CN115650776A - Composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer - Google Patents
Composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 26
- 238000009264 composting Methods 0.000 title claims abstract description 25
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 69
- 230000004151 fermentation Effects 0.000 claims abstract description 67
- 239000002245 particle Substances 0.000 claims abstract description 41
- 239000002893 slag Substances 0.000 claims abstract description 39
- 239000002002 slurry Substances 0.000 claims abstract description 34
- 239000000463 material Substances 0.000 claims abstract description 30
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- 238000002156 mixing Methods 0.000 claims abstract description 26
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- 230000007480 spreading Effects 0.000 claims abstract description 19
- 238000003892 spreading Methods 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims description 40
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- 230000001737 promoting effect Effects 0.000 claims description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 7
- 230000029087 digestion Effects 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 claims 1
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- 230000000052 comparative effect Effects 0.000 description 7
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- 241000282414 Homo sapiens Species 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
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- 230000001276 controlling effect Effects 0.000 description 3
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- 101100367123 Caenorhabditis elegans sul-1 gene Proteins 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps: s1, a step: mixing biogas residue, biogas slurry and the corrosion-promoting mature residue material, drying, and crushing to obtain mixed residue material particles; and S2, a step: adding a zymophyte solution into the mixed slag charge particles, and performing first-stage fermentation to obtain semi-fermented mixed particles; and S3, a step: adding biogas slurry into the semi-fermented mixed particles, fermenting, aging and spreading and drying in the air in the second stage. Before fermentation, the corrosion-promoting mature slag materials are respectively mixed with the biogas residues and the biogas slurry, and then the bacteria liquid is added for fermentation, so that the fermentation process can be effectively promoted, and complete fermentation is facilitated. And adding biogas slurry again in the fermentation process of the mixed slag particles, continuing fermentation, and aging and spreading and drying in the air, so that the abundance of antibiotic resistance genes in the obtained biogas residue organic fertilizer can be effectively reduced, and the harmlessness of biogas residues and the recycling of biogas slurry are realized.
Description
Technical Field
The invention belongs to the technical field of biogas residue fertilizers, and particularly relates to a composting method for reducing antibiotic resistance genes in a biogas residue organic fertilizer.
Background
In recent years, the large-scale pig raising in China is rapidly developed, the number of traditional rural pig raising households is reduced rapidly, and intensive and specialized pig raising enterprises are increased year by year. According to estimation, the annual marketing amount of the large-scale pig farm in China currently accounts for more than 70% of the total marketing amount. Meanwhile, the large-scale pig raising mode also brings new problems of excessive concentration of excrement, large increase of flushing sewage and the like. At present, the common treatment mode for the problems is to fully collect the breeding manure in a large-scale pig farm and then produce biogas through anaerobic fermentation. The biogas residues are residues generated by anaerobic fermentation of culture manure in a biogas digester or a fermentation tank, contain rich organic substances, nutrient elements and enzyme substances, play a role in promoting the growth of crops, can be used for fertilizing soil and improving the physicochemical property of the soil compared with chemical fertilizers, and are high-quality organic fertilizers.
However, the biogas residues contain a large amount of Antibiotic Resistance Genes (ARGs), which cannot be completely eliminated through an anaerobic fermentation process, and a large amount of ARGs still remain. Therefore, the organic fertilizer produced by aerobic composting by using biogas residues also contains a large amount of ARGs, and when the organic fertilizer is applied to soil, the soil environment is damaged, and the human health is possibly damaged after the organic fertilizer enters a food chain. There has been an increasing number of studies to detect the presence of ARGs in food-borne animals, vegetables, drinking water. Enrichment of ARGs in the human body eventually leads to drug resistance of human intestinal flora, resulting in various intestinal diseases, and most of the diseases need to be treated by antibiotics to promote the rapid exchange frequency of ARGs between human beings and the environment, and the consequent emergence of bacterial multi-drug resistance and superbacteria has also attracted attention.
In order to realize the harmless treatment of the biogas residues, reduce the content of antibiotic resistance genes in the biogas residue organic fertilizer and control the propagation risk of the antibiotic resistance genes, the method is a technical problem to be solved urgently in the production of the biogas residue organic fertilizer at present.
Disclosure of Invention
Therefore, the invention aims to solve the problem that the content of the antibiotic resistance gene in the biogas residue organic fertilizer obtained by the composting method in the prior art is high, and further provides the composting method for reducing the antibiotic resistance gene in the biogas residue organic fertilizer.
The invention provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps:
s1, a step: mixing biogas residue, biogas slurry and the decay promoting slag material, drying and crushing to obtain mixed slag material particles;
and S2, a step: adding a zymophyte solution into the mixed slag charge particles, and performing first-stage fermentation to obtain semi-fermented mixed particles;
and S3, a step: adding biogas slurry into the semi-fermented mixed particles, fermenting, aging and spreading and drying in the air in the second stage.
Further, in the step S1, the mass ratio of the biogas residues, the biogas slurry and the decomposed slag promoting materials is as follows: 300-400:400-500:150-250.
Further, the step S1 comprises the steps of mixing biogas residue with the corrosion-promoting slag material to obtain a first mixture, mixing biogas slurry with the corrosion-promoting slag material to obtain a second mixture, mixing the first mixture with the second mixture, drying and crushing to obtain mixed slag material particles.
Further, the mass ratio of the biogas residues to the decomposed slag promoting materials in the first mixture is 300-400:50-100 parts of; the mass ratio of the biogas slurry to the decomposed slag promoting material in the second mixture is 400-500:100-150 parts of; the mass ratio of the decomposition-promoting slag charge in the first mixture to the second mixture is 1:1-3.
Further, the particle size of the crushed mixed slag particles is 0.2cm-0.5cm.
Further, in the step S3, the mass ratio of the semi-fermented mixed slag particles to the biogas slurry is 800-1000:100-150.
Further, the decomposed slag promoting material is at least one of sawdust, mushroom slag and Chinese medicine slag.
In the step S2, the fermentation time in the first stage in the step S2 is 5 to 15 days, preferably 5 to 7 days, and the fermentation time in the second stage is 5 to 10 days, preferably 7 to 10 days.
Further, the pile is turned once every 3-5 days in the fermentation process.
Further, the total aging time is 15-30 days, and the pile is turned once every 8-10 days in the aging process; the moisture content of the fermentation pile obtained after spreading and airing is 30-35%.
The invention also provides the biogas residue organic fertilizer base material prepared by the composting method.
The technical scheme of the invention has the following advantages:
1. the preparation method of the biogas residue organic fertilizer base material provided by the invention comprises the following steps of S1: mixing biogas residue, biogas slurry and the corrosion-promoting mature residue material, drying, and crushing to obtain mixed residue material particles; and S2, a step: adding a zymophyte solution into the mixed slag charge particles to perform first-stage fermentation; and S3, a step: adding biogas slurry into the semi-fermented mixed particles, performing second-stage fermentation, aging, and spreading and drying in the air. Before fermentation, the corrosion-promoting mature slag materials are respectively mixed with biogas residues and biogas slurry, so that the materials are fully and uniformly mixed; then adding a bacterial liquid for fermentation, so that the fermentation process can be effectively promoted; the biogas slurry is added again in the mixed particle fermentation process, then fermentation is continued, and after aging and spreading and drying treatment, the abundance of antibiotic resistance genes in the obtained biogas residue organic fertilizer can be effectively reduced, and the harmless recycling of biogas residue and biogas slurry resource is realized.
2. The preparation method of the biogas residue organic fertilizer base material provided by the invention comprises the following steps of controlling the mass ratio of biogas residue to decomposed promoting slag material in the first mixture to be 300-400:50-100 parts of; the mass ratio of the biogas slurry to the decomposed slag promoting material in the second mixture is 400-500:100-150 parts of; the mass ratio of the decomposition-promoting slag charge in the first mixture to the second mixture is 1:1-3, the water content of the biogas residues can be effectively regulated and controlled, the fermentation speed of the biogas residues is further increased, the complete degree of fermentation of the biogas residues is improved, and resistance genes in the biogas residues can be effectively reduced.
3. According to the preparation method of the biogas residue organic fertilizer base material, the fermentation completeness of biogas residue can be further improved, the metabolic product amount is improved, and the quality of the biogas residue organic fertilizer is improved by optimizing and limiting the fermentation time of the first-stage fermentation and the second-stage fermentation and the pile turning interval time in the fermentation, or limiting the aging time and the pile turning interval time in the aging, and controlling the water content of the pile after spreading and drying.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific embodiments. This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete and will fully convey the concept of the invention to those skilled in the art, and the present invention will only be defined by the appended claims. The biogas residues, biogas slurry, wood chips and mushroom residues are provided by Jiangxi Zhenghe ecological agriculture Limited company, and the physicochemical properties are shown in Table 1.
TABLE 1 physical and chemical Properties of the materials
| Material(s) | pH | Organic carbon g/kg | Total nitrogen g/kg | Total phosphorus g/kg | Total potassium g/kg |
| Biogas residue | 8.14 | 183.5 | 3.2 | 3.8 | 0.9 |
| Biogas slurry | 7.73 | 9.2 | 1.0 | 0.7 | 0.5 |
| Wood chip | 6.84 | 57.4 | 1.3 | 0.9 | 0.7 |
| Mushroom residue | 8.02 | 284.4 | 14.1 | 6.5 | 10.3 |
Example 1
The embodiment provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps:
s1, a step: uniformly mixing 350kg of biogas residues and 50kg of wood chips to obtain a first mixture, uniformly mixing 450kg of biogas slurry and 150kg of wood chips to obtain a second mixture, mixing the first mixture and the second mixture, drying and crushing to obtain mixed slag particles with the average particle size of 0.3 cm;
and S2, a step: adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1;
and S3, a step: spraying 150kg biogas slurry into the semi-fermented mixed particles, performing second-stage fermentation for 10 days, and then aging and spreading for drying in the air to obtain the biogas residue organic fertilizer. The total fermentation time is 15 days, and the pile is turned once every 3 days in the fermentation process; the total time of aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Example 2
The embodiment provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps:
s1, a step: uniformly mixing 400kg of biogas residues and 50kg of mushroom residues to obtain a first mixture, uniformly mixing 450kg of biogas slurry and 100kg of mushroom residues to obtain a second mixture, mixing the first mixture and the second mixture, drying and crushing to obtain mixed residue material particles with the average particle size of 0.3 cm;
and S2, a step: adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1;
and S3, a step: spraying 150kg biogas slurry into the semi-fermented mixed particles, performing second-stage fermentation for 10 days, and then aging and spreading for drying in the air to obtain the biogas residue organic fertilizer. The total fermentation time is 15 days, and the pile is turned once every 3 days in the fermentation process; the total time of aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Example 3
The composting method for reducing the antibiotic resistance genes in the biogas residue organic fertilizer provided by the embodiment comprises the following steps:
s1, a step: uniformly mixing 350kg of biogas residues and 50kg of wood chips to obtain a first mixture, uniformly mixing 450kg of biogas slurry and 150kg of wood chips to obtain a second mixture, mixing the first mixture and the second mixture, drying and crushing to obtain mixed slag particles with the average particle size of 0.3 cm;
and S2, a step: adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1;
and S3, a step: spraying 150kg biogas slurry into the semi-fermented mixed particles, performing second-stage fermentation for 5 days, and then aging and spreading for drying in the air to obtain the biogas residue organic fertilizer. The total fermentation time is 20 days, and the pile is turned once every 3 days in the fermentation process; the total time of aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Example 4
The composting method for reducing the antibiotic resistance genes in the biogas residue organic fertilizer provided by the embodiment comprises the following steps:
s1, a step: uniformly mixing 350kg of biogas residues, 200kg of wood chips and 450kg of biogas slurry, drying and crushing to obtain mixed slag charge particles with the average particle size of 0.3 cm;
and S2, a step: adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1;
and S3, a step: spraying 150kg biogas slurry into the semi-fermented mixed particles, performing second-stage fermentation for 10 days, and then aging and spreading for drying in the air to obtain the biogas residue organic fertilizer. The total fermentation time is 15 days, and the pile is turned once every 3 days in the fermentation process; the total time of aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Comparative example 1
The comparative example provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps:
s1, a step: uniformly mixing 350kg of biogas residues and 50kg of wood chips to obtain a first mixture, uniformly mixing 450kg of biogas slurry and 150kg of wood chips to obtain a second mixture, mixing the first mixture and the second mixture, drying and crushing to obtain mixed slag particles with the average particle size of 0.3 cm;
and S2, a step: and adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1. The fermentation time is 15 days, and the pile is turned once every 3 days in the fermentation process; the total time of the aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Comparative example 2
The comparative example provides a composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer, which comprises the following steps:
s1, a step: uniformly mixing 350kg of biogas residues and 50kg of wood chips to obtain a first mixture, uniformly mixing 450kg of biogas slurry and 150kg of wood chips to obtain a second mixture, mixing the first mixture and the second mixture, drying and crushing to obtain mixed slag material particles with the average particle size of 0.3 cm;
and S2, a step: adding 10kg of a fermentation bacteria solution (the fermentation bacteria solution is a composite bacteria of saccharomycetes, bacillus subtilis and lactobacillus in a mass ratio of 1. The fermentation time is 15 days, and the pile is turned once every 3 days in the fermentation process; the total time of aging is 25 days, and the pile is turned once every 8 days in the aging process; and (3) spreading and airing to obtain a fermentation pile body with the water content of 33wt%, thus obtaining the biogas residue organic fertilizer.
Experimental example 1
The abundance of Antibiotic Resistance Genes (ARGs) in the biogas residue organic fertilizer obtained in each example and comparative example is tested by the following test methods: genome application of biogas residue organic fertilizer
Extracting with SPIN Kit for diseases Kit (MP Biomedicals, CA, USA), and performing the specific method and steps according to Kit instructions. Then use it
The extracted total DNA was purified by DNA Clean-up Kit (MoBio, CA, USA). The concentration and quality of the extracted DNA (OD 260/OD 280) were determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, DE, USA). And (3) characterizing the ARGs in the biogas residue organic fertilizer by adopting a real-time fluorescent quantitative PCR (qPCR) method. qPCR detection was performed on 10 ARGs of class 4, namely tetracycline type ARGs (tetX, tetT, tetM, tetG), sulfonamide type ARGs (sul 2, sul 1), quinolone type ARGs (qnrD, qnrB), macrolide type ARGs (ermB, ermA).
Table 2 shows the abundance of antibiotic resistance genes of biogas residue organic fertilizer prepared in the examples and comparative examples
The results are shown in table 2, compared with comparative examples 1 and 2, the biogas residue organic fertilizer of examples 1 to 4 of the invention can obviously reduce the total abundance of resistance genes of tetracycline antibiotics, sulfonyl antibiotics, quinolone antibiotics and macrolide antibiotics. And compared with mushroom residues, the wood chips serving as the digestion promoting slag have better effect of reducing antibiotic resistance genes in the biogas residue organic fertilizer.
As is clear from comparison between example 1 and example 3, in example 1, the abundance of the antibiotic resistance gene of the biogas residue organic fertilizer can be further reduced by controlling the fermentation time in the first stage and the second stage within the preferable range of the present invention.
As can be seen from comparison of example 1 with example 4, example 1 can further reduce the abundance of antibiotic resistance genes by preparing the mixed slag granules by the preferred method.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A composting method for reducing antibiotic resistance genes in biogas residue organic fertilizer is characterized by comprising the following steps:
s1, a step: mixing biogas residue, biogas slurry and the corrosion-promoting mature residue material, drying, and crushing to obtain mixed residue material particles;
and S2, a step: adding a zymophyte solution into the mixed slag charge particles, and performing first-stage fermentation to obtain semi-fermented mixed particles;
and S3, a step: adding biogas slurry into the semi-fermented mixed particles, fermenting, aging and spreading and drying in the air in the second stage.
2. A composting method as claimed in claim 1, characterised in that the step S1 comprises mixing biogas residue with a decay-promoting slag charge to obtain a first mixture, mixing first biogas slurry with a decay-promoting slag charge to obtain a second mixture, mixing the first mixture with the second mixture, drying and comminuting to obtain mixed slag charge particles.
3. A composting method as claimed in claim 2, characterised in that the mass ratio of biogas residue to the pro-rotting slag material in the first mixture is 300-400:50-100, wherein the mass ratio of the biogas slurry to the decomposition promoting slag charge in the second mixture is 400-500:100-150, wherein the mass ratio of the decomposed slag promoting materials in the first mixture to the second mixture is 1:1-3.
4. A composting method as claimed in any one of the claims 1-3, characterised in that the particle size of the crushed mixed slag particles is between 0.2cm and 0.5cm.
5. A composting method as claimed in any one of claims 1-4, characterised in that in step S3, the mass ratio of semi-fermented mixed slag particles to biogas slurry is 800-1000:100-150.
6. A composting method as claimed in any one of claims 1-5, characterised in that the digestion promoting clinker is at least one of wood chips, mushroom residue and chinese herb residue.
7. A composting method as claimed in any of the claims 1-6, characterised in that in step S2 the fermentation time in the first stage is 5-15 days, preferably 5-7 days, and the fermentation time in the second stage is 5-10 days, preferably 7-10 days.
8. A composting method as claimed in any of the claims 1-7, characterised in that the pile is turned over every 3-5 days during the fermentation.
9. A composting method as claimed in any of the claims 1-8, characterised in that the total time of ageing is 15-30 days, the stack is turned over every 8-10 days during ageing, and the moisture content of the fermented stack obtained after spreading out is 30-35%.
10. A biogas residue organic fertilizer prepared by the composting method as claimed in any one of claims 1 to 9.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104671860A (en) * | 2015-02-25 | 2015-06-03 | 新疆恒丰现代农业科技股份有限公司 | Bioorganic fertilizer using agricultural waste as raw materials and preparation method of bioorganic fertilizer |
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