CN115671362A - Alginic acid polysaccharide polymer gel film agent and preparation method and application thereof - Google Patents
Alginic acid polysaccharide polymer gel film agent and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides an alginic acid polysaccharide polymer gel film agent, a preparation method and an application thereof, belonging to the technical field of medical supplies. The alginic acid polysaccharide polymer gel membrane agent provided by the invention forms gel by utilizing the complexing action of sodium alginate, arginine and zinc ions, and differential scanning calorimetry analysis and infrared prove that the sodium alginate, arginine and zinc ions are successfully complexed together to obtain the novel alginic acid polysaccharide polymer gel membrane agent.
Description
Technical Field
The invention relates to the technical field of medical supplies, in particular to an alginic acid polysaccharide polymer gel film agent, a preparation method and application thereof.
Background
The skin is an important barrier and the first line of defense, and is an important immune organ for the human body to resist external injury. Skin trauma caused by external injury or insufficient autoimmunity is a significant challenge to healthcare systems worldwide. Wounds on the skin can lead to the invasion of pathogens, causing inflammation or infection, possibly leading to serious illness, affecting the wound healing process. Wound healing is a dynamic biological process involving cell type, extracellular matrix (ECM) molecules and growth factor events to restore the integrity and functionality of damaged skin. There are four distinct but interrelated overlapping stages of wound healing: hemostasis and inflammation, proliferation, maturation and remodeling.
Wound dressings are used for wound management, which may provide a physical barrier between the wound and the external environment to prevent further injury or infection. At present, wound repair dressings on the market are various in types, but most of the wound repair dressings are complicated in operation steps and the repair effect is not obvious. Therefore, the development of wound dressing with simple preparation method and obvious wound repair effect is urgently needed.
Disclosure of Invention
In view of the above, the invention aims to provide an alginic acid polysaccharide polymer gel membrane agent, and a preparation method and an application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
a preparation method of alginic acid polysaccharide gel film agent comprises the following steps:
s1, dissolving sodium alginate in deionized water, and uniformly stirring to obtain a sodium alginate solution;
s2, sequentially adding arginine and glycerol into the sodium alginate solution obtained in the S1, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
s3, pouring the sodium alginate arginine solution obtained in the S2 into a mold, and drying to obtain a sodium alginate arginine dry film;
s4, dissolving zinc sulfate in deionized water, and uniformly stirring to obtain a zinc sulfate solution;
and S5, quickly pouring the zinc sulfate solution obtained in the step S4 into the sodium alginate arginine dry film obtained in the step S3, cross-linking for a period of time, taking out, washing, and absorbing surface moisture by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
Preferably, the polymerization degree of the sodium alginate in the S1 is 200-400; the mass concentration of the sodium alginate in the sodium alginate solution obtained in the S1 is 0.2 g/L-50 g/L.
Preferably, the mass concentration of arginine in the sodium alginate-arginine mixed solution in S2 is 0.1-20 g/L, and the addition amount of glycerol is 5-30% of the volume of the sodium alginate-arginine mixed solution.
Preferably, the drying temperature is 35-50 ℃, and the drying time is 20-30 h; the thickness of the sodium alginate arginine dry film is 0.2 mm-0.3 mm.
Preferably, the mass concentration of the zinc sulfate in the zinc sulfate solution in S4 is 3 g/L-100 g/L.
Preferably, the pouring amount of the zinc sulfate solution in the S5 is 0.8 to 1.5 times of the volume of the sodium alginate arginine solution; the crosslinking temperature of the crosslinking is 20-25 ℃, and the crosslinking time is 0.5-1.5 h; the washing is performed by using distilled water.
Preferably, the stirring is magnetic stirring at room temperature.
The invention also provides the alginic acid polysaccharide polymer gel membrane prepared by the preparation method of the alginic acid polysaccharide polymer gel membrane.
The invention also provides application of the alginic acid polysaccharide polymer gel membrane in preparing a dressing for healing skin wound surfaces.
The beneficial technical effects are as follows: the invention provides an alginic acid polysaccharide gel membrane agent and a preparation method and application thereof, the invention utilizes the complexation of sodium alginate, arginine and zinc ions to form gel, differential scanning calorimetry analysis and infrared prove that the sodium alginate, arginine and zinc ions are successfully complexed together to obtain the novel alginic acid polysaccharide gel membrane agent, experiments prove that the polymer material has multiple effects of isolating bacteria, keeping the wound surface moist, resisting oxidation, stopping bleeding, promoting wound surface healing and the like, and the preparation method provided by the invention has the advantages of cheap and easily obtained main raw materials, mild synthesis conditions, simple production route, simple process flow, strong operability and easier control of production quality, and has higher safety and clinical use value compared with the existing similar products.
Drawings
FIG. 1 is a DSC chart of differential scanning calorimetry, in which a represents sodium alginate, b represents arginine, c represents zinc sulfate, d represents a physical mixture of sodium alginate, arginine and zinc sulfate, and e represents an alginic acid polysaccharide polymer gel film agent;
FIG. 2 is a graph showing the swelling ratio of alginic acid polysaccharide gel film agent;
FIG. 3 is an antioxidant map of in vitro antioxidant assay, in which SA represents sodium alginate, arg represents arginine, SA-Arg-Zn 2+ Representing alginic acid polysaccharide polymer gel film agent, and Vc representing ascorbic acid;
FIG. 4 shows the results of in vitro antibacterial tests, wherein a represents blank control, b represents alginic acid polysaccharide polymer gel film agent, c represents sodium alginate, d represents sodium alginate arginine dry film, the right side is the results of in vitro antibacterial tests of Staphylococcus aureus, and the left side is the results of in vitro antibacterial tests of Escherichia coli.
FIG. 5 shows the results of an in vitro hemolytic test; wherein SA represents sodium alginate, SA-Arg represents sodium alginate-arginine, SA-Arg-Zn 2+ Represents alginic acid polysaccharide gel film agent; positiveconol represents a positive control;
figure 6 shows the results of the wound healing test.
Detailed Description
The invention provides a preparation method of alginic acid polysaccharide polymer gel film agent, which comprises the following steps:
s1, dissolving sodium alginate in deionized water, and uniformly stirring to obtain a sodium alginate solution;
s2, sequentially adding arginine and glycerol into the sodium alginate solution obtained in the S1, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
s3, pouring the sodium alginate arginine solution obtained in the step S2 into a mold, and drying to obtain a sodium alginate arginine dry film;
s4, dissolving zinc sulfate in deionized water, and uniformly stirring to obtain a zinc sulfate solution;
and S5, quickly pouring the zinc sulfate solution obtained in the step S4 into the sodium alginate arginine dry film obtained in the step S3, cross-linking for a period of time, taking out, washing, and absorbing surface moisture by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
The method comprises the steps of dissolving sodium alginate in deionized water, and uniformly stirring to obtain a sodium alginate solution.
In the present invention, the polymerization degree of sodium alginate is preferably 200 to 400, more preferably 300; the mass concentration of the sodium alginate in the sodium alginate solution is preferably 0.2 g/L-50 g/L, and more preferably 5 g/L-20 g/L.
After the sodium alginate solution is obtained, the invention takes the obtained sodium alginate solution, sequentially adds arginine and glycerol, and uniformly stirs to obtain the sodium alginate-arginine mixed solution.
In the present invention, the arginine is L-arginine (L-Arg) in a powdery form. L-Arg has guanidino with high positive charge, has remarkable antibacterial effect on gram-positive bacteria and gram-negative bacteria, can provide electrons for free radicals through the guanidino group and react with the free radicals to terminate free radical chain reaction, and shows reduction capability and oxidation resistance. Meanwhile, endothelial cells and macrophages at the wound can metabolize L-Arg into NO through Nitric Oxide Synthase (NOS), the generated NO can stabilize hypoxia inducible factor-1 alpha, and the endothelial cells, the macrophages and fibroblasts are stimulated to secrete vascular endothelial growth factors, so that angiogenesis is induced, and the method plays a vital role in physiological and pathological angiogenesis. However, free L-Arg is easily dissolved in the peripheral plasma, rather than being concentrated on the wound surface, which reduces the efficiency of L-Arg and makes it impossible to effectively utilize L-Arg. The invention cross-links L-Arg with zinc ions and sodium alginate, can be concentrated on the part of the wound surface during the treatment of the wound surface, and effectively plays the roles of resisting bacteria and promoting vascular healing of the L-Arg.
In the invention, the mass concentration of arginine in the sodium alginate-arginine mixed solution is preferably 0.1-20 g/L, and more preferably 1-2 g/L.
In the invention, the addition amount of the glycerol is preferably 5-30% of the volume of the sodium alginate-arginine mixed solution, and more preferably 5-15% of the volume of the sodium alginate-arginine mixed solution.
After the sodium alginate arginine mixed solution is obtained, the sodium alginate arginine solution is poured into a mould and dried to obtain the sodium alginate arginine dry film.
In the invention, the mould can be designed into moulds with different sizes and shapes according to the requirements of products; the material of the mould is preferably glass, plastic or 304 stainless steel, the mould needs to be sterilized before the sodium alginate arginine solution is poured into the mould, and the sterilization treatment can be carried out by selecting a conventional sterilization method according to the material of the mould;
in the invention, the drying temperature for drying is preferably 35-50 ℃, more preferably 37 ℃, and the drying time is preferably 20-30 h, more preferably 24h; the thickness of the sodium alginate arginine dry film is preferably 0.2 mm-0.3 mm. The invention adopts drying treatment, compared with the traditional method of forming gel by freeze-drying sodium alginate, the surface is smoother, more uniform and more flexible, and the distribution of sodium alginate and arginine in the obtained dry film of alginic acid arginine is more uniform and firmer.
The invention dissolves zinc sulfate in deionized water, and the zinc sulfate solution is obtained after uniform stirring.
In the invention, the mass concentration of the zinc sulfate in the zinc sulfate solution is preferably 3 g/L-100 g/L, and more preferably 10 g/L-20 g/L.
After a zinc sulfate solution is obtained, the obtained zinc sulfate solution is quickly poured into the obtained sodium alginate arginine dry film, the sodium alginate arginine dry film is taken out after cross-linking for a period of time, and the film is washed, and the surface water is absorbed by filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
In the invention, the pouring amount of the zinc sulfate solution poured into the container quickly is preferably 0.8-1.5 times of the volume of the sodium alginate arginine solution, and more preferably 1 time of the volume of the sodium alginate arginine solution; the crosslinking temperature of the crosslinking is preferably 20-25 ℃, more preferably 25 ℃, and the crosslinking time is preferably 0.5-1.5 h, more preferably 1h; the washing is performed by using distilled water.
In the invention, the crosslinking can combine sodium alginate, arginine and zinc ions together through complexation to obtain the novel alginic acid polysaccharide polymer gel membrane agent, and the polymer material has multiple effects of isolating bacteria, keeping the wound surface moist, resisting oxidation, stopping bleeding, promoting wound surface healing and the like, and has higher safety and clinical use value compared with the existing similar products. In the invention, the chelating state of the alginic acid polysaccharide polymer gel film agent is detected by Differential Scanning Calorimetry (DSC); the properties of the polymer material are verified by a swelling ratio test, an in vitro oxidation resistance test, an in vitro antibacterial test, an in vitro hemolytic test and a wound healing test respectively.
In the invention, the stirring is preferably performed by magnetic stirring at room temperature, the rotation speed of the magnetic stirring is preferably 600rpm, and the stirring time is preferably 10-14 h.
The invention also provides the alginic acid polysaccharide polymer gel membrane prepared by the preparation method of the alginic acid polysaccharide polymer gel membrane.
In the present invention, the preparation method of the alginic acid polysaccharide polymer gel film agent is the same as that described above, and is not described herein again.
The invention also provides application of the alginic acid polysaccharide polymer gel membrane in preparing a dressing for healing skin wound surfaces.
In the invention, the alginic acid polysaccharide polymer gel membrane agent is natural and degradable, can absorb wound effusion, maintain a wound moist environment, resist bacteria and promote wound healing, cannot be adhered to wound tissues, and cannot cause secondary damage to the wound surface even if the polymer material is removed.
The preparation method of the skin wound healing dressing is not particularly limited, and the preparation method of the corresponding dressing is adopted. The invention has no special limitation on the existence form and the dosage of the alginic acid polysaccharide polymer gel film agent in the skin wound dressing, and the alginic acid polysaccharide polymer gel film agent can be prepared by adopting the conventional existence form and the conventional dosage in the dressing.
For better understanding of the present invention, the following examples are given for further illustration of the present invention, but the present invention is not limited to the following examples.
In the present invention, all reagents used in the following examples and test examples are commercially available reagents and are pure in chromatography or analytical grade unless otherwise specified.
Example 1
(1) Dissolving 0.1g of sodium alginate with the polymerization degree of 300 in 20mL of deionized water, and magnetically stirring at 600rpm at room temperature for 12 hours to obtain a sodium alginate solution;
(2) Dissolving 0.02g of arginine powder and 1mL of glycerol in the sodium alginate solution, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
(3) Pouring the mixed solution of sodium alginate and arginine into a glass mold with the thickness of 9cm multiplied by 9cm, drying at 37 ℃ for 24h to obtain a dry film of sodium alginate and arginine, wherein the thickness of the dry film of sodium alginate and arginine is 0.3mm.
(4) Weighing 0.4g of zinc sulfate, dissolving the zinc sulfate in 40mL of deionized water, and magnetically stirring at room temperature to obtain a zinc sulfate solution;
(5) And (2) quickly pouring a zinc sulfate solution with the volume 1.0 time of that of the sodium alginate arginine solution into the sodium alginate arginine dry film, crosslinking for 1h at 25 ℃, taking out, washing with distilled water, and absorbing water on the surface of the gel film by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
Example 2
(1) Dissolving 0.004g of sodium alginate with the polymerization degree of 200 in 20mL of deionized water, and magnetically stirring at 600rpm at room temperature for 10 hours to obtain a sodium alginate solution;
(2) Dissolving 0.002g of arginine powder and 1mL of glycerol in the sodium alginate solution, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
(3) Pouring the mixed solution of sodium alginate and arginine into a plastic mould with the thickness of 9cm multiplied by 9cm, drying at 50 ℃ for 20h to obtain a dry film of sodium alginate and arginine, wherein the thickness of the dry film of sodium alginate and arginine is 0.2mm.
(4) Weighing 0.12g of zinc sulfate, dissolving the zinc sulfate in 40mL of deionized water, and magnetically stirring at room temperature to obtain a zinc sulfate solution;
(5) And (3) quickly pouring zinc sulfate solution with the volume 0.8 times that of the sodium alginate arginine solution into the sodium alginate arginine dry film, crosslinking at 25 ℃ for 0.5h, taking out, washing with distilled water, and absorbing water on the surface of the gel film by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
Example 3
(1) Taking 0.4g of sodium alginate with the polymerization degree of 400, dissolving the sodium alginate in 20mL of deionized water, and magnetically stirring the solution at the room temperature at 600rpm for 13 hours to obtain a sodium alginate solution;
(2) Dissolving 0.04g of arginine powder and 3mL of glycerol in the sodium alginate solution, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
(3) Pouring the mixed solution of sodium alginate and arginine into a glass mold with the thickness of 9cm multiplied by 9cm, drying at the temperature of 35 ℃ for 30h to obtain a dry film of sodium alginate and arginine, wherein the thickness of the dry film of sodium alginate and arginine is 0.3mm.
(4) Weighing 0.2g of zinc sulfate, dissolving the zinc sulfate in 40mL of deionized water, and magnetically stirring at room temperature to obtain a zinc sulfate solution;
(5) And (2) quickly pouring zinc sulfate solution with the volume 1.5 times that of the sodium alginate arginine solution into the sodium alginate arginine dry film, crosslinking at 20 ℃ for 1.5h, taking out, washing with distilled water, and absorbing water on the surface of the gel film by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
Example 4
(1) Dissolving 1g of sodium alginate with the polymerization degree of 300 in 20mL of deionized water, and magnetically stirring at 600rpm at room temperature for 14h to obtain a sodium alginate solution;
(2) Dissolving 0.4g of arginine powder and 6mL of glycerol in the sodium alginate solution, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
(3) Pouring the mixed solution of sodium alginate and arginine into a 304 stainless steel mold with the size of 9cm multiplied by 9cm, drying at 40 ℃ for 24h to obtain a sodium alginate arginine dry film, wherein the thickness of the sodium alginate arginine dry film is 0.3mm.
(4) Weighing 4g of zinc sulfate, dissolving the zinc sulfate in 40mL of deionized water, and magnetically stirring at room temperature to obtain a zinc sulfate solution;
(5) And (2) quickly pouring zinc sulfate solution with the volume 1.3 times that of the sodium alginate arginine solution into the sodium alginate arginine dry film, crosslinking at 20 ℃ for 1.5h, taking out, washing with distilled water, and absorbing water on the surface of the gel film by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
Test example 1 Differential Scanning Calorimetry (DSC) measurement
Respectively taking a proper amount of physically mixed samples of sodium alginate, arginine, zinc sulfate, sodium alginate, arginine and zinc sulfate and the alginic acid polysaccharide polymer gel film agent obtained in the example 1, carrying out DSC detection, and scanning at 30-400 ℃. DSC spectra of the physical mixed samples of sodium alginate, arginine, zinc sulfate, sodium alginate, arginine and zinc sulfate and the alginic acid polysaccharide polymer gel film agent prepared in example 1 were determined. The detection result is shown in figure 1, and as can be seen from figure 1, the alginic acid polysaccharide polymer gel membrane agent does not have an absorption peak specific to the raw material, which indicates that sodium alginate, arginine and zinc ions have been successfully chelated, and the novel sodium alginate medical polymer material is obtained.
Test example 2 swelling Rate test
The alginic acid polysaccharide polymer gel film agent (size: 2 cm. Times.2 cm) prepared in example 1 was dried at 37 ℃ to a constant weight, immersed in 10ml PBS buffer (pH = 7.4), and the swollen samples were taken out at 5min, 15min, 25min, 35min, and 45min after immersion, respectively, and the excess water on the surface was blotted with filter paper and weighed. The swelling ratio (S) of the sample was calculated as follows:
S％＝W s -W 0 /W 0 ×100％
wherein, W 0 Represents the weight of the alginic acid polysaccharide gel film agent dried to constant weight, W s The weight of the alginic acid polysaccharide gel film agent after swelling is shown. The results of the swelling ratio test are shown in FIG. 2, consisting ofAs can be seen from FIG. 2, the alginic acid polysaccharide polymer gel film agent can reach swelling balance within about 40min, and the swelling rate can reach about 2000%, which indicates that the prepared alginic acid polysaccharide polymer gel film agent has remarkable capability of absorbing seepage.
Test example 3 in vitro antioxidant test
Drying the alginic acid polysaccharide polymer gel membrane prepared in example 1 at 37 ℃ to constant weight, crushing, preparing solutions with the mass concentrations of the alginic acid polysaccharide polymer gel membrane of 10mg/mL, 20mg/mL, 30mg/mL and 40mg/mL by using PBS buffer (pH = 7.4), and shaking for 24h;
preparing sodium alginate into solutions with the mass concentrations of 5.3mg/mL, 10.6mg/mL, 15.9mg/mL and 21.2mg/mL respectively by using PBS buffer (pH = 7.4); preparing arginine into solutions with arginine mass concentrations of 1.05mg/mL, 2.1mg/mL, 3.16mg/mL and 4.21mg/mL respectively by using PBS buffer (pH = 7.4); ascorbic acid was prepared into a solution with a mass concentration of ascorbic acid of 10mg/mL, 20mg/mL, 30mg/mL, 40mg/mL using a PBS buffer (pH = 7.4).
2mL of each of the alginic acid polysaccharide polymer gel membrane, sodium alginate, arginine and ascorbic acid solutions with different concentrations, and 2mL of each of PBS buffer solution (pH = 7.4) were incubated with 2mL of a 0.1mM DPPH solution at a molar concentration of 30min, and absorbance was measured at a wavelength of 517nm, and the absorbance was measured in parallel for three times.
The DPPH radical scavenging rate of the above substances with different concentrations is calculated by the following formula:
DPPH inhibition ％＝1-(A÷A 0 )×100％
wherein A is the absorbance of the sample and DPPH 0 Absorbance of solvent versus DPPH. As shown in FIG. 3, it can be seen from FIG. 3 that the alginate polysaccharide gel film has a higher radical scavenging ability than sodium alginate alone.
Test example 4 in vitro antibacterial test
Preparing a culture medium: preparing an LB solid culture medium and an LB liquid culture medium according to a conventional method;
preparation of golden grape ballsBacterial suspension: inoculating Staphylococcus aureus to LB liquid culture medium, and culturing at 37 deg.C until the concentration of Staphylococcus aureus is 1.0 × 10 8 CFU/mL or so to obtain staphylococcus aureus bacterial suspension;
putting 100 mu L of the staphylococcus aureus suspension on an LB solid culture medium, and uniformly coating the staphylococcus aureus suspension by using a sterile cotton swab;
and (3) beating the sterile filter paper into a filter paper disc with the diameter of 15mm by using a puncher, respectively placing the sterile filter paper disc, the alginic acid polysaccharide gel membrane agent, the sodium alginate and the sodium alginate-arginine on the solid culture medium coated with the staphylococcus aureus bacterial suspension, wherein the alginic acid polysaccharide gel membrane agent, the sodium alginate and the sodium alginate-arginine are all round with the diameter of 15mm and marked by using a sign pen, culturing for 24 hours at 37 ℃, and observing and recording results. Three replicates were performed.
Coli was subjected to in vitro bacteriostatic tests in the same manner as described above.
The test result is shown in fig. 4, and it can be seen from fig. 4 that the inhibition zone of the alginic acid polysaccharide polymer gel film agent is the largest, and the diameters of the inhibition zones for staphylococcus aureus and escherichia coli can reach more than 20mm, which indicates that the alginic acid polysaccharide polymer gel film agent has significant antibacterial property.
Test example 5 in vitro hemolytic test
Mixing 10mL of fresh rat blood with 1mL of heparin sodium solution with the mass concentration of 1g/L to obtain anticoagulated whole blood;
the anticoagulated whole blood was taken, and mixed with a sterile PBS buffer (pH = 7.4) at a volume ratio of 1:4, centrifuging at 2500rpm for 15min, removing supernatant to obtain red blood cells, and mixing the red blood cells with sterile PBS buffer solution in a volume ratio of 1:9 to form a red blood cell suspension;
mixing 10mg of the alginic acid polysaccharide polymer gel film agent obtained in example 1 with 10mL of the erythrocyte suspension uniformly to obtain an experimental group sample;
taking the red blood cells, and mixing the red blood cells with sterile deionized water according to a volume ratio of 1:9 as a positive control sample;
taking the red blood cell suspension as a negative control sample, taking 1mL of each of the negative control sample, the positive control sample and the experimental group sample, incubating at 37 ℃ for 1h, centrifuging at 2500rpm for 15min, respectively taking supernate, and measuring absorbance at a wavelength of 540 nm; negative control samples, positive control samples and experimental group samples were each run in triplicate.
The above-mentioned hemolytic test was performed on sodium alginate and the sodium alginate-arginine dry film obtained in step 2 of example 1 in the same manner.
The hemolysis rate is calculated as follows:
wherein, OD test Is the absorbance, OD, of the test solution neg Absorbance, OD, of negative control pos The absorbance of the positive control group is obtained. The result of the hemolytic test is shown in fig. 5, and it can be seen from fig. 5 that the hemolytic rates of the alginic acid polysaccharide gel membrane, the sodium alginate and the sodium alginate-arginine are all less than 1.9%, which meets the requirement that the hemolytic rate is less than 5% in GB/T14233.2-2005. Therefore, the alginic acid polysaccharide gel membrane does not cause hemolysis.
Test example 6 wound healing test
Taking 12 Wistar rats with the weight of about 200g, randomly dividing the Wistar rats into a blank group, an adhesive bandage group, a sodium alginate group and an experimental group, wherein each group comprises 3 rats, shaving the backs of the rats, and creating a full-thickness circular wound (1.5 cm multiplied by 1.5 cm) by using scissors;
the blank group is directly bandaged with gauze to wrap the wound without providing any dressing and medicine; the woundplast group treats the wound with the woundplast; the sodium alginate group is prepared by taking a sodium alginate solution with the mass concentration of 10g/L as a dressing after freeze drying, and binding a wound by matching with gauze; the experimental group was coated with 2cm × 2cm of the alginic acid polysaccharide polymer gel film prepared in example 1, and wound was bandaged with gauze; after 14d, the rats were observed for wound healing and the results are shown in FIG. 6. As can be seen from fig. 6, the algal polysaccharide polymer material can significantly promote wound healing.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A preparation method of alginic acid polysaccharide gel film agent is characterized by comprising the following steps:
s1, dissolving sodium alginate in deionized water, and uniformly stirring to obtain a sodium alginate solution;
s2, sequentially adding arginine and glycerol into the sodium alginate solution obtained in the S1, and uniformly stirring to obtain a sodium alginate-arginine mixed solution;
s3, pouring the sodium alginate arginine solution obtained in the step S2 into a mold, and drying to obtain a sodium alginate arginine dry film;
s4, dissolving zinc sulfate in deionized water, and uniformly stirring to obtain a zinc sulfate solution;
and S5, quickly pouring the zinc sulfate solution obtained in the step S4 into the sodium alginate arginine dry film obtained in the step S3, cross-linking for a period of time, taking out, washing, and absorbing surface moisture by using filter paper to obtain the alginic acid polysaccharide polymer gel film agent.
2. The method for preparing the alginic acid polysaccharide gel film agent as claimed in claim 1, wherein the polymerization degree of the sodium alginate in S1 is 200-400; the mass concentration of the sodium alginate in the sodium alginate solution obtained in the S1 is 0.2 g/L-50 g/L.
3. The preparation method of the alginic acid polysaccharide polymer gel membrane agent as claimed in claim 1, wherein the mass concentration of arginine in the sodium alginate-arginine mixed solution in S2 is 0.1-20 g/L, and the addition amount of glycerol is 5-30% of the volume of the sodium alginate-arginine mixed solution.
4. The method for preparing the alginic acid polysaccharide gel film agent as claimed in claim 1, wherein the drying temperature in S3 is 35-50 ℃, and the drying time is 20-30 h; the thickness of the sodium alginate arginine dry film is 0.2 mm-0.3 mm.
5. The preparation method of the alginic acid polysaccharide polymer gel membrane agent of claim 1, wherein the mass concentration of zinc sulfate in the zinc sulfate solution in S4 is 3 g/L-100 g/L.
6. The method for preparing the alginic acid polysaccharide polymer gel membrane as claimed in claim 1, wherein the pouring amount of the zinc sulfate solution in S5 is 0.8-1.5 times of the volume of the sodium alginate arginine solution; the crosslinking temperature of the crosslinking is 20-25 ℃, and the crosslinking time is 0.5-1.5 h; the washing is performed by using distilled water.
7. The method for preparing the alginic acid polysaccharide gel membrane agent according to claim 1, wherein the stirring is magnetic stirring at room temperature.
8. An alginic acid polysaccharide polymer gel film agent prepared by the preparation method of the alginic acid polysaccharide polymer gel film agent of any one of claims 1 to 7.
9. The use of the alginic acid polysaccharide gel membrane of claim 8 in the preparation of a dressing for healing a wound surface on skin.
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