CN115684615A - Kit and POCT blood cell analyzer - Google Patents

Abstract

The application provides a kit and POCT blood cell analyzer. The kit comprises: the pipette comprises at least two pipette heads and a cartridge body, wherein the at least two pipette heads are used for sucking liquid, and the volumes of the at least two pipette heads are different; the box body is provided with at least two pipette head placing pools for placing at least two pipette heads. The reagent box of this application is through setting up the pipette head that two volumes are different to improve the application of sample precision, thereby improve the accuracy of reagent box testing result.

Description

Kit and POCT blood cell analyzer

Technical Field

The application relates to the technical field of medical instruments, in particular to a kit and a POCT blood cell analyzer.

Background

The blood cell analyzer is a commonly used medical detection device, is an instrument for detecting parameters such as the number, the proportion and the like of blood cells (red blood cells, white blood cells and blood platelets) in blood, and realizes functions such as microbial infection types, anemia diagnosis and treatment, blood disease diagnosis and the like of a detected sample through blood analysis. With the progress of technology and the development of science and technology, the function of the blood cell analyzer is continuously expanded, the performance is continuously improved, the automation degree is continuously improved, and the blood cell analyzer is widely applied clinically.

In the existing POCT blood cell analyzer, samples or reagents are generally added through a pipette tip, however, in the prior art, the sample adding precision of the pipette tip is low, and the accuracy of a sample detection result of a kit is not high.

Disclosure of Invention

The application provides a kit and POCT blood cell analyzer to the sample adding precision of straw head is lower among the solution prior art, and the sample testing result's of kit technical problem that the accuracy is not high.

In order to solve the technical problem, the application adopts a technical scheme that: providing a kit comprising: at least two pipette heads for aspirating liquids, the at least two pipette heads having different volumes; the box body is provided with at least two pipette head placing pools for placing at least two pipette heads.

Further, at least two pipette heads have different lengths and/or inner diameters, and at least two pipette head placement pools have different inner diameters so as to be respectively used for placing the at least two pipette heads.

Further, each pipette head comprises: a tube body formed with a liquid suction port; the fixing part is arranged at the periphery of the tube body and used for fixing the tube body to the pipette head placing pool, the fixing part is positioned at one end, away from the liquid suction port, of the tube body, the fixing part comprises at least two steps, the at least two steps are distributed at intervals towards one side away from the liquid suction port, and the outer diameters of the at least two steps are gradually increased towards the direction away from the liquid suction port.

Further, the inner wall of the pipette head is provided with a hydrophobic coating.

Further, the pipette head comprises a pipe body and fixing parts, wherein the fixing parts are fixed on the periphery of the pipe body and used for fixing the pipe body to the pipette head placing pool, and the fixing parts of at least two pipette heads are different in shape.

Further, the cartridge includes: the first box body is provided with at least two pipette head placing pools, and a first detection pool position used for electrical impedance detection; the second box body is provided with a second detection pool position, the second detection pool position is used for optical detection, and the first box body is detachably connected with the second box body.

Furthermore, one side of the first box body is provided with at least one clamping groove, and one side of the second box body is provided with a convex block matched with the clamping groove.

Furthermore, one side of the first box body is provided with a hanging rack, and at least two ends of the second box body are hung on the hanging rack.

In order to solve the above technical problem, another technical solution adopted by the present application is: the POCT hematology analyzer comprises a liquid transfer device and the reagent kit of any embodiment, wherein the end part of the liquid transfer device is used for being matched with a suction pipe head in the reagent kit to carry out liquid transfer operation.

Furthermore, a suction head is arranged at the end part of the liquid transfer device and used for being sleeved with the suction head, wherein the suction head is arranged in a step shape and used for adapting to the suction heads with different calibers.

The beneficial effect of this application is: be different from prior art's condition, the kit of this application includes two at least pipette heads and box body, and the box body is provided with two at least pipette heads and places the pond for place two at least pipette heads. In the kit that this application provided, the pipette head is used for absorbing liquid to the application of sample, the volume of two at least pipette heads is different, through this kind of mode, can improve the application of sample precision of kit, thereby improves the accuracy of the testing result of sample.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:

FIG. 1 is a schematic structural diagram of one embodiment of a kit provided herein;

FIG. 2 is an exploded schematic view of the kit shown in FIG. 1;

FIG. 3 is a schematic cross-sectional view of a perspective of the kit shown in FIG. 1;

FIG. 4 is a schematic structural view of an embodiment of the rear well body of the kit shown in FIG. 1;

FIG. 5 is a schematic structural view of another embodiment of the rear well body in the kit shown in FIG. 1;

FIG. 6 is a schematic structural view of another embodiment of a kit provided herein;

FIG. 7 is an exploded schematic view of the kit shown in FIG. 6;

FIG. 8 is a schematic structural diagram of another embodiment of a kit provided herein;

FIG. 9 is an exploded schematic view of the kit shown in FIG. 8;

FIG. 10 is a schematic structural view of another embodiment of a kit provided herein;

fig. 11 is a schematic structural diagram of an embodiment of a pipette and a pipette tip in the POCT hematology analyzer provided in the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that if directional indications (such as upper, lower, left, right, front, rear, 8230; \8230;) are referred to in the embodiments of the present application, the directional indications are only used for explaining the relative positional relationship between the components in a specific posture (as shown in the attached drawings), the motion situation, etc., and if the specific posture is changed, the directional indications are correspondingly changed.

In addition, if there is a description of "first", "second", etc. in the embodiments of the present application, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present application.

A first embodiment, which is illustrated in fig. 1-3, wherein fig. 1 is a schematic structural diagram of an embodiment of the kit provided herein, and fig. 2 is an exploded schematic diagram of the kit illustrated in fig. 1; fig. 3 is a schematic cross-sectional view of a perspective view of the reagent cartridge shown in fig. 1, the reagent cartridge comprising: a case 10, a gasket 20, a microporous sheet 30, and a rear tank body 40.

Specifically, as shown in fig. 1 and 2, the cartridge 10 includes a front pool 101 and a mounting chamber 102 communicating with the front pool 101. In this embodiment, the front pool 101 is an electrical impedance detection pool, and two sets of front pools are provided, and are respectively used for performing White Blood Cell (WBC) detection and Red Blood Cell (RBC) detection in cooperation. In other embodiments, the front pool 101 may also be provided with one group or at least three groups, etc. for detecting red blood cells, white blood cells, or other items, and the arrangement may be specifically set according to actual needs.

As shown in fig. 3, the gasket 20 is disposed in the mounting chamber 102 on a side of the mounting chamber 102 close to the front tank 101, the microporous sheet 30 is provided with micropores 31 for allowing cells to pass therethrough one by one, the microporous sheet 30 is disposed on a side of the gasket 20 away from the front tank 101, the rear tank body 40 is disposed on a side of the microporous sheet 30 away from the gasket 20, and the rear tank body 40 is used for fixing the microporous sheet 30 and the gasket 20 to the case 10. Rear cell body 40 is snap fit, threaded, interference, laser welded, or adhesively engaged with mounting cavity 102.

In this embodiment, the sealing ring 20, the microporous sheet 30 and the rear cell body 40 are sequentially disposed in the mounting cavity 102, and the microporous sheet 30 and the sealing ring 20 are pressed and fixed on the case 10 through the rear cell body 40, so that the inner end of the mounting cavity 102 is well sealed, the reliability of the reagent kit is improved, and the reagent kit is convenient to process and assemble. Here, the inner end of the installation cavity 102 refers to an end of the installation cavity 102 near the front pool 101.

As shown in fig. 3, box body 10 includes the via hole 103 of intercommunication forebay 101 and installation cavity 102, and sealing washer 20 is installed in via hole 103 department to with installation cavity 102 clearance fit, so, when sealing washer 20 put into, can not take place the slope, make things convenient for the installation of sealing washer 20, and the liquid of forebay 101 can not enter into in the back pond body 40 from the edge of sealing washer 20, improve the reliability that the kit detected.

Further, the ratio of the diameter of the microporous sheet 30 to the diameter of the via hole 103 is not less than 1.7, and the diameter of the microporous sheet 30 is not greater than the inner diameter of the mounting cavity 102, for example, the ratio of the diameter of the microporous sheet 30 to the diameter of the via hole 103 is 1.7, 1.75, 1.8, etc., and the diameter of the microporous sheet 30 may be equal to the inner diameter of the mounting cavity 102 or slightly smaller than the inner diameter of the mounting cavity 102. In this way, the rear tank body 40 can be conveniently pressed against the microporous sheet 30, liquid is prevented from entering the rear tank body 40 from the edge of the microporous sheet 30, the installation of the microporous sheet 30 is convenient, and the assembly time of the microporous sheet 30 is saved.

Alternatively, the cartridge 10, the gasket 20, the microporous sheet 30, and the rear cell body 40 are separate members, respectively. In one embodiment, the microporous sheet 30 and the gasket 20 are bonded to the cartridge 10, and the rear cell body 40 is bonded to the cartridge 10. In another embodiment, the microporous sheet 30 and the gasket 20 are bonded to the rear cell body 40, and the rear cell body 40 is bonded to the cartridge 10. Therefore, the assembling difficulty is reduced.

In other embodiments, the gasket 20, the microporous sheet 30, and the rear tank body 40 may be an integral structure to reduce the number of components, the assembly difficulty, and the assembly time.

The cartridge 10 and/or the rear pool body 40 may be plastic. The microporous sheet 30 may be a plastic sheet or a ceramic sheet, which has relatively low material cost, and may be a disposable product without using an expensive material that can be repeatedly cleaned and used. The sealing ring 20 may be injection molded using a relatively soft plastic material using a two-shot molding process.

Further, as shown in fig. 2 and 3, the cartridge 10 is provided with a front cell electrode 60 corresponding to the front cell 101, the rear cell body 40 is formed with a drainage chamber 41 (also referred to as a rear cell), and the front cell 101 and the drainage chamber 41 are communicated through the micropores 31. The rear cell body 40 is provided with a rear cell electrode 50 extending to the drainage chamber 41, and the front cell electrode 60 and the rear cell electrode 50 are respectively positioned at two sides of the microporous sheet 30 at intervals. The outer ends of the front cell electrode 60 and the rear cell electrode 50 (i.e., the two ends far away from each other) are used for connecting a working voltage, the inner ends of the front cell electrode 60 and the rear cell electrode 50 (i.e., the two ends near each other) are in contact with a sample liquid to be detected, the liquid level of the sample liquid to be detected in the front cell 101 is higher than that of the front cell electrode 60, and the drainage cavity 41 is filled with the sample liquid to be detected during detection.

In the embodiment of the application, the axis of the front cell electrode 60 and the axis of the rear cell electrode 50 are approximately on the same straight line, and experiments prove that the detection precision is relatively high when the axis of the front cell electrode 60 and the axis of the rear cell electrode 50 are coaxial. In other embodiments, the axis of the front cell electrode 60 and the axis of the rear cell electrode 50 may not be collinear.

In the impedance channel, during the calculation of the cells, the fluid in the front reservoir 101 passes through the microporous sheet 30, and the drainage lumen 41 (back reservoir) is slowly filled with fluid. The front cell electrode 60 and/or the rear cell electrode 50 can be cylindrical electrodes. However, in this manner, the liquid column passing through the pores 31 of the microporous sheet 30 may collide with the rear cell electrode 50, and then the backflow may impact the microporous sheet 30, which may affect the stability of the signal, and may generate M-wave, thereby reducing the reliability of the detection.

In order to improve the above problem, in some embodiments, the inner end surface of the rear cell electrode 50 may be configured to be convex, for example, the inner end of the rear cell electrode 50 may be configured to be hemispherical, so that the inner end surface of the rear cell electrode 50 is a curved surface, and thus, the liquid backflushing flow may be uniformly dispersed around from the middle, so that the backflushing flow may not directly flow to the microporous sheet 30, so as to reduce the phenomenon of cell particle backflushing. When the inner end surface is referred to the front cell 101, the end of the rear cell electrode 50 pointing to the front cell 101 is the inner end surface, and the end departing from the front cell 101 is the outer end surface.

Further, the rear cell body 40 is provided with a liquid outlet 44, the liquid outlet 44 is communicated with the installation cavity 102, and the liquid outlet 44 is used for discharging gas or liquid in the drainage cavity 41.

The distance between the inner end surface of the rear cell electrode 50 and the micro-porous sheet 30 is not less than 5mm, for example, the distance between the inner end surface of the rear cell electrode 50 and the micro-porous sheet 30 may be set to 5mm, 6mm, 7mm, 8mm, or the like. By limiting the distance relationship between the microporous sheet 30 and the inner end surface of the rear cell electrode 50, the microporous sheet 30 is prevented from being too close to the rear cell electrode 50, so as to give enough buffer distance to the liquid, and facilitate the liquid in the drainage cavity 41 to be led out from the liquid outlet 44 by negative pressure in time.

Further, the inner end of the mounting cavity 102 is communicated with the front pool 101 through a via hole 103, and the outer end of the mounting cavity 102 is an open end for receiving the rear pool body 40. The end surface of the mounting cavity 102 may be provided with a positioning portion (not shown), and the rear tank body 40 may be provided with a fitting portion (not shown), which is in fitting connection with the positioning portion, so as to position and mount the rear tank body 40.

In a specific embodiment, the outer end surface of the mounting cavity 102 may be provided with a positioning protrusion as a positioning portion, and the rear tank body 40 may be provided with a positioning groove adapted to the positioning protrusion as a matching portion, so that when the rear tank body 40 is mounted, the positioning protrusion is matched with the positioning groove to position the rear tank body 40. Therefore, the assembly difficulty of the rear pool body 40 can be reduced, and the assembly time can be saved.

In other embodiments, the outer end surface of the mounting cavity 102 may be provided with a positioning groove to serve as a positioning portion, and the rear tank body 40 is provided with a positioning protrusion adapted to the positioning groove to serve as a matching portion, and when the rear tank body 40 is mounted, the positioning protrusion is matched with the positioning groove to perform positioning mounting on the rear tank body 40. Thus, the assembly difficulty of the rear pool body 40 can be reduced, and the assembly time can be saved.

In conclusion, the kit of the embodiment has a simple structure, the fixing mode of the microporous sheet 30 is novel, the processing is convenient, the assembling difficulty is low, and the reliability is high.

A second embodiment, the present application further provides a kit, as shown in fig. 1 to 5, fig. 4 is a schematic structural diagram of an embodiment of a back pool body in the kit shown in fig. 1, fig. 5 is a schematic structural diagram of another embodiment of the back pool body in the kit shown in fig. 1, the kit of this embodiment includes an electrical impedance detection cell (not labeled in the figures) for performing electrical impedance detection on a sample to be detected, the electrical impedance detection cell includes a back pool body 40 and a back pool electrode 50, the back pool electrode 50 is embedded on the back pool body 40, and the back pool electrode 50 and the back pool body 40 can be integrally injection-molded or detachably connected.

The rear cell body 40 is provided with a drainage cavity 41, the rear cell electrode 50 is arranged on the rear cell body 40 and extends to the drainage cavity 41, the inner end face of the rear cell electrode 50 is a convex face, specifically, the inner end of the rear cell electrode 50 can be hemispherical, and the inner end face of the rear cell electrode 50 is a side end face of the rear cell electrode 50 extending to the drainage cavity 41. For the convex structure of the rear cell electrode 50, please refer to the description of the first embodiment, and the description thereof is omitted.

Further, as shown in fig. 4, the rear cell body 40 includes a bottom wall 43 and a side wall 42, the bottom wall 43 connecting the side wall 42 to form the drainage lumen 41, and the rear cell electrode 50 is disposed on the bottom wall 43. A liquid outlet 44 is formed in the side wall 42, and the liquid outlet 44 is communicated with the drainage cavity 41 for discharging the gas or liquid in the drainage cavity 41. In the embodiment shown in fig. 4, the bottom wall 43 is a plane, the plane of the bottom wall 43 is inclined with respect to the side wall 42, and the liquid outlet 44 is disposed at the intersection of the bottom wall 43 and the side wall 42 and on the side away from the microporous sheet 30. In this embodiment, the bottom wall 43 is set as an upward inclined plane and serves as a drainage surface, so that the liquid is drained from the bottom wall 43 and guided to the liquid outlet 44 after flowing to the rear cell electrode 50 through the microporous sheet 30, the liquid can conveniently flow out from the liquid outlet 44, the liquid accumulation is avoided, the signal disturbance caused by the convolution of particles near the rear cell electrode 50 is reduced, and the reliability of detection is improved.

In addition, the liquid outlet 44 is located at the intersection of the side wall 42 and the bottom wall 43, so that the end of the slope formed by the bottom wall 43 is close to the liquid outlet 44, and when the drainage of the liquid in the drainage cavity 41 through the bottom wall 43 is finished, the liquid directly flows out of the liquid outlet 44, further reducing the retention of the liquid.

In another embodiment, as shown in FIG. 5, the bottom wall 43 also serves as a drainage surface for draining fluid in the drainage lumen 41. Specifically, the distance from the bottom wall 43 to the microporous sheet 30 gradually decreases in the direction from the position where the bottom wall 43 is provided with the rear cell electrode 50 to the edge of the bottom wall 43. That is, the bottom wall 43 may be flared to drain the liquid in the drainage lumen 41.

Further, as shown in fig. 5, the liquid outlet 44 is disposed at the intersection of the bottom wall 43 and the side wall 42, that is, the liquid outlet 44 may be disposed close to the bottom wall 43, so that the liquid in the rear tank directly flows out of the liquid outlet 44 when the drainage of the liquid through the bottom wall 43 is finished, thereby reducing the retention of the liquid.

Further, the maximum distance value of the distance between the inner end surface of the rear cell electrode 50 and the bottom wall 43 is less than 0.5mm. The distance of the inner end surface of the rear cell electrode 50 protruding out of the inner surface of the bottom wall 43 is smaller, so that the liquid clamping phenomenon of the rear cell electrode 50 is reduced.

Further, the distance between the inner end surface of the rear cell electrode 50 and the microporous sheet 30 is not less than 5mm, so that the microporous sheet 30 is prevented from being too close to the rear cell electrode 50, a sufficient buffer distance is provided for liquid, and the liquid in the drainage cavity 41 can be conveniently led out from the liquid outlet 44 by negative pressure in time.

Optionally, the rear cell electrode 50 is disposed at the central position of the bottom wall 43, and the rear cell electrode 50 is disposed coaxially with the drainage cavity 41, so that the mold drawing operation after the mold is formed is facilitated.

In the above embodiment, the electrical impedance detection cell has a novel structure, the inner end surface of the rear cell electrode 50 is set to be a convex surface, and the bottom wall 43 of the rear cell body 40 forms a drainage surface to guide liquid to the liquid outlet 44.

In a third embodiment, the present application further provides a kit, please refer to fig. 6 and 7, fig. 6 is a schematic structural diagram of another embodiment of the kit provided in the present application, fig. 7 is an exploded schematic diagram of the kit shown in fig. 6, the kit includes a box body 10, the box body 10 includes a first box body 11 and a second box body 12, and the first box body 11 and the second box body 12 are detachably connected.

The first box body 11 is provided with a first detection cell position for electrical impedance detection, and the second box body 12 is provided with a second detection cell position for optical detection. In this embodiment, the first detection cell site may be used for WBC detection and RBC detection, the second detection cell site may be used for detection of a specific protein, and in other embodiments, the second detection cell site may also be used in combination with other biochemical detection, immunoassay, and the like.

In this embodiment, the first box 11 is detachably connected to the second box 12. In this manner, storage and transport of the cartridge 10 is facilitated. In other embodiments, the first container 11 and the second container 12 may be integrally formed to improve the reliability of the connection between the first container 11 and the second container 12.

In the embodiment shown in fig. 6 and 7, at least one locking groove 111 is formed on one side of the first container 11, and a protrusion 121 engaged with the locking groove 111 is formed on one side of the second container 12. The locking slot 111 cooperates with the protrusion 121 to hang the second container 12 on the first container 11. In this way, the connection structure of the first container 11 and the second container 12 is simple, and the assembling and disassembling processes are simple. Preferably, the number of the card slots 111 and the protrusions 121 may be provided in plural to make the connection of the second container 12 with the first container 11 more firm.

Further, one side of the card slot 111 far away from the first box 11 is set to be a necking, that is, the area of the opening at the side of the card slot 111 far away from the first box 11 is smaller than the area of the bottom wall of the card slot 111 near the side of the first box 11. Thus, the second box 12 can be effectively prevented from falling off from the first box 11.

Further, the cross-sectional shape of the card slot 111 along a first plane, which is a plane perpendicular to the thickness direction of the first container 11, may be trapezoidal. In this way, the connecting part of the first box body 11 and the second box body 12 has a regular shape, which facilitates the processing of the box body 10.

In another embodiment, as shown in fig. 8 and 9, fig. 8 is a schematic structural view of another embodiment of the reagent kit provided by the present application, and fig. 9 is an exploded schematic view of the reagent kit shown in fig. 8, specifically, a hanger 112 is disposed on one side of the first box 11, and an edge of the second box 12 is hung on the hanger 112.

Specifically, as shown in fig. 9, the hanging bracket 112 is formed with a hanging hole 1120, the second container 12 is inserted into the hanging hole 1120, and an outer end edge of the second container 12 is supported on the hanging bracket 112. In this way, the stress on the second box 12 can be more uniform, and the second box 12 can be more stably fixed on the hanger 112.

Further, the shape of the section of the hanging hole 1120 may be rectangular, circular, trapezoidal, triangular, or irregular, etc. The shape of the hanging hole 1120 may be adapted to the shape of the second container 12. For example, when the second container 12 has a rectangular shape, the hanging hole 1120 is also formed in a rectangular shape.

In other embodiments, at least two edges of the second container 12 are suspended from the hanger 112, so that the second container 12 is supported by the hanger 112. In this way, the structure of the box body 10 can be simplified, and the production is convenient. For example, the hanging rack 112 may directly include two or three support rods for supporting the edges of two or three ends of the second container 12, so that material cost can be saved.

Further, as shown in fig. 7 and 8, the first detection cell site may include two sets of front cells 101, and the two sets of front cells 101 may be configured to cooperate to perform white blood cell detection and red blood cell detection, respectively. The first detection cell site may further comprise at least one pipette tip placement cell 113, a diluent cell 114, a hemolytic agent cell 115 and a sample accommodation cell 116, wherein the pipette tip placement cell is configured to accommodate the pipette tip 60, the diluent cell 114 is configured to encapsulate a diluent, and the hemolytic agent cell 115 is configured to encapsulate a hemolytic agent; the first detection cell site may further comprise a sample dilution cell 117, the sample dilution cell 117 being for sample dilution. Wherein, a plurality of pond positions are the straight line and arrange, and this kind of mode can be convenient for liquid-transfering device to carry out the motion of short route when automated inspection, and wherein, liquid-transfering device is arranged in shifting and mixing the liquid in each cell body.

In order to ensure the light transmittance of the material, the first container 11 may be made of transparent PP (Polypropylene). In other embodiments, forebay 101 may also be provided with a transmission detection window (not shown) for cooperating with optical detection, and the transmission and smoothness of the transmission detection window may be the same as forebay 101 or higher than other portions of forebay 101.

Further, the second detection cell site includes a plurality of placement holes 123, and the placement holes 123 are used for placing an optical detection cup assembly (not shown) for optical detection. Wherein the placement holes 123 may include first and second placement holes 121 and 122 that are spaced apart. The optical detection cup assembly comprises an optical measurement cup (not shown) and a reagent cup (not shown), the optical measurement cup can be used for detecting specific protein, the material of the optical measurement cup can be transparent PC (Polycarbonate), and the reagent cup is used for storing reagents. The first placing hole 121 may be used to place an optical measuring cup, and the second placing hole 122 may be used to place a detecting cup.

Here, the first placing hole 121 and the second placing hole 122 are different in shape, for example, the first placing hole 121 may be provided in a circular shape, and the second placing hole 122 may be provided in a rectangular shape for distinction.

Optionally, the second detecting cell sites may be used to place two or more sets of optical detecting cup assemblies, that is, the number of the first placing holes 121 and the second placing holes 122 may be two or more sets for detecting different items.

The kit body 10 of the kit of the above embodiment can be detachably connected, and the assembling and disassembling process is simple and convenient to transport.

In a fourth embodiment, the present application further provides a kit, please refer to fig. 10, fig. 10 is a schematic structural diagram of another embodiment of the kit provided in the present application, and the kit of the present embodiment includes a kit body 10 and at least two pipette heads 60. Wherein, at least two pipette head placing pools are arranged on the box body 10 for respectively placing the at least two pipette heads 60. Wherein the pipette tip 60 is adapted to fit on a pipette for performing pipetting operations.

Each pipette head 60 has a volume such that a sample liquid or a reagent liquid is retained in the pipette head 60 during pipetting, and at least two pipette heads 60 have different volumes in this embodiment. For example, the kit may be provided with two pipette heads 60, and the volumes of the two pipette heads 60 are different. In the actual process, pipette tips 60 with different volumes can be used for different sample liquids or reagent liquids, for example, when 10 μ L of liquid needs to be pipetted, a pipette tip 60 with a volume of 10 μ L to 20 μ L can be selected to ensure pipetting accuracy. By the mode, the sample adding precision can be improved, so that the accuracy of the detection result of the sample is improved.

Further, as shown in fig. 10, the pipette head 60 includes a tube body 61 and a fixing portion 62, the fixing portion 62 is fixed to the periphery of the tube body 61, the tube body 61 is formed with a pipette port 63 for sucking/discharging liquid, the fixing portion 62 is located on a side of the tube body 61 away from the pipette port 63, and the fixing portion 62 is used for fixing the tube body 61 in the pipette head placing tank 113. The fixing portions 62 of the pipette tips 60 having different volumes are different in shape so as to distinguish the pipette tips 60 by the shape of the fixing portions 62, thereby preventing the pipette tips 60 from being selected by mistake. In other embodiments, the shape of the securing portion 62 of the pipette head 60 may be the same for different volumes to facilitate production of the pipette head 60.

The inside diameters of the pipette tip placement wells 113 on the cassette 10 may be the same or different. For example, for ease of differentiation, at least two pipette head placement wells 113 have different inner diameters for respectively placing different pipette heads 60. Specifically, a pipette head 60 having a large volume may be inserted into the pipette head placing cell 113 having a large inner diameter, and a pipette head 60 having a small volume may be inserted into the pipette head placing cell 113 having a small inner diameter.

In order to allow the pipette head 60 to accommodate pipette head placement wells 113 of different inner diameters, the fixing portion 62 of the pipette head 60 may be provided in a stepped shape. Specifically, the fixing portion 62 of the pipette head 60 includes at least two steps, the at least two steps are parallel and spaced apart toward a side away from the liquid suction port 63, and the outer diameters of the at least two steps gradually increase toward a direction away from the liquid suction port 63. Through setting the fixed part 62 to the step of equidimension not, make the suction head 60 can adapt to the suction head of different internal diameters and place pond 113, this kind of mode can make the cartridge of suction head 60 not receive the restriction of the internal diameter that pond 113 was placed to the suction head to, make things convenient for getting of suction head 60 to put.

When the pipette head 60 is placed in the pipette head placement chamber 113, the tube 61 is inserted into the pipette head placement chamber 113, and the pipette head 60 can be supported on the surface of the cassette 10 by the fixing part 62.

Further, a sunken platform 1131 may be disposed at an opening of the pipette head placing tank 113, and when the pipette head 60 is placed in the pipette head placing tank 113, the fixing portion 62 may be supported on the sunken platform 1131 to support the pipette head 60 through the sunken platform 1131.

Optionally, the lengths and/or calibers of the pipettes 60 of different volumes may also be different to facilitate distinguishing between different pipettes 60. For example, a pipette head 60 having a smaller volume may also have a smaller length/diameter to facilitate distinguishing between different pipette heads 60.

Further, the inner wall of the pipette head 60 may be provided with a hydrophobic coating to prevent the pipette head 60 from hanging liquid and improve the sample adding precision of the pipette head 60.

Further, the pipette head 60 may be provided with a mark, such as an electronic tag, a two-dimensional code or a bar code, to record the relevant parameters of the pipette head 60.

The kit of the embodiment can improve the sample adding precision, thereby improving the accuracy of the sample detection result.

The structures of the reagent cartridges in the first, second, third and fourth embodiments described above may be combined with each other. For example, the structure of the box body 10 in the fourth embodiment may not be integrally arranged, as shown in the embodiments of fig. 6 to 9, the box body 10 may include a first box body 11 and a second box body 12, and the first box body 11 and the second box body 12 are detachably connected, for which reference is specifically made to the drawings and the related text description of the above embodiments, and details are not repeated here.

In a fifth embodiment, the present application further provides a point-of-care testing (POCT) blood cell analyzer, please refer to fig. 11, which is a schematic structural diagram of an embodiment of a pipette tip and a pipette tip in the POCT blood cell analyzer provided in the present application, the POCT blood cell analyzer includes a pipette 70, and an end of the pipette 70 is used to cooperate with the pipette tip 60 of the above embodiment to perform a pipetting operation.

Specifically, the tip of the pipette 70 is provided with a suction head 71, the suction head 71 is used for sleeving the pipette head 60, the pipette head 60 has a certain volume, and when a sample liquid or a reagent liquid is retained in the pipette head 60 during a pipetting operation and does not enter the inside of the pipette 70, the pipette head 60 can discard the used pipette head 60 and reinstall the unused pipette head 60 when the pipetting, moving and spitting operations are completed and the sample liquid or the reagent liquid needs to be replaced, so that a new sample liquid or reagent liquid can be pipetted, and therefore, the pipette 70 is not polluted, and therefore, the pipette 30 does not need to be cleaned after being used every time, a complex cleaning assembly and a cleaning flow are omitted, and the detection efficiency is improved.

Further, the pipette head 71 at the end of the pipette 70 is stepped for accommodating pipette heads 60 of different calibers. In this way, pipette tips 60 of different diameters can be connected by one pipette tip 71, so that the pipetting process is simplified and the material cost is saved.

The embodiment of the present application further provides a point-of-care testing (POCT) blood cell analyzer, which includes the kit of any of the foregoing embodiments and a detection seat matched with the kit, and the POCT blood cell analyzer is used for analyzing a blood sample. For the specific structure of the kit, reference is made to the drawings of the foregoing embodiments and the related text descriptions, which are not repeated herein.

The above description is only an embodiment of the present application, and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes performed by the present application and the contents of the attached drawings, which are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.

Claims (10)

1. A kit, comprising:

at least two pipette heads for aspirating liquids, the at least two pipette heads having different volumes;

the box body is provided with at least two pipette head placing pools for placing the at least two pipette heads.

2. The kit according to claim 1, wherein the at least two pipette heads have different lengths and/or inner diameters, and the at least two pipette head placement wells have different inner diameters for placing the at least two pipette heads, respectively.

3. The kit of claim 2, wherein each pipette head comprises:

a tube body formed with a liquid suction port;

the fixed part, set up in the periphery of body, be used for with the body is fixed in the pond is placed to the pipette head, the fixed part is located the body is kept away from the one end of imbibition mouth, the fixed part includes two at least steps, two at least steps are towards keeping away from one side interval distribution of imbibition mouth, just the external diameter of two at least steps is towards keeping away from the direction crescent of imbibition mouth.

4. The kit of claim 1, wherein the interior wall of the pipette head is provided with a hydrophobic coating.

5. The kit according to claim 1, wherein the pipette head comprises a tube body and a fixing part fixed to the outer periphery of the tube body for fixing the tube body to the pipette head placement chamber, wherein the fixing parts of the at least two pipette heads are different in shape.

6. The kit of claim 1, wherein the cartridge comprises:

the first box body is provided with at least two pipette head placing pools, and is also provided with a first detection pool position used for electrical impedance detection;

the second box body is provided with a second detection pool position, the second detection pool position is used for optical detection, and the first box body is detachably connected with the second box body.

7. The kit according to claim 6, wherein at least one slot is provided on one side of the first box, and a protrusion engaged with the slot is provided on one side of the second box.

8. The kit according to claim 6, wherein a hanger is arranged on one side of the first box body, and at least two ends of the second box body are hung on the hanger.

9. A POCT cytometer comprising a pipette and the kit of any one of claims 1 to 8, wherein the tip of the pipette is used to cooperate with a pipette tip in the kit to perform pipetting operations.

10. The POCT blood cell analyzer of claim 9, wherein a tip is disposed at an end of the pipette for engaging with the pipette head, wherein the tip is stepped to accommodate pipette heads with different calibers.

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