CN115718027A - Micro fossil permanent mounting piece and manufacturing method thereof - Google Patents
Micro fossil permanent mounting piece and manufacturing method thereof Download PDFInfo
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- CN115718027A CN115718027A CN202211504232.1A CN202211504232A CN115718027A CN 115718027 A CN115718027 A CN 115718027A CN 202211504232 A CN202211504232 A CN 202211504232A CN 115718027 A CN115718027 A CN 115718027A
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- ultraviolet curing
- fossil
- curing glue
- specimen
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 32
- 239000003292 glue Substances 0.000 claims abstract description 87
- 239000011521 glass Substances 0.000 claims description 37
- 238000000034 method Methods 0.000 claims description 26
- 239000006059 cover glass Substances 0.000 claims description 16
- 229910021487 silica fume Inorganic materials 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 7
- 238000000576 coating method Methods 0.000 claims description 7
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 5
- 239000000853 adhesive Substances 0.000 claims description 5
- 230000001070 adhesive effect Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000005253 cladding Methods 0.000 claims 1
- 238000005286 illumination Methods 0.000 claims 1
- 210000001589 microsome Anatomy 0.000 abstract description 8
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 2
- 230000003685 thermal hair damage Effects 0.000 abstract description 2
- 238000001723 curing Methods 0.000 description 70
- 230000000694 effects Effects 0.000 description 6
- 230000003228 microsomal effect Effects 0.000 description 5
- 238000003825 pressing Methods 0.000 description 3
- 238000003848 UV Light-Curing Methods 0.000 description 2
- 239000002313 adhesive film Substances 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 241000206761 Bacillariophyta Species 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000243142 Porifera Species 0.000 description 1
- 206010047531 Visual acuity reduced Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000003010 ionic group Chemical group 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
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Abstract
The invention discloses a microsome fossil permanent mounting piece and a manufacturing method thereof. The manufacturing method is simple to operate, and glue leakage and air bubble generation are not easy to occur; the ultraviolet light is single-wavelength cold light, so that the thermal damage to the specimen is greatly reduced, the heating process is well controlled, and the consumed time is short; after being cured, the ultraviolet curing glue has no bubbles, good definition, stable performance, moisture resistance, high temperature resistance and high humidity resistance, and is beneficial to the storage of permanently mounted chips.
Description
Technical Field
The invention belongs to the field of specimen preparation, and relates to a microsomal fossil permanent mounting piece and a preparation method thereof.
Background
The micro fossil is a tiny corpse and a vestige of ancient organisms deposited in stratums during various geological history periods, and is a main research object of micro ancient biology. The microsomites can be used as important basis for stratigraphic division and geological research in stratigraphy, and can also be used as a mark for deducing ancient environment and recovering ancient geography. The microsomites are generally millimeter-sized fossils, such as fossils of porifera, radioactively, flagellates, spores, pollen, diatoms, and the like. Therefore, studies, including the description, identification and classification of microsomes, must be conducted by microscopy, even electron microscopy.
The traditional permanent tablet filling method of microsomes fossil is a method for filling tablets by heating gum. The method is difficult to control in operation, and particularly, the glue is easy to drip in a glue dripping process to cause glue leakage; the heating process is not easy to control, and the gum is easy to blacken; moreover, the tabletting takes long time, the gum needs to be placed in a ventilated environment for a long time and cannot be observed in time; in addition, the permanent tablet made of the gum has too many bubbles to cause the poor vision.
Disclosure of Invention
Aiming at the defects of the existing method, the invention provides the microsome fossil permanent mounting piece and the manufacturing method thereof, and the manufacturing method has simple operation and is not easy to leak glue and generate bubbles; the ultraviolet light is single-wavelength cold light, so that the thermal damage to the specimen is greatly reduced, the heating process is well controlled, and the consumed time is short; after being cured, the ultraviolet curing glue has no bubbles, good definition, stable performance, moisture resistance, high temperature resistance and high humidity resistance, and is beneficial to the storage of permanently mounted chips.
The invention adopts the following technical scheme:
the method for manufacturing the permanent mounting of the microsomal fossil comprises the steps of coating a specimen by using ultraviolet curing glue, and then irradiating the specimen by ultraviolet light to cure the ultraviolet curing glue, thereby obtaining the permanent mounting of the microsomal fossil.
On the basis of the technical scheme, the ultraviolet curing glue adopted by the manufacturing method of the micro fossil permanent mounting piece is transparent before and after curing.
On the basis of the technical scheme, the ultraviolet curing glue adopted by the manufacturing method of the micro fossil permanent mounting piece is a single-component acrylate adhesive.
On the basis of the technical scheme, the viscosity of the ultraviolet curing glue used in the manufacturing method of the micro fossil permanent mounting piece is 200-250 mPa.s.
On the basis of the technical scheme, the surface drying time of the ultraviolet curing glue used in the manufacturing method of the micro fossil permanent mounting piece is 10-20 s.
On the basis of the technical scheme, in the manufacturing method of the microsome fossil permanent mounting, the ultraviolet light irradiation is provided by the following steps: and (3) placing the ultraviolet curing glue coated specimen in an ultraviolet curing box for ultraviolet irradiation for 20-30 seconds.
On the basis of the technical scheme, the method for manufacturing the microsilica fossil permanent mounting piece, provided by the invention, by coating the specimen with the ultraviolet curing glue comprises the following steps: and placing the specimen on a glass slide, dripping ultraviolet curing glue on the glass slide, and covering the glass slide to ensure that the ultraviolet curing glue is fully contacted with the specimen and the glass slide.
On the basis of the technical scheme, the step of dripping ultraviolet curing glue on the glass slide and then covering the glass slide with a cover glass comprises the following steps: dripping 1 drop of ultraviolet curing glue on a glass slide on which a specimen is placed, slightly shaking the glass slide, and controlling the ultraviolet curing glue to enable the ultraviolet curing glue to be paved on the whole specimen; the coverslip was then grasped with forceps so that one side of the coverslip first contacted the droplet on the slide and then slowly leveled.
The invention also provides a microsomized stone permanent mounting piece which comprises a specimen and a specimen fixing agent for coating the specimen, wherein the specimen fixing agent is formed by curing ultraviolet curing glue.
On the basis of the technical scheme, a cover glass and a glass slide are respectively arranged on two sides of the specimen fixing agent for permanent microtome fossil mounting.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) In the method for manufacturing the micro fossil permanently-mounted chip, the ultraviolet curing glue is initially cured for 1-5 seconds under the irradiation of ultraviolet rays, and bonding can be completed within 20-30 seconds. Higher intensity can be achieved after irradiation. The method is simple and quick to operate, does not need to be placed and dried, can be directly observed after irradiation, saves time and labor, can be used for observing under a microscope after only 1-2 minutes is needed for manufacturing a permanent mounting piece by using the ultraviolet curing glue, and greatly saves the piece-making period by using the ultraviolet curing glue because the neutral gum needs 1-2 days.
(2) The method for manufacturing the permanent micro fossil mounting can quickly and agilely drip glue and mount a mounting, avoids most of bubbles, and ensures that the manufactured mounting specimen is completely fused with the glue, almost no bubbles exist, the image is very clear, and the observation effect is excellent. The manual mounting generally requires a moderate glue concentration for mounting, because the glue concentration affects the quality of the finished product, for example, when neutral gum is used, the gum is sticky and dropped on the glass slide, and the glue is not easy to scatter, and the glue is uneven and easy to generate large bubbles, which is difficult to remove, and the cover glass is easy to deviate from the central point in the process of removing the bubbles by pressing, which affects the observation effect.
(3) When the microsilica fossil permanent mounting provided by the invention is observed by using a high-magnification microscope, the permanent mounting made of the ultraviolet curing glue has strong light transmittance, the observation visual field of microsilica fossil specimens is clear, no air bubbles exist, and the effect is excellent.
Drawings
The invention will be further described with reference to the accompanying drawings, to which, however, the invention is not limited in any way.
FIG. 1 is a schematic diagram of a method for manufacturing a permanently installed microsilica fossil plate according to the present invention;
FIG. 2 is a schematic view of UV-curable glue, and number 1 is the mouth of a dropping bottle (caliber 0.5mm, one drop 0.02 mL).
Fig. 3 is a schematic view of the permanent mounting of microsilica fossil.
Fig. 4 is a schematic view of an ultraviolet curing box used in the present invention, in which numeral 2 is an ultraviolet irradiation countdown, and numeral 3 is a drawer type curing box.
Detailed Description
In order that the invention may be more readily understood, specific embodiments thereof will be described further below.
The method for manufacturing the microsome fossil permanent mounting piece provided by the invention adopts ultraviolet curing glue to coat a specimen, and provides ultraviolet irradiation to cure the ultraviolet curing glue, so that the microsome fossil permanent mounting piece is obtained.
On the basis of the technical scheme, in the manufacturing method of the permanent micro fossil mounting, the method for coating the specimen by using the ultraviolet curing glue comprises the following steps: placing the specimen on a glass slide, dripping ultraviolet curing glue on the glass slide, and then covering a cover glass to ensure that the ultraviolet curing glue is fully contacted with the specimen and the glass slide.
On the basis of the technical scheme, the step of covering a cover glass after ultraviolet curing glue is dripped on the glass slide comprises the following steps: dripping 1 drop of ultraviolet curing glue on a glass slide on which a specimen is placed, slightly shaking the glass slide, and controlling the ultraviolet curing glue to enable the ultraviolet curing glue to be paved on the whole specimen; the coverslip was then grasped with forceps so that one side of the coverslip first contacted the droplet on the slide and then slowly leveled off.
The method for manufacturing the micro fossil permanently-mounted slice comprises the following steps:
step one, glue dripping: dripping 1 drop of ultraviolet curing glue on a glass slide for placing a specimen, slightly shaking the glass slide, and controlling the ultraviolet curing glue to enable the ultraviolet curing glue to pave the whole specimen, wherein the specimen is fully contacted with the ultraviolet curing glue in the whole process, and no bubbles are generated.
Step two, sealing the sheet: the coverslip was grasped with forceps so that one side of the coverslip first contacted the droplet on the slide and then slowly leveled. The purpose is to prevent bubbles from appearing under the cover slip. And slightly pressing the cover glass by using a glass rod to ensure that the glass slide, the ultraviolet curing glue and the cover glass are fully contacted.
Thirdly, fixing: and (4) putting the prepared mounted sheet into an ultraviolet curing box, and setting the time for 30 seconds to obtain a fixed permanent mounted sheet.
On the basis of the technical scheme, the ultraviolet curing glue adopted by the manufacturing method of the micro-fossil permanent mounting piece is transparent before and after curing, and is preferably a single-component acrylate adhesive, and further has the viscosity of 200-250mPa & s and the surface drying time of 10-20 s.
The invention relates to selection of ultraviolet curing glue: the ultraviolet curing glue is prepared by adding a photoinitiator into resin with a special formula, and generating active free radicals or ionic groups after absorbing high-intensity ultraviolet light in an ultraviolet curing box, so that polymerization, crosslinking and grafting reactions are initiated, and the resin is converted from liquid to solid within seconds. The invention selects the colorless and transparent ultraviolet curing glue, and the observation of the specimen is not influenced after curing. Ultraviolet curing glue is generally divided into two major systems: acrylate systems and epoxy systems. Because of the many advantages of uv-curable glues: the epoxy resin has the advantages of excellent anti-yellowing, moisture and chemical properties, rapid curing, high production efficiency and the like. The invention comprehensively considers the bonding strength, curing time, durability and stability of the ultraviolet curing glue, and finally selects a single-component acrylate adhesive (UV 6017 material surface coating glue for UV curing). The glue has good adhesion to glass, the viscosity is 200-250mPa & s (25 ℃ rotary viscometer), the surface drying time is 15s, and the surface has no grease. Compared with other ultraviolet curing glue, the UV curing glue has the advantages of high transparency, moderate viscosity, proper curing time, no grease generation after curing, high stability and ideal effect in hundreds of experiments. The ultraviolet curing glue is a dilute glue, the glass slide can be paved by slightly shaking without bubbles, the thickness of the prepared micro fossil permanent mounting is only 10-20 mu m, the thickness of the glue film is not more than 0.2 mu m, and the effect is better when the thickness of the glue film is thinner. If the film thickness is too thick, it may not be focused when viewed with a high power lens, and the mount may be crushed.
On the basis of the technical scheme, in the manufacturing method of the microsome fossil permanent mounting, the ultraviolet light irradiation is provided by the following steps: and (3) placing the ultraviolet curing glue coated specimen in an ultraviolet curing box for ultraviolet irradiation for 20-30 seconds. The curing speed is very fast, and the flaking period is greatly saved.
The invention also provides a micro fossil permanent mounting piece which comprises a specimen and a specimen fixing agent for coating the specimen, wherein the specimen fixing agent is formed by curing ultraviolet curing glue.
On the basis of the technical scheme, a cover glass and a glass slide are respectively arranged on two sides of the specimen fixing agent for permanent microtome fossil mounting.
The technical solution of the present invention is further illustrated by the following specific examples:
example 1:
the embodiment provides a method for manufacturing a micro fossil permanent mounting plate, which comprises the following specific steps:
firstly, 1 drop of ultraviolet curing glue is dripped on a glass slide for placing a microsomal fossil specimen (extracted microsomal fossil such as sporopollen, holed worms, silicon-planted bodies and the like), the glass slide is gently shaken, the ultraviolet curing glue is controlled, the ultraviolet curing glue is paved on the whole specimen, and the specimen is fully contacted with the ultraviolet curing glue in the whole process without generating bubbles; then the cover glass is clamped by tweezers, so that one side of the cover glass is firstly contacted with the glue on the glass slide and then is slowly leveled. The purpose is to prevent bubbles from appearing under the cover slip. Slightly pressing the cover glass by using a glass rod to ensure that the glass slide, the ultraviolet curing glue and the cover glass are fully contacted; and finally, placing the manufactured packaged chip into an ultraviolet curing box, and setting the time for 30 seconds to obtain a fixed permanent packaged chip.
The specification of the glass slide used in this example is 25.4 × 76.2mm, the specification of the cover glass is 20 × 20mm, the dosage of the ultraviolet curing glue is 1 drop (0.02 mL) under the specification, and the ultraviolet curing time is 30 seconds. The power supply of the ultraviolet curing box is AC110V/22OV; rated current 3A; the working current is 1.5A; the lamp power is 30W; the optical effect power is 1200LM/W.
As shown in fig. 3, the thickness of the microsilica fossil permanent mounting adhesive film prepared in example 1 is not more than 0.2 μm, when observed by using a high-power microscope, the permanent mounting made of the ultraviolet curing adhesive has strong light transmittance, the observation field of the microsilica fossil specimen is clear, no air bubbles exist, and the effect is excellent. The hardness of the micro fossil permanent mounting adhesive film after hardening is 75 +/-2 (Shore D).
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (10)
1. A method for manufacturing micro fossil permanent mounting sheets is characterized in that: and after the specimen is coated by adopting the ultraviolet curing glue, ultraviolet irradiation is provided, so that the ultraviolet curing glue is cured, and the micro fossil permanent mounting is obtained.
2. The method for manufacturing permanently laminated microsilica fossil as claimed in claim 1, wherein: the ultraviolet curing glue is transparent before and after curing.
3. The method for manufacturing permanently laminated microsilica fossil as claimed in claim 1, wherein: the ultraviolet curing glue is a single-component acrylate adhesive.
4. The method for manufacturing permanently mounted microsilica fossil as claimed in claim 1, wherein: the viscosity of the ultraviolet curing glue is 200-250 mPa.s.
5. The method for manufacturing permanently mounted microsilica fossil as claimed in claim 1, wherein: the surface drying time of the ultraviolet curing glue is 10-20 s.
6. The method for manufacturing permanently laminated microsilica fossil as claimed in claim 1, wherein: the providing ultraviolet light illumination comprises: and (3) placing the ultraviolet curing glue coated specimen in an ultraviolet curing box for ultraviolet irradiation for 20-30 seconds.
7. The method for manufacturing permanently laminated microsilica fossil as claimed in claim 1, wherein: adopt ultraviolet curing glue cladding sample includes: placing the specimen on a glass slide, dripping ultraviolet curing glue on the glass slide, and then covering a cover glass to ensure that the ultraviolet curing glue is fully contacted with the specimen and the glass slide.
8. The method for manufacturing permanently laminated microsilica fossil as claimed in claim 1, wherein: the step of covering the glass slide with the cover glass after the ultraviolet curing glue is dripped on the glass slide comprises the following steps: dripping 1 drop of ultraviolet curing glue on a glass slide on which a specimen is placed, slightly shaking the glass slide, and controlling the ultraviolet curing glue to enable the ultraviolet curing glue to pave the whole specimen; the coverslip was then grasped with forceps so that one side of the coverslip first contacted the droplet on the slide and then slowly leveled off.
9. A micro fossil permanent mounting is characterized by comprising a specimen and a specimen fixing agent for coating the specimen, wherein the specimen fixing agent is formed by curing ultraviolet curing glue.
10. The permanently glazed microfossicle as recited in claim 9, wherein: and a cover glass and a glass slide are respectively arranged on two sides of the specimen fixing agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211504232.1A CN115718027A (en) | 2022-11-28 | 2022-11-28 | Micro fossil permanent mounting piece and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211504232.1A CN115718027A (en) | 2022-11-28 | 2022-11-28 | Micro fossil permanent mounting piece and manufacturing method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN115718027A true CN115718027A (en) | 2023-02-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211504232.1A Pending CN115718027A (en) | 2022-11-28 | 2022-11-28 | Micro fossil permanent mounting piece and manufacturing method thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345287A (en) * | 2019-12-31 | 2020-06-30 | 江西农业大学 | Kiwi fruit plant tissue solidified colloid specimen and manufacturing method thereof |
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2022
- 2022-11-28 CN CN202211504232.1A patent/CN115718027A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345287A (en) * | 2019-12-31 | 2020-06-30 | 江西农业大学 | Kiwi fruit plant tissue solidified colloid specimen and manufacturing method thereof |
Non-Patent Citations (3)
| Title |
|---|
| JEFFREY T. OSTERHOUT 等: "Deep-UV Raman Spectroscopy of Carbonaceous Precambrian Microfossils: Insights into the Search for Past Life on Mars", ASTROBIOLOGY, 3 October 2022 (2022-10-03) * |
| 董祯: "一种新型的水晶滴胶生成技术", 北京农业, 31 October 2015 (2015-10-31), pages 197 * |
| 高占林;李耀发;党志红;潘文亮;田士波;: "蚜虫玻片标本的制作方法与技巧", 河北农业科学, no. 03, 15 March 2009 (2009-03-15) * |
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