CN115636979A - Method for enhancing fiber degradation of sweet potato residue - Google Patents
Method for enhancing fiber degradation of sweet potato residue Download PDFInfo
- Publication number
- CN115636979A CN115636979A CN202211226538.5A CN202211226538A CN115636979A CN 115636979 A CN115636979 A CN 115636979A CN 202211226538 A CN202211226538 A CN 202211226538A CN 115636979 A CN115636979 A CN 115636979A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- potato residue
- extrusion
- degradation
- residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000017020 Ipomoea batatas Species 0.000 title claims abstract description 157
- 235000002678 Ipomoea batatas Nutrition 0.000 title claims abstract description 157
- 238000000034 method Methods 0.000 title claims abstract description 37
- 239000000835 fiber Substances 0.000 title claims abstract description 35
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 30
- 230000015556 catabolic process Effects 0.000 title claims abstract description 29
- 230000002708 enhancing effect Effects 0.000 title claims description 15
- 238000001125 extrusion Methods 0.000 claims abstract description 68
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 51
- 235000011187 glycerol Nutrition 0.000 claims abstract description 19
- 230000001007 puffing effect Effects 0.000 claims abstract description 14
- 238000012545 processing Methods 0.000 claims abstract description 7
- 238000005728 strengthening Methods 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 238000001035 drying Methods 0.000 claims description 23
- 238000007873 sieving Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000012805 post-processing Methods 0.000 claims 1
- 239000001913 cellulose Substances 0.000 abstract description 17
- 229920002678 cellulose Polymers 0.000 abstract description 17
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 abstract description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 14
- 239000008103 glucose Substances 0.000 abstract description 14
- 229920002488 Hemicellulose Polymers 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 10
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 abstract description 8
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 abstract description 8
- 238000010438 heat treatment Methods 0.000 abstract description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 3
- 235000019728 animal nutrition Nutrition 0.000 abstract description 2
- 238000009835 boiling Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 46
- 230000000052 comparative effect Effects 0.000 description 16
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 14
- 239000012153 distilled water Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 8
- 229920005610 lignin Polymers 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 238000002441 X-ray diffraction Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 229940059442 hemicellulase Drugs 0.000 description 3
- 108010002430 hemicellulase Proteins 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000002411 thermogravimetry Methods 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013325 dietary fiber Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003921 particle size analysis Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001592 potato starch Polymers 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 238000000113 differential scanning calorimetry Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002608 ionic liquid Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the field of animal nutrition and feed resource development, in particular to a method for strengthening degradation of sweet potato residue fiber 3 Processing, and performing extrusion puffing operation to enhance the degradation of sweet potato residue fiber,in the extrusion process, naHCO is used synergistically 3 And glycerine to enhance the extrusion effect by heating NaHCO 3 CO produced 2 The internal pressure is improved, and the glycerol with high boiling point and specific heat performance is used for improving the extrusion temperature, so that the degradation capability of cellulose and hemicellulose can be obviously improved, the yield of glucose and xylose is obviously improved, the additional value of sweet potato residue is improved, and the extrusion puffing effect is obviously improved.
Description
Technical Field
The invention relates to the field of animal nutrition and feed resource development, in particular to a method for enhancing the degradation of sweet potato residue fibers.
Background
Sweet potato is one of food crops in China, the planting area and the yield of the sweet potato are in the top of the world, sweet potato residues are byproducts generated in the sweet potato starch extraction process, account for 10% -14% of the total amount of the sweet potato starch, are discharged along with wastewater by many enterprises, contain a large amount of nutrient substances including starch, dietary fiber, protein and the like, wherein the starch content accounts for 48.26%, the dietary fiber accounts for 26.73%, and the protein accounts for 3.35%, and deep processing and comprehensive utilization of the sweet potato residues can reduce environmental pollution, increase the economic value of the sweet potato residues and realize sustainable utilization of resources.
The sweet potato is used as an important food crop in China, is mainly used for producing starch, can generate a large amount of sweet potato residues (1000 ten thousand tons/year), contains rich fiber resources, but is used for animal feeding in a small amount (within 10 percent), and most of the sweet potato residues are directly discarded, so that serious environmental pollution and resource waste are caused. Obviously, the efficient development and utilization of the sweet potato residues are an important way for solving the energy and environmental crisis. The sweet potato residue is composed of cellulose, hemicellulose and lignin, and the cellulose forms a three-dimensional network structure with the lignin and the hemicellulose due to strong hydrogen bonds, high polymerization degree and high crystallinity, so that how to efficiently hydrolyze the cellulose and the hemicellulose is the key of utilizing sweet potato residue fiber resources.
The sweet potato residue fiber degradation method in the prior art mainly comprises acidolysis, alkaline hydrolysis, thermochemical treatment, ionic liquid and the like, but the methods have the defects of toxic compound generation, long processing time, serious corrosion to production equipment and the like in the pretreatment process, so that the application of the pretreatment method is limited, and on the basis, the sweet potato residue fiber degradation method which is simple and convenient to operate, large in processing scale and free of pollution is needed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for enhancing the fiber degradation of sweet potato residues.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for enhancing degradation of sweet potato residue fiber comprises subjecting sweet potato residue fiber to glycerol and NaHCO 3 Processing, and performing extrusion puffing operation in cooperation to enhance the degradation of the sweet potato residue fiber;
preferably, the method for enhancing the fiber degradation of the sweet potato residue comprises the following steps:
(1) Sequentially carrying out purification, drying and crushing pretreatment on the sweet potato residues to obtain treated sweet potato residues;
(2) Adding sweet into the sweet potato residue treated in the step (1)Oil, naHCO 3 Mixing with water, and stirring to obtain mixed sweet potato residue;
wherein the sweet potato residue contains 20-30% of water, 1-10% of glycerol and NaHCO 3 The mass to water volume ratio of (1-2 g): 1L;
(3) And (3) treating the mixed sweet potato residues by using a double-screw extrusion bulking machine, jointly hydrolyzing the sweet potato residues before extrusion and after extrusion by using cellulase and hemicellulase, and performing post-treatment to obtain the degraded sweet potato residue fiber.
Preferably, the sweet potato residue contains 25% of water, 5% of glycerin by mass and NaHCO 3 The mass to water volume ratio of (2) is 1g/L.
Preferably, the pretreatment of the sweet potato residue in the step (1) comprises the following specific steps: cleaning the sweet potato residue with water, drying to constant weight, crushing, and sieving to obtain a sweet potato residue sample.
Preferably, the screw rotating speed of the twin-screw extrusion bulking machine in the step (3) is 100-140rpm, the extrusion temperature is 110-150 ℃, and the feeding speed is 15-19Hz.
Preferably, the storage post-treatment method in step (3) comprises: drying to constant weight, sieving, sealing, drying and storing.
Preferably, the drying temperature in the step (1) and the drying time in the step (3) are both 50-70 ℃, the drying time is 24-72 hours, and the drying temperature and the drying time are both screened by adopting a 30-50 mesh screen.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention uses NaHCO synergistically in the extrusion process 3 And glycerine to enhance the extrusion effect by heating NaHCO 3 CO produced 2 The internal pressure is increased, and the glycerol with high boiling point and specific heat performance is used for increasing the extrusion temperature, so that the degradation capability of cellulose and hemicellulose is obviously improved, the yield of glucose and xylose is obviously increased, the additional value of sweet potato residue is obviously increased, and the extrusion puffing effect is obviously improved;
2. compared with the traditional chemical fiber degradation process, the extrusion and puffing process obviously improves the reduction of the cellulose and the hemicellulase to the sweet potato residue fiberThe dissolving capacity, namely the spatial network structure of cellulose and hemicellulose is destroyed through extrusion and puffing; meanwhile, the grain size of the sweet potato residue after the extrusion and expansion treatment is reduced, the specific surface area contacted with the enzyme is increased, and more enzyme digestion sites are released, so that the hydrolysis rate of the enzyme is improved; by glycerol and NaHCO 3 The extrusion puffing process is synergistically enhanced, the yield of monosaccharide is improved, the energy consumption is reduced, the production cost is saved, the environmental pollution is reduced, the additional value of the sweet potato residue is obviously improved, and the extrusion puffing effect is obviously improved;
3. when the water content of the sweet potato residue is 30%, the puffing effect is good, and if the water content is low, the puffing of a sample is influenced, so that a discharge port is easily blocked; the water content is too high, the instant pressure of sample expansion can be reduced, the expansion effect can be influenced, the production cost is increased, meanwhile, the glycerol with the replacement mass fraction of 5 percent also has the best expansion effect, and NaHCO is used for replacing 3 The difference between 1 and 2g/L is not significant, so 1g/L is taken.
Drawings
Fig. 1 is a thermal stability curve a for the sweet potato residue samples of example 1, comparative example 1 and comparative example 2: comparative example 2; b: comparative example 1; c: example 1;
fig. 2 is a scanning electron micrograph a of the sweet potato residue samples of example 1, comparative example 1 and comparative example 2: comparative example 2; b: comparative example 1; c: example 1;
FIG. 3 is an infrared spectrum of a sample of sweet potato residue of example 1, comparative example 1 and comparative example 2;
FIG. 4 is an X-ray diffraction pattern of samples of sweet potato residue of example 1, comparative example 1 and comparative example 2;
fig. 5 is a graph of glucose (a) and xylose (B) yields for the sweet potato residue samples of example 1, comparative example 1 and comparative example 2.
Detailed Description
The following detailed description is provided to best modes for carrying out the invention and is to be read in connection with the accompanying drawings.
Example 1
A method for enhancing fiber degradation of sweet potato residue comprises the following steps:
(1) Thoroughly cleaning sweet potato residue with water, drying at 60 deg.C for 48 hr to constant weight, pulverizing, and sieving with 40 mesh sieve to obtain treated sweet potato residue, which is named as SPRs;
(2) Adding 25 percent of distilled water, 5 percent of glycerin and 1g/LNaHCO into the sweet potato residues treated by the SPRs in the step (1) 3 And mixing and stirring uniformly to obtain mixed sweet potato residues named as SPRs-DW-GNa;
(3) And (3) extruding the SPRs-DW-GNa sweet potato residue sample obtained in the step (2) by using a double-screw extrusion and expansion machine, wherein the rotating speed of a screw is 120rpm, the extrusion temperature is 130 ℃, the feeding speed is 17Hz, the extruded sweet potato residue sample is dried at 60 ℃ for 48h to constant weight, and the dried sweet potato residue sample is screened by a 40-mesh screen to obtain the degraded sweet potato residue fiber.
Example 2
A method for enhancing fiber degradation of sweet potato residue comprises the following steps:
(1) Thoroughly washing sweet potato residue with water, drying at 50 deg.C for 24 hr to constant weight, pulverizing, and sieving with 30 mesh sieve to obtain processed sweet potato residue;
(2) Adding 20% of distilled water, 1% of glycerol and 1g/LNaHCO into the sweet potato residue treated in the step (1) 3 And mixing and stirring uniformly to obtain mixed sweet potato residues;
(3) And (3) extruding the mixed sweet potato residue sample obtained in the step (2) by using a double-screw extrusion puffing machine, wherein the rotating speed of a screw is 100rpm, the extrusion temperature is 110 ℃, the feeding speed is 15Hz, the extruded sweet potato residue sample is dried for 24 hours at 50 ℃ to constant weight, and the sample is sieved by a 30-mesh screen to obtain the degraded sweet potato residue.
Example 3
A method for enhancing fiber degradation of sweet potato residue comprises the following steps:
(1) Thoroughly cleaning sweet potato residue with water, drying at 60 deg.C for 48 hr to constant weight, pulverizing, and sieving with 40 mesh sieve;
(2) Adding 30 mass percent of distilled water, 10 mass percent of glycerin and 2g/LNaHCO into the SPRs sweet potato residue sample in the step (1) 3 And mixing and stirring uniformly to obtain mixed sweet potato residues;
(3) And (3) extruding the mixed sweet potato residue sample obtained in the step (2) by using a double-screw extrusion puffing machine, wherein the rotating speed of a screw is 140rpm, the extrusion temperature is 150 ℃, the feeding speed is 19Hz, drying the extruded sweet potato residue sample at 70 ℃ for 72h to constant weight, and screening the dried sweet potato residue sample by using a 50-mesh screen to obtain the degraded sweet potato residue.
Comparative example 1
30% distilled water, named as SPRs-DW, was added to the sweet potato residue sample of step (1) in example 1, and the sample was processed using a twin-screw extrusion-expansion machine, and then subjected to sample testing using the same testing method as in example 1.
Comparative example 2
The same procedure as in example 1 was followed, except that the detection was carried out using the samples of SPRs obtained in step (1) of example 1.
The characterization method of the invention comprises the following steps:
research on physicochemical characteristics of sweet potato residues before and after extrusion
1. Particle size analysis
And analyzing the particle size of the sweet potato residue sample before and after extrusion by using a Malvern 3000 particle size analyzer, and evaluating the uniformity of the sweet potato residue through particle span.
2. Chemical composition
The crude protein content of the sweet potato residue sample before and after extrusion is determined by adopting an AOAC 920.87 method, the crude fat content is determined by adopting an AACC 30-25 method, and the crude ash content is determined by adopting an AOAC 923.03 method.
3. Thermogravimetric analysis (TG)
And (3) placing the sweet potato residue samples before and after extrusion in an instrument, setting the flow of protective gas nitrogen at 20mL/min, heating the furnace body from 25 ℃ to 800 ℃ at a heating rate of 20 ℃/min, and deducting a blank curve to obtain a thermogravimetric experiment curve (TG) and a micro-entropy thermogravimetric curve (DTG).
4. Differential Scanning Calorimetry (DSC)
And (3) placing the sweet potato residue sample before and after extrusion into an instrument, setting the flow of protective gas nitrogen at 20mL/min, heating the furnace body from 25 ℃ to 800 ℃ at a heating rate of 20 ℃/min, and deducting a blank curve to obtain a thermogravimetric experiment curve (TG) and a microentropy thermogravimetric curve (DTG).
Research on structural characteristics of sweet potato residues before and after extrusion
1. Scanning electron microscope
And (3) putting the sweet potato residue samples before and after extrusion into an ion sputtering coating instrument, coating a layer of gold film with the thickness of about 100nm on the surface of the sample under vacuum, and observing the morphological characteristic change of the sweet potato residue samples before and after extrusion by using a scanning electron microscope.
2. Fourier infrared spectroscopy
The method comprises the following steps of (1) placing sweet potato residue samples before and after extrusion under an infrared lamp, avoiding the samples from absorbing moisture, and mixing the sweet potato residue samples with dry KBr powder according to a mass ratio of 1:100 are mixed in an agate mortar, ground evenly and finely until no granular sensation exists, added into a pressing die and pressed into a sheet and immediately placed in a light path for scanning, and the scanning interval is 400-4000 cm -1 。
X-ray diffraction analysis
The crystal structure of the sweet potato residue samples before and after extrusion was determined using an X' Pert 3 Powedr X-ray diffractometer. The experimental condition parameters are as follows: the voltage is 40kV, and the current is 40mA; the starting angle is 5 deg., and the ending angle is 70 deg..
Research on enzymolysis effect of sweet potato residues before and after extrusion
1. Enzymatic analysis
Hydrolyzing the sweet potato residues before and after extrusion by using cellulase (10000U/g) and hemicellulase (10000U/g), adding sodium azide with the mass concentration of 0.02% to inhibit the growth of microorganisms by using a 250mL conical flask with the pH =4.8 and containing 50mL of sodium acetate buffer solution, adding 1g of sweet potato residues into each conical flask, wherein the enzyme loading amount is 15FPU/g dry weight, oscillating the conical flask at 50 ℃ and 150rpm for 72h, taking 5mL of enzymatic hydrolysate to determine the contents of glucose and xylose at intervals of 12h, carrying out enzymolysis, filtering the sweet potato residues, washing the sweet potato residues with distilled water for 5 times, drying the sweet potato residues at 80 ℃ to constant weight, and determining the contents of cellulose, hemicellulose and lignin in the sweet potato residues after the enzymolysis by using a fiber analyzer.
2. Glucose and xylose production assays
The enzymatic hydrolysate was centrifuged at 12000g at 4 ℃ for 10min, and the glucose and xylose yields in the supernatant were determined by high performance liquid chromatography with a differential refractometer (HPLC, HPX-87H column, 300mm. Times.7.8 mm), HPLC conditions: the mobile phase was 5mM H 2 SO 4 The flow rate is 0.6mL/min, the temperature is 35 ℃, the sample loading amount is 20uL, and the sample solution is filtered by a 0.22um filter membrane before sample loading.
Results and analysis
Analysis of physicochemical Properties of sweet Potato residue samples before and after extrusion
1. Particle size analysis
The average particle size and specific surface area of the sweet potato residue samples before and after extrusion are shown in Table 1, compared with the groups of the SPRs, the surface area average particle size (D3, 2) and the volume average particle size (D4, 3) of the groups of the SPRs-DW and the SPRs-DW-GNa are respectively reduced, and the specific surface area is obviously increased, which indicates that the extrusion treatment reduces the particle size of the sweet potato residue and improves the specific surface area of the sample, and the reason is that the macromolecular polymer of the sweet potato residue is degraded due to the extrusion treatment.
TABLE 1 analysis of mean particle size and specific surface area of sweet potato residue samples before and after extrusion
| Sample (I) | D3,2(μm) | D4,3(μm) | Specific surface area (m) 2 /g) |
| SPRs | 37.643 | 173.005 | 0.159 |
| SPRs-DW | 34.716 | 165.973 | 0.173 |
| SPRs-DW-Gna | 34.794 | 153.441 | 0.172 |
2. Chemical composition analysis
Chemical components of the sweet potato residue sample before and after extrusion are shown in Table 2, cellulose contents of different sweet potato residue samples after extrusion are respectively 13.5% (SPRs-DW) and 13% (SPRs-DW-GNa), and compared with an original sweet potato residue sample (13.9%), the cellulose contents are respectively reduced by 2.87% and 6.5%; in addition, the hemicellulose content of the sweet potato residue sample is reduced from 9.2 percent (SPRs) to 8.4 percent (SPRs-DW) and 7.7 percent (SPRs-DW-GNa), respectively by 8.7 percent (SPRs-DW) and 16.3 percent (SPRs-DW-GNa), and the lignin content of the sweet potato residue sample is reduced from 4.6 percent (SPRs) to 4.3 percent (SPRs-DW) and 4.0 percent (SPRs-DW-GNa), respectively by 6.5 percent (SPRs-DW) and 13 percent (SPRs-DW-GNa), so that the cellulose, hemicellulose and lignin components in the sweet potato residue sample can be remarkably degraded by extrusion and expansion treatment, wherein 25 percent of distilled water, 5 percent of glycerol and 1g/L of NaHCO are added into the sweet potato residue sample 3 The treatment group (SPRs-DW-GNa) performed best.
TABLE 2 chemical composition (%, dry basis) of sweet potato residue samples before and after extrusion
| Sample (I) | Cellulose, process for producing the same, and process for producing the same | Hemicellulose | Lignin | Starch | Crude fat | Crude protein | Coarse ash content |
| SPRs | 13.9 | 9.2 | 4.6 | 54.28 | 0.84 | 3.6 | 2.54 |
| SPRs-DW | 13.5 | 8.4 | 4.3 | 53.82 | 0.72 | 3.1 | 3.37 |
| SPRs-DW-GNA | 13 | 7.7 | 4.0 | 52.93 | 0.54 | 3.29 | 7.68 |
3. Analysis of thermal stability
The TG curve shows the presence of multi-stage thermal degradation of the sample of the sweet potato residue before and after extrusion (fig. 1), wherein the sweet potato residue before and after extrusion shows a first degradation peak at 58.19-66.18 ℃, at this stage, the moisture loss of the sweet potato residue may be the main cause of mass loss, and as the temperature rises, the carbohydrates begin to degrade, and the final products are C, CO and combustible volatile substances, thereby causing endothermic degradation to be converted into exothermic process, in the DSC spectrum, the peak is endothermic upward and the peak is downward; compared with the untreated sweet potato residue, the sweet potato residue after extrusion has smaller endothermic peak and exothermic peak, which indicates that the extrusion enhances the thermal stability of the polymer.
Analysis of structural characteristics of sweet potato residue samples before and after extrusion
1. Scanning electron microscope
The microstructure of the sweet potato residue sample before and after extrusion is shown in figure 2 (2000 times), the surface of the untreated sweet potato residue sample is flaky, which shows that the structure and the fiber structure of the sweet potato residue are kept intact (figure 2A), the fiber particles of the SPRs-DW become smaller and the cracks increase (figure 2B) after the sample is added with 30% of distilled water and is extruded, and when 25% of distilled water, 5% of glycerol and 1g/L of NaHCO are used 3 When extrusion treatment is carried out, the SpRs-DW-GNa fibers are finely crushed and have rough surfaces (figure 1C), and the observation results show that the extrusion treatment effectively breaks the stubborn structure of the sweet potato residue and is beneficial to subsequent enzymolysis, in addition, macromolecules can change into smaller soluble molecules in the extrusion process, and certain honeycomb appearance is presented, because of the cracking of glycosidic bonds and the degradation of IDF macromolecules under the extrusion action, in a word, the crushing effect of the SPRs-DW-GNa on the sweet potato residue fibers is better than that of the SPRs-DW and the SPRs.
2. Fourier infrared spectrum
Fourier infrared spectroscopy is utilized to analyze the difference of the functional group compositions of the sweet potato residue sample before and after extrusion (figure 3), and the difference is 3000cm -1 The absorption peak at (C-H) was probably due to stretching of = C-H in cellulose, at 3000cm for the groups of SPRs-DW and SPRs-DW-GNa compared to the untreated sweet potato residue group -1 The absorption peak is reduced, which shows that the cellulose content in the sweet potato residue sample is reduced after the extrusion treatment, and the cellulose reduction of the SPRs-DW-GNa treatment group is most obvious, and in addition, 1300cm -1 The absorption peak at (A) represents C-O vibration in lignin, 1376cm -1 The absorption peak at (A) may be related to C-H vibration is related, and compared with untreated sweet potato residue group, the groups of the SPRs-DW and the SPRs-DW-GNa are 1376cm -1 The absorption peak is reduced, which shows that the content of hemicellulose in the sweet potato residue sample is reduced after extrusion treatment, and the reduction effect of the SPRs-DW-GNa group is more obvious, which is consistent with the chemical composition result of the sweet potato residue in the table 2.
X-ray diffraction analysis
The X-ray diffraction spectra of the sweet potato residue samples before and after extrusion are shown in figure 4, and compared with the untreated sweet potato residue group, the diffraction peaks of the SPRs-DW and the SPRs-DW-GNa groups show a descending trend at about 20 degrees, which indicates that the crystallization index of the sweet potato residue after extrusion treatment is reduced, and the X-ray diffraction spectra are mainly related to the damage of hydrogen bonds in cellulose and the breakage of a compact structure of hemicellulose in the extrusion process, and the damage effect of the SPRs-DW-GNa group is more obvious, which is consistent with the chemical composition result of the sweet potato residue in the table 2.
4. Research on enzymolysis effect of sweet potato residues before and after extrusion
In the invention, the glucose yield of an untreated sweet potato residue sample after 48 hours of enzymolysis is 19.749g/L (figure 5A), the glucose yield of the untreated sweet potato residue sample after 72 hours of enzymolysis reaches 31.588g/L, the glucose yields of the groups SPRs-DW and SPRs-DW-GNa after extrusion are respectively 28.794 and 35.251g/L at 48 hours, the glucose yields of the groups SPRs-DW and SPRs-DW-GNa are respectively improved by 45.8% and 78.5% compared with the untreated sweet potato residue sample, the glucose yields of the groups SPRs-DW and SPRs-DW-GNa after 72 hours are respectively 43.936 and 44.925g/L, the xylose yields of the groups SPRs, SPRs-DW and SPDW-GNa are respectively improved by 39% and 42.2% compared with the untreated sweet potato residue sample, and the xylose yields of the groups SPRs, SPRs-DW and SPRs, SPDW-GNa and SPRs, 48.221 g and 10.8% compared with the glucose yields of the groups after 72 hours of enzymolysis, the glucose yields of the groups are respectively improved by 48.438 g/L and 48 h; and the glucose yield of the groups of the SPRs-DW and the SPRs-DW-GNa is 11.696 and 12.668g/L respectively at 72h, which are respectively improved by 34.2 percent and 45.3 percent compared with an untreated sweet potato residue sample.
In conclusion, 25% of distilled water, 5% of glycerol and 1g/LNaHCO are added into the sweet potato residue sample 3 After the treatment (SPRs-DW-GNa), the degradation effect on cellulose, hemicellulose and lignin of the sweet potato residue is optimal, and at the moment, the grain size of a sweet potato residue sample is reduced, the specific surface area is increased, the crystallinity is reduced, the thermal stability is enhanced, and the enzymolysis effect is optimal.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. A method for strengthening the degradation of sweet potato residue fiber is characterized in that the sweet potato residue fiber is firstly treated by adopting glycerol and NaHCO 3 And (4) processing, and performing extrusion and puffing operation in cooperation to strengthen the degradation of the sweet potato residue fiber.
2. The method for enhancing fiber degradation of sweet potato residue as claimed in claim 1, comprising the steps of:
(1) Sequentially carrying out purification, drying and crushing pretreatment on the sweet potato residue to obtain treated sweet potato residue;
(2) Adding glycerol and NaHCO into the sweet potato residue treated in the step (1) 3 Mixing with water, and stirring to obtain mixed sweet potato residue;
wherein the sweet potato residue contains 20-30% of water, 1-10% of glycerol and NaHCO 3 1-2g of mass to volume ratio of water: 1L;
(3) And (3) processing the mixed sweet potato residue obtained in the step (2) by using a double-screw extrusion puffing machine, and storing and post-processing the extruded sweet potato residue to obtain the degraded sweet potato residue fiber.
3. The method for enhancing fiber degradation of sweet potato residue according to claim 2, wherein the sweet potato residue of step (2) contains 25% water, 5% glycerin, and NaHCO 3 The mass to volume ratio of water of (1) to (1 g/L).
4. The method for enhancing fiber degradation of sweet potato residue according to claim 2, wherein the pretreatment of the sweet potato residue in the step (1) comprises the following specific steps: cleaning the sweet potato residue with water, drying to constant weight, crushing, and sieving to obtain a sweet potato residue sample.
5. The method for enhancing fiber degradation of sweet potato residue according to claim 2, wherein in the step (3), the rotation speed of the screw of the twin-screw extrusion-expansion machine is 100-140rpm, the extrusion temperature is 110-150 ℃, and the feeding speed is 15-19Hz.
6. The method for enhancing fiber degradation of sweet potato residue according to claim 2, wherein the post-storage treatment method in step (3) is as follows: drying to constant weight, sieving, sealing, drying and storing.
7. The method for enhancing the fiber degradation of the sweet potato residue as claimed in claim 6, wherein the drying temperature in step (1) and the drying time in step (3) are 50-70 ℃, the drying time is 24-72 hours, and the drying temperature and the drying time are both sieved by a 30-50 mesh sieve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211226538.5A CN115636979B (en) | 2022-10-09 | 2022-10-09 | Method for strengthening degradation of sweet potato residue fibers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211226538.5A CN115636979B (en) | 2022-10-09 | 2022-10-09 | Method for strengthening degradation of sweet potato residue fibers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115636979A true CN115636979A (en) | 2023-01-24 |
| CN115636979B CN115636979B (en) | 2024-01-26 |
Family
ID=84942050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211226538.5A Active CN115636979B (en) | 2022-10-09 | 2022-10-09 | Method for strengthening degradation of sweet potato residue fibers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115636979B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105011021A (en) * | 2014-04-30 | 2015-11-04 | 湖南农业大学 | Sweet potato and bean pulp extrusion expansion flour food |
| CN106509627A (en) * | 2016-10-13 | 2017-03-22 | 张家界天湘薯业有限公司 | Efficient utilization method of sweet potato residue |
| CN110074419A (en) * | 2019-05-17 | 2019-08-02 | 山东理工大学 | Squeeze the method that enzymatic hydrolysis pea slag prepares soluble dietary fiber |
| US20190249107A1 (en) * | 2016-12-30 | 2019-08-15 | Jiangnan University | Method for Co-Production and Processing of Biological Energy Sources by Oil Crops |
-
2022
- 2022-10-09 CN CN202211226538.5A patent/CN115636979B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105011021A (en) * | 2014-04-30 | 2015-11-04 | 湖南农业大学 | Sweet potato and bean pulp extrusion expansion flour food |
| CN106509627A (en) * | 2016-10-13 | 2017-03-22 | 张家界天湘薯业有限公司 | Efficient utilization method of sweet potato residue |
| US20190249107A1 (en) * | 2016-12-30 | 2019-08-15 | Jiangnan University | Method for Co-Production and Processing of Biological Energy Sources by Oil Crops |
| CN110074419A (en) * | 2019-05-17 | 2019-08-02 | 山东理工大学 | Squeeze the method that enzymatic hydrolysis pea slag prepares soluble dietary fiber |
Non-Patent Citations (1)
| Title |
|---|
| 单成俊;周剑忠;黄开红;王英;李莹;: "挤压膨化提高甘薯渣中可溶性膳食纤维含量的研究", 江西农业学报, no. 06, pages 94 - 95 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115636979B (en) | 2024-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2006354A2 (en) | Novel method for production liquid fuel from biomass | |
| Cui et al. | Influence of steam explosion pretreatment on the composition and structure of wheat straw | |
| JP6078060B2 (en) | Conditioning of biomass to improve C5 / C6 sugar release before fermentation | |
| EP2343379A1 (en) | Saccharide production process and ethanol production process | |
| Yang et al. | Application of engineered yeast strain fermentation for oligogalacturonides production from pectin-rich waste biomass | |
| Ji et al. | Production of xylose from diluted sulfuric acid hydrolysis of wheat straw | |
| CN107557404A (en) | A kind of method for improving agricultural stalk raw material full constituent utilization rate | |
| US20060141584A1 (en) | Pretreatment of biomass for ethanol production | |
| Zhou et al. | Near-complete enzymatic hydrolysis efficiency of Miscanthus using hydrotropic fractionation at atmospheric pressure | |
| Tao et al. | Thermo-carbide slag pretreatment of energy plants for enhancing enzymatic hydrolysis | |
| Zhuang et al. | Decomposition behavior of hemicellulose and lignin in the step-change flow rate liquid hot water | |
| Zhang et al. | Revalorization of sunflower stalk pith as feedstock for the coproduction of pectin and glucose using a two-step dilute acid pretreatment process | |
| Chang et al. | Impact of double alkaline peroxide pretreatment on enzymatic hydrolysis of palm fibre | |
| Cui et al. | Effects of microwave-assisted liquid hot water pretreatment on chemical composition and structure of moso bamboo | |
| Stankovska et al. | Effect of alkaline extrusion pretreatment of wheat straw on filtrate composition and enzymatic hydrolysis | |
| Cui et al. | Surfactant-assisted ethylenediamine for the deconstruction and conversion of corn stover biomass | |
| CN110130135B (en) | Method for pretreating lignocellulose raw material by using sodium acetate and sodium sulfite | |
| CN115636979A (en) | Method for enhancing fiber degradation of sweet potato residue | |
| JPS6151880B2 (en) | ||
| WO2012113990A1 (en) | Process for the mechanical or mechano-chemical pretreatment of biomass | |
| Fatmawati et al. | Hydrolysis of alkaline pretreated banana peel | |
| Wang et al. | Combined mechanical destruction and alkaline pretreatment of wheat straw for enhanced enzymatic saccharification | |
| Gong et al. | Enhanced enzymolysis and bioethanol yield from tobacco stem waste based on mild synergistic pretreatment | |
| Jiang et al. | Optimization of dilute NaOH pretreatment at mild temperatures for monomeric sugar release from sorghum pith using response surface methodology | |
| Qiao et al. | Recycling of spent liquor for treating corn cobs to create digestible cellulose and enrich the xylooligosaccharide concentration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |