

ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 5700

tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 5760

agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 5820

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 5880

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 5940

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 6000

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 6060

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 6120

ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 6180

cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 6240

cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 6300

tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 6360

atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 6420

ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 6480

gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 6540

gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 6600

tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 6660

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 6720

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 6780

tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 6840

tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 6900

gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 6960

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7020

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7080

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7140

tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7200

aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7260

aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7320

ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7380

tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 7440

agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7500

gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 7560

tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 7620

acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 7680

tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 7740

caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 7800

aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 7860

aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 7920

ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 7980

agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 8040

atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 8100

cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 8160

aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 8220

cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 8280

aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 8340

ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 8400

gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 8460

ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8520

tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 8580

tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 8640

ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 8700

tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8760

agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 8820

acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 8880

ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 8940

gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg 9000

atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 9060

tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 9120

caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 9180

tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat 9240

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 9300

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 9360

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 9420

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 9480

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 9540

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 9600

ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 9660

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 9720

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 9780

tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct 9840

gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gaggatcgaa 9900

ccctt 9905

<210> 39

<211> 40556

<212> DNA

<213> Artificial sequence

<220>

<223> pMR10Y_COVAX191_delN

<400> 39

atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc 60

gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc 120

cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa 180

ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtctcgaa 240

caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc 300

aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc 360

gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt 420

gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc 480

accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc 540

aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg 600

gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc 660

acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag 720

gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt 780

gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa 840

acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac 900

tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc 960

gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg 1020

tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa 1080

gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc 1140

aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagccac tcgatcgagg 1200

tcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 1260

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 1320

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 1380

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttccat 1440

gggatatcga gatctcctgc agagctctag agtcgagact agtctcgacg ggcccggtac 1500

cccctcgagg gggccgcact taagttacgc gtggatcgtg gagctttcgg gttttaacta 1560

taacggtcct aaggtagcga actcgggtct tgccttaatc ccaacaaccg gattatctac 1620

acggatttca atagctgata tagcgaatca ccgagattaa ttaataatac gactcactat 1680

agtataagag tgattggcgt ccgtacgtac cctctcaact ctaaaactct tgtagtttaa 1740

atctaatcta aactttataa acggcacttc ctgcgtgtcc atgcccgcgg gcctggtctt 1800

gtcatagtgc tgacatttgt agttccttga ctttcgttct ctgccagtga cgtgtccatt 1860

cggcgccagc agcccaccca taggttgcat aatggcaaag atgggcaaat acggcctggg 1920

cttcaaatgg gccccagaat ttccatggat gcttccgaac gcatcggaga agttgggtaa 1980

ccctgagagg tcagaggagg atgggttttg cccctctgct gcgcaagaac cgaaagttaa 2040

aggaaaaact ttggttaatc acgtgagggt gaattgtagc cggcttccag ctttggaatg 2100

ctgtgttcag tctgccataa tccgtgatat ttttgtagat gaggatcccc agaaggtgga 2160

ggcctcaact atgatggcat tgcagttcgg tagtgccgtc ttggttaagc catccaagcg 2220

cttgtctatt caggcatgga ctaatttggg tgtgcttccc aaaacagctg ccatggggtt 2280

gttcaagcgc gtctgcctgt gtaacaccag ggagtgctct tgtgacgccc acgtggcctt 2340

tcaccttttt acggtccaac ccgatggtgt atgcctgggt aatggccgtt ttataggctg 2400

gttcgttcca gtcacagcca taccggagta tgcgaagcag tggttgcaac cctggtccat 2460

ccttcttcgt aagggtggta acaaagggtc tgtgacatcc ggccacttcc gccgcgctgt 2520

taccatgcct gtgtatgact ttaatgtaga ggatgcttgt gaggaggttc atcttaaccc 2580

gaagggtaag tactcctgca aggcgtatgc cctgctgaag ggctatcgcg gtgttaagcc 2640

catcctgttt gtggaccagt atggttgcga ctatactgga tgtctcgcca agggtcttga 2700

ggactatggc gatctcacct tgagtgagat gaaggagttg ttccctgtgt ggcgtgactc 2760

cttggatagt gaagtccttg tggcttggca cgttgatcga gatcctcggg ctgctatgcg 2820

tctgcagact cttgctactg tacgttgcat tgattatgtg ggccaaccga ccgaggatgt 2880

ggtggatgga gatgtggtag tgcgtgagcc tgctcatctt ctcgcagcca atgccattgt 2940

taaaagactc ccccgtttgg tggagactat gctgtatacg gattcgtccg ttacagaatt 3000

ctgttataaa accaagctgt gtgaatgcgg ttttatcacg cagtttggct atgtggattg 3060

ttgtggtgac acctgtgatt ttcgtgggtg ggttgccggc aatatgatgg atggctttcc 3120

atgtccaggg tgtaccaaaa attatatgcc ctgggaattg gaggcccagt catcaggtgt 3180

tataccagaa ggaggtgttc tattcactca gagcactgat acagtgaatc gtgagtcctt 3240

taagctctac ggtcatgctg ttgtgccttt tggttctgct gtgtattgga gcccttgccc 3300

aggtatgtgg cttccagtaa tttggtcgtc ggttaagtca tactctggtt tgacttatac 3360

aggagtagtt ggttgtaagg caattgttca agagacagac gctatatgtc gttctctgta 3420

tatggattat gtccagcaca agtgtggcaa tctcgagcag agagctatcc ttggattgga 3480

cgatgtctat catagacagt tgcttgtgaa taggggtgac tatagtctcc tccttgagaa 3540

tgtggatttg tttgttaagc ggcgcgctga atttgcttgc aaattcgcca cctgtggaga 3600

tggtcttgta cccctcctac tagatggttt agtgccccgc agttattatt tgattaagag 3660

tggtcaagct ttcacctcta tgatggttaa ttttagccat gaggtgactg acatgtgtat 3720

ggacatggct ttattgttca tgcatgatgt taaagtggcc actaagtatg ttaagaaggt 3780

tactggcaaa ctggccgtgc gctttaaagc gttgggtgta gccgttgtca gaaaaattac 3840

tgaatggttt gatttagccg tggacattgc tgctagtgcc gctggatggc tttgctacca 3900

gctggtaaat ggcttatttg cagtggccaa tggtgttata acctttgtac aggaggtgcc 3960

tgagcttgtc aagaattttg ttgacaagtt caaggcattt ttcaaggttt tgatcgactc 4020

tatgtcggtt tctatcttgt ctggacttac tgttgtcaag actgcctcaa atagggtgtg 4080

tcttgctggc agtaaggttt atgaagttgt gcagaaatct ttgtctgcat atgttatgcc 4140

tgtgggttgc agcgaagcca cttgtttggt gggtgagatt gaacctgcag tttttgaaga 4200

tgatgttgtt gatgtggtta aagccccatt aacatatcaa ggctgttgta agccacccac 4260

ttctttcgag aagatttgta ttgtggataa attgtatatg gccaagtgtg gtgatcaatt 4320

ttaccctgtg gttgttgata acgacactgt tggcgtgtta gatcagtgct ggaggtttcc 4380

ctgtgcgggc aagaaagtcg agtttaacga caagcccaaa gtcaggaaga taccctccac 4440

ccgtaagatt aagatcacct tcgcactgga tgcgaccttt gatagtgttc tttcgaaggc 4500

gtgttcagag tttgaagttg ataaagatgt tacattggat gagctgcttg atgttgtgct 4560

tgacgcagtt gagagtacgc tcagcccttg taaggagcat gatgtgatag gcacaaaagt 4620

ttgtgcttta cttgataggt tggcaggaga ttatgtctat ctttttgatg agggaggcga 4680

tgaagtgatc gccccgagga tgtattgttc cttttctgct cctgatgacg aggactgcgt 4740

tgcagcggat gttgtagatg cagatgaaaa ccaagatgat gatgccgagg actcagcagt 4800

ccttgtcgct gatacccaag aagaggacgg cgttgccaag gggcaggttg aggcggattc 4860

ggaaatttgc gttgcgcata ctggtagtca agaagaattg gctgagcctg atgctgtcgg 4920

atctcaaact cccatcgcct ctgctgagga aaccgaagtc ggagaggcaa gcgacaggga 4980

agggattgct gaggcgaagg caactgtgtg tgctgatgct gtagatgcct gccccgatca 5040

agtggaggca tttgaaattg aaaaggtcga ggactctatc ttggatgagc ttcaaactga 5100

acttaatgcg ccagcggaca agacctatga ggatgtcttg gcattcgatg ccgtatgctc 5160

agaggcgttg tctgcattct atgctgtgcc gagtgatgag acgcacttta aagtgtgtgg 5220

attctattcg cctgctatag agcgcactaa ttgttggctg cgttctactt tgatagtaat 5280

gcagagtcta cctttggaat ttaaagactt ggagatgcaa aagctctggt tgtcttacaa 5340

ggccggctat gaccaatgct ttgtggacaa actagttaag agcgtgccca agtctattat 5400

ccttccacaa ggtggttatg tggcagattt tgcctatttc tttctaagcc agtgtagctt 5460

taaagcttat gctaactggc gttgtttaga gtgtgacatg gagttaaagc ttcaaggctt 5520

ggacgccatg tttttctatg gggacgttgt gtctcatatg tgcaagtgtg gtaatagcat 5580

gaccttgttg tctgcagata taccctacac tttgcatttt ggagtgcgag atgataagtt 5640

ttgcgctttt tacacgccaa gaaaggtctt tagggctgct tgtgcggtag atgttaatga 5700

ttgtcactct atggctgtag tagagggcaa gcaaattgat ggtaaagtgg ttaccaaatt 5760

tattggtgac aaatttgatt ttatggtggg ttacgggatg acatttagta tgtctccttt 5820

tgaactcgcc cagttatatg gttcatgtat aacaccaaat gtttgttttg ttaaaggaga 5880

tgttataaag gttgttcgct tagttaatgc tgaagtcatt gttaaccctg ctaatgggcg 5940

tatggctcat ggtgccggcg tcgccggcgc catagctgaa aaggcgggca gtgcttttat 6000

taaagaaacc tccgatatgg tgaaggctca gggcgtttgc caggttggtg aatgctatga 6060

atctgccggt ggtaagttat gtaaaaaggt gcttaacatt gtagggccag atgcgcgagg 6120

gcatggcaag caatgctatt cacttttaga gcgtgcttat cagcatatta ataagtgtga 6180

caatgttgtc actactttaa tttcggctgg tatatttagt gtgcctactg atgtctccct 6240

aacttactta cttggtgtag tgacaaagaa tgtcattctt gtcagtaaca accaggatga 6300

ttttgatgtg atagagaagt gtcaggtgac ctccgttgct ggtaccaaag cgctatcact 6360

tcaattggcc aaaaatttgt gccgtgatgt aaagtttgtg acgaatgcat gtagttcgct 6420

ttttagtgaa tcttgctttg tctcaagcta tgatgtgttg caggaagttg aagcgctgcg 6480

acatgatata caattggatg atgatgctcg tgtctttgtg caggctaata tggactgtct 6540

gcccacagac tggcgtctcg ttaacaaatt tgatagtgtt gatggtgtta gaaccattaa 6600

gtattttgaa tgcccgggcg ggatttttgt atccagccag ggcaaaaagt ttggttatgt 6660

tcagaatggt tcatttaagg aggcgagtgt tagccaaata agggctttac tcgctaataa 6720

ggttgatgtc ttgtgtactg ttgatggtgt taacttccgc tcctgctgcg tagcagaggg 6780

tgaagttttt ggcaagacat taggttcagt cttttgtgat ggcataaatg tcaccaaagt 6840

taggtgtagt gccatttaca agggtaaggt tttctttcag tacagtgatt tgtccgaggc 6900

agatcttgtg gctgttaaag atgcctttgg ttttgatgaa ccacaactgc tgaagtacta 6960

cactatgctt ggcatgtgta agtggccagt agttgtttgt ggcaattatt ttgctttcaa 7020

gcagtcaaat aataattgct acatcaacgt ggcatgttta atgctgcaac acttgagttt 7080

aaagtttcct aagtggcaat ggcaagaggc ttggaacgag ttccgctctg gtaaaccact 7140

aaggtttgtg tccttggtat tagcaaaggg cagctttaaa tttaatgaac cttctgattc 7200

tatcgatttt atgcgtgtgg tgctacgtga agcagatttg agtggtgcca cgtgcaattt 7260

ggaatttgtt tgtaaatgtg gtgtgaagca agagcagcgc aaaggtgttg acgctgttat 7320

gcattttggt acgttggata aaggtgatct tgtcaggggt tataatatcg catgtacgtg 7380

cggtagtaaa cttgtgcatt gcacccaatt taacgtacca tttttaattt gctccaacac 7440

accagagggt aggaaactgc ccgacgatgt tgttgcagct aatattttta ctggtggtag 7500

tgtgggccat tacacgcatg tgaaatgtaa acccaagtac cagctttatg atgcttgtaa 7560

tgttaataag gtttcggagg ctaagggtaa ttttaccgat tgcctctacc ttaaaaattt 7620

aaagcaaacc ttctcgtctg tgctgacgac tttttattta gatgacgtaa agtgtgtgga 7680

gtataagcca gatttatcgc agtattactg tgagtctggt aaatattata caaaacccat 7740

tattaaggcc caatttagaa catttgagaa ggttgatggt gtctatacca actttaaatt 7800

ggtgggacat agtattgctg aaaaactcaa tgctaagctg ggatttgatt gtaattctcc 7860

ctttgtggag tataaaatta cagagtggcc aacagctact ggagatgtgg tgttggctag 7920

tgatgatttg tatgtaagtc ggtacttaag cgggtgcatt acttttggta aaccggttgt 7980

ctggcttggc catgaggaag catcgctgaa atctctcaca tattttaata gacctagtgt 8040

cgtttgtgaa aataaattta acgtgttgcc cgttgatgtc agtgaaccca cggacaaggg 8100

gcctgtgcct gctgcagtcc ttgttaccgg cgtccctgga gctgatgcgt cagctggtgc 8160

cggtattgcc aaggagcaaa aagcctgtgc ttctgctagt gtggaggatc aggttgttac 8220

ggaggttcgt caagagccat ctgtttcagc tgctgatgtc aaagaggtta aattgaatgg 8280

tgttaaaaag cctgttaagg tggaaggtag tgtggttgtt aatgatccca ctagcgaaac 8340

caaagttgtt aaaagtttgt ctattgttga tgtctatgat atgttcctga cagggtgtaa 8400

gtatgtggtt tggactgcta atgagttgtc tcgactagta aattcaccga ctgttaggga 8460

gtatgtgaag tggggtatgg gaaagattgt aacacccgct aagttgttgt tgttaagaga 8520

tgagaagcaa gagttcgtag cgccaaaagt agtcaaggcg aaagctattg cctgctattg 8580

tgctgtgaag tggtttctcc tctattgttt tagttggata aagtttaata ctgacaataa 8640

ggttatatac accacagaag tagcttcaaa gcttactttc aagttgtgct gtttggcctt 8700

taagaatgcc ttacagacgt ttaattggag cgttgtgtct aggggctttt tcctagttgc 8760

aacggtcttt ttactctggt ttaacttttt gtatgctaat gttattttga gtgacttcta 8820

tttgcctaat attgggcctc tccctacgtt tgtgggacag atagttgcgt ggtttaagac 8880

tacatttggt gtgtcaacca tctgtgattt ctaccaggtg acggatttgg gctatagaag 8940

ttcgttttgt aatggaagta tggtatgtga actatgcttc tcaggttttg atatgctgga 9000

caactatgat gctataaatg ttgttcaaca cgttgtagat aggcgtttgt cctttgacta 9060

tattagccta tttaaactgg tagttgagct tgtaatcggc tactctcttt atactgtgtg 9120

cttctaccca ctgtttgtcc ttattggaat gcagttattg accacatggt tgcctgaatt 9180

ctttatgctg gagactatgc attggagtgc tcgtttgttt gtgtttgttg ccaatatgct 9240

tccagctttt acgttactgc gattttacat cgtggtgaca gctatgtata aggtctattg 9300

tctttgtaga catgttatgt atggatgtag taagcctggt tgcttgtttt gttataagag 9360

aaaccgtagt gtccgtgtta agtgtagcac cgttgttggt ggttcactac gctattacga 9420

tgtaatggct aacggcggca caggtttctg tacaaagcac cagtggaact gtcttaattg 9480

caattcctgg aaaccaggca atacattcat aactcatgaa gcagcggcgg acctctctaa 9540

ggagttgaaa cgccctgtga atccaacaga ttctgcttat tactcggtca cagaggttaa 9600

gcaggttggt tgttccatgc gtttgttcta cgagagagat ggacagcgtg tttatgatga 9660

tgttaatgct agtttgtttg tggacatgaa tggtctgctg cattctaaag ttaaaggtgt 9720

gcctgaaacg catgttgtgg ttgttgagaa tgaagctgat aaagctggtt ttctcggcgc 9780

cgcagtgttt tatgcacaat cgctctacag acctatgttg atggtggaaa agaaattaat 9840

aactaccgcc aacactggtt tgtctgttag tcgaactatg tttgaccttt atgtagattc 9900

attgctgaac gtcctcgacg tggatcgcaa gagtctaaca agttttgtaa atgctgcgca 9960

caactctcta aaggagggtg ttcagcttga acaagttatg gataccttta ttggctgtgc 10020

ccgacgtaag tgtgctatag attctgatgt tgaaaccaag tctattacca agtccgtcat 10080

gtcggcagta aatgctggcg ttgattttac ggatgagagt tgtaataact tggtgcctac 10140

ctatgttaaa agtgacacta tcgttgcagc cgatttgggt gttcttattc agaataatgc 10200

taagcatgta caggctaatg ttgctaaagc cgctaatgtg gcttgcattt ggtctgtgga 10260

tgcttttaac cagctatctg ctgacttaca gcataggctg cgaaaagcat gttcaaaaac 10320

tggcttgaag attaagctta cttataataa gcaggaggca aatgttccta ttttaactac 10380

accgttctct cttaaagggg gcgctgtttt tagtagaatg ttacaatggt tgtttgttgc 10440

taatttgatt tgtttcattg tgttgtgggc ccttatgcca acatatgcag tgcacaaatc 10500

ggatatgcag ttgcctttat atgccagttt taaagttata gataacggtg tgctaaggga 10560

tgtgtctgtt actgacgcat gcttcgcaaa caaatttaat caattcgacc aatggtatga 10620

gtctactttt ggtcttgctt attaccgcaa ctctaaggct tgtcctgttg tggttgctgt 10680

aatagatcaa gacattggcc ataccttatt taatgttcct accacagttt taagatatgg 10740

atttcatgtg ttgcatttta taacccatgc atttgctact gatagcgtgc agtgttacac 10800

gccacatatg caaatcccct atgataattt ctatgctagt ggttgcgtgt tgtcatccct 10860

ctgtactatg cttgcgcatg cagatggaac cccgcatcct tattgttata cagggggtgt 10920

tatgcataat gcctctctgt atagttcttt ggctcctcat gtccgttata acctggctag 10980

ttcaaatggt tatatacgtt ttcccgaagt ggttagtgaa ggcattgtgc gtgttgtgcg 11040

cactcgctct atgacctact gcagggttgg tttatgtgag gaggccgagg agggtatctg 11100

ctttaatttt aatcgttcat gggtattgaa caacccgtat tatagggcca tgcctggaac 11160

tttttgtggt aggaatgctt ttgatttaat acatcaagtt ttaggaggat tagtgcggcc 11220

tattgatttc tttgccttaa cggcgagttc agtggctggt gctatccttg caattattgt 11280

cgttttggct ttctattatt taatcaagct taagcgtgcc tttggtgact acactagtgt 11340

tgtggttatc aatgtaattg tgtggtgtat aaattttctg atgctttttg tgtttcaggt 11400

ttatcccaca ttgtcttgtt tatatgcttg tttctacttc tacaccacgc tttatttccc 11460

ttcggagata agtgttgtta tgcatttgca atggcttgtc atgtatggtg ctattatgcc 11520

cttgtggttt tgcattattt acgtggcagt cgttgtttca aaccatgcat tgtggttgtt 11580

ctcttactgc cgcaaaattg gtaccgaggt tcgtagtgac ggcacatttg aggaaatggc 11640

ccttactacc tttatgatta ctaaagaatc ttattgtaag ttgaaaaact ctgtttctga 11700

tgttgctttt aacaggtact tgagtcttta caacaagtac cgttacttca gtggcaaaat 11760

ggatactgcc gcttatagag aggctgcctg ttcacaactg gcaaaggcaa tggaaacatt 11820

taaccataat aatggtaatg atgttctcta tcagcctcca accgcctctg ttactacatc 11880

atttttacag tctggtatag tgaagatggt gtcgcccacc tctaaagtgg agccttgtat 11940

tgttagtgtt acttatggta acatgacact taatgggttg tggttggatg ataaagttta 12000

ttgcccaaga catgttatct gttcttcagc tgacatgaca gaccctgatt atcctaattt 12060

gctttgtaga gtgacatcaa gtgatttttg tgttatgtct ggtcgtatga gccttactgt 12120

aatgtcttat caaatgcagg gctgccaact tgttttgact gttacactgc aaaatcctaa 12180

cacgcctaag tattccttcg gtgttgttaa gcctggtgag acatttactg tactggctgc 12240

atacaatggc agacctcaag gagccttcca tgttacgctt cgtagtagcc ataccataaa 12300

gggctccttt ctatgtggat cctgcggttc tgtaggatat gttttaactg gcgatagtgt 12360

acgatttgtt tatatgcatc agctagagtt gagtactggt tgtcataccg gtactgactt 12420

tagtgggaac ttttatggtc cctatagaga tgcgcaagtt gtacaattgc ctgttcagga 12480

ttatacgcag actgttaatg ttgtagcttg gctttatgct gctattttta acagatgcaa 12540

ctggtttgtg caaagtgata gttgttccct ggaggagttt aatgtttggg ctatgaccaa 12600

tggttttagc tcaatcaaag ccgatcttgt cttggatgcg cttgcttcta tgacaggcgt 12660

tacagttgaa caggtgttgg ccgctattaa gaggctgcat tctggattcc agggcaaaca 12720

aattttaggt agttgtgtgc ttgaagatga gctgacacca agtgatgttt atcaacaact 12780

agctggtgtc aagctacagt caaagcgcac aagagttata aaaggtacat gttgctggat 12840

attggcttca acgtttttgt tctgtagcat tatctcagca tttgtaaaat ggactatgtt 12900

tatgtatgtt actacccata tgttgggagt gacattgtgt gcactttgtt ttgtaagctt 12960

tgctatgttg ttgatcaagc ataagcattt gtatttaact atgtacatca tgcctgtgtt 13020

atgcacactg ttttacacca actatttggt tgtgtacaaa cagagtttta gaggtctagc 13080

ttatgcttgg ctttcacact ttgtccctgc tgtagattat acatatatgg atgaagtttt 13140

atatggtgtt gtgttgctag tagctatggt gtttgttacc atgcgtagca taaaccacga 13200

cgtcttttct attatgttct tggttggtag acttgtcagc ctggtatcca tgtggtattt 13260

tggagccaat ttagaggaag aggtactatt gttcctcaca tccctatttg gcacgtacac 13320

atggactact atgttgtcat tggctaccgc taaggttatt gctaaatggt tggctgtgaa 13380

tgtcttgtac ttcacagacg taccgcaaat taaattagtt ctgttgagct acttgtgtat 13440

tggttatgtg tgttgttgtt attggggaat cttgtcactc cttaatagca tttttaggat 13500

gccattgggc gtctacaatt ataaaatctc cgttcaggag ttacgttata tgaatgctaa 13560

tggcttgcgc ccacctagaa atagttttga ggccctgatg cttaatttta agctgttggg 13620

aattggtggt gtgccagtca ttgaagtatc tcaaattcaa tcaagattga cggatgttaa 13680

atgtgctaat gttgtgttgc ttaattgcct ccagcacttg catattgcat ctaattctaa 13740

gttgtggcag tattgtagta ctttgcacaa tgaaatactg gctacatctg atttgagcgt 13800

ggccttcgat aagttggctc aactcttagt tgttttattt gctaatccag cagcagtgga 13860

tagcaagtgc cttgcaagta ttgaagaagt gagcgatgat tacgttcgcg acaatactgt 13920

cttgcaagcc ttacagagtg aatttgttaa tatggctagc ttcgttgagt atgaacttgc 13980

taagaagaat ctagatgagg ctaaggctag cggctctgcc aatcaacagc agattaagca 14040

gctagagaag gcgtgtaata ttgctaagtc agcatatgag cgcgacagag ctgttgctcg 14100

taagctggaa cgtatggctg atttagctct tacaaacatg tataaagaag ctagaattaa 14160

tgataagaag agtaaggtag tgtctgcatt gcaaaccatg ctctttagta tggtgcgtaa 14220

gctagataac caagctctta attctatttt agacaacgca gttaagggtt gtgtaccttt 14280

gaatgcaata ccatcattga cttcgaacac tctgactata atagtgccag ataagcaggt 14340

ttttgatcag gttgtggata atgtgtatgt cacctatgct gggaatgtat ggcatataca 14400

gtttattcaa gatgctgatg gtgctgttaa acaattgaat gagatagatg ttaattcaac 14460

ctggcctcta gtcattgctg caaataggca taatgaagtg tctactgttg ttttgcagaa 14520

caatgagttg atgcctcaga agttgagaac tcaggttgtc aatagtggct cagatatgaa 14580

ttgtaatact cctacccagt gttactataa tactactggc acgggtaaga ttgtgtatgc 14640

tatacttagt gactgtgacg gcctgaagta cactaagata gtaaaagaag atggaaattg 14700

tgttgttttg gaattggatc ctccctgtaa gttttctgtt caggatgtga agggccttaa 14760

aattaagtac ctttactttg tgaaggggtg taatacactg gctagaggct gggttgtagg 14820

caccttatcc tcgacagtga gattgcaggc gggtacggca actgagtatg cctccaactc 14880

tgcaatactg tcgctgtgtg cgttttctgt agatcctaag aaaacgtact tggattatat 14940

aaaacagggt ggagttcccg ttactaattg tgttaagatg ttatgtgacc atgctggcac 15000

tggtatggcc attactatta agccggaggc aaccactaat caggattctt atggtggtgc 15060

ttccgtttgt atatattgcc gctcgcgtgt tgaacatcca gatgttgatg gattgtgcaa 15120

attacgcggc aagtttgtcc aagtgccctt aggcataaaa gatcctgtgt catatgtgtt 15180

gacgcatgat gtttgtcagg tttgtggctt ttggcgagat ggtagctgtt cctgtgtagg 15240

cacaggctcc cagtttcagt caaaagacac gaacttttta aacggattcg gggtacaagt 15300

gtaaatgccc gtcttgtacc ctgtgccagt ggcttggaca ctgatgttca attaagggca 15360

tttgacattt gtaatgctaa tcgagctggc attggtttgt attataaagt gaattgctgc 15420

cgcttccagc gtgtagatga ggacggcaac aagttggata agttctttgt tgttaaaaga 15480

actaatttag aagtgtataa caaggagaaa gaatgctatg agttgacaaa agaatgcggt 15540

gttgtggctg aacacgagtt cttcacattt gatgtggagg gaagtcgggt accacacata 15600

gtccgtaaag atctttcaaa gtttactatg ttagatcttt gctatgcatt gcgtcatttt 15660

gaccgcaatg attgttcaac tcttaaggaa attctcctta catatgctga gtgtgaagag 15720

tcctacttcc aaaagaagga ctggtatgat tttgttgaga atcctgatat aattaatgtg 15780

tacaagaagc ttggtcctat atttaataga gccctgctta acactgccaa gtttgcagac 15840

gcattagtgg aggcaggctt agtaggtgtt ttaacacttg ataatcaaga tttatatggt 15900

caatggtatg actttggaga ttttgtcaag acagtacctg gttgtggtgt tgccgtggca 15960

gactcttatt attcatatat gatgccaatg ctgactatgt gtcatgcgtt ggatagtgag 16020

ttgtttgtta atggtactta tagggagttt gaccttgttc agtatgattt tactgatttc 16080

aagctagagc tgttcactaa gtattttaag cattggagta tgacctacca cccgaacacc 16140

tgtgagtgcg aggatgacag gtgcattatt cattgcgcca attttaatat acttttcagc 16200

atggtcttac ctaagacctg ttttgggcct cttgttaggc agatatttgt ggatggtgtt 16260

cctttcgttg tgtcgatcgg ttaccattat aaagaattag gtgttgttat gaatatggat 16320

gtggatacac atcgttatcg cttgtctctt aaggacttgc ttttgtatgc tgcagaccct 16380

gcccttcatg tggcgtctgc tagtgcactg cttgatttgc gcacatgttg ttttagcgtt 16440

gcagctatta caagtggcgt aaaatttcaa acagttaaac ctggaaattt taatcaggat 16500

ttctacgagt ttattttgag taaaggcctg cttaaagagg ggagctccgt tgatttgaag 16560

cacttcttct ttacgcagga tggtaatgct gctattactg attacaatta ctacaagtat 16620

aatctaccca ccatggtgga tattaagcag ttgttgtttg ttttagaagt tgttaataag 16680

tacttcgaga tctatgaggg tgggtgtata cccgcaacac aggtcattgt taataattat 16740

gacaagagtg ctggctatcc atttaataaa tttggaaagg ccaggctcta ttatgaggca 16800

ttatcatttg aggagcagga tgaaatttat gcgtatacca aacgcaatgt cctgccgacc 16860

ctaactcaaa tgaatcttaa atatgctatt agtgctaaga atagggcccg caccgttgct 16920

ggtgtctcta ttctcagtac tatgactggc agaatgtttc atcaaaagtg tctaaagagt 16980

atagcagcta ctcgcggtgt tcctgtagtt ataggcacca cgaagttcta tggcggttgg 17040

gatgatatgt tacgccgcct tattaaagat gttgatagtc ctgtactcat gggttgggac 17100

tatcctaaat gtgatcgtgc tatgccaaac atactgcgta ttgttagtag tttggtgcta 17160

gcccgtaaac atgattcgtg ctgttcgcat acggatagat tctatcgtct tgcgaacgag 17220

tgcgcccaag ttttgagtga aattgttatg tgtggtggtt gttattatgt taaaccaggt 17280

ggcactagta gtggggatgc aaccactgct tttgctaatt ctgtgtttaa catttgtcaa 17340

gctgtttccg ccaatgtatg ctcgcttatg gcatgcaatg gacacaaaat tgaagatttg 17400

agtatacgcg agttacaaaa gcgcctatac tctaatgtct atcgtgcgga ccatgttgac 17460

cccgcatttg ttagtgagta ttatgagttt ttaaacaagc attttagtat gatgattttg 17520

agtgatgatg gtgttgtgtg ttataattca gagtttgcgt ccaagggtta tattgctaat 17580

ataagtgcct ttcaacaggt attatattat caaaacaacg tgtttatgtc tgaggccaaa 17640

tgttgggtag aaacagacat cgaaaaggga ccgcatgaat tttgttctca acatacaatg 17700

ctagtcaaga tggatggtga tgaagtctac cttccatacc ctgatccttc gagaatctta 17760

ggagcaggct gttttgttga tgatttactc aagactgata gcgttctctt gatagagcgt 17820

ttcgtaagtc ttgcaattga tgcttatcct ttagtatacc atgagaaccc agagtatcaa 17880

aatgtgttcc gggtatattt agaatacatc aagaagctgt acaatgatct cggtaatcag 17940

atcctggaca gctacagtgt tattttaagt acttgtgatg gtcaaaagtt tactgacgag 18000

acgttttaca agaacatgta tttaagaagt gcagtgctgc aaagcgttgg tgcctgcgtt 18060

gtctgtagtt ctcaaacatc attacgttgt ggcagttgca tacgcaagcc tttgctgtgt 18120

tgcaaatgcg cctatgatca tgttatgtcc actgatcata aatatgtcct gagtgtgtca 18180

ccatatgtgt gtaattcacc gggatgtgat gtaaatgatg ttaccaaatt gtatttaggt 18240

ggtatgtcat attattgtga ggaccataaa ccacagtatt cattcaaatt ggtgatgaat 18300

ggtatggttt ttggtttata taagcagtct tgtactggtt cgccctacat agaggatttt 18360

aataaaatcg ctagttgcaa atggacagaa gtcgatgatt atgtgctagc taatgaatgc 18420

accgaacgcc ttaaattgtt tgccgcagaa acgcagaagg ccacagaaga ggcctttaag 18480

caatgttatg cgtcagcaac gatccgtgag atcgtgagcg atcgggagtt aattttatct 18540

tgggaaattg gtaaagtccg cccgccactt aataaaaatt acgtgttcac cggctaccat 18600

tttactaata atggtaagac agttttaggt gagtatgttt ttgataagag tgagttgact 18660

aatggtgtgt attatcgcgc cacaaccact tataagttat ctgtaggtga tgtgttcatt 18720

ttaacatcac acgcagtgtc tagtttaagt gctcctacat tagtaccgca ggagaattat 18780

actagcattc gttttgctag tgtttatagt gtgcctgaga cgtttcagaa taatgtgcct 18840

aattatcagc acattggaat gaagcgctat tgtactgtac agggaccgcc tggtactggt 18900

aagtcccatc tagccattgg gctagctgtt tattattgta cagcgcgcgt ggtgtatacc 18960

gctgctagcc atgctgcagt tgacgcgctg tgtgaaaagg cacataaatt tctcaacatc 19020

aacgactgca cgcgtattgt tcctgcaaag gtgcgtgtag attgttatga taaattcaag 19080

gtcaatgaca ccactcgcaa gtatgtgttt actacaataa atgcattacc tgagttggtg 19140

actgacatta ttgtcgttga tgaagttagt atgcttacca actatgagct gtctgttatt 19200

aacagtcgtg ttagggctaa gcattatgtg tatattggcg acccggcgca gttacctgca 19260

ccacgtgtgc tactgaataa gggaactcta gaacctagat attttaattc cgttaccaag 19320

ctaatgtgtt gtttgggtcc agatattttc ttgggcacct gttatagatg ccctaaggag 19380

attgtggata cggtgtcagc cttggtttat aataataagc tgaaggctaa aaatgataat 19440

agctccatgt gctttaaggt ttattataag ggccagacta cacatgagag ttctagtgct 19500

gttaatatgc agcaaataca tttaatttcc aagtttctga aggcaaaccc cagttggagt 19560

aacgccgtat ttattagtcc ttataactcg cagaactatg ttgctaagag agtcttggga 19620

ttacaaaccc agacagtaga ctcagcgcag ggttctgaat atgattttgt tatctactca 19680

cagactgcgg aaacagcgca ttctgtcaat gtaaatagat tcaatgttgc tattacacgt 19740

gctaagaagg gtattctctg tgtcatgagt agtatgcaat tatttgagtc tcttaatttt 19800

actacactga cgttggataa gattaacaat ccacgattac agtgtactac aaatttgttt 19860

aaggattgta gcaggagcta tgtaggatat cacccagccc atgcaccatc ctttttggca 19920

gttgatgaca aatataaggt aggcggtgat ttagccgttt gccttaatgt tgctgattct 19980

gctgtcactt attcgcggct tatatcactc atgggattca agcttgactt gacccttgat 20040

ggttattgta agctgtttat aactagagat gaagctatca aacgtgttag agcctgggtt 20100

ggcttcgatg cagaaggtgc ccatgcgata cgtgatagca ttgggacaaa tttcccatta 20160

caattaggct tttcgactgg aattgatttt gttgtcgaag ccactggaat gtttgctgag 20220

agagatggtt atgtctttaa aaaggcagcc gcacgagctc ctcctggcga acaatttaaa 20280

caccttatcc cacttatgtc aagagggcag aaatgggatg tggttcgcat tagaatagta 20340

caaatgttgt cagaccacct agtggatttg gcagacagtg ttgtacttgt gacgtgggct 20400

gccagctttg agctcacatg tttgcgatat ttcgctaaag ttggaagaga agttgtgtgt 20460

agtgtctgca ccaagcgtgc gacatgtttt aattctagaa ctggatacta tggatgctgg 20520

cgacatagtt attcctgtga ttacctgtac aacccactaa tagttgacat tcaacagtgg 20580

ggatatacag gatctttaac tagcaatcat gatcctattt gcagcgtgca taagggtgct 20640

catgttgcat catctgatgc tatcatgacc cggtgtctag ctgttcatga ttgcttttgt 20700

aagtctgtta attggaattt agaatacccc attatttcaa atgaggtcag tgttaatacc 20760

tcctgcaggt tattgcagcg cgtaatgttt agggctgcga tgctatgcaa taggtatgat 20820

gtgtgttatg acattggcaa ccctaaaggt cttgcctgtg tcaaaggata tgattttaag 20880

ttctatgacg cctcccctgt tgttaagtct gttaaacagt ttgtttacaa atacgaggca 20940

cataaagatc aatttttaga tggtttgtgt atgttttgga actgcaatgt ggataagtat 21000

ccagcgaatg cagttgtgtg taggtttgac acgcgtgtgt tgaacaaatt aaatctccct 21060

ggctgtaatg gtggcagttt gtatgttaac aaacatgcat tccacaccag tccctttacc 21120

cgggctgcct tcgagaattt gaagcctatg cctttctttt attattcaga tacgccctgt 21180

gtgtatatgg aaggcatgga atctaagcag gtcgattatg tcccattgag aagcgctaca 21240

tgcatcacaa gatgcaattt aggtggcgct gtttgtttaa aacatgctga ggagtatcgt 21300

gagtaccttg agtcttacaa tacggcaacc acagcgggtt ttactttttg ggtctataag 21360

acttttgatt tttacaacct ttggaatact tttactaggc tccaaagttt agaaaatgta 21420

gtgtataacc tggtcaacgc tggacacttt gatggccggg cgggtgaact gccttgtgct 21480

gttataggtg agaaagtcat tgccaagatt caaaatgagg atgtcgtggt ctttaaaaat 21540

aacacgccat tccccactaa tgtggctgtc gaattatttg ctaagcgcag tattcggccc 21600

caccccgagc ttaagctctt tagaaatttg aatattgacg tgtgctggag tcacgtcctt 21660

tgggattatg ctaaggatag tgtgttttgc agttcgacgt ataaggtctg caaatacaca 21720

gatttacagt gcattgaaag cttgaatgta ctttttgatg gtcgtgataa tggtgctctt 21780

gaagctttta agaagtgccg gaatggcgtc tacattaaca cgacaaaaat taaaagtctg 21840

tcgatgatta aaggcccaca acgtgccgat ttgaatggcg tagttgtgga gaaagttgga 21900

gattctgatg tggaattttg gtttgctgtg cgtaaagacg gtgacgatgt tatcttcagc 21960

cgtacaggga gccttgaacc gagccattac cggagcccac aaggtaatcc gggtggtaat 22020

cgcgtgggtg atctcagcgg taatgaagct ctagcgcgtg gcactatctt tactcaaagc 22080

agattattat cttctttcac acctcgatca gagatggaga aagattttat ggatttagat 22140

gatgatgtgt tcattgcaaa atatagttta caggactacg cgtttgaaca cgttgtttat 22200

ggtagtttta accagaagat tattggaggt ttgcatttgc ttattggctt agcccgtagg 22260

cagcaaaaat ccaatctggt aattcaagag ttcgtgacat acgactctag cattcattcg 22320

tactttatca ctgacgagaa cagtggtagt agtaagagtg tgtgcactgt tattgattta 22380

ttgttagatg attttgtgga cattgtaaag tccctgaatc taaagtgtgt gagtaaggtt 22440

gttaatgtta atgtggattt taaggacttc cagtttatgt tgtggtgcaa tgaggagaag 22500

gtcatgactt tctatcctcg tttgcaggct gctgctgact ggaaacctgg ttatgttatg 22560

cctgtcttat ataagtattt ggaatcgcct ctggaaagag taaacctctg gaattatggc 22620

aagccgatta ctttacctac aggatgtatg atgaatgttg ctaagtatac tcaattatgt 22680

caatatttga gcactacaac attagcagtt ccggctaata tgcgtgtctt acaccttggt 22740

gccggttcgg ataagggtgt tgcccctggg tctgcagttc ttaggcagtg gctaccagcg 22800

ggaagtattc ttgtagataa tgatgtgaat ccatttgtga gtgacagtgt cgcctcatat 22860

tatggaaatt gtataacctt accctttgat tgtcagtggg atctgataat ttctgatatg 22920

tacgaccctc ttactaagaa cattggggag tacaacgtga gtaaagatgg attctttact 22980

tacctctgtc atttaattcg tgacaagttg gctctgggtg gcagtgttgc cataaaaata 23040

acagagtttt cttggaacgc tgagttatat agtttaatgg ggaagtttgc gttctggaca 23100

atcttttgca ccaacgtaaa cgcctcttca agtgaaggat ttttgattgg cataaattgg 23160

ttgaataaga cccgtaccga aattgacggt aaaaccatgc atgccaatta tctgttttgg 23220

agaaatagta caatgtggaa tggaggggct tacagtctct ttgacatgag taagttccct 23280

ttgaaagcgg ctggtacggc tgttgttagc cttaaaccag accaaataaa tgacttagtc 23340

ctctccttga ttgagaaggg caagttatta gtgcgtgata cacgcaaaga agtttttgtt 23400

ggcgatagcc tagtaaatgt caaataaatc tatacttgtc gtggctgtga aaatggcctt 23460

tgctgacaag cctaatcatt tcataaactt tcccctggcc caatttagtg gctttatggg 23520

taagtattta aagctacagt ctcaacttgt ggaaatgggt ttagactgta aattacagaa 23580

ggcaccacat gttagtatta ccctgcttga tattaaagca gaccaataca aacaggtgga 23640

atttgcaata caagaaataa tagatgatct ggcggcatat gagggagata ttgtctttga 23700

caaccctcac atgcttggca gatgccttgt tcttgatgtt agaggatttg aagagttgca 23760

tgaagatatt gttgaaattc tccgcagaag gggttgcacg gcagatcaat ccagacactg 23820

gattccgcac tgcactgtgg cccaatttga cgaagaaaga gaaacaaaag gaatgcaatt 23880

ctatcataaa gaacccttct acctcaagca taacaaccta ttaacggatg ctgggcttga 23940

gctcgtgaag ataggttctt ccaaaataga tgggttttat tgtagtgaac tgagtgtttg 24000

gtgtggtgag aggctttgtt ataagcctcc aacacccaaa ttcagtgata tatttggcta 24060

ttgctgcata gataaaatac gtggtgattt agaaataggc gacctgccgc aggatgatga 24120

ggaagcgtgg gccgagctaa gttaccacta tcaaagaaac acctacttct tcagacatgt 24180

gcacgataat agcatctatt ttcgtaccgt gtgtagaatg aagggttgta tgtgttgatt 24240

tgtttttaca ctattagtgt aataagctta ttattttgtt gaaaagggca ggatgtgcat 24300

agctatggct cctcgcacac tgcttttgct gatttgatgt cagctggtgt ttgggttcaa 24360

tgaacctctt aacatcgttt cacatttaaa tgatgactgg tttctatttg gtgacagtcg 24420

gtccgactgt acctatgtag aaaataacgg tcatcctaaa ttagattggc ttgacctcga 24480

cccaaagttg tgtaattcag gaaagatttc cgcaaagagt ggtaactctc tctttaggag 24540

ttttcacttc actgattttt acaattatac gggtgaggga taccaaattg tattttatga 24600

aggagttaat tttagtccca gccatggctt taaatgcctg gctcatggag ataataaaag 24660

atggatgggc aataaagctc gattttatgc ccgagtgtat gagaagatgg cccaatatag 24720

gagcctatcg tttgttaatg tgtcttatgc ctatggaggt aatgcaaagc ccgcctccat 24780

ttgcaaagac aatactttaa cactcaataa ccccaccttc atatcgaagg agtctaatta 24840

tgttgattac tactacgaga gtgaggctaa tttcacacta gaaggttgtg atgaatttat 24900

agtaccgctc tgtggtttta atggccattc caagggctcg tcgtcggatg ctgccaataa 24960

atattatact gactctcaga gttactataa tatggatatt ggtgtcttat atgggttcaa 25020

ttcgaccttg gatgttggca acactgctaa ggatccgggt cttgatctca cttgtaggta 25080

tcttgcattg actcctggta attataaggc tgtgtcctta gaatatttgt taagcttacc 25140

ctcaaaggct atttgcctcc ataagacaaa gcgctttatg cctgtgcagg tagttgactc 25200

aaggtggagt agcatccgcc agtcagacaa tatgaccgct gcagcctgtc agctgccata 25260

ttgtttcttt cgcaacacat ctgcgaatta tagtggtggc acacatgatg cgcaccatgg 25320

tgattttcat ttcaggcagt tattgtctgg tttgttatat aatgtttcct gtattgccca 25380

gcagggtgca tttctttata ataatgtgtc gtcctcttgg ccagcctatg ggtacggtca 25440

ttgtccaacg gcagctaaca ttggttatat ggcacctgtt tgtatctatg accctctccc 25500

ggtcatactg ctaggtgtgt tattgggtat agctgtgttg actattgtgt ttctgatgtt 25560

ttattttatg acggatagcg gtgttagatt gcatgaggca taatctaaac atgtttgttt 25620

ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc agaactcaat 25680

taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac aaagttttca 25740

gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc aatgttactt 25800

ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat aaccctgtcc 25860

taccatttaa tgatggtgtt tactttgctt ccactgagaa gtctaacata ataagaggct 25920

ggatttttgg tactacttta gattcgaaaa cccagtccct acttattgtt aataacgcta 25980

ctaatgttgt tatcaaagtc tgtgaatttc aattttgtaa cgatccattt ttgggtgttt 26040

attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat tctagtgcga 26100

ataattgcac ttttgaatac gtctctcagc cttttcttat ggaccttgaa ggaaaacagg 26160

gtaatttcaa aaatcttagg gaatttgtgt tcaagaatat tgatggttac ttcaagatat 26220

actctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt tcggctttag 26280

aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact ttacttgctt 26340

tacatagaag ttatttaact cctggtgatt cttcttcagg ttggacagct ggtgctgcag 26400

cttattatgt gggttatctt caacctagga cttttctact gaagtacaat gaaaatggaa 26460

ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag tgtacgttga 26520

aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc caaccaacag 26580

aatctattgt tagatttcct aacatcacaa acttgtgccc ttttggtgaa gtttttaacg 26640

ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac tgtgttgctg 26700

attattctgt cctgtataat tccgcatcat tttccacttt taagtgttat ggagtgtctc 26760

ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt gtaattagag 26820

gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat tataactaca 26880

aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat cttgattcta 26940

aggttggtgg taattataat tacctgtaca gattgtttag gaagtctaat ctcaaacctt 27000

ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt aatggtgttg 27060

aaggttttaa ttgttacttt cctctgcaat catatggttt ccaacccact aatggtgttg 27120

gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca ccagcaactg 27180

tttgtggacc taaaaagtct actaatttgg ttaagaacaa gtgtgtcaat ttcaacttca 27240

atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg cctttccaac 27300

aatttggcag agacattgct gacactactg atgctgttcg tgatccacaa acacttgaga 27360

ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca ggaacaaata 27420

cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc cctgttgcta 27480

ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct aatgtttttc 27540

aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat gagtgtgaca 27600

tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct cctcggagag 27660

caagaagtgt agctagtcaa tccatcattg cctacactat gtcacttggt gcagaaaatt 27720

cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt agcgttacca 27780

cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg tacatttgtg 27840

gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt acacaattaa 27900

accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa gtttttgcac 27960

aagtcaaaca aatttacaag acaccaccaa ttaaagattt tggcggtttt aattttagcc 28020

agatactgcc agatccatca aaaccaagca agaggtcatt tattgaagat ctactgttca 28080

acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc cttggtgata 28140

ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt ttgccacctt 28200

tgctcacaga tgaaatgatt gctcaataca cttctgcact gttagcaggt acaatcactt 28260

ctggttggac ttttggtgca ggtgctgcat tacaaatacc atttgctatg caaatggctt 28320

ataggtttaa tggtattgga gttacacaga atgttctcta tgagaaccaa aaattgattg 28380

ccaaccaatt taatagtgct attggcaaaa ttcaagactc actttcttcc acagcaagtg 28440

cacttggaaa acttcaagat gtggtcaacc aaaatgcaca agctttaaac acgcttgtta 28500

aacaacttag ctccaatttt ggtgcaattt caagtgtttt aaacgacatc ctttcacgtc 28560

ttgacaaagt tgaggctgaa gtgcaaattg ataggttgat cacaggcaga cttcaaagtt 28620

tgcagacata tgtgactcaa caattaatta gagctgcaga aatcagagct tctgctaatc 28680

ttgctgctac taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt gacttttgcg 28740

gaaagggcta tcatcttatg tcatttcctc agtcagcacc tcatggtgtc gtctttttgc 28800

atgtgactta tgtccctgca caagaaaaga acttcacaac tgctcctgcc atttgtcatg 28860

atggaaaagc acactttcct cgtgaaggtg tctttgtttc aaatggcaca cactggtttg 28920

taacacaaag gaatttttat gaaccacaaa tcattactac agacaacaca tttgtgtctg 28980

gtaactgtga tgttgtaata ggaattgtca acaacacagt ttatgatcct ttgcaacctg 29040

aattagactc attcaaggag gagcttgata aatacttcaa gaaccatacc tcaccagatg 29100

ttgatttagg tgacatctct ggcattaatg cttcagttgt aaacattcag aaagaaatcg 29160

accgcctcaa tgaggttgcc aagaatttaa atgaatctct catcgatctc caagaacttg 29220

gaaagtatga gcagtatata aaatggccat ggtacatttg gctaggtttt atagctggct 29280

tgattgccat agtaatggtg acaattatgc tttgctgtat gaccagttgc tgtagttgtc 29340

tcaagggctg ttgttcttgt ggatcctgct gcaaatttga cgaggacgac tctgagccag 29400

tgctcaaagg agtcaaatta cattacacat aactatcaca gcctctcctg gaaagacaga 29460

aaatctaaac aatttatagc attctcattg ctacctggcc ccgtaagagg cagtcatagc 29520

tatggccgtg ttggtcctaa ggctacattg gctgctgtct ttattggtcc atttattgta 29580

gcatgtatgc taggcattgg cctagtttat ttattgcaat tgcaagttca aatttttcat 29640

gttaaggata ccatacgtgt gactggcaag ccagccactg tgtcttatac tacaagtaca 29700

ccagtaacac cgagcgcgac gacgctcgat ggtactacgt atactttaat tagacccact 29760

agctcttata caagagttta tcttggtact ccaagaggtt ttgattatag tacatttggg 29820

cctaagaccc tagattatgt tactaatcta aacctcatct taattctggt cgtccatata 29880

cttttaaggc attgtccagg catatgaggc caacagccac atggatttgg catgtgagtg 29940

atgcatggtt acgccgcacg cgggactttg gtgtcattcg cctagaagat ttttgttttc 30000

aatttaatta tagccaaccc cgagttggtt attgtagagt tcctttaaag gcttggtgta 30060

gcaaccaggg taaatttgca gcgcagttta ccctaaaaag ttgcgaaaaa ccaggtcacg 30120

aaaaatttat tactagcttc acggcctacg gcagaactgt ccaacaggcc gttagcaagt 30180

tagtagaaga agctgttgat tttattcttt ttagggccac gcagctcgaa agaaatgttt 30240

aatttattcc ttacagacac agtatggtat gtggggcaga ttatttttat attcgcagtg 30300

tgtttgatgg tcaccataat tgtggttgcc ttccttgcgt ctatcaaact ttgtattcaa 30360

ctttgcggtt tatgtaatac tttggtgctg tccccttcta tttatttgta tgataggagt 30420

aagcagcttt ataagtacta taatgaagaa atgagactgc ccctattaga ggtggatgat 30480

atctaatcca aacattatga gtagtactac tcaggcccca gagcccgtct atcaatggac 30540

cgccgacgag gcagttcaat tccttaagga atggaacttc tcgttgggca ttatactact 30600

ctttattact atcatactac agttcggtta cacgagccgt agcatgttta tttatgttgt 30660

gaaaatgata atcttgtggt taatgtggcc actgactatt gttttgtgta ttttcaattg 30720

cgtgtatgcg ctaaataatg tgtatcttgg attttctata gtgtttacta tagtgtccat 30780

tgtaatctgg atcatgtatt ttgtgaacag cataaggttg tttatcagga ctggtagctg 30840

gtggagcttc aaccccgaaa caaacaacct tatgtgtata gatatgaaag gtaccgtgta 30900

tgttagaccc attattgagg attaccatac actaacagcc actattattc gtggccacct 30960

ctacatgcaa ggtgttaagc taggcaccgg tttctctttg tctgacttgc ccgcttatgt 31020

tacagttgct aaggtgtcac acctttgcac ttataagcgc gcattcttag acaaggtaga 31080

cggtgttagc ggttttgctg tttatgtgaa gtccaaggtc ggaaattacc gactgccctc 31140

aaacaaaccg agtggcgcgg acaccgcatt gttgagaacc taatctaaac tttaaggaga 31200

gaatgaatcc tatgtcggcg ctcggtggta acccctcgcg agaaagtcgg gataggacac 31260

tctctatcag aatggatgtc ttgctgtcat aacagataga gaaggttgtg gcagaccctg 31320

tatcaattag ttgaaagaga ttgcaaaata gagaatgtgt gagagaagtt agcaaggtcc 31380

tacgtctaac cataagaacg gcgataggcg ccccctggga acagctcaca tcagggtact 31440

attcctgcaa tgccctagta aatgaatgaa gttgatcatg gccaattgga agaatcacaa 31500

aaaaaaaaaa aaaaacggcc ggtttaaacg ctacagtcca agttccaagc gggatactag 31560

atgtataatg tccgccatgc agacgaaacc agtcggagat taccgagcat tctatcacgt 31620

cggcgaccaa tagtgagctt agggataaca gggtaataaa cgatccccgg gaattcactg 31680

gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 31740

gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 31800

tcccaacagt tgcgcagcct gaatggcgaa tggcgataga tccggtggat gaccttttga 31860

atgaccttta atagattata ttactaatta attggggacc ctagaggtcc ccttttttat 31920

tttaaaaatt ttttcacaaa acggtttaca agcataaagc tcggacggat cttttccgct 31980

gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca 32040

cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt 32100

cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc 32160

ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc 32220

gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac caggaagggc 32280

agcccaccta tcaaggtgtc gatgcagggg ggggggaaag ccacgttgtg tctcaaaatc 32340

tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt 32400

acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcaa 32460

ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 32520

atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 32580

tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 32640

taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 32700

atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag 32760

aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 32820

attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 32880

cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 32940

gctggcctgt tgaacaagtc tggaaagaaa tgcataagtt tttgccattc tcaccggatt 33000

cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 33060

taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 33120

tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 33180

gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 33240

aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg 33300

gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc 33360

cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta 33420

caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca 33480

ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagacgg tatcggatcg 33540

atcccccgat gtgtagcagt ggcggaccat ataggcagat cagaaggcgc ggttctccta 33600

catgagcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 33660

cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 33720

gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 33780

tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 33840

ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 33900

tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 33960

tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 34020

tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 34080

acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 34140

agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 34200

tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 34260

ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 34320

atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 34380

ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 34440

tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 34500

tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 34560

ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 34620

gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 34680

gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata 34740

atgacgaaaa aaaaaaaatt ggaaagaaaa agctgggcgc gccggccggc ccttttcatc 34800

acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata 34860

gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact 34920

ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa 34980

tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac 35040

attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat 35100

atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttttc 35160

ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata 35220

aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc 35280

gtgtaagtta caggcaagcg atcggccggc ccgggcattt aaatgcaggc cgcgtacgcg 35340

tcgacggtac cgaattcgct taaacgagct catgttcgcc ggtgaacgcg ttgaggaagc 35400

cgggcagtgc ctcggcaaaa tccttgcgtg tagacaagac atctgcgtag cagttgtcct 35460

caacaacgat gtcgaaatcc aaatcggagt gctcatcgag tcctccgtga acgtaagagc 35520

cgccgatcag aagagcgcgg aagcgaacat cggaagcgac cgcatcgcgg atgcggttca 35580

agaaagttgc atgagcttgt ggaagtgtgc tgagcataaa tgattctcct agctgttctt 35640

tgggtaagta cgccatcagg acgttgtgag tggcgcgatt tttagcggct gaaatcagcc 35700

cttgagcctg tcggcaagtc gcgtcatgag gtccatgcgc tcatgcagga tcgccacgac 35760

caacgcgggt tcgcccgcac gcggcaggca aaaaacgtag tggtgttcgc agcgggccat 35820

ccgcagcgcg ggaaagagtt cgctcatgtc cttaaacggg ccttcgccgg cggcaagcct 35880

ggctatgccc tgttccagct tagcgatata gcggcgcacc tgcgccgcgc cccactcccg 35940

gcgcgtgtag cggatgatgc cgcgtagatc ggcttcggcc tcagccgtga ggatgtaggc 36000

cgtcaagcgc gatccccgct gagttcttca tcaagaattt cgccgacgct cttggtggac 36060

accttgccgg caagcccatc gttgatgcgg ttccccagca tggttttcag ttcctgccat 36120

gcctgatcgg catcagcgtc accggggaac agacgttcga gggcgtattg cttaatggtc 36180

ttgccctgca aggcggccag ggctttcagg ctctggtgct gctggtccgt catgtcgatt 36240

gtcaggcggc tcattggata acctccataa aatacacgta accacattag cacatatgtg 36300

ggcgtgaggc tacagcgcga ggcgcattaa ggtcgggaaa atgcgctagg cgcatttaaa 36360

ttgcgtattg ctgtaatgcg ccatgccggc tagactaggc ccaaatgggt atacccaatt 36420

tgaccaaggg ggacgcgatg agggcggcca agcactaccg acaacttcta tccatcgact 36480

tcaacatcga ggcgctggcc ttcgtgcctg gacccgacgg cacacgcggc cggcgcatcc 36540

acgtcctggg gcgcgaggtc cgcgaccggc ccggcctggt cgagtacctt tcgccggcgt 36600

tcggctcgcg ggtggcgctg gacggctact gcaaggccaa tttcgatgca gtgctgcacc 36660

tggcgtaccc cgatcatcag caatggggcc acgcatgaag cgccgaagct acgccatgct 36720

gcgcgccgct gccgcgctgg ccgtcctggt cgttgcctcg ccggcatggg ccgagctgcg 36780

cggcgaggtc gtgcgcatca tcgacggcga caccatcgac gtgctggtag acaagcagcc 36840

ggtgcgcgtg cgcctggtgg acattgacgc gccggaaaag cggcaagcct tcggcgaacg 36900

tgcgcgccag gcgctggccg gcatggtgtt ccgccggcac gtcctggtcg acgagaagga 36960

caccgaccgt tacggccgca cgctgggcac cgtgtgggtc aacatggagc tggccagccg 37020

gccgccgcag ccgcgcaacg tcaacgccgc gatggttcac cagggcatgg cgtgggccta 37080

tcgcttccac ggccgcgcgg ccgaccctga aatgctgcgg ctcgaacagg aggcgcgagg 37140

caagcgcgtc ggcctctggt ccgatccgca cgccgtcgag ccgtggaaat ggcgacgcga 37200

gagcaacaac cggagggacg aaggttgaag gtcgcccgca tctacctgcg cgccagtacg 37260

gacgagcaga atcttgaacg ccaggagagc cttgtagcgg ccacgcgggc cgccgggtac 37320

tacgtcgccg gcatctaccg cgagaaggcg tccggcgcac gcgccgaccg gcccgagctg 37380

ctgcgcatga tcgcggacct gcaacctggt gaagtcgtcg ttgcggagaa gatcgaccgc 37440

atcagccgct tgccgttggc cgaggccgag cgcctggttg cgtcgatccg ggccaaaggg 37500

gccaagctgg ccgtgcctgg cgtggtggac ctgtcggagc tggccgccga ggcgaacgga 37560

gtggcgaaaa tcgttctgga atccgtccag gacatgcttt tgaagctcgc cttgcagatg 37620

gcccgcgacg actacgagga tcggcgcgag cgtcaacgtc agggtgtcca gttggcgaag 37680

gccgccggcc gctacaccgg ccgcaaacgt gacgccggca tgcacgaccg catcatcacg 37740

cttcgctccg gcggatcgag cattgccaag acggccaagc tggtcggatg cagcccgagc 37800

caggtcaaac gagtgtgggc ggcctggaac gcgcagcagc aaaaataaag ccgggcagtg 37860

cccggctttt ctcacctttt cgcgtcccgc agggccgctg cgagcgccct acctagatcc 37920

tcgctttccc cctcggtgta gtccggccag ggcacgaagg gcgcggatgc gaacctgttg 37980

agcaggtacg ccttcgggca gcggtagacc accggcgagt tcgccttttc atcccaccgg 38040

gccaggatca cgtccgcatc acagtgcatg tccttcacct ggtcgcggaa gaagccgaag 38100

gccaccatgc cgctatgttc gccgaggaac gccagttgct tcgcgctggc gatcgcgccg 38160

acgccgccgg ccaaaaccga cgccatcacc cagccgacga accagaagct ggcatgcttg 38220

cggttgacca ccgcacgcgc agccgcgacc aggacaacgg ccaagctgcc gaccagggcc 38280

atgacgaccg tgatccggcc gttgtggaaa gcgatgggct tgccagcgtc cgcttgcacg 38340

gcgtcgtaaa tgctggaccc gatgggcgcg cacatcagca cgacaggcag cagcaccagg 38400

aacatcgtcc gcgtccattg cgcgagtgcc ttgcggcgtt cgccggcggc aagcgcctcc 38460

atcatcggcg tgaagcccaa cagggccacc gcagccgcca agccggcaac gatgccgcag 38520

gcgattacat acatacatcc tccctaatgc gccttgcgca cggttgtagt cagagtccgc 38580

ggtggggcga taagctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 38640

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgggggatca 38700

ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc 38760

cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc 38820

ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcctgct 38880

gcggcgtccg ctcgtgggcc gtggcgcggg tccgcgcgcc ggcctcgtgc gcctggcgct 38940

cgcgggcgag gtccagggcg gccgtcttca cgttctgcct tgcgcagatg agatagatcg 39000

atctagcgtg gactcaaggc tctcgcgaat ggctcgcgtt ggaaactttc attgacactt 39060

gaggggcacc gcagggaaat tctcgtcctt gcgagaaccg gctatgtcgt gctgcgcatc 39120

gagcctgcgc ccttggcttg tctcgcccct ctccgcgtcg ctacggggct tccagcgcct 39180

ttccgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg 39240

caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg 39300

gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag 39360

gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga 39420

cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac 39480

agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga 39540

ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag 39600

gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg 39660

gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat 39720

ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg 39780

ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag 39840

gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt 39900

gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga 39960

agcattgacg tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag 40020

ggcggcggcc tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc 40080

atggcggggc cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg 40140

ctcgtgttcg ggggtgaatt aattccccgg atcgatccgt cagcttcacg ctgccgcaag 40200

cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa 40260

cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc 40320

gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt 40380

ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag 40440

ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca 40500

agatcgacgg atcgatccgg ggaattaatt ccggggcaat cccgcaagga gggtga 40556

<210> 40

<211> 38383

<212> DNA

<213> Artificial sequence

<220>

<223> pMR10Y_COVAX191_delHEN

<400> 40

atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc 60

gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc 120

cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa 180

ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtctcgaa 240

caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc 300

aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc 360

gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt 420

gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc 480

accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc 540

aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg 600

gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc 660

acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag 720

gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt 780

gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa 840

acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac 900

tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc 960

gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg 1020

tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa 1080

gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc 1140

aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagccac tcgatcgagg 1200

tcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 1260

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 1320

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 1380

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttccat 1440

gggatatcga gatctcctgc agagctctag agtcgagact agtctcgacg ggcccggtac 1500

cccctcgagg gggccgcact taagttacgc gtggatcgtg gagctttcgg gttttaacta 1560

taacggtcct aaggtagcga actcgggtct tgccttaatc ccaacaaccg gattatctac 1620

acggatttca atagctgata tagcgaatca ccgagattaa ttaataatac gactcactat 1680

agtataagag tgattggcgt ccgtacgtac cctctcaact ctaaaactct tgtagtttaa 1740

atctaatcta aactttataa acggcacttc ctgcgtgtcc atgcccgcgg gcctggtctt 1800

gtcatagtgc tgacatttgt agttccttga ctttcgttct ctgccagtga cgtgtccatt 1860

cggcgccagc agcccaccca taggttgcat aatggcaaag atgggcaaat acggcctggg 1920

cttcaaatgg gccccagaat ttccatggat gcttccgaac gcatcggaga agttgggtaa 1980

ccctgagagg tcagaggagg atgggttttg cccctctgct gcgcaagaac cgaaagttaa 2040

aggaaaaact ttggttaatc acgtgagggt gaattgtagc cggcttccag ctttggaatg 2100

ctgtgttcag tctgccataa tccgtgatat ttttgtagat gaggatcccc agaaggtgga 2160

ggcctcaact atgatggcat tgcagttcgg tagtgccgtc ttggttaagc catccaagcg 2220

cttgtctatt caggcatgga ctaatttggg tgtgcttccc aaaacagctg ccatggggtt 2280

gttcaagcgc gtctgcctgt gtaacaccag ggagtgctct tgtgacgccc acgtggcctt 2340

tcaccttttt acggtccaac ccgatggtgt atgcctgggt aatggccgtt ttataggctg 2400

gttcgttcca gtcacagcca taccggagta tgcgaagcag tggttgcaac cctggtccat 2460

ccttcttcgt aagggtggta acaaagggtc tgtgacatcc ggccacttcc gccgcgctgt 2520

taccatgcct gtgtatgact ttaatgtaga ggatgcttgt gaggaggttc atcttaaccc 2580

gaagggtaag tactcctgca aggcgtatgc cctgctgaag ggctatcgcg gtgttaagcc 2640

catcctgttt gtggaccagt atggttgcga ctatactgga tgtctcgcca agggtcttga 2700

ggactatggc gatctcacct tgagtgagat gaaggagttg ttccctgtgt ggcgtgactc 2760

cttggatagt gaagtccttg tggcttggca cgttgatcga gatcctcggg ctgctatgcg 2820

tctgcagact cttgctactg tacgttgcat tgattatgtg ggccaaccga ccgaggatgt 2880

ggtggatgga gatgtggtag tgcgtgagcc tgctcatctt ctcgcagcca atgccattgt 2940

taaaagactc ccccgtttgg tggagactat gctgtatacg gattcgtccg ttacagaatt 3000

ctgttataaa accaagctgt gtgaatgcgg ttttatcacg cagtttggct atgtggattg 3060

ttgtggtgac acctgtgatt ttcgtgggtg ggttgccggc aatatgatgg atggctttcc 3120

atgtccaggg tgtaccaaaa attatatgcc ctgggaattg gaggcccagt catcaggtgt 3180

tataccagaa ggaggtgttc tattcactca gagcactgat acagtgaatc gtgagtcctt 3240

taagctctac ggtcatgctg ttgtgccttt tggttctgct gtgtattgga gcccttgccc 3300

aggtatgtgg cttccagtaa tttggtcgtc ggttaagtca tactctggtt tgacttatac 3360

aggagtagtt ggttgtaagg caattgttca agagacagac gctatatgtc gttctctgta 3420

tatggattat gtccagcaca agtgtggcaa tctcgagcag agagctatcc ttggattgga 3480

cgatgtctat catagacagt tgcttgtgaa taggggtgac tatagtctcc tccttgagaa 3540

tgtggatttg tttgttaagc ggcgcgctga atttgcttgc aaattcgcca cctgtggaga 3600

tggtcttgta cccctcctac tagatggttt agtgccccgc agttattatt tgattaagag 3660

tggtcaagct ttcacctcta tgatggttaa ttttagccat gaggtgactg acatgtgtat 3720

ggacatggct ttattgttca tgcatgatgt taaagtggcc actaagtatg ttaagaaggt 3780

tactggcaaa ctggccgtgc gctttaaagc gttgggtgta gccgttgtca gaaaaattac 3840

tgaatggttt gatttagccg tggacattgc tgctagtgcc gctggatggc tttgctacca 3900

gctggtaaat ggcttatttg cagtggccaa tggtgttata acctttgtac aggaggtgcc 3960

tgagcttgtc aagaattttg ttgacaagtt caaggcattt ttcaaggttt tgatcgactc 4020

tatgtcggtt tctatcttgt ctggacttac tgttgtcaag actgcctcaa atagggtgtg 4080

tcttgctggc agtaaggttt atgaagttgt gcagaaatct ttgtctgcat atgttatgcc 4140

tgtgggttgc agcgaagcca cttgtttggt gggtgagatt gaacctgcag tttttgaaga 4200

tgatgttgtt gatgtggtta aagccccatt aacatatcaa ggctgttgta agccacccac 4260

ttctttcgag aagatttgta ttgtggataa attgtatatg gccaagtgtg gtgatcaatt 4320

ttaccctgtg gttgttgata acgacactgt tggcgtgtta gatcagtgct ggaggtttcc 4380

ctgtgcgggc aagaaagtcg agtttaacga caagcccaaa gtcaggaaga taccctccac 4440

ccgtaagatt aagatcacct tcgcactgga tgcgaccttt gatagtgttc tttcgaaggc 4500

gtgttcagag tttgaagttg ataaagatgt tacattggat gagctgcttg atgttgtgct 4560

tgacgcagtt gagagtacgc tcagcccttg taaggagcat gatgtgatag gcacaaaagt 4620

ttgtgcttta cttgataggt tggcaggaga ttatgtctat ctttttgatg agggaggcga 4680

tgaagtgatc gccccgagga tgtattgttc cttttctgct cctgatgacg aggactgcgt 4740

tgcagcggat gttgtagatg cagatgaaaa ccaagatgat gatgccgagg actcagcagt 4800

ccttgtcgct gatacccaag aagaggacgg cgttgccaag gggcaggttg aggcggattc 4860

ggaaatttgc gttgcgcata ctggtagtca agaagaattg gctgagcctg atgctgtcgg 4920

atctcaaact cccatcgcct ctgctgagga aaccgaagtc ggagaggcaa gcgacaggga 4980

agggattgct gaggcgaagg caactgtgtg tgctgatgct gtagatgcct gccccgatca 5040

agtggaggca tttgaaattg aaaaggtcga ggactctatc ttggatgagc ttcaaactga 5100

acttaatgcg ccagcggaca agacctatga ggatgtcttg gcattcgatg ccgtatgctc 5160

agaggcgttg tctgcattct atgctgtgcc gagtgatgag acgcacttta aagtgtgtgg 5220

attctattcg cctgctatag agcgcactaa ttgttggctg cgttctactt tgatagtaat 5280

gcagagtcta cctttggaat ttaaagactt ggagatgcaa aagctctggt tgtcttacaa 5340

ggccggctat gaccaatgct ttgtggacaa actagttaag agcgtgccca agtctattat 5400

ccttccacaa ggtggttatg tggcagattt tgcctatttc tttctaagcc agtgtagctt 5460

taaagcttat gctaactggc gttgtttaga gtgtgacatg gagttaaagc ttcaaggctt 5520

ggacgccatg tttttctatg gggacgttgt gtctcatatg tgcaagtgtg gtaatagcat 5580

gaccttgttg tctgcagata taccctacac tttgcatttt ggagtgcgag atgataagtt 5640

ttgcgctttt tacacgccaa gaaaggtctt tagggctgct tgtgcggtag atgttaatga 5700

ttgtcactct atggctgtag tagagggcaa gcaaattgat ggtaaagtgg ttaccaaatt 5760

tattggtgac aaatttgatt ttatggtggg ttacgggatg acatttagta tgtctccttt 5820

tgaactcgcc cagttatatg gttcatgtat aacaccaaat gtttgttttg ttaaaggaga 5880

tgttataaag gttgttcgct tagttaatgc tgaagtcatt gttaaccctg ctaatgggcg 5940

tatggctcat ggtgccggcg tcgccggcgc catagctgaa aaggcgggca gtgcttttat 6000

taaagaaacc tccgatatgg tgaaggctca gggcgtttgc caggttggtg aatgctatga 6060

atctgccggt ggtaagttat gtaaaaaggt gcttaacatt gtagggccag atgcgcgagg 6120

gcatggcaag caatgctatt cacttttaga gcgtgcttat cagcatatta ataagtgtga 6180

caatgttgtc actactttaa tttcggctgg tatatttagt gtgcctactg atgtctccct 6240

aacttactta cttggtgtag tgacaaagaa tgtcattctt gtcagtaaca accaggatga 6300

ttttgatgtg atagagaagt gtcaggtgac ctccgttgct ggtaccaaag cgctatcact 6360

tcaattggcc aaaaatttgt gccgtgatgt aaagtttgtg acgaatgcat gtagttcgct 6420

ttttagtgaa tcttgctttg tctcaagcta tgatgtgttg caggaagttg aagcgctgcg 6480

acatgatata caattggatg atgatgctcg tgtctttgtg caggctaata tggactgtct 6540

gcccacagac tggcgtctcg ttaacaaatt tgatagtgtt gatggtgtta gaaccattaa 6600

gtattttgaa tgcccgggcg ggatttttgt atccagccag ggcaaaaagt ttggttatgt 6660

tcagaatggt tcatttaagg aggcgagtgt tagccaaata agggctttac tcgctaataa 6720

ggttgatgtc ttgtgtactg ttgatggtgt taacttccgc tcctgctgcg tagcagaggg 6780

tgaagttttt ggcaagacat taggttcagt cttttgtgat ggcataaatg tcaccaaagt 6840

taggtgtagt gccatttaca agggtaaggt tttctttcag tacagtgatt tgtccgaggc 6900

agatcttgtg gctgttaaag atgcctttgg ttttgatgaa ccacaactgc tgaagtacta 6960

cactatgctt ggcatgtgta agtggccagt agttgtttgt ggcaattatt ttgctttcaa 7020

gcagtcaaat aataattgct acatcaacgt ggcatgttta atgctgcaac acttgagttt 7080

aaagtttcct aagtggcaat ggcaagaggc ttggaacgag ttccgctctg gtaaaccact 7140

aaggtttgtg tccttggtat tagcaaaggg cagctttaaa tttaatgaac cttctgattc 7200

tatcgatttt atgcgtgtgg tgctacgtga agcagatttg agtggtgcca cgtgcaattt 7260

ggaatttgtt tgtaaatgtg gtgtgaagca agagcagcgc aaaggtgttg acgctgttat 7320

gcattttggt acgttggata aaggtgatct tgtcaggggt tataatatcg catgtacgtg 7380

cggtagtaaa cttgtgcatt gcacccaatt taacgtacca tttttaattt gctccaacac 7440

accagagggt aggaaactgc ccgacgatgt tgttgcagct aatattttta ctggtggtag 7500

tgtgggccat tacacgcatg tgaaatgtaa acccaagtac cagctttatg atgcttgtaa 7560

tgttaataag gtttcggagg ctaagggtaa ttttaccgat tgcctctacc ttaaaaattt 7620

aaagcaaacc ttctcgtctg tgctgacgac tttttattta gatgacgtaa agtgtgtgga 7680

gtataagcca gatttatcgc agtattactg tgagtctggt aaatattata caaaacccat 7740

tattaaggcc caatttagaa catttgagaa ggttgatggt gtctatacca actttaaatt 7800

ggtgggacat agtattgctg aaaaactcaa tgctaagctg ggatttgatt gtaattctcc 7860

ctttgtggag tacaaaatta cagagtggcc aacagctact ggagatgtgg tgttggctag 7920

tgatgatttg tatgtaagtc ggtacttaag cgggtgcatt acttttggta aaccggttgt 7980

ctggcttggc catgaggaag catcgctgaa atctctcaca tattttaata gacctagtgt 8040

cgtttgtgaa aataaattta acgtgttgcc cgttgatgtc agtgaaccca cggacaaggg 8100

gcctgtgcct gctgcagtcc ttgttaccgg cgtccctgga gctgatgcgt cagctggtgc 8160

cggtattgcc aaggagcaaa aagcctgtgc ttctgctagt gtggaggatc aggttgttac 8220

ggaggttcgt caagagccat ctgtttcagc tgctgatgtc aaagaggtta aattgaatgg 8280

tgttaaaaag cctgttaagg tggaaggtag tgtggttgtt aatgatccca ctagcgaaac 8340

caaagttgtt aaaagtttgt ctattgttga tgtctatgat atgttcctga cagggtgtaa 8400

gtatgtggtt tggactgcta atgagttgtc tcgactagta aattcaccga ctgttaggga 8460

gtatgtgaag tggggtatgg gaaagattgt aacacccgct aagttgttgt tgttaagaga 8520

tgagaagcaa gagttcgtag cgccaaaagt agtcaaggcg aaagctattg cctgctattg 8580

tgctgtgaag tggtttctcc tctattgttt tagttggata aagtttaata ctgacaataa 8640

ggttatatac accacagaag tagcttcaaa gcttactttc aagttgtgct gtttggcctt 8700

taagaatgcc ttacagacgt ttaattggag cgttgtgtct aggggctttt tcctagttgc 8760

aacggtcttt ttactctggt ttaacttttt gtatgctaat gttattttga gtgacttcta 8820

tttgcctaat attgggcctc tccctacgtt tgtgggacag atagttgcgt ggtttaagac 8880

tacatttggt gtgtcaacca tctgtgattt ctaccaggtg acggatttgg gctatagaag 8940

ttcgttttgt aatggaagta tggtatgtga actatgcttc tcaggttttg atatgctgga 9000

caactatgat gctataaatg ttgttcaaca cgttgtagat aggcgtttgt cctttgacta 9060

tattagccta tttaaactgg tagttgagct tgtaatcggc tactctcttt atactgtgtg 9120

cttctaccca ctgtttgtcc ttattggaat gcagttattg accacatggt tgcctgaatt 9180

ctttatgctg gagactatgc attggagtgc tcgtttgttt gtgtttgttg ccaatatgct 9240

tccagctttt acgttactgc gattttacat cgtggtgaca gctatgtata aggtctattg 9300

tctttgtaga catgttatgt atggatgtag taagcctggt tgcttgtttt gttataagag 9360

aaaccgtagt gtccgtgtta agtgtagcac cgttgttggt ggttcactac gctattacga 9420

tgtaatggct aacggcggca caggtttctg tacaaagcac cagtggaact gtcttaattg 9480

caattcctgg aaaccaggca atacattcat aactcatgaa gcagcggcgg acctctctaa 9540

ggagttgaaa cgccctgtga atccaacaga ttctgcttat tactcggtca cagaggttaa 9600

gcaggttggt tgttccatgc gtttgttcta cgagagagat ggacagcgtg tttatgatga 9660

tgttaatgct agtttgtttg tggacatgaa tggtctgctg cattctaaag ttaaaggtgt 9720

gcctgaaacg catgttgtgg ttgttgagaa tgaagctgat aaagctggtt ttctcggcgc 9780

cgcagtgttt tatgcacaat cgctctacag acctatgttg atggtggaaa agaaattaat 9840

aactaccgcc aacactggtt tgtctgttag tcgaactatg tttgaccttt atgtagattc 9900

attgctgaac gtcctcgacg tggatcgcaa gagtctaaca agttttgtaa atgctgcgca 9960

caactctcta aaggagggtg ttcagcttga acaagttatg gataccttta ttggctgtgc 10020

ccgacgtaag tgtgctatag attctgatgt tgaaaccaag tctattacca agtccgtcat 10080

gtcggcagta aatgctggcg ttgattttac ggatgagagt tgtaataact tggtgcctac 10140

ctatgttaaa agtgacacta tcgttgcagc cgatttgggt gttcttattc agaataatgc 10200

taagcatgta caggctaatg ttgctaaagc cgctaatgtg gcttgcattt ggtctgtgga 10260

tgcttttaac cagctatctg ctgacttaca gcataggctg cgaaaagcat gttcaaaaac 10320

tggcttgaag attaagctta cttataataa gcaggaggca aatgttccta ttttaactac 10380

accgttctct cttaaagggg gcgctgtttt tagtagaatg ttacaatggt tgtttgttgc 10440

taatttgatt tgtttcattg tgttgtgggc ccttatgcca acatatgcag tgcacaaatc 10500

ggatatgcag ttgcctttat atgccagttt taaagttata gataacggtg tgctaaggga 10560

tgtgtctgtt actgacgcat gcttcgcaaa caaatttaat caattcgacc aatggtatga 10620

gtctactttt ggtcttgctt attaccgcaa ctctaaggct tgtcctgttg tggttgctgt 10680

aatagatcaa gacattggcc ataccttatt taatgttcct accacagttt taagatatgg 10740

atttcatgtg ttgcatttta taacccatgc atttgctact gatagcgtgc agtgttacac 10800

gccacatatg caaatcccct atgataattt ctatgctagt ggttgcgtgt tgtcatccct 10860

ctgtactatg cttgcgcatg cagatggaac cccgcatcct tattgttata cagggggtgt 10920

tatgcataat gcctctctgt atagttcttt ggctcctcat gtccgttata acctggctag 10980

ttcaaatggt tatatacgtt ttcccgaagt ggttagtgaa ggcattgtgc gtgttgtgcg 11040

cactcgctct atgacctact gcagggttgg tttatgtgag gaggccgagg agggtatctg 11100

ctttaatttt aatcgttcat gggtattgaa caacccgtat tatagggcca tgcctggaac 11160

tttttgtggt aggaatgctt ttgatttaat acatcaagtt ttaggaggat tagtgcggcc 11220

tattgatttc tttgccttaa cggcgagttc agtggctggt gctatccttg caattattgt 11280

cgttttggct ttctattatt taatcaagct taagcgtgcc tttggtgact acactagtgt 11340

tgtggttatc aatgtaattg tgtggtgtat aaattttctg atgctttttg tgtttcaggt 11400

ttatcccaca ttgtcttgtt tatatgcttg tttctacttc tacaccacgc tttatttccc 11460

ttcggagata agtgttgtta tgcatttgca atggcttgtc atgtatggtg ctattatgcc 11520

cttgtggttt tgcattattt acgtggcagt cgttgtttca aaccatgcat tgtggttgtt 11580

ctcttactgc cgcaaaattg gtaccgaggt tcgtagtgac ggcacatttg aggaaatggc 11640

ccttactacc tttatgatta ctaaagaatc ttattgtaag ttgaaaaact ctgtttctga 11700

tgttgctttt aacaggtact tgagtcttta caacaagtac cgttacttca gtggcaaaat 11760

ggatactgcc gcttatagag aggctgcctg ttcacaactg gcaaaggcaa tggaaacatt 11820

taaccataat aatggtaatg atgttctcta tcagcctcca accgcctctg ttactacatc 11880

atttttacag tctggtatag tgaagatggt gtcgcccacc tctaaagtgg agccttgtat 11940

tgttagtgtt acttatggta acatgacact taatgggttg tggttggatg ataaagttta 12000

ttgcccaaga catgttatct gttcttcagc tgacatgaca gaccctgatt atcctaattt 12060

gctttgtaga gtgacatcaa gtgatttttg tgttatgtct ggtcgtatga gccttactgt 12120

aatgtcttat caaatgcagg gctgccaact tgttttgact gttacactgc aaaatcctaa 12180

cacgcctaag tattccttcg gtgttgttaa gcctggtgag acatttactg tactggctgc 12240

atacaatggc agacctcaag gagccttcca tgttacgctt cgtagtagcc ataccataaa 12300

gggctccttt ctatgtggat cctgcggttc tgtaggatat gttttaactg gcgatagtgt 12360

acgatttgtt tatatgcatc agctagagtt gagtactggt tgtcataccg gtactgactt 12420

tagtgggaac ttttatggtc cctatagaga tgcgcaagtt gtacaattgc ctgttcagga 12480

ttatacgcag actgttaatg ttgtagcttg gctttatgct gctattttta acagatgcaa 12540

ctggtttgtg caaagtgata gttgttccct ggaggagttt aatgtttggg ctatgaccaa 12600

tggttttagc tcaatcaaag ccgatcttgt cttggatgcg cttgcttcta tgacaggcgt 12660

tacagttgaa caggtgttgg ccgctattaa gaggctgcat tctggattcc agggcaaaca 12720

aattttaggt agttgtgtgc ttgaagatga gctgacacca agtgatgttt atcaacaact 12780

agctggtgtc aagctacagt caaagcgcac aagagttata aaaggtacat gttgctggat 12840

attggcttca acgtttttgt tctgtagcat tatctcagca tttgtaaaat ggactatgtt 12900

tatgtatgtt actacccata tgttgggagt gacattgtgt gcactttgtt ttgtaagctt 12960

tgctatgttg ttgatcaagc ataagcattt gtatttaact atgtacatca tgcctgtgtt 13020

atgcacactg ttttacacca actatttggt tgtgtacaaa cagagtttta gaggtctagc 13080

ttatgcttgg ctttcacact ttgtccctgc tgtagattat acatatatgg atgaagtttt 13140

atatggtgtt gtgttgctag tagctatggt gtttgttacc atgcgtagca taaaccacga 13200

cgtcttttct attatgttct tggttggtag acttgtcagc ctggtatcca tgtggtattt 13260

tggagccaat ttagaggaag aggtactatt gttcctcaca tccctatttg gcacgtacac 13320

atggactact atgttgtcat tggctaccgc taaggttatt gctaaatggt tggctgtgaa 13380

tgtcttgtac ttcacagacg taccgcaaat taaattagtt ctgttgagct acttgtgtat 13440

tggttatgtg tgttgttgtt attggggaat cttgtcactc cttaatagca tttttaggat 13500

gccattgggc gtctacaatt ataaaatctc cgttcaggag ttacgttata tgaatgctaa 13560

tggcttgcgc ccacctagaa atagttttga ggccctgatg cttaatttta agctgttggg 13620

aattggtggt gtgccagtca ttgaagtatc tcaaattcaa tcaagattga cggatgttaa 13680

atgtgctaat gttgtgttgc ttaattgcct ccagcacttg catattgcat ctaattctaa 13740

gttgtggcag tattgtagta ctttgcacaa tgaaatactg gctacatctg atttgagcgt 13800

ggccttcgat aagttggctc aactcttagt tgttttattt gctaatccag cagcagtgga 13860

tagcaagtgc cttgcaagta ttgaagaagt gagcgatgat tacgttcgcg acaatactgt 13920

cttgcaagcc ttacagagtg aatttgttaa tatggctagc ttcgttgagt atgaacttgc 13980

taagaagaat ctagatgagg ctaaggctag cggctctgcc aatcaacagc agattaagca 14040

gctagagaag gcgtgtaata ttgctaagtc agcatatgag cgcgacagag ctgttgctcg 14100

taagctggaa cgtatggctg atttagctct tacaaacatg tataaagaag ctagaattaa 14160

tgataagaag agtaaggtag tgtctgcatt gcaaaccatg ctctttagta tggtgcgtaa 14220

gctagataac caagctctta attctatttt agacaacgca gttaagggtt gtgtaccttt 14280

gaatgcaata ccatcattga cttcgaacac tctgactata atagtgccag ataagcaggt 14340

ttttgatcag gttgtggata atgtgtatgt cacctatgct gggaatgtat ggcatataca 14400

gtttattcaa gatgctgatg gtgctgttaa acaattgaat gagatagatg ttaattcaac 14460

ctggcctcta gtcattgctg caaataggca taatgaagtg tctactgttg ttttgcagaa 14520

caatgagttg atgcctcaga agttgagaac tcaggttgtc aatagtggct cagatatgaa 14580

ttgtaatact cctacccagt gttactataa tactactggc acgggtaaga ttgtgtatgc 14640

tatacttagt gactgtgacg gcctgaagta cactaagata gtaaaagaag atggaaattg 14700

tgttgttttg gaattggatc ctccctgtaa gttttctgtt caggatgtga agggccttaa 14760

aattaagtac ctttactttg tgaaggggtg taatacactg gctagaggct gggttgtagg 14820

caccttatcc tcgacagtga gattgcaggc gggtacggca actgagtatg cctccaactc 14880

tgcaatactg tcgctgtgtg cgttttctgt agatcctaag aaaacgtact tggattatat 14940

aaaacagggt ggagttcccg ttactaattg tgttaagatg ttatgtgacc atgctggcac 15000

tggtatggcc attactatta agccggaggc aaccactaat caggattctt atggtggtgc 15060

ttccgtttgt atatattgcc gctcgcgtgt tgaacatcca gatgttgatg gattgtgcaa 15120

attacgcggc aagtttgtcc aagtgccctt aggcataaaa gatcctgtgt catatgtgtt 15180

gacgcatgat gtttgtcagg tttgtggctt ttggcgagat ggtagctgtt cctgtgtagg 15240

cacaggctcc cagtttcagt caaaagacac gaacttttta aacggattcg gggtacaagt 15300

gtaaatgccc gtcttgtacc ctgtgccagt ggcttggaca ctgatgttca attaagggca 15360

tttgacattt gtaatgctaa tcgagctggc attggtttgt attataaagt gaattgctgc 15420

cgcttccagc gtgtagatga ggacggcaac aagttggata agttctttgt tgttaaaaga 15480

actaatttag aagtgtataa caaggagaaa gaatgctatg agttgacaaa agaatgcggt 15540

gttgtggctg aacacgagtt cttcacattt gatgtggagg gaagtcgggt accacacata 15600

gtccgtaaag atctttcaaa gtttactatg ttagatcttt gctatgcatt gcgtcatttt 15660

gaccgcaatg attgttcaac tcttaaggaa attctcctta catatgctga gtgtgaagag 15720

tcctacttcc aaaagaagga ctggtatgat tttgttgaga atcctgatat aattaatgtg 15780

tacaagaagc ttggtcctat atttaataga gccctgctta acactgccaa gtttgcagac 15840

gcattagtgg aggcaggctt agtaggtgtt ttaacacttg ataatcaaga tttatatggt 15900

caatggtatg actttggaga ttttgtcaag acagtacctg gttgtggtgt tgccgtggca 15960

gactcttatt attcatatat gatgccaatg ctgactatgt gtcatgcgtt ggatagtgag 16020

ttgtttgtta atggtactta tagggagttt gaccttgttc agtatgattt tactgatttc 16080

aagctagagc tgttcactaa gtattttaag cattggagta tgacctacca cccgaacacc 16140

tgtgagtgcg aggatgacag gtgcattatt cattgcgcca attttaatat acttttcagc 16200

atggtcttac ctaagacctg ttttgggcct cttgttaggc agatatttgt ggatggtgtt 16260

cctttcgttg tgtcgatcgg ttaccattat aaagaattag gtgttgttat gaatatggat 16320

gtggatacac atcgttatcg cttgtctctt aaggacttgc ttttgtatgc tgcagaccct 16380

gcccttcatg tggcgtctgc tagtgcactg cttgatttgc gcacatgttg ttttagcgtt 16440

gcagctatta caagtggcgt aaaatttcaa acagttaaac ctggaaattt taatcaggat 16500

ttctacgagt ttattttgag taaaggcctg cttaaagagg ggagctccgt tgatttgaag 16560

cacttcttct ttacgcagga tggtaatgct gctattactg attacaatta ctacaagtat 16620

aatctaccca ccatggtgga tattaagcag ttgttgtttg ttttagaagt tgttaataag 16680

tacttcgaga tctatgaggg tgggtgtata cccgcaacac aggtcattgt taataattat 16740

gacaagagtg ctggctatcc atttaataaa tttggaaagg ccaggctcta ttatgaggca 16800

ttatcatttg aggagcagga tgaaatttat gcgtatacca aacgcaatgt cctgccgacc 16860

ctaactcaaa tgaatcttaa atatgctatt agtgctaaga atagggcccg caccgttgct 16920

ggtgtctcta ttctcagtac tatgactggc agaatgtttc atcaaaagtg tctaaagagt 16980

atagcagcta ctcgcggtgt tcctgtagtt ataggcacca cgaagttcta tggcggttgg 17040

gatgatatgt tacgccgcct tattaaagat gttgatagtc ctgtactcat gggttgggac 17100

tatcctaaat gtgatcgtgc tatgccaaac atactgcgta ttgttagtag tttggtgcta 17160

gcccgtaaac atgattcgtg ctgttcgcat acggatagat tctatcgtct tgcgaacgag 17220

tgcgcccaag ttttgagtga aattgttatg tgtggtggtt gttattatgt taaaccaggt 17280

ggcactagta gtggggatgc aaccactgct tttgctaatt ctgtgtttaa catttgtcaa 17340

gctgtttccg ccaatgtatg ctcgcttatg gcatgcaatg gacacaaaat tgaagatttg 17400

agtatacgcg agttacaaaa gcgcctatac tctaatgtct atcgtgcgga ccatgttgac 17460

cccgcatttg ttagtgagta ttatgagttt ttaaacaagc attttagtat gatgattttg 17520

agtgatgatg gtgttgtgtg ttataattca gagtttgcgt ccaagggtta tattgctaat 17580

ataagtgcct ttcaacaggt attatattat caaaacaacg tgtttatgtc tgaggccaaa 17640

tgttgggtag aaacagacat cgaaaaggga ccgcatgaat tttgttctca acatacaatg 17700

ctagtcaaga tggatggtga tgaagtctac cttccatacc ctgatccttc gagaatctta 17760

ggagcaggct gttttgttga tgatttactc aagactgata gcgttctctt gatagagcgt 17820

ttcgtaagtc ttgcaattga tgcttatcct ttagtatacc atgagaaccc agagtatcaa 17880

aatgtgttcc gggtatattt agaatacatc aagaagctgt acaatgatct cggtaatcag 17940

atcctggaca gctacagtgt tattttaagt acttgtgatg gtcaaaagtt tactgacgag 18000

acgttttaca agaacatgta tttaagaagt gcagtgctgc aaagcgttgg tgcctgcgtt 18060

gtctgtagtt ctcaaacatc attacgttgt ggcagttgca tacgcaagcc tttgctgtgt 18120

tgcaaatgcg cctatgatca tgttatgtcc actgatcata aatatgtcct gagtgtgtca 18180

ccatatgtgt gtaattcacc gggatgtgat gtaaatgatg ttaccaaatt gtatttaggt 18240

ggtatgtcat attattgtga ggaccataaa ccacagtatt cattcaaatt ggtgatgaat 18300

ggtatggttt ttggtttata taagcagtct tgtactggtt cgccctacat agaggatttt 18360

aataaaatcg ctagttgcaa atggacagaa gtcgatgatt atgtgctagc taatgaatgc 18420

accgaacgcc ttaaattgtt tgccgcagaa acgcagaagg ccacagaaga ggcctttaag 18480

caatgttatg cgtcagcaac gatccgtgag atcgtgagcg atcgggagtt aattttatct 18540

tgggaaattg gtaaagtccg cccgccactt aataaaaatt acgtgttcac cggctaccat 18600

tttactaata atggtaagac agttttaggt gagtatgttt ttgataagag tgagttgact 18660

aatggtgtgt attatcgcgc cacaaccact tataagttat ctgtaggtga tgtgttcatt 18720

ttaacatcac acgcagtgtc tagtttaagt gctcctacat tagtaccgca ggagaattat 18780

actagcattc gttttgctag tgtttatagt gtgcctgaga cgtttcagaa taatgtgcct 18840

aattatcagc acattggaat gaagcgctat tgtactgtac agggaccgcc tggtactggt 18900

aagtcccatc tagccattgg gctagctgtt tattattgta cagcgcgcgt ggtgtatacc 18960

gctgctagcc atgctgcagt tgacgcgctg tgtgaaaagg cacataaatt tctcaacatc 19020

aacgactgca cgcgtattgt tcctgcaaag gtgcgtgtag attgttatga taaattcaag 19080

gtcaatgaca ccactcgcaa gtatgtgttt actacaataa atgcattacc tgagttggtg 19140

actgacatta ttgtcgttga tgaagttagt atgcttacca actatgagct gtctgttatt 19200

aacagtcgtg ttagggctaa gcattatgtg tatattggcg acccggcgca gttacctgca 19260

ccacgtgtgc tactgaataa gggaactcta gaacctagat attttaattc cgttaccaag 19320

ctaatgtgtt gtttgggtcc agatattttc ttgggcacct gttatagatg ccctaaggag 19380

attgtggata cggtgtcagc cttggtttat aataataagc tgaaggctaa aaatgataat 19440

agctccatgt gctttaaggt ttattataag ggccagacta cacatgagag ttctagtgct 19500

gttaatatgc agcaaataca tttaatttcc aagtttctga aggcaaaccc cagttggagt 19560

aacgccgtat ttattagtcc ttataactcg cagaactatg ttgctaagag agtcttggga 19620

ttacaaaccc agacagtaga ctcagcgcag ggttctgaat atgattttgt tatctactca 19680

cagactgcgg aaacagcgca ttctgtcaat gtaaatagat tcaatgttgc tattacacgt 19740

gctaagaagg gtattctctg tgtcatgagt agtatgcaat tatttgagtc tcttaatttt 19800

actacactga cgttggataa gattaacaat ccacgattac agtgtactac aaatttgttt 19860

aaggattgta gcaggagcta tgtaggatat cacccagccc atgcaccatc ctttttggca 19920

gttgatgaca aatataaggt aggcggtgat ttagccgttt gccttaatgt tgctgattct 19980

gctgtcactt attcgcggct tatatcactc atgggattca agcttgactt gacccttgat 20040

ggttattgta agctgtttat aactagagat gaagctatca aacgtgttag agcctgggtt 20100

ggcttcgatg cagaaggtgc ccatgcgata cgtgatagca ttgggacaaa tttcccatta 20160

caattaggct tttcgactgg aattgatttt gttgtcgaag ccactggaat gtttgctgag 20220

agagatggtt atgtctttaa aaaggcagcc gcacgagctc ctcctggcga acaatttaaa 20280

caccttatcc cacttatgtc aagagggcag aaatgggatg tggttcgcat tagaatagta 20340

caaatgttgt cagaccacct agtggatttg gcagacagtg ttgtacttgt gacgtgggct 20400

gccagctttg agctcacatg tttgcgatat ttcgctaaag ttggaagaga agttgtgtgt 20460

agtgtctgca ccaagcgtgc gacatgtttt aattctagaa ctggatacta tggatgctgg 20520

cgacatagtt attcctgtga ttacctgtac aacccactaa tagttgacat tcaacagtgg 20580

ggatatacag gatctttaac tagcaatcat gatcctattt gcagcgtgca taagggtgct 20640

catgttgcat catctgatgc tatcatgacc cggtgtctag ctgttcatga ttgcttttgt 20700

aagtctgtta attggaattt agaatacccc attatttcaa atgaggtcag tgttaatacc 20760

tcctgcaggt tattgcagcg cgtaatgttt agggctgcga tgctatgcaa taggtatgat 20820

gtgtgttatg acattggcaa ccctaaaggt cttgcctgtg tcaaaggata tgattttaag 20880

ttctatgacg cctcccctgt tgttaagtcg gtcaaacagt ttgtttacaa atacgaggca 20940

cataaagatc aatttttaga tggtttgtgt atgttttgga actgcaatgt ggataagtat 21000

ccagcgaatg cagttgtgtg taggtttgac acgcgtgtgt tgaacaaatt aaatctccct 21060

ggctgtaatg gtggcagttt gtatgttaac aaacatgcat tccacaccag tccctttacc 21120

cgggctgcct tcgagaattt gaagcctatg cctttctttt attattcaga tacgccctgt 21180

gtgtatatgg aaggcatgga atctaagcag gtcgattatg tcccattgag aagcgctaca 21240

tgcatcacaa gatgcaattt aggtggcgct gtttgtttaa aacatgctga ggagtatcgt 21300

gagtaccttg agtcttacaa tacggcaacc acagcgggtt ttactttttg ggtctataag 21360

acttttgatt tttacaacct ttggaatact tttactaggc tccaaagttt agaaaatgta 21420

gtgtataacc tggtcaacgc tggacacttt gatggccggg cgggtgaact gccttgtgct 21480

gttataggtg agaaagtcat tgccaagatt caaaatgagg atgtcgtggt ctttaaaaat 21540

aacacgccat tccccactaa tgtggctgtc gaattatttg ctaagcgcag tattcggccc 21600

caccccgagc ttaagctctt tagaaatttg aatattgacg tgtgctggag tcacgtcctt 21660

tgggattatg ctaaggatag tgtgttttgc agttcgacgt ataaggtctg caaatacaca 21720

gatttacagt gcattgaaag cttgaatgta ctttttgatg gtcgtgataa tggtgctctt 21780

gaagctttta agaagtgccg gaatggcgtc tacattaaca cgacaaaaat taaaagtctg 21840

tcgatgatta aaggcccaca acgtgccgat ttgaatggcg tagttgtgga gaaagttgga 21900

gattctgatg tggaattttg gtttgctgtg cgtaaagacg gtgacgatgt tatcttcagc 21960

cgtacaggga gccttgaacc gagccattac cggagcccac aaggtaatcc gggtggtaat 22020

cgcgtgggtg atctcagcgg taatgaagct ctagcgcgtg gcactatctt tactcaaagc 22080

agattattat cttctttcac acctcgatca gagatggaga aagattttat ggatttagat 22140

gatgatgtgt tcattgcaaa atatagttta caggactacg cgtttgaaca cgttgtttat 22200

ggtagtttta accagaagat tattggaggt ttgcatttgc ttattggctt agcccgtagg 22260

cagcaaaaat ccaatctggt aattcaagag ttcgtgacat acgactctag cattcattcg 22320

tactttatca ctgacgagaa cagtggtagt agtaagagtg tgtgcactgt tattgattta 22380

ttgttagatg attttgtgga cattgtaaag tccctgaatc taaagtgtgt gagtaaggtt 22440

gttaatgtta atgtggattt taaggacttc cagtttatgt tgtggtgcaa tgaggagaag 22500

gtcatgactt tctatcctcg tttgcaggct gctgctgact ggaaacctgg ttatgttatg 22560

cctgtcttat ataagtattt ggaatcgcct ctggaaagag taaacctctg gaattatggc 22620

aagccgatta ctttacctac aggatgtatg atgaatgttg ctaagtatac tcaattatgt 22680

caatatttga gcactacaac attagcagtt ccggctaata tgcgtgtctt acaccttggt 22740

gccggttcgg ataagggtgt tgcccctggg tctgcagttc ttaggcagtg gctaccagcg 22800

ggaagtattc ttgtagataa tgatgtgaat ccatttgtga gtgacagtgt cgcctcatat 22860

tatggaaatt gtataacctt accctttgat tgtcagtggg atctgataat ttctgatatg 22920

tacgaccctc ttactaagaa cattggggag tacaacgtga gtaaagatgg attctttact 22980

tacctctgtc atttaattcg tgacaagttg gctctgggtg gcagtgttgc cataaaaata 23040

acagagtttt cttggaacgc tgagttatat agtttaatgg ggaagtttgc gttctggaca 23100

atcttttgca ccaacgtaaa cgcctcttca agtgaaggat ttttgattgg cataaattgg 23160

ttgaataaga cccgtaccga aattgacggt aaaaccatgc atgccaatta tctgttttgg 23220

agaaatagta caatgtggaa tggaggggct tacagtctct ttgacatgag taagttccct 23280

ttgaaagcgg ctggtacggc tgttgttagc cttaaaccag accaaataaa tgacttagtc 23340

ctctccttga ttgagaaggg caagttatta gtgcgtgata cacgcaaaga agtttttgtt 23400

ggcgatagcc tagtaaatgt caaataaacg aacaatgttt gtttttcttg ttttattgcc 23460

actagtctct agtcagtgtg ttaatcttac aaccagaact caattacccc ctgcatacac 23520

taattctttc acacgtggtg tttattaccc tgacaaagtt ttcagatcct cagttttaca 23580

ttcaactcag gacttgttct tacctttctt ttccaatgtt acttggttcc atgctataca 23640

tgtctctggg accaatggta ctaagaggtt tgataaccct gtcctaccat ttaatgatgg 23700

tgtttacttt gcttccactg agaagtctaa cataataaga ggctggattt ttggtactac 23760

tttagattcg aaaacccagt ccctacttat tgttaataac gctactaatg ttgttatcaa 23820

agtctgtgaa tttcaatttt gtaacgatcc atttttgggt gtttattacc acaaaaacaa 23880

caaaagttgg atggaaagtg agttcagagt ttattctagt gcgaataatt gcacttttga 23940

atacgtctct cagccttttc ttatggacct tgaaggaaaa cagggtaatt tcaaaaatct 24000

tagggaattt gtgttcaaga atattgatgg ttacttcaag atatactcta agcacacgcc 24060

tattaattta gtgcgtgatc tccctcaggg tttttcggct ttagaaccat tggtagattt 24120

gccaataggt attaacatca ctaggtttca aactttactt gctttacata gaagttattt 24180

aactcctggt gattcttctt caggttggac agctggtgct gcagcttatt atgtgggtta 24240

tcttcaacct aggacttttc tactgaagta caatgaaaat ggaaccatta cagatgctgt 24300

agactgtgca cttgaccctc tctcagaaac aaagtgtacg ttgaaatcct tcactgtaga 24360

aaaaggaatc tatcaaactt ctaactttag agtccaacca acagaatcta ttgttagatt 24420

tcctaacatc acaaacttgt gcccttttgg tgaagttttt aacgccacca gatttgcatc 24480

tgtttatgct tggaacagga agagaatcag caactgtgtt gctgattatt ctgtcctgta 24540

taattccgca tcattttcca cttttaagtg ttatggagtg tctcctacta aattaaatga 24600

tctctgcttt actaatgtct atgcagattc atttgtaatt agaggtgatg aagtcagaca 24660

aatcgctcca gggcaaactg gaaagattgc tgattataac tacaaattac cagatgattt 24720

tacaggctgc gttatagctt ggaattctaa caatcttgat tctaaggttg gtggtaatta 24780

taattacctg tacagattgt ttaggaagtc taatctcaaa ccttttgaga gagatatttc 24840

aactgaaatc tatcaggccg gtagcacacc ttgtaatggt gttgaaggtt ttaattgtta 24900

ctttcctctg caatcatatg gtttccaacc cactaatggt gttggttacc aaccatacag 24960

agtagtagta ctttcttttg aacttctaca tgcaccagca actgtttgtg gacctaaaaa 25020

gtctactaat ttggttaaga acaagtgtgt caatttcaac ttcaatggtt taacaggcac 25080

aggtgttctt actgagtcta acaaaaagtt tctgcctttc caacaatttg gcagagacat 25140

tgctgacact actgatgctg ttcgtgatcc acaaacactt gagattcttg acattacacc 25200

atgttctttt ggtggtgtca gtgttataac accaggaaca aatacttcta accaggttgc 25260

tgttctttat caggatgtta actgcacaga agtccctgtt gctattcatg cagatcaact 25320

tactcctact tggcgtgttt attctacagg ttctaatgtt tttcaaacac gtgcaggctg 25380

tttaataggg gctgaacatg tcaacaactc atatgagtgt gacataccca ttggtgcagg 25440

tatatgcgct agttatcaga ctcagactaa ttctcctcgg agagcaagaa gtgtagctag 25500

tcaatccatc attgcctaca ctatgtcact tggtgcagaa aattcagttg cttactctaa 25560

taactctatt gccataccca caaattttac tattagcgtt accacagaaa ttctaccagt 25620

gtctatgacc aagacatcag tagattgtac aatgtacatt tgtggtgatt caactgaatg 25680

cagcaatctt ttgttgcaat atggcagttt ttgtacacaa ttaaaccgtg ctttaactgg 25740

aatagctgtt gaacaagaca aaaacaccca agaagttttt gcacaagtca aacaaattta 25800

caagacacca ccaattaaag attttggcgg ttttaatttt agccagatac tgccagatcc 25860

atcaaaacca agcaagaggt catttattga agatctactg ttcaacaaag tgacacttgc 25920

agatgctggc ttcatcaaac aatatggtga ttgccttggt gatattgctg ctagagacct 25980

catttgtgca caaaagttta acggccttac tgttttgcca cctttgctca cagatgaaat 26040

gattgctcaa tacacttctg cactgttagc aggtacaatc acttctggtt ggacttttgg 26100

tgcaggtgct gcattacaaa taccatttgc tatgcaaatg gcttataggt ttaatggtat 26160

tggagttaca cagaatgttc tctatgagaa ccaaaaattg attgccaacc aatttaatag 26220

tgctattggc aaaattcaag actcactttc ttccacagca agtgcacttg gaaaacttca 26280

agatgtggtc aaccaaaatg cacaagcttt aaacacgctt gttaaacaac ttagctccaa 26340

ttttggtgca atttcaagtg ttttaaacga catcctttca cgtcttgaca aagttgaggc 26400

tgaagtgcaa attgataggt tgatcacagg cagacttcaa agtttgcaga catatgtgac 26460

tcaacaatta attagagctg cagaaatcag agcttctgct aatcttgctg ctactaaaat 26520

gtcagagtgt gtacttggac aatcaaaaag agttgacttt tgcggaaagg gctatcatct 26580

tatgtcattt cctcagtcag cacctcatgg tgtcgtcttt ttgcatgtga cttatgtccc 26640

tgcacaagaa aagaacttca caactgctcc tgccatttgt catgatggaa aagcacactt 26700

tcctcgtgaa ggtgtctttg tttcaaatgg cacacactgg tttgtaacac aaaggaattt 26760

ttatgaacca caaatcatta ctacagacaa cacatttgtg tctggtaact gtgatgttgt 26820

aataggaatt gtcaacaaca cagtttatga tcctttgcaa cctgaattag actcattcaa 26880

ggaggagctt gataaatact tcaagaacca tacctcacca gatgttgatt taggtgacat 26940

ctctggcatt aatgcttcag ttgtaaacat tcagaaagaa atcgaccgcc tcaatgaggt 27000

tgccaagaat ttaaatgaat ctctcatcga tctccaagaa cttggaaagt atgagcagta 27060

tataaaatgg ccatggtaca tttggctagg ttttatagct ggcttgattg ccatagtaat 27120

ggtgacaatt atgctttgct gtatgaccag ttgctgtagt tgtctcaagg gctgttgttc 27180

ttgtggatcc tgctgcaaat ttgacgagga cgactctgag ccagtgctca aaggagtcaa 27240

attacattac acataactat cacagcctct cctggaaaga cagaaaatct aaacaattta 27300

tagcattctc attgctacct ggccccgtaa gaggcagtca tagctatggc cgtgttggtc 27360

ctaaggctac attggctgct gtctttattg gtccatttat tgtagcatgt atgctaggca 27420

ttggcctagt ttatttattg caattgcaag ttcaaatttt tcatgttaag gataccatac 27480

gtgtgactgg caagccagcc actgtgtctt atactacaag tacaccagta acaccgagcg 27540

cgacgacgct cgatggtact acgtatactt taattagacc cactagctct tatacaagag 27600

tttatcttgg tactccaaga ggttttgatt atagtacatt tgggcctaag accctagatt 27660

atgttactaa tctaaacctc atcttaattc tggtcgtcca tatactttta aggcattgtc 27720

caggcatatg aggccaacag ccacatggat ttggcatgtg agtgatgcat ggttacgccg 27780

cacgcgggac tttggtgtca ttcgcctaga agatttttgt tttcaattta attatagcca 27840

accccgagtt ggttattgta gagttccttt aaaggcttgg tgtagcaacc agggtaaatt 27900

tgcagcgcag tttaccctaa aaagttgcga aaaaccaggt cacgaaaaat ttattactag 27960

cttcacggcc tacggcagaa ctgtccaaca ggccgttagc aagttagtag aagaagctgt 28020

tgattttatt ctttttaggg ccacgcagct cgaaagaaat gtttaattta ttccttacag 28080

acacagtatg gtatgtgggg cagattattt ttatattcgc agtgtgtttg atggtcacca 28140

taattgtggt tgccttcctt gcgtctatca aactttgtat tcaactttgc ggtttatgta 28200

atactttggt gctgtcccct tctatttatt tgtatgatag gagtaagcag ctttataagt 28260

actataatga agaaatgaga ctgcccctat tagaggtgga tgatatctaa tccaaacatt 28320

atgagtagta ctactcaggc cccagagccc gtctatcaat ggaccgccga cgaggcagtt 28380

caattcctta aggaatggaa cttctcgttg ggcattatac tactctttat tactatcata 28440

ctacagttcg gttacacgag ccgtagcatg tttatttatg ttgtgaaaat gataatcttg 28500

tggttaatgt ggccactgac tattgttttg tgtattttca attgcgtgta tgcgctaaat 28560

aatgtgtatc ttggattttc tatagtgttt actatagtgt ccattgtaat ctggatcatg 28620

tattttgtga acagcataag gttgtttatc aggactggta gctggtggag cttcaacccc 28680

gaaacaaaca accttatgtg tatagatatg aaaggtaccg tgtatgttag acccattatt 28740

gaggattacc atacactaac agccactatt attcgtggcc acctctacat gcaaggtgtt 28800

aagctaggca ccggtttctc tttgtctgac ttgcccgctt atgttacagt tgctaaggtg 28860

tcacaccttt gcacttataa gcgcgcattc ttagacaagg tagacggtgt tagcggtttt 28920

gctgtttatg tgaagtccaa ggtcggaaat taccgactgc cctcaaacaa accgagtggc 28980

gcggacaccg cattgttgag aacctaatct aaactttaag gagagaatga atcctatgtc 29040

ggcgctcggt ggtaacccct cgcgagaaag tcgggatagg acactctcta tcagaatgga 29100

tgtcttgctg tcataacaga tagagaaggt tgtggcagac cctgtatcaa ttagttgaaa 29160

gagattgcaa aatagagaat gtgtgagaga agttagcaag gtcctacgtc taaccataag 29220

aacggcgata ggcgccccct gggaacagct cacatcaggg tactattcct gcaatgccct 29280

agtaaatgaa tgaagttgat catggccaat tggaagaatc acaaaaaaaa aaaaaaaaaa 29340

aacggccggt ttaaacgcta cagtccaagt tccaagcggg atactagatg tataatgtcc 29400

gccatgcaga cgaaaccagt cggagattac cgagcattct atcacgtcgg cgaccaatag 29460

tgagcttagg gataacaggg taataaacga tccccgggaa ttcactggcc gtcgttttac 29520

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 29580

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 29640

gcagcctgaa tggcgaatgg cgatagatcc ggtggatgac cttttgaatg acctttaata 29700

gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt aaaaattttt 29760

tcacaaaacg gtttacaagc ataaagctcg gacggatctt ttccgctgca taaccctgct 29820

tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat 29880

tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga 29940

taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc 30000

tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag 30060

atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca 30120

aggtgtcgat gcaggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat 30180

tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 30240

tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa 30300

ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 30360

aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 30420

tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 30480

ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 30540

actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 30600

aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 30660

ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 30720

gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 30780

acaagtctgg aaagaaatgc ataagttttt gccattctca ccggattcag tcgtcactca 30840

tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 30900

tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 30960

cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 31020

tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 31080

taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga 31140

ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac 31200

cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc 31260

atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag 31320

tcagcaacac cttcttcacg aggcagacct cagacggtat cggatcgatc ccccgatgtg 31380

tagcagtggc ggaccatata ggcagatcag aaggcgcggt tctcctacat gagcttttca 31440

attcaattca tcattttttt tttattcttt tttttgattt cggtttcctt gaaatttttt 31500

tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt 31560

atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact 31620

gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga 31680

acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa 31740

gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt 31800

tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt 31860

ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact 31920

cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg 31980

tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg 32040

tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt 32100

gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac 32160

tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat 32220

gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga 32280

caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc 32340

tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg 32400

tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta 32460

aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta 32520

attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa 32580

aaaaattgga aagaaaaagc tgggcgcgcc ggccggccct tttcatcacg tgctataaaa 32640

ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa 32700

gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc 32760

caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt 32820

catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg 32880

ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt 32940

acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttttcttc attccgtaac 33000

tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac 33060

acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag 33120

gcaagcgatc ggccggcccg ggcatttaaa tgcaggccgc gtacgcgtcg acggtaccga 33180

attcgcttaa acgagctcat gttcgccggt gaacgcgttg aggaagccgg gcagtgcctc 33240

ggcaaaatcc ttgcgtgtag acaagacatc tgcgtagcag ttgtcctcaa caacgatgtc 33300

gaaatccaaa tcggagtgct catcgagtcc tccgtgaacg taagagccgc cgatcagaag 33360

agcgcggaag cgaacatcgg aagcgaccgc atcgcggatg cggttcaaga aagttgcatg 33420

agcttgtgga agtgtgctga gcataaatga ttctcctagc tgttctttgg gtaagtacgc 33480

catcaggacg ttgtgagtgg cgcgattttt agcggctgaa atcagccctt gagcctgtcg 33540

gcaagtcgcg tcatgaggtc catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg 33600

cccgcacgcg gcaggcaaaa aacgtagtgg tgttcgcagc gggccatccg cagcgcggga 33660

aagagttcgc tcatgtcctt aaacgggcct tcgccggcgg caagcctggc tatgccctgt 33720

tccagcttag cgatatagcg gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg 33780

atgatgccgc gtagatcggc ttcggcctca gccgtgagga tgtaggccgt caagcgcgat 33840

ccccgctgag ttcttcatca agaatttcgc cgacgctctt ggtggacacc ttgccggcaa 33900

gcccatcgtt gatgcggttc cccagcatgg ttttcagttc ctgccatgcc tgatcggcat 33960

cagcgtcacc ggggaacaga cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg 34020

cggccagggc tttcaggctc tggtgctgct ggtccgtcat gtcgattgtc aggcggctca 34080

ttggataacc tccataaaat acacgtaacc acattagcac atatgtgggc gtgaggctac 34140

agcgcgaggc gcattaaggt cgggaaaatg cgctaggcgc atttaaattg cgtattgctg 34200

taatgcgcca tgccggctag actaggccca aatgggtata cccaatttga ccaaggggga 34260

cgcgatgagg gcggccaagc actaccgaca acttctatcc atcgacttca acatcgaggc 34320

gctggccttc gtgcctggac ccgacggcac acgcggccgg cgcatccacg tcctggggcg 34380

cgaggtccgc gaccggcccg gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt 34440

ggcgctggac ggctactgca aggccaattt cgatgcagtg ctgcacctgg cgtaccccga 34500

tcatcagcaa tggggccacg catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc 34560

gcgctggccg tcctggtcgt tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg 34620

cgcatcatcg acggcgacac catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc 34680

ctggtggaca ttgacgcgcc ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg 34740

ctggccggca tggtgttccg ccggcacgtc ctggtcgacg agaaggacac cgaccgttac 34800

ggccgcacgc tgggcaccgt gtgggtcaac atggagctgg ccagccggcc gccgcagccg 34860

cgcaacgtca acgccgcgat ggttcaccag ggcatggcgt gggcctatcg cttccacggc 34920

cgcgcggccg accctgaaat gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc 34980

ctctggtccg atccgcacgc cgtcgagccg tggaaatggc gacgcgagag caacaaccgg 35040

agggacgaag gttgaaggtc gcccgcatct acctgcgcgc cagtacggac gagcagaatc 35100

ttgaacgcca ggagagcctt gtagcggcca cgcgggccgc cgggtactac gtcgccggca 35160

tctaccgcga gaaggcgtcc ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg 35220

cggacctgca acctggtgaa gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc 35280

cgttggccga ggccgagcgc ctggttgcgt cgatccgggc caaaggggcc aagctggccg 35340

tgcctggcgt ggtggacctg tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg 35400

ttctggaatc cgtccaggac atgcttttga agctcgcctt gcagatggcc cgcgacgact 35460

acgaggatcg gcgcgagcgt caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct 35520

acaccggccg caaacgtgac gccggcatgc acgaccgcat catcacgctt cgctccggcg 35580

gatcgagcat tgccaagacg gccaagctgg tcggatgcag cccgagccag gtcaaacgag 35640

tgtgggcggc ctggaacgcg cagcagcaaa aataaagccg ggcagtgccc ggcttttctc 35700

accttttcgc gtcccgcagg gccgctgcga gcgccctacc tagatcctcg ctttccccct 35760

cggtgtagtc cggccagggc acgaagggcg cggatgcgaa cctgttgagc aggtacgcct 35820

tcgggcagcg gtagaccacc ggcgagttcg ccttttcatc ccaccgggcc aggatcacgt 35880

ccgcatcgca gtgcatgtcc ttcacctggt cgcggaagaa gccgaaggcc accatgccgc 35940

tatgttcgcc gaggaacgcc agttgcttcg cgctggcgat cgcgccgacg ccgccggcca 36000

aaaccgacgc catcacccag ccgacgaacc agaagctggc atgcttgcgg ttgaccaccg 36060

cacgcgcagc cgcgaccagg acaacggcca agctgccgac cagggccatg acgaccgtga 36120

tccggccgtt gtggaaagcg atgggcttgc cagcgtccgc ttgcacggcg tcgtaaatgc 36180

tggacccgat gggcgcgcac atcagcacga caggcagcag caccaggaac atcgtccgcg 36240

tccattgcgc gagtgccttg cggcgttcgc cggcggcaag cgcctccatc atcggcgtga 36300

agcccaacag ggccaccgca gccgccaagc cggcaacgat gccgcaggcg attacataca 36360

tacatcctcc ctaatgcgcc ttgcgcacgg ttgtagtcag agtccgcggt ggggcgataa 36420

gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 36480

aaagatcaaa ggatcttctt gagatccttt ttttctgcgg gggatcagga ccgctgccgg 36540

agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc ctttccaccg 36600

cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta gcgtccaagc 36660

ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcctgctgcg gcgtccgctc 36720

gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc tggcgctcgc gggcgaggtc 36780

cagggcggcc gtcttcacgt tctgccttgc gcagatgaga tagatcgatc tagcgtggac 36840

tcaaggctct cgcgaatggc tcgcgttgga aactttcatt gacacttgag gggcaccgca 36900

gggaaattct cgtccttgcg agaaccggct atgtcgtgct gcgcatcgag cctgcgccct 36960

tggcttgtct cgcccctctc cgcgtcgcta cggggcttcc agcgcctttc cgacgctcac 37020

cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga 37080

aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga 37140

aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc 37200

cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc 37260

agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac 37320

aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg 37380

aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt 37440

gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt 37500

tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt 37560

ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc 37620

ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct 37680

cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga 37740

tcggggcagt aacgggatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc 37800

cgcaggtgct ggcatcgaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg 37860

gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg 37920

caatttttac cttgggcatt cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg 37980

gtgaattaat tccccggatc gatccgtcag cttcacgctg ccgcaagcac tcagggcgca 38040

agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc 38100

ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc 38160

aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa 38220

gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa 38280

actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tcgacggatc 38340

gatccgggga attaattccg gggcaatccc gcaaggaggg tga 38383

<210> 41

<211> 29494

<212> DNA

<213> Artificial sequence

<220>

<223> Synthesis of optimized sequence E-protein and ORF6 double deletion

<400> 41

caaatataac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 60

taatacgact cactataggg attaaaggtt tataccttcc caggtaacaa accaaccaac 120

tttcgatctc ttgtagatct gttctctaaa cgaactttaa aatctgtgtg gctgtcactc 180

ggctgcatgc ttagtgcact cacgcagtat aattaataac taattactgt cgttgacagg 240

acacgagtaa ctcgtctatc ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga 300

tcatcagcac atctaggttt cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc 360

cctggtttca acgagaaaac acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac 420

gtgttagtac gtggttttgg agattcagtg gaagaagtct tatcagaggc acgtcaacat 480

cttaaagatg gcacttgtgg cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa 540

cagccctatg tgttcatcaa acgttctgat gctagaactg cacctcatgg tcatgttatg 600

gttgagctgg tagcagaatt agaaggtatt cagtacggtc gtagtggtga gacattaggt 660

gttttagttc ctcatgtggg cgaaatacca gtggcttacc gcaaagttct tcttagaaag 720

aacggtaata aaggagctgg tggccatagt tacggcgctg atttaaagtc atttgactta 780

ggcgacgagc ttggcactga tccttatgaa gatttccaag aaaactggaa cactaaacat 840

agcagtggtg ttacccgtga actcatgcgt gagttaaatg gaggtgcata cactcgctat 900

gtcgataaca acttctgtgg acctgatggt taccctcttg agtgcattaa agaccttcta 960

gcacgtgctg gtaaagcttc atgcactttg tccgaacaac tggactttat tgacactaag 1020

aggggtgtat actgctgccg tgaacatgag catgaaattg cttggtacac ggaacgttct 1080

gaaaagagct atgaattgca gacacctttt gaaattaaac tggcaaagaa atttgacacc 1140

ttcaatgggg aatgtccaaa ttttgtattt cccctcaatt ccataatcaa gactattcaa 1200

ccaagggttg aaaagaaaaa gcttgatggc tttatgggta gaattcgatc tgtctatcca 1260

gttgcgtcac caaatgaatg caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat 1320

tgtggtgaaa cttcatggca gacgggcgat tttgttaaag ccacttgcga attttgtggc 1380

actgagaatt tgactaaaga aggtgccact acttgtggtt acttacccca aaatgctgtt 1440

gttaaaattt actgtccagc atgtcacaat tcagaagtag gacctgagca tagtcttgcc 1500

gaataccata atgaatctgg cttgaaaacc attcttcgta agggtggtcg cactattgct 1560

tttggaggat gtgtgttctc ttatgttggt tgccataaca agtgtgctta ttgggttcca 1620

cgtgcttcag ctaacatagg ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt 1680

cttaatgaca accttcttga aatactccaa aaagagaaag tcaacatcaa tattgttggt 1740

gactttaaac ttaatgaaga gatcgccatt attttggcat ctttttctgc ttccacaagt 1800

gcttttgtgg aaactgtgaa aggtttggat tataaagcat tcaaacagat tgttgaatcc 1860

tgtggtaatt ttaaggttac aaagggaaaa gctaaaaaag gtgcctggaa tattggtgaa 1920

cagaaatcaa tactgagtcc tctttatgca tttgcatcag aggctgctcg tgttgtacga 1980

tcaattttct cccgcactct tgaaactgct caaaattctg tgcgtgtttt acagaaggcc 2040

gctataacaa tactagatgg aatttcacag tattcactga gactcattga tgctatgatg 2100

ttcacatctg atttggctac taacaatcta gttgtaatgg cctacattac aggtggtgtt 2160

gttcagttga cttcgcagtg gctaactaac atctttggca ctgtttatga aaaactcaaa 2220

cccgtccttg attggcttga agagaagttt aaggaaggtg tagagtttct tagagacggt 2280

tgggagattg ttaaattcat ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc 2340

acctgtgcta aggaaattaa ggagagtgtt cagacattct ttaagcttgt aaacaagttt 2400

ttggctttgt gtgctgactc tatcattatt ggtggagcta aacttaaagc cttgaattta 2460

ggtgaaacat ttgtcacgca ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa 2520

gaaactggcc tactcatgcc tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa 2580

acacttccca cagaagtgtt aacagaggaa gttgtcttga aaactggtga tttacaacca 2640

ttagaacaac ctactagtga agctgttgaa gctccattgg ttggtacacc agtttgtatt 2700

aacgggctta tgttgctcga aatcaaagac acagaaaagt actgtgccct tgcacctaat 2760

atgatggtaa caaacaatac cttcacactc aaaggcggtg caccaacaaa ggttactttt 2820

ggtgatgaca ctgtgataga agtgcaaggt tacaagagtg tgaatatcac ttttgaactt 2880

gatgaaagga ttgataaagt acttaatgag aagtgctctg cctatacagt tgaactcggt 2940

acagaagtaa atgagttcgc ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca 3000

gtatctgaat tacttacacc actgggcatt gatttagatg agtggagtat ggctacatac 3060

tacttatttg atgagtctgg tgagtttaaa ttggcttcac atatgtattg ttctttctac 3120

cctccagatg aggatgaaga agaaggtgat tgtgaagaag aagagtttga gccatcaact 3180

caatatgagt atggtactga agatgattac caaggtaaac ctttggaatt tggtgccact 3240

tctgctgctt tacaacctga agaagaacaa gaagaagatt ggttagatga tgatagtcaa 3300

caaactgttg gtcaacaaga cggcagtgag gacaatcaga caactactat tcaaacaatt 3360

gttgaggttc aacctcaatt agagatggaa cttacaccag ttgttcagac tattgaagtg 3420

aatagtttta gtggttatct taaacttact gacaatgtat acatcaagaa tgcagacatt 3480

gtggaagaag ctaaaaaggt aaaaccaaca gtggttgtta atgcagccaa tgtttacctt 3540

aaacatggag gaggtgttgc aggagcctta aataaggcta ctaacaatgc catgcaagtt 3600

gaatctgatg attacatagc tactaatgga ccacttaaag tgggtggtag ttgtgtttta 3660

agcggacaca atcttgctaa acactgttta catgttgtcg gcccaaatgt taacaaaggt 3720

gaagatattc aacttcttaa gagtgcttat gaaaatttta accagcacga agttctactt 3780

gcaccattat tatcagctgg tatttttggt gctgacccta tacattcttt aagagtttgt 3840

gtagatactg ttcgcacaaa tgtctactta gctgtctttg ataaaaatct ctatgacaaa 3900

cttgtttcaa gctttttgga aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag 3960

attcctaaag aggaagttaa gccatttata actgaaagta aaccttcagt tgaacagaga 4020

aaacaagatg ataagaagat caaagcttgt gttgaagaag ttacaacaac tctggaagaa 4080

actaagttcc tcacagaaaa cttgctcctt tatatcgaca ttaatggcaa tcttcatcca 4140

gattctgcca ctcttgttag tgacattgac atcactttct taaagaaaga tgctccatat 4200

atagtgggtg atgttgttca agagggtgtt ttaactgctg tggttatacc tactaaaaag 4260

gctggtggca ctactgaaat gctagcgaaa gctttgagaa aagtgccaac agacaattat 4320

ataaccactt acccgggtca gggtttaaat ggttacactg tagaggaggc aaagacagtg 4380

cttaaaaagt gtaaaagtgc cttttacatt ctaccatcta ttatctctaa tgagaagcaa 4440

gaaattcttg gaactgtttc ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca 4500

cgcaaattaa tgcctgtctg tgtggaaact aaagccatag tttcaactat acagcgtaaa 4560

tataagggta tcaagataca agagggtgtg gttgattatg gtgctagatt ttacttttac 4620

accagtaaaa caactgtagc gtcacttatc aacacactta acgatctaaa tgaaactctt 4680

gttacaatgc cacttggcta tgtaacacat ggcttaaatt tggaagaagc tgctcggtat 4740

atgagatctc tcaaagtgcc agctacagtt tctgtttctt cacctgatgc tgttacagcg 4800

tataatggtt atcttacttc ttcttctaaa acacctgaag aacattttat tgaaaccatc 4860

tcacttgctg gttcctataa agattggtcc tattctggac aatctacaca actaggtata 4920

gaatttctta agagaggtga taaaagtgta tattacacgt ccaatcctac cacattccac 4980

ctagatggtg aagttatcac ctttgacaat cttaagacac ttctttcttt gagagaagtg 5040

aggactatta aggtgtttac aacagtagac aacattaacc tccacacgca agttgtggac 5100

atgtcaatga catatggaca acagtttggt ccaacttatt tggatggagc tgatgttact 5160

aagataaaac ctcataactc acatgaaggt aaaacatttt acgttttgcc taatgatgac 5220

actctacgtg ttgaggcttt tgagtactac cacacaactg atcctagttt tctgggtagg 5280

tacatgtcag cattaaatca cactaaaaag tggaaatacc cacaagttaa tggtttaact 5340

tcgattaaat gggcagataa caactgttat cttgccactg cattgttaac actccaacaa 5400

atagagttga agtttaatcc acctgctcta caagatgctt attacagagc aagggctggt 5460

gaagctgcta acttttgtgc acttatctta gcctactgta ataagacagt aggtgagtta 5520

ggtgatgtta gagaaacaat gagttacttg tttcaacatg ccaatttaga ttcttgcaaa 5580

agagtcttga acgtggtgtg taaaacttgt ggacaacagc agacaaccct taagggtgta 5640

gaagctgtta tgtacatggg cacactttct tatgaacaat tcaagaaagg tgttcagata 5700

ccttgtacgt gtggtaaaca agctacaaaa tatctagtac aacaggagtc accttttgtt 5760

atgatgtcag caccacctgc tcagtatgaa cttaagcatg gtacatttac ttgtgctagt 5820

gagtacactg gtaattacca gtgtggtcac tataagcata taacttctaa ggaaactttg 5880

tattgcatag acggtgcttt acttacaaag tcctcagaat acaaaggtcc tattacggat 5940

gttttctaca aagaaaacag ttacacaaca accataaaac cagttactta taagttggat 6000

ggtgttgttt gtacagaaat tgaccctaag ttggacaatt attataagaa ggacaactct 6060

tatttcacag agcaaccaat tgatcttgta ccaaaccaac catatccaaa cgcaagcttc 6120

gataatttta agttcgtatg cgataatatc aaatttgctg atgatctcaa ccagttaact 6180

ggttataaga aacctgcttc aagagagctt aaagttacat ttttccctga cttaaatggt 6240

gatgtggtgg ctattgatta taaacactac acaccctctt ttaagaaagg agctaaattg 6300

ttacataagc ctattgtttg gcatgttaac aatgcaacta ataaagccac gtataaacca 6360

aatacctggt gtatacgttg tctttggagc acaaaaccag ttgaaacatc aaattcgttt 6420

gatgtactga agtcagagga cgcgcaggga atggataatc ttgcatgtga agatctaaaa 6480

ccagtctctg aagaagtagt ggaaaatcct accatacaga aagacgttct tgagtgtaat 6540

gtgaaaacta ccgaagttgt aggagacatt atacttaaac cagcaaataa tagtttgaag 6600

atcacagaag aggttggcca cacagatcta atggctgctt atgtagacaa ttctagtctt 6660

actattaaga aacctaatga actctctaga gtattaggtt tgaaaaccct tgctactcat 6720

ggtttagctg ctgttaatag tgtcccttgg gatactatag ctaattatgc taagcctttt 6780

cttaacaaag ttgttagtac aactactaac atagttacac ggtgtcttaa tcgtgtttgt 6840

actaattata tgccttactt ctttacttta ttgctacaat tgtgtacttt tactagaagt 6900

acaaattcta gaatcaaggc atctatgccg actactatag caaagaatac tgttaagagt 6960

gtcggtaaat tttgtctaga ggcttcattt aattatctca agtcacctaa cttttctaag 7020

ctgataaaca ttatcatctg gtttttgcta ttaagtgttt gcctaggttc tttaatctac 7080

tcaaccgctg ctttaggtgt tttaatgtct aatttaggca tgccttctta ctgtactggt 7140

tacagagaag gctatttgaa ctctactaat gtcactattg caacctactg tactggatct 7200

ataccttgta gtgtttgtct tagtggttta gattctttag acacctatcc ttctcttgaa 7260

actatacaga ttaccatttc atctttcaaa tgggatttaa ctgcttttgg cttagttgca 7320

gagtggtttt tggcatatat tcttttcact aggtttttct atgtacttgg attggctgca 7380

atcatgcaat tgtttttcag ctattttgca gtccatttta ttagtaactc ttggcttatg 7440

tggcttataa ttaatcttgt gcagatggcc ccgatttcag ctatggttag aatgtacatc 7500

ttctttgcct cattttatta tgtgtggaaa agttatgtgc atgttgtaga cggttgtaat 7560

tcatcaactt gtatgatgtg ttacaaacgt aatagagcaa caagagtcga atgtacaact 7620

attgttaatg gtgttagaag gtccttttat gtctatgcta atggaggtaa aggcttttgc 7680

aaactacaca attggaattg tgttaattgt gatacattct gtgctggtag tacatttatt 7740

agtgatgaag ttgcgagaga cttgtcacta cagtttaaaa gaccaataaa tcctactgac 7800

caatcttctt acatcgttga tagtgttaca gtgaagaatg gttccatcca tctttacttt 7860

gataaagctg gtcaaaagac ttatgaaaga cattctctct ctcattttgt taacttagac 7920

aacctgagag ctaataacac taaaggttca ttgcctatta atgttatcgt tttcgacggt 7980

aaatcaaaat gtgaagaatc atctgcaaaa tcagcgtctg tttactacag tcagcttatg 8040

tgtcaaccta tactgttact agatcaggca ttagtgtctg atgttggtga tagtgcggaa 8100

gttgcagtta aaatgtttga tgcttacgtt aatacgtttt catcaacttt taacgtacca 8160

atggaaaaac tcaaaacact agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc 8220

ttagacaatg tcttatctac gtttatttca gcagctcggc aagggtttgt tgattcagat 8280

gtagaaacta aagatgttgt tgaatgtctt aaattgtcac atcaatctga catagaagtt 8340

actggcgata gttgtaataa ctatatgctc acctataaca aagttgaaaa catgacaccc 8400

cgtgaccttg gtgcttgtat tgactgtagt gctagacata ttaatgcgca ggtagcaaaa 8460

agtcacaaca ttgctttgat atggaacgtt aaagatttca tgtcattgtc tgaacaacta 8520

cgaaaacaaa tacgtagtgc tgctaaaaag aataacttac ccttcaagtt gacatgtgca 8580

actactagac aagttgttaa tgttgtaaca acaaagatag cacttaaggg tggtaaaatt 8640

gtgaataact ggttgaagca gcttattaaa gttacacttg tgttcctttt tgttgctgct 8700

attttctatc tgataacacc tgttcatgtc atgtctaaac atactgactt ttcaagtgaa 8760

atcataggat acaaggctat tgatggtggt gtcactcgtg acatagcatc tacagatact 8820

tgttttgcta acaaacatgc tgattttgac acatggttta gccagcgtgg tggtagttat 8880

actaatgaca aagcttgccc attgattgct gcagtcataa caagagaagt gggttttgtc 8940

gttcctggtt tgcctggaac gatattacgc acaactaatg gtgacttttt gcatttctta 9000

cctagagttt ttagtgcagt tggtaacatc tgttacacac catcaaaact tatagagtac 9060

actgactttg caacatcagc ttgtgttttg gctgctgaat gtacaatttt taaagacgct 9120

tctggtaagc cagtaccata ttgttatgat accaatgtac tagaaggttc tgttgcttat 9180

gaaagtttac gccctgacac acgttatgtg ctcatggatg gctctattat tcaatttcct 9240

aacacctacc ttgaaggttc tgtaagagtg gtaacaactt ttgattctga gtactgtagg 9300

cacggcactt gtgaaagatc agaagctggt gtttgtgtat ctactagtgg tagatgggta 9360

cttaacaacg attattacag atctttacca ggagttttct gtggtgtaga tgctgtaaat 9420

ttgcttacta acatgtttac accactaatt caacctattg gtgctttgga catatcagca 9480

tctatagtag ctggtggtat tgtagctatc gtagtaacat gccttgccta ctattttatg 9540

aggtttagac gtgcttttgg tgaatacagt catgtagttg cctttaatac tctcctattc 9600

cttatgtcat tcactgtact ctgtttaaca ccagtttact cattcttacc tggtgtttat 9660

tctgttattt acctgtactt gacattttat ctgactaatg atgtttcttt tctcgcacat 9720

attcagtgga tggttatgtt cacaccttta gtacctttct ggataacaat tgcttacatc 9780

atttgtattt ccacaaagca tttctattgg ttctttagta attacctaaa gagacgtgta 9840

gtctttaatg gtgtttcctt tagtactttt gaagaagctg cgctgtgcac ctttttgtta 9900

aataaggaga tgtatctaaa gttgcgtagt gatgtgctat tacctcttac gcaatataat 9960

agatacttag ctctttataa caagtacaag tatttcagtg gagcaatgga tacaactagc 10020

tacagagaag ctgcttgttg tcatctcgca aaggctctca atgacttcag taactcaggt 10080

tctgatgttc tttaccaacc accacaaacc tctatcacct cagctgtttt gcagagtggt 10140

tttagaaaaa tggcattccc atctggtaaa gttgagggtt gtatggtaca agtaacttgt 10200

ggtacaacta cacttaacgg tctttggctt gatgacgtag tttactgtcc aagacatgtg 10260

atctgcacct ctgaagatat gcttaaccct aattatgaag atctactcat ccgtaagtct 10320

aatcataact tcttggtaca ggctggtaat gttcaactca gggttattgg acattctatg 10380

caaaattgtg tacttaagct taaggttgat acagccaatc ctaagacacc taagtataag 10440

tttgttcgca ttcaaccagg acagactttt tcagtgttag cttgttacaa tggttcacca 10500

tctggtgttt accaatgtgc tatgaggccc aatttcacta ttaagggttc attccttaat 10560

ggttcatgtg gtagtgttgg ttttaacata gattatgact gtgtctcttt ttgttacatg 10620

caccatatgg aattaccaac tggagttcat gctggcacag acttagaagg taacttttat 10680

ggaccttttg ttgacaggca aacagcacaa gcagctggta cagatacaac tattacagtt 10740

aatgttcttg cttggttgta cgctgctgtt ataaatggag acaggtggtt tctcaatcga 10800

tttaccacaa ctcttaatga ctttaacctt gtggctatga agtacaatta tgaacctcta 10860

acacaagacc atgttgacat actaggacct ctttctgctc aaactggaat tgccgtttta 10920

gatatgtgtg cttcattaaa agaacttctg caaaatggta tgaatggacg taccatattg 10980

ggtagtgctt tattagaaga tgagtttaca ccttttgatg ttgttagaca atgctcaggt 11040

gttactttcc aaagtgcagt gaaaagaaca atcaagggta cacaccactg gttgttactc 11100

acaattttga cttcactttt agttttagtc cagagtactc aatggtcttt gttctttttc 11160

ttctacgaaa atgccttttt accttttgct atgggtatta ttgctatgtc tgcttttgca 11220

atgatgtttg tcaaacataa gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc 11280

actgtagctt actttaatat ggtctacatg cctgctagtt gggtgatgcg tattatgaca 11340

tggttggata tggttgatac tagtttgtct ggttttaagc taaaagactg tgttatgtat 11400

gcatcagctg tagtgttact aatccttatg acagcaagaa ctgtgtatga tgatggtgct 11460

aggagagtgt ggacacttat gaatgtcttg acactcgttt ataaagttta ctatggcaac 11520

gctttagatc aagccatttc catgtgggct cttataatct ctgttacttc taactactca 11580

ggtgtagtta caactgtcat gtttttggcc agaggtattg tttttatgtg tgttgagtat 11640

tgccctattt tcttcataac tggtaataca cttcagtgta taatgctagt ctattgtttc 11700

ttaggctatt tttgtacttg ttacttcggc ctcttttgtt tactcaaccg ctactttaga 11760

ctgactcttg gtgtttatga ttacttagtg tctacacagg agtttagata tatgaattca 11820

cagggactac tcccacccaa gaatagcata gatgccttca aactcaacat taaattgttg 11880

ggtgttggtg gcaaaccttg tatcaaagta gccactgtac agtctaaaat gtcagatgta 11940

aagtgcacat cagtagtctt actctcagtt ttgcaacaac tcagagtaga atcatcatct 12000

aaattgtggg ctcaatgtgt ccagttacac aatgacattc tcttagctaa agatactact 12060

gaagcctttg aaaaaatggt ttcactactt tctgttttgc tttccatgca gggtgctgta 12120

gacataaaca agctttgtga agaaatgctg gacaacaggg caaccttaca agctatagcc 12180

tcagagttta gttcccttcc atcatatgca gcttttgcta ctgctcaaga agcttatgag 12240

caggctgttg ctaatggtga ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat 12300

gtggctaaat ctgaatttga ccgtgatgca gccatgcaac gtaagttgga aaagatggct 12360

gatcaagcta tgacccaaat gtataaacag gctagatctg aggacaagag ggcaaaagtt 12420

actagtgcta tgcagacaat gcttttcact atgcttagaa agttggataa tgatgcactc 12480

aacaacatta tcaacaatgc aagagatggt tgtgttccct tgaacataat acctcttaca 12540

acagcagcca aactaatggt tgtcatacca gactacaaca catataagaa tacgtgtgat 12600

ggtacaacat ttacttatgc atcagcattg tgggaaatcc aacaggttgt agatgcagat 12660

agtaaaattg ttcagcttag tgaaattagt atggacaatt cacctaattt agcatggcct 12720

cttattgtaa cagctttaag ggccaattct gctgtcaaat tacagaataa tgagcttagt 12780

cctgttgcac taagacaaat gtcttgtgct gccggtacta cacaaactgc ttgcactgat 12840

gacaatgcgt tagcttacta caacacaaca aagggaggta ggtttgtact tgcactgtta 12900

tccgatttac aggatttgaa atgggctaga ttccctaaga gtgatggaac tggtactatc 12960

tatacagaac tggaaccacc ttgtaggttt gttacagaca cacctaaagg tcctaaagtg 13020

aagtatcttt acttcatcaa aggattaaac aacctaaata gaggtatggt acttggtagt 13080

ttagctgcca cagtacgttt acaagctggt aatgcaacag aagttcctgc taattcaact 13140

gtactttctt tctgtgcttt tgctgtagat gctgctaaag cttacaaaga ttatctagct 13200

agtgggggac aaccaatcac taattgtgtt aagatgttgt gtacacacac tggtactggt 13260

caggcaataa cagttacacc ggaagccaat atggatcaag aatcctttgg tggtgcatcg 13320

tgttgtctgt actgccgttg tcatatagat catccaaatc ctaaaggatt ttgtgactta 13380

aaaggtaagt atgtacaaat acctacaact tgtgctaatg accctgtggg ttttacactt 13440

aaaaacacag tctgtaccgt ctgcggtatg tggaaaggtt atggttgtag ttgtgatcaa 13500

ctccgcgaac ccatgcttca gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg 13560

taagtgcagc ccgtcttaca ccgtgcggca caggcactag tactgatgtc gtatatagag 13620

cttttgacat ctacaatgat aaagtagctg gttttgctaa gttcctaaaa actaattgtt 13680

gtcgcttcca agaaaaggac gaagatgaca atctcattga ttcttacttt gtagttaaga 13740

gacacacttt ctctaactac caacatgaag aaacaattta caacctgctt aaggattgtc 13800

cagctgttgc taaacatgac ttctttaagt ttagaataga cggtgacatg gtaccacata 13860

tatcacgtca acgtcttact aaatacacaa tggcagacct cgtctatgct ttaaggcatt 13920

ttgatgaagg taattgtgac acattaaaag aaatacttgt cacatacaat tgttgtgatg 13980

atgactactt caataaaaag gactggtatg attttgtaga aaacccagat atattacgcg 14040

tatacgccaa cttaggtgaa cgtgtacgcc aagctttgtt aaaaacagta cagttctgtg 14100

atgccatgcg aaatgctggt attgttggtg tactgacatt agataatcaa gatctcaatg 14160

gtaactggta tgactttggt gatttcatac aaaccacgcc aggtagtgga gttcctgttg 14220

tagactctta ttattcattg ctcatgccta tattaacctt gaccagggct ttaactgcag 14280

agtcacatgt tgacactgac ttaacaaagc cttacattaa gtgggatttg ttaaaatacg 14340

acttcacgga agagaggtta aaactctttg accgttattt taaatactgg gatcagacat 14400

accacccaaa ttgtgttaac tgtttggatg acagatgcat tctgcattgt gcaaacttta 14460

atgttctgtt ctctacagtg ttcccaccta caagttttgg accactagtg agaaaaatat 14520

ttgttgatgg tgttccattt gtagtttcaa ctggatacca cttcagagag ctaggtgttg 14580

tacataatca ggatgtaaac ttacatagct ctagacttag ttttaaggaa ttacttgtgt 14640

atgctgctga tcctgctatg catgctgctt ctggtaatct attactagat aaacgcacta 14700

cgtgcttttc agtagctgca cttactaaca atgttgcttt tcaaactgtc aaacccggta 14760

attttaacaa ggacttctat gactttgctg tgtctaaggg tttctttaag gaaggaagtt 14820

ctgttgaatt aaaacacttc ttctttgctc aggatggtaa tgctgctatc agcgattatg 14880

actactatcg ttataatcta ccaacaatgt gtgatatcag acaactacta tttgtagttg 14940

aagttgttga taagtacttt gattgttacg atggtggctg tattaatgct aaccaagtca 15000

tcgtcaacaa cctagacaaa tcagctggtt ttccatttaa taaatggggt aaggctagac 15060

tttattatga ttccatgagt tatgaggatc aagatgcact tttcgcatat acaaaacgta 15120

atgtcatccc tactataact caaatgaacc ttaagtatgc cattagtgca aagaatagag 15180

ctcgcaccgt agctggtgtc tctatctgta gtactatgac caatagacag tttcatcaaa 15240

aattactcaa gtcaatagcc gccactagag gagctactgt agtaattgga acaagcaaat 15300

tctatggtgg ttggcacaac atgctcaaaa ctgtttatag tgatgtagaa aaccctcacc 15360

ttatgggttg ggattatcct aaatgtgata gagccatgcc taacatgctt agaattatgg 15420

cctcacttgt tcttgctcgc aaacatacaa cgtgttgtag cttgtcacac cgtttctata 15480

gattagctaa tgagtgtgct caagtattga gtgaaatggt catgtgtggc ggttcactat 15540

atgttaaacc aggtggaacc tcatcaggag atgccacaac tgcttatgct aatagtgtgt 15600

ttaacatttg tcaagctgtc acggccaatg ttaatgcact tttatctact gatggtaaca 15660

aaattgccga taagtatgtc cgcaatttac aacacagact ttatgagtgt ctctatagaa 15720

atagagatgt tgacacagac tttgtgaatg agttttacgc atatttgcgt aaacatttct 15780

caatgatgat actctctgac gatgctgttg tgtgtttcaa tagcacttat gcatctcaag 15840

gtctagtggc tagcataaag aactttaagt cagttcttta ctatcaaaac aacgttttta 15900

tgtctgaagc aaaatgttgg actgagactg accttactaa aggacctcat gaattttgct 15960

ctcaacatac aatgctagtt aaacagggtg atgattatgt gtaccttcct tacccagatc 16020

catcaagaat cctaggtgcc ggttgttttg tagatgatat cgtaaaaaca gatggtacac 16080

ttatgattga acggttcgtg tctttagcta tagatgctta cccacttact aaacatccta 16140

atcaggagta tgctgatgtc tttcatttgt acttacaata catacgtaag ctacatgatg 16200

agttaacagg acacatgtta gacatgtatt ctgttatgct tactaatgat aacacttcaa 16260

ggtattggga acctgagttt tatgaggcta tgtacacacc gcatacagtc ttacaagctg 16320

ttggtgcttg tgttctttgc aattcacaga cttcattaag atgtggtgct tgcatacgta 16380

gaccattctt atgttgtaaa tgctgttacg accatgtcat ctcaacatca cataaattag 16440

tcttgtctgt taatccgtat gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc 16500

aactttactt aggaggtatg agctattact gtaagtcaca taaaccaccc attagttttc 16560

cattgtgtgc taatggacaa gtttttggtc tctacaagaa tacatgtgtt ggtagcgata 16620

atgttactga ctttaatgca attgcaacat gtgactggac aaatgctggt gattacattt 16680

tagctaacac ctgtactgaa agactcaagc tttttgcagc agaaacgctc aaagctactg 16740

aggagacatt taaactgtct tatggtattg ctactgtacg tgaagtgctg tctgacagag 16800

aattacatct ttcatgggaa gttggtaaac ctagaccacc acttaaccga aattatgtct 16860

ttactggtta tcgtgtaact aaaaacagta aagtgcaaat cggagagtac acctttgaaa 16920

aaggtgacta tggtgatgct gttgtttacc gaggtacaac aacttacaaa ctcaacgttg 16980

gtgattattt tgtgctgaca tcacatacag taatgccatt aagtgcacct acactagtgc 17040

cacaagagca ctatgttaga attactggct tatacccaac actcaatatc tcagatgagt 17100

tttctagcaa tgttgcaaat tatcaaaagg ttggtatgca aaagtattct acactccagg 17160

gaccacctgg tactggtaaa agtcattttg ctattggtct agctctctac tacccttctg 17220

ctcgcatagt atatacagct tgctctcatg cagctgttga tgcactatgt gagaaggcat 17280

taaaatattt gcccatagac aaatgtagta gaattatacc tgcacgtgct cgtgtagagt 17340

gttttgataa attcaaggtg aattcaacat tagaacagta tgtcttttgt actgtaaatg 17400

cattgcctga gacgacagca gatatagttg tctttgatga aatttcaatg gccacaaatt 17460

atgatttgag tgttgtcaat gccagattac gtgctaagca ctatgtgtac attggtgatc 17520

ctgctcaatt acctgcacca cgcacattac taactaaggg tacactagaa ccagaatatt 17580

tcaattcagt gtgtagactt atgaaaacta taggtccaga catgttcctc ggaacttgtc 17640

gtagatgtcc tgctgaaatt gttgacactg tgagtgcttt ggtttatgat aataagctta 17700

aggcacataa agacaaatca gctcaatgct ttaaaatgtt ctacaagggt gttatcacgc 17760

atgatgtttc atctgcaatt aacaggccac aaataggcgt ggtaagagaa ttccttacac 17820

gtaaccctgc ttggagaaaa gctgtcttta tttcacctta caattcccag aatgctgtag 17880

cctcaaagat tttgggacta ccaactcaaa ctgttgattc atcacagggc tcagaatatg 17940

actatgtcat attcactcaa accactgaaa cagctcactc ttgtaatgta aacagattca 18000

acgttgctat taccagagca aaagtaggca tactttgcat aatgtctgat agagaccttt 18060

atgacaagtt gcaatttaca agtcttgaaa ttccacgtag gaatgtggca actttacaag 18120

ctgaaaatgt aacaggactc tttaaagatt gtagtaaggt aatcactggg ttacatccta 18180

cacaggcacc tacacactta agtgttgata ctaaattcaa aactgaaggt ttatgtgttg 18240

acatacctgg catacctaag gacatgacct atagaagatt aatctctatg atgggtttca 18300

aaatgaatta ccaggttaat ggttacccta acatgtttat cacccgcgaa gaagctataa 18360

gacatgtacg tgcatggatt ggcttcgatg tcgaaggttg tcatgctact agagaagctg 18420

ttggtaccaa tttaccttta cagctaggtt tttctacagg tgttaaccta gttgctgtac 18480

ctacaggtta tgttgataca cctaataata cagatttttc cagagttagt gctaaaccac 18540

cgcctggaga tcaatttaaa cacctcatac cacttatgta caaaggactt ccttggaatg 18600

tagtgcgtat aaagattgtc caaatgttaa gtgacacact taaaaatctc tctgacagag 18660

tcgtatttgt cttatgggca catggctttg agttgacatc tatgaagtat tttgtgaaga 18720

tcggacctga gcgcacatgt tgtctatgtg atagacgtgc tacatgcttt tccactgctt 18780

cagacactta tgcctgttgg catcattcta ttggatttga ttacgtctat aatccgttta 18840

tgattgatgt tcaacaatgg ggttttacag gtaacctaca aagcaaccat gatctgtatt 18900

gtcaagtcca tggtaatgca catgtagcta gttgtgatgc aatcatgact aggtgtctag 18960

ctgtccacga gtgctttgtt aagcgtgttg actggactat tgaatatcct ataatcggtg 19020

atgaactgaa gattaatgcg gcttgtagaa aggttcaaca catggttgtt aaagctgcat 19080

tattagcaga caaattccca gttcttcacg acattggtaa ccctaaagct attaagtgtg 19140

tacctcaagc tgatgtagaa tggaagttct atgatgcaca gccttgtagt gacaaagctt 19200

acaaaataga agaactgttc tattcttatg ccacacattc tgacaaattc acagatggtg 19260

tatgcctatt ttggaattgc aatgtcgata gatatcctgc taattccatt gtttgtagat 19320

ttgacactag agtgctatct aaccttaact tgcctggttg tgatggtggc agtttgtatg 19380

taaataagca tgcattccac acaccagctt ttgataaaag tgcttttgtt aatctaaagc 19440

aacttccatt tttctattac tctgacagtc catgtgagtc tcatggaaaa caagtagtgt 19500

cagatataga ttatgtacca ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg 19560

gtgctgtctg tagacatcat gctaatgagt acagattgta tctcgatgct tataacatga 19620

tgatctcagc tggctttagc ttgtgggttt acaaacaatt tgatacctat aacctctgga 19680

acacttttac aagacttcag agtttagaaa atgtggcttt taatgttgta aataagggac 19740

actttgatgg acaacagggt gaagtaccag tttctatcat taacaacact gtttacacaa 19800

aagttgatgg tgttgatgta gaattgtttg agaacaaaac cacattacct gttaatgtag 19860

catttgagct ttgggctaag cgcaacatta aaccagtacc agaggtgaaa atactcaata 19920

atttgggtgt ggacattgct gctaatactg tgatctggga ctacaaaaga gatgctccag 19980

cacatatatc tactattggt gtttgttcta tgactgacat agccaagaaa ccaactgaaa 20040

cgatttgtgc accactcact gtcttttttg atggtagagt tgatggtcaa gtagacttat 20100

ttagaaatgc ccgtaatggt gttcttatta cagaaggtag tgttaaaggt ttacaaccat 20160

ctgtaggtcc caaacaagct agtcttaatg gagtcacatt aattggagaa gccgtaaaaa 20220

cacagttcaa ttattacaag aaagtggatg gtgttgtcca acaattacct gaaacttact 20280

ttactcagag tagaaactta caggaattta agcccaggag tcaaatggaa attgatttct 20340

tagaacttgc tatggatgaa ttcattgaac ggtataaatt agaaggctat gccttcgaac 20400

atatcgttta tggagatttt agtcatagtc agttaggtgg tttacatcta ctgattggac 20460

tagctaaacg ttttaaggaa tcaccttttg aacttgaaga ttttattcct atggacagta 20520

cagttaaaaa ctacttcata acagatgcgc aaacaggttc atctaagtgt gtgtgttctg 20580

ttattgatct tttacttgat gacttcgttg aaataataaa gtcccaagat ttatctgtag 20640

tttctaaggt tgtcaaagtg actattgact atacagaaat ctcatttatg ctttggtgta 20700

aagatggcca tgtagaaaca ttttacccaa aattacaatc tagtcaagcg tggcaaccgg 20760

gtgttgctat gcctaatctt tacaaaatgc aaagaatgct attagaaaag tgtgaccttc 20820

aaaattatgg tgatagtgca acattaccta aaggcataat gatgaatgtc gcaaaatata 20880

ctcaactgtg tcaatattta aacacactga cattagctgt accctataat atgagagtta 20940

tccattttgg tgctggttct gataaaggag ttgcaccagg tacagctgtt ttaagacaat 21000

ggttgcctac aggtacgctg cttgtcgatt cagatcttaa tgactttgtc tctgatgcag 21060

attcaacttt gattggtgat tgtgcaactg tacatacagc taataaatgg gatctcatta 21120

ttagtgatat gtacgaccct aagactaaga atgtcacaaa agaaaacgac tctaaagagg 21180

gttttttcac ttacatttgt gggtttatac aacaaaagct agctcttgga ggttccgtgg 21240

ctataaagat aacagaacat tcttggaatg ctgatcttta taagctcatg ggacacttcg 21300

catggtggac agcctttgtt actaatgtga atgcgtcatc atctgaagca tttttaatcg 21360

gatgtaacta ccttggcaaa ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt 21420

acatattttg gaggaataca aatccaattc agctttcttc ttattcttta ttcgacatga 21480

gtaaattccc ccttaaatta aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca 21540

atgatatgat tctctctctt cttagtaaag gtagacttat aattagagaa aacaacagag 21600

ttgttatttc tagtgatgtt cttgttaaca actaaacgaa caatgtttgt ttttcttgtt 21660

ttattgccac tagtctctag tcagtgtgtt aatcttacaa ccagaactca attaccccct 21720

gcatacacta attctttcac acgtggtgtt tattaccctg acaaagtttt cagatcctca 21780

gttttacatt caactcagga cttgttctta cctttctttt ccaatgttac ttggttccat 21840

gctatacatg tctctgggac caatggtact aagaggtttg ataaccctgt cctaccattt 21900

aatgatggtg tttactttgc ttccactgag aagtctaaca taataagagg ctggattttt 21960

ggtactactt tagattcgaa aacccagtcc ctacttattg ttaataacgc tactaatgtt 22020

gttatcaaag tctgtgaatt tcaattttgt aacgatccat ttttgggtgt ttattaccac 22080

aaaaacaaca aaagttggat ggaaagtgag ttcagagttt attctagtgc gaataattgc 22140

acttttgaat acgtctctca gccttttctt atggaccttg aaggaaaaca gggtaatttc 22200

aaaaatctta gggaatttgt gttcaagaat attgatggtt acttcaagat atactctaag 22260

cacacgccta ttaatttagt gcgtgatctc cctcagggtt tttcggcttt agaaccattg 22320

gtagatttgc caataggtat taacatcact aggtttcaaa ctttacttgc tttacataga 22380

agttatttaa ctcctggtga ttcttcttca ggttggacag ctggtgctgc agcttattat 22440

gtgggttatc ttcaacctag gacttttcta ctgaagtaca atgaaaatgg aaccattaca 22500

gatgctgtag actgtgcact tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc 22560

actgtagaaa aaggaatcta tcaaacttct aactttagag tccaaccaac agaatctatt 22620

gttagatttc ctaacatcac aaacttgtgc ccttttggtg aagtttttaa cgccaccaga 22680

tttgcatctg tttatgcttg gaacaggaag agaatcagca actgtgttgc tgattattct 22740

gtcctgtata attccgcatc attttccact tttaagtgtt atggagtgtc tcctactaaa 22800

ttaaatgatc tctgctttac taatgtctat gcagattcat ttgtaattag aggtgatgaa 22860

gtcagacaaa tcgctccagg gcaaactgga aagattgctg attataacta caaattacca 22920

gatgatttta caggctgcgt tatagcttgg aattctaaca atcttgattc taaggttggt 22980

ggtaattata attacctgta cagattgttt aggaagtcta atctcaaacc ttttgagaga 23040

gatatttcaa ctgaaatcta tcaggccggt agcacacctt gtaatggtgt tgaaggtttt 23100

aattgttact ttcctctgca atcatatggt ttccaaccca ctaatggtgt tggttaccaa 23160

ccatacagag tagtagtact ttcttttgaa cttctacatg caccagcaac tgtttgtgga 23220

cctaaaaagt ctactaattt ggttaagaac aagtgtgtca atttcaactt caatggttta 23280

acaggcacag gtgttcttac tgagtctaac aaaaagtttc tgcctttcca acaatttggc 23340

agagacattg ctgacactac tgatgctgtt cgtgatccac aaacacttga gattcttgac 23400

attacaccat gttcttttgg tggtgtcagt gttataacac caggaacaaa tacttctaac 23460

caggttgctg ttctttatca ggatgttaac tgcacagaag tccctgttgc tattcatgca 23520

gatcaactta ctcctacttg gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt 23580

gcaggctgtt taataggggc tgaacatgtc aacaactcat atgagtgtga catacccatt 23640

ggtgcaggta tatgcgctag ttatcagact cagactaatt ctcctcggag agcaagaagt 23700

gtagctagtc aatccatcat tgcctacact atgtcacttg gtgcagaaaa ttcagttgct 23760

tactctaata actctattgc catacccaca aattttacta ttagcgttac cacagaaatt 23820

ctaccagtgt ctatgaccaa gacatcagta gattgtacaa tgtacatttg tggtgattca 23880

actgaatgca gcaatctttt gttgcaatat ggcagttttt gtacacaatt aaaccgtgct 23940

ttaactggaa tagctgttga acaagacaaa aacacccaag aagtttttgc acaagtcaaa 24000

caaatttaca agacaccacc aattaaagat tttggcggtt ttaattttag ccagatactg 24060

ccagatccat caaaaccaag caagaggtca tttattgaag atctactgtt caacaaagtg 24120

acacttgcag atgctggctt catcaaacaa tatggtgatt gccttggtga tattgctgct 24180

agagacctca tttgtgcaca aaagtttaac ggccttactg ttttgccacc tttgctcaca 24240

gatgaaatga ttgctcaata cacttctgca ctgttagcag gtacaatcac ttctggttgg 24300

acttttggtg caggtgctgc attacaaata ccatttgcta tgcaaatggc ttataggttt 24360

aatggtattg gagttacaca gaatgttctc tatgagaacc aaaaattgat tgccaaccaa 24420

tttaatagtg ctattggcaa aattcaagac tcactttctt ccacagcaag tgcacttgga 24480

aaacttcaag atgtggtcaa ccaaaatgca caagctttaa acacgcttgt taaacaactt 24540

agctccaatt ttggtgcaat ttcaagtgtt ttaaacgaca tcctttcacg tcttgacaaa 24600

gttgaggctg aagtgcaaat tgataggttg atcacaggca gacttcaaag tttgcagaca 24660

tatgtgactc aacaattaat tagagctgca gaaatcagag cttctgctaa tcttgctgct 24720

actaaaatgt cagagtgtgt acttggacaa tcaaaaagag ttgacttttg cggaaagggc 24780

tatcatctta tgtcatttcc tcagtcagca cctcatggtg tcgtcttttt gcatgtgact 24840

tatgtccctg cacaagaaaa gaacttcaca actgctcctg ccatttgtca tgatggaaaa 24900

gcacactttc ctcgtgaagg tgtctttgtt tcaaatggca cacactggtt tgtaacacaa 24960

aggaattttt atgaaccaca aatcattact acagacaaca catttgtgtc tggtaactgt 25020

gatgttgtaa taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac 25080

tcattcaagg aggagcttga taaatacttc aagaaccata cctcaccaga tgttgattta 25140

ggtgacatct ctggcattaa tgcttcagtt gtaaacattc agaaagaaat cgaccgcctc 25200

aatgaggttg ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat 25260

gagcagtata taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc 25320

atagtaatgg tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc 25380

tgttgttctt gtggatcctg ctgcaaattt gacgaggacg actctgagcc agtgctcaaa 25440

ggagtcaaat tacattacac ataaacgaac ttatggattt gtttatgaga atcttcacaa 25500

ttggaactgt aactttgaag caaggtgaaa tcaaggatgc tactccttca gattttgtta 25560

gagctactgc aacgataccg atacaagcat cacttccttt cggatggctt attgttggcg 25620

ttgcacttct tgctgttttt cagagcgctt ccaaaatcat aaccctcaaa aagagatggc 25680

aactagcact ctccaagggt gttcactttg tttgcaactt gctgttgttg tttgtaacag 25740

tttactcaca tcttttgctt gttgctgctg gccttgaagc cccttttctc tatctttatg 25800

ctttagtcta cttcttgcag agtataaact ttgtacgcat aataatgagg ctttggcttt 25860

gctggaaatg ccgttccaaa aacccattac tttatgatgc caactatttt ctttgctggc 25920

atactaattg ttacgactat tgtatacctt acaatagtgt aacttcttca attgtcatta 25980

cttcaggtga tggcacaaca agtcctattt ctgaacatga ctaccagatt ggtggttata 26040

ctgaaaaatg ggaatctgga gtaaaagact gtgttgtatt acacagttac ttcacttcag 26100

actattacca gctgtactca actcaattga gtacagacac tggtgttgaa catgttacct 26160

tcttcatcta caataaaatc gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg 26220

acgtttcatc cggagttgtt aatccagtaa tggaaccaat ttatgatgaa ccgacgacga 26280

ctactagcgt gcctttgtaa gcacaagctg atgagtacga acttatggca gattccaacg 26340

gtactattac cgttgaggag ctgaaaaagc tccttgaaca atggaaccta gtaataggtt 26400

tcctattcct tacatggatt tgcctgctgc aatttgccta tgccaacagg aataggtttt 26460

tgtacatcat taagttgatt ttcctctggc tgttatggcc agtaacttta gcttgttttg 26520

tgcttgctgc tgtttacaga ataaattgga tcaccggtgg aattgctatt gcaatggctt 26580

gtcttgtagg attgatgtgg ctaagctact tcattgcttc tttcagactg tttgcgcgta 26640

cgcgttccat gtggtcattc aatccagaaa ctaacattct tctcaacgtg ccactccatg 26700

gaactattct gactagaccg cttctagaaa gtgaactcgt aatcggagct gttatccttc 26760

gtggacatct tcgtattgct ggacatcatc taggacgctg tgacatcaag gatctaccta 26820

aagaaatcac tgttgctaca tcacgaacgc tttcttatta caaattggga gcttcacagc 26880

gtgtagcagg tgattcaggt tttgctgcat atagtcgcta caggattggc aactataaat 26940

taaacacaga ccattccagt agcagtgaca atattgcttt gcttgtacag taaacgaaca 27000

tgaaaattat tcttttcttg gcactgataa cactcgctac ttgtgagctt tatcactacc 27060

aagagtgtgt tagaggtaca acagtacttt taaaagaacc ttgctcgtcg ggaacatacg 27120

agggcaattc accatttcat cctctagctg ataacaaatt tgcactgact tgctttagca 27180

ctcaatttgc ttttgcttgt cctgacggcg taaaacacgt ctatcagtta cgtgccagat 27240

cagtttcacc taaactgttc atcagacaag aggaagttca agaactttac tctccaattt 27300

ttcttattgt tgcggcaata gtgtttataa cactttgctt cacactcaaa agaaagacag 27360

aatgattgaa ctttcattaa ttgacttcta tttgtgcttt ttagcctttc tgctattcct 27420

tgttttaatt atgcttatta tcttttggtt ctcacttgaa ctgcaagatc ataatgaaac 27480

ttgtcacgcc taaacgaaca tgaaatttct tgttttctta ggaatcatca caactgtagc 27540

tgcatttcac caagaatgta gtttacagtc atgtactcaa catcaaccat atgtagttga 27600

tgacccgtgt cctattcact tctattctaa atggtatatc agagtaggag ctagaaaatc 27660

agcaccttta attgaattgt gcgtggatga ggctggttct aaatcaccca ttcagtacat 27720

cgatatcggt aattatacag tttcctgttt accttttaca attaactgcc aggaacctaa 27780

attgggtagt cttgtagtgc gttgttcgtt ctacgaggac tttttagagt atcatgacgt 27840

tcgtgttgtt ttagatttca tctaaacgaa caaactaaaa tgtctgataa tggacctcaa 27900

aatcagcgaa atgcacctcg cattacgttt ggtggaccat cagattcaac tggcagtaac 27960

cagaatggag aacgaagtgg tgcgcgatca aaacaacgcc gcccgcaagg tttacccaat 28020

aatactgcgt cttggttcac cgctctcact caacatggca aggaagattt aaaattccct 28080

cgaggacaag gcgttccaat taacaccaat agcagtccag atgaccaaat tggctactac 28140

cgccgcgcca caagacgaat tcgtggtggt gatggtaaaa tgaaagatct cagtccaaga 28200

tggtatttct actatctagg aactgggcca gaagctggac ttccttatgg tgctaacaaa 28260

gatggcatca tatgggttgc aactgaggga gccttgaata caccaaaaga tcacattggc 28320

accagaaatc ctgctaacaa tgctgcaatc gtgctacaac ttcctcaagg aacaacatta 28380

ccaaaaggtt tttacgcaga agggtctaga ggtggaagtc aagcctcttc tagatcatca 28440

tcacgtagtc gcaacagttc aagaaattca actccaggtt caagtagagg aacttctcct 28500

gctagaatgg ctggaaatgg aggtgatgct gctcttgctt tgttactact tgacagattg 28560

aaccagcttg agagcaaaat gtctggtaaa ggccaacaac aacaaggcca aactgtcact 28620

aagaaatctg ctgctgaggc ttctaagaag cctagacaaa aacgtactgc cactaaagca 28680

tacaatgtaa cacaagcttt cggcagacgt ggtccagaac aaactcaagg aaattttggg 28740

gatcaggaac taatcagaca aggaactgat tacaaacatt ggccgcaaat tgcacaattt 28800

gctccttctg cttcagcgtt ctttggaatg tcgagaattg gaatggaagt cacaccttcg 28860

ggaacatggt tgacctatac aggtgccatc aaattggatg acaaagatcc aaatttcaaa 28920

gatcaagtca ttttgctgaa taagcatatt gacgcataca aaacattccc accaacagag 28980

cctaaaaagg acaaaaagaa gaaggctgat gaaactcaag ccttaccgca gagacagaag 29040

aaacagcaaa ctgtgactct tcttcctgct gcagatttgg atgatttctc caaacaattg 29100

caacaatcca tgagcagtgc tgactcaact caggcctaaa ctcatgcaga ccacacaagg 29160

cagatgggct atataaacgt tttcgctttt ccgtttacga tatatagtct actcttgtgc 29220

agaatgaatt ctcgtaacta catagcacaa gtagatgtag ttaactttaa tctcacatag 29280

caatctttaa tcagtgtgta acattaggga ggacttgaaa gagccaccac attttcaccg 29340

aggccacgcg gagtacgatc gagtgtacag tgaacaatgc tagggagagc tgcctatatg 29400

gaagagccct aatgtgtaaa attaatttta gtagtgctat ccccatgtga ttttaatagc 29460

ttcttaggag aatgacaaaa aaaaacaaaa aaaa 29494

<210> 42

<211> 29348

<212> DNA

<213> Artificial sequence

<220>

<223> Synthesis of optimized sequence E-protein and ORF8 double deletion

<400> 42

caaatataac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 60

taatacgact cactataggg attaaaggtt tataccttcc caggtaacaa accaaccaac 120

tttcgatctc ttgtagatct gttctctaaa cgaactttaa aatctgtgtg gctgtcactc 180

ggctgcatgc ttagtgcact cacgcagtat aattaataac taattactgt cgttgacagg 240

acacgagtaa ctcgtctatc ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga 300

tcatcagcac atctaggttt cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc 360

cctggtttca acgagaaaac acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac 420

gtgttagtac gtggttttgg agattcagtg gaagaagtct tatcagaggc acgtcaacat 480

cttaaagatg gcacttgtgg cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa 540

cagccctatg tgttcatcaa acgttctgat gctagaactg cacctcatgg tcatgttatg 600

gttgagctgg tagcagaatt agaaggtatt cagtacggtc gtagtggtga gacattaggt 660

gttttagttc ctcatgtggg cgaaatacca gtggcttacc gcaaagttct tcttagaaag 720

aacggtaata aaggagctgg tggccatagt tacggcgctg atttaaagtc atttgactta 780

ggcgacgagc ttggcactga tccttatgaa gatttccaag aaaactggaa cactaaacat 840

agcagtggtg ttacccgtga actcatgcgt gagttaaatg gaggtgcata cactcgctat 900

gtcgataaca acttctgtgg acctgatggt taccctcttg agtgcattaa agaccttcta 960

gcacgtgctg gtaaagcttc atgcactttg tccgaacaac tggactttat tgacactaag 1020

aggggtgtat actgctgccg tgaacatgag catgaaattg cttggtacac ggaacgttct 1080

gaaaagagct atgaattgca gacacctttt gaaattaaac tggcaaagaa atttgacacc 1140

ttcaatgggg aatgtccaaa ttttgtattt cccctcaatt ccataatcaa gactattcaa 1200

ccaagggttg aaaagaaaaa gcttgatggc tttatgggta gaattcgatc tgtctatcca 1260

gttgcgtcac caaatgaatg caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat 1320

tgtggtgaaa cttcatggca gacgggcgat tttgttaaag ccacttgcga attttgtggc 1380

actgagaatt tgactaaaga aggtgccact acttgtggtt acttacccca aaatgctgtt 1440

gttaaaattt actgtccagc atgtcacaat tcagaagtag gacctgagca tagtcttgcc 1500

gaataccata atgaatctgg cttgaaaacc attcttcgta agggtggtcg cactattgct 1560

tttggaggat gtgtgttctc ttatgttggt tgccataaca agtgtgctta ttgggttcca 1620

cgtgcttcag ctaacatagg ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt 1680

cttaatgaca accttcttga aatactccaa aaagagaaag tcaacatcaa tattgttggt 1740

gactttaaac ttaatgaaga gatcgccatt attttggcat ctttttctgc ttccacaagt 1800

gcttttgtgg aaactgtgaa aggtttggat tataaagcat tcaaacagat tgttgaatcc 1860

tgtggtaatt ttaaggttac aaagggaaaa gctaaaaaag gtgcctggaa tattggtgaa 1920

cagaaatcaa tactgagtcc tctttatgca tttgcatcag aggctgctcg tgttgtacga 1980

tcaattttct cccgcactct tgaaactgct caaaattctg tgcgtgtttt acagaaggcc 2040

gctataacaa tactagatgg aatttcacag tattcactga gactcattga tgctatgatg 2100

ttcacatctg atttggctac taacaatcta gttgtaatgg cctacattac aggtggtgtt 2160

gttcagttga cttcgcagtg gctaactaac atctttggca ctgtttatga aaaactcaaa 2220

cccgtccttg attggcttga agagaagttt aaggaaggtg tagagtttct tagagacggt 2280

tgggagattg ttaaattcat ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc 2340

acctgtgcta aggaaattaa ggagagtgtt cagacattct ttaagcttgt aaacaagttt 2400

ttggctttgt gtgctgactc tatcattatt ggtggagcta aacttaaagc cttgaattta 2460

ggtgaaacat ttgtcacgca ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa 2520

gaaactggcc tactcatgcc tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa 2580

acacttccca cagaagtgtt aacagaggaa gttgtcttga aaactggtga tttacaacca 2640

ttagaacaac ctactagtga agctgttgaa gctccattgg ttggtacacc agtttgtatt 2700

aacgggctta tgttgctcga aatcaaagac acagaaaagt actgtgccct tgcacctaat 2760

atgatggtaa caaacaatac cttcacactc aaaggcggtg caccaacaaa ggttactttt 2820

ggtgatgaca ctgtgataga agtgcaaggt tacaagagtg tgaatatcac ttttgaactt 2880

gatgaaagga ttgataaagt acttaatgag aagtgctctg cctatacagt tgaactcggt 2940

acagaagtaa atgagttcgc ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca 3000

gtatctgaat tacttacacc actgggcatt gatttagatg agtggagtat ggctacatac 3060

tacttatttg atgagtctgg tgagtttaaa ttggcttcac atatgtattg ttctttctac 3120

cctccagatg aggatgaaga agaaggtgat tgtgaagaag aagagtttga gccatcaact 3180

caatatgagt atggtactga agatgattac caaggtaaac ctttggaatt tggtgccact 3240

tctgctgctt tacaacctga agaagaacaa gaagaagatt ggttagatga tgatagtcaa 3300

caaactgttg gtcaacaaga cggcagtgag gacaatcaga caactactat tcaaacaatt 3360

gttgaggttc aacctcaatt agagatggaa cttacaccag ttgttcagac tattgaagtg 3420

aatagtttta gtggttatct taaacttact gacaatgtat acatcaagaa tgcagacatt 3480

gtggaagaag ctaaaaaggt aaaaccaaca gtggttgtta atgcagccaa tgtttacctt 3540

aaacatggag gaggtgttgc aggagcctta aataaggcta ctaacaatgc catgcaagtt 3600

gaatctgatg attacatagc tactaatgga ccacttaaag tgggtggtag ttgtgtttta 3660

agcggacaca atcttgctaa acactgttta catgttgtcg gcccaaatgt taacaaaggt 3720

gaagatattc aacttcttaa gagtgcttat gaaaatttta accagcacga agttctactt 3780

gcaccattat tatcagctgg tatttttggt gctgacccta tacattcttt aagagtttgt 3840

gtagatactg ttcgcacaaa tgtctactta gctgtctttg ataaaaatct ctatgacaaa 3900

cttgtttcaa gctttttgga aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag 3960

attcctaaag aggaagttaa gccatttata actgaaagta aaccttcagt tgaacagaga 4020

aaacaagatg ataagaagat caaagcttgt gttgaagaag ttacaacaac tctggaagaa 4080

actaagttcc tcacagaaaa cttgctcctt tatatcgaca ttaatggcaa tcttcatcca 4140

gattctgcca ctcttgttag tgacattgac atcactttct taaagaaaga tgctccatat 4200

atagtgggtg atgttgttca agagggtgtt ttaactgctg tggttatacc tactaaaaag 4260

gctggtggca ctactgaaat gctagcgaaa gctttgagaa aagtgccaac agacaattat 4320

ataaccactt acccgggtca gggtttaaat ggttacactg tagaggaggc aaagacagtg 4380

cttaaaaagt gtaaaagtgc cttttacatt ctaccatcta ttatctctaa tgagaagcaa 4440

gaaattcttg gaactgtttc ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca 4500

cgcaaattaa tgcctgtctg tgtggaaact aaagccatag tttcaactat acagcgtaaa 4560

tataagggta tcaagataca agagggtgtg gttgattatg gtgctagatt ttacttttac 4620

accagtaaaa caactgtagc gtcacttatc aacacactta acgatctaaa tgaaactctt 4680

gttacaatgc cacttggcta tgtaacacat ggcttaaatt tggaagaagc tgctcggtat 4740

atgagatctc tcaaagtgcc agctacagtt tctgtttctt cacctgatgc tgttacagcg 4800

tataatggtt atcttacttc ttcttctaaa acacctgaag aacattttat tgaaaccatc 4860

tcacttgctg gttcctataa agattggtcc tattctggac aatctacaca actaggtata 4920

gaatttctta agagaggtga taaaagtgta tattacacgt ccaatcctac cacattccac 4980

ctagatggtg aagttatcac ctttgacaat cttaagacac ttctttcttt gagagaagtg 5040

aggactatta aggtgtttac aacagtagac aacattaacc tccacacgca agttgtggac 5100

atgtcaatga catatggaca acagtttggt ccaacttatt tggatggagc tgatgttact 5160

aagataaaac ctcataactc acatgaaggt aaaacatttt acgttttgcc taatgatgac 5220

actctacgtg ttgaggcttt tgagtactac cacacaactg atcctagttt tctgggtagg 5280

tacatgtcag cattaaatca cactaaaaag tggaaatacc cacaagttaa tggtttaact 5340

tcgattaaat gggcagataa caactgttat cttgccactg cattgttaac actccaacaa 5400

atagagttga agtttaatcc acctgctcta caagatgctt attacagagc aagggctggt 5460

gaagctgcta acttttgtgc acttatctta gcctactgta ataagacagt aggtgagtta 5520

ggtgatgtta gagaaacaat gagttacttg tttcaacatg ccaatttaga ttcttgcaaa 5580

agagtcttga acgtggtgtg taaaacttgt ggacaacagc agacaaccct taagggtgta 5640

gaagctgtta tgtacatggg cacactttct tatgaacaat tcaagaaagg tgttcagata 5700

ccttgtacgt gtggtaaaca agctacaaaa tatctagtac aacaggagtc accttttgtt 5760

atgatgtcag caccacctgc tcagtatgaa cttaagcatg gtacatttac ttgtgctagt 5820

gagtacactg gtaattacca gtgtggtcac tataagcata taacttctaa ggaaactttg 5880

tattgcatag acggtgcttt acttacaaag tcctcagaat acaaaggtcc tattacggat 5940

gttttctaca aagaaaacag ttacacaaca accataaaac cagttactta taagttggat 6000

ggtgttgttt gtacagaaat tgaccctaag ttggacaatt attataagaa ggacaactct 6060

tatttcacag agcaaccaat tgatcttgta ccaaaccaac catatccaaa cgcaagcttc 6120

gataatttta agttcgtatg cgataatatc aaatttgctg atgatctcaa ccagttaact 6180

ggttataaga aacctgcttc aagagagctt aaagttacat ttttccctga cttaaatggt 6240

gatgtggtgg ctattgatta taaacactac acaccctctt ttaagaaagg agctaaattg 6300

ttacataagc ctattgtttg gcatgttaac aatgcaacta ataaagccac gtataaacca 6360

aatacctggt gtatacgttg tctttggagc acaaaaccag ttgaaacatc aaattcgttt 6420

gatgtactga agtcagagga cgcgcaggga atggataatc ttgcatgtga agatctaaaa 6480

ccagtctctg aagaagtagt ggaaaatcct accatacaga aagacgttct tgagtgtaat 6540

gtgaaaacta ccgaagttgt aggagacatt atacttaaac cagcaaataa tagtttgaag 6600

atcacagaag aggttggcca cacagatcta atggctgctt atgtagacaa ttctagtctt 6660

actattaaga aacctaatga actctctaga gtattaggtt tgaaaaccct tgctactcat 6720

ggtttagctg ctgttaatag tgtcccttgg gatactatag ctaattatgc taagcctttt 6780

cttaacaaag ttgttagtac aactactaac atagttacac ggtgtcttaa tcgtgtttgt 6840

actaattata tgccttactt ctttacttta ttgctacaat tgtgtacttt tactagaagt 6900

acaaattcta gaatcaaggc atctatgccg actactatag caaagaatac tgttaagagt 6960

gtcggtaaat tttgtctaga ggcttcattt aattatctca agtcacctaa cttttctaag 7020

ctgataaaca ttatcatctg gtttttgcta ttaagtgttt gcctaggttc tttaatctac 7080

tcaaccgctg ctttaggtgt tttaatgtct aatttaggca tgccttctta ctgtactggt 7140

tacagagaag gctatttgaa ctctactaat gtcactattg caacctactg tactggatct 7200

ataccttgta gtgtttgtct tagtggttta gattctttag acacctatcc ttctcttgaa 7260

actatacaga ttaccatttc atctttcaaa tgggatttaa ctgcttttgg cttagttgca 7320

gagtggtttt tggcatatat tcttttcact aggtttttct atgtacttgg attggctgca 7380

atcatgcaat tgtttttcag ctattttgca gtccatttta ttagtaactc ttggcttatg 7440

tggcttataa ttaatcttgt gcagatggcc ccgatttcag ctatggttag aatgtacatc 7500

ttctttgcct cattttatta tgtgtggaaa agttatgtgc atgttgtaga cggttgtaat 7560

tcatcaactt gtatgatgtg ttacaaacgt aatagagcaa caagagtcga atgtacaact 7620

attgttaatg gtgttagaag gtccttttat gtctatgcta atggaggtaa aggcttttgc 7680

aaactacaca attggaattg tgttaattgt gatacattct gtgctggtag tacatttatt 7740

agtgatgaag ttgcgagaga cttgtcacta cagtttaaaa gaccaataaa tcctactgac 7800

caatcttctt acatcgttga tagtgttaca gtgaagaatg gttccatcca tctttacttt 7860

gataaagctg gtcaaaagac ttatgaaaga cattctctct ctcattttgt taacttagac 7920

aacctgagag ctaataacac taaaggttca ttgcctatta atgttatcgt tttcgacggt 7980

aaatcaaaat gtgaagaatc atctgcaaaa tcagcgtctg tttactacag tcagcttatg 8040

tgtcaaccta tactgttact agatcaggca ttagtgtctg atgttggtga tagtgcggaa 8100

gttgcagtta aaatgtttga tgcttacgtt aatacgtttt catcaacttt taacgtacca 8160

atggaaaaac tcaaaacact agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc 8220

ttagacaatg tcttatctac gtttatttca gcagctcggc aagggtttgt tgattcagat 8280

gtagaaacta aagatgttgt tgaatgtctt aaattgtcac atcaatctga catagaagtt 8340

actggcgata gttgtaataa ctatatgctc acctataaca aagttgaaaa catgacaccc 8400

cgtgaccttg gtgcttgtat tgactgtagt gctagacata ttaatgcgca ggtagcaaaa 8460

agtcacaaca ttgctttgat atggaacgtt aaagatttca tgtcattgtc tgaacaacta 8520

cgaaaacaaa tacgtagtgc tgctaaaaag aataacttac ccttcaagtt gacatgtgca 8580

actactagac aagttgttaa tgttgtaaca acaaagatag cacttaaggg tggtaaaatt 8640

gtgaataact ggttgaagca gcttattaaa gttacacttg tgttcctttt tgttgctgct 8700

attttctatc tgataacacc tgttcatgtc atgtctaaac atactgactt ttcaagtgaa 8760

atcataggat acaaggctat tgatggtggt gtcactcgtg acatagcatc tacagatact 8820

tgttttgcta acaaacatgc tgattttgac acatggttta gccagcgtgg tggtagttat 8880

actaatgaca aagcttgccc attgattgct gcagtcataa caagagaagt gggttttgtc 8940

gttcctggtt tgcctggaac gatattacgc acaactaatg gtgacttttt gcatttctta 9000

cctagagttt ttagtgcagt tggtaacatc tgttacacac catcaaaact tatagagtac 9060

actgactttg caacatcagc ttgtgttttg gctgctgaat gtacaatttt taaagacgct 9120

tctggtaagc cagtaccata ttgttatgat accaatgtac tagaaggttc tgttgcttat 9180

gaaagtttac gccctgacac acgttatgtg ctcatggatg gctctattat tcaatttcct 9240

aacacctacc ttgaaggttc tgtaagagtg gtaacaactt ttgattctga gtactgtagg 9300

cacggcactt gtgaaagatc agaagctggt gtttgtgtat ctactagtgg tagatgggta 9360

cttaacaacg attattacag atctttacca ggagttttct gtggtgtaga tgctgtaaat 9420

ttgcttacta acatgtttac accactaatt caacctattg gtgctttgga catatcagca 9480

tctatagtag ctggtggtat tgtagctatc gtagtaacat gccttgccta ctattttatg 9540

aggtttagac gtgcttttgg tgaatacagt catgtagttg cctttaatac tctcctattc 9600

cttatgtcat tcactgtact ctgtttaaca ccagtttact cattcttacc tggtgtttat 9660

tctgttattt acctgtactt gacattttat ctgactaatg atgtttcttt tctcgcacat 9720

attcagtgga tggttatgtt cacaccttta gtacctttct ggataacaat tgcttacatc 9780

atttgtattt ccacaaagca tttctattgg ttctttagta attacctaaa gagacgtgta 9840

gtctttaatg gtgtttcctt tagtactttt gaagaagctg cgctgtgcac ctttttgtta 9900

aataaggaga tgtatctaaa gttgcgtagt gatgtgctat tacctcttac gcaatataat 9960

agatacttag ctctttataa caagtacaag tatttcagtg gagcaatgga tacaactagc 10020

tacagagaag ctgcttgttg tcatctcgca aaggctctca atgacttcag taactcaggt 10080

tctgatgttc tttaccaacc accacaaacc tctatcacct cagctgtttt gcagagtggt 10140

tttagaaaaa tggcattccc atctggtaaa gttgagggtt gtatggtaca agtaacttgt 10200

ggtacaacta cacttaacgg tctttggctt gatgacgtag tttactgtcc aagacatgtg 10260

atctgcacct ctgaagatat gcttaaccct aattatgaag atctactcat ccgtaagtct 10320

aatcataact tcttggtaca ggctggtaat gttcaactca gggttattgg acattctatg 10380

caaaattgtg tacttaagct taaggttgat acagccaatc ctaagacacc taagtataag 10440

tttgttcgca ttcaaccagg acagactttt tcagtgttag cttgttacaa tggttcacca 10500

tctggtgttt accaatgtgc tatgaggccc aatttcacta ttaagggttc attccttaat 10560

ggttcatgtg gtagtgttgg ttttaacata gattatgact gtgtctcttt ttgttacatg 10620

caccatatgg aattaccaac tggagttcat gctggcacag acttagaagg taacttttat 10680

ggaccttttg ttgacaggca aacagcacaa gcagctggta cagatacaac tattacagtt 10740

aatgttcttg cttggttgta cgctgctgtt ataaatggag acaggtggtt tctcaatcga 10800

tttaccacaa ctcttaatga ctttaacctt gtggctatga agtacaatta tgaacctcta 10860

acacaagacc atgttgacat actaggacct ctttctgctc aaactggaat tgccgtttta 10920

gatatgtgtg cttcattaaa agaacttctg caaaatggta tgaatggacg taccatattg 10980

ggtagtgctt tattagaaga tgagtttaca ccttttgatg ttgttagaca atgctcaggt 11040

gttactttcc aaagtgcagt gaaaagaaca atcaagggta cacaccactg gttgttactc 11100

acaattttga cttcactttt agttttagtc cagagtactc aatggtcttt gttctttttc 11160

ttctacgaaa atgccttttt accttttgct atgggtatta ttgctatgtc tgcttttgca 11220

atgatgtttg tcaaacataa gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc 11280

actgtagctt actttaatat ggtctacatg cctgctagtt gggtgatgcg tattatgaca 11340

tggttggata tggttgatac tagtttgtct ggttttaagc taaaagactg tgttatgtat 11400

gcatcagctg tagtgttact aatccttatg acagcaagaa ctgtgtatga tgatggtgct 11460

aggagagtgt ggacacttat gaatgtcttg acactcgttt ataaagttta ctatggcaac 11520

gctttagatc aagccatttc catgtgggct cttataatct ctgttacttc taactactca 11580

ggtgtagtta caactgtcat gtttttggcc agaggtattg tttttatgtg tgttgagtat 11640

tgccctattt tcttcataac tggtaataca cttcagtgta taatgctagt ctattgtttc 11700

ttaggctatt tttgtacttg ttacttcggc ctcttttgtt tactcaaccg ctactttaga 11760

ctgactcttg gtgtttatga ttacttagtg tctacacagg agtttagata tatgaattca 11820

cagggactac tcccacccaa gaatagcata gatgccttca aactcaacat taaattgttg 11880

ggtgttggtg gcaaaccttg tatcaaagta gccactgtac agtctaaaat gtcagatgta 11940

aagtgcacat cagtagtctt actctcagtt ttgcaacaac tcagagtaga atcatcatct 12000

aaattgtggg ctcaatgtgt ccagttacac aatgacattc tcttagctaa agatactact 12060

gaagcctttg aaaaaatggt ttcactactt tctgttttgc tttccatgca gggtgctgta 12120

gacataaaca agctttgtga agaaatgctg gacaacaggg caaccttaca agctatagcc 12180

tcagagttta gttcccttcc atcatatgca gcttttgcta ctgctcaaga agcttatgag 12240

caggctgttg ctaatggtga ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat 12300

gtggctaaat ctgaatttga ccgtgatgca gccatgcaac gtaagttgga aaagatggct 12360

gatcaagcta tgacccaaat gtataaacag gctagatctg aggacaagag ggcaaaagtt 12420

actagtgcta tgcagacaat gcttttcact atgcttagaa agttggataa tgatgcactc 12480

aacaacatta tcaacaatgc aagagatggt tgtgttccct tgaacataat acctcttaca 12540

acagcagcca aactaatggt tgtcatacca gactacaaca catataagaa tacgtgtgat 12600

ggtacaacat ttacttatgc atcagcattg tgggaaatcc aacaggttgt agatgcagat 12660

agtaaaattg ttcagcttag tgaaattagt atggacaatt cacctaattt agcatggcct 12720

cttattgtaa cagctttaag ggccaattct gctgtcaaat tacagaataa tgagcttagt 12780

cctgttgcac taagacaaat gtcttgtgct gccggtacta cacaaactgc ttgcactgat 12840

gacaatgcgt tagcttacta caacacaaca aagggaggta ggtttgtact tgcactgtta 12900

tccgatttac aggatttgaa atgggctaga ttccctaaga gtgatggaac tggtactatc 12960

tatacagaac tggaaccacc ttgtaggttt gttacagaca cacctaaagg tcctaaagtg 13020

aagtatcttt acttcatcaa aggattaaac aacctaaata gaggtatggt acttggtagt 13080

ttagctgcca cagtacgttt acaagctggt aatgcaacag aagttcctgc taattcaact 13140

gtactttctt tctgtgcttt tgctgtagat gctgctaaag cttacaaaga ttatctagct 13200

agtgggggac aaccaatcac taattgtgtt aagatgttgt gtacacacac tggtactggt 13260

caggcaataa cagttacacc ggaagccaat atggatcaag aatcctttgg tggtgcatcg 13320

tgttgtctgt actgccgttg tcatatagat catccaaatc ctaaaggatt ttgtgactta 13380

aaaggtaagt atgtacaaat acctacaact tgtgctaatg accctgtggg ttttacactt 13440

aaaaacacag tctgtaccgt ctgcggtatg tggaaaggtt atggttgtag ttgtgatcaa 13500

ctccgcgaac ccatgcttca gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg 13560

taagtgcagc ccgtcttaca ccgtgcggca caggcactag tactgatgtc gtatatagag 13620

cttttgacat ctacaatgat aaagtagctg gttttgctaa gttcctaaaa actaattgtt 13680

gtcgcttcca agaaaaggac gaagatgaca atctcattga ttcttacttt gtagttaaga 13740

gacacacttt ctctaactac caacatgaag aaacaattta caacctgctt aaggattgtc 13800

cagctgttgc taaacatgac ttctttaagt ttagaataga cggtgacatg gtaccacata 13860

tatcacgtca acgtcttact aaatacacaa tggcagacct cgtctatgct ttaaggcatt 13920

ttgatgaagg taattgtgac acattaaaag aaatacttgt cacatacaat tgttgtgatg 13980

atgactactt caataaaaag gactggtatg attttgtaga aaacccagat atattacgcg 14040

tatacgccaa cttaggtgaa cgtgtacgcc aagctttgtt aaaaacagta cagttctgtg 14100

atgccatgcg aaatgctggt attgttggtg tactgacatt agataatcaa gatctcaatg 14160

gtaactggta tgactttggt gatttcatac aaaccacgcc aggtagtgga gttcctgttg 14220

tagactctta ttattcattg ctcatgccta tattaacctt gaccagggct ttaactgcag 14280

agtcacatgt tgacactgac ttaacaaagc cttacattaa gtgggatttg ttaaaatacg 14340

acttcacgga agagaggtta aaactctttg accgttattt taaatactgg gatcagacat 14400

accacccaaa ttgtgttaac tgtttggatg acagatgcat tctgcattgt gcaaacttta 14460

atgttctgtt ctctacagtg ttcccaccta caagttttgg accactagtg agaaaaatat 14520

ttgttgatgg tgttccattt gtagtttcaa ctggatacca cttcagagag ctaggtgttg 14580

tacataatca ggatgtaaac ttacatagct ctagacttag ttttaaggaa ttacttgtgt 14640

atgctgctga tcctgctatg catgctgctt ctggtaatct attactagat aaacgcacta 14700

cgtgcttttc agtagctgca cttactaaca atgttgcttt tcaaactgtc aaacccggta 14760

attttaacaa ggacttctat gactttgctg tgtctaaggg tttctttaag gaaggaagtt 14820

ctgttgaatt aaaacacttc ttctttgctc aggatggtaa tgctgctatc agcgattatg 14880

actactatcg ttataatcta ccaacaatgt gtgatatcag acaactacta tttgtagttg 14940

aagttgttga taagtacttt gattgttacg atggtggctg tattaatgct aaccaagtca 15000

tcgtcaacaa cctagacaaa tcagctggtt ttccatttaa taaatggggt aaggctagac 15060

tttattatga ttccatgagt tatgaggatc aagatgcact tttcgcatat acaaaacgta 15120

atgtcatccc tactataact caaatgaacc ttaagtatgc cattagtgca aagaatagag 15180

ctcgcaccgt agctggtgtc tctatctgta gtactatgac caatagacag tttcatcaaa 15240

aattactcaa gtcaatagcc gccactagag gagctactgt agtaattgga acaagcaaat 15300

tctatggtgg ttggcacaac atgctcaaaa ctgtttatag tgatgtagaa aaccctcacc 15360

ttatgggttg ggattatcct aaatgtgata gagccatgcc taacatgctt agaattatgg 15420

cctcacttgt tcttgctcgc aaacatacaa cgtgttgtag cttgtcacac cgtttctata 15480

gattagctaa tgagtgtgct caagtattga gtgaaatggt catgtgtggc ggttcactat 15540

atgttaaacc aggtggaacc tcatcaggag atgccacaac tgcttatgct aatagtgtgt 15600

ttaacatttg tcaagctgtc acggccaatg ttaatgcact tttatctact gatggtaaca 15660

aaattgccga taagtatgtc cgcaatttac aacacagact ttatgagtgt ctctatagaa 15720

atagagatgt tgacacagac tttgtgaatg agttttacgc atatttgcgt aaacatttct 15780

caatgatgat actctctgac gatgctgttg tgtgtttcaa tagcacttat gcatctcaag 15840

gtctagtggc tagcataaag aactttaagt cagttcttta ctatcaaaac aacgttttta 15900

tgtctgaagc aaaatgttgg actgagactg accttactaa aggacctcat gaattttgct 15960

ctcaacatac aatgctagtt aaacagggtg atgattatgt gtaccttcct tacccagatc 16020

catcaagaat cctaggtgcc ggttgttttg tagatgatat cgtaaaaaca gatggtacac 16080

ttatgattga acggttcgtg tctttagcta tagatgctta cccacttact aaacatccta 16140

atcaggagta tgctgatgtc tttcatttgt acttacaata catacgtaag ctacatgatg 16200

agttaacagg acacatgtta gacatgtatt ctgttatgct tactaatgat aacacttcaa 16260

ggtattggga acctgagttt tatgaggcta tgtacacacc gcatacagtc ttacaagctg 16320

ttggtgcttg tgttctttgc aattcacaga cttcattaag atgtggtgct tgcatacgta 16380

gaccattctt atgttgtaaa tgctgttacg accatgtcat ctcaacatca cataaattag 16440

tcttgtctgt taatccgtat gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc 16500

aactttactt aggaggtatg agctattact gtaagtcaca taaaccaccc attagttttc 16560

cattgtgtgc taatggacaa gtttttggtc tctacaagaa tacatgtgtt ggtagcgata 16620

atgttactga ctttaatgca attgcaacat gtgactggac aaatgctggt gattacattt 16680

tagctaacac ctgtactgaa agactcaagc tttttgcagc agaaacgctc aaagctactg 16740

aggagacatt taaactgtct tatggtattg ctactgtacg tgaagtgctg tctgacagag 16800

aattacatct ttcatgggaa gttggtaaac ctagaccacc acttaaccga aattatgtct 16860

ttactggtta tcgtgtaact aaaaacagta aagtgcaaat cggagagtac acctttgaaa 16920

aaggtgacta tggtgatgct gttgtttacc gaggtacaac aacttacaaa ctcaacgttg 16980

gtgattattt tgtgctgaca tcacatacag taatgccatt aagtgcacct acactagtgc 17040

cacaagagca ctatgttaga attactggct tatacccaac actcaatatc tcagatgagt 17100

tttctagcaa tgttgcaaat tatcaaaagg ttggtatgca aaagtattct acactccagg 17160

gaccacctgg tactggtaaa agtcattttg ctattggtct agctctctac tacccttctg 17220

ctcgcatagt atatacagct tgctctcatg cagctgttga tgcactatgt gagaaggcat 17280

taaaatattt gcccatagac aaatgtagta gaattatacc tgcacgtgct cgtgtagagt 17340

gttttgataa attcaaggtg aattcaacat tagaacagta tgtcttttgt actgtaaatg 17400

cattgcctga gacgacagca gatatagttg tctttgatga aatttcaatg gccacaaatt 17460

atgatttgag tgttgtcaat gccagattac gtgctaagca ctatgtgtac attggtgatc 17520

ctgctcaatt acctgcacca cgcacattac taactaaggg tacactagaa ccagaatatt 17580

tcaattcagt gtgtagactt atgaaaacta taggtccaga catgttcctc ggaacttgtc 17640

gtagatgtcc tgctgaaatt gttgacactg tgagtgcttt ggtttatgat aataagctta 17700

aggcacataa agacaaatca gctcaatgct ttaaaatgtt ctacaagggt gttatcacgc 17760

atgatgtttc atctgcaatt aacaggccac aaataggcgt ggtaagagaa ttccttacac 17820

gtaaccctgc ttggagaaaa gctgtcttta tttcacctta caattcccag aatgctgtag 17880

cctcaaagat tttgggacta ccaactcaaa ctgttgattc atcacagggc tcagaatatg 17940

actatgtcat attcactcaa accactgaaa cagctcactc ttgtaatgta aacagattca 18000

acgttgctat taccagagca aaagtaggca tactttgcat aatgtctgat agagaccttt 18060

atgacaagtt gcaatttaca agtcttgaaa ttccacgtag gaatgtggca actttacaag 18120

ctgaaaatgt aacaggactc tttaaagatt gtagtaaggt aatcactggg ttacatccta 18180

cacaggcacc tacacactta agtgttgata ctaaattcaa aactgaaggt ttatgtgttg 18240

acatacctgg catacctaag gacatgacct atagaagatt aatctctatg atgggtttca 18300

aaatgaatta ccaggttaat ggttacccta acatgtttat cacccgcgaa gaagctataa 18360

gacatgtacg tgcatggatt ggcttcgatg tcgaaggttg tcatgctact agagaagctg 18420

ttggtaccaa tttaccttta cagctaggtt tttctacagg tgttaaccta gttgctgtac 18480

ctacaggtta tgttgataca cctaataata cagatttttc cagagttagt gctaaaccac 18540

cgcctggaga tcaatttaaa cacctcatac cacttatgta caaaggactt ccttggaatg 18600

tagtgcgtat aaagattgtc caaatgttaa gtgacacact taaaaatctc tctgacagag 18660

tcgtatttgt cttatgggca catggctttg agttgacatc tatgaagtat tttgtgaaga 18720

tcggacctga gcgcacatgt tgtctatgtg atagacgtgc tacatgcttt tccactgctt 18780

cagacactta tgcctgttgg catcattcta ttggatttga ttacgtctat aatccgttta 18840

tgattgatgt tcaacaatgg ggttttacag gtaacctaca aagcaaccat gatctgtatt 18900

gtcaagtcca tggtaatgca catgtagcta gttgtgatgc aatcatgact aggtgtctag 18960

ctgtccacga gtgctttgtt aagcgtgttg actggactat tgaatatcct ataatcggtg 19020

atgaactgaa gattaatgcg gcttgtagaa aggttcaaca catggttgtt aaagctgcat 19080

tattagcaga caaattccca gttcttcacg acattggtaa ccctaaagct attaagtgtg 19140

tacctcaagc tgatgtagaa tggaagttct atgatgcaca gccttgtagt gacaaagctt 19200

acaaaataga agaactgttc tattcttatg ccacacattc tgacaaattc acagatggtg 19260

tatgcctatt ttggaattgc aatgtcgata gatatcctgc taattccatt gtttgtagat 19320

ttgacactag agtgctatct aaccttaact tgcctggttg tgatggtggc agtttgtatg 19380

taaataagca tgcattccac acaccagctt ttgataaaag tgcttttgtt aatctaaagc 19440

aacttccatt tttctattac tctgacagtc catgtgagtc tcatggaaaa caagtagtgt 19500

cagatataga ttatgtacca ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg 19560

gtgctgtctg tagacatcat gctaatgagt acagattgta tctcgatgct tataacatga 19620

tgatctcagc tggctttagc ttgtgggttt acaaacaatt tgatacctat aacctctgga 19680

acacttttac aagacttcag agtttagaaa atgtggcttt taatgttgta aataagggac 19740

actttgatgg acaacagggt gaagtaccag tttctatcat taacaacact gtttacacaa 19800

aagttgatgg tgttgatgta gaattgtttg agaacaaaac cacattacct gttaatgtag 19860

catttgagct ttgggctaag cgcaacatta aaccagtacc agaggtgaaa atactcaata 19920

atttgggtgt ggacattgct gctaatactg tgatctggga ctacaaaaga gatgctccag 19980

cacatatatc tactattggt gtttgttcta tgactgacat agccaagaaa ccaactgaaa 20040

cgatttgtgc accactcact gtcttttttg atggtagagt tgatggtcaa gtagacttat 20100

ttagaaatgc ccgtaatggt gttcttatta cagaaggtag tgttaaaggt ttacaaccat 20160

ctgtaggtcc caaacaagct agtcttaatg gagtcacatt aattggagaa gccgtaaaaa 20220

cacagttcaa ttattacaag aaagtggatg gtgttgtcca acaattacct gaaacttact 20280

ttactcagag tagaaactta caggaattta agcccaggag tcaaatggaa attgatttct 20340

tagaacttgc tatggatgaa ttcattgaac ggtataaatt agaaggctat gccttcgaac 20400

atatcgttta tggagatttt agtcatagtc agttaggtgg tttacatcta ctgattggac 20460

tagctaaacg ttttaaggaa tcaccttttg aacttgaaga ttttattcct atggacagta 20520

cagttaaaaa ctacttcata acagatgcgc aaacaggttc atctaagtgt gtgtgttctg 20580

ttattgatct tttacttgat gacttcgttg aaataataaa gtcccaagat ttatctgtag 20640

tttctaaggt tgtcaaagtg actattgact atacagaaat ctcatttatg ctttggtgta 20700

aagatggcca tgtagaaaca ttttacccaa aattacaatc tagtcaagcg tggcaaccgg 20760

gtgttgctat gcctaatctt tacaaaatgc aaagaatgct attagaaaag tgtgaccttc 20820

aaaattatgg tgatagtgca acattaccta aaggcataat gatgaatgtc gcaaaatata 20880

ctcaactgtg tcaatattta aacacactga cattagctgt accctataat atgagagtta 20940

tccattttgg tgctggttct gataaaggag ttgcaccagg tacagctgtt ttaagacaat 21000

ggttgcctac aggtacgctg cttgtcgatt cagatcttaa tgactttgtc tctgatgcag 21060

attcaacttt gattggtgat tgtgcaactg tacatacagc taataaatgg gatctcatta 21120

ttagtgatat gtacgaccct aagactaaga atgtcacaaa agaaaacgac tctaaagagg 21180

gttttttcac ttacatttgt gggtttatac aacaaaagct agctcttgga ggttccgtgg 21240

ctataaagat aacagaacat tcttggaatg ctgatcttta taagctcatg ggacacttcg 21300

catggtggac agcctttgtt actaatgtga atgcgtcatc atctgaagca tttttaatcg 21360

gatgtaacta ccttggcaaa ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt 21420

acatattttg gaggaataca aatccaattc agctttcttc ttattcttta ttcgacatga 21480

gtaaattccc ccttaaatta aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca 21540

atgatatgat tctctctctt cttagtaaag gtagacttat aattagagaa aacaacagag 21600

ttgttatttc tagtgatgtt cttgttaaca actaaacgaa caatgtttgt ttttcttgtt 21660

ttattgccac tagtctctag tcagtgtgtt aatcttacaa ccagaactca attaccccct 21720

gcatacacta attctttcac acgtggtgtt tattaccctg acaaagtttt cagatcctca 21780

gttttacatt caactcagga cttgttctta cctttctttt ccaatgttac ttggttccat 21840

gctatacatg tctctgggac caatggtact aagaggtttg ataaccctgt cctaccattt 21900

aatgatggtg tttactttgc ttccactgag aagtctaaca taataagagg ctggattttt 21960

ggtactactt tagattcgaa aacccagtcc ctacttattg ttaataacgc tactaatgtt 22020

gttatcaaag tctgtgaatt tcaattttgt aacgatccat ttttgggtgt ttattaccac 22080

aaaaacaaca aaagttggat ggaaagtgag ttcagagttt attctagtgc gaataattgc 22140

acttttgaat acgtctctca gccttttctt atggaccttg aaggaaaaca gggtaatttc 22200

aaaaatctta gggaatttgt gttcaagaat attgatggtt acttcaagat atactctaag 22260

cacacgccta ttaatttagt gcgtgatctc cctcagggtt tttcggcttt agaaccattg 22320

gtagatttgc caataggtat taacatcact aggtttcaaa ctttacttgc tttacataga 22380

agttatttaa ctcctggtga ttcttcttca ggttggacag ctggtgctgc agcttattat 22440

gtgggttatc ttcaacctag gacttttcta ctgaagtaca atgaaaatgg aaccattaca 22500

gatgctgtag actgtgcact tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc 22560

actgtagaaa aaggaatcta tcaaacttct aactttagag tccaaccaac agaatctatt 22620

gttagatttc ctaacatcac aaacttgtgc ccttttggtg aagtttttaa cgccaccaga 22680

tttgcatctg tttatgcttg gaacaggaag agaatcagca actgtgttgc tgattattct 22740

gtcctgtata attccgcatc attttccact tttaagtgtt atggagtgtc tcctactaaa 22800

ttaaatgatc tctgctttac taatgtctat gcagattcat ttgtaattag aggtgatgaa 22860

gtcagacaaa tcgctccagg gcaaactgga aagattgctg attataacta caaattacca 22920

gatgatttta caggctgcgt tatagcttgg aattctaaca atcttgattc taaggttggt 22980

ggtaattata attacctgta cagattgttt aggaagtcta atctcaaacc ttttgagaga 23040

gatatttcaa ctgaaatcta tcaggccggt agcacacctt gtaatggtgt tgaaggtttt 23100

aattgttact ttcctctgca atcatatggt ttccaaccca ctaatggtgt tggttaccaa 23160

ccatacagag tagtagtact ttcttttgaa cttctacatg caccagcaac tgtttgtgga 23220

cctaaaaagt ctactaattt ggttaagaac aagtgtgtca atttcaactt caatggttta 23280

acaggcacag gtgttcttac tgagtctaac aaaaagtttc tgcctttcca acaatttggc 23340

agagacattg ctgacactac tgatgctgtt cgtgatccac aaacacttga gattcttgac 23400

attacaccat gttcttttgg tggtgtcagt gttataacac caggaacaaa tacttctaac 23460

caggttgctg ttctttatca ggatgttaac tgcacagaag tccctgttgc tattcatgca 23520

gatcaactta ctcctacttg gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt 23580

gcaggctgtt taataggggc tgaacatgtc aacaactcat atgagtgtga catacccatt 23640

ggtgcaggta tatgcgctag ttatcagact cagactaatt ctcctcggag agcaagaagt 23700

gtagctagtc aatccatcat tgcctacact atgtcacttg gtgcagaaaa ttcagttgct 23760

tactctaata actctattgc catacccaca aattttacta ttagcgttac cacagaaatt 23820

ctaccagtgt ctatgaccaa gacatcagta gattgtacaa tgtacatttg tggtgattca 23880

actgaatgca gcaatctttt gttgcaatat ggcagttttt gtacacaatt aaaccgtgct 23940

ttaactggaa tagctgttga acaagacaaa aacacccaag aagtttttgc acaagtcaaa 24000

caaatttaca agacaccacc aattaaagat tttggcggtt ttaattttag ccagatactg 24060

ccagatccat caaaaccaag caagaggtca tttattgaag atctactgtt caacaaagtg 24120

acacttgcag atgctggctt catcaaacaa tatggtgatt gccttggtga tattgctgct 24180

agagacctca tttgtgcaca aaagtttaac ggccttactg ttttgccacc tttgctcaca 24240

gatgaaatga ttgctcaata cacttctgca ctgttagcag gtacaatcac ttctggttgg 24300

acttttggtg caggtgctgc attacaaata ccatttgcta tgcaaatggc ttataggttt 24360

aatggtattg gagttacaca gaatgttctc tatgagaacc aaaaattgat tgccaaccaa 24420

tttaatagtg ctattggcaa aattcaagac tcactttctt ccacagcaag tgcacttgga 24480

aaacttcaag atgtggtcaa ccaaaatgca caagctttaa acacgcttgt taaacaactt 24540

agctccaatt ttggtgcaat ttcaagtgtt ttaaacgaca tcctttcacg tcttgacaaa 24600

gttgaggctg aagtgcaaat tgataggttg atcacaggca gacttcaaag tttgcagaca 24660

tatgtgactc aacaattaat tagagctgca gaaatcagag cttctgctaa tcttgctgct 24720

actaaaatgt cagagtgtgt acttggacaa tcaaaaagag ttgacttttg cggaaagggc 24780

tatcatctta tgtcatttcc tcagtcagca cctcatggtg tcgtcttttt gcatgtgact 24840

tatgtccctg cacaagaaaa gaacttcaca actgctcctg ccatttgtca tgatggaaaa 24900

gcacactttc ctcgtgaagg tgtctttgtt tcaaatggca cacactggtt tgtaacacaa 24960

aggaattttt atgaaccaca aatcattact acagacaaca catttgtgtc tggtaactgt 25020

gatgttgtaa taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac 25080

tcattcaagg aggagcttga taaatacttc aagaaccata cctcaccaga tgttgattta 25140

ggtgacatct ctggcattaa tgcttcagtt gtaaacattc agaaagaaat cgaccgcctc 25200

aatgaggttg ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat 25260

gagcagtata taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc 25320

atagtaatgg tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc 25380

tgttgttctt gtggatcctg ctgcaaattt gacgaggacg actctgagcc agtgctcaaa 25440

ggagtcaaat tacattacac ataaacgaac ttatggattt gtttatgaga atcttcacaa 25500

ttggaactgt aactttgaag caaggtgaaa tcaaggatgc tactccttca gattttgtta 25560

gagctactgc aacgataccg atacaagcat cacttccttt cggatggctt attgttggcg 25620

ttgcacttct tgctgttttt cagagcgctt ccaaaatcat aaccctcaaa aagagatggc 25680

aactagcact ctccaagggt gttcactttg tttgcaactt gctgttgttg tttgtaacag 25740

tttactcaca tcttttgctt gttgctgctg gccttgaagc cccttttctc tatctttatg 25800

ctttagtcta cttcttgcag agtataaact ttgtacgcat aataatgagg ctttggcttt 25860

gctggaaatg ccgttccaaa aacccattac tttatgatgc caactatttt ctttgctggc 25920

atactaattg ttacgactat tgtatacctt acaatagtgt aacttcttca attgtcatta 25980

cttcaggtga tggcacaaca agtcctattt ctgaacatga ctaccagatt ggtggttata 26040

ctgaaaaatg ggaatctgga gtaaaagact gtgttgtatt acacagttac ttcacttcag 26100

actattacca gctgtactca actcaattga gtacagacac tggtgttgaa catgttacct 26160

tcttcatcta caataaaatc gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg 26220

acgtttcatc cggagttgtt aatccagtaa tggaaccaat ttatgatgaa ccgacgacga 26280

ctactagcgt gcctttgtaa gcacaagctg atgagtacga acttatggca gattccaacg 26340

gtactattac cgttgaggag ctgaaaaagc tccttgaaca atggaaccta gtaataggtt 26400

tcctattcct tacatggatt tgcctgctgc aatttgccta tgccaacagg aataggtttt 26460

tgtacatcat taagttgatt ttcctctggc tgttatggcc agtaacttta gcttgttttg 26520

tgcttgctgc tgtttacaga ataaattgga tcaccggtgg aattgctatt gcaatggctt 26580

gtcttgtagg attgatgtgg ctaagctact tcattgcttc tttcagactg tttgcgcgta 26640

cgcgttccat gtggtcattc aatccagaaa ctaacattct tctcaacgtg ccactccatg 26700

gaactattct gactagaccg cttctagaaa gtgaactcgt aatcggagct gttatccttc 26760

gtggacatct tcgtattgct ggacatcatc taggacgctg tgacatcaag gatctaccta 26820

aagaaatcac tgttgctaca tcacgaacgc tttcttatta caaattggga gcttcacagc 26880

gtgtagcagg tgattcaggt tttgctgcat atagtcgcta caggattggc aactataaat 26940

taaacacaga ccattccagt agcagtgaca atattgcttt gcttgtacag taagtgacaa 27000

cagatgtttc atctcgttga ctttcaggtt actatagcag agatattact aatcatcatg 27060

aggactttta aagtttccat ttggaatctt gattacatca taaacctcat aattaagaac 27120

ttaagcaagt cactaactga gaataaatat tctcaactag acgaggagca gccaatggag 27180

attgattaaa cgaacatgaa aattattctt ttcttggcac tgataacact cgctacttgt 27240

gagctttatc actaccaaga gtgtgttaga ggtacaacag tacttttaaa agaaccttgc 27300

tcgtcgggaa catacgaggg caattcacca tttcatcctc tagctgataa caaatttgca 27360

ctgacttgct ttagcactca atttgctttt gcttgtcctg acggcgtaaa acacgtctat 27420

cagttacgtg ccagatcagt ttcacctaaa ctgttcatca gacaagagga agttcaagaa 27480

ctttactctc caatttttct tattgttgcg gcaatagtgt ttataacact ttgcttcaca 27540

ctcaaaagaa agacagaatg attgaacttt cattaattga cttctatttg tgctttttag 27600

cctttctgct attccttgtt ttaattatgc ttattatctt ttggttctca cttgaactgc 27660

aagatcataa tgaaacttgt cacgcctaag acgttcgtgt tgttttagat ttcatctaaa 27720

cgaacaaact aaaatgtctg ataatggacc tcaaaatcag cgaaatgcac ctcgcattac 27780

gtttggtgga ccatcagatt caactggcag taaccagaat ggagaacgaa gtggtgcgcg 27840

atcaaaacaa cgccgcccgc aaggtttacc caataatact gcgtcttggt tcaccgctct 27900

cactcaacat ggcaaggaag atttaaaatt ccctcgagga caaggcgttc caattaacac 27960

caatagcagt ccagatgacc aaattggcta ctaccgccgc gccacaagac gaattcgtgg 28020

tggtgatggt aaaatgaaag atctcagtcc aagatggtat ttctactatc taggaactgg 28080

gccagaagct ggacttcctt atggtgctaa caaagatggc atcatatggg ttgcaactga 28140

gggagccttg aatacaccaa aagatcacat tggcaccaga aatcctgcta acaatgctgc 28200

aatcgtgcta caacttcctc aaggaacaac attaccaaaa ggtttttacg cagaagggtc 28260

tagaggtgga agtcaagcct cttctagatc atcatcacgt agtcgcaaca gttcaagaaa 28320

ttcaactcca ggttcaagta gaggaacttc tcctgctaga atggctggaa atggaggtga 28380

tgctgctctt gctttgttac tacttgacag attgaaccag cttgagagca aaatgtctgg 28440

taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 28500

gaagcctaga caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 28560

acgtggtcca gaacaaactc aaggaaattt tggggatcag gaactaatca gacaaggaac 28620

tgattacaaa cattggccgc aaattgcaca atttgctcct tctgcttcag cgttctttgg 28680

aatgtcgaga attggaatgg aagtcacacc ttcgggaaca tggttgacct atacaggtgc 28740

catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 28800

tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 28860

tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 28920

tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 28980

aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29040

ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29100

acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29160

gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt 29220

acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29280

tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac aaaaaaaaac 29340

aaaaaaaa 29348

<210> 43

<211> 29152

<212> DNA

<213> Artificial sequence

<220>

<223> Synthesis of optimized sequence E-protein ORF6, and ORF8 triple deletions

<400> 43

caaatataac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 60

taatacgact cactataggg attaaaggtt tataccttcc caggtaacaa accaaccaac 120

tttcgatctc ttgtagatct gttctctaaa cgaactttaa aatctgtgtg gctgtcactc 180

ggctgcatgc ttagtgcact cacgcagtat aattaataac taattactgt cgttgacagg 240

acacgagtaa ctcgtctatc ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga 300

tcatcagcac atctaggttt cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc 360

cctggtttca acgagaaaac acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac 420

gtgttagtac gtggttttgg agattcagtg gaagaagtct tatcagaggc acgtcaacat 480

cttaaagatg gcacttgtgg cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa 540

cagccctatg tgttcatcaa acgttctgat gctagaactg cacctcatgg tcatgttatg 600

gttgagctgg tagcagaatt agaaggtatt cagtacggtc gtagtggtga gacattaggt 660

gttttagttc ctcatgtggg cgaaatacca gtggcttacc gcaaagttct tcttagaaag 720

aacggtaata aaggagctgg tggccatagt tacggcgctg atttaaagtc atttgactta 780

ggcgacgagc ttggcactga tccttatgaa gatttccaag aaaactggaa cactaaacat 840

agcagtggtg ttacccgtga actcatgcgt gagttaaatg gaggtgcata cactcgctat 900

gtcgataaca acttctgtgg acctgatggt taccctcttg agtgcattaa agaccttcta 960

gcacgtgctg gtaaagcttc atgcactttg tccgaacaac tggactttat tgacactaag 1020

aggggtgtat actgctgccg tgaacatgag catgaaattg cttggtacac ggaacgttct 1080

gaaaagagct atgaattgca gacacctttt gaaattaaac tggcaaagaa atttgacacc 1140

ttcaatgggg aatgtccaaa ttttgtattt cccctcaatt ccataatcaa gactattcaa 1200

ccaagggttg aaaagaaaaa gcttgatggc tttatgggta gaattcgatc tgtctatcca 1260

gttgcgtcac caaatgaatg caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat 1320

tgtggtgaaa cttcatggca gacgggcgat tttgttaaag ccacttgcga attttgtggc 1380

actgagaatt tgactaaaga aggtgccact acttgtggtt acttacccca aaatgctgtt 1440

gttaaaattt actgtccagc atgtcacaat tcagaagtag gacctgagca tagtcttgcc 1500

gaataccata atgaatctgg cttgaaaacc attcttcgta agggtggtcg cactattgct 1560

tttggaggat gtgtgttctc ttatgttggt tgccataaca agtgtgctta ttgggttcca 1620

cgtgcttcag ctaacatagg ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt 1680

cttaatgaca accttcttga aatactccaa aaagagaaag tcaacatcaa tattgttggt 1740

gactttaaac ttaatgaaga gatcgccatt attttggcat ctttttctgc ttccacaagt 1800

gcttttgtgg aaactgtgaa aggtttggat tataaagcat tcaaacagat tgttgaatcc 1860

tgtggtaatt ttaaggttac aaagggaaaa gctaaaaaag gtgcctggaa tattggtgaa 1920

cagaaatcaa tactgagtcc tctttatgca tttgcatcag aggctgctcg tgttgtacga 1980

tcaattttct cccgcactct tgaaactgct caaaattctg tgcgtgtttt acagaaggcc 2040

gctataacaa tactagatgg aatttcacag tattcactga gactcattga tgctatgatg 2100

ttcacatctg atttggctac taacaatcta gttgtaatgg cctacattac aggtggtgtt 2160

gttcagttga cttcgcagtg gctaactaac atctttggca ctgtttatga aaaactcaaa 2220

cccgtccttg attggcttga agagaagttt aaggaaggtg tagagtttct tagagacggt 2280

tgggagattg ttaaattcat ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc 2340

acctgtgcta aggaaattaa ggagagtgtt cagacattct ttaagcttgt aaacaagttt 2400

ttggctttgt gtgctgactc tatcattatt ggtggagcta aacttaaagc cttgaattta 2460

ggtgaaacat ttgtcacgca ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa 2520

gaaactggcc tactcatgcc tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa 2580

acacttccca cagaagtgtt aacagaggaa gttgtcttga aaactggtga tttacaacca 2640

ttagaacaac ctactagtga agctgttgaa gctccattgg ttggtacacc agtttgtatt 2700

aacgggctta tgttgctcga aatcaaagac acagaaaagt actgtgccct tgcacctaat 2760

atgatggtaa caaacaatac cttcacactc aaaggcggtg caccaacaaa ggttactttt 2820

ggtgatgaca ctgtgataga agtgcaaggt tacaagagtg tgaatatcac ttttgaactt 2880

gatgaaagga ttgataaagt acttaatgag aagtgctctg cctatacagt tgaactcggt 2940

acagaagtaa atgagttcgc ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca 3000

gtatctgaat tacttacacc actgggcatt gatttagatg agtggagtat ggctacatac 3060

tacttatttg atgagtctgg tgagtttaaa ttggcttcac atatgtattg ttctttctac 3120

cctccagatg aggatgaaga agaaggtgat tgtgaagaag aagagtttga gccatcaact 3180

caatatgagt atggtactga agatgattac caaggtaaac ctttggaatt tggtgccact 3240

tctgctgctt tacaacctga agaagaacaa gaagaagatt ggttagatga tgatagtcaa 3300

caaactgttg gtcaacaaga cggcagtgag gacaatcaga caactactat tcaaacaatt 3360

gttgaggttc aacctcaatt agagatggaa cttacaccag ttgttcagac tattgaagtg 3420

aatagtttta gtggttatct taaacttact gacaatgtat acatcaagaa tgcagacatt 3480

gtggaagaag ctaaaaaggt aaaaccaaca gtggttgtta atgcagccaa tgtttacctt 3540

aaacatggag gaggtgttgc aggagcctta aataaggcta ctaacaatgc catgcaagtt 3600

gaatctgatg attacatagc tactaatgga ccacttaaag tgggtggtag ttgtgtttta 3660

agcggacaca atcttgctaa acactgttta catgttgtcg gcccaaatgt taacaaaggt 3720

gaagatattc aacttcttaa gagtgcttat gaaaatttta accagcacga agttctactt 3780

gcaccattat tatcagctgg tatttttggt gctgacccta tacattcttt aagagtttgt 3840

gtagatactg ttcgcacaaa tgtctactta gctgtctttg ataaaaatct ctatgacaaa 3900

cttgtttcaa gctttttgga aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag 3960

attcctaaag aggaagttaa gccatttata actgaaagta aaccttcagt tgaacagaga 4020

aaacaagatg ataagaagat caaagcttgt gttgaagaag ttacaacaac tctggaagaa 4080

actaagttcc tcacagaaaa cttgctcctt tatatcgaca ttaatggcaa tcttcatcca 4140

gattctgcca ctcttgttag tgacattgac atcactttct taaagaaaga tgctccatat 4200

atagtgggtg atgttgttca agagggtgtt ttaactgctg tggttatacc tactaaaaag 4260

gctggtggca ctactgaaat gctagcgaaa gctttgagaa aagtgccaac agacaattat 4320

ataaccactt acccgggtca gggtttaaat ggttacactg tagaggaggc aaagacagtg 4380

cttaaaaagt gtaaaagtgc cttttacatt ctaccatcta ttatctctaa tgagaagcaa 4440

gaaattcttg gaactgtttc ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca 4500

cgcaaattaa tgcctgtctg tgtggaaact aaagccatag tttcaactat acagcgtaaa 4560

tataagggta tcaagataca agagggtgtg gttgattatg gtgctagatt ttacttttac 4620

accagtaaaa caactgtagc gtcacttatc aacacactta acgatctaaa tgaaactctt 4680

gttacaatgc cacttggcta tgtaacacat ggcttaaatt tggaagaagc tgctcggtat 4740

atgagatctc tcaaagtgcc agctacagtt tctgtttctt cacctgatgc tgttacagcg 4800

tataatggtt atcttacttc ttcttctaaa acacctgaag aacattttat tgaaaccatc 4860

tcacttgctg gttcctataa agattggtcc tattctggac aatctacaca actaggtata 4920

gaatttctta agagaggtga taaaagtgta tattacacgt ccaatcctac cacattccac 4980

ctagatggtg aagttatcac ctttgacaat cttaagacac ttctttcttt gagagaagtg 5040

aggactatta aggtgtttac aacagtagac aacattaacc tccacacgca agttgtggac 5100

atgtcaatga catatggaca acagtttggt ccaacttatt tggatggagc tgatgttact 5160

aagataaaac ctcataactc acatgaaggt aaaacatttt acgttttgcc taatgatgac 5220

actctacgtg ttgaggcttt tgagtactac cacacaactg atcctagttt tctgggtagg 5280

tacatgtcag cattaaatca cactaaaaag tggaaatacc cacaagttaa tggtttaact 5340

tcgattaaat gggcagataa caactgttat cttgccactg cattgttaac actccaacaa 5400

atagagttga agtttaatcc acctgctcta caagatgctt attacagagc aagggctggt 5460

gaagctgcta acttttgtgc acttatctta gcctactgta ataagacagt aggtgagtta 5520

ggtgatgtta gagaaacaat gagttacttg tttcaacatg ccaatttaga ttcttgcaaa 5580

agagtcttga acgtggtgtg taaaacttgt ggacaacagc agacaaccct taagggtgta 5640

gaagctgtta tgtacatggg cacactttct tatgaacaat tcaagaaagg tgttcagata 5700

ccttgtacgt gtggtaaaca agctacaaaa tatctagtac aacaggagtc accttttgtt 5760

atgatgtcag caccacctgc tcagtatgaa cttaagcatg gtacatttac ttgtgctagt 5820

gagtacactg gtaattacca gtgtggtcac tataagcata taacttctaa ggaaactttg 5880

tattgcatag acggtgcttt acttacaaag tcctcagaat acaaaggtcc tattacggat 5940

gttttctaca aagaaaacag ttacacaaca accataaaac cagttactta taagttggat 6000

ggtgttgttt gtacagaaat tgaccctaag ttggacaatt attataagaa ggacaactct 6060

tatttcacag agcaaccaat tgatcttgta ccaaaccaac catatccaaa cgcaagcttc 6120

gataatttta agttcgtatg cgataatatc aaatttgctg atgatctcaa ccagttaact 6180

ggttataaga aacctgcttc aagagagctt aaagttacat ttttccctga cttaaatggt 6240

gatgtggtgg ctattgatta taaacactac acaccctctt ttaagaaagg agctaaattg 6300

ttacataagc ctattgtttg gcatgttaac aatgcaacta ataaagccac gtataaacca 6360

aatacctggt gtatacgttg tctttggagc acaaaaccag ttgaaacatc aaattcgttt 6420

gatgtactga agtcagagga cgcgcaggga atggataatc ttgcatgtga agatctaaaa 6480

ccagtctctg aagaagtagt ggaaaatcct accatacaga aagacgttct tgagtgtaat 6540

gtgaaaacta ccgaagttgt aggagacatt atacttaaac cagcaaataa tagtttgaag 6600

atcacagaag aggttggcca cacagatcta atggctgctt atgtagacaa ttctagtctt 6660

actattaaga aacctaatga actctctaga gtattaggtt tgaaaaccct tgctactcat 6720

ggtttagctg ctgttaatag tgtcccttgg gatactatag ctaattatgc taagcctttt 6780

cttaacaaag ttgttagtac aactactaac atagttacac ggtgtcttaa tcgtgtttgt 6840

actaattata tgccttactt ctttacttta ttgctacaat tgtgtacttt tactagaagt 6900

acaaattcta gaatcaaggc atctatgccg actactatag caaagaatac tgttaagagt 6960

gtcggtaaat tttgtctaga ggcttcattt aattatctca agtcacctaa cttttctaag 7020

ctgataaaca ttatcatctg gtttttgcta ttaagtgttt gcctaggttc tttaatctac 7080

tcaaccgctg ctttaggtgt tttaatgtct aatttaggca tgccttctta ctgtactggt 7140

tacagagaag gctatttgaa ctctactaat gtcactattg caacctactg tactggatct 7200

ataccttgta gtgtttgtct tagtggttta gattctttag acacctatcc ttctcttgaa 7260

actatacaga ttaccatttc atctttcaaa tgggatttaa ctgcttttgg cttagttgca 7320

gagtggtttt tggcatatat tcttttcact aggtttttct atgtacttgg attggctgca 7380

atcatgcaat tgtttttcag ctattttgca gtccatttta ttagtaactc ttggcttatg 7440

tggcttataa ttaatcttgt gcagatggcc ccgatttcag ctatggttag aatgtacatc 7500

ttctttgcct cattttatta tgtgtggaaa agttatgtgc atgttgtaga cggttgtaat 7560

tcatcaactt gtatgatgtg ttacaaacgt aatagagcaa caagagtcga atgtacaact 7620

attgttaatg gtgttagaag gtccttttat gtctatgcta atggaggtaa aggcttttgc 7680

aaactacaca attggaattg tgttaattgt gatacattct gtgctggtag tacatttatt 7740

agtgatgaag ttgcgagaga cttgtcacta cagtttaaaa gaccaataaa tcctactgac 7800

caatcttctt acatcgttga tagtgttaca gtgaagaatg gttccatcca tctttacttt 7860

gataaagctg gtcaaaagac ttatgaaaga cattctctct ctcattttgt taacttagac 7920

aacctgagag ctaataacac taaaggttca ttgcctatta atgttatcgt tttcgacggt 7980

aaatcaaaat gtgaagaatc atctgcaaaa tcagcgtctg tttactacag tcagcttatg 8040

tgtcaaccta tactgttact agatcaggca ttagtgtctg atgttggtga tagtgcggaa 8100

gttgcagtta aaatgtttga tgcttacgtt aatacgtttt catcaacttt taacgtacca 8160

atggaaaaac tcaaaacact agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc 8220

ttagacaatg tcttatctac gtttatttca gcagctcggc aagggtttgt tgattcagat 8280

gtagaaacta aagatgttgt tgaatgtctt aaattgtcac atcaatctga catagaagtt 8340

actggcgata gttgtaataa ctatatgctc acctataaca aagttgaaaa catgacaccc 8400

cgtgaccttg gtgcttgtat tgactgtagt gctagacata ttaatgcgca ggtagcaaaa 8460

agtcacaaca ttgctttgat atggaacgtt aaagatttca tgtcattgtc tgaacaacta 8520

cgaaaacaaa tacgtagtgc tgctaaaaag aataacttac ccttcaagtt gacatgtgca 8580

actactagac aagttgttaa tgttgtaaca acaaagatag cacttaaggg tggtaaaatt 8640

gtgaataact ggttgaagca gcttattaaa gttacacttg tgttcctttt tgttgctgct 8700

attttctatc tgataacacc tgttcatgtc atgtctaaac atactgactt ttcaagtgaa 8760

atcataggat acaaggctat tgatggtggt gtcactcgtg acatagcatc tacagatact 8820

tgttttgcta acaaacatgc tgattttgac acatggttta gccagcgtgg tggtagttat 8880

actaatgaca aagcttgccc attgattgct gcagtcataa caagagaagt gggttttgtc 8940

gttcctggtt tgcctggaac gatattacgc acaactaatg gtgacttttt gcatttctta 9000

cctagagttt ttagtgcagt tggtaacatc tgttacacac catcaaaact tatagagtac 9060

actgactttg caacatcagc ttgtgttttg gctgctgaat gtacaatttt taaagacgct 9120

tctggtaagc cagtaccata ttgttatgat accaatgtac tagaaggttc tgttgcttat 9180

gaaagtttac gccctgacac acgttatgtg ctcatggatg gctctattat tcaatttcct 9240

aacacctacc ttgaaggttc tgtaagagtg gtaacaactt ttgattctga gtactgtagg 9300

cacggcactt gtgaaagatc agaagctggt gtttgtgtat ctactagtgg tagatgggta 9360

cttaacaacg attattacag atctttacca ggagttttct gtggtgtaga tgctgtaaat 9420

ttgcttacta acatgtttac accactaatt caacctattg gtgctttgga catatcagca 9480

tctatagtag ctggtggtat tgtagctatc gtagtaacat gccttgccta ctattttatg 9540

aggtttagac gtgcttttgg tgaatacagt catgtagttg cctttaatac tctcctattc 9600

cttatgtcat tcactgtact ctgtttaaca ccagtttact cattcttacc tggtgtttat 9660

tctgttattt acctgtactt gacattttat ctgactaatg atgtttcttt tctcgcacat 9720

attcagtgga tggttatgtt cacaccttta gtacctttct ggataacaat tgcttacatc 9780

atttgtattt ccacaaagca tttctattgg ttctttagta attacctaaa gagacgtgta 9840

gtctttaatg gtgtttcctt tagtactttt gaagaagctg cgctgtgcac ctttttgtta 9900

aataaggaga tgtatctaaa gttgcgtagt gatgtgctat tacctcttac gcaatataat 9960

agatacttag ctctttataa caagtacaag tatttcagtg gagcaatgga tacaactagc 10020

tacagagaag ctgcttgttg tcatctcgca aaggctctca atgacttcag taactcaggt 10080

tctgatgttc tttaccaacc accacaaacc tctatcacct cagctgtttt gcagagtggt 10140

tttagaaaaa tggcattccc atctggtaaa gttgagggtt gtatggtaca agtaacttgt 10200

ggtacaacta cacttaacgg tctttggctt gatgacgtag tttactgtcc aagacatgtg 10260

atctgcacct ctgaagatat gcttaaccct aattatgaag atctactcat ccgtaagtct 10320

aatcataact tcttggtaca ggctggtaat gttcaactca gggttattgg acattctatg 10380

caaaattgtg tacttaagct taaggttgat acagccaatc ctaagacacc taagtataag 10440

tttgttcgca ttcaaccagg acagactttt tcagtgttag cttgttacaa tggttcacca 10500

tctggtgttt accaatgtgc tatgaggccc aatttcacta ttaagggttc attccttaat 10560

ggttcatgtg gtagtgttgg ttttaacata gattatgact gtgtctcttt ttgttacatg 10620

caccatatgg aattaccaac tggagttcat gctggcacag acttagaagg taacttttat 10680

ggaccttttg ttgacaggca aacagcacaa gcagctggta cagatacaac tattacagtt 10740

aatgttcttg cttggttgta cgctgctgtt ataaatggag acaggtggtt tctcaatcga 10800

tttaccacaa ctcttaatga ctttaacctt gtggctatga agtacaatta tgaacctcta 10860

acacaagacc atgttgacat actaggacct ctttctgctc aaactggaat tgccgtttta 10920

gatatgtgtg cttcattaaa agaacttctg caaaatggta tgaatggacg taccatattg 10980

ggtagtgctt tattagaaga tgagtttaca ccttttgatg ttgttagaca atgctcaggt 11040

gttactttcc aaagtgcagt gaaaagaaca atcaagggta cacaccactg gttgttactc 11100

acaattttga cttcactttt agttttagtc cagagtactc aatggtcttt gttctttttc 11160

ttctacgaaa atgccttttt accttttgct atgggtatta ttgctatgtc tgcttttgca 11220

atgatgtttg tcaaacataa gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc 11280

actgtagctt actttaatat ggtctacatg cctgctagtt gggtgatgcg tattatgaca 11340

tggttggata tggttgatac tagtttgtct ggttttaagc taaaagactg tgttatgtat 11400

gcatcagctg tagtgttact aatccttatg acagcaagaa ctgtgtatga tgatggtgct 11460

aggagagtgt ggacacttat gaatgtcttg acactcgttt ataaagttta ctatggcaac 11520

gctttagatc aagccatttc catgtgggct cttataatct ctgttacttc taactactca 11580

ggtgtagtta caactgtcat gtttttggcc agaggtattg tttttatgtg tgttgagtat 11640

tgccctattt tcttcataac tggtaataca cttcagtgta taatgctagt ctattgtttc 11700

ttaggctatt tttgtacttg ttacttcggc ctcttttgtt tactcaaccg ctactttaga 11760

ctgactcttg gtgtttatga ttacttagtg tctacacagg agtttagata tatgaattca 11820

cagggactac tcccacccaa gaatagcata gatgccttca aactcaacat taaattgttg 11880

ggtgttggtg gcaaaccttg tatcaaagta gccactgtac agtctaaaat gtcagatgta 11940

aagtgcacat cagtagtctt actctcagtt ttgcaacaac tcagagtaga atcatcatct 12000

aaattgtggg ctcaatgtgt ccagttacac aatgacattc tcttagctaa agatactact 12060

gaagcctttg aaaaaatggt ttcactactt tctgttttgc tttccatgca gggtgctgta 12120

gacataaaca agctttgtga agaaatgctg gacaacaggg caaccttaca agctatagcc 12180

tcagagttta gttcccttcc atcatatgca gcttttgcta ctgctcaaga agcttatgag 12240

caggctgttg ctaatggtga ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat 12300

gtggctaaat ctgaatttga ccgtgatgca gccatgcaac gtaagttgga aaagatggct 12360

gatcaagcta tgacccaaat gtataaacag gctagatctg aggacaagag ggcaaaagtt 12420

actagtgcta tgcagacaat gcttttcact atgcttagaa agttggataa tgatgcactc 12480

aacaacatta tcaacaatgc aagagatggt tgtgttccct tgaacataat acctcttaca 12540

acagcagcca aactaatggt tgtcatacca gactacaaca catataagaa tacgtgtgat 12600

ggtacaacat ttacttatgc atcagcattg tgggaaatcc aacaggttgt agatgcagat 12660

agtaaaattg ttcagcttag tgaaattagt atggacaatt cacctaattt agcatggcct 12720

cttattgtaa cagctttaag ggccaattct gctgtcaaat tacagaataa tgagcttagt 12780

cctgttgcac taagacaaat gtcttgtgct gccggtacta cacaaactgc ttgcactgat 12840

gacaatgcgt tagcttacta caacacaaca aagggaggta ggtttgtact tgcactgtta 12900

tccgatttac aggatttgaa atgggctaga ttccctaaga gtgatggaac tggtactatc 12960

tatacagaac tggaaccacc ttgtaggttt gttacagaca cacctaaagg tcctaaagtg 13020

aagtatcttt acttcatcaa aggattaaac aacctaaata gaggtatggt acttggtagt 13080

ttagctgcca cagtacgttt acaagctggt aatgcaacag aagttcctgc taattcaact 13140

gtactttctt tctgtgcttt tgctgtagat gctgctaaag cttacaaaga ttatctagct 13200

agtgggggac aaccaatcac taattgtgtt aagatgttgt gtacacacac tggtactggt 13260

caggcaataa cagttacacc ggaagccaat atggatcaag aatcctttgg tggtgcatcg 13320

tgttgtctgt actgccgttg tcatatagat catccaaatc ctaaaggatt ttgtgactta 13380

aaaggtaagt atgtacaaat acctacaact tgtgctaatg accctgtggg ttttacactt 13440

aaaaacacag tctgtaccgt ctgcggtatg tggaaaggtt atggttgtag ttgtgatcaa 13500

ctccgcgaac ccatgcttca gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg 13560

taagtgcagc ccgtcttaca ccgtgcggca caggcactag tactgatgtc gtatatagag 13620

cttttgacat ctacaatgat aaagtagctg gttttgctaa gttcctaaaa actaattgtt 13680

gtcgcttcca agaaaaggac gaagatgaca atctcattga ttcttacttt gtagttaaga 13740

gacacacttt ctctaactac caacatgaag aaacaattta caacctgctt aaggattgtc 13800

cagctgttgc taaacatgac ttctttaagt ttagaataga cggtgacatg gtaccacata 13860

tatcacgtca acgtcttact aaatacacaa tggcagacct cgtctatgct ttaaggcatt 13920

ttgatgaagg taattgtgac acattaaaag aaatacttgt cacatacaat tgttgtgatg 13980

atgactactt caataaaaag gactggtatg attttgtaga aaacccagat atattacgcg 14040

tatacgccaa cttaggtgaa cgtgtacgcc aagctttgtt aaaaacagta cagttctgtg 14100

atgccatgcg aaatgctggt attgttggtg tactgacatt agataatcaa gatctcaatg 14160

gtaactggta tgactttggt gatttcatac aaaccacgcc aggtagtgga gttcctgttg 14220

tagactctta ttattcattg ctcatgccta tattaacctt gaccagggct ttaactgcag 14280

agtcacatgt tgacactgac ttaacaaagc cttacattaa gtgggatttg ttaaaatacg 14340

acttcacgga agagaggtta aaactctttg accgttattt taaatactgg gatcagacat 14400

accacccaaa ttgtgttaac tgtttggatg acagatgcat tctgcattgt gcaaacttta 14460

atgttctgtt ctctacagtg ttcccaccta caagttttgg accactagtg agaaaaatat 14520

ttgttgatgg tgttccattt gtagtttcaa ctggatacca cttcagagag ctaggtgttg 14580

tacataatca ggatgtaaac ttacatagct ctagacttag ttttaaggaa ttacttgtgt 14640

atgctgctga tcctgctatg catgctgctt ctggtaatct attactagat aaacgcacta 14700

cgtgcttttc agtagctgca cttactaaca atgttgcttt tcaaactgtc aaacccggta 14760

attttaacaa ggacttctat gactttgctg tgtctaaggg tttctttaag gaaggaagtt 14820

ctgttgaatt aaaacacttc ttctttgctc aggatggtaa tgctgctatc agcgattatg 14880

actactatcg ttataatcta ccaacaatgt gtgatatcag acaactacta tttgtagttg 14940

aagttgttga taagtacttt gattgttacg atggtggctg tattaatgct aaccaagtca 15000

tcgtcaacaa cctagacaaa tcagctggtt ttccatttaa taaatggggt aaggctagac 15060

tttattatga ttccatgagt tatgaggatc aagatgcact tttcgcatat acaaaacgta 15120

atgtcatccc tactataact caaatgaacc ttaagtatgc cattagtgca aagaatagag 15180

ctcgcaccgt agctggtgtc tctatctgta gtactatgac caatagacag tttcatcaaa 15240

aattactcaa gtcaatagcc gccactagag gagctactgt agtaattgga acaagcaaat 15300

tctatggtgg ttggcacaac atgctcaaaa ctgtttatag tgatgtagaa aaccctcacc 15360

ttatgggttg ggattatcct aaatgtgata gagccatgcc taacatgctt agaattatgg 15420

cctcacttgt tcttgctcgc aaacatacaa cgtgttgtag cttgtcacac cgtttctata 15480

gattagctaa tgagtgtgct caagtattga gtgaaatggt catgtgtggc ggttcactat 15540

atgttaaacc aggtggaacc tcatcaggag atgccacaac tgcttatgct aatagtgtgt 15600

ttaacatttg tcaagctgtc acggccaatg ttaatgcact tttatctact gatggtaaca 15660

aaattgccga taagtatgtc cgcaatttac aacacagact ttatgagtgt ctctatagaa 15720

atagagatgt tgacacagac tttgtgaatg agttttacgc atatttgcgt aaacatttct 15780

caatgatgat actctctgac gatgctgttg tgtgtttcaa tagcacttat gcatctcaag 15840

gtctagtggc tagcataaag aactttaagt cagttcttta ctatcaaaac aacgttttta 15900

tgtctgaagc aaaatgttgg actgagactg accttactaa aggacctcat gaattttgct 15960

ctcaacatac aatgctagtt aaacagggtg atgattatgt gtaccttcct tacccagatc 16020

catcaagaat cctaggtgcc ggttgttttg tagatgatat cgtaaaaaca gatggtacac 16080

ttatgattga acggttcgtg tctttagcta tagatgctta cccacttact aaacatccta 16140

atcaggagta tgctgatgtc tttcatttgt acttacaata catacgtaag ctacatgatg 16200

agttaacagg acacatgtta gacatgtatt ctgttatgct tactaatgat aacacttcaa 16260

ggtattggga acctgagttt tatgaggcta tgtacacacc gcatacagtc ttacaagctg 16320

ttggtgcttg tgttctttgc aattcacaga cttcattaag atgtggtgct tgcatacgta 16380

gaccattctt atgttgtaaa tgctgttacg accatgtcat ctcaacatca cataaattag 16440

tcttgtctgt taatccgtat gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc 16500

aactttactt aggaggtatg agctattact gtaagtcaca taaaccaccc attagttttc 16560

cattgtgtgc taatggacaa gtttttggtc tctacaagaa tacatgtgtt ggtagcgata 16620

atgttactga ctttaatgca attgcaacat gtgactggac aaatgctggt gattacattt 16680

tagctaacac ctgtactgaa agactcaagc tttttgcagc agaaacgctc aaagctactg 16740

aggagacatt taaactgtct tatggtattg ctactgtacg tgaagtgctg tctgacagag 16800

aattacatct ttcatgggaa gttggtaaac ctagaccacc acttaaccga aattatgtct 16860

ttactggtta tcgtgtaact aaaaacagta aagtgcaaat cggagagtac acctttgaaa 16920

aaggtgacta tggtgatgct gttgtttacc gaggtacaac aacttacaaa ctcaacgttg 16980

gtgattattt tgtgctgaca tcacatacag taatgccatt aagtgcacct acactagtgc 17040

cacaagagca ctatgttaga attactggct tatacccaac actcaatatc tcagatgagt 17100

tttctagcaa tgttgcaaat tatcaaaagg ttggtatgca aaagtattct acactccagg 17160

gaccacctgg tactggtaaa agtcattttg ctattggtct agctctctac tacccttctg 17220

ctcgcatagt atatacagct tgctctcatg cagctgttga tgcactatgt gagaaggcat 17280

taaaatattt gcccatagac aaatgtagta gaattatacc tgcacgtgct cgtgtagagt 17340

gttttgataa attcaaggtg aattcaacat tagaacagta tgtcttttgt actgtaaatg 17400

cattgcctga gacgacagca gatatagttg tctttgatga aatttcaatg gccacaaatt 17460

atgatttgag tgttgtcaat gccagattac gtgctaagca ctatgtgtac attggtgatc 17520

ctgctcaatt acctgcacca cgcacattac taactaaggg tacactagaa ccagaatatt 17580

tcaattcagt gtgtagactt atgaaaacta taggtccaga catgttcctc ggaacttgtc 17640

gtagatgtcc tgctgaaatt gttgacactg tgagtgcttt ggtttatgat aataagctta 17700

aggcacataa agacaaatca gctcaatgct ttaaaatgtt ctacaagggt gttatcacgc 17760

atgatgtttc atctgcaatt aacaggccac aaataggcgt ggtaagagaa ttccttacac 17820

gtaaccctgc ttggagaaaa gctgtcttta tttcacctta caattcccag aatgctgtag 17880

cctcaaagat tttgggacta ccaactcaaa ctgttgattc atcacagggc tcagaatatg 17940

actatgtcat attcactcaa accactgaaa cagctcactc ttgtaatgta aacagattca 18000

acgttgctat taccagagca aaagtaggca tactttgcat aatgtctgat agagaccttt 18060

atgacaagtt gcaatttaca agtcttgaaa ttccacgtag gaatgtggca actttacaag 18120

ctgaaaatgt aacaggactc tttaaagatt gtagtaaggt aatcactggg ttacatccta 18180

cacaggcacc tacacactta agtgttgata ctaaattcaa aactgaaggt ttatgtgttg 18240

acatacctgg catacctaag gacatgacct atagaagatt aatctctatg atgggtttca 18300

aaatgaatta ccaggttaat ggttacccta acatgtttat cacccgcgaa gaagctataa 18360

gacatgtacg tgcatggatt ggcttcgatg tcgaaggttg tcatgctact agagaagctg 18420

ttggtaccaa tttaccttta cagctaggtt tttctacagg tgttaaccta gttgctgtac 18480

ctacaggtta tgttgataca cctaataata cagatttttc cagagttagt gctaaaccac 18540

cgcctggaga tcaatttaaa cacctcatac cacttatgta caaaggactt ccttggaatg 18600

tagtgcgtat aaagattgtc caaatgttaa gtgacacact taaaaatctc tctgacagag 18660

tcgtatttgt cttatgggca catggctttg agttgacatc tatgaagtat tttgtgaaga 18720

tcggacctga gcgcacatgt tgtctatgtg atagacgtgc tacatgcttt tccactgctt 18780

cagacactta tgcctgttgg catcattcta ttggatttga ttacgtctat aatccgttta 18840

tgattgatgt tcaacaatgg ggttttacag gtaacctaca aagcaaccat gatctgtatt 18900

gtcaagtcca tggtaatgca catgtagcta gttgtgatgc aatcatgact aggtgtctag 18960

ctgtccacga gtgctttgtt aagcgtgttg actggactat tgaatatcct ataatcggtg 19020

atgaactgaa gattaatgcg gcttgtagaa aggttcaaca catggttgtt aaagctgcat 19080

tattagcaga caaattccca gttcttcacg acattggtaa ccctaaagct attaagtgtg 19140

tacctcaagc tgatgtagaa tggaagttct atgatgcaca gccttgtagt gacaaagctt 19200

acaaaataga agaactgttc tattcttatg ccacacattc tgacaaattc acagatggtg 19260

tatgcctatt ttggaattgc aatgtcgata gatatcctgc taattccatt gtttgtagat 19320

ttgacactag agtgctatct aaccttaact tgcctggttg tgatggtggc agtttgtatg 19380

taaataagca tgcattccac acaccagctt ttgataaaag tgcttttgtt aatctaaagc 19440

aacttccatt tttctattac tctgacagtc catgtgagtc tcatggaaaa caagtagtgt 19500

cagatataga ttatgtacca ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg 19560

gtgctgtctg tagacatcat gctaatgagt acagattgta tctcgatgct tataacatga 19620

tgatctcagc tggctttagc ttgtgggttt acaaacaatt tgatacctat aacctctgga 19680

acacttttac aagacttcag agtttagaaa atgtggcttt taatgttgta aataagggac 19740

actttgatgg acaacagggt gaagtaccag tttctatcat taacaacact gtttacacaa 19800

aagttgatgg tgttgatgta gaattgtttg agaacaaaac cacattacct gttaatgtag 19860

catttgagct ttgggctaag cgcaacatta aaccagtacc agaggtgaaa atactcaata 19920

atttgggtgt ggacattgct gctaatactg tgatctggga ctacaaaaga gatgctccag 19980

cacatatatc tactattggt gtttgttcta tgactgacat agccaagaaa ccaactgaaa 20040

cgatttgtgc accactcact gtcttttttg atggtagagt tgatggtcaa gtagacttat 20100

ttagaaatgc ccgtaatggt gttcttatta cagaaggtag tgttaaaggt ttacaaccat 20160

ctgtaggtcc caaacaagct agtcttaatg gagtcacatt aattggagaa gccgtaaaaa 20220

cacagttcaa ttattacaag aaagtggatg gtgttgtcca acaattacct gaaacttact 20280

ttactcagag tagaaactta caggaattta agcccaggag tcaaatggaa attgatttct 20340

tagaacttgc tatggatgaa ttcattgaac ggtataaatt agaaggctat gccttcgaac 20400

atatcgttta tggagatttt agtcatagtc agttaggtgg tttacatcta ctgattggac 20460

tagctaaacg ttttaaggaa tcaccttttg aacttgaaga ttttattcct atggacagta 20520

cagttaaaaa ctacttcata acagatgcgc aaacaggttc atctaagtgt gtgtgttctg 20580

ttattgatct tttacttgat gacttcgttg aaataataaa gtcccaagat ttatctgtag 20640

tttctaaggt tgtcaaagtg actattgact atacagaaat ctcatttatg ctttggtgta 20700

aagatggcca tgtagaaaca ttttacccaa aattacaatc tagtcaagcg tggcaaccgg 20760

gtgttgctat gcctaatctt tacaaaatgc aaagaatgct attagaaaag tgtgaccttc 20820

aaaattatgg tgatagtgca acattaccta aaggcataat gatgaatgtc gcaaaatata 20880

ctcaactgtg tcaatattta aacacactga cattagctgt accctataat atgagagtta 20940

tccattttgg tgctggttct gataaaggag ttgcaccagg tacagctgtt ttaagacaat 21000

ggttgcctac aggtacgctg cttgtcgatt cagatcttaa tgactttgtc tctgatgcag 21060

attcaacttt gattggtgat tgtgcaactg tacatacagc taataaatgg gatctcatta 21120

ttagtgatat gtacgaccct aagactaaga atgtcacaaa agaaaacgac tctaaagagg 21180

gttttttcac ttacatttgt gggtttatac aacaaaagct agctcttgga ggttccgtgg 21240

ctataaagat aacagaacat tcttggaatg ctgatcttta taagctcatg ggacacttcg 21300

catggtggac agcctttgtt actaatgtga atgcgtcatc atctgaagca tttttaatcg 21360

gatgtaacta ccttggcaaa ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt 21420

acatattttg gaggaataca aatccaattc agctttcttc ttattcttta ttcgacatga 21480

gtaaattccc ccttaaatta aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca 21540

atgatatgat tctctctctt cttagtaaag gtagacttat aattagagaa aacaacagag 21600

ttgttatttc tagtgatgtt cttgttaaca actaaacgaa caatgtttgt ttttcttgtt 21660

ttattgccac tagtctctag tcagtgtgtt aatcttacaa ccagaactca attaccccct 21720

gcatacacta attctttcac acgtggtgtt tattaccctg acaaagtttt cagatcctca 21780

gttttacatt caactcagga cttgttctta cctttctttt ccaatgttac ttggttccat 21840

gctatacatg tctctgggac caatggtact aagaggtttg ataaccctgt cctaccattt 21900

aatgatggtg tttactttgc ttccactgag aagtctaaca taataagagg ctggattttt 21960

ggtactactt tagattcgaa aacccagtcc ctacttattg ttaataacgc tactaatgtt 22020

gttatcaaag tctgtgaatt tcaattttgt aacgatccat ttttgggtgt ttattaccac 22080

aaaaacaaca aaagttggat ggaaagtgag ttcagagttt attctagtgc gaataattgc 22140

acttttgaat acgtctctca gccttttctt atggaccttg aaggaaaaca gggtaatttc 22200

aaaaatctta gggaatttgt gttcaagaat attgatggtt acttcaagat atactctaag 22260

cacacgccta ttaatttagt gcgtgatctc cctcagggtt tttcggcttt agaaccattg 22320

gtagatttgc caataggtat taacatcact aggtttcaaa ctttacttgc tttacataga 22380

agttatttaa ctcctggtga ttcttcttca ggttggacag ctggtgctgc agcttattat 22440

gtgggttatc ttcaacctag gacttttcta ctgaagtaca atgaaaatgg aaccattaca 22500

gatgctgtag actgtgcact tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc 22560

actgtagaaa aaggaatcta tcaaacttct aactttagag tccaaccaac agaatctatt 22620

gttagatttc ctaacatcac aaacttgtgc ccttttggtg aagtttttaa cgccaccaga 22680

tttgcatctg tttatgcttg gaacaggaag agaatcagca actgtgttgc tgattattct 22740

gtcctgtata attccgcatc attttccact tttaagtgtt atggagtgtc tcctactaaa 22800

ttaaatgatc tctgctttac taatgtctat gcagattcat ttgtaattag aggtgatgaa 22860

gtcagacaaa tcgctccagg gcaaactgga aagattgctg attataacta caaattacca 22920

gatgatttta caggctgcgt tatagcttgg aattctaaca atcttgattc taaggttggt 22980

ggtaattata attacctgta cagattgttt aggaagtcta atctcaaacc ttttgagaga 23040

gatatttcaa ctgaaatcta tcaggccggt agcacacctt gtaatggtgt tgaaggtttt 23100

aattgttact ttcctctgca atcatatggt ttccaaccca ctaatggtgt tggttaccaa 23160

ccatacagag tagtagtact ttcttttgaa cttctacatg caccagcaac tgtttgtgga 23220

cctaaaaagt ctactaattt ggttaagaac aagtgtgtca atttcaactt caatggttta 23280

acaggcacag gtgttcttac tgagtctaac aaaaagtttc tgcctttcca acaatttggc 23340

agagacattg ctgacactac tgatgctgtt cgtgatccac aaacacttga gattcttgac 23400

attacaccat gttcttttgg tggtgtcagt gttataacac caggaacaaa tacttctaac 23460

caggttgctg ttctttatca ggatgttaac tgcacagaag tccctgttgc tattcatgca 23520

gatcaactta ctcctacttg gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt 23580

gcaggctgtt taataggggc tgaacatgtc aacaactcat atgagtgtga catacccatt 23640

ggtgcaggta tatgcgctag ttatcagact cagactaatt ctcctcggag agcaagaagt 23700

gtagctagtc aatccatcat tgcctacact atgtcacttg gtgcagaaaa ttcagttgct 23760

tactctaata actctattgc catacccaca aattttacta ttagcgttac cacagaaatt 23820

ctaccagtgt ctatgaccaa gacatcagta gattgtacaa tgtacatttg tggtgattca 23880

actgaatgca gcaatctttt gttgcaatat ggcagttttt gtacacaatt aaaccgtgct 23940

ttaactggaa tagctgttga acaagacaaa aacacccaag aagtttttgc acaagtcaaa 24000

caaatttaca agacaccacc aattaaagat tttggcggtt ttaattttag ccagatactg 24060

ccagatccat caaaaccaag caagaggtca tttattgaag atctactgtt caacaaagtg 24120

acacttgcag atgctggctt catcaaacaa tatggtgatt gccttggtga tattgctgct 24180

agagacctca tttgtgcaca aaagtttaac ggccttactg ttttgccacc tttgctcaca 24240

gatgaaatga ttgctcaata cacttctgca ctgttagcag gtacaatcac ttctggttgg 24300

acttttggtg caggtgctgc attacaaata ccatttgcta tgcaaatggc ttataggttt 24360

aatggtattg gagttacaca gaatgttctc tatgagaacc aaaaattgat tgccaaccaa 24420

tttaatagtg ctattggcaa aattcaagac tcactttctt ccacagcaag tgcacttgga 24480

aaacttcaag atgtggtcaa ccaaaatgca caagctttaa acacgcttgt taaacaactt 24540

agctccaatt ttggtgcaat ttcaagtgtt ttaaacgaca tcctttcacg tcttgacaaa 24600

gttgaggctg aagtgcaaat tgataggttg atcacaggca gacttcaaag tttgcagaca 24660

tatgtgactc aacaattaat tagagctgca gaaatcagag cttctgctaa tcttgctgct 24720

actaaaatgt cagagtgtgt acttggacaa tcaaaaagag ttgacttttg cggaaagggc 24780

tatcatctta tgtcatttcc tcagtcagca cctcatggtg tcgtcttttt gcatgtgact 24840

tatgtccctg cacaagaaaa gaacttcaca actgctcctg ccatttgtca tgatggaaaa 24900

gcacactttc ctcgtgaagg tgtctttgtt tcaaatggca cacactggtt tgtaacacaa 24960

aggaattttt atgaaccaca aatcattact acagacaaca catttgtgtc tggtaactgt 25020

gatgttgtaa taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac 25080

tcattcaagg aggagcttga taaatacttc aagaaccata cctcaccaga tgttgattta 25140

ggtgacatct ctggcattaa tgcttcagtt gtaaacattc agaaagaaat cgaccgcctc 25200

aatgaggttg ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat 25260

gagcagtata taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc 25320

atagtaatgg tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc 25380

tgttgttctt gtggatcctg ctgcaaattt gacgaggacg actctgagcc agtgctcaaa 25440

ggagtcaaat tacattacac ataaacgaac ttatggattt gtttatgaga atcttcacaa 25500

ttggaactgt aactttgaag caaggtgaaa tcaaggatgc tactccttca gattttgtta 25560

gagctactgc aacgataccg atacaagcat cacttccttt cggatggctt attgttggcg 25620

ttgcacttct tgctgttttt cagagcgctt ccaaaatcat aaccctcaaa aagagatggc 25680

aactagcact ctccaagggt gttcactttg tttgcaactt gctgttgttg tttgtaacag 25740

tttactcaca tcttttgctt gttgctgctg gccttgaagc cccttttctc tatctttatg 25800

ctttagtcta cttcttgcag agtataaact ttgtacgcat aataatgagg ctttggcttt 25860

gctggaaatg ccgttccaaa aacccattac tttatgatgc caactatttt ctttgctggc 25920

atactaattg ttacgactat tgtatacctt acaatagtgt aacttcttca attgtcatta 25980

cttcaggtga tggcacaaca agtcctattt ctgaacatga ctaccagatt ggtggttata 26040

ctgaaaaatg ggaatctgga gtaaaagact gtgttgtatt acacagttac ttcacttcag 26100

actattacca gctgtactca actcaattga gtacagacac tggtgttgaa catgttacct 26160

tcttcatcta caataaaatc gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg 26220

acgtttcatc cggagttgtt aatccagtaa tggaaccaat ttatgatgaa ccgacgacga 26280

ctactagcgt gcctttgtaa gcacaagctg atgagtacga acttatggca gattccaacg 26340

gtactattac cgttgaggag ctgaaaaagc tccttgaaca atggaaccta gtaataggtt 26400

tcctattcct tacatggatt tgcctgctgc aatttgccta tgccaacagg aataggtttt 26460

tgtacatcat taagttgatt ttcctctggc tgttatggcc agtaacttta gcttgttttg 26520

tgcttgctgc tgtttacaga ataaattgga tcaccggtgg aattgctatt gcaatggctt 26580

gtcttgtagg attgatgtgg ctaagctact tcattgcttc tttcagactg tttgcgcgta 26640

cgcgttccat gtggtcattc aatccagaaa ctaacattct tctcaacgtg ccactccatg 26700

gaactattct gactagaccg cttctagaaa gtgaactcgt aatcggagct gttatccttc 26760

gtggacatct tcgtattgct ggacatcatc taggacgctg tgacatcaag gatctaccta 26820

aagaaatcac tgttgctaca tcacgaacgc tttcttatta caaattggga gcttcacagc 26880

gtgtagcagg tgattcaggt tttgctgcat atagtcgcta caggattggc aactataaat 26940

taaacacaga ccattccagt agcagtgaca atattgcttt gcttgtacag taaacgaaca 27000

tgaaaattat tcttttcttg gcactgataa cactcgctac ttgtgagctt tatcactacc 27060

aagagtgtgt tagaggtaca acagtacttt taaaagaacc ttgctcgtcg ggaacatacg 27120

agggcaattc accatttcat cctctagctg ataacaaatt tgcactgact tgctttagca 27180

ctcaatttgc ttttgcttgt cctgacggcg taaaacacgt ctatcagtta cgtgccagat 27240

cagtttcacc taaactgttc atcagacaag aggaagttca agaactttac tctccaattt 27300

ttcttattgt tgcggcaata gtgtttataa cactttgctt cacactcaaa agaaagacag 27360

aatgattgaa ctttcattaa ttgacttcta tttgtgcttt ttagcctttc tgctattcct 27420

tgttttaatt atgcttatta tcttttggtt ctcacttgaa ctgcaagatc ataatgaaac 27480

ttgtcacgcc taagacgttc gtgttgtttt agatttcatc taaacgaaca aactaaaatg 27540

tctgataatg gacctcaaaa tcagcgaaat gcacctcgca ttacgtttgg tggaccatca 27600

gattcaactg gcagtaacca gaatggagaa cgaagtggtg cgcgatcaaa acaacgccgc 27660

ccgcaaggtt tacccaataa tactgcgtct tggttcaccg ctctcactca acatggcaag 27720

gaagatttaa aattccctcg aggacaaggc gttccaatta acaccaatag cagtccagat 27780

gaccaaattg gctactaccg ccgcgccaca agacgaattc gtggtggtga tggtaaaatg 27840

aaagatctca gtccaagatg gtatttctac tatctaggaa ctgggccaga agctggactt 27900

ccttatggtg ctaacaaaga tggcatcata tgggttgcaa ctgagggagc cttgaataca 27960

ccaaaagatc acattggcac cagaaatcct gctaacaatg ctgcaatcgt gctacaactt 28020

cctcaaggaa caacattacc aaaaggtttt tacgcagaag ggtctagagg tggaagtcaa 28080

gcctcttcta gatcatcatc acgtagtcgc aacagttcaa gaaattcaac tccaggttca 28140

agtagaggaa cttctcctgc tagaatggct ggaaatggag gtgatgctgc tcttgctttg 28200

ttactacttg acagattgaa ccagcttgag agcaaaatgt ctggtaaagg ccaacaacaa 28260

caaggccaaa ctgtcactaa gaaatctgct gctgaggctt ctaagaagcc tagacaaaaa 28320

cgtactgcca ctaaagcata caatgtaaca caagctttcg gcagacgtgg tccagaacaa 28380

actcaaggaa attttgggga tcaggaacta atcagacaag gaactgatta caaacattgg 28440

ccgcaaattg cacaatttgc tccttctgct tcagcgttct ttggaatgtc gagaattgga 28500

atggaagtca caccttcggg aacatggttg acctatacag gtgccatcaa attggatgac 28560

aaagatccaa atttcaaaga tcaagtcatt ttgctgaata agcatattga cgcatacaaa 28620

acattcccac caacagagcc taaaaaggac aaaaagaaga aggctgatga aactcaagcc 28680

ttaccgcaga gacagaagaa acagcaaact gtgactcttc ttcctgctgc agatttggat 28740

gatttctcca aacaattgca acaatccatg agcagtgctg actcaactca ggcctaaact 28800

catgcagacc acacaaggca gatgggctat ataaacgttt tcgcttttcc gtttacgata 28860

tatagtctac tcttgtgcag aatgaattct cgtaactaca tagcacaagt agatgtagtt 28920

aactttaatc tcacatagca atctttaatc agtgtgtaac attagggagg acttgaaaga 28980

gccaccacat tttcaccgag gccacgcgga gtacgatcga gtgtacagtg aacaatgcta 29040

gggagagctg cctatatgga agagccctaa tgtgtaaaat taattttagt agtgctatcc 29100

ccatgtgatt ttaatagctt cttaggagaa tgacaaaaaa aaacaaaaaa aa 29152

<210> 44

<211> 29968

<212> DNA

<213> Artificial sequence

<220>

<223> Synthesis optimized

<400> 44

caaatataac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 60

taatacgact cactataggg attaaaggtt tataccttcc caggtaacaa accaaccaac 120

tttcgatctc ttgtagatct gttctctaaa cgaactttaa aatctgtgtg gctgtcactc 180

ggctgcatgc ttagtgcact cacgcagtat aattaataac taattactgt cgttgacagg 240

acacgagtaa ctcgtctatc ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga 300

tcatcagcac atctaggttt cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc 360

cctggtttca acgagaaaac acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac 420

gtgttagtac gtggttttgg agattcagtg gaagaagtct tatcagaggc acgtcaacat 480

cttaaagatg gcacttgtgg cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa 540

cagccctatg tgttcatcaa acgttctgat gctagaactg cacctcatgg tcatgttatg 600

gttgagctgg tagcagaatt agaaggtatt cagtacggtc gtagtggtga gacattaggt 660

gttttagttc ctcatgtggg cgaaatacca gtggcttacc gcaaagttct tcttagaaag 720

aacggtaata aaggagctgg tggccatagt tacggcgctg atttaaagtc atttgactta 780

ggcgacgagc ttggcactga tccttatgaa gatttccaag aaaactggaa cactaaacat 840

agcagtggtg ttacccgtga actcatgcgt gagttaaatg gaggtgcata cactcgctat 900

gtcgataaca acttctgtgg acctgatggt taccctcttg agtgcattaa agaccttcta 960

gcacgtgctg gtaaagcttc atgcactttg tccgaacaac tggactttat tgacactaag 1020

aggggtgtat actgctgccg tgaacatgag catgaaattg cttggtacac ggaacgttct 1080

gaaaagagct atgaattgca gacacctttt gaaattaaac tggcaaagaa atttgacacc 1140

ttcaatgggg aatgtccaaa ttttgtattt cccctcaatt ccataatcaa gactattcaa 1200

ccaagggttg aaaagaaaaa gcttgatggc tttatgggta gaattcgatc tgtctatcca 1260

gttgcgtcac caaatgaatg caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat 1320

tgtggtgaaa cttcatggca gacgggcgat tttgttaaag ccacttgcga attttgtggc 1380

actgagaatt tgactaaaga aggtgccact acttgtggtt acttacccca aaatgctgtt 1440

gttaaaattt actgtccagc atgtcacaat tcagaagtag gacctgagca tagtcttgcc 1500

gaataccata atgaatctgg cttgaaaacc attcttcgta agggtggtcg cactattgct 1560

tttggaggat gtgtgttctc ttatgttggt tgccataaca agtgtgctta ttgggttcca 1620

cgtgcttcag ctaacatagg ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt 1680

cttaatgaca accttcttga aatactccaa aaagagaaag tcaacatcaa tattgttggt 1740

gactttaaac ttaatgaaga gatcgccatt attttggcat ctttttctgc ttccacaagt 1800

gcttttgtgg aaactgtgaa aggtttggat tataaagcat tcaaacagat tgttgaatcc 1860

tgtggtaatt ttaaggttac aaagggaaaa gctaaaaaag gtgcctggaa tattggtgaa 1920

cagaaatcaa tactgagtcc tctttatgca tttgcatcag aggctgctcg tgttgtacga 1980

tcaattttct cccgcactct tgaaactgct caaaattctg tgcgtgtttt acagaaggcc 2040

gctataacaa tactagatgg aatttcacag tattcactga gactcattga tgctatgatg 2100

ttcacatctg atttggctac taacaatcta gttgtaatgg cctacattac aggtggtgtt 2160

gttcagttga cttcgcagtg gctaactaac atctttggca ctgtttatga aaaactcaaa 2220

cccgtccttg attggcttga agagaagttt aaggaaggtg tagagtttct tagagacggt 2280

tgggagattg ttaaattcat ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc 2340

acctgtgcta aggaaattaa ggagagtgtt cagacattct ttaagcttgt aaacaagttt 2400

ttggctttgt gtgctgactc tatcattatt ggtggagcta aacttaaagc cttgaattta 2460

ggtgaaacat ttgtcacgca ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa 2520

gaaactggcc tactcatgcc tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa 2580

acacttccca cagaagtgtt aacagaggaa gttgtcttga aaactggtga tttacaacca 2640

ttagaacaac ctactagtga agctgttgaa gctccattgg ttggtacacc agtttgtatt 2700

aacgggctta tgttgctcga aatcaaagac acagaaaagt actgtgccct tgcacctaat 2760

atgatggtaa caaacaatac cttcacactc aaaggcggtg caccaacaaa ggttactttt 2820

ggtgatgaca ctgtgataga agtgcaaggt tacaagagtg tgaatatcac ttttgaactt 2880

gatgaaagga ttgataaagt acttaatgag aagtgctctg cctatacagt tgaactcggt 2940

acagaagtaa atgagttcgc ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca 3000

gtatctgaat tacttacacc actgggcatt gatttagatg agtggagtat ggctacatac 3060

tacttatttg atgagtctgg tgagtttaaa ttggcttcac atatgtattg ttctttctac 3120

cctccagatg aggatgaaga agaaggtgat tgtgaagaag aagagtttga gccatcaact 3180

caatatgagt atggtactga agatgattac caaggtaaac ctttggaatt tggtgccact 3240

tctgctgctt tacaacctga agaagaacaa gaagaagatt ggttagatga tgatagtcaa 3300

caaactgttg gtcaacaaga cggcagtgag gacaatcaga caactactat tcaaacaatt 3360

gttgaggttc aacctcaatt agagatggaa cttacaccag ttgttcagac tattgaagtg 3420

aatagtttta gtggttatct taaacttact gacaatgtat acatcaagaa tgcagacatt 3480

gtggaagaag ctaaaaaggt aaaaccaaca gtggttgtta atgcagccaa tgtttacctt 3540

aaacatggag gaggtgttgc aggagcctta aataaggcta ctaacaatgc catgcaagtt 3600

gaatctgatg attacatagc tactaatgga ccacttaaag tgggtggtag ttgtgtttta 3660

agcggacaca atcttgctaa acactgttta catgttgtcg gcccaaatgt taacaaaggt 3720

gaagatattc aacttcttaa gagtgcttat gaaaatttta accagcacga agttctactt 3780

gcaccattat tatcagctgg tatttttggt gctgacccta tacattcttt aagagtttgt 3840

gtagatactg ttcgcacaaa tgtctactta gctgtctttg ataaaaatct ctatgacaaa 3900

cttgtttcaa gctttttgga aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag 3960

attcctaaag aggaagttaa gccatttata actgaaagta aaccttcagt tgaacagaga 4020

aaacaagatg ataagaagat caaagcttgt gttgaagaag ttacaacaac tctggaagaa 4080

actaagttcc tcacagaaaa cttgctcctt tatatcgaca ttaatggcaa tcttcatcca 4140

gattctgcca ctcttgttag tgacattgac atcactttct taaagaaaga tgctccatat 4200

atagtgggtg atgttgttca agagggtgtt ttaactgctg tggttatacc tactaaaaag 4260

gctggtggca ctactgaaat gctagcgaaa gctttgagaa aagtgccaac agacaattat 4320

ataaccactt acccgggtca gggtttaaat ggttacactg tagaggaggc aaagacagtg 4380

cttaaaaagt gtaaaagtgc cttttacatt ctaccatcta ttatctctaa tgagaagcaa 4440

gaaattcttg gaactgtttc ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca 4500

cgcaaattaa tgcctgtctg tgtggaaact aaagccatag tttcaactat acagcgtaaa 4560

tataagggta tcaagataca agagggtgtg gttgattatg gtgctagatt ttacttttac 4620

accagtaaaa caactgtagc gtcacttatc aacacactta acgatctaaa tgaaactctt 4680

gttacaatgc cacttggcta tgtaacacat ggcttaaatt tggaagaagc tgctcggtat 4740

atgagatctc tcaaagtgcc agctacagtt tctgtttctt cacctgatgc tgttacagcg 4800

tataatggtt atcttacttc ttcttctaaa acacctgaag aacattttat tgaaaccatc 4860

tcacttgctg gttcctataa agattggtcc tattctggac aatctacaca actaggtata 4920

gaatttctta agagaggtga taaaagtgta tattacacgt ccaatcctac cacattccac 4980

ctagatggtg aagttatcac ctttgacaat cttaagacac ttctttcttt gagagaagtg 5040

aggactatta aggtgtttac aacagtagac aacattaacc tccacacgca agttgtggac 5100

atgtcaatga catatggaca acagtttggt ccaacttatt tggatggagc tgatgttact 5160

aagataaaac ctcataactc acatgaaggt aaaacatttt acgttttgcc taatgatgac 5220

actctacgtg ttgaggcttt tgagtactac cacacaactg atcctagttt tctgggtagg 5280

tacatgtcag cattaaatca cactaaaaag tggaaatacc cacaagttaa tggtttaact 5340

tcgattaaat gggcagataa caactgttat cttgccactg cattgttaac actccaacaa 5400

atagagttga agtttaatcc acctgctcta caagatgctt attacagagc aagggctggt 5460

gaagctgcta acttttgtgc acttatctta gcctactgta ataagacagt aggtgagtta 5520

ggtgatgtta gagaaacaat gagttacttg tttcaacatg ccaatttaga ttcttgcaaa 5580

agagtcttga acgtggtgtg taaaacttgt ggacaacagc agacaaccct taagggtgta 5640

gaagctgtta tgtacatggg cacactttct tatgaacaat tcaagaaagg tgttcagata 5700

ccttgtacgt gtggtaaaca agctacaaaa tatctagtac aacaggagtc accttttgtt 5760

atgatgtcag caccacctgc tcagtatgaa cttaagcatg gtacatttac ttgtgctagt 5820

gagtacactg gtaattacca gtgtggtcac tataagcata taacttctaa ggaaactttg 5880

tattgcatag acggtgcttt acttacaaag tcctcagaat acaaaggtcc tattacggat 5940

gttttctaca aagaaaacag ttacacaaca accataaaac cagttactta taagttggat 6000

ggtgttgttt gtacagaaat tgaccctaag ttggacaatt attataagaa ggacaactct 6060

tatttcacag agcaaccaat tgatcttgta ccaaaccaac catatccaaa cgcaagcttc 6120

gataatttta agttcgtatg cgataatatc aaatttgctg atgatctcaa ccagttaact 6180

ggttataaga aacctgcttc aagagagctt aaagttacat ttttccctga cttaaatggt 6240

gatgtggtgg ctattgatta taaacactac acaccctctt ttaagaaagg agctaaattg 6300

ttacataagc ctattgtttg gcatgttaac aatgcaacta ataaagccac gtataaacca 6360

aatacctggt gtatacgttg tctttggagc acaaaaccag ttgaaacatc aaattcgttt 6420

gatgtactga agtcagagga cgcgcaggga atggataatc ttgcatgtga agatctaaaa 6480

ccagtctctg aagaagtagt ggaaaatcct accatacaga aagacgttct tgagtgtaat 6540

gtgaaaacta ccgaagttgt aggagacatt atacttaaac cagcaaataa tagtttgaag 6600

atcacagaag aggttggcca cacagatcta atggctgctt atgtagacaa ttctagtctt 6660

actattaaga aacctaatga actctctaga gtattaggtt tgaaaaccct tgctactcat 6720

ggtttagctg ctgttaatag tgtcccttgg gatactatag ctaattatgc taagcctttt 6780

cttaacaaag ttgttagtac aactactaac atagttacac ggtgtcttaa tcgtgtttgt 6840

actaattata tgccttactt ctttacttta ttgctacaat tgtgtacttt tactagaagt 6900

acaaattcta gaatcaaggc atctatgccg actactatag caaagaatac tgttaagagt 6960

gtcggtaaat tttgtctaga ggcttcattt aattatctca agtcacctaa cttttctaag 7020

ctgataaaca ttatcatctg gtttttgcta ttaagtgttt gcctaggttc tttaatctac 7080

tcaaccgctg ctttaggtgt tttaatgtct aatttaggca tgccttctta ctgtactggt 7140

tacagagaag gctatttgaa ctctactaat gtcactattg caacctactg tactggatct 7200

ataccttgta gtgtttgtct tagtggttta gattctttag acacctatcc ttctcttgaa 7260

actatacaga ttaccatttc atctttcaaa tgggatttaa ctgcttttgg cttagttgca 7320

gagtggtttt tggcatatat tcttttcact aggtttttct atgtacttgg attggctgca 7380

atcatgcaat tgtttttcag ctattttgca gtccatttta ttagtaactc ttggcttatg 7440

tggcttataa ttaatcttgt gcagatggcc ccgatttcag ctatggttag aatgtacatc 7500

ttctttgcct cattttatta tgtgtggaaa agttatgtgc atgttgtaga cggttgtaat 7560

tcatcaactt gtatgatgtg ttacaaacgt aatagagcaa caagagtcga atgtacaact 7620

attgttaatg gtgttagaag gtccttttat gtctatgcta atggaggtaa aggcttttgc 7680

aaactacaca attggaattg tgttaattgt gatacattct gtgctggtag tacatttatt 7740

agtgatgaag ttgcgagaga cttgtcacta cagtttaaaa gaccaataaa tcctactgac 7800

caatcttctt acatcgttga tagtgttaca gtgaagaatg gttccatcca tctttacttt 7860

gataaagctg gtcaaaagac ttatgaaaga cattctctct ctcattttgt taacttagac 7920

aacctgagag ctaataacac taaaggttca ttgcctatta atgttatcgt tttcgacggt 7980

aaatcaaaat gtgaagaatc atctgcaaaa tcagcgtctg tttactacag tcagcttatg 8040

tgtcaaccta tactgttact agatcaggca ttagtgtctg atgttggtga tagtgcggaa 8100

gttgcagtta aaatgtttga tgcttacgtt aatacgtttt catcaacttt taacgtacca 8160

atggaaaaac tcaaaacact agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc 8220

ttagacaatg tcttatctac gtttatttca gcagctcggc aagggtttgt tgattcagat 8280

gtagaaacta aagatgttgt tgaatgtctt aaattgtcac atcaatctga catagaagtt 8340

actggcgata gttgtaataa ctatatgctc acctataaca aagttgaaaa catgacaccc 8400

cgtgaccttg gtgcttgtat tgactgtagt gctagacata ttaatgcgca ggtagcaaaa 8460

agtcacaaca ttgctttgat atggaacgtt aaagatttca tgtcattgtc tgaacaacta 8520

cgaaaacaaa tacgtagtgc tgctaaaaag aataacttac ccttcaagtt gacatgtgca 8580

actactagac aagttgttaa tgttgtaaca acaaagatag cacttaaggg tggtaaaatt 8640

gtgaataact ggttgaagca gcttattaaa gttacacttg tgttcctttt tgttgctgct 8700

attttctatc tgataacacc tgttcatgtc atgtctaaac atactgactt ttcaagtgaa 8760

atcataggat acaaggctat tgatggtggt gtcactcgtg acatagcatc tacagatact 8820

tgttttgcta acaaacatgc tgattttgac acatggttta gccagcgtgg tggtagttat 8880

actaatgaca aagcttgccc attgattgct gcagtcataa caagagaagt gggttttgtc 8940

gttcctggtt tgcctggaac gatattacgc acaactaatg gtgacttttt gcatttctta 9000

cctagagttt ttagtgcagt tggtaacatc tgttacacac catcaaaact tatagagtac 9060

actgactttg caacatcagc ttgtgttttg gctgctgaat gtacaatttt taaagacgct 9120

tctggtaagc cagtaccata ttgttatgat accaatgtac tagaaggttc tgttgcttat 9180

gaaagtttac gccctgacac acgttatgtg ctcatggatg gctctattat tcaatttcct 9240

aacacctacc ttgaaggttc tgtaagagtg gtaacaactt ttgattctga gtactgtagg 9300

cacggcactt gtgaaagatc agaagctggt gtttgtgtat ctactagtgg tagatgggta 9360

cttaacaacg attattacag atctttacca ggagttttct gtggtgtaga tgctgtaaat 9420

ttgcttacta acatgtttac accactaatt caacctattg gtgctttgga catatcagca 9480

tctatagtag ctggtggtat tgtagctatc gtagtaacat gccttgccta ctattttatg 9540

aggtttagac gtgcttttgg tgaatacagt catgtagttg cctttaatac tctcctattc 9600

cttatgtcat tcactgtact ctgtttaaca ccagtttact cattcttacc tggtgtttat 9660

tctgttattt acctgtactt gacattttat ctgactaatg atgtttcttt tctcgcacat 9720

attcagtgga tggttatgtt cacaccttta gtacctttct ggataacaat tgcttacatc 9780

atttgtattt ccacaaagca tttctattgg ttctttagta attacctaaa gagacgtgta 9840

gtctttaatg gtgtttcctt tagtactttt gaagaagctg cgctgtgcac ctttttgtta 9900

aataaggaga tgtatctaaa gttgcgtagt gatgtgctat tacctcttac gcaatataat 9960

agatacttag ctctttataa caagtacaag tatttcagtg gagcaatgga tacaactagc 10020

tacagagaag ctgcttgttg tcatctcgca aaggctctca atgacttcag taactcaggt 10080

tctgatgttc tttaccaacc accacaaacc tctatcacct cagctgtttt gcagagtggt 10140

tttagaaaaa tggcattccc atctggtaaa gttgagggtt gtatggtaca agtaacttgt 10200

ggtacaacta cacttaacgg tctttggctt gatgacgtag tttactgtcc aagacatgtg 10260

atctgcacct ctgaagatat gcttaaccct aattatgaag atctactcat ccgtaagtct 10320

aatcataact tcttggtaca ggctggtaat gttcaactca gggttattgg acattctatg 10380

caaaattgtg tacttaagct taaggttgat acagccaatc ctaagacacc taagtataag 10440

tttgttcgca ttcaaccagg acagactttt tcagtgttag cttgttacaa tggttcacca 10500

tctggtgttt accaatgtgc tatgaggccc aatttcacta ttaagggttc attccttaat 10560

ggttcatgtg gtagtgttgg ttttaacata gattatgact gtgtctcttt ttgttacatg 10620

caccatatgg aattaccaac tggagttcat gctggcacag acttagaagg taacttttat 10680

ggaccttttg ttgacaggca aacagcacaa gcagctggta cagatacaac tattacagtt 10740

aatgttcttg cttggttgta cgctgctgtt ataaatggag acaggtggtt tctcaatcga 10800

tttaccacaa ctcttaatga ctttaacctt gtggctatga agtacaatta tgaacctcta 10860

acacaagacc atgttgacat actaggacct ctttctgctc aaactggaat tgccgtttta 10920

gatatgtgtg cttcattaaa agaacttctg caaaatggta tgaatggacg taccatattg 10980

ggtagtgctt tattagaaga tgagtttaca ccttttgatg ttgttagaca atgctcaggt 11040

gttactttcc aaagtgcagt gaaaagaaca atcaagggta cacaccactg gttgttactc 11100

acaattttga cttcactttt agttttagtc cagagtactc aatggtcttt gttctttttc 11160

ttctacgaaa atgccttttt accttttgct atgggtatta ttgctatgtc tgcttttgca 11220

atgatgtttg tcaaacataa gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc 11280

actgtagctt actttaatat ggtctacatg cctgctagtt gggtgatgcg tattatgaca 11340

tggttggata tggttgatac tagtttgtct ggttttaagc taaaagactg tgttatgtat 11400

gcatcagctg tagtgttact aatccttatg acagcaagaa ctgtgtatga tgatggtgct 11460

aggagagtgt ggacacttat gaatgtcttg acactcgttt ataaagttta ctatggcaac 11520

gctttagatc aagccatttc catgtgggct cttataatct ctgttacttc taactactca 11580

ggtgtagtta caactgtcat gtttttggcc agaggtattg tttttatgtg tgttgagtat 11640

tgccctattt tcttcataac tggtaataca cttcagtgta taatgctagt ctattgtttc 11700

ttaggctatt tttgtacttg ttacttcggc ctcttttgtt tactcaaccg ctactttaga 11760

ctgactcttg gtgtttatga ttacttagtg tctacacagg agtttagata tatgaattca 11820

cagggactac tcccacccaa gaatagcata gatgccttca aactcaacat taaattgttg 11880

ggtgttggtg gcaaaccttg tatcaaagta gccactgtac agtctaaaat gtcagatgta 11940

aagtgcacat cagtagtctt actctcagtt ttgcaacaac tcagagtaga atcatcatct 12000

aaattgtggg ctcaatgtgt ccagttacac aatgacattc tcttagctaa agatactact 12060

gaagcctttg aaaaaatggt ttcactactt tctgttttgc tttccatgca gggtgctgta 12120

gacataaaca agctttgtga agaaatgctg gacaacaggg caaccttaca agctatagcc 12180

tcagagttta gttcccttcc atcatatgca gcttttgcta ctgctcaaga agcttatgag 12240

caggctgttg ctaatggtga ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat 12300

gtggctaaat ctgaatttga ccgtgatgca gccatgcaac gtaagttgga aaagatggct 12360

gatcaagcta tgacccaaat gtataaacag gctagatctg aggacaagag ggcaaaagtt 12420

actagtgcta tgcagacaat gcttttcact atgcttagaa agttggataa tgatgcactc 12480

aacaacatta tcaacaatgc aagagatggt tgtgttccct tgaacataat acctcttaca 12540

acagcagcca aactaatggt tgtcatacca gactacaaca catataagaa tacgtgtgat 12600

ggtacaacat ttacttatgc atcagcattg tgggaaatcc aacaggttgt agatgcagat 12660

agtaaaattg ttcagcttag tgaaattagt atggacaatt cacctaattt agcatggcct 12720

cttattgtaa cagctttaag ggccaattct gctgtcaaat tacagaataa tgagcttagt 12780

cctgttgcac taagacaaat gtcttgtgct gccggtacta cacaaactgc ttgcactgat 12840

gacaatgcgt tagcttacta caacacaaca aagggaggta ggtttgtact tgcactgtta 12900

tccgatttac aggatttgaa atgggctaga ttccctaaga gtgatggaac tggtactatc 12960

tatacagaac tggaaccacc ttgtaggttt gttacagaca cacctaaagg tcctaaagtg 13020

aagtatcttt acttcatcaa aggattaaac aacctaaata gaggtatggt acttggtagt 13080

ttagctgcca cagtacgttt acaagctggt aatgcaacag aagttcctgc taattcaact 13140

gtactttctt tctgtgcttt tgctgtagat gctgctaaag cttacaaaga ttatctagct 13200

agtgggggac aaccaatcac taattgtgtt aagatgttgt gtacacacac tggtactggt 13260

caggcaataa cagttacacc ggaagccaat atggatcaag aatcctttgg tggtgcatcg 13320

tgttgtctgt actgccgttg tcatatagat catccaaatc ctaaaggatt ttgtgactta 13380

aaaggtaagt atgtacaaat acctacaact tgtgctaatg accctgtggg ttttacactt 13440

aaaaacacag tctgtaccgt ctgcggtatg tggaaaggtt atggttgtag ttgtgatcaa 13500

ctccgcgaac ccatgcttca gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg 13560

taagtgcagc ccgtcttaca ccgtgcggca caggcactag tactgatgtc gtatatagag 13620

cttttgacat ctacaatgat aaagtagctg gttttgctaa gttcctaaaa actaattgtt 13680

gtcgcttcca agaaaaggac gaagatgaca atctcattga ttcttacttt gtagttaaga 13740

gacacacttt ctctaactac caacatgaag aaacaattta caacctgctt aaggattgtc 13800

cagctgttgc taaacatgac ttctttaagt ttagaataga cggtgacatg gtaccacata 13860

tatcacgtca acgtcttact aaatacacaa tggcagacct cgtctatgct ttaaggcatt 13920

ttgatgaagg taattgtgac acattaaaag aaatacttgt cacatacaat tgttgtgatg 13980

atgactactt caataaaaag gactggtatg attttgtaga aaacccagat atattacgcg 14040

tatacgccaa cttaggtgaa cgtgtacgcc aagctttgtt aaaaacagta cagttctgtg 14100

atgccatgcg aaatgctggt attgttggtg tactgacatt agataatcaa gatctcaatg 14160

gtaactggta tgactttggt gatttcatac aaaccacgcc aggtagtgga gttcctgttg 14220

tagactctta ttattcattg ctcatgccta tattaacctt gaccagggct ttaactgcag 14280

agtcacatgt tgacactgac ttaacaaagc cttacattaa gtgggatttg ttaaaatacg 14340

acttcacgga agagaggtta aaactctttg accgttattt taaatactgg gatcagacat 14400

accacccaaa ttgtgttaac tgtttggatg acagatgcat tctgcattgt gcaaacttta 14460

atgttctgtt ctctacagtg ttcccaccta caagttttgg accactagtg agaaaaatat 14520

ttgttgatgg tgttccattt gtagtttcaa ctggatacca cttcagagag ctaggtgttg 14580

tacataatca ggatgtaaac ttacatagct ctagacttag ttttaaggaa ttacttgtgt 14640

atgctgctga tcctgctatg catgctgctt ctggtaatct attactagat aaacgcacta 14700

cgtgcttttc agtagctgca cttactaaca atgttgcttt tcaaactgtc aaacccggta 14760

attttaacaa ggacttctat gactttgctg tgtctaaggg tttctttaag gaaggaagtt 14820

ctgttgaatt aaaacacttc ttctttgctc aggatggtaa tgctgctatc agcgattatg 14880

actactatcg ttataatcta ccaacaatgt gtgatatcag acaactacta tttgtagttg 14940

aagttgttga taagtacttt gattgttacg atggtggctg tattaatgct aaccaagtca 15000

tcgtcaacaa cctagacaaa tcagctggtt ttccatttaa taaatggggt aaggctagac 15060

tttattatga ttccatgagt tatgaggatc aagatgcact tttcgcatat acaaaacgta 15120

atgtcatccc tactataact caaatgaacc ttaagtatgc cattagtgca aagaatagag 15180

ctcgcaccgt agctggtgtc tctatctgta gtactatgac caatagacag tttcatcaaa 15240

aattactcaa gtcaatagcc gccactagag gagctactgt agtaattgga acaagcaaat 15300

tctatggtgg ttggcacaac atgctcaaaa ctgtttatag tgatgtagaa aaccctcacc 15360

ttatgggttg ggattatcct aaatgtgata gagccatgcc taacatgctt agaattatgg 15420

cctcacttgt tcttgctcgc aaacatacaa cgtgttgtag cttgtcacac cgtttctata 15480

gattagctaa tgagtgtgct caagtattga gtgaaatggt catgtgtggc ggttcactat 15540

atgttaaacc aggtggaacc tcatcaggag atgccacaac tgcttatgct aatagtgtgt 15600

ttaacatttg tcaagctgtc acggccaatg ttaatgcact tttatctact gatggtaaca 15660

aaattgccga taagtatgtc cgcaatttac aacacagact ttatgagtgt ctctatagaa 15720

atagagatgt tgacacagac tttgtgaatg agttttacgc atatttgcgt aaacatttct 15780

caatgatgat actctctgac gatgctgttg tgtgtttcaa tagcacttat gcatctcaag 15840

gtctagtggc tagcataaag aactttaagt cagttcttta ctatcaaaac aacgttttta 15900

tgtctgaagc aaaatgttgg actgagactg accttactaa aggacctcat gaattttgct 15960

ctcaacatac aatgctagtt aaacagggtg atgattatgt gtaccttcct tacccagatc 16020

catcaagaat cctaggtgcc ggttgttttg tagatgatat cgtaaaaaca gatggtacac 16080

ttatgattga acggttcgtg tctttagcta tagatgctta cccacttact aaacatccta 16140

atcaggagta tgctgatgtc tttcatttgt acttacaata catacgtaag ctacatgatg 16200

agttaacagg acacatgtta gacatgtatt ctgttatgct tactaatgat aacacttcaa 16260

ggtattggga acctgagttt tatgaggcta tgtacacacc gcatacagtc ttacaagctg 16320

ttggtgcttg tgttctttgc aattcacaga cttcattaag atgtggtgct tgcatacgta 16380

gaccattctt atgttgtaaa tgctgttacg accatgtcat ctcaacatca cataaattag 16440

tcttgtctgt taatccgtat gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc 16500

aactttactt aggaggtatg agctattact gtaagtcaca taaaccaccc attagttttc 16560

cattgtgtgc taatggacaa gtttttggtc tctacaagaa tacatgtgtt ggtagcgata 16620

atgttactga ctttaatgca attgcaacat gtgactggac aaatgctggt gattacattt 16680

tagctaacac ctgtactgaa agactcaagc tttttgcagc agaaacgctc aaagctactg 16740

aggagacatt taaactgtct tatggtattg ctactgtacg tgaagtgctg tctgacagag 16800

aattacatct ttcatgggaa gttggtaaac ctagaccacc acttaaccga aattatgtct 16860

ttactggtta tcgtgtaact aaaaacagta aagtgcaaat cggagagtac acctttgaaa 16920

aaggtgacta tggtgatgct gttgtttacc gaggtacaac aacttacaaa ctcaacgttg 16980

gtgattattt tgtgctgaca tcacatacag taatgccatt aagtgcacct acactagtgc 17040

cacaagagca ctatgttaga attactggct tatacccaac actcaatatc tcagatgagt 17100

tttctagcaa tgttgcaaat tatcaaaagg ttggtatgca aaagtattct acactccagg 17160

gaccacctgg tactggtaaa agtcattttg ctattggtct agctctctac tacccttctg 17220

ctcgcatagt atatacagct tgctctcatg cagctgttga tgcactatgt gagaaggcat 17280

taaaatattt gcccatagac aaatgtagta gaattatacc tgcacgtgct cgtgtagagt 17340

gttttgataa attcaaggtg aattcaacat tagaacagta tgtcttttgt actgtaaatg 17400

cattgcctga gacgacagca gatatagttg tctttgatga aatttcaatg gccacaaatt 17460

atgatttgag tgttgtcaat gccagattac gtgctaagca ctatgtgtac attggtgatc 17520

ctgctcaatt acctgcacca cgcacattac taactaaggg tacactagaa ccagaatatt 17580

tcaattcagt gtgtagactt atgaaaacta taggtccaga catgttcctc ggaacttgtc 17640

gtagatgtcc tgctgaaatt gttgacactg tgagtgcttt ggtttatgat aataagctta 17700

aggcacataa agacaaatca gctcaatgct ttaaaatgtt ctacaagggt gttatcacgc 17760

atgatgtttc atctgcaatt aacaggccac aaataggcgt ggtaagagaa ttccttacac 17820

gtaaccctgc ttggagaaaa gctgtcttta tttcacctta caattcccag aatgctgtag 17880

cctcaaagat tttgggacta ccaactcaaa ctgttgattc atcacagggc tcagaatatg 17940

actatgtcat attcactcaa accactgaaa cagctcactc ttgtaatgta aacagattca 18000

acgttgctat taccagagca aaagtaggca tactttgcat aatgtctgat agagaccttt 18060

atgacaagtt gcaatttaca agtcttgaaa ttccacgtag gaatgtggca actttacaag 18120

ctgaaaatgt aacaggactc tttaaagatt gtagtaaggt aatcactggg ttacatccta 18180

cacaggcacc tacacactta agtgttgata ctaaattcaa aactgaaggt ttatgtgttg 18240

acatacctgg catacctaag gacatgacct atagaagatt aatctctatg atgggtttca 18300

aaatgaatta ccaggttaat ggttacccta acatgtttat cacccgcgaa gaagctataa 18360

gacatgtacg tgcatggatt ggcttcgatg tcgaaggttg tcatgctact agagaagctg 18420

ttggtaccaa tttaccttta cagctaggtt tttctacagg tgttaaccta gttgctgtac 18480

ctacaggtta tgttgataca cctaataata cagatttttc cagagttagt gctaaaccac 18540

cgcctggaga tcaatttaaa cacctcatac cacttatgta caaaggactt ccttggaatg 18600

tagtgcgtat aaagattgtc caaatgttaa gtgacacact taaaaatctc tctgacagag 18660

tcgtatttgt cttatgggca catggctttg agttgacatc tatgaagtat tttgtgaaga 18720

tcggacctga gcgcacatgt tgtctatgtg atagacgtgc tacatgcttt tccactgctt 18780

cagacactta tgcctgttgg catcattcta ttggatttga ttacgtctat aatccgttta 18840

tgattgatgt tcaacaatgg ggttttacag gtaacctaca aagcaaccat gatctgtatt 18900

gtcaagtcca tggtaatgca catgtagcta gttgtgatgc aatcatgact aggtgtctag 18960

ctgtccacga gtgctttgtt aagcgtgttg actggactat tgaatatcct ataatcggtg 19020

atgaactgaa gattaatgcg gcttgtagaa aggttcaaca catggttgtt aaagctgcat 19080

tattagcaga caaattccca gttcttcacg acattggtaa ccctaaagct attaagtgtg 19140

tacctcaagc tgatgtagaa tggaagttct atgatgcaca gccttgtagt gacaaagctt 19200

acaaaataga agaactgttc tattcttatg ccacacattc tgacaaattc acagatggtg 19260

tatgcctatt ttggaattgc aatgtcgata gatatcctgc taattccatt gtttgtagat 19320

ttgacactag agtgctatct aaccttaact tgcctggttg tgatggtggc agtttgtatg 19380

taaataagca tgcattccac acaccagctt ttgataaaag tgcttttgtt aatctaaagc 19440

aacttccatt tttctattac tctgacagtc catgtgagtc tcatggaaaa caagtagtgt 19500

cagatataga ttatgtacca ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg 19560

gtgctgtctg tagacatcat gctaatgagt acagattgta tctcgatgct tataacatga 19620

tgatctcagc tggctttagc ttgtgggttt acaaacaatt tgatacctat aacctctgga 19680

acacttttac aagacttcag agtttagaaa atgtggcttt taatgttgta aataagggac 19740

actttgatgg acaacagggt gaagtaccag tttctatcat taacaacact gtttacacaa 19800

aagttgatgg tgttgatgta gaattgtttg agaacaaaac cacattacct gttaatgtag 19860

catttgagct ttgggctaag cgcaacatta aaccagtacc agaggtgaaa atactcaata 19920

atttgggtgt ggacattgct gctaatactg tgatctggga ctacaaaaga gatgctccag 19980

cacatatatc tactattggt gtttgttcta tgactgacat agccaagaaa ccaactgaaa 20040

cgatttgtgc accactcact gtcttttttg atggtagagt tgatggtcaa gtagacttat 20100

ttagaaatgc ccgtaatggt gttcttatta cagaaggtag tgttaaaggt ttacaaccat 20160

ctgtaggtcc caaacaagct agtcttaatg gagtcacatt aattggagaa gccgtaaaaa 20220

cacagttcaa ttattacaag aaagtggatg gtgttgtcca acaattacct gaaacttact 20280

ttactcagag tagaaactta caggaattta agcccaggag tcaaatggaa attgatttct 20340

tagaacttgc tatggatgaa ttcattgaac ggtataaatt agaaggctat gccttcgaac 20400

atatcgttta tggagatttt agtcatagtc agttaggtgg tttacatcta ctgattggac 20460

tagctaaacg ttttaaggaa tcaccttttg aacttgaaga ttttattcct atggacagta 20520

cagttaaaaa ctacttcata acagatgcgc aaacaggttc atctaagtgt gtgtgttctg 20580

ttattgatct tttacttgat gacttcgttg aaataataaa gtcccaagat ttatctgtag 20640

tttctaaggt tgtcaaagtg actattgact atacagaaat ctcatttatg ctttggtgta 20700

aagatggcca tgtagaaaca ttttacccaa aattacaatc tagtcaagcg tggcaaccgg 20760

gtgttgctat gcctaatctt tacaaaatgc aaagaatgct attagaaaag tgtgaccttc 20820

aaaattatgg tgatagtgca acattaccta aaggcataat gatgaatgtc gcaaaatata 20880

ctcaactgtg tcaatattta aacacactga cattagctgt accctataat atgagagtta 20940

tccattttgg tgctggttct gataaaggag ttgcaccagg tacagctgtt ttaagacaat 21000

ggttgcctac aggtacgctg cttgtcgatt cagatcttaa tgactttgtc tctgatgcag 21060

attcaacttt gattggtgat tgtgcaactg tacatacagc taataaatgg gatctcatta 21120

ttagtgatat gtacgaccct aagactaaga atgtcacaaa agaaaacgac tctaaagagg 21180

gttttttcac ttacatttgt gggtttatac aacaaaagct agctcttgga ggttccgtgg 21240

ctataaagat aacagaacat tcttggaatg ctgatcttta taagctcatg ggacacttcg 21300

catggtggac agcctttgtt actaatgtga atgcgtcatc atctgaagca tttttaatcg 21360

gatgtaacta ccttggcaaa ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt 21420

acatattttg gaggaataca aatccaattc agctttcttc ttattcttta ttcgacatga 21480

gtaaattccc ccttaaatta aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca 21540

atgatatgat tctctctctt cttagtaaag gtagacttat aattagagaa aacaacagag 21600

ttgttatttc tagtgatgtt cttgttaaca actaaacgaa caatgtttgt ttttcttgtt 21660

ttattgccac tagtctctag tcagtgtgtt aatcttacaa ccagaactca attaccccct 21720

gcatacacta attctttcac acgtggtgtt tattaccctg acaaagtttt cagatcctca 21780

gttttacatt caactcagga cttgttctta cctttctttt ccaatgttac ttggttccat 21840

gctatacatg tctctgggac caatggtact aagaggtttg ataaccctgt cctaccattt 21900

aatgatggtg tttactttgc ttccactgag aagtctaaca taataagagg ctggattttt 21960

ggtactactt tagattcgaa aacccagtcc ctacttattg ttaataacgc tactaatgtt 22020

gttatcaaag tctgtgaatt tcaattttgt aacgatccat ttttgggtgt ttattaccac 22080

aaaaacaaca aaagttggat ggaaagtgag ttcagagttt attctagtgc gaataattgc 22140

acttttgaat acgtctctca gccttttctt atggaccttg aaggaaaaca gggtaatttc 22200

aaaaatctta gggaatttgt gttcaagaat attgatggtt acttcaagat atactctaag 22260

cacacgccta ttaatttagt gcgtgatctc cctcagggtt tttcggcttt agaaccattg 22320

gtagatttgc caataggtat taacatcact aggtttcaaa ctttacttgc tttacataga 22380

agttatttaa ctcctggtga ttcttcttca ggttggacag ctggtgctgc agcttattat 22440

gtgggttatc ttcaacctag gacttttcta ctgaagtaca atgaaaatgg aaccattaca 22500

gatgctgtag actgtgcact tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc 22560

actgtagaaa aaggaatcta tcaaacttct aactttagag tccaaccaac agaatctatt 22620

gttagatttc ctaacatcac aaacttgtgc ccttttggtg aagtttttaa cgccaccaga 22680

tttgcatctg tttatgcttg gaacaggaag agaatcagca actgtgttgc tgattattct 22740

gtcctgtata attccgcatc attttccact tttaagtgtt atggagtgtc tcctactaaa 22800

ttaaatgatc tctgctttac taatgtctat gcagattcat ttgtaattag aggtgatgaa 22860

gtcagacaaa tcgctccagg gcaaactgga aagattgctg attataacta caaattacca 22920

gatgatttta caggctgcgt tatagcttgg aattctaaca atcttgattc taaggttggt 22980

ggtaattata attacctgta cagattgttt aggaagtcta atctcaaacc ttttgagaga 23040

gatatttcaa ctgaaatcta tcaggccggt agcacacctt gtaatggtgt tgaaggtttt 23100

aattgttact ttcctctgca atcatatggt ttccaaccca ctaatggtgt tggttaccaa 23160

ccatacagag tagtagtact ttcttttgaa cttctacatg caccagcaac tgtttgtgga 23220

cctaaaaagt ctactaattt ggttaagaac aagtgtgtca atttcaactt caatggttta 23280

acaggcacag gtgttcttac tgagtctaac aaaaagtttc tgcctttcca acaatttggc 23340

agagacattg ctgacactac tgatgctgtt cgtgatccac aaacacttga gattcttgac 23400

attacaccat gttcttttgg tggtgtcagt gttataacac caggaacaaa tacttctaac 23460

caggttgctg ttctttatca ggatgttaac tgcacagaag tccctgttgc tattcatgca 23520

gatcaactta ctcctacttg gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt 23580

gcaggctgtt taataggggc tgaacatgtc aacaactcat atgagtgtga catacccatt 23640

ggtgcaggta tatgcgctag ttatcagact cagactaatt ctcctcggag agcaagaagt 23700

gtagctagtc aatccatcat tgcctacact atgtcacttg gtgcagaaaa ttcagttgct 23760

tactctaata actctattgc catacccaca aattttacta ttagcgttac cacagaaatt 23820

ctaccagtgt ctatgaccaa gacatcagta gattgtacaa tgtacatttg tggtgattca 23880

actgaatgca gcaatctttt gttgcaatat ggcagttttt gtacacaatt aaaccgtgct 23940

ttaactggaa tagctgttga acaagacaaa aacacccaag aagtttttgc acaagtcaaa 24000

caaatttaca agacaccacc aattaaagat tttggcggtt ttaattttag ccagatactg 24060

ccagatccat caaaaccaag caagaggtca tttattgaag atctactgtt caacaaagtg 24120

acacttgcag atgctggctt catcaaacaa tatggtgatt gccttggtga tattgctgct 24180

agagacctca tttgtgcaca aaagtttaac ggccttactg ttttgccacc tttgctcaca 24240

gatgaaatga ttgctcaata cacttctgca ctgttagcag gtacaatcac ttctggttgg 24300

acttttggtg caggtgctgc attacaaata ccatttgcta tgcaaatggc ttataggttt 24360

aatggtattg gagttacaca gaatgttctc tatgagaacc aaaaattgat tgccaaccaa 24420

tttaatagtg ctattggcaa aattcaagac tcactttctt ccacagcaag tgcacttgga 24480

aaacttcaag atgtggtcaa ccaaaatgca caagctttaa acacgcttgt taaacaactt 24540

agctccaatt ttggtgcaat ttcaagtgtt ttaaacgaca tcctttcacg tcttgacaaa 24600

gttgaggctg aagtgcaaat tgataggttg atcacaggca gacttcaaag tttgcagaca 24660

tatgtgactc aacaattaat tagagctgca gaaatcagag cttctgctaa tcttgctgct 24720

actaaaatgt cagagtgtgt acttggacaa tcaaaaagag ttgacttttg cggaaagggc 24780

tatcatctta tgtcatttcc tcagtcagca cctcatggtg tcgtcttttt gcatgtgact 24840

tatgtccctg cacaagaaaa gaacttcaca actgctcctg ccatttgtca tgatggaaaa 24900

gcacactttc ctcgtgaagg tgtctttgtt tcaaatggca cacactggtt tgtaacacaa 24960

aggaattttt atgaaccaca aatcattact acagacaaca catttgtgtc tggtaactgt 25020

gatgttgtaa taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac 25080

tcattcaagg aggagcttga taaatacttc aagaaccata cctcaccaga tgttgattta 25140

ggtgacatct ctggcattaa tgcttcagtt gtaaacattc agaaagaaat cgaccgcctc 25200

aatgaggttg ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat 25260

gagcagtata taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc 25320

atagtaatgg tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc 25380

tgttgttctt gtggatcctg ctgcaaattt gacgaggacg actctgagcc agtgctcaaa 25440

ggagtcaaat tacattacac ataaacgaac ttatggattt gtttatgaga atcttcacaa 25500

ttggaactgt aactttgaag caaggtgaaa tcaaggatgc tactccttca gattttgtta 25560

gagctactgc aacgataccg atacaagcat cacttccttt cggatggctt attgttggcg 25620

ttgcacttct tgctgttttt cagagcgctt ccaaaatcat aaccctcaaa aagagatggc 25680

aactagcact ctccaagggt gttcactttg tttgcaactt gctgttgttg tttgtaacag 25740

tttactcaca tcttttgctt gttgctgctg gccttgaagc cccttttctc tatctttatg 25800

ctttagtcta cttcttgcag agtataaact ttgtacgcat aataatgagg ctttggcttt 25860

gctggaaatg ccgttccaaa aacccattac tttatgatgc caactatttt ctttgctggc 25920

atactaattg ttacgactat tgtatacctt acaatagtgt aacttcttca attgtcatta 25980

cttcaggtga tggcacaaca agtcctattt ctgaacatga ctaccagatt ggtggttata 26040

ctgaaaaatg ggaatctgga gtaaaagact gtgttgtatt acacagttac ttcacttcag 26100

actattacca gctgtactca actcaattga gtacagacac tggtgttgaa catgttacct 26160

tcttcatcta caataaaatc gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg 26220

acgtttcatc cggagttgtt aatccagtaa tggaaccaat ttatgatgaa ccgacgacga 26280

ctactagcgt gcctttgtaa gcacaagctg atgagtacga acttatgtac tcattcgttt 26340

cggaagagac aggtacgtta atagttaata gcgtacttct ttttcttgct ttcgtggtat 26400

tcttgctagt tacactagcc attcttactg cgcttcgatt gtgtgcgtac tgttgcaata 26460

ttgttaacgt gagtcttgta aaaccttctt tttacgttta ctctcgtgtt aaaaatctga 26520

attcttctcg ggttcctgat cttctggtct aaacgaacta aatattatat tagtttttct 26580

gtttggaact ttaattttag ccatggcaga ttccaacggt actattaccg ttgaggagct 26640

gaaaaagctc cttgaacaat ggaacctagt aataggtttc ctattcctta catggatttg 26700

cctgctgcaa tttgcctatg ccaacaggaa taggtttttg tacatcatta agttgatttt 26760

cctctggctg ttatggccag taactttagc ttgttttgtg cttgctgctg tttacagaat 26820

aaattggatc accggtggaa ttgctattgc aatggcttgt cttgtaggat tgatgtggct 26880

aagctacttc attgcttctt tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa 26940

tccagaaact aacattcttc tcaacgtgcc actccatgga actattctga ctagaccgct 27000

tctagaaagt gaactcgtaa tcggagctgt tatccttcgt ggacatcttc gtattgctgg 27060

acatcatcta ggacgctgtg acatcaagga tctacctaaa gaaatcactg ttgctacatc 27120

acgaacgctt tcttattaca aattgggagc ttcacagcgt gtagcaggtg attcaggttt 27180

tgctgcatat agtcgctaca ggattggcaa ctataaatta aacacagacc attccagtag 27240

cagtgacaat attgctttgc ttgtacagta agtgacaaca gatgtttcat ctcgttgact 27300

ttcaggttac tatagcagag atattactaa tcatcatgag gacttttaaa gtttccattt 27360

ggaatcttga ttacatcata aacctcataa ttaagaactt aagcaagtca ctaactgaga 27420

ataaatattc tcaactagac gaggagcagc caatggagat tgattaaacg aacatgaaaa 27480

ttattctttt cttggcactg ataacactcg ctacttgtga gctttatcac taccaagagt 27540

gtgttagagg tacaacagta cttttaaaag aaccttgctc gtcgggaaca tacgagggca 27600

attcaccatt tcatcctcta gctgataaca aatttgcact gacttgcttt agcactcaat 27660

ttgcttttgc ttgtcctgac ggcgtaaaac acgtctatca gttacgtgcc agatcagttt 27720

cacctaaact gttcatcaga caagaggaag ttcaagaact ttactctcca atttttctta 27780

ttgttgcggc aatagtgttt ataacacttt gcttcacact caaaagaaag acagaatgat 27840

tgaactttca ttaattgact tctatttgtg ctttttagcc tttctgctat tccttgtttt 27900

aattatgctt attatctttt ggttctcact tgaactgcaa gatcataatg aaacttgtca 27960

cgcctaaacg aacatgaaat ttcttgtttt cttaggaatc atcacaactg tagctgcatt 28020

tcaccaagaa tgtagtttac agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc 28080

gtgtcctatt cacttctatt ctaaatggta tatcagagta ggagctagaa aatcagcacc 28140

tttaattgaa ttgtgcgtgg atgaggctgg ttctaaatca cccattcagt acatcgatat 28200

cggtaattat acagtttcct gtttaccttt tacaattaac tgccaggaac ctaaattggg 28260

tagtcttgta gtgcgttgtt cgttctacga ggacttttta gagtatcatg acgttcgtgt 28320

tgttttagat ttcatctaaa cgaacaaact aaaatgtctg ataatggacc tcaaaatcag 28380

cgaaatgcac ctcgcattac gtttggtgga ccatcagatt caactggcag taaccagaat 28440

ggagaacgaa gtggtgcgcg atcaaaacaa cgccgcccgc aaggtttacc caataatact 28500

gcgtcttggt tcaccgctct cactcaacat ggcaaggaag atttaaaatt ccctcgagga 28560

caaggcgttc caattaacac caatagcagt ccagatgacc aaattggcta ctaccgccgc 28620

gccacaagac gaattcgtgg tggtgatggt aaaatgaaag atctcagtcc aagatggtat 28680

ttctactatc taggaactgg gccagaagct ggacttcctt atggtgctaa caaagatggc 28740

atcatatggg ttgcaactga gggagccttg aatacaccaa aagatcacat tggcaccaga 28800

aatcctgcta acaatgctgc aatcgtgcta caacttcctc aaggaacaac attaccaaaa 28860

ggtttttacg cagaagggtc tagaggtgga agtcaagcct cttctagatc atcatcacgt 28920

agtcgcaaca gttcaagaaa ttcaactcca ggttcaagta gaggaacttc tcctgctaga 28980

atggctggaa atggaggtga tgctgctctt gctttgttac tacttgacag attgaaccag 29040

cttgagagca aaatgtctgg taaaggccaa caacaacaag gccaaactgt cactaagaaa 29100

tctgctgctg aggcttctaa gaagcctaga caaaaacgta ctgccactaa agcatacaat 29160

gtaacacaag ctttcggcag acgtggtcca gaacaaactc aaggaaattt tggggatcag 29220

gaactaatca gacaaggaac tgattacaaa cattggccgc aaattgcaca atttgctcct 29280

tctgcttcag cgttctttgg aatgtcgaga attggaatgg aagtcacacc ttcgggaaca 29340

tggttgacct atacaggtgc catcaaattg gatgacaaag atccaaattt caaagatcaa 29400

gtcattttgc tgaataagca tattgacgca tacaaaacat tcccaccaac agagcctaaa 29460

aaggacaaaa agaagaaggc tgatgaaact caagccttac cgcagagaca gaagaaacag 29520

caaactgtga ctcttcttcc tgctgcagat ttggatgatt tctccaaaca attgcaacaa 29580

tccatgagca gtgctgactc aactcaggcc taaactcatg cagaccacac aaggcagatg 29640

ggctatataa acgttttcgc ttttccgttt acgatatata gtctactctt gtgcagaatg 29700

aattctcgta actacatagc acaagtagat gtagttaact ttaatctcac atagcaatct 29760

ttaatcagtg tgtaacatta gggaggactt gaaagagcca ccacattttc accgaggcca 29820

cgcggagtac gatcgagtgt acagtgaaca atgctaggga gagctgccta tatggaagag 29880

ccctaatgtg taaaattaat tttagtagtg ctatccccat gtgattttaa tagcttctta 29940

ggagaatgac aaaaaaaaac aaaaaaaa 29968

<210> 45

<211> 10827

<212> DNA

<213> Artificial sequence

<220>

<223> vector

<400> 45

cggccgtaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 60

gctttatttg tgaaatttgt gatgctatag ctttatttgt aaccattata agctgcaata 120

aacaagttgt ttaaaccacg tgatgaccat acacctcggg atactagatg tataatgtcc 180

gccatgcaga cgaaaccagt cggagattac cgagcattct atcacgtcgg cgaccaatag 240

tgagcttagg gataacaggg taataaacga tccccgggaa ttcactggcc gtcgttttac 300

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 360

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 420

gcagcctgaa tggcgaatgg cgatagatcc ggtggatgac cttttgaatg acctttaata 480

gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt aaaaattttt 540

tcacaaaacg gtttacaagc ataaagctcg gacggatctt ttccgctgca taaccctgct 600

tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat 660

tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga 720

taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc 780

tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag 840

atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca 900

aggtgtcgat gcaggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat 960

tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 1020

tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa 1080

ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 1140

aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 1200

tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 1260

ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 1320

actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 1380

aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 1440

ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 1500

gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 1560

acaagtctgg aaagaaatgc ataagttttt gccattctca ccggattcag tcgtcactca 1620

tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 1680

tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 1740

cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 1800

tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 1860

taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga 1920

ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac 1980

cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc 2040

atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag 2100

tcagcaacac cttcttcacg aggcagacct cagacggtat cggatcgatc ccccgatgtg 2160

tagcagtggc ggaccatata ggcagatcag aaggcgcggt tctcctacat gagcttttca 2220

attcaattca tcattttttt tttattcttt tttttgattt cggtttcctt gaaatttttt 2280

tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt 2340

atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact 2400

gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga 2460

acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa 2520

gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt 2580

tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt 2640

ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact 2700

cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg 2760

tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg 2820

tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt 2880

gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac 2940

tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat 3000

gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga 3060

caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc 3120

tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg 3180

tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta 3240

aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta 3300

attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa 3360

aaaaattgga aagaaaaagc tgggcgcgcc ggccggccct tttcatcacg tgctataaaa 3420

ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa 3480

gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc 3540

caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt 3600

catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg 3660

ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt 3720

acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttttcttc attccgtaac 3780

tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac 3840

acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag 3900

gcaagcgatc ggccggcccg ggcatttaaa tgcaggccgc gtacgcgtcg acggtaccga 3960

attcgcttaa acgagctcat gttcgccggt gaacgcgttg aggaagccgg gcagtgcctc 4020

ggcaaaatcc ttgcgtgtag acaagacatc tgcgtagcag ttgtcctcaa caacgatgtc 4080

gaaatccaaa tcggagtgct catcgagtcc tccgtgaacg taagagccgc cgatcagaag 4140

agcgcggaag cgaacatcgg aagcgaccgc atcgcggatg cggttcaaga aagttgcatg 4200

agcttgtgga agtgtgctga gcataaatga ttctcctagc tgttctttgg gtaagtacgc 4260

catcaggacg ttgtgagtgg cgcgattttt agcggctgaa atcagccctt gagcctgtcg 4320

gcaagtcgcg tcatgaggtc catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg 4380

cccgcacgcg gcaggcaaaa aacgtagtgg tgttcgcagc gggccatccg cagcgcggga 4440

aagagttcgc tcatgtcctt aaacgggcct tcgccggcgg caagcctggc tatgccctgt 4500

tccagcttag cgatatagcg gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg 4560

atgatgccgc gtagatcggc ttcggcctca gccgtgagga tgtaggccgt caagcgcgat 4620

ccccgctgag ttcttcatca agaatttcgc cgacgctctt ggtggacacc ttgccggcaa 4680

gcccatcgtt gatgcggttc cccagcatgg ttttcagttc ctgccatgcc tgatcggcat 4740

cagcgtcacc ggggaacaga cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg 4800

cggccagggc tttcaggctc tggtgctgct ggtccgtcat gtcgattgtc aggcggctca 4860

ttggataacc tccataaaat acacgtaacc acattagcac atatgtgggc gtgaggctac 4920

agcgcgaggc gcattaaggt cgggaaaatg cgctaggcgc atttaaattg cgtattgctg 4980

taatgcgcca tgccggctag actaggccca aatgggtata cccaatttga ccaaggggga 5040

cgcgatgagg gcggccaagc actaccgaca acttctatcc atcgacttca acatcgaggc 5100

gctggccttc gtgcctggac ccgacggcac acgcggccgg cgcatccacg tcctggggcg 5160

cgaggtccgc gaccggcccg gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt 5220

ggcgctggac ggctactgca aggccaattt cgatgcagtg ctgcacctgg cgtaccccga 5280

tcatcagcaa tggggccacg catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc 5340

gcgctggccg tcctggtcgt tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg 5400

cgcatcatcg acggcgacac catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc 5460

ctggtggaca ttgacgcgcc ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg 5520

ctggccggca tggtgttccg ccggcacgtc ctggtcgacg agaaggacac cgaccgttac 5580

ggccgcacgc tgggcaccgt gtgggtcaac atggagctgg ccagccggcc gccgcagccg 5640

cgcaacgtca acgccgcgat ggttcaccag ggcatggcgt gggcctatcg cttccacggc 5700

cgcgcggccg accctgaaat gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc 5760

ctctggtccg atccgcacgc cgtcgagccg tggaaatggc gacgcgagag caacaaccgg 5820

agggacgaag gttgaaggtc gcccgcatct acctgcgcgc cagtacggac gagcagaatc 5880

ttgaacgcca ggagagcctt gtagcggcca cgcgggccgc cgggtactac gtcgccggca 5940

tctaccgcga gaaggcgtcc ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg 6000

cggacctgca acctggtgaa gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc 6060

cgttggccga ggccgagcgc ctggttgcgt cgatccgggc caaaggggcc aagctggccg 6120

tgcctggcgt ggtggacctg tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg 6180

ttctggaatc cgtccaggac atgcttttga agctcgcctt gcagatggcc cgcgacgact 6240

acgaggatcg gcgcgagcgt caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct 6300

acaccggccg caaacgtgac gccggcatgc acgaccgcat catcacgctt cgctccggcg 6360

gatcgagcat tgccaagacg gccaagctgg tcggatgcag cccgagccag gtcaaacgag 6420

tgtgggcggc ctggaacgcg cagcagcaaa aataaagccg ggcagtgccc ggcttttctc 6480

accttttcgc gtcccgcagg gccgctgcga gcgccctacc tagatcctcg ctttccccct 6540

cggtgtagtc cggccagggc acgaagggcg cggatgcgaa cctgttgagc aggtacgcct 6600

tcgggcagcg gtagaccacc ggcgagttcg ccttttcatc ccaccgggcc aggatcacgt 6660

ccgcatcaca gtgcatgtcc ttcacctggt cgcggaagaa gccgaaggcc accatgccgc 6720

tatgttcgcc gaggaacgcc agttgcttcg cgctggcgat cgcgccgacg ccgccggcca 6780

aaaccgacgc catcacccag ccgacgaacc agaagctggc atgcttgcgg ttgaccaccg 6840

cacgcgcagc cgcgaccagg acaacggcca agctgccgac cagggccatg acgaccgtga 6900

tccggccgtt gtggaaagcg atgggcttgc cagcgtccgc ttgcacggcg tcgtaaatgc 6960

tggacccgat gggcgcgcac atcagcacga caggcagcag caccaggaac atcgtccgcg 7020

tccattgcgc gagtgccttg cggcgttcgc cggcggcaag cgcctccatc atcggcgtga 7080

agcccaacag ggccaccgca gccgccaagc cggcaacgat gccgcaggcg attacataca 7140

tacatcctcc ctaatgcgcc ttgcgcacgg ttgtagtcag agtccgcggt ggggcgataa 7200

gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 7260

aaagatcaaa ggatcttctt gagatccttt ttttctgcgg gggatcagga ccgctgccgg 7320

agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc ctttccaccg 7380

cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta gcgtccaagc 7440

ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcctgctgcg gcgtccgctc 7500

gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc tggcgctcgc gggcgaggtc 7560

cagggcggcc gtcttcacgt tctgccttgc gcagatgaga tagatcgatc tagcgtggac 7620

tcaaggctct cgcgaatggc tcgcgttgga aactttcatt gacacttgag gggcaccgca 7680

gggaaattct cgtccttgcg agaaccggct atgtcgtgct gcgcatcgag cctgcgccct 7740

tggcttgtct cgcccctctc cgcgtcgcta cggggcttcc agcgcctttc cgacgctcac 7800

cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga 7860

aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga 7920

aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc 7980

cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc 8040

agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac 8100

aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg 8160

aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt 8220

gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt 8280

tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt 8340

ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc 8400

ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct 8460

cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga 8520

tcggggcagt aacgggatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc 8580

cgcaggtgct ggcatcgaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg 8640

gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg 8700

caatttttac cttgggcatt cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg 8760

gtgaattaat tccccggatc gatccgtcag cttcacgctg ccgcaagcac tcagggcgca 8820

agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc 8880

ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc 8940

aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa 9000

gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa 9060

actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tcgacggatc 9120

gatccgggga attaattccg gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga 9180

ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac 9240

catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat 9300

ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc cccctgccct 9360

gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg cggcaggttt 9420

ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc gaaaaaccgc 9480

cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc tgaaacacac 9540

gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt ggccggacac 9600

gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc gcaacaagaa 9660

aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg acgtgaagat 9720

cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt 9780

ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct acgagctttg 9840

ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg aatgcctgtc 9900

gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc tggaatcggt 9960

gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt 10020

cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg 10080

ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt tcagctcgca 10140

ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat cggattccac 10200

ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg 10260

cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct agggccttgt 10320

ggggtcagtt ccggctgggg gttcagcagc cactcgatcg aggtcccaat acgcaaaccg 10380

cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 10440

aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 10500

gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 10560

cacacaggaa acagctatga ccatgattac gccaagcttc catgggatat cgagatctcc 10620

tgcagagctc tagagtcgag actagtctcg acgggcccgg taccccctcg agggggccgc 10680

acttaagtta cgcgtggatc gtggagcttt cgggttttaa ctataacggt cctaaggtag 10740

cgaactcggg tcttgcctta atcccaacaa ccggattatc tacacggatt tcaatagctg 10800

atatagcgaa tcaccgagat taattaa 10827

<210> 46

<211> 506

<212> DNA

<213> Artificial sequence

<220>

<223> origin of replication

<400> 46

atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa 60

atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag 120

actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat 180

taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt 240

tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata 300

aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt 360

ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac 420

ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg 480

cgcgtgtaag ttacaggcaa gcgatc 506

<210> 47

<211> 1020

<212> DNA

<213> Artificial sequence

<220>

<223> selection marker

<400> 47

ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt 60

gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta 120

tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg 180

cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa 240

cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag 300

caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt 360

gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt 420

tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 480

ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 540

gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 600

attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg 660

atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact 720

gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 780

ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 840

aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 900

gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 960

gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 1020

<210> 48

<211> 228

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 E

<400> 48

atgtactcat tcgtttcgga agagacaggt acgttaatag ttaatagcgt acttcttttt 60

cttgctttcg tggtattctt gctagttaca ctagccattc ttactgcgct tcgattgtgt 120

gcgtactgtt gcaatattgt taacgtgagt cttgtaaaac cttcttttta cgtttactct 180

cgtgttaaaa atctgaattc ttctcgggtt cctgatcttc tggtctaa 228

<210> 49

<211> 669

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 M

<400> 49

atggcagatt ccaacggtac tattaccgtt gaggagctga aaaagctcct tgaacaatgg 60

aacctagtaa taggtttcct attccttaca tggatttgcc tgctgcaatt tgcctatgcc 120

aacaggaata ggtttttgta catcattaag ttgattttcc tctggctgtt atggccagta 180

actttagctt gttttgtgct tgctgctgtt tacagaataa attggatcac cggtggaatt 240

gctattgcaa tggcttgtct tgtaggattg atgtggctaa gctacttcat tgcttctttc 300

agactgtttg cgcgtacgcg ttccatgtgg tcattcaatc cagaaactaa cattcttctc 360

aacgtgccac tccatggaac tattctgact agaccgcttc tagaaagtga actcgtaatc 420

ggagctgtta tccttcgtgg acatcttcgt attgctggac atcatctagg acgctgtgac 480

atcaaggatc tacctaaaga aatcactgtt gctacatcac gaacgctttc ttattacaaa 540

ttgggagctt cacagcgtgt agcaggtgat tcaggttttg ctgcatatag tcgctacagg 600

attggcaact ataaattaaa cacagaccat tccagtagca gtgacaatat tgctttgctt 660

gtacagtaa 669

<210> 50

<211> 1260

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 N

<400> 50

atgtctgata atggacctca aaatcagcga aatgcacctc gcattacgtt tggtggacca 60

tcagattcaa ctggcagtaa ccagaatgga gaacgaagtg gtgcgcgatc aaaacaacgc 120

cgcccgcaag gtttacccaa taatactgcg tcttggttca ccgctctcac tcaacatggc 180

aaggaagatt taaaattccc tcgaggacaa ggcgttccaa ttaacaccaa tagcagtcca 240

gatgaccaaa ttggctacta ccgccgcgcc acaagacgaa ttcgtggtgg tgatggtaaa 300

atgaaagatc tcagtccaag atggtatttc tactatctag gaactgggcc agaagctgga 360

cttccttatg gtgctaacaa agatggcatc atatgggttg caactgaggg agccttgaat 420

acaccaaaag atcacattgg caccagaaat cctgctaaca atgctgcaat cgtgctacaa 480

cttcctcaag gaacaacatt accaaaaggt ttttacgcag aagggtctag aggtggaagt 540

caagcctctt ctagatcatc atcacgtagt cgcaacagtt caagaaattc aactccaggt 600

tcaagtagag gaacttctcc tgctagaatg gctggaaatg gaggtgatgc tgctcttgct 660

ttgttactac ttgacagatt gaaccagctt gagagcaaaa tgtctggtaa aggccaacaa 720

caacaaggcc aaactgtcac taagaaatct gctgctgagg cttctaagaa gcctagacaa 780

aaacgtactg ccactaaagc atacaatgta acacaagctt tcggcagacg tggtccagaa 840

caaactcaag gaaattttgg ggatcaggaa ctaatcagac aaggaactga ttacaaacat 900

tggccgcaaa ttgcacaatt tgctccttct gcttcagcgt tctttggaat gtcgagaatt 960

ggaatggaag tcacaccttc gggaacatgg ttgacctata caggtgccat caaattggat 1020

gacaaagatc caaatttcaa agatcaagtc attttgctga ataagcatat tgacgcatac 1080

aaaacattcc caccaacaga gcctaaaaag gacaaaaaga agaaggctga tgaaactcaa 1140

gccttaccgc agagacagaa gaaacagcaa actgtgactc ttcttcctgc tgcagatttg 1200

gatgatttct ccaaacaatt gcaacaatcc atgagcagtg ctgactcaac tcaggcctaa 1260

<210> 51

<211> 21290

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 ORF1ab

<400> 51

atggagagcc ttgtccctgg tttcaacgag aaaacacacg tccaactcag tttgcctgtt 60

ttacaggttc gcgacgtgtt agtacgtggt tttggagatt cagtggaaga agtcttatca 120

gaggcacgtc aacatcttaa agatggcact tgtggcttag tagaagttga aaaaggcgtt 180

ttgcctcaac ttgaacagcc ctatgtgttc atcaaacgtt ctgatgctag aactgcacct 240

catggtcatg ttatggttga gctggtagca gaattagaag gtattcagta cggtcgtagt 300

ggtgagacat taggtgtttt agttcctcat gtgggcgaaa taccagtggc ttaccgcaaa 360

gttcttctta gaaagaacgg taataaagga gctggtggcc atagttacgg cgctgattta 420

aagtcatttg acttaggcga cgagcttggc actgatcctt atgaagattt ccaagaaaac 480

tggaacacta aacatagcag tggtgttacc cgtgaactca tgcgtgagtt aaatggaggt 540

gcatacactc gctatgtcga taacaacttc tgtggacctg atggttaccc tcttgagtgc 600

attaaagacc ttctagcacg tgctggtaaa gcttcatgca ctttgtccga acaactggac 660

tttattgaca ctaagagggg tgtatactgc tgccgtgaac atgagcatga aattgcttgg 720

tacacggaac gttctgaaaa gagctatgaa ttgcagacac cttttgaaat taaactggca 780

aagaaatttg acaccttcaa tggggaatgt ccaaattttg tatttcccct caattccata 840

atcaagacta ttcaaccaag ggttgaaaag aaaaagcttg atggctttat gggtagaatt 900

cgatctgtct atccagttgc gtcaccaaat gaatgcaacc aaatgtgcct ttcaactctc 960

atgaagtgtg atcattgtgg tgaaacttca tggcagacgg gcgattttgt taaagccact 1020

tgcgaatttt gtggcactga gaatttgact aaagaaggtg ccactacttg tggttactta 1080

ccccaaaatg ctgttgttaa aatttactgt ccagcatgtc acaattcaga agtaggacct 1140

gagcatagtc ttgccgaata ccataatgaa tctggcttga aaaccattct tcgtaagggt 1200

ggtcgcacta ttgcttttgg aggatgtgtg ttctcttatg ttggttgcca taacaagtgt 1260

gcttattggg ttccacgtgc ttcagctaac ataggttgta accatacagg tgttgttgga 1320

gaaggttccg aaggtcttaa tgacaacctt cttgaaatac tccaaaaaga gaaagtcaac 1380

atcaatattg ttggtgactt taaacttaat gaagagatcg ccattatttt ggcatctttt 1440

tctgcttcca caagtgcttt tgtggaaact gtgaaaggtt tggattataa agcattcaaa 1500

cagattgttg aatcctgtgg taattttaag gttacaaagg gaaaagctaa aaaaggtgcc 1560

tggaatattg gtgaacagaa atcaatactg agtcctcttt atgcatttgc atcagaggct 1620

gctcgtgttg tacgatcaat tttctcccgc actcttgaaa ctgctcaaaa ttctgtgcgt 1680

gttttacaga aggccgctat aacaatacta gatggaattt cacagtattc actgagactc 1740

attgatgcta tgatgttcac atctgatttg gctactaaca atctagttgt aatggcctac 1800

attacaggtg gtgttgttca gttgacttcg cagtggctaa ctaacatctt tggcactgtt 1860

tatgaaaaac tcaaacccgt ccttgattgg cttgaagaga agtttaagga aggtgtagag 1920

tttcttagag acggttggga gattgttaaa ttcatctcaa cctgtgcttg tgaaattgtc 1980

ggtggacaaa ttgtcacctg tgctaaggaa attaaggaga gtgttcagac attctttaag 2040

cttgtaaaca agtttttggc tttgtgtgct gactctatca ttattggtgg agctaaactt 2100

aaagccttga atttaggtga aacatttgtc acgcactcaa agggattgta cagaaagtgt 2160

gttaaatcca gagaagaaac tggcctactc atgcctctaa aagccccaaa agaaattatc 2220

ttcttagagg gagaaacact tcccacagaa gtgttaacag aggaagttgt cttgaaaact 2280

ggtgatttac aaccattaga acaacctact agtgaagctg ttgaagctcc attggttggt 2340

acaccagttt gtattaacgg gcttatgttg ctcgaaatca aagacacaga aaagtactgt 2400

gcccttgcac ctaatatgat ggtaacaaac aataccttca cactcaaagg cggtgcacca 2460

acaaaggtta cttttggtga tgacactgtg atagaagtgc aaggttacaa gagtgtgaat 2520

atcacttttg aacttgatga aaggattgat aaagtactta atgagaagtg ctctgcctat 2580

acagttgaac tcggtacaga agtaaatgag ttcgcctgtg ttgtggcaga tgctgtcata 2640

aaaactttgc aaccagtatc tgaattactt acaccactgg gcattgattt agatgagtgg 2700

agtatggcta catactactt atttgatgag tctggtgagt ttaaattggc ttcacatatg 2760

tattgttctt tctaccctcc agatgaggat gaagaagaag gtgattgtga agaagaagag 2820

tttgagccat caactcaata tgagtatggt actgaagatg attaccaagg taaacctttg 2880

gaatttggtg ccacttctgc tgctttacaa cctgaagaag aacaagaaga agattggtta 2940

gatgatgata gtcaacaaac tgttggtcaa caagacggca gtgaggacaa tcagacaact 3000

actattcaaa caattgttga ggttcaacct caattagaga tggaacttac accagttgtt 3060

cagactattg aagtgaatag ttttagtggt tatcttaaac ttactgacaa tgtatacatc 3120

aagaatgcag acattgtgga agaagctaaa aaggtaaaac caacagtggt tgttaatgca 3180

gccaatgttt accttaaaca tggaggaggt gttgcaggag ccttaaataa ggctactaac 3240

aatgccatgc aagttgaatc tgatgattac atagctacta atggaccact taaagtgggt 3300

ggtagttgtg ttttaagcgg acacaatctt gctaaacact gtttacatgt tgtcggccca 3360

aatgttaaca aaggtgaaga tattcaactt cttaagagtg cttatgaaaa ttttaaccag 3420

cacgaagttc tacttgcacc attattatca gctggtattt ttggtgctga ccctatacat 3480

tctttaagag tttgtgtaga tactgttcgc acaaatgtct acttagctgt ctttgataaa 3540

aatctctatg acaaacttgt ttcaagcttt ttggaaatga agagtgaaaa gcaagttgaa 3600

caaaagatcg ctgagattcc taaagaggaa gttaagccat ttataactga aagtaaacct 3660

tcagttgaac agagaaaaca agatgataag aagatcaaag cttgtgttga agaagttaca 3720

acaactctgg aagaaactaa gttcctcaca gaaaacttgc tcctttatat cgacattaat 3780

ggcaatcttc atccagattc tgccactctt gttagtgaca ttgacatcac tttcttaaag 3840

aaagatgctc catatatagt gggtgatgtt gttcaagagg gtgttttaac tgctgtggtt 3900

atacctacta aaaaggctgg tggcactact gaaatgctag cgaaagcttt gagaaaagtg 3960

ccaacagaca attatataac cacttacccg ggtcagggtt taaatggtta cactgtagag 4020

gaggcaaaga cagtgcttaa aaagtgtaaa agtgcctttt acattctacc atctattatc 4080

tctaatgaga agcaagaaat tcttggaact gtttcttgga atttgcgaga aatgcttgca 4140

catgcagaag aaacacgcaa attaatgcct gtctgtgtgg aaactaaagc catagtttca 4200

actatacagc gtaaatataa gggtatcaag atacaagagg gtgtggttga ttatggtgct 4260

agattttact tttacaccag taaaacaact gtagcgtcac ttatcaacac acttaacgat 4320

ctaaatgaaa ctcttgttac aatgccactt ggctatgtaa cacatggctt aaatttggaa 4380

gaagctgctc ggtatatgag atctctcaaa gtgccagcta cagtttctgt ttcttcacct 4440

gatgctgtta cagcgtataa tggttatctt acttcttctt ctaaaacacc tgaagaacat 4500

tttattgaaa ccatctcact tgctggttcc tataaagatt ggtcctattc tggacaatct 4560

acacaactag gtatagaatt tcttaagaga ggtgataaaa gtgtatatta cacgtccaat 4620

cctaccacat tccacctaga tggtgaagtt atcacctttg acaatcttaa gacacttctt 4680

tctttgagag aagtgaggac tattaaggtg tttacaacag tagacaacat taacctccac 4740

acgcaagttg tggacatgtc aatgacatat ggacaacagt ttggtccaac ttatttggat 4800

ggagctgatg ttactaagat aaaacctcat aactcacatg aaggtaaaac attttacgtt 4860

ttgcctaatg atgacactct acgtgttgag gcttttgagt actaccacac aactgatcct 4920

agttttctgg gtaggtacat gtcagcatta aatcacacta aaaagtggaa atacccacaa 4980

gttaatggtt taacttcgat taaatgggca gataacaact gttatcttgc cactgcattg 5040

ttaacactcc aacaaataga gttgaagttt aatccacctg ctctacaaga tgcttattac 5100

agagcaaggg ctggtgaagc tgctaacttt tgtgcactta tcttagccta ctgtaataag 5160

acagtaggtg agttaggtga tgttagagaa acaatgagtt acttgtttca acatgccaat 5220

ttagattctt gcaaaagagt cttgaacgtg gtgtgtaaaa cttgtggaca acagcagaca 5280

acccttaagg gtgtagaagc tgttatgtac atgggcacac tttcttatga acaattcaag 5340

aaaggtgttc agataccttg tacgtgtggt aaacaagcta caaaatatct agtacaacag 5400

gagtcacctt ttgttatgat gtcagcacca cctgctcagt atgaacttaa gcatggtaca 5460

tttacttgtg ctagtgagta cactggtaat taccagtgtg gtcactataa gcatataact 5520

tctaaggaaa ctttgtattg catagacggt gctttactta caaagtcctc agaatacaaa 5580

ggtcctatta cggatgtttt ctacaaagaa aacagttaca caacaaccat aaaaccagtt 5640

acttataagt tggatggtgt tgtttgtaca gaaattgacc ctaagttgga caattattat 5700

aagaaggaca actcttattt cacagagcaa ccaattgatc ttgtaccaaa ccaaccatat 5760

ccaaacgcaa gcttcgataa ttttaagttc gtatgcgata atatcaaatt tgctgatgat 5820

ctcaaccagt taactggtta taagaaacct gcttcaagag agcttaaagt tacatttttc 5880

cctgacttaa atggtgatgt ggtggctatt gattataaac actacacacc ctcttttaag 5940

aaaggagcta aattgttaca taagcctatt gtttggcatg ttaacaatgc aactaataaa 6000

gccacgtata aaccaaatac ctggtgtata cgttgtcttt ggagcacaaa accagttgaa 6060

acatcaaatt cgtttgatgt actgaagtca gaggacgcgc agggaatgga taatcttgca 6120

tgtgaagatc taaaaccagt ctctgaagaa gtagtggaaa atcctaccat acagaaagac 6180

gttcttgagt gtaatgtgaa aactaccgaa gttgtaggag acattatact taaaccagca 6240

aataatagtt tgaagatcac agaagaggtt ggccacacag atctaatggc tgcttatgta 6300

gacaattcta gtcttactat taagaaacct aatgaactct ctagagtatt aggtttgaaa 6360

acccttgcta ctcatggttt agctgctgtt aatagtgtcc cttgggatac tatagctaat 6420

tatgctaagc cttttcttaa caaagttgtt agtacaacta ctaacatagt tacacggtgt 6480

cttaatcgtg tttgtactaa ttatatgcct tacttcttta ctttattgct acaattgtgt 6540

acttttacta gaagtacaaa ttctagaatc aaggcatcta tgccgactac tatagcaaag 6600

aatactgtta agagtgtcgg taaattttgt ctagaggctt catttaatta tctcaagtca 6660

cctaactttt ctaagctgat aaacattatc atctggtttt tgctattaag tgtttgccta 6720

ggttctttaa tctactcaac cgctgcttta ggtgttttaa tgtctaattt aggcatgcct 6780

tcttactgta ctggttacag agaaggctat ttgaactcta ctaatgtcac tattgcaacc 6840

tactgtactg gatctatacc ttgtagtgtt tgtcttagtg gtttagattc tttagacacc 6900

tatccttctc ttgaaactat acagattacc atttcatctt tcaaatggga tttaactgct 6960

tttggcttag ttgcagagtg gtttttggca tatattcttt tcactaggtt tttctatgta 7020

cttggattgg ctgcaatcat gcaattgttt ttcagctatt ttgcagtcca ttttattagt 7080

aactcttggc ttatgtggct tataattaat cttgtgcaga tggccccgat ttcagctatg 7140

gttagaatgt acatcttctt tgcctcattt tattatgtgt ggaaaagtta tgtgcatgtt 7200

gtagacggtt gtaattcatc aacttgtatg atgtgttaca aacgtaatag agcaacaaga 7260

gtcgaatgta caactattgt taatggtgtt agaaggtcct tttatgtcta tgctaatgga 7320

ggtaaaggct tttgcaaact acacaattgg aattgtgtta attgtgatac attctgtgct 7380

ggtagtacat ttattagtga tgaagttgcg agagacttgt cactacagtt taaaagacca 7440

ataaatccta ctgaccaatc ttcttacatc gttgatagtg ttacagtgaa gaatggttcc 7500

atccatcttt actttgataa agctggtcaa aagacttatg aaagacattc tctctctcat 7560

tttgttaact tagacaacct gagagctaat aacactaaag gttcattgcc tattaatgtt 7620

atcgttttcg acggtaaatc aaaatgtgaa gaatcatctg caaaatcagc gtctgtttac 7680

tacagtcagc ttatgtgtca acctatactg ttactagatc aggcattagt gtctgatgtt 7740

ggtgatagtg cggaagttgc agttaaaatg tttgatgctt acgttaatac gttttcatca 7800

acttttaacg taccaatgga aaaactcaaa acactagttg caactgcaga agctgaactt 7860

gcaaagaatg tgtccttaga caatgtctta tctacgttta tttcagcagc tcggcaaggg 7920

tttgttgatt cagatgtaga aactaaagat gttgttgaat gtcttaaatt gtcacatcaa 7980

tctgacatag aagttactgg cgatagttgt aataactata tgctcaccta taacaaagtt 8040

gaaaacatga caccccgtga ccttggtgct tgtattgact gtagtgctag acatattaat 8100

gcgcaggtag caaaaagtca caacattgct ttgatatgga acgttaaaga tttcatgtca 8160

ttgtctgaac aactacgaaa acaaatacgt agtgctgcta aaaagaataa cttacccttc 8220

aagttgacat gtgcaactac tagacaagtt gttaatgttg taacaacaaa gatagcactt 8280

aagggtggta aaattgtgaa taactggttg aagcagctta ttaaagttac acttgtgttc 8340

ctttttgttg ctgctatttt ctatctgata acacctgttc atgtcatgtc taaacatact 8400

gacttttcaa gtgaaatcat aggatacaag gctattgatg gtggtgtcac tcgtgacata 8460

gcatctacag atacttgttt tgctaacaaa catgctgatt ttgacacatg gtttagccag 8520

cgtggtggta gttatactaa tgacaaagct tgcccattga ttgctgcagt cataacaaga 8580

gaagtgggtt ttgtcgttcc tggtttgcct ggaacgatat tacgcacaac taatggtgac 8640

tttttgcatt tcttacctag agtttttagt gcagttggta acatctgtta cacaccatca 8700

aaacttatag agtacactga ctttgcaaca tcagcttgtg ttttggctgc tgaatgtaca 8760

atttttaaag acgcttctgg taagccagta ccatattgtt atgataccaa tgtactagaa 8820

ggttctgttg cttatgaaag tttacgccct gacacacgtt atgtgctcat ggatggctct 8880

attattcaat ttcctaacac ctaccttgaa ggttctgtaa gagtggtaac aacttttgat 8940

tctgagtact gtaggcacgg cacttgtgaa agatcagaag ctggtgtttg tgtatctact 9000

agtggtagat gggtacttaa caacgattat tacagatctt taccaggagt tttctgtggt 9060

gtagatgctg taaatttgct tactaacatg tttacaccac taattcaacc tattggtgct 9120

ttggacatat cagcatctat agtagctggt ggtattgtag ctatcgtagt aacatgcctt 9180

gcctactatt ttatgaggtt tagacgtgct tttggtgaat acagtcatgt agttgccttt 9240

aatactctcc tattccttat gtcattcact gtactctgtt taacaccagt ttactcattc 9300

ttacctggtg tttattctgt tatttacctg tacttgacat tttatctgac taatgatgtt 9360

tcttttctcg cacatattca gtggatggtt atgttcacac ctttagtacc tttctggata 9420

acaattgctt acatcatttg tatttccaca aagcatttct attggttctt tagtaattac 9480

ctaaagagac gtgtagtctt taatggtgtt tcctttagta cttttgaaga agctgcgctg 9540

tgcacctttt tgttaaataa ggagatgtat ctaaagttgc gtagtgatgt gctattacct 9600

cttacgcaat ataatagata cttagctctt tataacaagt acaagtattt cagtggagca 9660

atggatacaa ctagctacag agaagctgct tgttgtcatc tcgcaaaggc tctcaatgac 9720

ttcagtaact caggttctga tgttctttac caaccaccac aaacctctat cacctcagct 9780

gttttgcaga gtggttttag aaaaatggca ttcccatctg gtaaagttga gggttgtatg 9840

gtacaagtaa cttgtggtac aactacactt aacggtcttt ggcttgatga cgtagtttac 9900

tgtccaagac atgtgatctg cacctctgaa gatatgctta accctaatta tgaagatcta 9960

ctcatccgta agtctaatca taacttcttg gtacaggctg gtaatgttca actcagggtt 10020

attggacatt ctatgcaaaa ttgtgtactt aagcttaagg ttgatacagc caatcctaag 10080

acacctaagt ataagtttgt tcgcattcaa ccaggacaga ctttttcagt gttagcttgt 10140

tacaatggtt caccatctgg tgtttaccaa tgtgctatga ggcccaattt cactattaag 10200

ggttcattcc ttaatggttc atgtggtagt gttggtttta acatagatta tgactgtgtc 10260

tctttttgtt acatgcacca tatggaatta ccaactggag ttcatgctgg cacagactta 10320

gaaggtaact tttatggacc ttttgttgac aggcaaacag cacaagcagc tggtacagat 10380

acaactatta cagttaatgt tcttgcttgg ttgtacgctg ctgttataaa tggagacagg 10440

tggtttctca atcgatttac cacaactctt aatgacttta accttgtggc tatgaagtac 10500

aattatgaac ctctaacaca agaccatgtt gacatactag gacctctttc tgctcaaact 10560

ggaattgccg ttttagatat gtgtgcttca ttaaaagaac ttctgcaaaa tggtatgaat 10620

ggacgtacca tattgggtag tgctttatta gaagatgagt ttacaccttt tgatgttgtt 10680

agacaatgct caggtgttac tttccaaagt gcagtgaaaa gaacaatcaa gggtacacac 10740

cactggttgt tactcacaat tttgacttca cttttagttt tagtccagag tactcaatgg 10800

tctttgttct ttttcttcta cgaaaatgcc tttttacctt ttgctatggg tattattgct 10860

atgtctgctt ttgcaatgat gtttgtcaaa cataagcatg catttctctg tttgtttttg 10920

ttaccttctc ttgccactgt agcttacttt aatatggtct acatgcctgc tagttgggtg 10980

atgcgtatta tgacatggtt ggatatggtt gatactagtt tgtctggttt taagctaaaa 11040

gactgtgtta tgtatgcatc agctgtagtg ttactaatcc ttatgacagc aagaactgtg 11100

tatgatgatg gtgctaggag agtgtggaca cttatgaatg tcttgacact cgtttataaa 11160

gtttactatg gcaacgcttt agatcaagcc atttccatgt gggctcttat aatctctgtt 11220

acttctaact actcaggtgt agttacaact gtcatgtttt tggccagagg tattgttttt 11280

atgtgtgttg agtattgccc tattttcttc ataactggta atacacttca gtgtataatg 11340

ctagtctatt gtttcttagg ctatttttgt acttgttact tcggcctctt ttgtttactc 11400

aaccgctact ttagactgac tcttggtgtt tatgattact tagtgtctac acaggagttt 11460

agatatatga attcacaggg actactccca cccaagaata gcatagatgc cttcaaactc 11520

aacattaaat tgttgggtgt tggtggcaaa ccttgtatca aagtagccac tgtacagtct 11580

aaaatgtcag atgtaaagtg cacatcagta gtcttactct cagttttgca acaactcaga 11640

gtagaatcat catctaaatt gtgggctcaa tgtgtccagt tacacaatga cattctctta 11700

gctaaagata ctactgaagc ctttgaaaaa atggtttcac tactttctgt tttgctttcc 11760

atgcagggtg ctgtagacat aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc 11820

ttacaagcta tagcctcaga gtttagttcc cttccatcat atgcagcttt tgctactgct 11880

caagaagctt atgagcaggc tgttgctaat ggtgattctg aagttgttct taaaaagttg 11940

aagaagtctt tgaatgtggc taaatctgaa tttgaccgtg atgcagccat gcaacgtaag 12000

ttggaaaaga tggctgatca agctatgacc caaatgtata aacaggctag atctgaggac 12060

aagagggcaa aagttactag tgctatgcag acaatgcttt tcactatgct tagaaagttg 12120

gataatgatg cactcaacaa cattatcaac aatgcaagag atggttgtgt tcccttgaac 12180

ataatacctc ttacaacagc agccaaacta atggttgtca taccagacta caacacatat 12240

aagaatacgt gtgatggtac aacatttact tatgcatcag cattgtggga aatccaacag 12300

gttgtagatg cagatagtaa aattgttcag cttagtgaaa ttagtatgga caattcacct 12360

aatttagcat ggcctcttat tgtaacagct ttaagggcca attctgctgt caaattacag 12420

aataatgagc ttagtcctgt tgcactaaga caaatgtctt gtgctgccgg tactacacaa 12480

actgcttgca ctgatgacaa tgcgttagct tactacaaca caacaaaggg aggtaggttt 12540

gtacttgcac tgttatccga tttacaggat ttgaaatggg ctagattccc taagagtgat 12600

ggaactggta ctatctatac agaactggaa ccaccttgta ggtttgttac agacacacct 12660

aaaggtccta aagtgaagta tctttacttc atcaaaggat taaacaacct aaatagaggt 12720

atggtacttg gtagtttagc tgccacagta cgtttacaag ctggtaatgc aacagaagtt 12780

cctgctaatt caactgtact ttctttctgt gcttttgctg tagatgctgc taaagcttac 12840

aaagattatc tagctagtgg gggacaacca atcactaatt gtgttaagat gttgtgtaca 12900

cacactggta ctggtcaggc aataacagtt acaccggaag ccaatatgga tcaagaatcc 12960

tttggtggtg catcgtgttg tctgtactgc cgttgtcata tagatcatcc aaatcctaaa 13020

ggattttgtg acttaaaagg taagtatgta caaataccta caacttgtgc taatgaccct 13080

gtgggtttta cacttaaaaa cacagtctgt accgtctgcg gtatgtggaa aggttatggt 13140

tgtagttgtg atcaactccg cgaacccatg cttcagtcag ctgatgcaca atcgttttta 13200

aacgggtttg cggtgtaagt gcagcccgtc ttacaccgtg cggcacaggc actagtactg 13260

atgtcgtata tagagctttt gacatctaca atgataaagt agctggtttt gctaagttcc 13320

taaaaactaa ttgttgtcgc ttccaagaaa aggacgaaga tgacaatctc attgattctt 13380

actttgtagt taagagacac actttctcta actaccaaca tgaagaaaca atttacaacc 13440

tgcttaagga ttgtccagct gttgctaaac atgacttctt taagtttaga atagacggtg 13500

acatggtacc acatatatca cgtcaacgtc ttactaaata cacaatggca gacctcgtct 13560

atgctttaag gcattttgat gaaggtaatt gtgacacatt aaaagaaata cttgtcacat 13620

acaattgttg tgatgatgac tacttcaata aaaaggactg gtatgatttt gtagaaaacc 13680

cagatatatt acgcgtatac gccaacttag gtgaacgtgt acgccaagct ttgttaaaaa 13740

cagtacagtt ctgtgatgcc atgcgaaatg ctggtattgt tggtgtactg acattagata 13800

atcaagatct caatggtaac tggtatgact ttggtgattt catacaaacc acgccaggta 13860

gtggagttcc tgttgtagac tcttattatt cattgctcat gcctatatta accttgacca 13920

gggctttaac tgcagagtca catgttgaca ctgacttaac aaagccttac attaagtggg 13980

atttgttaaa atacgacttc acggaagaga ggttaaaact ctttgaccgt tattttaaat 14040

actgggatca gacataccac ccaaattgtg ttaactgttt ggatgacaga tgcattctgc 14100

attgtgcaaa ctttaatgtt ctgttctcta cagtgttccc acctacaagt tttggaccac 14160

tagtgagaaa aatatttgtt gatggtgttc catttgtagt ttcaactgga taccacttca 14220

gagagctagg tgttgtacat aatcaggatg taaacttaca tagctctaga cttagtttta 14280

aggaattact tgtgtatgct gctgatcctg ctatgcatgc tgcttctggt aatctattac 14340

tagataaacg cactacgtgc ttttcagtag ctgcacttac taacaatgtt gcttttcaaa 14400

ctgtcaaacc cggtaatttt aacaaggact tctatgactt tgctgtgtct aagggtttct 14460

ttaaggaagg aagttctgtt gaattaaaac acttcttctt tgctcaggat ggtaatgctg 14520

ctatcagcga ttatgactac tatcgttata atctaccaac aatgtgtgat atcagacaac 14580

tactatttgt agttgaagtt gttgataagt actttgattg ttacgatggt ggctgtatta 14640

atgctaacca agtcatcgtc aacaacctag acaaatcagc tggttttcca tttaataaat 14700

ggggtaaggc tagactttat tatgattcca tgagttatga ggatcaagat gcacttttcg 14760

catatacaaa acgtaatgtc atccctacta taactcaaat gaaccttaag tatgccatta 14820

gtgcaaagaa tagagctcgc accgtagctg gtgtctctat ctgtagtact atgaccaata 14880

gacagtttca tcaaaaatta ctcaagtcaa tagccgccac tagaggagct actgtagtaa 14940

ttggaacaag caaattctat ggtggttggc acaacatgct caaaactgtt tatagtgatg 15000

tagaaaaccc tcaccttatg ggttgggatt atcctaaatg tgatagagcc atgcctaaca 15060

tgcttagaat tatggcctca cttgttcttg ctcgcaaaca tacaacgtgt tgtagcttgt 15120

cacaccgttt ctatagatta gctaatgagt gtgctcaagt attgagtgaa atggtcatgt 15180

gtggcggttc actatatgtt aaaccaggtg gaacctcatc aggagatgcc acaactgctt 15240

atgctaatag tgtgtttaac atttgtcaag ctgtcacggc caatgttaat gcacttttat 15300

ctactgatgg taacaaaatt gccgataagt atgtccgcaa tttacaacac agactttatg 15360

agtgtctcta tagaaataga gatgttgaca cagactttgt gaatgagttt tacgcatatt 15420

tgcgtaaaca tttctcaatg atgatactct ctgacgatgc tgttgtgtgt ttcaatagca 15480

cttatgcatc tcaaggtcta gtggctagca taaagaactt taagtcagtt ctttactatc 15540

aaaacaacgt ttttatgtct gaagcaaaat gttggactga gactgacctt actaaaggac 15600

ctcatgaatt ttgctctcaa catacaatgc tagttaaaca gggtgatgat tatgtgtacc 15660

ttccttaccc agatccatca agaatcctag gtgccggttg ttttgtagat gatatcgtaa 15720

aaacagatgg tacacttatg attgaacggt tcgtgtcttt agctatagat gcttacccac 15780

ttactaaaca tcctaatcag gagtatgctg atgtctttca tttgtactta caatacatac 15840

gtaagctaca tgatgagtta acaggacaca tgttagacat gtattctgtt atgcttacta 15900

atgataacac ttcaaggtat tgggaacctg agttttatga ggctatgtac acaccgcata 15960

cagtcttaca agctgttggt gcttgtgttc tttgcaattc acagacttca ttaagatgtg 16020

gtgcttgcat acgtagacca ttcttatgtt gtaaatgctg ttacgaccat gtcatctcaa 16080

catcacataa attagtcttg tctgttaatc cgtatgtttg caatgctcca ggttgtgatg 16140

tcacagatgt gactcaactt tacttaggag gtatgagcta ttactgtaag tcacataaac 16200

cacccattag ttttccattg tgtgctaatg gacaagtttt tggtctctac aagaatacat 16260

gtgttggtag cgataatgtt actgacttta atgcaattgc aacatgtgac tggacaaatg 16320

ctggtgatta cattttagct aacacctgta ctgaaagact caagcttttt gcagcagaaa 16380

cgctcaaagc tactgaggag acatttaaac tgtcttatgg tattgctact gtacgtgaag 16440

tgctgtctga cagagaatta catctttcat gggaagttgg taaacctaga ccaccactta 16500

accgaaatta tgtctttact ggttatcgtg taactaaaaa cagtaaagtg caaatcggag 16560

agtacacctt tgaaaaaggt gactatggtg atgctgttgt ttaccgaggt acaacaactt 16620

acaaactcaa cgttggtgat tattttgtgc tgacatcaca tacagtaatg ccattaagtg 16680

cacctacact agtgccacaa gagcactatg ttagaattac tggcttatac ccaacactca 16740

atatctcaga tgagttttct agcaatgttg caaattatca aaaggttggt atgcaaaagt 16800

attctacact ccagggacca cctggtactg gtaaaagtca ttttgctatt ggtctagctc 16860

tctactaccc ttctgctcgc atagtatata cagcttgctc tcatgcagct gttgatgcac 16920

tatgtgagaa ggcattaaaa tatttgccca tagacaaatg tagtagaatt atacctgcac 16980

gtgctcgtgt agagtgtttt gataaattca aggtgaattc aacattagaa cagtatgtct 17040

tttgtactgt aaatgcattg cctgagacga cagcagatat agttgtcttt gatgaaattt 17100

caatggccac aaattatgat ttgagtgttg tcaatgccag attacgtgct aagcactatg 17160

tgtacattgg tgatcctgct caattacctg caccacgcac attactaact aagggtacac 17220

tagaaccaga atatttcaat tcagtgtgta gacttatgaa aactataggt ccagacatgt 17280

tcctcggaac ttgtcgtaga tgtcctgctg aaattgttga cactgtgagt gctttggttt 17340

atgataataa gcttaaggca cataaagaca aatcagctca atgctttaaa atgttctaca 17400

agggtgttat cacgcatgat gtttcatctg caattaacag gccacaaata ggcgtggtaa 17460

gagaattcct tacacgtaac cctgcttgga gaaaagctgt ctttatttca ccttacaatt 17520

cccagaatgc tgtagcctca aagattttgg gactaccaac tcaaactgtt gattcatcac 17580

agggctcaga atatgactat gtcatattca ctcaaaccac tgaaacagct cactcttgta 17640

atgtaaacag attcaacgtt gctattacca gagcaaaagt aggcatactt tgcataatgt 17700

ctgatagaga cctttatgac aagttgcaat ttacaagtct tgaaattcca cgtaggaatg 17760

tggcaacttt acaagctgaa aatgtaacag gactctttaa agattgtagt aaggtaatca 17820

ctgggttaca tcctacacag gcacctacac acttaagtgt tgatactaaa ttcaaaactg 17880

aaggtttatg tgttgacata cctggcatac ctaaggacat gacctataga agattaatct 17940

ctatgatggg tttcaaaatg aattaccagg ttaatggtta ccctaacatg tttatcaccc 18000

gcgaagaagc tataagacat gtacgtgcat ggattggctt cgatgtcgaa ggttgtcatg 18060

ctactagaga agctgttggt accaatttac ctttacagct aggtttttct acaggtgtta 18120

acctagttgc tgtacctaca ggttatgttg atacacctaa taatacagat ttttccagag 18180

ttagtgctaa accaccgcct ggagatcaat ttaaacacct cataccactt atgtacaaag 18240

gacttccttg gaatgtagtg cgtataaaga ttgtccaaat gttaagtgac acacttaaaa 18300

atctctctga cagagtcgta tttgtcttat gggcacatgg ctttgagttg acatctatga 18360

agtattttgt gaagatcgga cctgagcgca catgttgtct atgtgataga cgtgctacat 18420

gcttttccac tgcttcagac acttatgcct gttggcatca ttctattgga tttgattacg 18480

tctataatcc gtttatgatt gatgttcaac aatggggttt tacaggtaac ctacaaagca 18540

accatgatct gtattgtcaa gtccatggta atgcacatgt agctagttgt gatgcaatca 18600

tgactaggtg tctagctgtc cacgagtgct ttgttaagcg tgttgactgg actattgaat 18660

atcctataat cggtgatgaa ctgaagatta atgcggcttg tagaaaggtt caacacatgg 18720

ttgttaaagc tgcattatta gcagacaaat tcccagttct tcacgacatt ggtaacccta 18780

aagctattaa gtgtgtacct caagctgatg tagaatggaa gttctatgat gcacagcctt 18840

gtagtgacaa agcttacaaa atagaagaac tgttctattc ttatgccaca cattctgaca 18900

aattcacaga tggtgtatgc ctattttgga attgcaatgt cgatagatat cctgctaatt 18960

ccattgtttg tagatttgac actagagtgc tatctaacct taacttgcct ggttgtgatg 19020

gtggcagttt gtatgtaaat aagcatgcat tccacacacc agcttttgat aaaagtgctt 19080

ttgttaatct aaagcaactt ccatttttct attactctga cagtccatgt gagtctcatg 19140

gaaaacaagt agtgtcagat atagattatg taccactaaa gtctgctacg tgtataacac 19200

gttgcaattt aggtggtgct gtctgtagac atcatgctaa tgagtacaga ttgtatctcg 19260

atgcttataa catgatgatc tcagctggct ttagcttgtg ggtttacaaa caatttgata 19320

cctataacct ctggaacact tttacaagac ttcagagttt agaaaatgtg gcttttaatg 19380

ttgtaaataa gggacacttt gatggacaac agggtgaagt accagtttct atcattaaca 19440

acactgttta cacaaaagtt gatggtgttg atgtagaatt gtttgagaac aaaaccacat 19500

tacctgttaa tgtagcattt gagctttggg ctaagcgcaa cattaaacca gtaccagagg 19560

tgaaaatact caataatttg ggtgtggaca ttgctgctaa tactgtgatc tgggactaca 19620

aaagagatgc tccagcacat atatctacta ttggtgtttg ttctatgact gacatagcca 19680

agaaaccaac tgaaacgatt tgtgcaccac tcactgtctt ttttgatggt agagttgatg 19740

gtcaagtaga cttatttaga aatgcccgta atggtgttct tattacagaa ggtagtgtta 19800

aaggtttaca accatctgta ggtcccaaac aagctagtct taatggagtc acattaattg 19860

gagaagccgt aaaaacacag ttcaattatt acaagaaagt ggatggtgtt gtccaacaat 19920

tacctgaaac ttactttact cagagtagaa acttacagga atttaagccc aggagtcaaa 19980

tggaaattga tttcttagaa cttgctatgg atgaattcat tgaacggtat aaattagaag 20040

gctatgcctt cgaacatatc gtttatggag attttagtca tagtcagtta ggtggtttac 20100

atctactgat tggactagct aaacgtttta aggaatcacc ttttgaactt gaagatttta 20160

ttcctatgga cagtacagtt aaaaactact tcataacaga tgcgcaaaca ggttcatcta 20220

agtgtgtgtg ttctgttatt gatcttttac ttgatgactt cgttgaaata ataaagtccc 20280

aagatttatc tgtagtttct aaggttgtca aagtgactat tgactataca gaaatctcat 20340

ttatgctttg gtgtaaagat ggccatgtag aaacatttta cccaaaatta caatctagtc 20400

aagcgtggca accgggtgtt gctatgccta atctttacaa aatgcaaaga atgctattag 20460

aaaagtgtga ccttcaaaat tatggtgata gtgcaacatt acctaaaggc ataatgatga 20520

atgtcgcaaa atatactcaa ctgtgtcaat atttaaacac actgacatta gctgtaccct 20580

ataatatgag agttatccat tttggtgctg gttctgataa aggagttgca ccaggtacag 20640

ctgttttaag acaatggttg cctacaggta cgctgcttgt cgattcagat cttaatgact 20700

ttgtctctga tgcagattca actttgattg gtgattgtgc aactgtacat acagctaata 20760

aatgggatct cattattagt gatatgtacg accctaagac taagaatgtc acaaaagaaa 20820

acgactctaa agagggtttt ttcacttaca tttgtgggtt tatacaacaa aagctagctc 20880

ttggaggttc cgtggctata aagataacag aacattcttg gaatgctgat ctttataagc 20940

tcatgggaca cttcgcatgg tggacagcct ttgttactaa tgtgaatgcg tcatcatctg 21000

aagcattttt aatcggatgt aactaccttg gcaaaccacg cgaacaaata gatggttatg 21060

tcatgcatgc aaattacata ttttggagga atacaaatcc aattcagctt tcttcttatt 21120

ctttattcga catgagtaaa ttccccctta aattaagggg tactgctgtt atgtctttaa 21180

aagaaggtca aatcaatgat atgattctct ctcttcttag taaaggtaga cttataatta 21240

gagaaaacaa cagagttgtt atttctagtg atgttcttgt taacaactaa 21290

<210> 52

<211> 828

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 ORF3a

<400> 52

atggatttgt ttatgagaat cttcacaatt ggaactgtaa ctttgaagca aggtgaaatc 60

aaggatgcta ctccttcaga ttttgttaga gctactgcaa cgataccgat acaagcatca 120

cttcctttcg gatggcttat tgttggcgtt gcacttcttg ctgtttttca gagcgcttcc 180

aaaatcataa ccctcaaaaa gagatggcaa ctagcactct ccaagggtgt tcactttgtt 240

tgcaacttgc tgttgttgtt tgtaacagtt tactcacatc ttttgcttgt tgctgctggc 300

cttgaagccc cttttctcta tctttatgct ttagtctact tcttgcagag tataaacttt 360

gtacgcataa taatgaggct ttggctttgc tggaaatgcc gttccaaaaa cccattactt 420

tatgatgcca actattttct ttgctggcat actaattgtt acgactattg tataccttac 480

aatagtgtaa cttcttcaat tgtcattact tcaggtgatg gcacaacaag tcctatttct 540

gaacatgact accagattgg tggttatact gaaaaatggg aatctggagt aaaagactgt 600

gttgtattac acagttactt cacttcagac tattaccagc tgtactcaac tcaattgagt 660

acagacactg gtgttgaaca tgttaccttc ttcatctaca ataaaatcgt tgatgagcct 720

gaagaacatg tccaaattca cacaatcgac gtttcatccg gagttgttaa tccagtaatg 780

gaaccaattt atgatgaacc gacgacgact actagcgtgc ctttgtaa 828

<210> 53

<211> 186

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 ORF6

<400> 53

atgtttcatc tcgttgactt tcaggttact atagcagaga tattactaat catcatgagg 60

acttttaaag tttccatttg gaatcttgat tacatcataa acctcataat taagaactta 120

agcaagtcac taactgagaa taaatattct caactagacg aggagcagcc aatggagatt 180

gattaa 186

<210> 54

<211> 366

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 ORF7a

<400> 54

atgaaaatta ttcttttctt ggcactgata acactcgcta cttgtgagct ttatcactac 60

caagagtgtg ttagaggtac aacagtactt ttaaaagaac cttgctcgtc gggaacatac 120

gagggcaatt caccatttca tcctctagct gataacaaat ttgcactgac ttgctttagc 180

actcaatttg cttttgcttg tcctgacggc gtaaaacacg tctatcagtt acgtgccaga 240

tcagtttcac ctaaactgtt catcagacaa gaggaagttc aagaacttta ctctccaatt 300

tttcttattg ttgcggcaat agtgtttata acactttgct tcacactcaa aagaaagaca 360

gaatga 366

<210> 55

<211> 366

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 ORF8

<400> 55

atgaaatttc ttgttttctt aggaatcatc acaactgtag ctgcatttca ccaagaatgt 60

agtttacagt catgtactca acatcaacca tatgtagttg atgacccgtg tcctattcac 120

ttctattcta aatggtatat cagagtagga gctagaaaat cagcaccttt aattgaattg 180

tgcgtggatg aggctggttc taaatcaccc attcagtaca tcgatatcgg taattataca 240

gtttcctgtt taccttttac aattaactgc caggaaccta aattgggtag tcttgtagtg 300

cgttgttcgt tctacgagga ctttttagag tatcatgacg ttcgtgttgt tttagatttc 360

atctaa 366

<210> 56

<211> 265

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 5'UTR

<400> 56

attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60

gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120

cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180

ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240

cgtccgggtg tgaccgaaag gtaag 265

<210> 57

<211> 206

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 3'UTR

<400> 57

caatctttaa tcagtgtgta acattaggga ggacttgaaa gagccaccac attttcaccg 60

aggccacgcg gagtacgatc gagtgtacag tgaacaatgc tagggagagc tgcctatatg 120

gaagagccct aatgtgtaaa attaatttta gtagtgctat ccccatgtga ttttaatagc 180

ttcttaggag aatgacaaaa aaaaac 206

<210> 58

<211> 13203

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 orf1a

<400> 58

atggagagcc ttgtccctgg tttcaacgag aaaacacacg tccaactcag tttgcctgtt 60

ttacaggttc gcgacgtgtt agtacgtggt tttggagatt cagtggaaga agtcttatca 120

gaggcacgtc aacatcttaa agatggcact tgtggcttag tagaagttga aaaaggcgtt 180

ttgcctcaac ttgaacagcc ctatgtgttc atcaaacgtt ctgatgctag aactgcacct 240

catggtcatg ttatggttga gctggtagca gaattagaag gtattcagta cggtcgtagt 300

ggtgagacat taggtgtttt agttcctcat gtgggcgaaa taccagtggc ttaccgcaaa 360

gttcttctta gaaagaacgg taataaagga gctggtggcc atagttacgg cgctgattta 420

aagtcatttg acttaggcga cgagcttggc actgatcctt atgaagattt ccaagaaaac 480

tggaacacta aacatagcag tggtgttacc cgtgaactca tgcgtgagtt aaatggaggt 540

gcatacactc gctatgtcga taacaacttc tgtggacctg atggttaccc tcttgagtgc 600

attaaagacc ttctagcacg tgctggtaaa gcttcatgca ctttgtccga acaactggac 660

tttattgaca ctaagagggg tgtatactgc tgccgtgaac atgagcatga aattgcttgg 720

tacacggaac gttctgaaaa gagctatgaa ttgcagacac cttttgaaat taaactggca 780

aagaaatttg acaccttcaa tggggaatgt ccaaattttg tatttcccct caattccata 840

atcaagacta ttcaaccaag ggttgaaaag aaaaagcttg atggctttat gggtagaatt 900

cgatctgtct atccagttgc gtcaccaaat gaatgcaacc aaatgtgcct ttcaactctc 960

atgaagtgtg atcattgtgg tgaaacttca tggcagacgg gcgattttgt taaagccact 1020

tgcgaatttt gtggcactga gaatttgact aaagaaggtg ccactacttg tggttactta 1080

ccccaaaatg ctgttgttaa aatttactgt ccagcatgtc acaattcaga agtaggacct 1140

gagcatagtc ttgccgaata ccataatgaa tctggcttga aaaccattct tcgtaagggt 1200

ggtcgcacta ttgcttttgg aggatgtgtg ttctcttatg ttggttgcca taacaagtgt 1260

gcttattggg ttccacgtgc ttcagctaac ataggttgta accatacagg tgttgttgga 1320

gaaggttccg aaggtcttaa tgacaacctt cttgaaatac tccaaaaaga gaaagtcaac 1380

atcaatattg ttggtgactt taaacttaat gaagagatcg ccattatttt ggcatctttt 1440

tctgcttcca caagtgcttt tgtggaaact gtgaaaggtt tggattataa agcattcaaa 1500

cagattgttg aatcctgtgg taattttaag gttacaaagg gaaaagctaa aaaaggtgcc 1560

tggaatattg gtgaacagaa atcaatactg agtcctcttt atgcatttgc atcagaggct 1620

gctcgtgttg tacgatcaat tttctcccgc actcttgaaa ctgctcaaaa ttctgtgcgt 1680

gttttacaga aggccgctat aacaatacta gatggaattt cacagtattc actgagactc 1740

attgatgcta tgatgttcac atctgatttg gctactaaca atctagttgt aatggcctac 1800

attacaggtg gtgttgttca gttgacttcg cagtggctaa ctaacatctt tggcactgtt 1860

tatgaaaaac tcaaacccgt ccttgattgg cttgaagaga agtttaagga aggtgtagag 1920

tttcttagag acggttggga gattgttaaa ttcatctcaa cctgtgcttg tgaaattgtc 1980

ggtggacaaa ttgtcacctg tgctaaggaa attaaggaga gtgttcagac attctttaag 2040

cttgtaaaca agtttttggc tttgtgtgct gactctatca ttattggtgg agctaaactt 2100

aaagccttga atttaggtga aacatttgtc acgcactcaa agggattgta cagaaagtgt 2160

gttaaatcca gagaagaaac tggcctactc atgcctctaa aagccccaaa agaaattatc 2220

ttcttagagg gagaaacact tcccacagaa gtgttaacag aggaagttgt cttgaaaact 2280

ggtgatttac aaccattaga acaacctact agtgaagctg ttgaagctcc attggttggt 2340

acaccagttt gtattaacgg gcttatgttg ctcgaaatca aagacacaga aaagtactgt 2400

gcccttgcac ctaatatgat ggtaacaaac aataccttca cactcaaagg cggtgcacca 2460

acaaaggtta cttttggtga tgacactgtg atagaagtgc aaggttacaa gagtgtgaat 2520

atcacttttg aacttgatga aaggattgat aaagtactta atgagaagtg ctctgcctat 2580

acagttgaac tcggtacaga agtaaatgag ttcgcctgtg ttgtggcaga tgctgtcata 2640

aaaactttgc aaccagtatc tgaattactt acaccactgg gcattgattt agatgagtgg 2700

agtatggcta catactactt atttgatgag tctggtgagt ttaaattggc ttcacatatg 2760

tattgttctt tctaccctcc agatgaggat gaagaagaag gtgattgtga agaagaagag 2820

tttgagccat caactcaata tgagtatggt actgaagatg attaccaagg taaacctttg 2880

gaatttggtg ccacttctgc tgctttacaa cctgaagaag aacaagaaga agattggtta 2940

gatgatgata gtcaacaaac tgttggtcaa caagacggca gtgaggacaa tcagacaact 3000

actattcaaa caattgttga ggttcaacct caattagaga tggaacttac accagttgtt 3060

cagactattg aagtgaatag ttttagtggt tatcttaaac ttactgacaa tgtatacatc 3120

aagaatgcag acattgtgga agaagctaaa aaggtaaaac caacagtggt tgttaatgca 3180

gccaatgttt accttaaaca tggaggaggt gttgcaggag ccttaaataa ggctactaac 3240

aatgccatgc aagttgaatc tgatgattac atagctacta atggaccact taaagtgggt 3300

ggtagttgtg ttttaagcgg acacaatctt gctaaacact gtttacatgt tgtcggccca 3360

aatgttaaca aaggtgaaga tattcaactt cttaagagtg cttatgaaaa ttttaaccag 3420

cacgaagttc tacttgcacc attattatca gctggtattt ttggtgctga ccctatacat 3480

tctttaagag tttgtgtaga tactgttcgc acaaatgtct acttagctgt ctttgataaa 3540

aatctctatg acaaacttgt ttcaagcttt ttggaaatga agagtgaaaa gcaagttgaa 3600

caaaagatcg ctgagattcc taaagaggaa gttaagccat ttataactga aagtaaacct 3660

tcagttgaac agagaaaaca agatgataag aagatcaaag cttgtgttga agaagttaca 3720

acaactctgg aagaaactaa gttcctcaca gaaaacttgc tcctttatat cgacattaat 3780

ggcaatcttc atccagattc tgccactctt gttagtgaca ttgacatcac tttcttaaag 3840

aaagatgctc catatatagt gggtgatgtt gttcaagagg gtgttttaac tgctgtggtt 3900

atacctacta aaaaggctgg tggcactact gaaatgctag cgaaagcttt gagaaaagtg 3960

ccaacagaca attatataac cacttacccg ggtcagggtt taaatggtta cactgtagag 4020

gaggcaaaga cagtgcttaa aaagtgtaaa agtgcctttt acattctacc atctattatc 4080

tctaatgaga agcaagaaat tcttggaact gtttcttgga atttgcgaga aatgcttgca 4140

catgcagaag aaacacgcaa attaatgcct gtctgtgtgg aaactaaagc catagtttca 4200

actatacagc gtaaatataa gggtatcaag atacaagagg gtgtggttga ttatggtgct 4260

agattttact tttacaccag taaaacaact gtagcgtcac ttatcaacac acttaacgat 4320

ctaaatgaaa ctcttgttac aatgccactt ggctatgtaa cacatggctt aaatttggaa 4380

gaagctgctc ggtatatgag atctctcaaa gtgccagcta cagtttctgt ttcttcacct 4440

gatgctgtta cagcgtataa tggttatctt acttcttctt ctaaaacacc tgaagaacat 4500

tttattgaaa ccatctcact tgctggttcc tataaagatt ggtcctattc tggacaatct 4560

acacaactag gtatagaatt tcttaagaga ggtgataaaa gtgtatatta cacgtccaat 4620

cctaccacat tccacctaga tggtgaagtt atcacctttg acaatcttaa gacacttctt 4680

tctttgagag aagtgaggac tattaaggtg tttacaacag tagacaacat taacctccac 4740

acgcaagttg tggacatgtc aatgacatat ggacaacagt ttggtccaac ttatttggat 4800

ggagctgatg ttactaagat aaaacctcat aactcacatg aaggtaaaac attttacgtt 4860

ttgcctaatg atgacactct acgtgttgag gcttttgagt actaccacac aactgatcct 4920

agttttctgg gtaggtacat gtcagcatta aatcacacta aaaagtggaa atacccacaa 4980

gttaatggtt taacttcgat taaatgggca gataacaact gttatcttgc cactgcattg 5040

ttaacactcc aacaaataga gttgaagttt aatccacctg ctctacaaga tgcttattac 5100

agagcaaggg ctggtgaagc tgctaacttt tgtgcactta tcttagccta ctgtaataag 5160

acagtaggtg agttaggtga tgttagagaa acaatgagtt acttgtttca acatgccaat 5220

ttagattctt gcaaaagagt cttgaacgtg gtgtgtaaaa cttgtggaca acagcagaca 5280

acccttaagg gtgtagaagc tgttatgtac atgggcacac tttcttatga acaattcaag 5340

aaaggtgttc agataccttg tacgtgtggt aaacaagcta caaaatatct agtacaacag 5400

gagtcacctt ttgttatgat gtcagcacca cctgctcagt atgaacttaa gcatggtaca 5460

tttacttgtg ctagtgagta cactggtaat taccagtgtg gtcactataa gcatataact 5520

tctaaggaaa ctttgtattg catagacggt gctttactta caaagtcctc agaatacaaa 5580

ggtcctatta cggatgtttt ctacaaagaa aacagttaca caacaaccat aaaaccagtt 5640

acttataagt tggatggtgt tgtttgtaca gaaattgacc ctaagttgga caattattat 5700

aagaaggaca actcttattt cacagagcaa ccaattgatc ttgtaccaaa ccaaccatat 5760

ccaaacgcaa gcttcgataa ttttaagttc gtatgcgata atatcaaatt tgctgatgat 5820

ctcaaccagt taactggtta taagaaacct gcttcaagag agcttaaagt tacatttttc 5880

cctgacttaa atggtgatgt ggtggctatt gattataaac actacacacc ctcttttaag 5940

aaaggagcta aattgttaca taagcctatt gtttggcatg ttaacaatgc aactaataaa 6000

gccacgtata aaccaaatac ctggtgtata cgttgtcttt ggagcacaaa accagttgaa 6060

acatcaaatt cgtttgatgt actgaagtca gaggacgcgc agggaatgga taatcttgca 6120

tgtgaagatc taaaaccagt ctctgaagaa gtagtggaaa atcctaccat acagaaagac 6180

gttcttgagt gtaatgtgaa aactaccgaa gttgtaggag acattatact taaaccagca 6240

aataatagtt tgaagatcac agaagaggtt ggccacacag atctaatggc tgcttatgta 6300

gacaattcta gtcttactat taagaaacct aatgaactct ctagagtatt aggtttgaaa 6360

acccttgcta ctcatggttt agctgctgtt aatagtgtcc cttgggatac tatagctaat 6420

tatgctaagc cttttcttaa caaagttgtt agtacaacta ctaacatagt tacacggtgt 6480

cttaatcgtg tttgtactaa ttatatgcct tacttcttta ctttattgct acaattgtgt 6540

acttttacta gaagtacaaa ttctagaatc aaggcatcta tgccgactac tatagcaaag 6600

aatactgtta agagtgtcgg taaattttgt ctagaggctt catttaatta tctcaagtca 6660

cctaactttt ctaagctgat aaacattatc atctggtttt tgctattaag tgtttgccta 6720

ggttctttaa tctactcaac cgctgcttta ggtgttttaa tgtctaattt aggcatgcct 6780

tcttactgta ctggttacag agaaggctat ttgaactcta ctaatgtcac tattgcaacc 6840

tactgtactg gatctatacc ttgtagtgtt tgtcttagtg gtttagattc tttagacacc 6900

tatccttctc ttgaaactat acagattacc atttcatctt tcaaatggga tttaactgct 6960

tttggcttag ttgcagagtg gtttttggca tatattcttt tcactaggtt tttctatgta 7020

cttggattgg ctgcaatcat gcaattgttt ttcagctatt ttgcagtcca ttttattagt 7080

aactcttggc ttatgtggct tataattaat cttgtgcaga tggccccgat ttcagctatg 7140

gttagaatgt acatcttctt tgcctcattt tattatgtgt ggaaaagtta tgtgcatgtt 7200

gtagacggtt gtaattcatc aacttgtatg atgtgttaca aacgtaatag agcaacaaga 7260

gtcgaatgta caactattgt taatggtgtt agaaggtcct tttatgtcta tgctaatgga 7320

ggtaaaggct tttgcaaact acacaattgg aattgtgtta attgtgatac attctgtgct 7380

ggtagtacat ttattagtga tgaagttgcg agagacttgt cactacagtt taaaagacca 7440

ataaatccta ctgaccaatc ttcttacatc gttgatagtg ttacagtgaa gaatggttcc 7500

atccatcttt actttgataa agctggtcaa aagacttatg aaagacattc tctctctcat 7560

tttgttaact tagacaacct gagagctaat aacactaaag gttcattgcc tattaatgtt 7620

atcgttttcg acggtaaatc aaaatgtgaa gaatcatctg caaaatcagc gtctgtttac 7680

tacagtcagc ttatgtgtca acctatactg ttactagatc aggcattagt gtctgatgtt 7740

ggtgatagtg cggaagttgc agttaaaatg tttgatgctt acgttaatac gttttcatca 7800

acttttaacg taccaatgga aaaactcaaa acactagttg caactgcaga agctgaactt 7860

gcaaagaatg tgtccttaga caatgtctta tctacgttta tttcagcagc tcggcaaggg 7920

tttgttgatt cagatgtaga aactaaagat gttgttgaat gtcttaaatt gtcacatcaa 7980

tctgacatag aagttactgg cgatagttgt aataactata tgctcaccta taacaaagtt 8040

gaaaacatga caccccgtga ccttggtgct tgtattgact gtagtgctag acatattaat 8100

gcgcaggtag caaaaagtca caacattgct ttgatatgga acgttaaaga tttcatgtca 8160

ttgtctgaac aactacgaaa acaaatacgt agtgctgcta aaaagaataa cttacccttc 8220

aagttgacat gtgcaactac tagacaagtt gttaatgttg taacaacaaa gatagcactt 8280

aagggtggta aaattgtgaa taactggttg aagcagctta ttaaagttac acttgtgttc 8340

ctttttgttg ctgctatttt ctatctgata acacctgttc atgtcatgtc taaacatact 8400

gacttttcaa gtgaaatcat aggatacaag gctattgatg gtggtgtcac tcgtgacata 8460

gcatctacag atacttgttt tgctaacaaa catgctgatt ttgacacatg gtttagccag 8520

cgtggtggta gttatactaa tgacaaagct tgcccattga ttgctgcagt cataacaaga 8580

gaagtgggtt ttgtcgttcc tggtttgcct ggaacgatat tacgcacaac taatggtgac 8640

tttttgcatt tcttacctag agtttttagt gcagttggta acatctgtta cacaccatca 8700

aaacttatag agtacactga ctttgcaaca tcagcttgtg ttttggctgc tgaatgtaca 8760

atttttaaag acgcttctgg taagccagta ccatattgtt atgataccaa tgtactagaa 8820

ggttctgttg cttatgaaag tttacgccct gacacacgtt atgtgctcat ggatggctct 8880

attattcaat ttcctaacac ctaccttgaa ggttctgtaa gagtggtaac aacttttgat 8940

tctgagtact gtaggcacgg cacttgtgaa agatcagaag ctggtgtttg tgtatctact 9000

agtggtagat gggtacttaa caacgattat tacagatctt taccaggagt tttctgtggt 9060

gtagatgctg taaatttgct tactaacatg tttacaccac taattcaacc tattggtgct 9120

ttggacatat cagcatctat agtagctggt ggtattgtag ctatcgtagt aacatgcctt 9180

gcctactatt ttatgaggtt tagacgtgct tttggtgaat acagtcatgt agttgccttt 9240

aatactctcc tattccttat gtcattcact gtactctgtt taacaccagt ttactcattc 9300

ttacctggtg tttattctgt tatttacctg tacttgacat tttatctgac taatgatgtt 9360

tcttttctcg cacatattca gtggatggtt atgttcacac ctttagtacc tttctggata 9420

acaattgctt acatcatttg tatttccaca aagcatttct attggttctt tagtaattac 9480

ctaaagagac gtgtagtctt taatggtgtt tcctttagta cttttgaaga agctgcgctg 9540

tgcacctttt tgttaaataa ggagatgtat ctaaagttgc gtagtgatgt gctattacct 9600

cttacgcaat ataatagata cttagctctt tataacaagt acaagtattt cagtggagca 9660

atggatacaa ctagctacag agaagctgct tgttgtcatc tcgcaaaggc tctcaatgac 9720

ttcagtaact caggttctga tgttctttac caaccaccac aaacctctat cacctcagct 9780

gttttgcaga gtggttttag aaaaatggca ttcccatctg gtaaagttga gggttgtatg 9840

gtacaagtaa cttgtggtac aactacactt aacggtcttt ggcttgatga cgtagtttac 9900

tgtccaagac atgtgatctg cacctctgaa gatatgctta accctaatta tgaagatcta 9960

ctcatccgta agtctaatca taacttcttg gtacaggctg gtaatgttca actcagggtt 10020

attggacatt ctatgcaaaa ttgtgtactt aagcttaagg ttgatacagc caatcctaag 10080

acacctaagt ataagtttgt tcgcattcaa ccaggacaga ctttttcagt gttagcttgt 10140

tacaatggtt caccatctgg tgtttaccaa tgtgctatga ggcccaattt cactattaag 10200

ggttcattcc ttaatggttc atgtggtagt gttggtttta acatagatta tgactgtgtc 10260

tctttttgtt acatgcacca tatggaatta ccaactggag ttcatgctgg cacagactta 10320

gaaggtaact tttatggacc ttttgttgac aggcaaacag cacaagcagc tggtacagat 10380

acaactatta cagttaatgt tcttgcttgg ttgtacgctg ctgttataaa tggagacagg 10440

tggtttctca atcgatttac cacaactctt aatgacttta accttgtggc tatgaagtac 10500

aattatgaac ctctaacaca agaccatgtt gacatactag gacctctttc tgctcaaact 10560

ggaattgccg ttttagatat gtgtgcttca ttaaaagaac ttctgcaaaa tggtatgaat 10620

ggacgtacca tattgggtag tgctttatta gaagatgagt ttacaccttt tgatgttgtt 10680

agacaatgct caggtgttac tttccaaagt gcagtgaaaa gaacaatcaa gggtacacac 10740

cactggttgt tactcacaat tttgacttca cttttagttt tagtccagag tactcaatgg 10800

tctttgttct ttttcttcta cgaaaatgcc tttttacctt ttgctatggg tattattgct 10860

atgtctgctt ttgcaatgat gtttgtcaaa cataagcatg catttctctg tttgtttttg 10920

ttaccttctc ttgccactgt agcttacttt aatatggtct acatgcctgc tagttgggtg 10980

atgcgtatta tgacatggtt ggatatggtt gatactagtt tgtctggttt taagctaaaa 11040

gactgtgtta tgtatgcatc agctgtagtg ttactaatcc ttatgacagc aagaactgtg 11100

tatgatgatg gtgctaggag agtgtggaca cttatgaatg tcttgacact cgtttataaa 11160

gtttactatg gcaacgcttt agatcaagcc atttccatgt gggctcttat aatctctgtt 11220

acttctaact actcaggtgt agttacaact gtcatgtttt tggccagagg tattgttttt 11280

atgtgtgttg agtattgccc tattttcttc ataactggta atacacttca gtgtataatg 11340

ctagtctatt gtttcttagg ctatttttgt acttgttact tcggcctctt ttgtttactc 11400

aaccgctact ttagactgac tcttggtgtt tatgattact tagtgtctac acaggagttt 11460

agatatatga attcacaggg actactccca cccaagaata gcatagatgc cttcaaactc 11520

aacattaaat tgttgggtgt tggtggcaaa ccttgtatca aagtagccac tgtacagtct 11580

aaaatgtcag atgtaaagtg cacatcagta gtcttactct cagttttgca acaactcaga 11640

gtagaatcat catctaaatt gtgggctcaa tgtgtccagt tacacaatga cattctctta 11700

gctaaagata ctactgaagc ctttgaaaaa atggtttcac tactttctgt tttgctttcc 11760

atgcagggtg ctgtagacat aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc 11820

ttacaagcta tagcctcaga gtttagttcc cttccatcat atgcagcttt tgctactgct 11880

caagaagctt atgagcaggc tgttgctaat ggtgattctg aagttgttct taaaaagttg 11940

aagaagtctt tgaatgtggc taaatctgaa tttgaccgtg atgcagccat gcaacgtaag 12000

ttggaaaaga tggctgatca agctatgacc caaatgtata aacaggctag atctgaggac 12060

aagagggcaa aagttactag tgctatgcag acaatgcttt tcactatgct tagaaagttg 12120

gataatgatg cactcaacaa cattatcaac aatgcaagag atggttgtgt tcccttgaac 12180

ataatacctc ttacaacagc agccaaacta atggttgtca taccagacta caacacatat 12240

aagaatacgt gtgatggtac aacatttact tatgcatcag cattgtggga aatccaacag 12300

gttgtagatg cagatagtaa aattgttcag cttagtgaaa ttagtatgga caattcacct 12360

aatttagcat ggcctcttat tgtaacagct ttaagggcca attctgctgt caaattacag 12420

aataatgagc ttagtcctgt tgcactaaga caaatgtctt gtgctgccgg tactacacaa 12480

actgcttgca ctgatgacaa tgcgttagct tactacaaca caacaaaggg aggtaggttt 12540

gtacttgcac tgttatccga tttacaggat ttgaaatggg ctagattccc taagagtgat 12600

ggaactggta ctatctatac agaactggaa ccaccttgta ggtttgttac agacacacct 12660

aaaggtccta aagtgaagta tctttacttc atcaaaggat taaacaacct aaatagaggt 12720

atggtacttg gtagtttagc tgccacagta cgtttacaag ctggtaatgc aacagaagtt 12780

cctgctaatt caactgtact ttctttctgt gcttttgctg tagatgctgc taaagcttac 12840

aaagattatc tagctagtgg gggacaacca atcactaatt gtgttaagat gttgtgtaca 12900

cacactggta ctggtcaggc aataacagtt acaccggaag ccaatatgga tcaagaatcc 12960

tttggtggtg catcgtgttg tctgtactgc cgttgtcata tagatcatcc aaatcctaaa 13020

ggattttgtg acttaaaagg taagtatgta caaataccta caacttgtgc taatgaccct 13080

gtgggtttta cacttaaaaa cacagtctgt accgtctgcg gtatgtggaa aggttatggt 13140

tgtagttgtg atcaactccg cgaacccatg cttcagtcag ctgatgcaca atcgttttta 13200

aac 13203

<210> 59

<211> 8088

<212> DNA

<213> Artificial sequence

<220>

<223> SARS-CoV-2 orf1b

<400> 59

cgggtttgcg gtgtaagtgc agcccgtctt acaccgtgcg gcacaggcac tagtactgat 60

gtcgtatata gagcttttga catctacaat gataaagtag ctggttttgc taagttccta 120

aaaactaatt gttgtcgctt ccaagaaaag gacgaagatg acaatctcat tgattcttac 180

tttgtagtta agagacacac tttctctaac taccaacatg aagaaacaat ttacaacctg 240

cttaaggatt gtccagctgt tgctaaacat gacttcttta agtttagaat agacggtgac 300

atggtaccac atatatcacg tcaacgtctt actaaataca caatggcaga cctcgtctat 360

gctttaaggc attttgatga aggtaattgt gacacattaa aagaaatact tgtcacatac 420

aattgttgtg atgatgacta cttcaataaa aaggactggt atgattttgt agaaaaccca 480

gatatattac gcgtatacgc caacttaggt gaacgtgtac gccaagcttt gttaaaaaca 540

gtacagttct gtgatgccat gcgaaatgct ggtattgttg gtgtactgac attagataat 600

caagatctca atggtaactg gtatgacttt ggtgatttca tacaaaccac gccaggtagt 660

ggagttcctg ttgtagactc ttattattca ttgctcatgc ctatattaac cttgaccagg 720

gctttaactg cagagtcaca tgttgacact gacttaacaa agccttacat taagtgggat 780

ttgttaaaat acgacttcac ggaagagagg ttaaaactct ttgaccgtta ttttaaatac 840

tgggatcaga cataccaccc aaattgtgtt aactgtttgg atgacagatg cattctgcat 900

tgtgcaaact ttaatgttct gttctctaca gtgttcccac ctacaagttt tggaccacta 960

gtgagaaaaa tatttgttga tggtgttcca tttgtagttt caactggata ccacttcaga 1020

gagctaggtg ttgtacataa tcaggatgta aacttacata gctctagact tagttttaag 1080

gaattacttg tgtatgctgc tgatcctgct atgcatgctg cttctggtaa tctattacta 1140

gataaacgca ctacgtgctt ttcagtagct gcacttacta acaatgttgc ttttcaaact 1200

gtcaaacccg gtaattttaa caaggacttc tatgactttg ctgtgtctaa gggtttcttt 1260

aaggaaggaa gttctgttga attaaaacac ttcttctttg ctcaggatgg taatgctgct 1320

atcagcgatt atgactacta tcgttataat ctaccaacaa tgtgtgatat cagacaacta 1380

ctatttgtag ttgaagttgt tgataagtac tttgattgtt acgatggtgg ctgtattaat 1440

gctaaccaag tcatcgtcaa caacctagac aaatcagctg gttttccatt taataaatgg 1500

ggtaaggcta gactttatta tgattccatg agttatgagg atcaagatgc acttttcgca 1560

tatacaaaac gtaatgtcat ccctactata actcaaatga accttaagta tgccattagt 1620

gcaaagaata gagctcgcac cgtagctggt gtctctatct gtagtactat gaccaataga 1680

cagtttcatc aaaaattact caagtcaata gccgccacta gaggagctac tgtagtaatt 1740

ggaacaagca aattctatgg tggttggcac aacatgctca aaactgttta tagtgatgta 1800

gaaaaccctc accttatggg ttgggattat cctaaatgtg atagagccat gcctaacatg 1860

cttagaatta tggcctcact tgttcttgct cgcaaacata caacgtgttg tagcttgtca 1920

caccgtttct atagattagc taatgagtgt gctcaagtat tgagtgaaat ggtcatgtgt 1980

ggcggttcac tatatgttaa accaggtgga acctcatcag gagatgccac aactgcttat 2040

gctaatagtg tgtttaacat ttgtcaagct gtcacggcca atgttaatgc acttttatct 2100

actgatggta acaaaattgc cgataagtat gtccgcaatt tacaacacag actttatgag 2160

tgtctctata gaaatagaga tgttgacaca gactttgtga atgagtttta cgcatatttg 2220

cgtaaacatt tctcaatgat gatactctct gacgatgctg ttgtgtgttt caatagcact 2280

tatgcatctc aaggtctagt ggctagcata aagaacttta agtcagttct ttactatcaa 2340

aacaacgttt ttatgtctga agcaaaatgt tggactgaga ctgaccttac taaaggacct 2400

catgaatttt gctctcaaca tacaatgcta gttaaacagg gtgatgatta tgtgtacctt 2460

ccttacccag atccatcaag aatcctaggt gccggttgtt ttgtagatga tatcgtaaaa 2520

acagatggta cacttatgat tgaacggttc gtgtctttag ctatagatgc ttacccactt 2580

actaaacatc ctaatcagga gtatgctgat gtctttcatt tgtacttaca atacatacgt 2640

aagctacatg atgagttaac aggacacatg ttagacatgt attctgttat gcttactaat 2700

gataacactt caaggtattg ggaacctgag ttttatgagg ctatgtacac accgcataca 2760

gtcttacaag ctgttggtgc ttgtgttctt tgcaattcac agacttcatt aagatgtggt 2820

gcttgcatac gtagaccatt cttatgttgt aaatgctgtt acgaccatgt catctcaaca 2880

tcacataaat tagtcttgtc tgttaatccg tatgtttgca atgctccagg ttgtgatgtc 2940

acagatgtga ctcaacttta cttaggaggt atgagctatt actgtaagtc acataaacca 3000

cccattagtt ttccattgtg tgctaatgga caagtttttg gtctctacaa gaatacatgt 3060

gttggtagcg ataatgttac tgactttaat gcaattgcaa catgtgactg gacaaatgct 3120

ggtgattaca ttttagctaa cacctgtact gaaagactca agctttttgc agcagaaacg 3180

ctcaaagcta ctgaggagac atttaaactg tcttatggta ttgctactgt acgtgaagtg 3240

ctgtctgaca gagaattaca tctttcatgg gaagttggta aacctagacc accacttaac 3300

cgaaattatg tctttactgg ttatcgtgta actaaaaaca gtaaagtgca aatcggagag 3360

tacacctttg aaaaaggtga ctatggtgat gctgttgttt accgaggtac aacaacttac 3420

aaactcaacg ttggtgatta ttttgtgctg acatcacata cagtaatgcc attaagtgca 3480

cctacactag tgccacaaga gcactatgtt agaattactg gcttataccc aacactcaat 3540

atctcagatg agttttctag caatgttgca aattatcaaa aggttggtat gcaaaagtat 3600

tctacactcc agggaccacc tggtactggt aaaagtcatt ttgctattgg tctagctctc 3660

tactaccctt ctgctcgcat agtatataca gcttgctctc atgcagctgt tgatgcacta 3720

tgtgagaagg cattaaaata tttgcccata gacaaatgta gtagaattat acctgcacgt 3780

gctcgtgtag agtgttttga taaattcaag gtgaattcaa cattagaaca gtatgtcttt 3840

tgtactgtaa atgcattgcc tgagacgaca gcagatatag ttgtctttga tgaaatttca 3900

atggccacaa attatgattt gagtgttgtc aatgccagat tacgtgctaa gcactatgtg 3960

tacattggtg atcctgctca attacctgca ccacgcacat tactaactaa gggtacacta 4020

gaaccagaat atttcaattc agtgtgtaga cttatgaaaa ctataggtcc agacatgttc 4080

ctcggaactt gtcgtagatg tcctgctgaa attgttgaca ctgtgagtgc tttggtttat 4140

gataataagc ttaaggcaca taaagacaaa tcagctcaat gctttaaaat gttctacaag 4200

ggtgttatca cgcatgatgt ttcatctgca attaacaggc cacaaatagg cgtggtaaga 4260

gaattcctta cacgtaaccc tgcttggaga aaagctgtct ttatttcacc ttacaattcc 4320

cagaatgctg tagcctcaaa gattttggga ctaccaactc aaactgttga ttcatcacag 4380

ggctcagaat atgactatgt catattcact caaaccactg aaacagctca ctcttgtaat 4440

gtaaacagat tcaacgttgc tattaccaga gcaaaagtag gcatactttg cataatgtct 4500

gatagagacc tttatgacaa gttgcaattt acaagtcttg aaattccacg taggaatgtg 4560

gcaactttac aagctgaaaa tgtaacagga ctctttaaag attgtagtaa ggtaatcact 4620

gggttacatc ctacacaggc acctacacac ttaagtgttg atactaaatt caaaactgaa 4680

ggtttatgtg ttgacatacc tggcatacct aaggacatga cctatagaag attaatctct 4740

atgatgggtt tcaaaatgaa ttaccaggtt aatggttacc ctaacatgtt tatcacccgc 4800

gaagaagcta taagacatgt acgtgcatgg attggcttcg atgtcgaagg ttgtcatgct 4860

actagagaag ctgttggtac caatttacct ttacagctag gtttttctac aggtgttaac 4920

ctagttgctg tacctacagg ttatgttgat acacctaata atacagattt ttccagagtt 4980

agtgctaaac caccgcctgg agatcaattt aaacacctca taccacttat gtacaaagga 5040

cttccttgga atgtagtgcg tataaagatt gtccaaatgt taagtgacac acttaaaaat 5100

ctctctgaca gagtcgtatt tgtcttatgg gcacatggct ttgagttgac atctatgaag 5160

tattttgtga agatcggacc tgagcgcaca tgttgtctat gtgatagacg tgctacatgc 5220

ttttccactg cttcagacac ttatgcctgt tggcatcatt ctattggatt tgattacgtc 5280

tataatccgt ttatgattga tgttcaacaa tggggtttta caggtaacct acaaagcaac 5340

catgatctgt attgtcaagt ccatggtaat gcacatgtag ctagttgtga tgcaatcatg 5400

actaggtgtc tagctgtcca cgagtgcttt gttaagcgtg ttgactggac tattgaatat 5460

cctataatcg gtgatgaact gaagattaat gcggcttgta gaaaggttca acacatggtt 5520

gttaaagctg cattattagc agacaaattc ccagttcttc acgacattgg taaccctaaa 5580

gctattaagt gtgtacctca agctgatgta gaatggaagt tctatgatgc acagccttgt 5640

agtgacaaag cttacaaaat agaagaactg ttctattctt atgccacaca ttctgacaaa 5700

ttcacagatg gtgtatgcct attttggaat tgcaatgtcg atagatatcc tgctaattcc 5760

attgtttgta gatttgacac tagagtgcta tctaacctta acttgcctgg ttgtgatggt 5820

ggcagtttgt atgtaaataa gcatgcattc cacacaccag cttttgataa aagtgctttt 5880

gttaatctaa agcaacttcc atttttctat tactctgaca gtccatgtga gtctcatgga 5940

aaacaagtag tgtcagatat agattatgta ccactaaagt ctgctacgtg tataacacgt 6000

tgcaatttag gtggtgctgt ctgtagacat catgctaatg agtacagatt gtatctcgat 6060

gcttataaca tgatgatctc agctggcttt agcttgtggg tttacaaaca atttgatacc 6120

tataacctct ggaacacttt tacaagactt cagagtttag aaaatgtggc ttttaatgtt 6180

gtaaataagg gacactttga tggacaacag ggtgaagtac cagtttctat cattaacaac 6240

actgtttaca caaaagttga tggtgttgat gtagaattgt ttgagaacaa aaccacatta 6300

cctgttaatg tagcatttga gctttgggct aagcgcaaca ttaaaccagt accagaggtg 6360

aaaatactca ataatttggg tgtggacatt gctgctaata ctgtgatctg ggactacaaa 6420

agagatgctc cagcacatat atctactatt ggtgtttgtt ctatgactga catagccaag 6480

aaaccaactg aaacgatttg tgcaccactc actgtctttt ttgatggtag agttgatggt 6540

caagtagact tatttagaaa tgcccgtaat ggtgttctta ttacagaagg tagtgttaaa 6600

ggtttacaac catctgtagg tcccaaacaa gctagtctta atggagtcac attaattgga 6660

gaagccgtaa aaacacagtt caattattac aagaaagtgg atggtgttgt ccaacaatta 6720

cctgaaactt actttactca gagtagaaac ttacaggaat ttaagcccag gagtcaaatg 6780

gaaattgatt tcttagaact tgctatggat gaattcattg aacggtataa attagaaggc 6840

tatgccttcg aacatatcgt ttatggagat tttagtcata gtcagttagg tggtttacat 6900

ctactgattg gactagctaa acgttttaag gaatcacctt ttgaacttga agattttatt 6960

cctatggaca gtacagttaa aaactacttc ataacagatg cgcaaacagg ttcatctaag 7020

tgtgtgtgtt ctgttattga tcttttactt gatgacttcg ttgaaataat aaagtcccaa 7080

gatttatctg tagtttctaa ggttgtcaaa gtgactattg actatacaga aatctcattt 7140

atgctttggt gtaaagatgg ccatgtagaa acattttacc caaaattaca atctagtcaa 7200

gcgtggcaac cgggtgttgc tatgcctaat ctttacaaaa tgcaaagaat gctattagaa 7260

aagtgtgacc ttcaaaatta tggtgatagt gcaacattac ctaaaggcat aatgatgaat 7320

gtcgcaaaat atactcaact gtgtcaatat ttaaacacac tgacattagc tgtaccctat 7380

aatatgagag ttatccattt tggtgctggt tctgataaag gagttgcacc aggtacagct 7440

gttttaagac aatggttgcc tacaggtacg ctgcttgtcg attcagatct taatgacttt 7500

gtctctgatg cagattcaac tttgattggt gattgtgcaa ctgtacatac agctaataaa 7560

tgggatctca ttattagtga tatgtacgac cctaagacta agaatgtcac aaaagaaaac 7620

gactctaaag agggtttttt cacttacatt tgtgggttta tacaacaaaa gctagctctt 7680

ggaggttccg tggctataaa gataacagaa cattcttgga atgctgatct ttataagctc 7740

atgggacact tcgcatggtg gacagccttt gttactaatg tgaatgcgtc atcatctgaa 7800

gcatttttaa tcggatgtaa ctaccttggc aaaccacgcg aacaaataga tggttatgtc 7860

atgcatgcaa attacatatt ttggaggaat acaaatccaa ttcagctttc ttcttattct 7920

ttattcgaca tgagtaaatt cccccttaaa ttaaggggta ctgctgttat gtctttaaaa 7980

gaaggtcaaa tcaatgatat gattctctct cttcttagta aaggtagact tataattaga 8040

gaaaacaaca gagttgttat ttctagtgat gttcttgtta acaactaa 8088

Claims

1. A fully synthetic long-chain nucleic acid having at least 4,000 bases, characterized in that said nucleic acid comprises at least two of the four sequence portions A-D in any arrangement, wherein

i) Sequence part A comprises

b) The sequence defined in seq.id.3;

ii) sequence part B comprises

b) The sequence defined in seq.id.7;

iii) Sequence part C comprises

b) The sequence defined in seq.id.11;

Covering ribonucleic acid sequences corresponding to deoxyribonucleic acid sequences according to the sequence parts a-D.

2. Nucleic acid according to claim 1, characterised in that it has at least 8'000 bases, preferably at least 20'000 bases in the defined sequence.

3. The nucleic acid of any one of the preceding claims, wherein the nucleic acid further comprises

a) 1.) an ORF1ab sequence defined by seq.id.51 or a sequence having at least 98.5% sequence identity to seq.id.51; or

ii) the ORF1a sequence defined by seq.id.58 or a sequence having at least 98.6% sequence identity to seq.id.58;

c) The ORF7a sequence defined by seq.id.54 or a sequence having at least 99.5% sequence identity to seq.id.54.

4. The nucleic acid of claim 3, wherein the nucleic acid further comprises

5. Nucleic acid according to any one of the preceding claims, characterized in that the sequence portions a to C correspond to the sequence according to seq.id.19 or the corresponding ribonucleic acid sequence.

6. Nucleic acid according to any one of the preceding claims, characterized in that the nucleic acid comprises at least three of the four sequence portions a-D in any arrangement or at least three of the four sequence portions having a ribonucleic acid sequence corresponding to the deoxyribonucleic acid sequence according to the sequence portions a-D.

7. Nucleic acid according to any one of the preceding claims, characterized in that it comprises, in any arrangement, said four sequence portions a-D or four sequence portions having a ribonucleic acid sequence corresponding to the deoxyribonucleic acid sequence according to said sequence portions a-D.

8. Nucleic acid according to any one of the preceding claims, characterized in that it further comprises at least one of the following sequences:

sequence consisting of seq.id.15

Sequence consisting of seq.id.28

Sequence consisting of seq id.29 and

a sequence consisting of SEQ ID.30,

or comprises one of the deoxyribonucleic acid sequences according to the sequence parts seq.id.15, seq.id.28, seq.id.29 and seq.id.30 or the corresponding ribonucleic acid sequence.

9. Nucleic acid according to any one of the preceding claims, characterised in that it has a maximum size of 1'000 bases, preferably of 200'000 bases.

10. A vector comprising a nucleic acid according to any preceding claim.

11. The vector of claim 10, wherein the vector comprises the sequences defined in seq.id.46 and seq.id.47.

12. The vector according to any one of claims 10 to 11, wherein the vector is a plasmid vector.

13. A kit comprising two or more nucleic acids according to any one of claims 1 to 9.

14. The kit according to claim 13, wherein the nucleic acid is present in at least one plasmid, preferably in two or more plasmids.

15. A biotechnological production unit comprising at least one vector according to claims 10 to 12.

16. A viral envelope, a viral envelope fragment and/or a viral envelope protein obtainable by gene expression using at least one nucleic acid according to any one of claims 1 to 9, using a vector according to any one of claims 10 to 12, using a kit according to any one of claims 13 or 14, or a biotechnological production unit according to claim 15, wherein the viral envelope, the viral envelope fragment and/or the viral envelope protein packages at least one nucleic acid according to any one of claims 1 to 9.

17. Vaccine against coronavirus SARS-CoV-2, comprising at least one nucleic acid according to any of claims 1 to 9 and a product obtainable by gene expression in a production organism using at least one nucleic acid according to any of claims 1 to 9, using a vector according to any of claims 10 to 12, using a kit according to any of claims 13 or 14, in particular comprising a virus envelope, a virus envelope fragment and/or a virus envelope protein according to claim 16.

18. The vaccine according to claim 17, comprising at least two molecularly precisely defined protein components selected from protein components a, b1, b2, c1, c2, d1 or d2, wherein

(i) The protein component a comprises

(ii) The protein component b1 comprises

a) A sequence according to seq.id.6 or a sequence having at least 90% sequence identity to seq.id.6 that is similar to envelope protein E of SARS-CoV-2; or

b) A sequence according to seq.id.21 or a sequence having at least 90% sequence identity to seq.id.21 similar to envelope protein E of SARS-CoV-2; and

the protein component b2 comprises a sequence according to seq.id.8 similar to envelope protein E of MHV59A, or an equivalent protein comprising a sequence having at least 90% sequence identity to seq.id.8;

(iii) The protein component c1 comprises

the protein component c2 comprises a sequence according to seq.id.12 similar to MHV59A membrane protein M, or an equivalent protein comprising a sequence having at least 90% sequence identity to seq.id.12; and

(iv) The protein component d1 comprises

the protein component d2 comprises a sequence according to seq.id.4 similar to the nucleocapsid phosphoprotein N of MHV59A or an equivalent protein comprising a sequence having at least 90% sequence identity to seq.id.no. 4.

a) Introducing a nucleotide sequence according to any of claims 1 to 9 into a biotechnological production unit, in particular a cell line,

b) Obtaining a protein component from the biotechnological production unit in the step a); and

c) Purifying the obtained protein component to obtain the vaccine against coronavirus SARS-CoV-2.

20. A method for producing a vaccine against coronavirus SARS-CoV-2, said vaccine comprising a viral envelope, a viral envelope fragment and/or a viral envelope protein according to claim 16, said method comprising the following successive steps:

a) Introducing a nucleotide sequence according to any of claims 1 to 9 into a biotechnological production unit, wherein the biotechnological production unit comprises nucleotides encoding at least one protein component selected from the group consisting of protein components a, b1, c1 and d 1;

b) Obtaining a viral envelope fragment and/or a viral envelope protein from said biotechnological production unit in said step a); and

c) Purifying the obtained protein component to obtain the vaccine against coronavirus SARS-CoV-2, said vaccine comprising a viral envelope, a viral envelope fragment and/or a viral envelope protein according to claim 16.

a) Introducing a vector according to any one of claims 10 to 12 into an amplification biotechnological production unit;

b) Amplifying the nucleotide of any one of claims 1 to 9 in the amplification biotechnological production unit;

c) Obtaining the amplified nucleotides of step b);

d) The vaccine against coronavirus SARS-CoV-2 is obtained by using the method according to claim 19 or 20.