CA3181725A1 - Multiplex method for detecting different analytes in a sample - Google Patents
Multiplex method for detecting different analytes in a sample Download PDFInfo
- Publication number
- CA3181725A1 CA3181725A1 CA3181725A CA3181725A CA3181725A1 CA 3181725 A1 CA3181725 A1 CA 3181725A1 CA 3181725 A CA3181725 A CA 3181725A CA 3181725 A CA3181725 A CA 3181725A CA 3181725 A1 CA3181725 A1 CA 3181725A1
- Authority
- CA
- Canada
- Prior art keywords
- analyte
- signal
- oligonucleotides
- decoding
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EPPCT/EP2020/067010 | 2020-06-18 | ||
| PCT/EP2020/067010 WO2020254519A1 (en) | 2019-06-18 | 2020-06-18 | Method of signal encoding of analytes in a sample |
| US202063127910P | 2020-12-18 | 2020-12-18 | |
| US63/127,910 | 2020-12-18 | ||
| US202063129942P | 2020-12-23 | 2020-12-23 | |
| US63/129,942 | 2020-12-23 | ||
| PCT/EP2021/066620 WO2021255244A1 (en) | 2020-06-18 | 2021-06-18 | Multiplex method for detecting different analytes in a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3181725A1 true CA3181725A1 (en) | 2021-12-23 |
Family
ID=79268536
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3181725A Pending CA3181725A1 (en) | 2020-06-18 | 2021-06-18 | Multiplex method for detecting different analytes in a sample |
| CA3181737A Pending CA3181737A1 (en) | 2020-06-18 | 2021-06-18 | Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3181737A Pending CA3181737A1 (en) | 2020-06-18 | 2021-06-18 | Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US20240279736A1 (enExample) |
| EP (2) | EP4168575A1 (enExample) |
| JP (2) | JP7828901B2 (enExample) |
| KR (2) | KR20230023647A (enExample) |
| CN (2) | CN115702249A (enExample) |
| AU (2) | AU2021294274A1 (enExample) |
| CA (2) | CA3181725A1 (enExample) |
| WO (2) | WO2021255244A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7828901B2 (ja) * | 2020-06-18 | 2026-03-12 | リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング | 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法 |
| DE102022131449A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131447A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131451A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge |
| DE102022131446A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131442A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Zuordnen von Bildbereichen einer Bildfolge zu Ergebnisklassen mittels eines Analytdaten-Auswertesystems mit einem Verarbeitungsmodells |
| DE102022131448A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131441A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131450A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131444A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren zum Identifizieren von Analyten in einer Bildfolge |
| DE102022131445A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| AU2024229355A1 (en) * | 2023-03-01 | 2025-09-04 | Resolve Biosciences Gmbh | Complex formation for parallel detection of multiple analytes in multiple applications |
| WO2024256715A1 (en) * | 2023-06-16 | 2024-12-19 | Resolve Biosciences Gmbh | Reagent for in situ hybridization quality control |
| DE102023122213A1 (de) | 2023-08-18 | 2025-02-20 | Carl Zeiss Microscopy Gmbh | Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen |
| WO2025093690A1 (en) | 2023-11-02 | 2025-05-08 | Resolve Biosciences Gmbh | Method for preparing tissue sections |
| WO2025099313A1 (en) | 2023-11-10 | 2025-05-15 | Resolve Biosciences Gmbh | Reducing background fluorescence in single molecule fluorescence in situ hybridization applications |
| WO2025125442A1 (en) * | 2023-12-13 | 2025-06-19 | Resolve Biosciences Gmbh | High resolution multiplex method for detecting at least two targets |
| WO2025132546A1 (en) | 2023-12-19 | 2025-06-26 | Resolve Biosciences Gmbh | Method for reducing nonspecific signals in in-situ hybridization assays |
| WO2025250686A1 (en) * | 2024-05-28 | 2025-12-04 | The Regents Of The University Of California | Methods and systems for parallel spatially resolved single-cell profiling |
| WO2026037951A1 (en) | 2024-08-16 | 2026-02-19 | Resolve Biosciences Gmbh | Compositions and methods for spatial analysis of multicell suspensions |
| WO2026068813A1 (en) | 2024-09-30 | 2026-04-02 | Resolve Biosciences Gmbh | Immuno-oncology gene panel compositions and methods of making and use thereof |
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| CA1228811A (en) | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
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| KR102368591B1 (ko) | 2013-04-30 | 2022-02-25 | 캘리포니아 인스티튜트 오브 테크놀로지 | 순차적 하이브리드화 바코딩에 의한 분자의 멀티플렉스 표지화 |
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| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| JP2022506518A (ja) | 2018-11-01 | 2022-01-17 | カリフォルニア インスティチュート オブ テクノロジー | 高速広視野共焦点イメージング用のマルチチャネルラインスキャナ |
| US11421273B2 (en) | 2018-12-03 | 2022-08-23 | California Institute Of Technology | In situ readout of DNA barcodes |
| CA3122569A1 (en) | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
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| CN119220641A (zh) | 2019-05-31 | 2024-12-31 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| CA3146081A1 (en) | 2019-07-30 | 2021-02-04 | Huy Quoc NGUYEN | Methods and compositions related to target analysis |
| CN114729391A (zh) | 2019-09-30 | 2022-07-08 | 阿科亚生物科学股份有限公司 | 用酶介导的放大的多路复用成像 |
| EP4065728A1 (en) | 2019-11-25 | 2022-10-05 | California Institute of Technology | Quantitative analysis of single-cell proteins by identification and quantification separation (duet) |
| CA3158080A1 (en) | 2019-11-26 | 2021-06-03 | Huy Quoc NGUYEN | Compositions, sets, and methods related to target analysis |
| CN115176027A (zh) | 2019-12-12 | 2022-10-11 | 哈佛大学校长及研究员协会 | 用于光指导的生物分子条形码化的组合物和方法 |
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| JP7828901B2 (ja) * | 2020-06-18 | 2026-03-12 | リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング | 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法 |
| US20210395804A1 (en) | 2020-06-19 | 2021-12-23 | California Institute Of Technology | Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing |
-
2021
- 2021-06-18 JP JP2022575771A patent/JP7828901B2/ja active Active
- 2021-06-18 US US18/001,930 patent/US20240279736A1/en active Pending
- 2021-06-18 EP EP21732086.0A patent/EP4168575A1/en active Pending
- 2021-06-18 US US18/001,933 patent/US12492427B2/en active Active
- 2021-06-18 WO PCT/EP2021/066620 patent/WO2021255244A1/en not_active Ceased
- 2021-06-18 AU AU2021294274A patent/AU2021294274A1/en active Pending
- 2021-06-18 EP EP21733989.4A patent/EP4168576A2/en active Pending
- 2021-06-18 JP JP2022575762A patent/JP2023529682A/ja active Pending
- 2021-06-18 CN CN202180041893.2A patent/CN115702249A/zh active Pending
- 2021-06-18 KR KR1020227043398A patent/KR20230023647A/ko active Pending
- 2021-06-18 KR KR1020227043397A patent/KR20230023646A/ko active Pending
- 2021-06-18 CN CN202180041889.6A patent/CN115698322A/zh active Pending
- 2021-06-18 CA CA3181725A patent/CA3181725A1/en active Pending
- 2021-06-18 WO PCT/EP2021/066668 patent/WO2021255263A2/en not_active Ceased
- 2021-06-18 AU AU2021291051A patent/AU2021291051A1/en active Pending
- 2021-06-18 CA CA3181737A patent/CA3181737A1/en active Pending
- 2021-12-17 US US17/554,093 patent/US12442037B2/en active Active
-
2025
- 2025-11-03 US US19/377,307 patent/US20260234705A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN115702249A (zh) | 2023-02-14 |
| CN115698322A (zh) | 2023-02-03 |
| EP4168575A1 (en) | 2023-04-26 |
| US12442037B2 (en) | 2025-10-14 |
| JP2023529682A (ja) | 2023-07-11 |
| WO2021255263A2 (en) | 2021-12-23 |
| WO2021255244A1 (en) | 2021-12-23 |
| US20260234705A1 (en) | 2026-08-13 |
| US20240279736A1 (en) | 2024-08-22 |
| KR20230023647A (ko) | 2023-02-17 |
| AU2021291051A1 (en) | 2022-12-08 |
| CA3181737A1 (en) | 2021-12-23 |
| KR20230023646A (ko) | 2023-02-17 |
| JP2023530889A (ja) | 2023-07-20 |
| US12492427B2 (en) | 2025-12-09 |
| US20220205028A1 (en) | 2022-06-30 |
| US20230227907A1 (en) | 2023-07-20 |
| AU2021294274A1 (en) | 2022-12-08 |
| WO2021255263A3 (en) | 2022-02-03 |
| EP4168576A2 (en) | 2023-04-26 |
| JP7828901B2 (ja) | 2026-03-12 |
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