CN115739977A - Application of paenibacillus kribbensis space mutation strain in repairing Cd pollution - Google Patents
Application of paenibacillus kribbensis space mutation strain in repairing Cd pollution Download PDFInfo
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an application of a paenibacillus kribbensis space mutation strain in repairing Cd pollution. According to the invention, the mutant delta PS04-17 strain obtained by aerospace mutagenesis is researched, and the delta PS04-17 strain has stronger cadmium resistance than a wild-type PS04 strain, can well grow in a cadmium-containing culture medium of 300mg/L, and even can tolerate the concentration of up to 800 mg/L. The delta PS04-17 can relieve cadmium stress of rice and promote the growth of the rice under the cadmium stress; meanwhile, the delta PS04-17 can also obviously reduce the cadmium content and has a fixing effect on cadmium ions; after the delta PS04-17 is adopted to treat the soil, the concentration of soluble cadmium in the soil can be obviously reduced. The invention shows that the delta PS04-17 can be used for treating cadmium pollution of water and soil, can relieve cadmium stress on plants, promotes the growth of the plants under the cadmium stress, and provides an important strain resource for microbial remediation of cadmium pollution.
Description
Technical Field
The invention belongs to the technical field of agricultural microorganisms. More particularly, relates to an application of a paenibacillus kribbensis space mutation strain in repairing Cd pollution.
Background
The self-circulation capability of the soil is greatly influenced by the pollution of heavy metals. Relevant research shows that in the development of agricultural economy in China, soil heavy metal pollution becomes one of the main limiting factors, the total soil heavy metal pollution area in China exceeds 5000 ten thousand mu, and the numerical value is increased year by year. The heavy metal pollution of the soil is difficult to treat, the concealment is strong, and the normal production and the life of human are not facilitated. From the current development situation, the problem of heavy metal pollution of soil in China is still in a gradually aggravated state. After the heavy metal enters the soil, the growth and development of crops can be directly influenced, so that the yield and the quality greatly slide down. For example, cadmium is a great hazard in plant growth, and if the content of cadmium in soil is too high, chlorophyll structures in plant leaves can be damaged, so that water absorption of plant root systems is influenced, and plant growth is inhibited. More importantly, these heavy metal elements enter the human body through the food chain. For example, cadmium is highly toxic and is likely to cause bone pain, cardiovascular and cerebrovascular diseases, hypertension, and other diseases.
After the content of heavy metal elements in the soil reaches a certain state, the metabolism, the survival and the like of microorganisms are greatly influenced, the specific expression is that the integral quantity of the microorganisms is reduced, the action of active bacteria cannot be exerted, the ratio of the generated biomass carbon to the organic carbon also can slide down greatly, the respiration action is weakened, and the function and the structure of the microorganisms in the soil are obviously changed. For example, relevant studies clearly show that cadmium has a great influence on the activity of soil organisms and enzymes, and especially has the most significant influence on the activity of urease. More importantly, under the influence of heavy metal pollution, the water quantity of microorganisms in the soil can be reduced, the community composition of the microorganisms in the soil can be changed, the proportion of common dominant communities is reduced, and the original diversity and uniformity indexes cannot be displayed. Under the action of heavy metal pollution, the soil can not keep the original appearance, thereby bringing adverse factors to the growth of various plants and influencing the benefits of planting personnel.
The current measures for treating heavy metal pollution of soil mainly comprise the following measures: (1) The physical method mainly comprises an electrochemical method, a thermal desorption method and a vitrification technology. Most of the physical methods have excellent test effects in indoor environments, but have poor applicability in outdoor environments; the equipment has higher operation cost and more electric energy consumption, and is easy to cause pollution during recovery. (2) The chemical method mainly comprises the steps of putting a soil conditioner, leaching and pumping out. The chemical method has certain effect and low treatment cost, but is easy to cause pollution and has the possibility of causing pollution transfer. (3) The biological method mainly comprises microbial repair and phytoremediation. The soil is rich in various microorganisms, and part of the bacteria, fungi and other substances can fully exert the effects of adsorption, oxidation and the like on heavy metal pollutants, so that the pollution toxicity is reduced. The cell wall is used as the middle part of the bacteria and the heavy metal in the soil, phosphate radical ions and carboxyl anions are more, and active cations in the soil are easily combined on the surface layer. Other metabolites of bacteria can play a strong activating role in partial metal ions in a dissolved state, realize the fixed adsorption of soil heavy metals and have strong soil remediation capability. The plant solidifying technology is not a soil heavy metal removing technology, and only attracts and fixes heavy metal pollutants in the soil to the roots and rhizosphere of plants. In the practical operation of the plant restoration technology, in order to ensure the high efficiency of the restoration work, workers also need to track and monitor the soil in the area for a long time.
The existing microbial strains capable of repairing soil heavy metal pollution are Pseudomonas (Pseudomonas sp.), enterobacter (Enterobacter sp.), serratia marcescens (Serratia marcescens), chinese Pseudomonas (Sinomonas flava), acidithiobacillus (Chaetomium globosum), agrobacterium (Agrobacterium sp.) and the like, wherein the strains capable of being used for repairing soil heavy metal cadmium are few. The patent CN114480222A discloses a Paenibacillus kribbensis space mutant delta PS04-17, the antibacterial effect of which is obviously superior to that of a wild-type PS04 strain, the Paenibacillus kribbensis space mutant delta PS04-17 has broader antibacterial activity and stronger growth activity than that of the wild-type PS04 strain, and also has better saline-alkali tolerance than that of the wild-type strain, can relieve saline-alkali stress of tomatoes and promote the tomatoes to grow under the saline-alkali stress; however, the effect of the paenibacillus kribbensis space mutant delta PS04-17 in heavy metal cadmium repair is not reported at present.
Disclosure of Invention
The invention provides a new application of a Paenibacillus kribbensis (Paenibacillus kribbensis) space mutation mutant strain delta PS04-17 in repairing cadmium pollution.
The invention also aims to provide a method for fixing cadmium ions in the solution and relieving the cadmium stress of plants or repairing the cadmium pollution of soil.
The above purpose of the invention is realized by the following technical scheme:
the invention provides a new application of a Paenibacillus kribbensis space mutant delta PS04-17 in repairing cadmium pollution, wherein the adopted delta PS04-17 strain is obtained by space condition mutagenesis. The invention shows that the delta PS04-17 strain has stronger cadmium resistance than the wild PS04 strain, the wild PS04 strain is obviously inhibited from growing in a 50mg/L cadmium-containing flat plate, and only a small number of colonies grow; the delta PS04-17 strain can still grow normally, and the number of colonies is large; in a culture medium containing high-concentration cadmium, the wild-type PS04 can not grow at all, and the delta PS04-17 strain can grow under the condition of 300mg/L, which shows that the cadmium resistance of the mutant strain delta PS04-17 is obviously improved. Meanwhile, the delta PS04-17 strain has a growth promoting effect on the plant, and can remarkably relieve the inhibiting effect of cadmium stress on the growth of the plant; the delta PS04-17 strain can obviously reduce the content of cadmium ions in the solution and has a fixing effect on the cadmium ions; the delta PS04-17 strain can also reduce the concentration of soluble cadmium in soil. On the whole, after space mutation of Paenibacillus kribbensis, a mutant strain delta PS04-17 obtained by screening has obvious forward variation in growth capacity and cadmium resistance, and is a strain with excellent mutation characteristics.
Therefore, the invention provides the application of the space mutant delta PS04-17 strain or the bacterial liquid thereof in adsorbing or passivating cadmium ions, preparing a cadmium ion passivator and/or a microbial inoculum for reducing the cadmium content, relieving cadmium stress of plants and/or promoting plant growth under the cadmium stress, repairing cadmium pollution of soil and preparing a soil cadmium pollution repairing agent.
Furthermore, the Paenibacillus kribbensis space mutation mutant strain delta PS04-17 strain is preserved in the Guangdong province microorganism strain preservation center at 2022, 3 and 17 days, the preservation number is GDMCC NO:62233, and the preservation address is No. 59 building 5 of the great institute of Miehu 100 of Guangdong province.
The invention provides a method for fixing cadmium ions in a solution, which adopts a paenibacillus kribbensis space mutation mutant strain delta PS04-17 bacterial solution and a sample solution for co-culture treatment.
The invention provides a method for relieving cadmium stress of plants or repairing cadmium pollution of soil, which adopts bacillus kribbensis space mutation mutant strain delta PS04-17 bacterial liquid to treat roots of plants or soil.
Preferably, the bacterial liquid is fermentation liquid.
More preferably, the fermentation broth is a fermentation supernatant.
In particular, the person skilled in the art can prepare Δ PS04-17 fermentation broth according to various methods disclosed in the literature; for example, a secondary culture method is employed, namely: seed culture and liquid fermentation culture to prepare the fermentation liquor. In the liquid fermentation culture, fermentation can be performed by adopting a fermentation tank or by adopting a shake flask culture mode. Wherein the components of the liquid fermentation medium comprise a carbon source and a nitrogen source; the carbon source is sucrose or soluble starch. The nitrogen source includes, but is not limited to, any one or more of sodium nitrate, ammonium chloride or peptone.
Preferably, the broth dilution is used 100-fold.
Preferably, the fermentation broth OD = 1.0-2.0, the concentration of the fermentation broth is 1-10%, and the fermentation conditions are as follows: inoculating the delta PS04-17 bacterial liquid into a culture medium, fermenting for 3-4 days at 25-30 ℃ and 150-200 rpm.
The invention has the following beneficial effects:
the invention provides a new application of a paenibacillus kribbensis space mutant delta PS04-17 strain in the aspect of repairing cadmium pollution, and the research of the invention shows that the delta PS04-17 strain has stronger cadmium resistance and can grow well in a culture medium containing high-concentration cadmium; meanwhile, the delta PS04-17 strain can relieve cadmium stress of rice and promote the growth of the rice under the cadmium stress; the delta PS04-17 strain can also obviously reduce the content of cadmium ions in the solution and has a fixing effect on the cadmium ions; and the concentration of soluble cadmium in the soil can be reduced, and the cadmium-removing agent is used for relieving the stress of cadmium plants or repairing the soluble cadmium in the soil. According to the invention, through research on the mutant strain of Paenibacillus kribbensis, the tolerance of the strain delta PS04-17 to cadmium is obviously improved, cadmium ions can be fixed and passivated very low, the strain can be used for repairing the cadmium pollution of heavy metal in the environment, the cadmium-polluted soil is treated, the cadmium stress on plants can be eliminated, the growth of the plants is promoted, and the important application significance is realized on the repair and utilization of the cadmium-polluted soil.
Drawings
FIG. 1 is a diagram showing the growth morphology of the strain on LB plates (the left side shows the colony morphology of PS04, and the right side shows the colony morphology of. DELTA.PS 04-17);
FIG. 2 is a diagram showing the growth state of a strain in a Czochralski liquid medium (the first form from the left is PS04, the second form from the left is. DELTA.PS 04-17, and the third form from the left is. DELTA.PS 04-17 cultured in a cadmium-containing medium);
FIG. 3 is a morphology of the strain under an electron microscope (the first from the left is the morphology of PS04 under an electron microscope, the second from the left is the morphology of Δ PS04-17 under an electron microscope, and the third from the left is the morphology of Δ PS04-17 under an electron microscope cultured in a cadmium-containing medium);
FIG. 4 is a graph showing the growth of the strain Δ PS04-17 on a cadmium-containing medium with a concentration gradient (upper: under the condition of Δ PS 04-17: PS04; the concentrations from left to right are respectively 50mg/L, 100mg/L, 200mg/L, 400mg/L, 800 mg/L);
FIG. 5 is a graph showing the growth of the strain in a Czochralski liquid medium;
FIG. 6 shows that the strain contains 300mg/L CdCl 2 Growth profile in czochralski broth;
FIG. 7 is a diagram showing the experimental results of a cadmium toxicity-resistant rice pot culture (from left to right, respectively: A: sterile water is added only to the control group 1, B: delta PS04-17 bacterial liquid and sterile water are added to the treatment group 1, and C: cdCl is added to the treatment group 2) 2 + sterile water, D: treatment group 3 addition of CdCl 2 Solution + delta PS04-17 bacteria solution + sterile water);
FIG. 8 shows the effect of the Δ PS04-17 strain on the treatment of cadmium ions in solution;
FIG. 9 shows the effect of adding Δ PS04-17 diluted bacterial liquid on the passivation of water-soluble cadmium in dry soil (CK is clear water control, and 0.1, 1, 10, 100, 200mL of diluted bacterial liquid with 100 is added for 1, 2, 3, 4, 5, respectively).
Detailed Description
The invention is further described with reference to the drawings and the following detailed description, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
The media used in the following examples were:
LB solid Medium (g/L): tryptone 10, yeast extract 5, sodium chloride 5 and agar powder 17.
Modified Chao's liquid medium (g/L): 20.0 parts of sodium nitrate, 0.7 part of monopotassium phosphate, 0.3 part of dipotassium phosphate, 0.5 part of potassium chloride, 0.5 part of magnesium sulfate, 0.01 part of ferrous sulfate and 30.0 parts of cane sugar, and the pH value is 7.0-7.2.
The space mutation mutant strain delta PS04-17 of the Paenibacillus kribbensis (Paenibacillus kribbensis) is preserved in the Guangdong province microorganism strain preservation center at 2022, 3 and 17 days, and the preservation numbers are as follows: GDMCCNO 62233, application publication number: CN 114480222A.
Example 1 comparison of cadmium resistance
The mutant strains adopted by the invention are obtained by screening from a mutant library, 30 mutant strains are obtained by primary screening in the mutant library, and the cadmium resistance of the mutant strains is compared. Respectively inoculating 30 mutant strains in an LB liquid culture medium, culturing for 48h at 28 ℃ under the environment of 200r/min to obtain a fermentation liquid, adding 50% of glycerol, and preserving the strains at low temperature for later use. Taking Paenibacillus kribbensis (Paenibacillus kribbensis) wild strain PS04 as a reference, respectively inoculating the wild strain and 30 mutant strains obtained by primary screening to the strain containing Cd by using a sterilized toothpick according to the numbering sequence (mutants 1-30) 2+ (300 mg/L) and cultured at 28 ℃ for 48 hours, and the growth of the wild type colonies was compared with that of the mutant colonies.
The result shows that the wild type strain PS04 of the Paenibacillus kribbensis contains 300mg/L CdCl 2 Cannot grow on the plate; while the mutant is CdCl at 300mg/L 2 The growth of the plates was: mutants No. 17 and 20 had the strongest growth ability, and the others were relatively weak. The growth of some of the aerospace mutant strains on cadmium-containing plates is shown in Table 1, and it was found that mutant 17 (i.e., the. DELTA.PS 04-17 strain, hereinafter collectively referred to as. DELTA.PS 04-17 strain) has better cadmium tolerance than other mutants and wild-type strains.
TABLE 1 growth of a subset of space mutagenized strains on drug-containing plates
Note: "-" indicates no growth; "+" indicates growth ability
Example 2 growth of the Strain Δ PS04-17 in a Medium containing septa
1. Comparison of Strain morphology
Growth morphology on LB plates: and (3) coating 100 mu L of bacterial liquid on an LB plate, inverting the plate after coating, and culturing in an incubator at 28 ℃ for 48h and observing.
Growth morphology in czochralski liquid medium: 100mL of Chao's liquid medium was taken, inoculated loops were used to scrape the cells from PS04,. DELTA.PS 04-17 and plates containing Cd (300 mg/L). DELTA.PS 04-17, and the cells were cultured in a shaker at 28 ℃.
2. Morphology of cells under electron microscope
And (4) collecting a sample by using single cells, and centrifugally collecting the size of the soybeans. Using a 1.5mL centrifuge tube, the culture medium was centrifuged gently and briefly, the cells were collected, fresh buffer was added, the mixture was centrifuged at 5000rpm/min for 5min, and the supernatant was discarded. Fresh phosphate buffer is dripped to resuspend for 15min, the temperature is low at 5000rpm/min, the mixture is centrifuged for 5min, the supernatant is discarded, and the process is repeated for 2 times. Adding glutaraldehyde fixing solution into the sample, then resuspending, fixing overnight at 4 ℃, and then carrying out electron microscope scanning. And observing the shapes of the wild type and the mutant under an electron microscope.
3. Results of the experiment
As shown in FIG. 1, the mutant strain Δ PS04-17 is compared with the wild-type PS04 strain and the form thereof on LB plates, and the surface of the PS04 colony is covered with a large amount of polysaccharides and the colony is more viscous than the Δ PS 04-17.
In the growth state in the czochralski liquid medium, as shown in fig. 2, it was observed that the PS04 bacterial liquid was slightly white and the bacterial liquid had a large viscosity; the delta PS04-17 bacterial liquid is yellow, and the viscosity of the bacterial liquid is smaller than that of PS04; the bacterium liquid of delta PS04-17+ Cd is slightly transparent, and the bacterium liquid viscosity is smaller than that of the original bacterium liquid of delta PS 04-17.
The cell morphology under electron microscope is shown in FIG. 3, and observation of the morphological sizes of PS04 and Δ PS04-17 by electron microscope revealed that Δ PS04-17 cells (1.04-3.33 μm 0.29-0.64 μm) were shorter than PS04 cells (2.59-6.72 μm 0.34-0.50 μm), while Δ PS04-17 cells (0.68-1.84 μm 0.29-0.47 μm) cultured in a Cd medium containing 300mg/L were shorter than the natural morphology of Δ PS04-17 cells (1.04-3.33 μm 0.29-0.64 μm).
EXAMPLE 3 determination of the cadmium resistance of the Δ PS04-17 Strain
1. Experimental methods
The preparation method of the cadmium-containing Czech solid gradient culture medium comprises the following steps: 3.5g of liquid Grignard reagent powder and 1.7g of agar were added, and 100mL of deionized water was added thereto for sterilization. CdCl with configuration of 10g/L 2 10mL of solution, cdCl should be added according to the calculation of required concentration 2 The volume of solution was added to the medium and mixed well, pouring 15mL of solution per plate.
Screening the concentration of cadmium resistance of the strain: and (3) plating and activating the wild type and mutant strains, and picking out single colony shake bacteria 2d. Adding 1mL of the bacterial liquid into 100mL of improved Chaudou culture medium, and performing shaking culture for 3d to obtain the bacterial liquid. Measuring absorbance of the bacterial liquid, adding improved Chao's culture medium, and diluting to OD 600 ＝1.0。
CdCl with different concentrations is added into each 5mL improved Czochralski plate according to a certain gradient 2 The solutions were cultured at 28 ℃ by applying 50. Mu.L of each of the wild type and mutant strain solutions (prepared as described above) to an improved Kirchner plate (set at 5 concentrations of 50, 100, 200, 400, and 800 mg/L), and observing the growth.
The strain is CdCl at 300mg/L 2 Drawing a growth curve under the solution concentration: 1mL of mutant fermentation liquor is taken and dripped with CdCl with the concentration of 300mg/L 2 The wild-type PS04 strain was treated in the same manner as in the solution-modified Chaudou medium (100 mL). So as not to add CdCl 2 The culture medium (2) is blank, and OD is measured by a spectrophotometer at 30 ℃ and 180r/min intervals of 48h 600 And (4) taking the value and drawing an OD value growth curve graph.
2. Results of the experiment
The growth of the strain on the cadmium-containing gradient plate is shown in FIG. 4, the wild-type PS04 can only grow sporadic colonies in 50mg/L cadmium-containing Cnahs medium, and the delta PS04-17 colonies grow more colonies; in 800mg/L Czochralski medium containing cadmium, wild-type PS04 hardly grows, and the number of delta PS04-17 colonies is reduced, but still grows.
The growth curve of the strain in liquid medium is shown in FIG. 5, and the result shows that the OD value of the mutant strain delta PS04-17 is increased faster than that of the wild strain, which indicates that the delta PS04-17 growsThe long activity is stronger than that of the wild type. The strain is CdCl at 300mg/L 2 The lower growth curve is shown in FIG. 6, in the presence of CdCl at 300mg/L 2 The OD values of the wild-type strain grown in the medium of (1) were in a flat line, indicating that the wild-type PS04 strain could not grow at this concentration, whereas the. DELTA.PS 04-17 strain increased exponentially at a concentration of 300 mg/L.
Example 4 ability of Δ PS04-17 Strain to repair soil cadmium stress
1. Experimental methods
And (3) evaluating the cadmium resistance of the fermentation liquor of the mutant strain delta PS04-17 to rice by using a pot experiment. According to the experimental result of example 3, 50mg/L is determined as the concentration of cadmium stress, rice seeds are bred to 2-3 cm and planted in 400g of soil, and grouping experiments are carried out. The experiment is divided into: a: control (100 mL sterile water only), B: treatment group 1 (added with 20mL of 100-fold diluted OD =1.0 Δ PS04-17 bacterial solution +80mL of sterile water), C: treatment group 2 (with addition of 25mg CdCl) 2 +100mL sterile water), treatment D group 3 (with 25mg CdCl addition) 2 Solution +20mL OD =1.0 Δ PS04-17 bacterial fluid diluted 100 times +80mL sterile water). And (3) supplementing 20mL of sterile water to each group of rice seeds every 48h, ensuring that cadmium stress is maintained at 50mg/L, observing the growth condition of the plants after culturing for 14d, and recording data.
2. Results of the experiment
The experimental result of cadmium toxicity in potted rice is shown in figure 7, the growth vigor of the rice added with the delta PS04-17 in the group B is stronger than that of the control group A, and the result shows that the delta PS04-17 bacterial liquid has a certain growth promoting effect on rice plants; group C added 50mg/LCdCl 2 The solution obviously inhibits the growth of rice; group D added 50mg/L CdCl 2 The growth vigor of the rice is obviously stronger than that of the group C and is close to that of the group A, and the solution and the delta PS04-17 bacterial solution show that the delta PS04-17 bacterial solution can greatly enhance the cadmium resistance of rice plants.
Example 5 immobilization of cadmium ions by the Δ PS04-17 Strain
The preparation method of the cadmium-containing Czochralski liquid culture medium comprises the following steps: 35g of the Czochralski medium was added to 1000mL of deionized water for high-temperature sterilization. 5mg of CdCl was taken 2 The powder was added to 1L of the medium to adjust the final concentration to 5mg/L, and the mixture was dispensed into 250mL Erlenmeyer flasks at a volume of 100mL per flask. Adding 1mL of delta PS04-17 bacterial liquid into the mixtureCulturing in culture medium, collecting supernatant at 4 th and 8 th days, and adding CdCl containing 5mg/L 2 The liquid medium was used as a control group, and the cadmium content was measured by flame spectrophotometry.
CdCl of delta PS04-17 strain at 5mg/L 2 The growth in liquid medium is shown in FIG. 8. The results show that: compared with a control group, the delta PS04-17 strain can obviously reduce the cadmium content in the supernatant, and the cadmium ion content in the 8d supernatant is obviously reduced by 77 percent (p)<0.05)。
Example 6 inactivation of cadmium in Dry soil by the Δ PS04-17 Strain
The cadmium polluted soil sample collected from a certain place of Shaoguan is placed indoors for natural air drying, impurities such as plant residues, stones and the like are removed, and the cadmium polluted soil sample is ground and sieved by a 60-mesh nylon sieve after being uniformly mixed. 200g of soil is weighed and added into a beaker, and the bacterial liquid (OD) after delta PS04-17 fermentation is added 600 = 2.0), the diluted bacterial solution was added in 5 treatments of 0.1, 1.0, 10, 100, and 200mL, respectively, and sterile water alone was used as a control. Each beaker was filled with sterile water to 200mL and each treatment was repeated three times. Fully stirring the mixture evenly, standing the mixture, replenishing water every day and keeping the water level of each treatment consistent. And (4) respectively taking soil at 1 st week after the treatment, and measuring the content of the water-soluble cadmium by using an atomic absorption method.
As shown in FIG. 9, the concentrations of water-soluble cadmium in the soil were reduced to 0.05ppm, both after 0.1 mL and 1mL of the diluted bacterial suspension of Δ PS04-17 were added. The concentration of the water-soluble cadmium in the control group reaches 0.16ppm, the reduction range of the water-soluble cadmium reaches 68.75 percent (p is less than 0.05), and the effect is very obvious.
In conclusion, the invention provides a new application of the paenibacillus kribbensis space mutant delta PS04-17 in the aspect of repairing or passivating cadmium. The research of the invention shows that the delta PS04-17 strain has super cadmium-resistant capability, can grow well in a cadmium-containing culture medium, and has stronger cadmium-resistant capability than that of wild PS04; meanwhile, the delta PS04-17 strain can relieve the cadmium stress of the rice and promote the growth of the rice under the cadmium stress, and the delta PS04-17 strain can also obviously reduce the cadmium content and has a fixing effect on cadmium ions; and the concentration of soluble cadmium in the soil can be reduced, and the cadmium-removing agent is used for relieving the stress of cadmium plants or repairing the soluble cadmium in the soil. According to the invention, through research on mutant delta PS04-17 strains of Paenibacillus kribbensis, the tolerance of the delta PS04-17 strains to cadmium is remarkably improved, cadmium ions can be fixed and passivated very low, the strains can be used for repairing soil heavy metals, treating cadmium-polluted soil, eliminating stress on plants, promoting plant growth, and have important application significance for repairing and utilizing the cadmium-polluted soil.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The application of a Paenibacillus kriibbensis space mutation mutant strain delta PS04-17 strain or a bacterial liquid thereof in adsorption or passivation of cadmium ions.
2. Application of a Paenibacillus kribbensis space mutation mutant strain delta PS04-17 strain or a bacterial liquid thereof in preparation of cadmium ion passivator and/or a bacterial agent for reducing cadmium content.
3. Application of a Paenibacillus kribbensis space mutation mutant strain delta PS04-17 strain or a bacterial liquid thereof in relieving cadmium stress of plants and/or promoting plant growth under the cadmium stress.
4. Application of a Paenibacillus kribbensis space mutation mutant strain delta PS04-17 strain or a bacterial solution thereof in remediation of soil cadmium pollution.
5. Application of Paenibacillus kribbensis space mutation mutant strain delta PS04-17 strain or bacterial liquid thereof in preparation of soil cadmium pollution remediation agent.
6. The use according to any one of claims 1 to 5, wherein the Paenibacillus kribbensis space mutation mutant strain Δ PS04-17 strain is deposited at 17.3.2022 in Guangdong province collection center for microbial cultures, with the deposit number being GDMCC NO:62233 and the deposit address being Lou 5 of No. 59 of Mirabilite 100 of Guangdong province.
7. A method for fixing cadmium ions in solution is characterized in that a bacillus kribbensis space mutation mutant strain delta PS04-17 bacterial solution and a sample solution are adopted for co-culture treatment.
8. A method for relieving cadmium stress of plants or repairing cadmium pollution of soil is characterized in that a paenibacillus kribbensis space mutation mutant strain delta PS04-17 bacterial liquid is adopted to treat roots of the plants or the soil.
9. The method according to claim 7 or 8, wherein the bacterial liquid is a fermentation liquid.
10. The method of claim 9, wherein the fermentation broth OD = 1.0-2.0, the fermentation broth concentration is 1-10%, and the fermentation conditions are as follows: inoculating the delta PS04-17 bacterial liquid into a culture medium, fermenting for 3-4 days at 25-30 ℃ and 150-200 rpm.
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