CN115678544B - Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof - Google Patents
Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof Download PDFInfo
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- CN115678544B CN115678544B CN202211310305.3A CN202211310305A CN115678544B CN 115678544 B CN115678544 B CN 115678544B CN 202211310305 A CN202211310305 A CN 202211310305A CN 115678544 B CN115678544 B CN 115678544B
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 239000010931 gold Substances 0.000 title claims abstract description 32
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 32
- 239000003446 ligand Substances 0.000 title claims abstract description 15
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 238000001308 synthesis method Methods 0.000 title claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 14
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- 229930003451 Vitamin B1 Natural products 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 8
- 229960003495 thiamine Drugs 0.000 claims abstract description 8
- 239000011691 vitamin B1 Substances 0.000 claims abstract description 8
- 235000010374 vitamin B1 Nutrition 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 6
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims abstract description 5
- 239000003513 alkali Substances 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims abstract 3
- 229930003270 Vitamin B Natural products 0.000 claims description 3
- 235000019156 vitamin B Nutrition 0.000 claims description 3
- 239000011720 vitamin B Substances 0.000 claims description 3
- 238000006862 quantum yield reaction Methods 0.000 abstract description 7
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 101150113720 aunc gene Proteins 0.000 description 5
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 150000007529 inorganic bases Chemical class 0.000 description 2
- 150000007530 organic bases Chemical class 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- BSKHPKMHTQYZBB-UHFFFAOYSA-N 2-methylpyridine Chemical compound CC1=CC=CC=N1 BSKHPKMHTQYZBB-UHFFFAOYSA-N 0.000 description 1
- WUPHOULIZUERAE-UHFFFAOYSA-N 3-(oxolan-2-yl)propanoic acid Chemical compound OC(=O)CCC1CCCO1 WUPHOULIZUERAE-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012984 biological imaging Methods 0.000 description 1
- 229910052980 cadmium sulfide Inorganic materials 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229910052981 lead sulfide Inorganic materials 0.000 description 1
- 229940056932 lead sulfide Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Abstract
The invention discloses a gold nanocluster with vitamin B1 as a ligand and a synthesis method thereof, and relates to the technical field of chemical synthesis. The invention relates to a method for preparing chloroauric acid and vitamin B 1 Stirring and mixing uniformly in water to obtain a mixed solution; adding an alkali solution into the mixed solution, adjusting the pH to be alkaline (the pH is more than or equal to 7.5), and obtaining the gold nanocluster taking vitamin B1 as a ligand only through stirring reaction at room temperature. Meanwhile, pyridine is added in the reaction process, so that the fluorescence quantum yield of the pyridine can be greatly improved. The synthesis method is simple and shortens the synthesis time of the gold nanoclusters. The invention uses vitamin B 1 Gold nanoclusters, which are ligands, have higher quantum yields and can be used to construct various types of fluorescence sensors.
Description
Technical Field
The invention belongs to the technical field of gold nanoclusters, and in particular relates to a vitamin B-based nano-particle 1 Gold nanoclusters as ligands and methods of synthesizing the same.
Background
Gold nanoclusters (AuNCs) are a special type of nano fluorescent material consisting of several to hundreds of gold atoms, whose size is equal to the fermi wavelength close to electrons, which gives gold nanoclusters with molecular-like characteristics and discrete energy levels, exhibiting excellent optical, chemical and electronic characteristics. Fluorescence (FL) is one of the most important characteristics of gold nanoclusters, and compared with organic dyes and semiconductor quantum dots (cadmium sulfide, lead sulfide and the like), the fluorescent dye has the advantages of low toxicity, good water solubility, good biocompatibility, good light stability and the like, so that the fluorescent dye has wide application prospects in the fields of biological imaging, sensing, environment detection and the like.
At present, the gold nanocluster has the defects of low quantum yield (QY, usually < 10%), complex synthesis process, long preparation period and the like, which severely limits the practical application of the gold nanocluster. Therefore, developing a simple, fast synthetic and high quantum yield method for gold nanoclusters is a hotspot of current research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a rapid gold nanocluster synthesis method with high quantum yield, which is realized by adopting the following technical scheme:
vitamin B 1 A method for synthesizing gold nanoclusters that are ligands, comprising the steps of:
(1) Chloroauric acid and vitamin B 1 Stirring and mixing uniformly in water to obtain a mixed solution;
(2) Adding an alkali solution into the mixed solution, adjusting the pH to be more than or equal to 7.5, and stirring for reaction to obtain the gold nanocluster with vitamin B1 as a ligand.
Preferably, chloroauric acid and vitamin B in step (1) 1 The molar ratio of (2) is 0.4-1: 1.
preferably, the alkaline solution in step (2) comprises an inorganic or organic base.
More preferably, the inorganic base in step (2) is sodium hydroxide, potassium hydroxide, sodium carbonate or ammonia water.
More preferably, the organic base in step (2) is pyridine and its derivatives, or an organic amine.
Preferably, the stirring temperature in step (1) and step (2) is room temperature or higher and the stirring time is 2min or higher.
Another object of the present invention is to provide a method for producing vitamin B 1 Gold nanoclusters as ligands, which are prepared from vitamin B 1( VB 1 ) As a reducing and stabilizing agent, gold nanoclusters with fluorescence characteristics are rapidly obtained after the pH value of the solution is adjusted at room temperature, and the gold nanoclusters have higher quantum yield.
Compared with the prior art, the invention has the following beneficial effects:
the synthesis method is simple and mild, and shortens the synthesis time of the gold nanoclusters. At the same time, the invention uses vitamin B 1 Gold nanoclusters, which are ligands, have higher quantum yields and can be used to construct various types of fluorescence sensors.
Drawings
FIG. 1 is a synthetic route diagram of the present invention;
FIG. 2 shows AuNCs@VB synthesized in example 1 1 A TEM image of (a);
FIG. 3 is a fluorescence spectrum of the gold nanoclusters synthesized in example 1;
FIG. 4 is a fluorescence spectrum of gold nanoclusters synthesized in example 2;
FIG. 5 is a fluorescence spectrum of the gold nanoclusters synthesized in example 3.
Detailed Description
The following detailed description of the present invention provides for a more complete understanding of the objects, features and advantages of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
Vitamin B 1 Gold nanoclusters as ligands are prepared as follows:
a. mixing chloroauric acid aqueous solution (0.20 mL,10 mM) and vitamin B1 aqueous solution (3.80 mL,0.5 mM), and stirring at room temperature and pressure for 5min to obtain a uniform mixture;
b. 200. Mu.L of 1M NaOH aqueous solution is added to the mixed solution at a time, and the pH is adjusted to 10;
c. and (3) rapidly stirring and reacting for 5min under the conditions of normal temperature and normal pressure. Obtaining vitamin B 1 Gold nanoclusters protected for ligand (AuNCs@VB) 1 ) Preserving at 4deg.C.
Example 2
a. Aqueous chloroauric acid (0.20 mL,10 mM) and vitamin B1 ethanol (3.80 mL,0.5 mM) were mixed and stirred at room temperature and pressure for 5min to mix them uniformly.
b. To the above mixed solution, 70. Mu.L of pyridine (purity: 99.0%) was added at a time;
c. rapidly stirring and reacting for 5min under normal temperature and normal pressure to obtain fluorescence enhanced vitamin B1 protected gold nanocluster (AuNCs@VB) 1 Py) and stored at 4 ℃.
Example 3
a. Aqueous chloroauric acid (2 mL,1 mM) and vitamin B1 ethanol (2 mL,1 mM) were mixed, and stirred at room temperature and pressure for 5min to mix them uniformly.
b. To the above mixed solution, 85. Mu.L of picoline (10M) was added at a time;
c. rapidly stirring and reacting for 5min under normal temperature and normal pressure to obtain fluorescence enhanced vitamin B 1 Protected gold nanoclusters (AuNCs@VB) 1 4M-Py) and stored at 4 ℃.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (3)
1. Vitamin B 1 The synthesis method of the gold nanocluster serving as the ligand is characterized by comprising the following steps of:
(1) Chloroauric acid and vitamin B 1 Stirring and mixing uniformly in water to obtain a mixed solution;
(2) Adding an alkali solution into the mixed solution, adjusting the pH to be more than or equal to 7.5, and stirring for reaction to obtain gold nanoclusters taking vitamin B1 as a ligand;
chloroauric acid and vitamin B in step (1) 1 The molar ratio of (2) is 0.4-1: 1, a step of;
the alkali solution is pyridine and its derivative.
2. The synthesis method according to claim 1, wherein the stirring temperature in step (1) and step (2) is room temperature or higher and the stirring time is not less than 2min.
3. Vitamin B 1 Gold nanoclusters as ligands, characterized in that they are prepared by a method according to any one of claims 1 to 2.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211310305.3A CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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| CN202211310305.3A CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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| CN115678544A CN115678544A (en) | 2023-02-03 |
| CN115678544B true CN115678544B (en) | 2023-11-14 |
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| CN202211310305.3A Active CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
Non-Patent Citations (1)
| Title |
|---|
| 荧光银纳米簇的制备及应用研究;温娜;中国优秀硕士学位论文全文数据库工程科技Ⅰ辑(第12期);第18-28页 * |
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