CN115721869A - Optogenetic system for peripheral nerve stimulation - Google Patents
Optogenetic system for peripheral nerve stimulation Download PDFInfo
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- CN115721869A CN115721869A CN202211485233.6A CN202211485233A CN115721869A CN 115721869 A CN115721869 A CN 115721869A CN 202211485233 A CN202211485233 A CN 202211485233A CN 115721869 A CN115721869 A CN 115721869A
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Abstract
The present application discloses a optogenetic system for peripheral nerve stimulation, the system comprising: the device comprises a light stimulation module, a terminal control module and an upper computer; the light stimulation module comprises a mu LED and leads connected with the anode and the cathode of the mu LED, and is implanted into a site to be stimulated and used for regulating and controlling the neuron activity of the site; the terminal control module comprises a terminal circuit board and a battery, the terminal circuit board is connected with the lead, the battery is detachably connected with the terminal circuit board, and the terminal control module is fixed on the experimental body and used for controlling the mu LED; and the upper computer establishes wireless communication with the terminal control module and is used for inputting optical stimulation parameters to act on the experimental body and receiving the working state information of the mu LED. That is, the optogenetic system of this application convenient to use does not receive the experiment body action restriction, satisfies the experiment demand, has improved optogenetic system operability.
Description
Technical Field
The application relates to the field of medical equipment, in particular to a optogenetic system for peripheral nerve stimulation.
Background
Optogenetic technology is a neural loop regulation technology with high spatial and temporal resolution. It expresses channel proteins capable of responding to light in specific cells by means of genetic means, and achieves the aim of activating or inhibiting the activity of neurons by light. Since the invention of Karl Deisseroth laboratory at Stanford university in 2005, the optogenetic technology plays an important role in neural loop analysis and brain mapping.
In the central nervous system, relatively fixed tissues and skull provide convenience for the implantation of quartz fiber, the application of optogenetic technology is well established, and in peripheral nerves, the implementation of optogenetic stimulation requires flexible technology to cope with the activity of tissues.
The flexible technology has two development directions of flexible optical fiber and mini LED (called mu LED below), wherein the scheme of the flexible optical fiber can output stronger monochromatic light and generate less heat, but needs to be externally connected with a laser transmitter, and a connecting wire can interfere the activity of animals; the mu LED solution can achieve wireless power supply and signal transmission, but needs to balance between various constraints of heat generation and light intensity, size and quality, function and endurance.
Therefore, the existing optogenetic system for peripheral nerve photostimulation experiments is difficult to meet the requirements of neuroscience experiments, and has low operability.
Disclosure of Invention
In view of this, the embodiment of the present application provides an optogenetic system for peripheral nerve stimulation, which aims to solve the technical problems that the existing optogenetic system for peripheral nerve stimulation is low in operability and difficult to meet experimental requirements.
An embodiment of the present application provides a optogenetic system for peripheral nerve stimulation, the system comprising: the system comprises a light stimulation module, a terminal control module and an upper computer;
the light stimulation module comprises a mu LED and leads connected with the anode and the cathode of the mu LED, and is implanted into a site to be stimulated and used for regulating and controlling the neuron activity of the site;
the terminal control module comprises a terminal circuit board and a battery, the terminal circuit board is connected with the lead, the battery is detachably connected with the terminal circuit board, and the terminal control module is fixed on the experimental body and used for controlling the mu LED;
and the upper computer establishes wireless communication with the terminal control module and is used for inputting optical stimulation parameters to act on the experimental body and receiving the working state information of the mu LED.
In one possible embodiment of the present application, the process of preparing the lead wire includes:
leading out the positive electrode and the negative electrode of the mu LED by using a gold wire, and winding the gold wire to form a spiral gold wire;
encapsulating the mu LED and the positive and negative pins thereof by using prepared glue;
and encapsulating the spiral gold wire by using polydimethylsiloxane to form the lead.
In a possible embodiment of the present application, the leading out the positive electrode and the negative electrode of the μ LED by using a gold wire, and winding the gold wire to form a spiral gold wire, includes:
a metal wire which can be dissolved in hydrochloric acid and the gold wire are wound into a spiral shape together;
and (3) putting the wound metal wire and gold wire into dilute hydrochloric acid for dissolving, and dissolving the metal wire to obtain the spiral gold wire.
In one possible embodiment of the present application, the encapsulating the spiral gold wire with polydimethylsiloxane, in the forming of the lead, includes:
coated on the spiral gold wire using a first polydimethylsiloxane;
coating a second polydimethylsiloxane on the coated spiral gold wire to form the lead wire;
wherein the first polydimethylsiloxane has a higher viscosity than the second polydimethylsiloxane.
In a possible embodiment of the present application, after the step of leading out the positive and negative electrodes of the μ LED by using a gold wire, and before the step of winding the gold wire to form a spiral gold wire, the gold wire is packaged by using parylene, and then the spiral gold wire after winding is coated with second dimethicone to form the lead wire.
In a possible implementation manner of the application, a low-power-consumption Bluetooth chip, a passive crystal oscillator and a ceramic patch antenna are arranged on the terminal circuit board, and the ceramic patch antenna is communicated with the upper computer.
In one possible embodiment of the present application, the bluetooth low energy chip includes a plurality of output pins, and the output pins are connected to the μ LED for individually controlling parameters and status feedback of the μ LED.
In a possible embodiment of the present application, the battery is detachably connected to the terminal circuit board in a pin header-pin header type, the pin header is connected to the terminal circuit board, and the pin header is connected to the battery.
In a possible embodiment of the present application, when the battery and the terminal circuit board are installed independently, the battery and the terminal circuit board are connected with a flexible wire detachably, and two ends of the flexible wire are connected with the battery and the terminal circuit board respectively.
In a possible embodiment of the present application, the optical stimulation module is implanted into a site to be stimulated, and is configured to select a shape corresponding to a fixing material according to a shape of the site during a process of regulating a neuron activity of the site, where the fixing material includes a self-curling material and a tissue glue.
The present application provides a optogenetic system for peripheral nerve stimulation, the system comprising: the device comprises a light stimulation module, a terminal control module and an upper computer; the light stimulation module comprises a mu LED and leads connected with the anode and the cathode of the mu LED, and is implanted into a site to be stimulated and used for regulating and controlling the neuron activity of the site; the terminal control module comprises a terminal circuit board and a battery, the terminal circuit board is connected with the lead, the battery is detachably connected with the terminal circuit board, and the terminal control module is fixed on the experimental body and used for controlling the mu LED; the upper computer is in wireless communication with the terminal control module and is used for inputting optical stimulation parameters to act on the experimental body and receiving the working state information of the mu LED. That is, in this application, in the optogenetic system for peripheral nerve stimulation that comprises light stimulation module, terminal control module and host computer, through terminal control module control mu LED and feed back its operating condition, and terminal control module and host computer wireless communication, can realize the optogenetic experimental control of the experimental part through the host computer, acquire relevant information in real time. Meanwhile, the terminal control module can work through battery plug-in connection, is convenient to use and not limited by the behavior of an experimental body, meets the experimental requirement and improves the operability of the optogenetic system.
Drawings
FIG. 1 is a schematic diagram of the architecture of the optogenetic system for peripheral nerve stimulation of the present application;
FIG. 2 is a schematic diagram of a terminal control module of the optogenetic system for peripheral nerve stimulation according to the present application;
fig. 3 is a schematic illustration of the encapsulation of the μ LED and its leads of the present optogenetic system for peripheral nerve stimulation.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
In an embodiment of the present optogenetic system for peripheral nerve stimulation, referring to fig. 1, the system includes: the device comprises a light stimulation module 10, a terminal control module 20 and an upper computer 30.
As an example, the optical stimulation module comprises a μ LED11 and leads 12 connected to the positive and negative electrodes of the μ LED, and the optical stimulation module is implanted at a site to be stimulated for regulating the neuron activity at the site.
Wherein, the μ LED refers to a mini LED. The optogenetic experiment is especially important for the optogenetic experiment by implanting a miniature mu LED to perform photostimulation on a locus so as to regulate and control the neuron activity of the locus.
The site of the desired stimulus refers to a stimulus point on the test body for peripheral light stimulation in the test environment. It is noted that the subject may be a free-moving animal.
As an example, the terminal control module 20 includes a terminal circuit board 21 and a battery 22, the terminal circuit board 21 is connected to the lead 12, the battery 22 is detachably connected to the terminal circuit board 21, and the terminal control module 20 is fixed on the experimental body 100 and is used for controlling the μ LED.
The terminal control module is used for controlling the stimulation parameters of each mu LED to the site and receiving the working state of each mu LED of the site.
As an example, the operation states of the μ LED include light-up (including different brightness in light-up), and no light-up. Whether the mu LED is in a working state or not can be judged according to the lighting state of the mu LED, and whether the experiment of the experimental body is in a normal running state or not is further determined.
The terminal control module 20 comprises a terminal circuit board 21 and a battery 22, and the terminal circuit board 21 is connected with the lead 12 of the μ LED, so that the control of the μ LED by the terminal control module 20 and the recording of the working state of the μ LED are realized.
As an example, the terminal circuit may be a PCB board or a Flexible Printed Circuit (FPC), and the FPC is used to reduce the volume and mass of the circuit board and improve biocompatibility.
In the optogenetic system, power supply needs to be arranged to supply power to the mu LED so as to control the experimental body to be stimulated by light. In the existing optogenetic equipment, a micro LED light source and an electromagnetic induction coil power supply system are generally used, and a power supply coil needs to be placed outside a behavioristics experimental device, so that the experimental device is limited by an electromagnetic induction coil at a transmitting end, the size of the experimental device cannot exceed 30 × 30cm, and common experimental devices such as an overhead frame and an open field all exceed the size. Therefore, the rechargeable battery is detachably connected with the terminal circuit board, so that when the optogenetics experiment is carried out on the experimental body, the battery is connected with the terminal circuit board to supply power for the terminal circuit board, and when the optogenetics experiment is not carried out, the battery is separated from the terminal circuit board to charge the battery. Further, the mass and the volume of the ethology experimental device are reduced, so that the size and the animal posture of the ethology experimental device are not limited.
As an example, the upper computer establishes wireless communication with the terminal control module, and is configured to input optical stimulation parameters to act on the experimental body and receive operating state information of the μ LED.
As an example, the upper Computer may be a PC (Personal Computer), and may also be a smart terminal such as a mobile phone, so that the user inputs the optical stimulation parameters thereon and receives feedback such as the operating state of the μ LED. Wireless communication is established between the upper computer and the terminal control module, the terminal control module and the mu LED parameters can be controlled through the upper computer, feedback signals are received, the limitation of the size of a behavioural device is avoided, and the operability is high.
The optical stimulation parameters include frequency, pulse width, chip current (current supplied to the μ LED), and other parameters.
In this embodiment, the optogenetic system for peripheral nerve stimulation includes an optical stimulation module, a terminal control module, and an upper computer; the light stimulation module comprises a mu LED and leads connected with the anode and the cathode of the mu LED, and is implanted into a site to be stimulated and used for regulating and controlling the neuron activity of the site; the terminal control module comprises a circuit board and a battery, the circuit board is connected with the lead, the battery is detachably connected with the circuit board, and the terminal control module is fixed on the experimental body and used for controlling the mu LED; and the upper computer establishes wireless communication with the terminal control module and is used for inputting optical stimulation parameters to act on the experimental body and receiving the working state information of the mu LED. That is, in the optogenetic system for peripheral nerve stimulation composed of the optical stimulation module, the terminal control module and the upper computer, the mu LED is controlled by the terminal control module and the working state of the mu LED is fed back, the terminal control module is in wireless communication with the upper computer, optogenetic experiment control of an experimental body can be realized by the upper computer, and relevant information can be acquired in real time. Meanwhile, the terminal control module can work through battery plug-in connection, is convenient to use and not limited by the behavior of an experimental body, meets the experimental requirement and improves the operability of the optogenetic system.
As an example, the wireless communication established between the upper computer and the terminal control module may be bidirectional communication based on a bluetooth low energy protocol, the bluetooth low energy protocol is disposed in a bluetooth low energy chip, and the bluetooth low energy chip is disposed on the terminal circuit board. It can be understood that the communication distance between the upper computer and the terminal control module can reach ten meters based on the Bluetooth protocol and the transmitting power of the Bluetooth.
As an example, a low-power-consumption Bluetooth chip, a passive crystal oscillator and a ceramic patch antenna are arranged on the terminal circuit board, and the terminal circuit board is communicated with the upper computer through the ceramic patch antenna.
When the terminal control module communicates with the upper computer through the low-power-consumption Bluetooth chip, the terminal circuit board is further provided with a passive crystal oscillator, the passive crystal oscillator provides a clock of the low-power-consumption Bluetooth chip, and the ceramic patch antenna is located in the upper computer for communication.
As an example, the bluetooth low energy chip may be a CH582F chip, and the terminal circuit board PCB carries a circuit for μ LED parameter control, operation state detection, and bluetooth protocol communication with the CH582F chip as a core.
As an example, the bluetooth low energy chip comprises a plurality of output pins connected to the μ LED for individually controlling parameters and status feedback of the μ LED.
As an example, CH582F has 4 26-bit Timers (TMR), 6-way 8-bit Pulse Width Modulation (PWM), and 8-way 12-bit analog-to-digital converters (ADC), and CH582F has 20 General Purpose Input Output (GPIO) pins in total, so that the terminal board on each animal's head can support independent control and operational state feedback for four-way μ LEDs. One mu LED uses one GPIO pin, the other pin shares a ground pin (GND) with the other mu LED.
It should be noted that the idle GPIO pin of the CH582F chip, including the ADC, may be used for other stimulation outputs or signal acquisition.
If a plurality of sites need to be detected, the number of paths of output signals can be increased, namely GPIO pins are added, and more signal acquisition functions can be expanded.
In this embodiment, the bluetooth low energy chip has a unique ID, each GPIO pin of the bluetooth low energy chip has a unique ID, and the signal of each μ LED can be determined through the connection relationship between the μ LED and the GPIO pin of the bluetooth low energy chip. Therefore, each mu LED of each experimental body can be independently controlled in real time through the upper computer, and signals fed back by the mu LEDs are received and stored, so that the optogenetic system and other systems such as electrophysiological recording can be synchronized and linked.
It can be understood that each experimental body is implanted with a terminal circuit board for regulating and controlling the neuron activity of the optical stimulation site on the current experimental body, and the bluetooth low energy chip on each terminal circuit board has a unique ID. Therefore, the system can communicate with terminals with unique chip IDs on a plurality of animals by a low-power consumption Bluetooth (BLE) protocol through one upper computer, and has three functions of clock synchronization, parameter control input, feedback signal output and storage.
Where the clock synchronization is used to interface with other stimulation and recording systems, such as electrophysiological recording, behavioral videos, and the like.
The control parameters can adjust the stimulation pattern of each μ LED on each animal, such as whether it is on, current level, pulse or duration, pulse width and frequency.
The feedback signal, such as whether the mu LED is short or broken, can be displayed and stored on the upper computer in real time.
As an example, the terminal circuit board of the terminal control module is implanted at the skull of the head of the experimental body, or at a position such as the back and the like which can support the terminal circuit board and does not influence the action of the experimental body. When the terminal circuit board is implanted at the skull of the head of the experimental body, the circuit board is fixed on the skull at the top of the head of the experimental body through a cranial nail and preset glue (such as AB glue).
In this embodiment, a one-to-many real-time communication mechanism is performed between the upper computer and the terminal through the chip unique ID and the bluetooth low energy protocol, so that a plurality of experimental devices, a plurality of animals in each device, and a plurality of sites of each animal can be independently controlled, and the working state of the μ LED can be fed back to the host.
As an example, the battery is detachably connected to the terminal circuit board in a pin header-pin header type, the pin header is connected to the terminal circuit board, and the pin header is connected to the battery.
Referring to fig. 2, the terminal control module includes a terminal circuit board 21 and a battery 22, a pin header 211 is disposed on the terminal circuit board 21, the battery 22 is connected with a pin header 222 through a solder joint 221, the battery 22 and the pin header 222 are welded and fixed and packaged through an adhesive tape 23, and the pin header 211 is inserted into the pin header 222.
As an example, the area of the terminal circuit board can be below 7x7 mm, the terminal circuit board takes a CH582F chip as a core, a low dropout regulator (LDO) on the terminal circuit board reduces the voltage of about 3.7V provided by a battery to 3.3V required by the chip, a 32MHz passive crystal oscillator provides a chip clock, and a ceramic patch antenna is used for communicating with an upper computer.
The drive current for the muLED is provided by General Purpose Input Output (GPIO) pins corresponding to the 4 26 bit Timer (TMR) of the CH582F chip. According to the depth or distance of light rays emitted by the mu LED needing to penetrate in an experimental body, the circuit can select 5mA or 20mA current to supply power for the mu LED, and the mode can select pulse or continuous output. The pulse width and the period of the pulse mode can be freely adjusted between 1 mu s and 1s, and the accuracy of the crystal oscillator with +/-10 ppm is ensured. Mu LED operating state detection is provided by 4 of 8 ADC channels of the CH582F chip.
Due to the push-pull output characteristic of the CH582F chip, the experiment shows that the voltage at two ends of the mu LED is the highest (3.3V) when the mu LED is in an open circuit state and the voltage at two ends of the mu LED is the lowest when the mu LED is in a short circuit state, and the voltage at two ends of the mu LED is in the value between the highest voltage and the lowest voltage when the mu LED normally works, so that the difference is obvious. Therefore, the voltage applied to the two ends of the model mu LED used for measurement can determine the judgment threshold of the short circuit and the open circuit of the mu LED when the model mu LED works normally. For example, when the voltage across the μ LED is at the highest voltage, it indicates that the μ LED is open; if the voltage across the muLED is at the lowest voltage, it indicates that the muLED is shorted.
As an example, the battery is a polymer lithium battery commonly used for a Bluetooth headset, and has the size of 9x 4mm, the weight of 0.6g and the capacity of 40mAh, and can be used for more than two hours of experiments. Smaller cells can also be customized to further reduce mass and volume if desired. The battery and the terminal circuit board are connected in a pin-row female mode, the battery is inserted when the experiment starts, and the battery is recycled and charged after the experiment ends.
As an example, when the battery and the terminal circuit board are installed separately, the battery and the terminal circuit board are detachably connected by a flexible wire, and two ends of the flexible wire are respectively connected to the battery and the terminal circuit board.
The battery can be carried by the backpack, and the positive and negative electrodes of the battery are connected with the pin header/the female header by flexible leads. That is, the battery is connected with the terminal circuit board through a flexible lead.
In this embodiment, the control circuit uses a CH582F chip as a core, and a pluggable lithium polymer battery as a power supply module, and can independently control the multi-channel μ LEDs and feed back the operating states thereof. That is, can realize control through the host computer, terminal circuit board inserts the battery and can work convenient to use. Special emitting equipment (such as an electromagnetic induction coil, LED power supply or control equipment and the like) is not needed, high-precision technology and expensive elements are not needed in the manufacturing process, and the price is low.
As an example, the process of preparing the lead wire includes:
s10, leading out the anode and the cathode of the mu LED by using a gold wire, and winding the gold wire to form a spiral gold wire;
step S20, packaging the mu LED and the positive and negative pins thereof by using prepared glue;
and step S30, packaging the spiral gold wire by using polydimethylsiloxane to form the lead.
As an example, referring to fig. 3, fig. 3 is a schematic diagram of a μ LED and its lead. The nerve stimulation device comprises a target nerve or tissue 200 comprising a site to be stimulated, a self-curling material 201, a mu LED11, a spiral gold wire 202, PDMS203, a terminal circuit board 21, a bonding pad 204, soldering tin or silver paint 205 and ultraviolet curing glue 206.
And packaging the mu LED. Specifically, the positive and negative electrodes of the mu LED are LED out through a welding process, the other end of the gold wire is connected with the terminal circuit board, and the length of the connected lead is determined according to the requirements of different implantation sites. And encapsulating the mu LED and the positive and negative pins of the mu LED by preset glue.
As an example, since the glue is preset to act on a place near the μ LED, it is necessary to select a glue having good light transmittance and easy curing, such as an ultraviolet light curing glue. And packaging the mu LED and the pin thereof by using ultraviolet curing glue.
In order to avoid the interference of the lead formed by the gold wire with the activity of the experimental body and solve the problem that the probe connected with the light-emitting part in the existing wireless optogenetic system is hard, so that the system can only be applied to the central nervous system and can not be implanted into the periphery. Therefore, the gold wire is bent and packaged, so that the lead of the mu LED is flexible after being wound and packaged, the stretching after the peripheral implantation can be met, and meanwhile, the tissue damage is reduced.
Specifically, the gold wire is wound to form a spiral shape, so that a spiral gold wire is obtained, and the spiral gold wire is packaged by using polydimethylsiloxane to form a lead.
As an example, the soldering process may be a gold wire ball bonding process, and the step of leading the positive and negative electrodes of the μ LED out through the soldering process is to lead the positive and negative electrodes of the μ LED out through a gold wire with a diameter of about 20 μm by means of gold wire ball bonding.
As an example, the process of using a gold wire to lead out the positive electrode and the negative electrode of the μ LED and winding the gold wire to form a spiral gold wire includes:
s11, winding a metal wire which can be dissolved in hydrochloric acid and the gold wire into a spiral shape;
and S12, dissolving the wound metal wire and the gold wire in dilute hydrochloric acid to dissolve the metal wire, so as to obtain the spiral gold wire.
As an example, since the gold wire is a soft material and is not easily formed during the winding process, the metal wire can be used to assist the forming. Specifically, a metal wire and a gold wire of two pins of the mu LED are respectively wound into a spiral shape by a forming device such as a magnetic stirrer or an electric motor. And (3) putting the coiled metal wire and the gold wire into dilute hydrochloric acid for dissolving until only the coiled gold wire is left.
Wherein the wire is soluble in hydrochloric acid and has a diameter of about 50 μm. Such as 304 steel wire.
As an example, 3D spiral gold wires can be replaced with 2D serpentines for mass production using spin coating, photolithography, and the like. The 2D serpentine shaped gold wire can be made by a printing process.
As an example, the process of encapsulating the spiral gold wire by using polydimethylsiloxane to form the lead includes:
step S31, coating the metal wire on the spiral gold wire by using first polydimethylsiloxane;
step S32, coating second polydimethylsiloxane on the coated spiral gold wire to form the lead wire;
wherein the viscosity of the first polydimethylsiloxane is greater than that of the second polydimethylsiloxane.
As an example, the spiral gold wire is encapsulated by polydimethylsiloxane, and during the process of forming the lead, the spiral gold wire is encapsulated by double-layer Polydimethylsiloxane (PDMS), wherein the PDMS comprises first polydimethylsiloxane and second polydimethylsiloxane.
The first polydimethylsiloxane of the inner layer in the two PDMS materials is thicker when uncured, and can be conveniently coated on a gold thread, and the second polydimethylsiloxane of the outer layer has better elasticity, and can seal the whole spiral into a stretchable thin cylinder. It should be noted that, the end of the spiral gold wire is left unsealed by about 0.5cm, so as to facilitate the soldering after being wound on the terminal circuit board pin.
As an example, the first polydimethylsiloxane has a greater viscosity than the second polydimethylsiloxane. For example, the first polydimethylsiloxane of the inner layer uses DOWSIL TM SE 1700 type polydimethylsiloxane, the outer (conventional) second polydimethylsiloxane, SYLGARD 184 type polydimethylsiloxane, was used.
As an example, after the step of leading out the positive and negative electrodes of the μ LED using a gold wire, and before the step of winding the gold wire to form a spiral gold wire, the gold wire is encapsulated using parylene, and then the wound spiral gold wire is coated with second polydimethylsiloxane, so that the coating process of the spiral gold wire using the first polydimethylsiloxane is omitted, thereby forming a lead wire.
In this embodiment, the lead of the μ LED is wound into a spiral shape and encapsulated by PDMS, which has good elasticity and biocompatibility. When the mu LED is implanted on the experimental body, the luminous surface of the mu LED is fixed on the stimulation site through the self-curling material or the biocompatible glue.
As an example, the optical stimulation module is implanted into a site needing stimulation, and is used for selecting a shape corresponding to a fixing material according to the shape of the site in the process of regulating the neuron activity of the site, wherein the fixing material comprises a self-curling material and a tissue glue.
The μ LED in the photostimulation module and its connected leads pass through the tissue space and subcutaneously. Depending on the condition of the stimulation site, a self-curling material or a biocompatible glue (i.e. tissue glue) is chosen to fix the light emitting surface of the μ LED on the stimulation site. For example, if the nerve or tissue targeted by the stimulation site is on the surface of the bone of the subject and cannot be entangled, tissue glue is selected for adhesion. If the target nerve or tissue at the stimulation site is capable of being intertwined, a self-curling material is selected to mount the μ LED on the target nerve or tissue.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one of 8230, and" comprising 8230does not exclude the presence of additional like elements in a process, method, article, or apparatus comprising the element.
The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments.
Through the above description of the embodiments, those skilled in the art will clearly understand that the method of the above embodiments may be implemented by a software plus hardware platform, or may be implemented by hardware, but in many cases, the former is a better embodiment. Based on such understanding, the technical solutions of the present invention may be embodied in the form of a software product, which is stored in a storage medium (such as ROM/RAM, magnetic disk, optical disk) and includes instructions for enabling a terminal device (such as a mobile phone, a computer, a server, an air conditioner, or a network device) to execute the method according to the embodiments of the present invention.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A optogenetic system for peripheral nerve stimulation, the system comprising: the device comprises a light stimulation module, a terminal control module and an upper computer;
the light stimulation module comprises a mu LED and leads connected with the anode and the cathode of the mu LED, and is implanted into a site to be stimulated and used for regulating and controlling the neuron activity of the site;
the terminal control module comprises a terminal circuit board and a battery, the terminal circuit board is connected with the lead, the battery is detachably connected with the terminal circuit board, and the terminal control module is fixed on the experimental body and used for controlling the mu LED;
the upper computer is in wireless communication with the terminal control module and is used for inputting optical stimulation parameters to act on the experimental body and receiving the working state information of the mu LED.
2. The optogenetic system for peripheral nerve stimulation of claim 1, wherein the lead is prepared by a process comprising:
leading out the positive electrode and the negative electrode of the mu LED by using a gold wire, and winding the gold wire to form a spiral gold wire;
encapsulating the mu LED and the positive and negative pins thereof by using prepared glue;
and encapsulating the spiral gold wire by using polydimethylsiloxane to form the lead.
3. The optogenetic system for peripheral nerve stimulation according to claim 2, wherein the process of leading out the positive and negative electrodes of the μ LED by using a gold wire and winding the gold wire to form a spiral gold wire comprises:
a metal wire which can be dissolved in hydrochloric acid and the gold wire are wound into a spiral shape together;
and (3) putting the wound metal wire and the gold wire into dilute hydrochloric acid for dissolving, and dissolving the metal wire to obtain the spiral gold wire.
4. The optogenetic system of claim 2, wherein encapsulating the helical gold wire with polydimethylsiloxane, in forming the lead, comprises:
coated on the spiral gold wire using a first polydimethylsiloxane;
coating a second polydimethylsiloxane on the coated spiral gold wire to form the lead wire;
wherein the viscosity of the first polydimethylsiloxane is greater than that of the second polydimethylsiloxane.
5. The optogenetic system for peripheral nerve stimulation of claim 4, wherein after the step of leading the positive and negative electrodes of the μ LED out by using a gold wire, and before the step of winding the gold wire to form a spiral gold wire, the gold wire is packaged by using parylene, and then a second polydimethylsiloxane is coated on the wound spiral gold wire to form the lead wire.
6. The optogenetic system for peripheral nerve stimulation of claim 1, wherein a low power consumption bluetooth chip, a passive crystal oscillator, a ceramic patch antenna are disposed on the terminal circuit board, and the terminal circuit board communicates with the upper computer through the ceramic patch antenna.
7. The optogenetic system for peripheral nerve stimulation of claim 6, wherein the Bluetooth low energy chip comprises a plurality of output pins connected to the μ LED for individual control of parameters and status feedback of the μ LED.
8. The optogenetic system of claim 1, wherein the battery is removably coupled to the terminal circuit board in a pin header-to-pin header style, the pin header being coupled to the terminal circuit board, the pin header being coupled to the battery.
9. The optogenetic system for peripheral nerve stimulation of claim 1, wherein when the battery is mounted independently of the terminal circuit board, the battery is detachably connected to the terminal circuit board in a flexible wire type, and both ends of the flexible wire are connected to the battery and the terminal circuit board, respectively.
10. The optogenetic system of claim 1, wherein the optostimulation module is implanted at a site to be stimulated, and wherein the optostimulation module is configured to select the shape corresponding to a fixation material according to a shape of the site during the process of regulating the neuron activity at the site, wherein the fixation material comprises a self-curling material and a tissue glue.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211485233.6A CN115721869A (en) | 2022-11-24 | 2022-11-24 | Optogenetic system for peripheral nerve stimulation |
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| CN202211485233.6A CN115721869A (en) | 2022-11-24 | 2022-11-24 | Optogenetic system for peripheral nerve stimulation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117224859A (en) * | 2023-11-14 | 2023-12-15 | 浙江大学 | Anxiety state evaluation and multi-target time sequence optical stimulation and imaging system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117224859A (en) * | 2023-11-14 | 2023-12-15 | 浙江大学 | Anxiety state evaluation and multi-target time sequence optical stimulation and imaging system |
| CN117224859B (en) * | 2023-11-14 | 2024-02-06 | 浙江大学 | System comprising anxiety state assessment device and multi-target time-series optical stimulation and imaging device |
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