CN115702249A - 用于检测样品中的不同分析物以及分析物的不同亚组/变异体的多重方法 - Google Patents

用于检测样品中的不同分析物以及分析物的不同亚组/变异体的多重方法 Download PDF

Info

Publication number
CN115702249A
CN115702249A CN202180041893.2A CN202180041893A CN115702249A CN 115702249 A CN115702249 A CN 115702249A CN 202180041893 A CN202180041893 A CN 202180041893A CN 115702249 A CN115702249 A CN 115702249A
Authority
CN
China
Prior art keywords
analyte
oligonucleotides
specific
different
decoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180041893.2A
Other languages
English (en)
Chinese (zh)
Inventor
安德里亚斯·盖佩尔
弗兰克·赖内克
克里斯蒂安·科尔夫哈格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Analytical Bioscience Co ltd
Original Assignee
Analytical Bioscience Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2020/067010 external-priority patent/WO2020254519A1/en
Application filed by Analytical Bioscience Co ltd filed Critical Analytical Bioscience Co ltd
Publication of CN115702249A publication Critical patent/CN115702249A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN202180041893.2A 2020-06-18 2021-06-18 用于检测样品中的不同分析物以及分析物的不同亚组/变异体的多重方法 Pending CN115702249A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EPPCT/EP2020/067010 2020-06-18
PCT/EP2020/067010 WO2020254519A1 (en) 2019-06-18 2020-06-18 Method of signal encoding of analytes in a sample
US202063127910P 2020-12-18 2020-12-18
US63/127,910 2020-12-18
US202063129942P 2020-12-23 2020-12-23
US63/129,942 2020-12-23
PCT/EP2021/066668 WO2021255263A2 (en) 2020-06-18 2021-06-18 Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample

Publications (1)

Publication Number Publication Date
CN115702249A true CN115702249A (zh) 2023-02-14

Family

ID=79268536

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202180041893.2A Pending CN115702249A (zh) 2020-06-18 2021-06-18 用于检测样品中的不同分析物以及分析物的不同亚组/变异体的多重方法
CN202180041889.6A Pending CN115698322A (zh) 2020-06-18 2021-06-18 用于检测样品中的不同分析物的多重方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202180041889.6A Pending CN115698322A (zh) 2020-06-18 2021-06-18 用于检测样品中的不同分析物的多重方法

Country Status (8)

Country Link
US (4) US20240279736A1 (enExample)
EP (2) EP4168575A1 (enExample)
JP (2) JP7828901B2 (enExample)
KR (2) KR20230023647A (enExample)
CN (2) CN115702249A (enExample)
AU (2) AU2021294274A1 (enExample)
CA (2) CA3181725A1 (enExample)
WO (2) WO2021255244A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7828901B2 (ja) * 2020-06-18 2026-03-12 リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング 試料中の異なる分析物及び分析物の異なるサブグループ/変形物を検出するための多重的方法
DE102022131449A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131451A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131446A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131442A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Zuordnen von Bildbereichen einer Bildfolge zu Ergebnisklassen mittels eines Analytdaten-Auswertesystems mit einem Verarbeitungsmodells
DE102022131448A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131441A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131444A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131445A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
AU2024229355A1 (en) * 2023-03-01 2025-09-04 Resolve Biosciences Gmbh Complex formation for parallel detection of multiple analytes in multiple applications
WO2024256715A1 (en) * 2023-06-16 2024-12-19 Resolve Biosciences Gmbh Reagent for in situ hybridization quality control
DE102023122213A1 (de) 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen
WO2025093690A1 (en) 2023-11-02 2025-05-08 Resolve Biosciences Gmbh Method for preparing tissue sections
WO2025099313A1 (en) 2023-11-10 2025-05-15 Resolve Biosciences Gmbh Reducing background fluorescence in single molecule fluorescence in situ hybridization applications
WO2025125442A1 (en) * 2023-12-13 2025-06-19 Resolve Biosciences Gmbh High resolution multiplex method for detecting at least two targets
WO2025132546A1 (en) 2023-12-19 2025-06-26 Resolve Biosciences Gmbh Method for reducing nonspecific signals in in-situ hybridization assays
WO2025250686A1 (en) * 2024-05-28 2025-12-04 The Regents Of The University Of California Methods and systems for parallel spatially resolved single-cell profiling
WO2026037951A1 (en) 2024-08-16 2026-02-19 Resolve Biosciences Gmbh Compositions and methods for spatial analysis of multicell suspensions
WO2026068813A1 (en) 2024-09-30 2026-04-02 Resolve Biosciences Gmbh Immuno-oncology gene panel compositions and methods of making and use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017189525A1 (en) * 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1228811A (en) 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
WO2003016574A1 (en) 2001-08-16 2003-02-27 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
CA2480673A1 (en) 2002-03-29 2003-10-09 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
EP3042963A1 (en) 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US9677125B2 (en) 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
CN101895587B (zh) 2010-07-06 2015-09-16 中兴通讯股份有限公司 防止用户私自修改ip地址的方法、装置和系统
EP2794928B1 (en) 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
JP6096885B2 (ja) 2012-04-19 2017-03-15 シージーン アイエヌシー PTO切断及び伸長依存的なシグナリングオリゴヌクレオチド切断を用いたターゲット核酸配列の検出{DetectionofTargetNucleicAcidSequencebyPTOCleavageandExtension−DependentSignalingOligonucleotideCleavage}
US10510435B2 (en) * 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
KR102368591B1 (ko) 2013-04-30 2022-02-25 캘리포니아 인스티튜트 오브 테크놀로지 순차적 하이브리드화 바코딩에 의한 분자의 멀티플렉스 표지화
US20160369329A1 (en) 2013-04-30 2016-12-22 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers
US20150105298A1 (en) 2013-10-10 2015-04-16 The Research Foundation For The State University Of New York Multi-oligomer in situ hybridization probes
EP3169810B1 (en) 2014-07-17 2024-09-04 California Institute of Technology Multiplex analysis of molecules in single cells by image correlation
AU2015349870B2 (en) 2014-11-21 2019-09-26 Bruker Spatial Biology, Inc. Enzyme- and amplification-free sequencing
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
CN116200462B (zh) 2016-05-16 2025-10-03 布鲁克空间生物学公司 用于检测样品中目标核酸的方法
WO2018005336A1 (en) 2016-06-28 2018-01-04 Bristol-Myers Squibb Company Macrocyclic inhibitors of myeloperoxidase
CA3032649A1 (en) * 2016-08-01 2018-02-08 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
AU2017321492A1 (en) 2016-08-30 2019-03-14 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
AU2017361521B2 (en) 2016-11-21 2020-08-27 Bruker Spatial Biology, Inc. Chemical compositions and methods of using same
CN111699268B (zh) * 2017-12-08 2024-04-26 加州理工学院 通过用探针的快速切换和再杂交进行顺序杂交编条形码来多重标记分子
KR102893736B1 (ko) 2018-05-14 2025-11-28 브루커 스페이셜 바이올로지, 인크. 화학 조성물 및 이의 사용 방법
JP2022506518A (ja) 2018-11-01 2022-01-17 カリフォルニア インスティチュート オブ テクノロジー 高速広視野共焦点イメージング用のマルチチャネルラインスキャナ
US11421273B2 (en) 2018-12-03 2022-08-23 California Institute Of Technology In situ readout of DNA barcodes
CA3122569A1 (en) 2018-12-13 2020-06-18 President And Fellows Of Harvard College Amplification methods and systems for merfish and other applications
US20220128565A1 (en) 2019-02-04 2022-04-28 Akoya Biosciences, Inc. Analyte detection by selective labeling of biological samples
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
CA3146081A1 (en) 2019-07-30 2021-02-04 Huy Quoc NGUYEN Methods and compositions related to target analysis
CN114729391A (zh) 2019-09-30 2022-07-08 阿科亚生物科学股份有限公司 用酶介导的放大的多路复用成像
EP4065728A1 (en) 2019-11-25 2022-10-05 California Institute of Technology Quantitative analysis of single-cell proteins by identification and quantification separation (duet)
CA3158080A1 (en) 2019-11-26 2021-06-03 Huy Quoc NGUYEN Compositions, sets, and methods related to target analysis
CN115176027A (zh) 2019-12-12 2022-10-11 哈佛大学校长及研究员协会 用于光指导的生物分子条形码化的组合物和方法
WO2021247618A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Enrichment of nucleic acid sequences
JP7828901B2 (ja) * 2020-06-18 2026-03-12 リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング 試料中の異なる分析物及び分析物の異なるサブグループ/変形物を検出するための多重的方法
US20210395804A1 (en) 2020-06-19 2021-12-23 California Institute Of Technology Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017189525A1 (en) * 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARUNI P.K.K. KARUNANAYAKE MUDIYANSELAGE等: ""Second-generation" fluorogenic RNA-based sensors", 《METHODS》, vol. 161, 17 January 2019 (2019-01-17), pages 24 - 34 *
HARRY M.T.CHOI等: "Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust", 《DEVELOPMENT》, vol. 145, 26 June 2018 (2018-06-26), pages 165753 *
ZHINING SUN等: "Intracellular Imaging with Genetically Encoded RNA-Based Molecular Sensors", 《NANOMATERIALS》, vol. 9, 8 February 2019 (2019-02-08), pages 233 *

Also Published As

Publication number Publication date
CA3181725A1 (en) 2021-12-23
CN115698322A (zh) 2023-02-03
EP4168575A1 (en) 2023-04-26
US12442037B2 (en) 2025-10-14
JP2023529682A (ja) 2023-07-11
WO2021255263A2 (en) 2021-12-23
WO2021255244A1 (en) 2021-12-23
US20260234705A1 (en) 2026-08-13
US20240279736A1 (en) 2024-08-22
KR20230023647A (ko) 2023-02-17
AU2021291051A1 (en) 2022-12-08
CA3181737A1 (en) 2021-12-23
KR20230023646A (ko) 2023-02-17
JP2023530889A (ja) 2023-07-20
US12492427B2 (en) 2025-12-09
US20220205028A1 (en) 2022-06-30
US20230227907A1 (en) 2023-07-20
AU2021294274A1 (en) 2022-12-08
WO2021255263A3 (en) 2022-02-03
EP4168576A2 (en) 2023-04-26
JP7828901B2 (ja) 2026-03-12

Similar Documents

Publication Publication Date Title
JP7828901B2 (ja) 試料中の異なる分析物及び分析物の異なるサブグループ/変形物を検出するための多重的方法
JP7804465B2 (ja) 試料中の分析物のシグナルコード化方法
JP7811197B2 (ja) 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
US20250376718A1 (en) High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
US20240158836A1 (en) High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
US20100029492A1 (en) Nucleic acid chip for obtaining binding profile of single strand nucleic acid and unknown biomolecule, manufacturing method thereof and analysis method of unknown biomolecule using nucleic acid chip
US20240417781A1 (en) High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
CN1782096B (zh) 利用zip编码的pna芯片及其制备方法
JP2026507142A (ja) 多重用途における多重分析物の並行検出のための複合体形成
WO2025125442A1 (en) High resolution multiplex method for detecting at least two targets
HK40116450A (en) Array and detection method for detecting nucleic acid spatial information
HK40116511A (en) Array and detection method for detecting nucleic acid spatial information
HK40117219A (en) Array and detection method for detecting nucleic acid spatial information
HK40055932B (en) Single-channel sequencing method based on self-luminescence

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination