CN115737888B - Composite protein slow-release hydrocolloid application - Google Patents

Composite protein slow-release hydrocolloid application Download PDF

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Publication number
CN115737888B
CN115737888B CN202211370216.8A CN202211370216A CN115737888B CN 115737888 B CN115737888 B CN 115737888B CN 202211370216 A CN202211370216 A CN 202211370216A CN 115737888 B CN115737888 B CN 115737888B
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composite protein
silk fibroin
hydrocolloid
release
mussel mucin
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CN115737888A (en
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周晓宁
赵铭
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Deshengkang Suzhou Biotechnology Co ltd
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Deshengkang Suzhou Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F13/00—Bandages or dressings; Absorbent pads
    • A61F13/02—Adhesive bandages or dressings
    • A61F13/0276—Apparatus or processes for manufacturing adhesive dressings or bandages
    • A61F13/0289—Apparatus or processes for manufacturing adhesive dressings or bandages manufacturing of adhesive dressings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/28—Polysaccharides or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/602—Type of release, e.g. controlled, sustained, slow
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
    • A61L2300/622—Microcapsules

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Abstract

The application discloses a composite protein slow-release hydrocolloid application which is characterized in that raw material components of the hydrocolloid application comprise a water-absorbing high polymer material, a hot-melt pressure-sensitive adhesive and microspheres wrapped by composite protein, wherein the mass ratio of the microspheres in the hydrocolloid application is 5% -20%; the composite protein comprises silk fibroin and mussel mucin, and the mass ratio of the silk fibroin to the mussel mucin is 1:1-5:1. The composite protein slow-release hydrocolloid dressing combines the unique interface coverage characteristic of the silk fibroin and the adhesive property of the mussel mucin, and the volatile functional components in the hydrocolloid dressing are encapsulated in the composite protein consisting of the silk fibroin and the mussel mucin, so that the release rate of the volatile components can be effectively reduced, and the controlled release at different stages can be realized.

Description

Composite protein slow-release hydrocolloid application
The application relates to a compound protein slow-release hydrocolloid dressing which is applied for 22 days of 2022 and 03 months, has the application number of 2022102873064 and is named as a preparation method thereof.
Technical Field
The application belongs to the technical field of medical application, and particularly relates to a compound protein slow-release hydrocolloid application.
Background
The hydrocolloid dressing is a novel dressing material based on the wet healing theory, and is prepared by blending a water-absorbing polymer material with a hot-melt pressure-sensitive adhesive, and the material combines the adhesive property of the hot-melt pressure-sensitive adhesive and the water absorption of the water-absorbing polymer material. The hot-melt pressure-sensitive adhesive provides adhesiveness, so that the application can be stuck on a wound surface, and the water-absorbing polymers dispersed in the adhesive can swell after absorbing water, so that a moist closed environment is provided for the wound, and the healing of the wound is promoted.
In the prior art, in order to provide more functionality and comfort for the hydrocolloid dressing, various components such as essence and essential oil are often required to be added into the hydrocolloid dressing, and the components are generally composed of tens or even hundreds of volatile substances such as aldehydes, ketones, alcohols and esters, and can be continuously released from the hydrocolloid dressing. However, the molding of the hydrocolloid is performed by a heating and melting process, and the ingredients such as essence, essential oil and the like are directly mixed with the hydrocolloid, so that the loss of volatile ingredients is caused in the molding process. In addition, these components can still volatilize continuously before application, further affecting the effect in actual use. Therefore, how to control the release of volatile components is a problem to be solved in hydrocolloid applicator products.
The silk fibroin is a natural protein extracted from silk, and structurally has a hydrophilic area and a hydrophobic area, so that the silk fibroin has amphiphilic property and has certain surface activity, namely, the silk fibroin self-assembles at a fluid interface and forms a stable viscoelastic surface or interface layer, and the silk fibroin has strong covering capacity. Mussels are shellfish marine organisms widely distributed on coastal and offshore, the foot silk glands can secrete foot silks, the mussels are fixed on the surfaces of various solid substrates through foot silk discs formed by the foot silks, and mussel mucin is the main component of the mussel foot silks, so that the mussel has strong adhesive property.
Disclosure of Invention
In view of the above, in order to overcome the defects of the prior art, the application aims to provide a composite protein slow-release hydrocolloid patch, which combines the unique interface coverage characteristic of silk fibroin and the adhesive property of mussel mucin so as to control the release of volatile components.
In order to achieve the above purpose, the present application adopts the following technical scheme:
the composite protein slow-release hydrocolloid dressing comprises raw material components of a water-absorbing polymer material, a hot-melt pressure-sensitive adhesive and microspheres wrapped by composite protein, wherein the mass ratio of the microspheres in the hydrocolloid dressing is 5% -20%; the composite protein comprises silk fibroin and mussel mucin, and the mass ratio of the silk fibroin to the mussel mucin is 1:1-5:1. The silk fibroin is deposited on the surface of the functional material through amphipathy to form a shell body, and mussel mucin is deposited on the surface of the silk fibroin through electrostatic adsorption to play a role in reinforcement. If the content of mussel mucin is too low, the mussel mucin cannot function, and if the content is too high, the microspheres can be adhered to each other.
According to some preferred embodiments of the application, the microsphere contains a functional material, and the mass ratio of the functional material to the silk fibroin is 5:1-15:1. The size and the loading capacity of the microspheres are determined, if the proportion of the functional materials is too low, the microspheres are too small, the loading capacity is low, and if the proportion of the functional materials is too high, the microspheres are too large and are easy to damage.
According to some preferred embodiments of the application, the functional material is a perfume and/or an essential oil.
According to some preferred embodiments of the present application, the mass ratio of the water-absorbing polymer material and the hot-melt pressure-sensitive adhesive in the hydrocolloid dressing is 5% to 40% and 40% to 90%, respectively. The proportion setting can give consideration to the strength and the water absorption performance of the hydrocolloid dressing, if the proportion of the water absorption polymer material is too low, the liquid seepage absorption capacity of the dressing is reduced, the wound cannot be kept moist, and if the proportion of the water absorption polymer material is too high, the cohesion of the hot melt pressure sensitive adhesive is reduced, and the dressing is easy to crack.
According to some preferred embodiments of the present application, the water-absorbing polymer material is a mixture of one or more of modified starch, acacia, gelatin, pectin, sodium polyacrylate, polyacrylamide, sodium carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl alcohol, and polyvinylpyrrolidone; the hot-melt pressure-sensitive adhesive is a mixture composed of one or more of natural rubber, polyurethane rubber, silicon rubber, polyisobutylene rubber, a styrene block copolymer thermoplastic elastomer, polyacrylate and polyvinyl ether.
The application also provides a preparation method of the composite protein slow-release hydrocolloid dressing, which comprises the following steps:
adding the functional material into the silk fibroin aqueous solution and uniformly mixing, then adding the mussel mucin aqueous solution and uniformly mixing, adding ethanol into the system, continuously stirring, and then centrifuging and cleaning to obtain the composite protein-coated microsphere; and (3) blending the microspheres wrapped by the composite protein with a water-absorbing polymer material and a hot-melt pressure-sensitive adhesive, and extruding and calendaring to obtain the composite protein slow-release hydrocolloid application.
According to some preferred embodiments of the application, the aqueous silk fibroin solution has a mass percentage concentration of 2% -15%. If the concentration of the aqueous solution of silk fibroin is too low, enough silk fibroin cannot be deposited on the surface of the functional material, and if the concentration is too high, the solution viscosity is too high, so that the microsphere cannot be prepared.
According to some preferred embodiments of the application, the ratio of the functional material to the aqueous silk fibroin solution (i.e. the mass ratio to the silk fibroin solution) is 1:10-1:50. Within this range the functional material forms a stable emulsion with the aqueous silk fibroin solution. The aforementioned "mass ratio of functional material to silk fibroin is 5:1-15:1" corresponds to the mass ratio of functional material to silk fibroin in the microsphere, and the corresponding here is the mass ratio added in the preparation process, because silk fibroin solution is not 100% available in the preparation, the aforementioned calculation is that silk fibroin deposited on the surface of the essential oil microsphere, and the rest remains in solution.
According to some preferred embodiments of the application, the concentration of the mussel mucin aqueous solution is 2% -15% by mass. If the concentration is too low, the solution cannot be compounded with enough silk fibroin, and if the concentration is too high, the solution viscosity is too high, so that the solution is not easy to mix.
According to some preferred embodiments of the application, the ethanol is added in an amount of 10% -30% of the total volume of the mixed solution formed by the functional material, the aqueous silk fibroin solution and the aqueous mussel mucin solution. The ethanol is used for converting the silk fibroin into a beta-sheet conformation, so that the silk fibroin is promoted to form a shell layer of the microsphere, and the microsphere is more stable in an aqueous solution. If the ethanol addition amount is too small, silk fibroin cannot form a shell layer, and if the ratio exceeds the above ratio, the ethanol addition amount is increased at the same time, and the appearance of the shell layer is not changed obviously.
In some embodiments of the present application, the method for preparing a composite protein slow-release hydrocolloid dressing specifically comprises the following steps:
1. preparing aqueous solution of silk fibroin, wherein the mass percentage concentration of the aqueous solution is 2% -15%.
2. Adding essential oil into silk fibroin aqueous solution, and stirring with a high-speed homogenizer to form white emulsion, wherein the ratio of essential oil to silk fibroin aqueous solution is 1:10-1:50.
3. Preparing mussel mucin aqueous solution with the mass percentage concentration of 2-15%. Adding mussel mucin aqueous solution into the emulsion, and stirring at room temperature for 24h.
4. Adding ethanol into the solution, wherein the addition amount is 10% -30% of the total amount of the solution, continuously stirring for 12h, and then centrifuging and cleaning to obtain the essential oil microsphere wrapped by the composite protein. The particle size of the microsphere is 10-100 μm. Wherein, the ethanol is used for converting the silk fibroin into beta-sheet conformation, promoting the silk fibroin to form a shell layer of the microsphere, and leading the microsphere to be more stable in aqueous solution.
5. And blending the essential oil microspheres with a hot-melt pressure-sensitive adhesive and a hydrocolloid, extruding by a double screw, and calendaring to obtain the composite protein slow-release hydrocolloid patch.
The principle of the application is as follows: the volatile components of the functional materials such as essential oil are insoluble in water and are oil phases, the functional materials are blended with water under the action of strong shearing, the oil phases can form tiny liquid drops to be dispersed in the water phases, and the silk fibroin has amphipathy and can be wrapped on the surfaces of the oil drops. In addition, the surface of the silk fibroin is negatively charged in a neutral aqueous solution, and after the positively charged mussel mucin is added, the mussel mucin can automatically deposit on the surface of the silk fibroin, and finally the silk fibroin-mussel mucin composite protein coated essential oil microsphere is formed. After the mussel mucin is deposited on the surface of the silk fibroin, the dopa groups thereof are self-crosslinked to form a net structure, so that the silk fibroin shell is reinforced, and the microsphere is more stable.
The microsphere is blended with hot-melt pressure-sensitive adhesive and water-absorbing polymer material such as hydrocolloid to obtain hydrocolloid dressing, and the escape rate of essential oil is obviously lower than that of hydrocolloid dressing obtained by directly blending essential oil with hot-melt pressure-sensitive adhesive and hydrocolloid when the dressing is not used. The hydrocolloid patch obtained by the application is applied to the skin surface, after the hydrocolloid and the compound protein absorb water, the compound protein shell layer swells, the intermolecular distance is increased, the permeability of the compound protein is increased, and the release of essential oil is accelerated, so that the controllable release of volatile components is realized.
Due to the implementation of the technical scheme, compared with the prior art, the application has the following advantages: the composite protein slow-release hydrocolloid dressing combines the unique interface coverage characteristic of the silk fibroin and the adhesive property of the mussel mucin, and the volatile functional components in the hydrocolloid dressing are encapsulated in the composite protein consisting of the silk fibroin and the mussel mucin, so that the release rate of the volatile components can be effectively reduced, and the controlled release at different stages can be realized.
Detailed Description
In order to better understand the technical solutions of the present application, the following description will clearly and completely describe the technical solutions of the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.
Example 1
The raw material components of the composite protein slow-release hydrocolloid dressing in the embodiment comprise a hydrocolloid, a hot-melt pressure-sensitive adhesive and microspheres wrapped by composite proteins. Wherein, the mass ratio of the microsphere in the hydrocolloid is 10%, the mass ratio of the water absorbing polymer in the hydrocolloid is 25%, and the mass ratio of the hot melt pressure sensitive adhesive in the hydrocolloid is 65%.
The composite protein comprises silk fibroin and mussel mucin, and the mass ratio of the silk fibroin to the mussel mucin is 3:1.
The composite protein-coated microsphere in the embodiment is an essential oil microsphere coated by composite protein, wherein the mass ratio of essential oil to silk fibroin is 9:1.
The preparation method of the composite protein slow-release hydrocolloid dressing in the embodiment specifically comprises the following steps:
1. preparing aqueous solution of silk fibroin, wherein the mass percentage concentration is 10%.
2. Adding essential oil into silk fibroin aqueous solution, and stirring with a high-speed homogenizer (stirring speed is 5000 RPM) to form white emulsion, wherein the ratio of essential oil to silk fibroin aqueous solution is 1:10.
3. Preparing mussel mucin aqueous solution with the mass percentage concentration of 10 percent. Adding mussel mucin aqueous solution into the emulsion, and stirring at room temperature for 24h.
4. Adding ethanol into the solution, wherein the addition amount is 10% of the total amount of the solution, continuously stirring for 12h, and then centrifuging and cleaning to obtain the essential oil microsphere wrapped by the composite protein.
5. And blending the essential oil microspheres with a hot-melt pressure-sensitive adhesive and a hydrocolloid, extruding by a double screw, and calendaring to obtain the composite protein slow-release hydrocolloid patch.
Example 2
The raw material components of the composite protein slow-release hydrocolloid dressing in the embodiment comprise a hydrocolloid, a hot-melt pressure-sensitive adhesive and microspheres wrapped by composite proteins. Wherein, the weight ratio of the microsphere in the hydrocolloid is 5%, the weight ratio of the water absorbing polymer in the hydrocolloid is 40%, and the weight ratio of the hot melt pressure sensitive adhesive in the hydrocolloid is 55%.
The composite protein comprises silk fibroin and mussel mucin, and the mass ratio of the silk fibroin to the mussel mucin is 5:1.
The composite protein-coated microsphere in the embodiment is an essential oil microsphere coated by composite protein, wherein the mass ratio of essential oil to silk fibroin is 9:1.
The preparation method of the composite protein slow-release hydrocolloid dressing in the embodiment specifically comprises the following steps:
1. preparing aqueous solution of silk fibroin, wherein the mass percentage concentration is 5%.
2. Adding essential oil into silk fibroin aqueous solution, and stirring with a high-speed homogenizer (stirring speed is 5000 RPM) to form white emulsion, wherein the ratio of essential oil to silk fibroin aqueous solution is 1:10.
3. Preparing mussel mucin aqueous solution with the mass percentage concentration of 2 percent. Adding mussel mucin aqueous solution into the emulsion, and stirring at room temperature for 24h.
4. Adding ethanol into the solution, wherein the addition amount is 10% of the total amount of the solution, continuously stirring for 12h, and then centrifuging and cleaning to obtain the essential oil microsphere wrapped by the composite protein.
5. And blending the essential oil microspheres with a hot-melt pressure-sensitive adhesive and a hydrocolloid, extruding by a double screw, and calendaring to obtain the composite protein slow-release hydrocolloid patch.
Example 3
The raw material components of the composite protein slow-release hydrocolloid dressing in the embodiment comprise a hydrocolloid, a hot-melt pressure-sensitive adhesive and microspheres wrapped by composite proteins. Wherein, the mass ratio of the microsphere in the hydrocolloid is 20%, the mass ratio of the water absorbing polymer in the hydrocolloid is 20%, and the mass ratio of the hot melt pressure sensitive adhesive in the hydrocolloid is 60%.
The composite protein comprises silk fibroin and mussel mucin, and the mass ratio of the silk fibroin to the mussel mucin is 1:1.
The composite protein-coated microsphere in the embodiment is an essential oil microsphere coated by composite protein, wherein the mass ratio of essential oil to silk fibroin is 6:1.
The preparation method of the composite protein slow-release hydrocolloid dressing in the embodiment specifically comprises the following steps:
1. preparing aqueous solution of silk fibroin, wherein the mass percentage concentration is 10%.
2. Adding essential oil into silk fibroin aqueous solution, and stirring with a high-speed homogenizer (stirring speed is 5000 RPM) to form white emulsion, wherein the ratio of essential oil to silk fibroin aqueous solution is 1:10.
3. Preparing mussel mucin aqueous solution with the mass percentage concentration of 5 percent. Adding mussel mucin aqueous solution into the emulsion, and stirring at room temperature for 24h.
4. Adding ethanol into the solution, wherein the addition amount is 10% of the total amount of the solution, continuously stirring for 12h, and then centrifuging and cleaning to obtain the essential oil microsphere wrapped by the composite protein.
5. And blending the essential oil microspheres with a hot-melt pressure-sensitive adhesive and a hydrocolloid, extruding by a double screw, and calendaring to obtain the composite protein slow-release hydrocolloid patch.
Comparative example 1
The difference between this comparative example and example 1 is that: the mass ratio between silk fibroin and mussel mucin in this comparative example was 1:2. Other formulation ingredients and preparation steps were substantially the same as in example 1. In the comparative example, microspheres are mutually adhered, agglomerated and unevenly dispersed in the preparation process.
Comparative example 2
The difference between this comparative example and example 1 is that: the mass ratio between the essential oil and the silk fibroin in this comparative example was 2:1. Other formulation ingredients and preparation steps were substantially the same as in example 1.
Comparative example 3
The difference between this comparative example and example 1 is that: the concentration of the aqueous silk fibroin solution in this comparative example was 17%. Other formulation ingredients and preparation steps were substantially the same as in example 1. In the comparative example, the deposition of silk fibroin and mussel mucin on the surface of essential oil liquid drops is blocked due to overlarge solution viscosity in the preparation process, so that microspheres cannot be formed.
Comparative example 4
The difference between this comparative example and example 1 is that: the amount of ethanol added in this comparative example was 5%. Other formulation ingredients and preparation steps were substantially the same as in example 1.
Comparative example 5
The difference between this comparative example and example 1 is that: the microspheres in the comparative example do not contain mussel mucin, and the mussel mucin aqueous solution is not required to be added in the preparation process. Other formulation ingredients and preparation steps were substantially the same as in example 1.
Comparative example 6
In this comparative example, the essential oil microspheres in example 1 were replaced with the same quality of essential oil, and added to a hot-melt pressure-sensitive adhesive and hydrocolloid to be blended and extruded and prolonged to obtain a hydrocolloid application.
Testing and results
The hydrocolloid patches prepared in the examples and the comparative examples with the same quality are respectively dissolved in ethyl acetate, and the initial content of the essential oil is measured by ultraviolet; and in the unused state, placing the patch in normal saline at 25 ℃ under room temperature for 6 months, and periodically detecting the content of residual essential oil. The essential oil content is calculated by the following method: the essential oil is dissolved in ethyl acetate to prepare standard solutions with different concentrations, a spectrophotometry is used for measuring absorbance to obtain a standard curve, then a sample to be measured is dissolved in ethyl acetate to measure absorbance, and the concentration is calculated by using the standard curve. The results are shown in Table 1.
Table 1 test results
As can be seen from the test results of table 1, the loss of the essential oil is lower in the process of blending thermoforming with the hydrocolloid and the hot melt pressure sensitive adhesive by using the composite protein-coated essential oil compared with the essential oil not subjected to protein coating; the release amount of the essential oil coated by the composite protein is obviously lower than that of the hydrocolloid patch obtained by direct blending; and the essential oil is applied to the skin surface, and after the hydrocolloid absorbs the seepage and water vapor, the release rate of the essential oil is accelerated, so that the controllable release can be realized.
Aiming at the problem that the volatilization rate of volatile components such as essence and essential oil is too high after the volatile components are directly added into the conventional hydrocolloid dressing, the application designs a slow-release structure by utilizing the composite protein of silk fibroin and mussel mucin, can encapsulate the volatile components in the composite protein, can effectively reduce the release rate of the volatile components, and can realize the controlled release at different stages.
The above embodiments are only for illustrating the technical concept and features of the present application, and are intended to enable those skilled in the art to understand the present application and to implement the same, but are not intended to limit the scope of the present application, and all equivalent changes or modifications made according to the spirit of the present application should be included in the scope of the present application.

Claims (6)

1. The composite protein slow-release hydrocolloid dressing is characterized by being prepared by the following preparation method: blending the microspheres wrapped by the composite protein with a water-absorbing polymer material and a hot-melt pressure-sensitive adhesive, and extruding, calendaring and forming to obtain the composite protein slow-release hydrocolloid application; the composite protein comprises silk fibroin and mussel mucin, wherein the mass ratio of the silk fibroin to the mussel mucin in the composite protein is 1:1-5:1; the microsphere contains functional materials; the mass ratio of the functional material to the silk fibroin is 5:1-15:1; the functional material is essence and/or essential oil.
2. The composite protein sustained-release hydrocolloid dressing of claim 1, wherein the mass ratio of the microspheres wrapped by the composite protein in the hydrocolloid dressing is 5% -20%; and/or the mass ratio of the water-absorbing polymer material in the hydrocolloid dressing is 5% -40%; and/or the mass ratio of the hot-melt pressure-sensitive adhesive in the hydrocolloid dressing is 40% -90%.
3. The composite protein sustained-release hydrocolloid dressing of claim 1, wherein the particle size of the composite protein-coated microspheres is 10-100 μm.
4. The composite protein sustained-release hydrocolloid dressing of claim 1, wherein the composite protein-coated microspheres are prepared by: adding the functional material into the silk fibroin aqueous solution, uniformly mixing, then adding the mussel mucin aqueous solution, uniformly mixing, adding ethanol into the system, continuously stirring, and then centrifuging and cleaning to obtain the composite protein coated microsphere.
5. The composite protein slow-release hydrocolloid application according to claim 4, wherein the mass percentage concentration of the aqueous silk fibroin solution is 2% -15%; and/or, the concentration of the mussel mucin aqueous solution in mass percent is 2% -15%.
6. The composite protein sustained-release hydrocolloid dressing of claim 4, wherein the amount of ethanol added is 10% -30% of the total volume of the mixed solution formed by the functional material, the aqueous silk fibroin solution and the aqueous mussel mucin solution.
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