CN115721736A - Application of fluorescent dye in preparation of acupoint injection - Google Patents
Application of fluorescent dye in preparation of acupoint injection Download PDFInfo
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- CN115721736A CN115721736A CN202211337715.7A CN202211337715A CN115721736A CN 115721736 A CN115721736 A CN 115721736A CN 202211337715 A CN202211337715 A CN 202211337715A CN 115721736 A CN115721736 A CN 115721736A
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Abstract
The invention provides application of a fluorescent dye in preparing an acupoint injection, wherein the fluorescent dye comprises fluorescein sodium and/or indocyanine green. The invention provides the new application of the fluorescent dyes, has great significance for proving existence of channels and collaterals and disclosing structures of the channels and collaterals, and has promotion effect on popularization and development of Chinese theory and practice.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine channels and collaterals, in particular to application of a fluorescent dye in preparation of an acupoint injection.
Background
Meridians and collaterals and acupoints are the important concepts in traditional Chinese medicine. However, the meridians and acupoints are described more in terms of functions, and there is no uniform understanding on their structures, biological properties and physical properties. Various means are still being tried to observe and verify their presence.
Disclosure of Invention
In view of the above, the invention provides the application of fluorescent dye in the preparation of acupoint injection, aiming at proving the existence of meridians and collaterals and disclosing the structure of meridians and collaterals.
The invention provides application of a fluorescent dye in preparation of an acupoint injection, wherein the fluorescent dye comprises fluorescein sodium and/or indocyanine green.
Further, the injection volume of the fluorescent dye is 0.01-0.5ml, the concentration of fluorescein sodium is 2-20%, and the concentration of indocyanine green is 0.01-5%.
Further, the injection volume of the fluorescent dye is 0.05-0.2ml, the concentration of the fluorescein sodium is 5-15%, and the concentration of the indocyanine green is 0.1-2%.
Further, the injection volume of the fluorescent dye was 0.1ml, the injection concentration of fluorescein sodium was 10%, and the injection concentration of indocyanine green was 0.5%.
Further, fluorescent dyes include sodium fluorescein and indocyanine green.
Further, the acupoint injection comprises one or more of fluorescein sodium solution, indocyanine green solution and fluorescein sodium-indocyanine green mixed solution.
Further, the acupoint injection includes any one of human pericardial channel acupoint injection, human heart channel acupoint injection, human lung channel acupoint injection, human small intestine channel acupoint injection, human large intestine channel acupoint injection, human triple energizer channel acupoint injection, human liver channel acupoint injection, human spleen channel acupoint injection, human kidney channel acupoint injection, human gallbladder channel acupoint injection, human bladder channel acupoint injection and human stomach channel acupoint injection.
Furthermore, the injection for human pericardial meridian points comprises an injection for human pericardial meridian points of Neiguan points.
The invention also provides application of the fluorescent dye in preparing the acupoint injection for displaying meridian lines.
The invention also provides the application of the fluorescent dye in preparing the human body acupuncture point injection for displaying human body meridians.
The invention provides the new application of the fluorescent dyes, has great significance for proving existence of channels and collaterals and disclosing structures of the channels and collaterals, and has promotion effect on popularization and development of Chinese theory and practice.
Drawings
FIG. 1 is a schematic diagram of the present invention showing the labeling of acupoints when the pericardium meridian of a human body is used as the meridian to be studied;
FIG. 2 is a fluorescent line of the present invention after injection of fluorescein sodium at Neiguan acupoint in the pericardium channel of a human body;
FIG. 3 is a schematic diagram of the process of the present invention of pasting metal lines on the surface of the human body and the overlapping position of the fluorescent lines and performing longitudinal (up) or transverse (down) scanning ultrasonic imaging;
FIG. 4 is a signal of a metal wire (fluorescence wire) and tissues therebelow obtained after longitudinal (left) or transverse (right) ultrasonic scanning is performed after the metal wire is pasted on the fluorescence wire running along the pericardium in the present invention;
fig. 5 shows signals of a metal wire and a blood vessel below the metal wire obtained after longitudinal (left) or transverse (right) ultrasonic scanning is performed after the metal wire is pasted on a blood vessel of an arm in the invention.
Detailed Description
While the following is a description of the preferred embodiments of the present invention, it should be noted that various changes and modifications can be made by those skilled in the art without departing from the principle of the present invention, and these changes and modifications are also considered to be within the scope of the present invention.
The embodiment of the invention provides application of fluorescent dye in preparing an acupoint injection, and the acupoint injection can be used for displaying meridians. The fluorescent dye comprises fluorescein sodium and/or indocyanine green, namely, the fluorescent dye can be fluorescein sodium, indocyanine green and a mixture of the fluorescein sodium and the indocyanine green.
Sodium Fluorescein (molecular weight 376 Da) and Indocyanine Green (ICG, molecular weight 775 Da) are two hydrophilic fluorescent substances commonly used clinically as angiofluorescent contrast agents (Meira, J.et al. Imaging Reactions to fluorescence and Indocyanine Green in diagnostic: review of lipid and Proposal for patent's evaluation. Clin opthalmol.2020; 14: 171-178.). ICG has also been used in the surgical treatment of tumors to identify sentinel nodes (Polom, K.et. Current nodes and organizing future of individual green usage in surgery and oncology: a saline review. "Cancer 2011 117. Under the excitation of blue light, fluorescein sodium can emit yellow green fluorescence of about 535 nm; the ICG has a maximum absorption of 600-900 nm and an excited fluorescence wavelength of 750-950 nm. These fluorescences with longer wavelengths have a stronger ability to penetrate the skin, so they can be used as visualizations of the human meridians.
The CAS number of the fluorescein sodium adopted in the embodiment of the invention is 518-47-8, and the CAS number of the indocyanine green is 3599-32-4. They are clinically used vascular fluorescent contrast agents, and their small molecular weight and negative charge facilitate cell-to-cell movement in tissues. Their presence and migration paths can be determined by observing their fluorescence under corresponding excitation light illumination. In addition, the safety of the two substances is clinically proven, so that the two fluorescent dyes have obvious advantages in visualization of human meridians.
The injection volume of the fluorescent dye is 0.01-0.5ml, and intradermal injection is adopted. Preferably, the injection volume of the fluorescent dye is 0.05-0.2ml. Further preferably, the injection volume of the fluorescent dye is 0.1ml.
The concentration of fluorescein sodium is 2-20%. Preferably, the concentration of sodium fluorescein is 5-15%. Further preferably, the concentration of fluorescein sodium is 10%.
The concentration of the indocyanine green is 0.01-5%. Preferably, the concentration of indocyanine green is 0.1-2%. Further preferably, the concentration of indocyanine green is 0.5%.
The acupoint injection comprises one or more of a fluorescein sodium solution, an indocyanine green solution and a fluorescein sodium-indocyanine green mixed solution, namely, the acupoint injection can be the fluorescein sodium solution, the indocyanine green solution or the fluorescein sodium-indocyanine green mixed solution.
The injection can be used for displaying the channels and collaterals of human body meridian. Wherein, the acupuncture points can be acupuncture points on the following channels and collaterals, such as: the pericardium channel, lung channel of hand taiyin, large intestine channel of hand yangming, stomach channel of foot yangming, spleen channel of foot taiyin, heart channel of hand shaoyin, small intestine channel of hand taiyang, bladder channel of foot taiyang, kidney channel of foot shaoyin, pericardium channel of hand jueyin, trifocal channel of hand shaoyin, gallbladder channel of foot shaoyin and liver channel of foot jueyin, of course, other channels and collaterals can be used.
Specifically, the human body acupoint injection can be any one of human body acupoint injection for covering channels of the heart, human body lung channel acupoint injection, human body acupoint injection for small intestine channels, human body acupoint injection for large intestine channels, human body acupoint injection for triple energizer channels, human body acupoint injection for liver channels, human body acupoint injection for spleen channels, human body acupoint injection for kidney channels, human body acupoint injection for gallbladder channels, human body acupoint injection for bladder channels and human body acupoint injection for stomach channels.
For the above injection, the injection at the point of pericardium channel of human body is taken as an example, and the injection can be applied to the point of pericardium channel of human body to show the meridian line of pericardium channel of human body. The same principle of the injection is applied to the meridians and acupoints of human body, which is not repeated herein.
In addition, the injection for the acupuncture points of the cardiac meridian, the lung meridian, the small intestine meridian, the large intestine meridian and the triple energizer meridian is further described, and the injection for the acupuncture points of the pericardium meridian is taken as an example and relates to a plurality of injections, and the injections respectively correspond to the acupuncture points of the pericardium meridian on the small arm of the human body. The rest human meridian and acupoint injections also relate to a plurality of injections respectively, and the various injections respectively correspond to the acupoints of meridians and collaterals on the small arms of the human body, and are not described in detail.
The injection for the liver meridian point of the human body, the spleen meridian point of the human body, the kidney meridian point of the human body, the gallbladder meridian point of the human body, the bladder meridian point of the human body and the stomach meridian point of the human body is further explained, the injection for the liver meridian point of the human body is taken as an example and relates to a plurality of injections, and the injections respectively correspond to the pericardium channel on each point of the shank of the human body. The rest human meridian and acupoint injections also relate to a plurality of injections respectively, and the various injections respectively correspond to all acupoints of meridians on human shanks, and are not described in detail.
More specifically, the injection at the point of human pericardium channel can be an injection at the point of human pericardium channel and Neiguan, which is injected to the point of human pericardium channel and Neiguan on the forearm of human body and can be used to display the meridian line of human pericardium channel.
Further, the embodiment of the invention also provides application of the fluorescent dye in preparing the acupuncture point injection for displaying meridian lines.
Furthermore, the embodiment of the invention provides the application of the fluorescent dye in preparing the human body acupuncture point injection for displaying human body meridian lines.
The application of the fluorescent dye in preparing the human body acupoint injection for displaying the human body meridian line is verified as follows, taking the human body pericardium as the meridian to be researched and taking the injection of fluorescein sodium at the point of the Neiguan as an example:
step S1, according to the acupuncture and moxibustion acupoint location national standard (GB 12346-2006), points on the arm, such as daling, cicleft, cleft and quze, are marked, see fig. 1.
Step S2, injecting 0.05ml of 10% fluorescein sodium solution for injection and 0.05ml of 0.5% indocyanine green solution for injection into the Neiguan acupoint intradermally, then utilizing the excitation light with the wavelength of 488nm and the excitation light with the wavelength of 780nm, and observing and recording the fluorescence signal by adopting a filter only capable of passing light with the wavelength of more than 500nm and a filter only capable of passing light with the wavelength of more than 850nm, referring to the graph 2. As can be seen from fig. 2: a yellow fluorescent line appears between Neiguan point and Quze point, and the direction of the fluorescent line is consistent with that of the pericardium channel.
And S3, drawing out a fluorescent line on an arm by using a marker pen under laser, covering the fluorescent line with a metal line, and scanning the fluorescent line along the metal line in a transverse direction (the long side of the probe is vertical to the fluorescent line) and a longitudinal direction (the long side of the probe is parallel to the fluorescent line) by using an ultrasonic image to obtain a scanning picture and video data of the metal line and a blood flow signal below the metal line, wherein the reference is provided with figures 3 and 4. It should be noted that the signal of the metal wire in the ultrasound image may represent a fluorescence wire, which is at the position of depth 0 (skin surface) and appears white, which is obviously different from the blue or red signal of the blood vessel. As can be seen from fig. 4: no blood vessel image line appears under the metal line, no matter the longitudinal scan (left) or the transverse scan (right) under the fluorescence line.
Further, in order to verify that the blood flow signal is not observed due to the influence of the wire itself, the wire is adhered to a blood vessel on the surface of the arm skin for ultrasonic observation, and as a result, referring to fig. 5, it can be seen from fig. 5 that: red or blue blood flow signals appear under the wire, thereby indicating that the wire can display the location of the fluorescent line on the ultrasound image without affecting the observation of the blood flow, thereby further verifying that the fluorescent line is not emitted by the fluorescent substance entering the blood vessel.
In conclusion, the medical fluorescent dye is injected at the acupuncture points on the meridians of the human body, and then the ultrasonic imaging technology, the blood vessel imager or the blood vessel fluorescent marking technology are used for proving that the fluorescent lines diffused along the meridians are not formed by the flowing of the fluorescent dye in the blood vessels, so that the visualization of the meridians by using the fluorescent dye is realized. The invention has great significance for proving existence of channels and collaterals and revealing structures of the channels and collaterals, and has promotion effect on popularization and development of theory and practice of Chinese medicine.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The application of the fluorescent dye in preparing the acupoint injection is characterized in that,
the fluorescent dye comprises fluorescein sodium and/or indocyanine green.
2. The use according to claim 1,
the injection volume of the fluorescent dye is 0.01-0.5ml, the concentration of the fluorescein sodium is 2-20%, and the concentration of the indocyanine green is 0.01-5%.
3. The use according to claim 2,
the injection volume of the fluorescent dye is 0.05-0.2ml, the concentration of the fluorescein sodium is 5-15%, and the concentration of the indocyanine green is 0.1-2%.
4. The use according to claim 3,
the injection volume of the fluorescent dye is 0.1ml, the injection concentration of the fluorescein sodium is 10%, and the injection concentration of the indocyanine green is 0.5%.
5. The use according to claim 1,
the fluorescent dye comprises fluorescein sodium and indocyanine green.
6. The use according to claim 1,
the acupoint injection comprises one or more of a fluorescein sodium solution, an indocyanine green solution and a fluorescein sodium-indocyanine green mixed solution.
7. The use according to claim 1,
the acupoint injection comprises any one of human pericardial channel acupoint injection, human cardiac channel acupoint injection, human lung channel acupoint injection, human small intestine channel acupoint injection, human large intestine channel acupoint injection, human triple warmer acupoint injection, human liver channel acupoint injection, human spleen channel acupoint injection, human kidney channel acupoint injection, human gallbladder channel acupoint injection, human bladder channel acupoint injection and human stomach channel acupoint injection.
8. The use according to claim 7,
the injection for human pericardium acupoint comprises injection for human pericardium acupoint Neiguan acupoint.
9. The application of fluorescent dye in preparing acupoint injection for displaying meridian lines.
10. The application of fluorescent dye in preparing injection for acupuncture points of human body for displaying human body meridian lines.
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| CN202211337715.7A CN115721736A (en) | 2022-10-28 | 2022-10-28 | Application of fluorescent dye in preparation of acupoint injection |
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| CN202211337715.7A CN115721736A (en) | 2022-10-28 | 2022-10-28 | Application of fluorescent dye in preparation of acupoint injection |
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