CN115678544A - Vitamin B 1 Gold nanoclusters as ligands and synthesis method thereof - Google Patents
Vitamin B 1 Gold nanoclusters as ligands and synthesis method thereof Download PDFInfo
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- CN115678544A CN115678544A CN202211310305.3A CN202211310305A CN115678544A CN 115678544 A CN115678544 A CN 115678544A CN 202211310305 A CN202211310305 A CN 202211310305A CN 115678544 A CN115678544 A CN 115678544A
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- vitamin
- gold
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- ligand
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 239000010931 gold Substances 0.000 title claims abstract description 32
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 32
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 239000003446 ligand Substances 0.000 title claims abstract description 14
- 238000001308 synthesis method Methods 0.000 title claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229930003451 Vitamin B1 Natural products 0.000 claims abstract description 8
- 229960003495 thiamine Drugs 0.000 claims abstract description 8
- 239000011691 vitamin B1 Substances 0.000 claims abstract description 8
- 235000010374 vitamin B1 Nutrition 0.000 claims abstract description 8
- 239000003513 alkali Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 150000007529 inorganic bases Chemical class 0.000 claims description 3
- 150000007530 organic bases Chemical class 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 238000006862 quantum yield reaction Methods 0.000 abstract description 6
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 abstract 2
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- BSKHPKMHTQYZBB-UHFFFAOYSA-N 2-methylpyridine Chemical compound CC1=CC=CC=N1 BSKHPKMHTQYZBB-UHFFFAOYSA-N 0.000 description 1
- WUPHOULIZUERAE-UHFFFAOYSA-N 3-(oxolan-2-yl)propanoic acid Chemical compound OC(=O)CCC1CCCO1 WUPHOULIZUERAE-UHFFFAOYSA-N 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- 101150113720 aunc gene Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012984 biological imaging Methods 0.000 description 1
- 229910052980 cadmium sulfide Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229910052981 lead sulfide Inorganic materials 0.000 description 1
- 229940056932 lead sulfide Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
The invention discloses a gold nanocluster taking vitamin B1 as a ligand and a synthesis method thereof, and relates to the technical field of chemical synthesis. The invention relates to a method for preparing chlorauric acid and vitamin B 1 Stirring and mixing evenly in water to obtain a mixed solution; and adding an alkali solution into the mixed solution, adjusting the pH value to be alkaline (the pH value is more than or equal to 7.5), and stirring at room temperature for reaction to obtain the gold nanocluster taking vitamin B1 as a ligand. Meanwhile, the pyridine is added in the reaction process, so that the fluorescence quantum yield of the material can be greatly improved. The synthesis method is simple and shortens the synthesis time of the gold nanoclusters. The invention uses vitamin B 1 The gold nanoclusters as ligands have higher quantaYield, can be used to construct various types of fluorescence sensors.
Description
Technical Field
The invention belongs to the technical field of gold nanoclusters, and particularly relates to a vitamin B 1 A gold nanocluster serving as a ligand and a synthesis method thereof.
Background
Gold nanoclusters (AuNCs) are a special type of nano fluorescent material consisting of several to several hundred gold atoms, the size of which is equal to the fermi wavelength close to electrons, which endows the gold nanoclusters with molecular-like properties and discrete energy levels, exhibiting excellent optical, chemical and electronic properties. Fluorescence (FL) is one of the most important characteristics of gold nanoclusters, and compared with organic dyes and semiconductor quantum dots (cadmium sulfide, lead sulfide and the like), the Fluorescence (FL) has the advantages of low toxicity, good water solubility, good biocompatibility, good light stability and the like, so that the Fluorescence (FL) has wide application prospects in the fields of biological imaging, sensing, environmental detection and the like.
At present, the gold nanocluster has the defects of low quantum yield (QY, generally less than 10%), complex synthesis process, long preparation period and the like, so that the practical application of the gold nanocluster is severely limited. Therefore, developing a method of simply, rapidly synthesizing and high quantum yield gold nanoclusters is a hot spot of current research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a gold nanocluster synthesis method with high quantum yield and high speed, which is realized by adopting the following technical scheme:
vitamin B 1 A method of synthesizing gold nanoclusters as ligands, comprising the steps of:
(1) Mixing chloroauric acid and vitamin B 1 Stirring and mixing evenly in water to obtain a mixed solution;
(2) And adding an alkali solution into the mixed solution, adjusting the pH value to be more than or equal to 7.5, and stirring for reaction to obtain the gold nanocluster taking vitamin B1 as a ligand.
Preferably, in step (1), chloroauric acid and vitamin B 1 In a molar ratio of 0.4 to 1:1.
preferably, the alkali solution in step (2) comprises an inorganic alkali or an organic alkali.
More preferably, the inorganic base in step (2) is sodium hydroxide, potassium hydroxide, sodium carbonate or ammonia water.
More preferably, the organic base in step (2) is pyridine and its derivatives, or organic amine.
Preferably, the stirring temperature in the step (1) and the step (2) is room temperature or above, and the stirring time is 2min or above.
Another object of the present invention is to provide a vitamin B 1 Gold nanoclusters as ligands, which are vitamin B 1( VB 1 ) As a reduction and stabilizer, the gold nanoclusters with fluorescence characteristics are quickly obtained after the pH value of the solution is adjusted at room temperature, and the gold nanoclusters have high quantum yield.
Compared with the prior art, the invention has the following beneficial effects:
the synthesis method is simple and mild, and shortens the synthesis time of the gold nanoclusters. Meanwhile, the invention uses vitamin B 1 The gold nanoclusters serving as ligands have high quantum yield and can be used for constructing various types of fluorescence sensors.
Drawings
FIG. 1 is a synthetic route diagram according to the present invention;
FIG. 2 is AuNCs @ VB synthesized in example 1 1 A TEM image of (B);
FIG. 3 is a fluorescence spectrum of the gold nanoclusters synthesized in example 1;
FIG. 4 is a fluorescence spectrum of the gold nanoclusters synthesized in example 2;
fig. 5 is a fluorescence spectrum of the gold nanoclusters synthesized in example 3.
Detailed Description
The present invention will be described in detail with reference to the following embodiments in order to make the objects, features and advantages thereof more comprehensible. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
Vitamin B 1 The preparation method of the gold nanocluster serving as the ligand comprises the following steps:
a. mixing chloroauric acid aqueous solution (0.20mL, 10mM) and vitamin B1 aqueous solution (3.80mL, 0.5mM), and stirring gently at normal temperature and normal pressure for 5min to mix uniformly;
b. adding 200 μ L of 1M NaOH aqueous solution into the mixed solution at one time, and adjusting pH to 10;
c. and rapidly stirring and reacting for 5min under the conditions of normal temperature and normal pressure. Obtaining vitamin B 1 Gold nanocluster for ligand protection (AuNCs @ VB) 1 ) And storing at 4 ℃.
Example 2
a. An aqueous chloroauric acid solution (0.20mL, 10mM) and an ethanol vitamin B1 solution (3.80mL, 0.5mM) were mixed, and the mixture was stirred gently at normal temperature and pressure for 5 minutes to mix them uniformly.
b. To the above mixed solution was added 70. Mu.L of pyridine (purity 99.0%) at once;
c. rapidly stirring at normal temperature and pressure for 5min to obtain gold nanocluster (AuNCs @ VB) protected by fluorescence enhanced vitamin B1 1 Py), and storing at 4 ℃.
Example 3
a. An aqueous chloroauric acid solution (2mL, 1mM) and an ethanol vitamin B1 solution (2mL, 1mM) were mixed, and the mixture was stirred gently at normal temperature and pressure for 5min to mix well.
b. To the above mixed solution was added 85. Mu.L of picoline (10M) at once;
c. rapidly stirring and reacting for 5min under normal temperature and pressure to obtain fluorescence enhanced vitamin B 1 Protected gold nanocluster (AuNCs @ VB) 1 and/4M-Py) and storing at 4 ℃.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent should be subject to the appended claims.
Claims (7)
1. Vitamin B 1 A method for synthesizing a gold nanocluster as a ligand, comprising the steps of:
(1) Mixing chloroauric acid and vitamin B 1 Stirring and mixing evenly in water to obtain a mixed solution;
(2) And adding an alkali solution into the mixed solution, adjusting the pH value to be more than or equal to 7.5, and stirring for reaction to obtain the gold nanoclusters with vitamin B1 as a ligand.
2. The method of claim 1, wherein in step (1) chloroauric acid and vitamin B 1 In a molar ratio of 0.4 to 1:1.
3. the method of claim 1, wherein the alkali solution of step (2) comprises an inorganic or organic base.
4. The synthesis method according to claim 3, wherein the inorganic base in step (2) is sodium hydroxide, potassium hydroxide, sodium carbonate or ammonia water.
5. The method of claim 3, wherein the organic base in step (2) is pyridine or its derivatives or organic amine.
6. The synthesis method of claim 1, wherein the stirring temperature in the step (1) and the step (2) is room temperature or higher, and the stirring time is not less than 2min.
7. Vitamin B 1 A gold nanocluster as a ligand, characterized by being produced by the method of any one of claims 1 to 6.
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|---|---|---|---|
| CN202211310305.3A CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211310305.3A CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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| Publication Number | Publication Date |
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| CN115678544A true CN115678544A (en) | 2023-02-03 |
| CN115678544B CN115678544B (en) | 2023-11-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211310305.3A Active CN115678544B (en) | 2022-10-25 | 2022-10-25 | Vitamin B 1 Gold nanocluster serving as ligand and synthesis method thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
Non-Patent Citations (1)
| Title |
|---|
| 温娜: "荧光银纳米簇的制备及应用研究", 中国优秀硕士学位论文全文数据库工程科技Ⅰ辑, no. 12, pages 18 - 28 * |
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| CN115678544B (en) | 2023-11-14 |
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